Molecular marker, detection reagent and method for breeding leizhou goat and application thereof

Abstract

The application provides a molecular marker, a detection reagent, a method and an application thereof for breeding Leizhou goats. CDK14 The application screens a SNP site and a haplotype molecular marker which are significantly related to the body size trait of the Leizhou goats in exon 10 of the gene, and the SNP site and the haplotype molecular marker can be applied to early assisted selection for breeding the Leizhou goats. The application further provides a detection reagent and a detection method for detecting the SNP site and the haplotype molecular marker, and the body size trait of the Leizhou goats can be quickly, effectively and accurately predicted, so that the production cost is saved and the breeding process is accelerated, and the application has great economic application value and scientific research value.

Description

Technical Field

[0001] This invention relates to the technical field of molecular breeding, and more specifically, to molecular markers, detection reagents, methods, and their applications for breeding Leizhou goats. Background Technology

[0002] Leizhou goats are mainly distributed in the Leizhou Peninsula of Guangdong and Hainan Island. They are one of the superior breeds in western Guangdong and a major meat goat breed in the province. However, the diverse farming methods and inconsistent management practices, coupled with a late start in the protection and development of genetic resources, have led to severe breed degradation and inconsistent traits in recent years. Furthermore, the transition from small-scale to standardized large-scale farming has exposed problems such as reduced adaptability and weakened resistance in Leizhou goats, posing significant challenges to the high-quality development of the Leizhou goat industry and its genetic breeding efforts. In Leizhou goat breeding, selecting high-performing individuals is crucial. Body size traits in goats include height, body length, cross-shaped height, chest circumference, and cannon bone circumference, directly reflecting the goat's growth and body structure. Currently, there are relatively few loci and identification techniques related to body size traits in Leizhou goats, which is insufficient to meet the needs of the farming industry. Therefore, it is necessary to find more loci related to body size traits in Leizhou goats to establish a rapid and convenient molecular breeding technique for selecting superior individuals.

[0003] Cyclin-dependent kinase 14 (CDK14) belongs to the TAIRE subfamily of the CDK family (also known as atypical CDKs) and is a serine / threonine protein kinase involved in cell cycle progression and cell proliferation regulation. In goats, the CDK14 gene is located on chromosome 4 (chr4: 111953410–112626647 bp), containing 16 exons. Three transcript variants of this gene have been identified in the NCBI GenBank database. Early research on the CDK14 gene mainly focused on malignant tumors; this gene is also a member of the Wnt signaling pathway. Studies have shown that the CDK14 / PFTK1-cyclin Y complex can activate the atypical Wnt pathway, thereby activating Rho guanosine triphosphatases (Rho GTPases) for cell migration. Current research on CDK14 focuses on its effects on animal production and reproductive traits, while its effects on body size traits in goats remain unknown. Summary of the Invention

[0004] To overcome the aforementioned defects and shortcomings in the existing technology, this invention provides molecular markers, detection reagents, methods, and their applications for breeding Leizhou goats.

[0005] The first objective of this invention is to provide the application of a reagent for detecting SNP molecular markers used in the breeding of Leizhou goats in the preparation of Leizhou goat breeding products.

[0006] A second objective of this invention is to provide the application of a reagent for detecting haplotype SNP molecular markers used in the breeding of Leizhou goats in the preparation of products related to Leizhou goat breeding.

[0007] The third objective of this invention is to provide a method for breeding Leizhou goats.

[0008] This invention claims protection for the following:

[0009] The application of reagents for detecting SNP molecular markers used in the breeding of Leizhou goats in the preparation of Leizhou goat breeding products, wherein the SNP molecular markers consist of 9 single nucleotide polymorphism sites SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNP8 and / or SNP9.

[0010] SNP1 is located at position 112210991 on NC_030811.1 and is a C or T polymorphism, with three genotypes: CC, CT, and TT.

[0011] SNP2 is located at position 112210978 on NC_030811.1 and is a C or G polymorphism, with three genotypes: CC, CG, and GG.

[0012] SNP3 is located at position 112210954 on NC_030811.1 and is a C or T polymorphism, with three genotypes: CC, CT, and TT.

[0013] SNP4 is located at position 112210869 on NC_030811.1 and is a T or G polymorphism, with three genotypes: TT, TG, and GG.

[0014] SNP5 is located at position 112210845 on NC_030811.1 and is a T or C polymorphism, with three genotypes: TT, TC, and CC.

[0015] SNP6 is located at position 112210753 on NC_030811.1 and is a T or A polymorphism, with three genotypes: TT, TA, and AA.

[0016] SNP7 is located at position 112210669 on NC_030811.1 and is a G or A polymorphism, with three genotypes: GG, GA, and AA.

[0017] SNP8 is located at position 112210653 on NC_030811.1 and is a C or T polymorphism, with three genotypes: CC, CT, and TT.

[0018] SNP9 is located at position 112210652 on NC_030811.1 and is an A or G polymorphism, with three genotypes: AA, AG, and GG.

[0019] Individuals with the SNP1 locus genotype TT had significantly higher chest height, chest width, or chest circumference than individuals with the genotype CT or CC. Individuals with the genotype TT had significantly higher chest depth than individuals with the genotype CT. Individuals with the genotype TT had significantly higher canal circumference than individuals with the genotype CC.

[0020] Individuals with the SNP2 locus genotype GG had significantly higher cross height, chest width, or chest circumference than individuals with the genotypes CG or CC. Individuals with the genotype GG had significantly higher chest depth than individuals with the genotype CG, and individuals with the genotype GG had significantly higher canal circumference than individuals with the genotype CC.

[0021] Individuals with the SNP3 locus genotype TT had significantly higher chest height, chest width, or chest circumference than individuals with the genotype CT or CC. Individuals with the genotype TT had significantly higher chest depth than individuals with the genotype CT. Individuals with the genotype TT had significantly higher canal circumference than individuals with the genotype CC.

[0022] Individuals with the SNP4 locus genotype GG had significantly higher cross height, chest width, or chest circumference than individuals with the genotype TG or TT. Individuals with the genotype GG had significantly higher chest depth than individuals with the genotype TG. Individuals with the genotype GG had significantly higher canal circumference than individuals with the genotype TT.

[0023] Individuals with the SNP5 genotype CC had significantly higher body height or cross height than individuals with the genotype TC. Individuals with the genotype CC had significantly higher chest depth, chest width, chest circumference, or cannon bone circumference than individuals with the genotype TC or TT.

[0024] Individuals with the SNP6 locus genotype AA had significantly higher body height or cross height than individuals with the genotype TA. Individuals with the genotype AA had significantly higher chest depth or cannon circumference than individuals with the genotype TA or TT. Individuals with the genotype AA had significantly higher chest circumference than individuals with the genotype TT.

[0025] Individuals with the SNP7 locus genotype AA had significantly higher cross height, chest width, or chest circumference than individuals with the genotype GA or GG; individuals with the genotype AA had significantly higher chest depth than individuals with the genotype GA; and individuals with the genotype AA had significantly higher canal circumference than individuals with the genotype GG.

[0026] Individuals with the SNP8 locus genotype TT had significantly higher cross height, chest width, or chest circumference than individuals with the genotype CT or CC. Individuals with the genotype TT had significantly higher chest depth than individuals with the genotype CT. Individuals with the genotype TT had significantly higher canal circumference than individuals with the genotype CC.

[0027] Individuals with the SNP9 genotype GG had significantly higher chest height, chest width, or chest circumference than individuals with the genotypes AG or AA. Individuals with the genotype GG had significantly higher chest depth than individuals with the genotype AG. Individuals with the genotype GG had significantly higher canal circumference than individuals with the genotype AA.

[0028] The application of reagents for detecting haplotype SNP molecular markers used in the breeding of Leizhou goats in the preparation of products for the breeding of Leizhou goats, wherein the haplotype SNP molecular markers are constructed from nine single nucleotide polymorphism sites SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNP8 and SNP9;

[0029] SNP1 is located at position 112210991 on NC_030811.1 and is a C or T polymorphism, with three genotypes: CC, CT, and TT.

[0030] SNP2 is located at position 112210978 on NC_030811.1 and is a C or G polymorphism, with three genotypes: CC, CG, and GG.

[0031] SNP3 is located at position 112210954 on NC_030811.1 and is a C or T polymorphism, with three genotypes: CC, CT, and TT.

[0032] SNP4 is located at position 112210869 on NC_030811.1 and is a T or G polymorphism, with three genotypes: TT, TG, and GG.

[0033] SNP5 is located at position 112210845 on NC_030811.1 and is a T or C polymorphism, with three genotypes: TT, TC, and CC.

[0034] SNP6 is located at position 112210753 on NC_030811.1 and is a T or A polymorphism, with three genotypes: TT, TA, and AA.

[0035] SNP7 is located at position 112210669 on NC_030811.1 and is a G or A polymorphism, with three genotypes: GG, GA, and AA.

[0036] SNP8 is located at position 112210653 on NC_030811.1 and is a C or T polymorphism, with three genotypes: CC, CT, and TT.

[0037] SNP9 is located at position 112210652 on NC_030811.1 and is an A or G polymorphism, with three genotypes: AA, AG, and GG.

[0038] The SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNP8, and SNP9 construct four haplotypes:

[0039] The H3 haplotype is CCCTTGCA, the H4 haplotype is TGTGCAATG, the H5 haplotype is TGTGTTATG, and the H6 haplotype is TGTGCTATG.

[0040] Individuals with the H4 haplotype had significantly higher chest depth, chest circumference, or cannon circumference than individuals with the H3 haplotype.

[0041] The haplotypes construct haplotype combinations, which include the H3H3 haplotype combination, the H3H4 haplotype combination, the H3H6 haplotype combination, the H4H4 haplotype combination, and the H4H5 haplotype combination.

[0042] The cross-shaped height of individuals in the H3H4 haplotype combination was significantly lower than that of individuals in the H4H4 haplotype combination;

[0043] Individuals with the H3H3, H3H4, or H3H6 haplotype combinations had significantly lower chest depths than individuals with the H4H4 haplotype combination.

[0044] Individuals with the H3H3 haplotype combination had significantly lower chest width than individuals with the H4H4 haplotype combination;

[0045] Individuals with the H3H3, H3H4, or H3H6 haplotype combinations had significantly lower chest circumferences than individuals with the H4H4 or H4H5 haplotype combinations.

[0046] The tube circumference of individuals with the H3H3 haplotype combination was significantly smaller than that of individuals with the H3H4 haplotype combination, and the tube circumference of individuals with the H3H3 haplotype combination was significantly smaller than that of individuals with the H4H4 haplotype combination.

[0047] As one possible approach, the reagent is a primer with a nucleotide sequence as shown in SEQ ID NO: 1-2.

[0048] Preferably, the breeding is molecular-assisted breeding of body size traits.

[0049] A method for breeding Leizhou goats, detecting the above-mentioned SNP molecular markers;

[0050] Individuals with the SNP1 locus genotype TT had significantly higher chest height, chest width, or chest circumference than individuals with the genotype CT or CC. Individuals with the genotype TT had significantly higher chest depth than individuals with the genotype CT. Individuals with the genotype TT had significantly higher canal circumference than individuals with the genotype CC.

[0051] Individuals with the SNP2 locus genotype GG had significantly higher cross height, chest width, or chest circumference than individuals with the genotypes CG or CC. Individuals with the genotype GG had significantly higher chest depth than individuals with the genotype CG, and individuals with the genotype GG had significantly higher canal circumference than individuals with the genotype CC.

[0052] Individuals with the SNP3 locus genotype TT had significantly higher chest height, chest width, or chest circumference than individuals with the genotype CT or CC. Individuals with the genotype TT had significantly higher chest depth than individuals with the genotype CT. Individuals with the genotype TT had significantly higher canal circumference than individuals with the genotype CC.

[0053] Individuals with the SNP4 locus genotype GG had significantly higher cross height, chest width, or chest circumference than individuals with the genotype TG or TT. Individuals with the genotype GG had significantly higher chest depth than individuals with the genotype TG. Individuals with the genotype GG had significantly higher canal circumference than individuals with the genotype TT.

[0054] Individuals with the SNP5 genotype CC had significantly higher body height or cross height than individuals with the genotype TC. Individuals with the genotype CC had significantly higher chest depth, chest width, chest circumference, or cannon bone circumference than individuals with the genotype TC or TT.

[0055] Individuals with the SNP6 locus genotype AA had significantly higher body height or cross height than individuals with the genotype TA. Individuals with the genotype AA had significantly higher chest depth or cannon circumference than individuals with the genotype TA or TT. Individuals with the genotype AA had significantly higher chest circumference than individuals with the genotype TT.

[0056] Individuals with the SNP7 locus genotype AA had significantly higher cross height, chest width, or chest circumference than individuals with the genotype GA or GG; individuals with the genotype AA had significantly higher chest depth than individuals with the genotype GA; and individuals with the genotype AA had significantly higher canal circumference than individuals with the genotype GG.

[0057] Individuals with the SNP8 locus genotype TT had significantly higher cross height, chest width, or chest circumference than individuals with the genotype CT or CC. Individuals with the genotype TT had significantly higher chest depth than individuals with the genotype CT. Individuals with the genotype TT had significantly higher canal circumference than individuals with the genotype CC.

[0058] Individuals with the SNP9 genotype GG had significantly higher chest height, chest width, or chest circumference than individuals with the genotypes AG or AA. Individuals with the genotype GG had significantly higher chest depth than individuals with the genotype AG. Individuals with the genotype GG had significantly higher canal circumference than individuals with the genotype AA.

[0059] Preferably, the method includes the following steps:

[0060] S1. Extract genomic DNA from the Leizhou goats to be tested;

[0061] S2. The genomic DNA obtained in step S1 is amplified by PCR using reagents, and then the PCR amplification products are sequenced to obtain sequencing data;

[0062] S3. Analyze the sequencing data obtained in step S2 and determine the traits of the Leizhou goat to be tested based on SNP molecular markers.

[0063] A method for breeding Leizhou goats, detecting the above-mentioned haplotype SNP molecular markers;

[0064] Individuals with the H4 haplotype had significantly higher chest depth, chest circumference, or cannon circumference than individuals with the H3 haplotype.

[0065] The cross-shaped height of individuals in the H3H4 haplotype combination was significantly lower than that of individuals in the H4H4 haplotype combination;

[0066] Individuals with the H3H3, H3H4, or H3H6 haplotype combinations had significantly lower chest depths than individuals with the H4H4 haplotype combination.

[0067] Individuals with the H3H3 haplotype combination had significantly lower chest width than individuals with the H4H4 haplotype combination;

[0068] Individuals with the H3H3, H3H4, or H3H6 haplotype combinations had significantly lower chest circumferences than individuals with the H4H4 or H4H5 haplotype combinations.

[0069] The tube circumference of individuals with the H3H3 haplotype combination was significantly smaller than that of individuals with the H3H4 haplotype combination, and the tube circumference of individuals with the H3H3 haplotype combination was significantly smaller than that of individuals with the H4H4 haplotype combination.

[0070] Preferably, the method includes the following steps:

[0071] S1. Extract genomic DNA from the Leizhou goats to be tested;

[0072] S2. The genomic DNA obtained in step S1 is amplified by PCR using reagents, and then the PCR amplification products are sequenced to obtain sequencing data;

[0073] S3. Analyze the sequencing data obtained in step S2, and determine the traits of the Leizhou goat to be tested based on the combination of haplotype SNP molecular markers.

[0074] Preferably, in step S2, the sequencing is Sanger sequencing.

[0075] Preferably, in step S3, the characteristics are body height, cross height, chest depth, chest width, chest circumference and / or tube circumference.

[0076] Preferably, the breeding is molecular-assisted breeding of body size traits.

[0077] Compared with the prior art, the present invention has the following beneficial effects:

[0078] This invention provides molecular markers, detection reagents, methods, and applications for breeding Leizhou goats. CDK14 SNP sites and haplotype molecular markers significantly associated with body size traits in Leizhou goats were identified through screening exon 10 of the gene, which can be applied to early-stage auxiliary selection in Leizhou goat breeding. This invention also provides detection reagents and methods for detecting these SNP sites and haplotype molecular markers, enabling rapid, effective, and accurate prediction of body size traits in Leizhou goats, thereby saving production costs and accelerating the breeding process, and possessing significant economic and scientific research value. Attached Figure Description

[0079] Figure 1 This is a schematic diagram for measuring the body size characteristics of Leizhou goats, where: BH - body height, HH - cross height, CD - chest depth, CW - chest width, BOL - body length, CC - chest circumference, and CBC - cannon bone circumference.

[0080] Figure 2 Leizhou goat CDK14 Electrophoresis diagram of PCR amplification products of 10 exons of a gene.

[0081] Figure 3 Individual samples of Leizhou goats CDK14 Sequencing peak diagram of PCR product of exon 10 of gene.

[0082] Figure 4 The diagram consists of a linkage disequilibrium map of SNP loci and the percentage of haplotypes. In Figure A, the values ​​represent 100×D'; in Figure B, the values ​​represent 100×R². The haplotype loci are ordered from right to left. Detailed Implementation

[0083] The present invention will be further illustrated below with reference to specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in this technical field.

[0084] Unless otherwise specified, all reagents and materials used in the following examples are commercially available.

[0085] The example selected 97 healthy female Leizhou goats aged 1 to 4 years, all of which came from the Leizhou Goat Conservation Farm of Guangdong Ocean University in Zhanjiang City, Guangdong Province, China (geographical coordinates: 110°18′E, 21°10′N).

[0086] The body height (BH), body length (BOL), cross height (HH), chest width (CW), and chest depth (CD) of Leizhou goats were measured using a measuring stick, while the cannon circumference (CBC) and chest circumference (CC) were measured using a soft measuring tape. Figure 1 );

[0087] Body height (BH): The vertical distance from the highest point of the withers to the ground;

[0088] Hip cruciate height (HH): The vertical distance from the hip cruciate (ischial tuberosity level) to the ground;

[0089] Chest depth (CD): The vertical distance from the highest point of the withers to the lower edge of the sternum;

[0090] Chest width (CW): The straight-line distance between the widest points of the posterior borders of the two scapulae;

[0091] Chest circumference (CC): The length around the chest at the level of the posterior border of the shoulder blades;

[0092] CBC: The horizontal circumference of the narrowest part of the canal bone in the left forelimb;

[0093] Body diagonal length (BOL): also known as body diagonal length, refers to the distance from the anterior edge of the shoulder joint to the posterior edge of the ischial tuberosity.

[0094] Example 1: Leizhou Goat CDK14 Amplification of exon 10 of the gene

[0095] I. Experimental Methods

[0096] 1. Extraction and quality testing of blood DNA

[0097] Blood samples were collected from the jugular vein of goats using vacuum blood collection tubes containing disodium ethylenediaminetetraacetate (EDTA) as an anticoagulant. After inverting and mixing, the samples were temporarily stored at 0–4°C and then stored at -20°C after transportation. Subsequently, genomic DNA was extracted from the blood according to the instructions of the genomic DNA extraction kit. The quality of the extracted DNA was detected by 1% agarose gel electrophoresis (w / v) and a nucleic acid quantification instrument. Qualified DNA samples were stored at -20°C for later use.

[0098] 2. Primer design and synthesis

[0099] Referencing the NCBI database for goats CDK14 The full-length sequence information of the gene (Gene ID: 102176014; GeneBank: NC_030811.1) was used to design specific primers for exon 10 of this gene using Clone Manager V8 software. The primer sequences corresponding to the amplified sequence are shown in Table 1. The primers were synthesized by Suzhou Genewiz Biotechnology Co., Ltd.

[0100] Table 1 Leizhou Goat CDK14 Gene detection primers

[0101]

[0102] 3. PCR amplification

[0103] All DNA samples were diluted to 50 ng / μL and used as templates for PCR amplification. The PCR reaction system consisted of: 2 μL genomic DNA template, 5 μL 10× buffer, 5 μL dNTPs, 3 μL MgSO4, 1.5 μL each of forward and reverse primers, 1 μL high-fidelity enzyme, and 31 μL ddH2O.

[0104] PCR reaction conditions: 98℃ pre-denaturation for 2 min; 98℃ denaturation for 10 s, 68℃ annealing for 30 s (decreasing by 1℃ per cycle), 72℃ extension for 1 min, for a total of 10 cycles; 98℃ denaturation for 10 s, 58℃ annealing for 30 s, 72℃ extension for 1 min, for a total of 34 cycles; after all cycles, 72℃ extension for 5 min, then terminate the reaction.

[0105] Take an appropriate amount of PCR amplification product and mix it with nucleic acid loading buffer. After detection by 1% agarose gel electrophoresis (w / v), the product with the expected fragment size is directly sent to Sangon Biotech (Shanghai) Co., Ltd. for Sanger single-end sequencing (no purification required).

[0106] II. Experimental Results

[0107] Results of 1% agarose gel electrophoresis (w / v) are as follows: Figure 2 As shown, the amplified fragment length is 651 bp. The amplified target fragment band is clear, uncontaminated, and free of nonspecific bands. The fragment size is consistent with expectations.

[0108] Example 2: Leizhou Goat CDK14 SNP site detection of genes

[0109] I. Experimental Methods

[0110] The sequence peak diagrams in the sequencing results of Example 1 were compared using SnapGene 6.0.2 software to screen SNP sites, and the primers were individually tested.

[0111] The genotyping method is as follows: Open the Sanger sequencing file with SnapGene 6.0.2 software and directly observe the peak pattern: A single sharp peak at a specific nucleotide position indicates a homozygous genotype, while overlapping double peaks of similar intensity at the same position indicate a heterozygous genotype.

[0112] Sequencing results for each sample were screened, and single-base mutations were statistically analyzed. Only high-quality sequencing data with clear peak patterns were included to ensure genotyping accuracy. SNP information and the genotype of each sample were recorded using Excel.

[0113] II. Experimental Results

[0114] After comparing the sequencing results with the reference fragment on NCBI, SNP sites were screened, and a total of 9 SNP sites were found: g.112210991 C>T, g.112210978 C>G, g.112210954 C>T, g.112210869 T>G, g.112210845 T>C, g.112210753 T>A, g.112210669 G>A, g.112210653 C>T, g.112210652 A>G ( Figure 3 ).

[0115] Example 3: Leizhou Goat CDK14 Genetic parameter analysis of gene SNP loci

[0116] I. Experimental Methods

[0117] The gene frequency, genotype frequency, genetic heterozygosity, homozygosity, chi-square value, and Hardy-Weinberg equilibrium P-value of each SNP locus in Example 2 were calculated using Excel. The specific calculation formulas are as follows:

[0118]

[0119] Where n is the number of alleles at a certain locus; Pi and Pj are the gene frequencies of the i-th and j-th alleles in the population, respectively.

[0120] II. Experimental Results

[0121] CDK14Table 2 shows the genotype frequencies, allele frequencies, and population genetic parameters of the nine SNP loci in exon 10. The polymorphism information content (PIC) of all loci ranged from 0.25 to 0.5, indicating moderate polymorphism. The Hardy-Weinberg equilibrium (HWE) test showed that the P-values ​​for all nine SNP loci were greater than 0.05, indicating that the genotype distribution of these nine loci conformed to genetic equilibrium and followed Mendelian inheritance laws. The selected population was a randomly sampled population. Further analysis of heterozygosity (He) and homozygosity (Ho) revealed that the He values ​​for all nine loci were close to 0.500. The homozygosity of the g.112210845T>C and g.112210753T>A loci was slightly higher, indicating a relatively balanced allele distribution at these nine loci. The allele frequencies before and after the mutation were similar in the population, and there was no obvious dominant genotype.

[0122] Table 2 Leizhou Goat CDK14 Population genetic parameters of SNP loci in exon 10 of a gene

[0123]

[0124] Example 4 CDK14 Association analysis between gene SNP loci and body size traits in Leizhou goats

[0125] I. Experimental Methods

[0126] IBM SPSS Statistics 27.0.1 software was used to analyze the data using one-way ANOVA and LSD multiple comparisons. CDK14 The association between genotypes at different SNP loci in exon 10 of a gene and body size traits was studied. Experimental data are expressed as mean ± standard deviation.

[0127] II. Experimental Results

[0128] The results are shown in Table 3. At the nine SNP loci in exon 10, the rare homozygous individuals had higher average body size traits (except for height) than the dominant homozygous and heterozygous individuals. Specifically, the average body size traits of seven loci (g.112210991 C>T, g.112210978 C>G, g.112210954 C>T, g.112210869 T>G, g.112210669 G>A, g.112210653 C>T, g.112210652 A>G) were completely consistent, and the rare homozygous individuals (TT, GG, TT, GG, AA, TT, GG) had higher measurements for all body size traits.

[0129] Regarding chest depth traits, the rare homozygous types (TT, GG, TT, GG, AA, TT, GG) at these 7 loci were significantly higher than the heterozygous types (CT, CG, CT, TG, GA, CT, AG) (P<0.05). There were no significant differences between the dominant homozygous types (CC, CC, CC, TT, GG, CC, AA) and the rare homozygous types (TT, GG, TT, GG, AA, TT, GG) and heterozygous types (CT, CG, CT, TG, GA, CT, AG) (P>0.05).

[0130] In terms of cross height, chest width, and chest circumference, rare homozygous types (TT, GG, TT, GG, AA, TT, GG) were significantly higher than heterozygous types (CT, CG, CT, TG, GA, CT, AG) and dominant homozygous types (CC, CC, CC, TT, GG, CC, AA) (P<0.05). There was no significant difference between heterozygous types (CT, CG, CT, TG, GA, CT, AG) and dominant homozygous types (CC, CC, CC, TT, GG, CC, AA) (P>0.05).

[0131] Regarding the perivascular traits, rare homozygous types (TT, GG, TT, GG, AA, TT, GG) were significantly higher than dominant homozygous types (CC, CC, CC, TT, GG, CC, AA) (P<0.05), while there were no significant differences between heterozygous types (CT, CG, CT, TG, GA, CT, AG) and rare homozygous types (TT, GG, TT, GG, AA, TT, GG) and dominant homozygous types (CC, CC, CC, TT, GG, CC, AA) (P>0.05).

[0132] The rare homozygous CC at the g.112210845 T>C site showed higher values ​​for all body size traits:

[0133] In terms of body height and cruciate height, the rare homozygous CC was significantly higher than the heterozygous TC (P<0.05), while there was no significant difference between the dominant homozygous TT and the rare homozygous CC and heterozygous TC (P>0.05).

[0134] In terms of chest depth, chest width, chest circumference, and canal circumference, the rare homozygous CC was significantly higher than that of the heterozygous TC and the dominant homozygous TT (P<0.05), while there was no significant difference between the dominant homozygous TT and the heterozygous TC (P>0.05).

[0135] The rare homozygous AA at the g.112210753 T>A site had higher measurements across all body size traits:

[0136] In terms of chest depth and canal circumference, the rare homozygous AA was significantly higher than the heterozygous TA and the dominant homozygous TT (P<0.05), while there was no significant difference between the heterozygous TA and the dominant homozygous TT (P>0.05).

[0137] In terms of body height and cruciate height, the rare homozygous AA was significantly higher than the heterozygous TA (P<0.05), while there was no significant difference between the dominant homozygous TT and the rare homozygous AA and heterozygous TA (P>0.05).

[0138] In terms of chest circumference, the rare homozygous AA was significantly higher than the dominant homozygous TT (P<0.05), while there was no significant difference between the heterozygous TA and the rare homozygous AA and dominant homozygous TT (P>0.05).

[0139] Table 3. Association analysis of different SNP loci at exon 10 of the CDK14 gene in Leizhou goats with body size traits.

[0140]

[0141] Note: Different letters in the same column indicate differences in body size traits among different genotypes at the same locus. "a" and "b" indicate significant differences (P<0.05), while the same letter or no letter indicates no significant differences (P>0.05).

[0142] Example 5: Leizhou Goat CDK14 Gene linkage disequilibrium and SNP haplotype analysis

[0143] I. Experimental Methods

[0144] Linkage disequilibrium (LD) analysis was performed using Haploview 4.2 software to assess linkage relationships between SNP sites, define haplotype blocks, and derive haplotypes.

[0145] II. Experimental Results

[0146] The results are as follows Figure 4 As shown in A and B, a strong linkage relationship (RT) exists among the following 9 loci: g.112210991 C>T, g.112210978 C>G, g.112210954 C>T, g.112210869 T>G, g.112210845 T>C, g.112210753 T>A, g.112210669 G>A, g.112210653 C>T, and g.112210652 A>G. 2>0.33, D'= 1), among which the seven sites g.112210652 A>G, g.112210653 C>T, g.112210669 G>A, g.112210869 T>G, g.112210954 C>T, g.112210978 C>G, and g.112210991 C>T are fully linked (D'=1, R 2 = 1). These 9 sites form haplotype block 1, from which 4 different haplotypes were obtained: CCCTTGCA (H3), TGTGCAATG (H4), TGTGTTATG (H5), and TGTGCTATG (H6), with haplotype frequencies of 0.505, 0.351, 0.088, and 0.057, respectively. Figure 4 ).

[0147] Example 6: Leizhou Goat CDK14 Association analysis between gene SNP haplotypes and body size traits

[0148] I. Experimental Methods

[0149] IBM SPSS Statistics 27.0.1 software was used to analyze the data using one-way ANOVA and LSD multiple comparisons. CDK14 The association between haplotypes at different SNP sites in exon 10 of a gene and body size traits was studied. Experimental data are expressed as mean ± standard deviation.

[0150] II. Experimental Results

[0151] After excluding haplotypes with fewer than 3 individuals, association analysis was performed on the two haplotype blocks formed by the nine loci and body size traits. The results shown in Table 4 indicate that the two haplotypes H3 and H4 in the haplotype region showed significant differences in chest depth, chest circumference, and cannon circumference traits (P<0.05).

[0152] Table 4 CDK14 Association between haplotypes of exon 10 amplification region and body size traits in Leizhou goats

[0153]

[0154] Note: Haplotype combinations with fewer than 3 individuals were not statistically analyzed.

[0155] Example 7: Leizhou Goat CDK14 Association analysis between gene SNP haplotype combinations and body size traits

[0156] I. Experimental Methods

[0157] IBM SPSS Statistics 27.0.1 software was used to analyze the data using one-way ANOVA and LSD multiple comparisons. CDK14 The association between haplotype combinations of different SNP sites in exon 10 of a gene and body size traits was studied. Experimental data are expressed as mean ± standard deviation.

[0158] II. Experimental Results

[0159] Within the haplotype regions (Table 5), there were significant intragroup differences in chest circumference and tube girth among different haplotype combinations (P<0.05): the average chest circumference of the H4H5 haplotype combination was greater than that of the other 5 combinations, and there was a significant difference compared with the H3H3, H3H4, and H3H6 combinations (P<0.05); the average tube girth of the H4H4 haplotype combination was also greater than that of the other 5 combinations, and there was a significant difference compared with the H3H3 and H3H4 combinations (P<0.05).

[0160] In terms of the high cross-shaped trait, the H4H4 haplotype combination was significantly different from the H3H4 combination (P<0.05), but not significantly different from other haplotype combinations (P>0.05).

[0161] Regarding chest depth, the H4H4 haplotype combination showed significant differences only with some haplotype combinations (P<0.05). Specifically, H4H4 differed significantly from the H3H3, H3H4, and H3H6 combinations in chest depth (P<0.05), but showed no significant differences from the other combinations (P>0.05).

[0162] Regarding chest width, the H4H4 haplotype combination showed significant differences only with specific haplotype combinations (P<0.05), with a significant difference in chest width compared to the H3H3 combination (P<0.05).

[0163] Table 5 CDK14 Association between haplotype combinations of exon 10 amplification region and body size traits in Leizhou goats

[0164]

[0165] Note: Different letters in the same column indicate differences in body size traits among different genotypes at the same locus; a, b, and c indicate significant differences (P<0.05); the same letter or no letter indicates no significant differences (P>0.05); haplotype combinations with fewer than 3 individuals were not statistically analyzed.

[0166] Example 8: A method for breeding Leizhou goats

[0167] Single nucleotide polymorphism sites:

[0168] SNP1 is located at position 112210991 on NC_030811.1 and is a C or T polymorphism, with three genotypes: CC, CT, and TT.

[0169] SNP2 is located at position 112210978 on NC_030811.1 and is a C or G polymorphism, with three genotypes: CC, CG, and GG.

[0170] SNP3 is located at position 112210954 on NC_030811.1 and is a C or T polymorphism, with three genotypes: CC, CT, and TT.

[0171] SNP4 is located at position 112210869 on NC_030811.1 and is a T or G polymorphism, with three genotypes: TT, TG, and GG.

[0172] SNP5 is located at position 112210845 on NC_030811.1 and is a T or C polymorphism, with three genotypes: TT, TC, and CC.

[0173] SNP6 is located at position 112210753 on NC_030811.1 and is a T or A polymorphism, with three genotypes: TT, TA, and AA.

[0174] SNP7 is located at position 112210669 on NC_030811.1 and is a G or A polymorphism, with three genotypes: GG, GA, and AA.

[0175] SNP8 is located at position 112210653 on NC_030811.1 and is a C or T polymorphism, with three genotypes: CC, CT, and TT.

[0176] SNP9 is located at position 112210652 on NC_030811.1 and is an A or G polymorphism, with three genotypes: AA, AG, and GG.

[0177] 1. Extract DNA from the sample to be tested using a blood genomic DNA extraction kit.

[0178] 2. Using the DNA extracted from the sample in step 1 as a template, perform PCR amplification of SNP1 to SNP9 single nucleotide polymorphism sites;

[0179] The PCR reaction system consisted of: 2 μL DNA template, 5 μL 10× buffer, 5 μL dNTPs, 3 μL MgSO4, 1.5 μL each of primers with nucleotide sequences as shown in SEQ ID NO: 1-2, 1 μL high-fidelity enzyme, and 31 μL ddH2O.

[0180] PCR reaction conditions: 98℃ pre-denaturation for 2 min; 98℃ denaturation for 10 s, 68℃ annealing for 30 s (decreasing by 1℃ per cycle), 72℃ extension for 1 min, for a total of 10 cycles; 98℃ denaturation for 10 s, 58℃ annealing for 30 s, 72℃ extension for 1 min, for a total of 34 cycles; after all cycles, 72℃ extension for 5 min, then terminate the reaction.

[0181] 3. Perform agarose gel electrophoresis on the PCR amplification products obtained in step 2. If the electrophoretic bands are the same size as the target bands and are clear and bright, then sequence the PCR amplification products obtained in step 2.

[0182] 4. Result Interpretation:

[0183] It can detect single nucleotide polymorphism sites at SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNP8 and / or SNP9, among which:

[0184] Individuals with the SNP1 locus genotype TT had significantly higher chest height, chest width, or chest circumference than individuals with the genotype CT or CC. Individuals with the genotype TT had significantly higher chest depth than individuals with the genotype CT. Individuals with the genotype TT had significantly higher canal circumference than individuals with the genotype CC.

[0185] Individuals with the SNP2 locus genotype GG had significantly higher cross height, chest width, or chest circumference than individuals with the genotypes CG or CC. Individuals with the genotype GG had significantly higher chest depth than individuals with the genotype CG, and individuals with the genotype GG had significantly higher canal circumference than individuals with the genotype CC.

[0186] Individuals with the SNP3 locus genotype TT had significantly higher chest height, chest width, or chest circumference than individuals with the genotype CT or CC. Individuals with the genotype TT had significantly higher chest depth than individuals with the genotype CT. Individuals with the genotype TT had significantly higher canal circumference than individuals with the genotype CC.

[0187] Individuals with the SNP4 locus genotype GG had significantly higher cross height, chest width, or chest circumference than individuals with the genotype TG or TT. Individuals with the genotype GG had significantly higher chest depth than individuals with the genotype TG. Individuals with the genotype GG had significantly higher canal circumference than individuals with the genotype TT.

[0188] Individuals with the SNP5 genotype CC had significantly higher body height or cross height than individuals with the genotype TC. Individuals with the genotype CC had significantly higher chest depth, chest width, chest circumference, or cannon bone circumference than individuals with the genotype TC or TT.

[0189] Individuals with the SNP6 locus genotype AA had significantly higher body height or cross height than individuals with the genotype TA. Individuals with the genotype AA had significantly higher chest depth or cannon circumference than individuals with the genotype TA or TT. Individuals with the genotype AA had significantly higher chest circumference than individuals with the genotype TT.

[0190] Individuals with the SNP7 locus genotype AA had significantly higher cross height, chest width, or chest circumference than individuals with the genotype GA or GG; individuals with the genotype AA had significantly higher chest depth than individuals with the genotype GA; and individuals with the genotype AA had significantly higher canal circumference than individuals with the genotype GG.

[0191] Individuals with the SNP8 locus genotype TT had significantly higher cross height, chest width, or chest circumference than individuals with the genotype CT or CC. Individuals with the genotype TT had significantly higher chest depth than individuals with the genotype CT. Individuals with the genotype TT had significantly higher canal circumference than individuals with the genotype CC.

[0192] Individuals with the SNP9 genotype GG had significantly higher chest height, chest width, or chest circumference than individuals with the genotypes AG or AA. Individuals with the genotype GG had significantly higher chest depth than individuals with the genotype AG. Individuals with the genotype GG had significantly higher canal circumference than individuals with the genotype AA.

[0193] Alternatively, it can detect haplotypes or combinations of haplotypes composed of single nucleotide polymorphisms (SNPs) at SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNP8, and SNP9:

[0194] SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNP8, and SNP9 construct four haplotypes:

[0195] The H3 haplotype is CCCTTGCA, the H4 haplotype is TGTGCAATG, the H5 haplotype is TGTGTTATG, and the H6 haplotype is TGTGCTATG.

[0196] Individuals with the H4 haplotype had significantly higher chest depth, chest circumference, or cannon circumference than individuals with the H3 haplotype.

[0197] The haplotypes construct haplotype combinations, which include the H3H3 haplotype combination, the H3H4 haplotype combination, the H3H6 haplotype combination, the H4H4 haplotype combination, and the H4H5 haplotype combination.

[0198] The cross-shaped height of individuals in the H3H4 haplotype combination was significantly lower than that of individuals in the H4H4 haplotype combination;

[0199] Individuals with the H3H3, H3H4, or H3H6 haplotype combinations had significantly lower chest depths than individuals with the H4H4 haplotype combination.

[0200] Individuals with the H3H3 haplotype combination had significantly lower chest width than individuals with the H4H4 haplotype combination;

[0201] Individuals with the H3H3, H3H4, or H3H6 haplotype combinations had significantly lower chest circumferences than individuals with the H4H4 or H4H5 haplotype combinations.

[0202] The tube circumference of individuals with the H3H3 haplotype combination was significantly smaller than that of individuals with the H3H4 haplotype combination, and the tube circumference of individuals with the H3H3 haplotype combination was significantly smaller than that of individuals with the H4H4 haplotype combination.

[0203] Example 9: A kit for breeding Leizhou goats

[0204] I. Composition

[0205] Primers with nucleotide sequences as shown in SEQ ID NO: 1-2, PCR amplification reagents, and ddH2O.

[0206] II. Instructions for Use

[0207] The detection and result interpretation were carried out in accordance with Example 8.

[0208] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. The use of reagents for detecting SNP molecular markers for Leizhou goat breeding in the preparation of Leizhou goat breeding products, characterized in that, The SNP molecular marker is selected from SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNP8 and / or SNP9; SNP1 is located at position 112210991 on NC_030811.1, which is a C or T polymorphism, and exists in three genotypes of CC, CT and TT; SNP2 is located at position 112210978 on NC_030811.1, which is a C or G polymorphism, and exists in three genotypes of CC, CG and GG; SNP3 is located at position 112210954 on NC_030811.1, which is a C or T polymorphism, and exists in three genotypes of CC, CT and TT; SNP4 is located at position 112210869 on NC_030811.1, which is a T or G polymorphism, and exists in three genotypes of TT, TG and GG; SNP5 is located at position 112210845 on NC_030811.1, which is a T or C polymorphism, and exists in three genotypes of TT, TC and CC; SNP6 is located at position 112210753 on NC_030811.1, which is a T or A polymorphism, and exists in three genotypes of TT, TA and AA; SNP7 is located at position 112210669 on NC_030811.1, which is a G or A polymorphism, and exists in three genotypes of GG, GA and AA; SNP8 is located at position 112210653 on NC_030811.1, which is a C or T polymorphism, and exists in three genotypes of CC, CT and TT; SNP9 is located at position 112210652 on NC_030811.1, which is a A or G polymorphism, and exists in three genotypes of AA, AG and GG; The individual with genotype TT of the SNP1 site has significantly higher cross section height, chest width or chest circumference than the individual with genotype CT or CC, the individual with genotype TT has significantly higher chest depth than the individual with genotype CT, and the individual with genotype TT has significantly higher pipe circumference than the individual with genotype CC; The individual with genotype GG of the SNP2 site has significantly higher cross section height, chest width or chest circumference than the individual with genotype CG or CC, the individual with genotype GG has significantly higher chest depth than the individual with genotype CG, and the individual with genotype GG has significantly higher pipe circumference than the individual with genotype CC; The individual with genotype TT of the SNP3 site has significantly higher cross section height, chest width or chest circumference than the individual with genotype CT or CC, the individual with genotype TT has significantly higher chest depth than the individual with genotype CT, and the individual with genotype TT has significantly higher pipe circumference than the individual with genotype CC; The individual with genotype GG of the SNP4 site has significantly higher cross section height, chest width or chest circumference than the individual with genotype TG or TT, the individual with genotype GG has significantly higher chest depth than the individual with genotype TG, and the individual with genotype GG has significantly higher pipe circumference than the individual with genotype TT; The individual with genotype GG of the SNP4 site has significantly higher cross section height, chest width or chest circumference than the individual with genotype TG or TT, the individual with genotype GG has significantly higher chest depth than the individual with genotype TG, and the individual with genotype GG has significantly higher pipe circumference than the individual with genotype TT; The individual with genotype CC of the SNP5 site has significantly higher body height or cross height than the individual with genotype TC, and the individual with genotype CC has significantly higher chest depth, chest width, chest circumference or girth than the individual with genotype TC or TT; The individual with genotype AA of the SNP6 site has significantly higher body height or cross height than the individual with genotype TA, and the individual with genotype AA has significantly higher chest depth or girth than the individual with genotype TA or TT, and the individual with genotype AA has significantly higher chest circumference than the individual with genotype TT; The individual with genotype AA of the SNP7 site has significantly higher cross height, chest width or chest circumference than the individual with genotype GA or GG, and the individual with genotype AA has significantly higher chest depth than the individual with genotype GA, and the individual with genotype AA has significantly higher girth than the individual with genotype GG; The individual with genotype TT of the SNP8 site has significantly higher cross height, chest width or chest circumference than the individual with genotype CT or CC, and the individual with genotype TT has significantly higher chest depth than the individual with genotype CT, and the individual with genotype TT has significantly higher girth than the individual with genotype CC; The individual with genotype GG of the SNP9 site has significantly higher cross height, chest width or chest circumference than the individual with genotype AG or AA, and the individual with genotype GG has significantly higher chest depth than the individual with genotype AG, and the individual with genotype GG has significantly higher girth than the individual with genotype AA.

2. The use of the reagent for detecting the haplotype SNP molecular marker for Leizhou goat breeding in the preparation of products for Leizhou goat breeding, characterized in that, The haplotype SNP molecular marker is constructed by 9 single nucleotide polymorphism sites SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNP8 and SNP9; SNP1 is located at position 112210991 of NC_030811.1, which is C or T polymorphism, and there are three genotypes of CC, CT and TT; SNP2 is located at position 112210978 of NC_030811.1, which is C or G polymorphism, and there are three genotypes of CC, CG and GG; SNP3 is located at position 112210954 of NC_030811.1, which is C or T polymorphism, and there are three genotypes of CC, CT and TT; SNP4 is located at position 112210869 of NC_030811.1, which is T or G polymorphism, and there are three genotypes of TT, TG and GG; SNP5 is located at position 112210845 of NC_030811.1, which is T or C polymorphism, and there are three genotypes of TT, TC and CC; SNP6 is located at position 112210753 of NC_030811.1, which is T or A polymorphism, and there are three genotypes of TT, TA and AA; SNP7 is located at position 112210669 of NC_030811.1, which is G or A polymorphism, and there are three genotypes of GG, GA and AA; SNP8 is located at position 112210653 of NC_030811.1, which is C or T polymorphism, and there are three genotypes of CC, CT and TT; SNP9 is located at position 112210652 on NC_030811.1, and is A or G polymorphism, and there are three genotypes of AA, AG and GG; The SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNP8 and SNP9 construct four haplotypes: The H3 haplotype is CCCTTTGCA, the H4 haplotype is TGTGCAATG, the H5 haplotype is TGTGTTATG, and the H6 haplotype is TGTGCTATG; The chest depth, chest circumference or girth of the individual with the H4 haplotype is significantly higher than that of the individual with the H3 haplotype; The haplotypes construct haplotype combinations, and the haplotype combinations include an H3H3 haplotype combination, an H3H4 haplotype combination, an H3H6 haplotype combination, an H4H4 haplotype combination and an H4H5 haplotype combination; The individual with the H3H4 haplotype combination has a significantly lower cross section than the individual with the H4H4 haplotype combination; The individual with the H3H3, H3H4 or H3H6 haplotype combination has a significantly lower chest depth than the individual with the H4H4 haplotype combination; The individual with the H3H3 haplotype combination has a significantly lower chest width than the individual with the H4H4 haplotype combination; The individual with the H3H3, H3H4 or H3H6 haplotype combination has a significantly lower chest circumference than the individual with the H4H4 or H4H5 haplotype combination; The individual with the H3H3 haplotype combination has a significantly lower girth than the individual with the H3H4 haplotype combination, and the individual with the H3H3 haplotype combination has a significantly lower girth than the individual with the H4H4 haplotype combination.

3. Use according to claim 1 or 2, characterized in that, The reagent is a primer with a nucleotide sequence as shown in SEQ ID NO: 1-2.

4. A method for breeding Leizhou goats, characterized in that, The SNP molecular marker described in claim 1 is detected; The individual with the genotype TT of the SNP1 site has a significantly higher cross section, chest width or chest circumference than the individual with the genotype CT or CC, the individual with the genotype TT has a significantly higher chest depth than the individual with the genotype CT, and the individual with the genotype TT has a significantly higher girth than the individual with the genotype CC; The individual with the genotype GG of the SNP2 site has a significantly higher cross section, chest width or chest circumference than the individual with the genotype CG or CC, the individual with the genotype GG has a significantly higher chest depth than the individual with the genotype CG, and the individual with the genotype GG has a significantly higher girth than the individual with the genotype CC; The individual with the genotype TT of the SNP3 site has a significantly higher cross section, chest width or chest circumference than the individual with the genotype CT or CC, the individual with the genotype TT has a significantly higher chest depth than the individual with the genotype CT, and the individual with the genotype TT has a significantly higher girth than the individual with the genotype CC; The individual with the genotype GG of the SNP4 site has a significantly higher cross section, chest width or chest circumference than the individual with the genotype TG or TT, the individual with the genotype GG has a significantly higher chest depth than the individual with the genotype TG, and the individual with the genotype GG has a significantly higher girth than the individual with the genotype TT; The individual with the genotype GG of the SNP4 site has a significantly higher cross section, chest width or chest circumference than the individual with the genotype TG or TT, the individual with the genotype GG has a significantly higher chest depth than the individual with the genotype TG, and the individual with the genotype GG has a significantly higher girth than the individual with the genotype TT; The body height or cross height of the individual with the SNP5 site genotype CC is significantly higher than that of the individual with the genotype TC, and the chest depth, chest width, chest circumference or girth of the individual with the genotype CC is significantly higher than that of the individual with the genotype TC or TT; The body height or cross height of the individual with the SNP6 site genotype AA is significantly higher than that of the individual with the genotype TA, the chest depth or girth of the individual with the genotype AA is significantly higher than that of the individual with the genotype TA or TT, and the chest circumference of the individual with the genotype AA is significantly higher than that of the individual with the genotype TT; The cross height, chest width or chest circumference of the individual with the SNP7 site genotype AA is significantly higher than that of the individual with the genotype GA or GG, the chest depth of the individual with the genotype AA is significantly higher than that of the individual with the genotype GA, and the girth of the individual with the genotype AA is significantly higher than that of the individual with the genotype GG; The cross height, chest width or chest circumference of the individual with the SNP8 site genotype TT is significantly higher than that of the individual with the genotype CT or CC, the chest depth of the individual with the genotype TT is significantly higher than that of the individual with the genotype CT, and the girth of the individual with the genotype TT is significantly higher than that of the individual with the genotype CC; The cross height, chest width or chest circumference of the individual with the SNP9 site genotype GG is significantly higher than that of the individual with the genotype AG or AA, the chest depth of the individual with the genotype GG is significantly higher than that of the individual with the genotype AG, and the girth of the individual with the genotype GG is significantly higher than that of the individual with the genotype AA.

5. The method of claim 4, wherein, The method comprises the following steps: S1. Extracting the genomic DNA of the Leizhou goat to be tested; S2. performing PCR amplification on the genomic DNA obtained in step S1 using reagents, and then performing sequencing on the PCR amplification product to obtain sequencing data; S3. analyzing the sequencing data obtained in step S2, and determining the traits of the Leizhou goat to be tested according to the SNP molecular marker.

6. A method for breeding Leizhou goats, characterized in that, The haplotype SNP molecular marker in claim 2 is detected; The chest depth, chest circumference or girth of the individual with the H4 haplotype is significantly higher than that of the individual with the H3 haplotype; The cross height of the individual with the H3H4 haplotype combination is significantly lower than that of the individual with the H4H4 haplotype combination; The chest depth of the individual with the H3H3, H3H4 or H3H6 haplotype combination is significantly lower than that of the individual with the H4H4 haplotype combination; The chest width of the individual with the H3H3 haplotype combination is significantly lower than that of the individual with the H4H4 haplotype combination; The chest circumference of the individual with the H3H3, H3H4 or H3H6 haplotype combination is significantly lower than that of the individual with the H4H4 or H4H5 haplotype combination; The girth of the individual with the H3H3 haplotype combination is significantly lower than that of the individual with the H3H4 haplotype combination, and the girth of the individual with the H3H3 haplotype combination is significantly lower than that of the individual with the H4H4 haplotype combination.

7. The method of claim 6, wherein, The method comprises the following steps: S1. Extracting the genomic DNA of the Leizhou goat to be tested; S2. performing PCR amplification on the genomic DNA obtained in step S1 using reagents, and then performing sequencing on the PCR amplification product to obtain sequencing data; S3. Analyzing the sequencing data obtained in step S2, determining the traits of the Leizhou goat to be tested according to the haplotype SNP molecular marker combination.

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