Bacteriostatic liquid with function of tightening and repairing vaginal mucosa tissues and production process thereof

Abstract

The invention discloses antibacterial liquid with functions of tightening and repairing vaginal mucosa tissues and a production process thereof. The antibacterial liquid is prepared from the following components in parts by weight: 20-30 parts of sandalwood oil, 20-30 parts of grape seed oil, 20-30 parts of sea buckthorn seed oil, 5-10 parts of polygala tenuifolia, 5-10 parts of semen cuscutae, 5-10 parts of fructus evodiae, 1-5 parts of Chinese prickly ash, 0.1-0.3 part of chlorhexidine acetate and 0.5-1.5 parts of a stabilizer, wherein the stabilizer comprises tert-butyl hydroxy anisd and caprylyl glycol; the antibacterial liquid prepared from the raw materials has good stability, and can maintain effective antibacterial ability in a severe environment and a long-term storage scene.

Description

Technical Field

[0001] This application relates to the field of hygiene products technology, and in particular to an antibacterial solution with the function of tightening and repairing vaginal mucosal tissue and its manufacturing process. Background Technology

[0002] Currently, with the increasing sophistication of women's intimate health needs, products that combine vaginal mucosal tightening and repair with antibacterial functions have become a hot topic in market research and development. Existing technological approaches mainly revolve around the synergistic mechanism of "antibacterial-repair-tightening," such as using composite formulas of bioactive factors and natural plant extracts to target and disinfect pathogens, promote mucosal cell proliferation, and regenerate collagen fibers. Some products further combine nanotechnology or supercritical extraction processes to improve ingredient permeability and bioavailability. Meanwhile, dosage form innovation has become a key breakthrough; for example, ointment formulations maintain antibacterial activity by mimicking the vaginal pH environment, while gel formulations utilize water-soluble matrices to achieve rapid absorption and long-lasting sustained release.

[0003] However, significant shortcomings remain in terms of stability. Chemically synthesized ingredients are susceptible to environmental factors; high temperatures may cause changes in their molecular structure, leading to degradation of active ingredients or solution stratification, affecting the durability of their antibacterial efficacy. Natural plant extracts face the risk of oxidation; some components are prone to discoloration and inactivation upon exposure to light or oxygen, and complex components in plant extracts may precipitate due to pH fluctuations, affecting product appearance and user experience. While compound formulations can integrate the advantages of multiple ingredients, the compatibility issues between different substances are prominent. For example, metal ions and organic components may form complexes, causing turbidity and limiting the reliability of the product in long-term storage scenarios. Summary of the Invention

[0004] To address the issue of oxidative decomposition of antibacterial solutions during long-term storage or under harsh conditions, an antibacterial solution with the function of tightening and repairing vaginal mucosal tissue and its manufacturing process are provided.

[0005] The above-mentioned objective of this invention is achieved through the following technical solutions: An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue comprises the following components in parts by weight: 20-30 parts sandalwood oil; Grape seed oil 20-30; 20-30 parts sea buckthorn seed oil; 5-10 parts of Polygala tenuifolia; 5-10 parts of dodder seed; 5-10 parts of Evodia rutaecarpa; 1-5 parts Sichuan peppercorns; Chlorhexidine acetate 0.1–0.3 parts; Stabilizer 0.5–1.5 parts; Stabilizers include tert-butylhydroxyanisole and caprylyl glycol.

[0006] By adopting the above technical solutions, sandalwood oil, as one of the main components, contains α-santalol and β-santalol, which can directly destroy the bacterial cell membrane structure, especially showing significant inhibitory effects on common pathogenic bacteria such as Staphylococcus aureus and Escherichia coli; grape seed oil is rich in flavonoids and phenolic compounds, whose antioxidant capacity can scavenge free radicals, reduce bacterial metabolic activity, and inhibit inflammation. In addition, the proanthocyanidins and linoleic acid in grape seed oil have antioxidant and anti-inflammatory properties, which can reduce oxidative stress damage to mucous membranes, promote fibroblast proliferation, and accelerate tissue repair; the volatile oil components of sea buckthorn seed oil have a direct inhibitory effect on Candida albicans and Bacillus subtilis, and can accelerate wound healing; both Polygala tenuifolia and Cuscuta chinensis have antibacterial effects. The saponins in Polygala tenuifolia can stimulate mucosal secretion and improve the local microenvironment, while the alcohol extract of Cuscuta chinensis can enhance humoral immunity and improve local disease resistance. Evodia rutaecarpa and Zanthoxylum bungeanum, as natural antibacterial agents, contain alkaloids (such as evodiamine) that can reduce mucosal inflammation by inhibiting the release of inflammatory factors (such as TNF-α and IL-6). Chlorhexidine acetate binds to bacterial membrane phospholipids through its cationic charge, causing intracellular leakage, and is effective against both Gram-positive and Gram-negative bacteria, forming a durable protective layer on the mucosa. The stabilizers tert-butylhydroxyanisole (TBHHA) and caprylyl glycol work together to maintain the stability of the system. TBHHA blocks the chain reaction of oil oxidation through its phenolic structure, preventing component degradation. Caprylyl glycol has both moisturizing and antibacterial functions, maintaining humidity by binding water molecules through hydrogen bonds and interfering with microbial enzyme activity. TBHHA effectively delays the oxidative rancidity of oils and oil-containing formulations, while caprylyl glycol mainly disrupts the cell membrane structure of microorganisms. The combination of the two allows the product to effectively resist oxidative deterioration and inhibit the growth of various microorganisms. In summary, this achieves a rapid and stable antibacterial effect.

[0007] Optionally, 0.5 to 2 parts of phytic acid-modified cyclodextrin may be added.

[0008] By adopting the above technical solutions, phytic acid-modified cyclodextrin encapsulates oil-soluble components (such as terpenoids in sandalwood oil and sea buckthorn seed oil) through its hollow structure, forming a molecular-level capsule system, which slows down the release rate of active ingredients and enhances permeability. The hydrophilic nature of cyclodextrin on the outside and the hydrophobic nature on the inside can improve the dispersion of components on the mucous membrane, while phytic acid chelates metal ions, which can reduce oxidative catalytic reactions and extend the shelf life of the product. In summary, this makes the antibacterial effect more lasting and avoids the inactivation of oil components due to high temperature.

[0009] Optionally, the mass ratio of tert-butylhydroxyanisole to caprylyl glycol is 1:(1-4).

[0010] By adopting the above technical solution, when tert-butyl hydroxyanisole and octyl glycol are combined in a mass ratio of 1:(1-4), the free radical scavenging ability of tert-butyl hydroxyanisole and the moisturizing and antibacterial properties of octyl glycol produce a synergistic effect; tert-butyl hydroxyanisole has high antioxidant efficiency at low concentrations, while octyl glycol can form a molecular network to stabilize the oil phase system within this ratio range, preventing phase separation; ensuring the chemical stability and functional continuity of the product, and avoiding degradation of the effective ingredients due to oxidation.

[0011] Optionally, 0.5 to 2 parts of surfactant, namely sodium lauroyl glutamate, may be added.

[0012] By adopting the above technical solution, sodium lauroyl glutamate, as an amino acid surfactant, promotes the dispersion of hydrophobic components (such as oils) in the aqueous phase by reducing the surface tension of the liquid. Its mechanism is that the hydrophobic chains are embedded in the oil droplet interface and the hydrophilic groups are arranged outward to form micelles. In addition, its mild properties avoid damage to the mucous membrane, improve the uniformity of the formulation and the absorption efficiency, make the antibacterial components more likely to come into contact with pathogens, and enhance the immediate antibacterial effect.

[0013] Optionally, 5 to 10 parts of a humectant may be added, which may be one of propylene glycol, 1,3-propanediol, or anhydrous ethanol.

[0014] By adopting the above technical solutions, propylene glycol, 1,3-propanediol, or anhydrous ethanol are used as moisturizers. They form hydrogen bonds with water molecules through hydroxyl groups, thereby reducing the evaporation loss of mucosal moisture, maintaining a moist environment for the mucosa to accelerate repair, and improving the utilization of ingredients.

[0015] Optionally, 0.1 to 0.5 parts of pH buffer may be added, wherein the pH buffer is citric acid and sodium citrate.

[0016] By adopting the above technical solution, citric acid and sodium citrate form a buffer pair, which maintains the pH of the system in a weakly acidic range through the ionization balance of carboxyl and hydroxyl groups. This environment is close to the natural pH value of the mucous membrane, which can reduce irritation during use. The buffer resists pH fluctuations caused by raw materials or storage, avoiding the destruction of component activity by acidic environment or damage to the mucous membrane caused by alkaline environment; thus enhancing product safety and stability.

[0017] Optionally, the mass ratio of sodium citrate to citric acid is (4-6):1.

[0018] By adopting the above technical solution, this ratio is not only unfavorable to the growth of most bacteria, but more importantly, it is close to the normal pH of the mucous membrane, which can reduce irritation, maintain the balance of the microecology, and ensure that pH-sensitive ingredients such as chlorhexidine acetate are always in the most effective form and active state.

[0019] The second objective of this invention is achieved through the following technical solution: A manufacturing process for an antibacterial solution with tightening and repairing vaginal mucosal tissue function as described above, characterized by comprising the following steps: S1: Add sandalwood oil, grapeseed oil, and sea buckthorn seed oil to a mixing pot and stir to obtain phase A; S2: Grind Polygala tenuifolia, Cuscuta chinensis, Evodia rutaecarpa, and Zanthoxylum bungeanum into powder, sift and mix to obtain a mixed powder; S3: Add the mixed powder to phase A and mix to obtain phase B; S4: Add all components except sandalwood oil, grapeseed oil, sea buckthorn seed oil, polygala root, dodder seed, evodia fruit, and Sichuan pepper to phase B and stir.

[0020] By adopting the above technical solution, the process ensures the functionality of the components through stepwise mixing: first, the oil base (phase A) is stirred to achieve preliminary emulsification; then, the mixed powder of traditional Chinese medicine (sieved to increase the specific surface area) is added to phase A to fully dissolve the fat-soluble active ingredients (such as flavonoids and terpenes); finally, other components are added to prevent sensitive components (such as chlorhexidine acetate and tert-butylhydroxyanisole) from being deactivated by prolonged heating or shearing, thus preserving antibacterial activity.

[0021] In summary, this application has at least the following beneficial effects: (1) tert-butylhydroxyanisole blocks the chain reaction of lipid oxidation through its phenolic structure, preventing component degradation; (2) Caprylyl glycol has both moisturizing and antibacterial functions. It maintains the humidity of the mucous membrane by binding water molecules through hydrogen bonds and interferes with the activity of microbial enzymes to prevent the components from deteriorating. (3) The combination of tert-butylhydroxyanisole and caprylyl glycol enables the product to effectively resist oxidative deterioration and inhibit the growth of various microorganisms, thus synergistically maintaining the stability of the active ingredients. Detailed Implementation

[0022] raw material Sandalwood oil and grapeseed oil were both purchased from Jiangxi Hairui Natural Plant Co., Ltd. Sea buckthorn seed oil was purchased from Qinghai Kangpu Biotechnology Co., Ltd. Polygala tenuifolia, Cuscuta chinensis, Evodia rutaecarpa, and Zanthoxylum bungeanum were all purchased from Tongrentang Pharmacy; Chlorhexidine acetate was purchased from Xi'an Fanghao Chemical Co., Ltd. tert-Butylhydroxyanisole, purity >99.5wt%, purchased from Shanghai Jurui Industrial Co., Ltd. Octyl glycol, purity ≥99.0 wt%, purchased from Guangdong Hainuo Kewei Biopharmaceutical Co., Ltd.; Phytic acid-modified cyclodextrin was purchased from Xi'an Qiyue Biotechnology Co., Ltd. Sodium lauroyl glutamate, purity ≥95wt%, purchased from Shanghai Maclean Biochemical Technology Co., Ltd. Sorbitol oleate, with a viscosity of 1000-2000 mPa·s (20℃), was purchased from Shanghai Maclean Biochemical Technology Co., Ltd. Potassium cocoyl glycinate, purity ≥95wt%, purchased from Shanghai Maclean Biochemical Technology Co., Ltd. Propylene glycol, purity ≥99.9wt%, was purchased from Shanghai Maclean Biochemical Technology Co., Ltd. 1,3-Propanediol, purity ≥99.0wt%, purchased from Shanghai Maclean Biochemical Technology Co., Ltd. Citric acid, sodium citrate, and anhydrous ethanol are all commercially available.

[0023] Preparation Example 1 A pH buffer is prepared as follows: 4g of citric acid and 1g of sodium citrate are weighed and added to 1L of deionized water, and mixed to obtain the pH buffer.

[0024] Preparation Example 2 A pH buffer, which differs from Preparation Example 1 in that: citric acid is 3g, and the rest is the same as Preparation Example 1.

[0025] Preparation Example 3 A pH buffer, which differs from Preparation Example 1 in that: citric acid is 5g, and the rest is the same as Preparation Example 1.

[0026] Preparation Example 4 A pH buffer, which differs from Preparation Example 1 in that: citric acid is 2g, and the rest is the same as Preparation Example 1.

[0027] Preparation Example 5 A pH buffer, which differs from Preparation Example 1 in that: 6g of citric acid is used, while the rest is the same as in Preparation Example 1.

[0028] Example 1 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is prepared from the following raw material components: sandalwood oil, grape seed oil, sea buckthorn seed oil, polygala root, dodder seed, evodia fruit, Sichuan pepper, chlorhexidine acetate, tert-butylhydroxyanisole, caprylyl glycol, phytic acid modified cyclodextrin, sodium lauroyl glutamate, propylene glycol, and pH buffer, wherein the pH buffer is derived from preparation example 1.

[0029] Its preparation process is as follows: S1: Add 250g sandalwood oil, 250g grapeseed oil and 250g sea buckthorn seed oil to a mixing pot in sequence, and stir at 50 rpm for 5 minutes to obtain phase A; S2: Grind 1kg of Polygala tenuifolia, 1kg of Cuscuta chinensis, 1kg of Evodia rutaecarpa, and 1kg of Zanthoxylum bungeanum into a grinder and pass through a 100-mesh sieve to obtain Polygala tenuifolia powder, Cuscuta chinensis powder, Evodia rutaecarpa powder, and Zanthoxylum bungeanum powder. Weigh out 70g of Polygala tenuifolia powder, 70g of Cuscuta chinensis powder, 70g of Evodia rutaecarpa powder, and 30g of Zanthoxylum bungeanum powder and mix them together to obtain a mixed powder. S3: Add the mixed powder to phase A and mix at 800 rpm for 30 minutes to obtain phase B; S4: Add 2g chlorhexidine acetate, 2.5g tert-butylhydroxyanisole, 7.5g octyl glycol, 10g phytic acid modified cyclodextrin, 10g sodium lauroyl glutamate, 70g propylene glycol, and 3g pH buffer to phase B in sequence, and stir at 100 rpm for 10 min to obtain the antibacterial solution.

[0030] Comparative Example 1 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that: caprylyl glycol is not added, while the rest is the same as Example 1.

[0031] Comparative Example 2 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that it does not contain tert-butylhydroxyanisole, while the rest is the same as Example 1.

[0032] Example 2 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that: phytic acid-modified cyclodextrin is not added, while the rest is the same as Example 1.

[0033] Example 3 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from that in Example 1 in that: 5g of tert-butylhydroxyanisole and 5g of caprylyl glycol are used, while the rest are the same as in Example 1.

[0034] Example 4 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from that in Example 1 in that: 2g of tert-butylhydroxyanisole and 8g of caprylyl glycol are used, while the rest are the same as in Example 1.

[0035] Example 5 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from that in Example 1 in that: 6g of tert-butylhydroxyanisole and 4g of caprylyl glycol are used, while the rest are the same as in Example 1.

[0036] Example 6 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from that in Example 1 in that: 1g of tert-butylhydroxyanisole and 9g of caprylyl glycol are used, while the rest are the same as in Example 1.

[0037] Example 7 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that: sodium lauroyl glutamate is not added, while the rest is the same as Example 1.

[0038] Example 8 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from that in Example 1 in that: sodium lauroyl glutamate is replaced with an amount of sorbitan oleate, while the rest is the same as in Example 1.

[0039] Example 9 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from that in Example 1 in that: potassium cocoyl glycinate is used instead of sodium lauroyl glutamate by mass, while the rest is the same as in Example 1.

[0040] Example 10 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that: propylene glycol is not added, while the rest is the same as Example 1.

[0041] Example 11 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that 1,3-propanediol is used instead of propylene glycol by mass, while the rest is the same as in Example 1.

[0042] Example 12 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that: anhydrous ethanol is used instead of propylene glycol by mass, while the rest is the same as in Example 1.

[0043] Example 13 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that: no pH buffer is added, and the rest is the same as Example 1.

[0044] Example 14 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that the pH buffer is derived from Preparation Example 2, and the rest is the same as Example 1.

[0045] Example 15 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that the pH buffer is derived from Preparation Example 3, and the rest is the same as Example 1.

[0046] Example 16 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that the pH buffer is derived from Preparation Example 4, and the rest is the same as Example 1.

[0047] Example 17 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from Example 1 in that the pH buffer is derived from Preparation Example 5, and the rest is the same as Example 1.

[0048] Example 18 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue is different from that in Example 1 in that: 200g of sandalwood oil, 200g of grape seed oil, 200g of sea buckthorn seed oil, 50g of Polygala tenuifolia, 50g of Cuscuta chinensis, 50g of Evodia rutaecarpa, 10g of Zanthoxylum bungeanum, 1g of chlorhexidine acetate, 1.25g of tert-butylhydroxyanisole, 3.75g of caprylyl glycol, 5g of phytic acid-modified cyclodextrin, 5g of sodium lauroyl glutamate, 50g of propylene glycol, and 1g of pH buffer are added to the antibacterial solution, while the rest are the same as in Example 1.

[0049] Example 19 An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue differs from Example 1 in that: the antibacterial solution contains 300g of sandalwood oil, 300g of grape seed oil, 300g of sea buckthorn seed oil, 100g of Polygala tenuifolia, 100g of Cuscuta chinensis, 100g of Evodia rutaecarpa, 50g of Zanthoxylum bungeanum, 3g of chlorhexidine acetate, 3.75g of tert-butylhydroxyanisole, 11.25g of caprylyl glycol, 20g of phytic acid-modified cyclodextrin, 20g of sodium lauroyl glutamate, 100g of propylene glycol, and 5g of pH buffer, while the remaining components are the same as in Example 1.

[0050] The antibacterial solutions obtained in Examples 1-19 and Comparative Examples 1-2 were tested.

[0051] Examples 1-19 and Comparative Examples 1-2 were tested according to Appendix E of GB 15979-2024 "Hygienic Requirements for Disposable Sanitary Products" for the test methods of bactericidal performance, bacteriostatic performance and stability. The test bacteria were Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923), and the culture medium was potato dextrose agar. The bacteriostatic performance was tested by the antibacterial agent bactericidal performance test, and the test results are shown in Table 1. The stability was tested by the accelerated test (chemical determination method, stored at 54℃ for 14 days), and the test results are shown in Table 1.

[0052] Table 1 Results of antibacterial ability and stability tests Compared with Comparative Examples 1 and 2, the antibacterial rates of Staphylococcus aureus and Escherichia coli in Example 1 were greater than those in Comparative Examples 1 and 2, while the degradation rates of the active ingredients in Example 1 were less than those in Comparative Examples 1 and 2.

[0053] The difference between Example 1 and Comparative Examples 1 and 2 is that in Example 1, both tert-butylhydroxyanisole and caprylyl glycol were added. Both tert-butylhydroxyanisole and caprylyl glycol maintained the stability of the system. Tert-butylhydroxyanisole blocked the chain reaction of oil oxidation and prevented the degradation of components. Caprylyl glycol has both moisturizing and antibacterial functions. It maintains the humidity of the mucous membrane by binding water molecules through hydrogen bonds and interferes with the activity of microbial enzymes. It can be seen that the addition of both tert-butylhydroxyanisole and caprylyl glycol is necessary.

[0054] Comparing Example 1 and Example 2, the inhibition rates of Staphylococcus aureus and Escherichia coli in Example 1 were greater than those in Example 2, while the degradation rates of the active ingredients in Example 1 were less than those in Example 2.

[0055] The difference between Example 1 and Example 2 is that Example 1 added phytic acid-modified cyclodextrin. Phytic acid-modified cyclodextrin encapsulates oil-soluble components through its cavity structure, forming a molecular-level capsule system, which slows down the release rate of active ingredients and enhances permeability. Chelating metal ions can reduce oxidative catalytic reactions and extend the shelf life of the product. Furthermore, the hydrophilic nature of cyclodextrin on the outside and the hydrophobic nature on the inside can improve the dispersion of components on the mucous membrane. It can be seen that the addition of phytic acid-modified cyclodextrin is superior.

[0056] Compared with Examples 1 and Examples 3-6, the inhibition rates of Staphylococcus aureus and Escherichia coli in Example 1 were greater than those in Examples 3-6, and the degradation rates of the active ingredients in Example 1 were less than those in Examples 3-6. The inhibition rates of Staphylococcus aureus and Escherichia coli in Examples 3-4 were greater than those in Examples 5-6, and the degradation rates of the active ingredients in Examples 3-4 were less than those in Examples 5-6.

[0057] The difference between Examples 1, 3-4 and Examples 5-6 is that the mass ratio of tert-butylhydroxyanisole to octyl glycol in Examples 1, 3-4 is 1:(1-4), and the mass ratio of tert-butylhydroxyanisole to octyl glycol in Example 1 is 1:3. Within this range, tert-butylhydroxyanisole has high antioxidant efficiency at low concentrations, while octyl glycol can form a molecular network to stabilize the oil phase system and prevent phase separation within this ratio range. It can be seen that a mass ratio of tert-butylhydroxyanisole to octyl glycol of 1:(1-4) is preferred.

[0058] Compared with Examples 1 and Examples 7-9, the inhibition rates of Staphylococcus aureus and Escherichia coli in Example 1 were greater than those in Examples 7-9, and the degradation rates of the active ingredients in Example 1 were less than those in Examples 7-9; the inhibition rates of Staphylococcus aureus and Escherichia coli in Examples 8-9 were greater than those in Example 7, and the degradation rates of the active ingredients in Examples 8-9 were less than those in Example 7.

[0059] The difference between Example 1 and Examples 7-9 is that: Examples 1 and 8-9 added surfactants, and the surfactant added in Example 1 was sodium lauroyl glutamate; sodium lauroyl glutamate promotes the dispersion of hydrophobic components in the aqueous phase by reducing the surface tension of the liquid, and its mild properties avoid damage to the mucous membrane, improve the uniformity and absorption efficiency of the formulation, make the antibacterial components more likely to contact pathogens, and enhance the immediate antibacterial effect; it can be seen that adding a surfactant is better, and adding sodium lauroyl glutamate is better.

[0060] Compared with Examples 1 and 10-12, the inhibition rates of Staphylococcus aureus and Escherichia coli in Examples 1 and 11-12 were greater than those in Example 12, while the degradation rates of the active ingredients in Examples 1 and 11-12 were less than those in Example 12.

[0061] The difference between Example 1 and Examples 10-12 is that: in Examples 1 and 11-12, a humectant was added, and the humectant was propylene glycol, 1,3-propanediol or anhydrous ethanol; propylene glycol, 1,3-propanediol or anhydrous ethanol, as humectants, form hydrogen bonds with water molecules through hydroxyl groups, reducing the loss of moisture from mucous membrane evaporation; it can be seen that adding a humectant is better.

[0062] Comparing Example 1 and Example 13, the inhibition rates of Staphylococcus aureus and Escherichia coli in Example 1 were greater than those in Example 13, while the degradation rates of the active ingredients in Example 1 were less than those in Example 13.

[0063] The difference between Example 1 and Example 13 is that Example 1 added citric acid and sodium citrate pH buffers; the buffers resist pH fluctuations caused by raw materials or storage, avoiding the destruction of component activity by acidic environment or the damage to mucous membranes caused by alkaline environment; and enhance product safety and stability; it can be seen that adding citric acid and sodium citrate pH buffers is superior.

[0064] Comparing Examples 1 and 14-17, the inhibition rates of Staphylococcus aureus and Escherichia coli in Example 1 were greater than those in Examples 14-17, and the degradation rates of the active ingredients in Example 1 were less than those in Examples 14-17. In Examples 14-15, the inhibition rates of Staphylococcus aureus and Escherichia coli were greater than those in Examples 16-17, and the degradation rates of the active ingredients in Examples 14-15 were less than those in Examples 16-17.

[0065] The difference between Example 1 and Examples 14-17 is that the mass ratio of sodium citrate to citric acid in Examples 1 and 14-15 is (4-6):1. The pH environment under this ratio is not only unfavorable to the growth of most bacteria, but the stable pH environment can keep pH-sensitive components such as chlorhexidine acetate in a highly active state. It can be seen that the mass ratio of sodium citrate to citric acid in Examples 1 to 1 is better.

[0066] Compared with Examples 1 and 18-19, the antibacterial rates of Staphylococcus aureus and Escherichia coli in Example 1 were greater than those in Examples 18-19, while the degradation rates of the active ingredients in Example 1 were less than those in Examples 18-19.

[0067] The difference between Example 1 and Examples 18-19 is that in Example 1, the mass ratio of sandalwood oil, grape seed oil, sea buckthorn seed oil, polygala root, dodder seed, evodia fruit, Sichuan pepper, chlorhexidine acetate, tert-butylhydroxyanisole, caprylyl glycol, phytic acid-modified cyclodextrin, sodium lauroyl glutamate, propylene glycol, and pH buffer is 250:250:250:70:70:70:30:2:2.5:7.5:10. The mass ratio of sandalwood oil, grape seed oil, sea buckthorn seed oil, polygala root, dodder seed, evodia fruit, Sichuan pepper, chlorhexidine acetate, tert-butylhydroxyanisole, caprylyl glycol, phytic acid-modified cyclodextrin, sodium lauroyl glutamate, propylene glycol, and pH buffer is 250:250:250:70:70:70:30:2:2.5:7.5:10:10:70:3, which is considered optimal.

[0068] Vaginal tightening experiments were conducted on Examples 1 and Comparative Examples 1-2. 150 female volunteers aged 25-35 years were selected and divided into three groups of 50 each. The first group of volunteers applied the antibacterial solution of Example 1 to their vaginas at 8:00 AM and 8:00 PM daily; the second group applied the antibacterial solution of Comparative Example 1 to their vaginas at 8:00 AM and 8:00 PM daily; and the third group applied the antibacterial solution of Comparative Example 2 to their vaginas at 8:00 AM and 8:00 PM daily. All volunteers persisted with the application for 28 days. After application, the vaginas of 45 volunteers in the first group, 42 volunteers in the first group, and 40 volunteers in the second group showed greater vaginal tightness compared to those who did not use the antibacterial solution.

[0069] This specific embodiment is merely an explanation of the present invention and is not intended to limit the present invention. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but as long as they are within the scope of protection claimed by the present invention, they are protected by patent law.

Claims

1. An antibacterial solution with the function of tightening and repairing vaginal mucosal tissue, characterized in that, The components include the following parts by weight: 20-30 parts sandalwood oil; Grape seed oil 20-30; 20-30 parts sea buckthorn seed oil; 5-10 parts of Polygala tenuifolia; 5-10 parts of dodder seed; 5-10 parts of Evodia rutaecarpa; 1-5 parts Sichuan peppercorns; Chlorhexidine acetate 0.1~0.3 parts; Stabilizer 0.5~1.5 parts; The stabilizers include tert-butylhydroxyanisole and caprylyl glycol.

2. The antibacterial solution with tightening and repairing function of vaginal mucosa tissue according to claim 1, characterized in that, Also add 0.5 to 2 parts of phytic acid-modified cyclodextrin.

3. The antibacterial solution with tightening and repairing function of vaginal mucosa tissue according to claim 1, characterized in that, The mass ratio of tert-butylhydroxyanisole to octyl glycol is 1:(1~4).

4. The antibacterial solution with tightening and repairing function of vaginal mucosa tissue according to claim 1, characterized in that, Also add 0.5 to 2 parts of surfactant, wherein the surfactant is sodium lauroyl glutamate.

5. The antibacterial solution with tightening and repairing function of vaginal mucosa tissue according to claim 1, characterized in that, Also add 5 to 10 parts of a humectant, wherein the humectant is one of propylene glycol, 1,3-propanediol, or anhydrous ethanol.

6. The antibacterial solution with tightening and repairing function of vaginal mucosa tissue according to claim 1, characterized in that, Add 0.1 to 0.5 parts of pH buffer, wherein the pH buffer is citric acid and sodium citrate.

7. The antibacterial solution with tightening and repairing function of vaginal mucosa tissue according to claim 6, characterized in that, The mass ratio of sodium citrate to citric acid is (4~6):

1.

8. A production process for an antibacterial solution with vaginal mucosal tissue tightening and repairing function according to any one of claims 1 to 7, characterized in that, Includes the following steps: S1: Add sandalwood oil, grapeseed oil, and sea buckthorn seed oil to a mixing pot and stir to obtain phase A; S2: Grind Polygala tenuifolia, Cuscuta chinensis, Evodia rutaecarpa, and Zanthoxylum bungeanum into powder, sift and mix to obtain a mixed powder; S3: Add the mixed powder to phase A and mix to obtain phase B; S4: Add all components except sandalwood oil, grapeseed oil, sea buckthorn seed oil, polygala root, dodder seed, evodia fruit, and Sichuan pepper to phase B and stir.

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