Seed starting solution, preparation method thereof and method for desensitizing seeds by using seed starting solution

Abstract

The invention provides a seed starting solution, a preparation method thereof and a method for desensitizing seeds by using the seed starting solution, and belongs to the technical field of agricultural biotechnology and food safety. The seed starting solution comprises modified nano-zinc oxide particles dispersed in water, and the modified nano-zinc oxide particles comprise nano-zinc oxide and sodium hexametaphosphate coating the surface of the nano-zinc oxide. The modified nano zinc oxide particles in the seed starting solution can regulate and control the allergic expression of the seeds and reduce the sensitization of the seeds. And the sodium hexametaphosphate belongs to a food additive, is relatively low in cost and almost has no biotoxicity, and the adverse effect of the seed starting solution on the seeds is reduced.

Description

Technical Field

[0001] This invention relates to the fields of agricultural biotechnology and food safety technology, and in particular to a seed activating solution and its preparation method, and a method for desensitizing seeds with the seed activating solution. Background Technology

[0002] Peanuts are an important oilseed crop and a source of protein. However, peanuts contain allergenic proteins such as Ara h1, Ara h2, Ara h3, and Ara h6. These allergenic proteins make peanuts an allergen for some people, leading to severe allergic reactions after consumption. Ara h2 is considered the primary allergenic protein.

[0003] Currently, the main methods for reducing peanut allergenicity are food industry methods such as genetically modified breeding, thermal processing, or enzymatic hydrolysis, while control measures for the field cultivation stage are relatively limited.

[0004] Therefore, it is necessary to find a seed treatment method that can reduce seed allergenicity. Summary of the Invention

[0005] In view of this, in order to at least partially solve the aforementioned technical problems, the present invention provides a seed activating solution and its preparation method, and a method for desensitizing seeds with the seed activating solution.

[0006] According to one aspect of the present invention, a seed-starting solution is provided, comprising modified zinc oxide nanoparticles dispersed in water, the modified zinc oxide nanoparticles comprising zinc oxide nanoparticles and sodium hexametaphosphate coated on the surface of the zinc oxide nanoparticles.

[0007] In some embodiments, the average particle size of the modified nano zinc oxide particles is 40-60 nm.

[0008] In some embodiments, the zeta potential of the modified nano zinc oxide particles is -30 to -20 mV.

[0009] In some embodiments, the modified nano zinc oxide particles in the seed initiation solution have a mass content of 10 to 100 ppm.

[0010] According to another aspect of the present invention, a method for preparing the above-mentioned seed activating solution is provided, comprising: ultrasonically mixing a nano zinc oxide solution with a sodium hexametaphosphate solution to obtain the seed activating solution.

[0011] In some embodiments, the molar concentration of sodium hexametaphosphate in the seed initiation solution is 0.01~0.1mM.

[0012] In some implementations, the ultrasonic mixing power is 120-130W and the time is 5-15 minutes.

[0013] According to another aspect of the present invention, a method for desensitizing seeds using the above-mentioned seed activating solution is provided, comprising: mixing the seed activating solution with seeds to obtain a mixture; and culturing the mixture under light-protected conditions to obtain desensitized seeds.

[0014] In some implementations, the mass ratio of seed initiation solution to seeds is (4~6):1.

[0015] In some implementations, the culture conditions are as follows: cultured on a constant temperature shaker at 15-25°C at a rotation speed of 130-150 rpm for 20-28 hours.

[0016] In some embodiments, before mixing the seed initiation solution with the seeds, the method further includes: disinfecting the seeds with a sodium hypochlorite solution; the seeds include peanuts.

[0017] According to the seed initiation solution of the present invention, sodium hexametaphosphate is used to reverse the electrical potential of the surface of nano-zinc oxide to a negative potential. This makes the modified nano-zinc oxide particles obtained after sodium hexametaphosphate modification more inclined to migrate into the interior of the seed during subsequent co-cultivation with the seed, thereby improving migration efficiency and affecting their contact with the seed tissue. Through the synergistic effect of sodium hexametaphosphate and nano-zinc oxide, it is more beneficial to reduce the expression of allergenic proteins in mature seeds, thereby inhibiting allergic reactions. Attached Figure Description

[0018] The above and other objects, features and advantages of the present invention will become clearer from the following description of embodiments of the invention with reference to the accompanying drawings.

[0019] Figure 1 A transmission electron microscope (TEM) image of modified nano-zinc oxide particles according to a specific embodiment of the present invention is shown.

[0020] Figure 2 The bar charts show the zinc concentrations that migrated into peanuts in Examples 1-3 and Comparative Examples 1-2 of the present invention;

[0021] Figure 3 The following diagrams show a comparison of peanut germination in Examples 4-6 and Comparative Example 1 of the present invention;

[0022] Figure 4 The yield diagrams of peanuts harvested in field planting according to Embodiment 5 and Comparative Examples 1-2 of the present invention are shown.

[0023] Figure 5The bar charts showing the nutrient element content of peanuts harvested from field planting in Embodiment 5 and Comparative Example 1 of the present invention are shown.

[0024] Figure 6 The diagram shows a comparison of gene expression of relevant mRNAs in peanuts harvested from field planting in Example 5 and Comparative Examples 1-2 of the present invention. Detailed Implementation

[0025] Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings. However, it should be understood that these descriptions are exemplary only and are not intended to limit the scope of the invention. In the following detailed description, numerous specific details are set forth to provide a thorough understanding of the embodiments of the invention for ease of explanation. However, it will be apparent that one or more embodiments may be practiced without these specific details. Furthermore, descriptions of well-known structures and techniques are omitted in the following description to avoid unnecessarily obscuring the concept of the invention.

[0026] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the invention. The term "comprising" as used herein indicates the presence of features, steps, or operations, but does not exclude the presence or addition of one or more other features.

[0027] The expression of allergenic proteins is usually influenced by redox signals, reactive oxygen species (ROS) levels, and transcription factor regulation during seed development.

[0028] In the process of realizing the concept of this invention, it was discovered that nano zinc oxide has the potential to regulate ROS levels, alleviate oxidative stress and regulate plant endosperm development, but its effect on the expression of seed allergens is limited.

[0029] To address the aforementioned problems, this invention coats the surface of nano-zinc oxide with sodium hexametaphosphate. Sodium hexametaphosphate reverses the electrical charge on the surface of the nano-zinc oxide to a negative potential, causing the sodium hexametaphosphate-modified nano-zinc oxide to tend to migrate into the seed interior, increasing the migration rate. This allows the regulatory properties of nano-zinc oxide to penetrate deep into the seed interior when blended with the seed, thereby reducing the expression of sensitizing genes in the seed.

[0030] Specifically, according to one embodiment of the present invention, a seed-starting solution is provided, comprising modified zinc oxide nanoparticles dispersed in water, the modified zinc oxide nanoparticles comprising zinc oxide nanoparticles and sodium hexametaphosphate coated on the surface of the zinc oxide nanoparticles.

[0031] According to embodiments of the present invention, coating the surface of nano-zinc oxide with sodium hexametaphosphate facilitates the modification of the nano-zinc oxide, resulting in a negative potential reversal on the surface of the modified nano-zinc oxide particles. This negative potential enhances the efficiency of the nano-zinc oxide entering the seed interior, promoting its contact with seed tissues. The present invention utilizes the nano-zinc oxide in the modified nano-zinc oxide particles to regulate the reactive oxygen species level within the seed, oxidizing and activating MAPK kinase, thereby phosphorylating downstream transcription factors such as WRKY and NAC, thus reducing the expression of allergy genes (e.g., Ara h2) within the seed and decreasing allergic reactions in the population to foods made from the seed. Furthermore, the sodium hexametaphosphate coating layer makes the nano-zinc oxide more stable, largely preventing external environmental influences.

[0032] The nano-zinc oxide used in this invention is relatively inexpensive, promotes seed growth during use, and has low environmental toxicity, making it suitable as the core active ingredient in the seed initiation solution of this invention. Sodium hexametaphosphate is widely used in food additives due to its low biotoxicity and low cost. As a modifier for coating nano-zinc oxide, it offers advantages such as environmental friendliness, low cost, and low toxicity.

[0033] Nano-zinc oxide possesses high surface energy, making it prone to agglomeration in water, forming large particles and thus losing the advantages of its size. Sodium hexametaphosphate, a long-chain polyelectrolyte, can adsorb onto the surface of nano-zinc oxide through its phosphate groups, forming a coating layer. This outward-extending long-chain polyelectrolyte creates physical barriers between multiple nano-zinc oxide particles, preventing them from agglomerating and maintaining the nanoparticle size and properties of the nanomaterial. Furthermore, sodium hexametaphosphate, after ionization, carries a negative charge. Adsorbing onto the surface of nano-zinc oxide imparts a uniform negative charge, causing electrostatic repulsion between particles, resulting in stable dispersion in water and preventing drastic dissolution. This fully leverages the advantages of small size and surface effects at the nanoscale.

[0034] In some embodiments, the average particle size of the modified zinc oxide nanoparticles is 40-60 nm. The average particle size of the modified zinc oxide nanoparticles is slightly larger than that of regular zinc oxide nanoparticles, but much smaller than the micron-sized aggregates formed by unmodified zinc oxide nanoparticles dispersed in water. This demonstrates that the coating modification with sodium hexametaphosphate can effectively inhibit severe and uncontrollable aggregation, adjusting the size of the obtained modified zinc oxide nanoparticles to a lower level. When subsequently mixed with seeds, the smaller size allows for easier migration into the seed interior, affecting contact with seed tissues and consequently influencing ROS levels within the seed, thus inhibiting the expression of sensitizing genes.

[0035] In one specific implementation, such as Figure 1As shown, when 50 nm of nano zinc oxide was added, the average particle size of the modified nano zinc oxide particles was 49.6~57.2 nm, which did not increase significantly. This indicates that the addition of sodium hexametaphosphate inhibited the aggregation phenomenon that nano zinc oxide easily occurs in water, thereby facilitating its migration into the seed interior.

[0036] Optionally, the average particle size of the modified nano zinc oxide particles may be, for example, 40 nm, 45 nm, 49 nm, 50 nm, 51 nm, 52 nm, 54 nm, 55 nm, 56 nm, 57 nm, 58 nm or 60 nm, or a range consisting of any two of the above values.

[0037] It is understood that the average particle size of the modified nano zinc oxide particles can be measured by dynamic light scattering method, and this invention does not impose any particular limitation on this.

[0038] In some embodiments, the zeta potential of the modified zinc oxide nanoparticles is -30 to -20 mV. This setting ensures good dispersion stability, making the surface of the modified zinc oxide nanoparticles exhibit a negative potential, which encourages them to migrate into the interior of the seed, further improving migration efficiency.

[0039] Optionally, the zeta potential of the modified nano zinc oxide particles may be, for example, -30mV, -28.79mV, -25mV or -20mV, or a range consisting of any two of the above values.

[0040] In some embodiments, the modified nano-zinc oxide particles in the seed initiation solution have a mass content of 10-100 ppm. This setting results in a larger particle spacing, and the negative potential of the particle surface ensures more uniform particle dispersion in the seed initiation solution, reducing the risk of aggregation and sedimentation. This also facilitates migration into the seed interior during subsequent seed desensitization processes, thereby reducing seed sensitization.

[0041] Optionally, the mass content of the modified nano zinc oxide particles is 10 ppm, 30 ppm, 50 ppm, 70 ppm or 100 ppm, or a range consisting of any two of the above values.

[0042] According to another aspect of the present invention, a method for preparing the above-mentioned seed activating solution is provided, comprising: ultrasonically mixing a nano zinc oxide solution with a sodium hexametaphosphate solution to obtain the seed activating solution.

[0043] According to embodiments of the present invention, ultrasonic mixing facilitates efficient and uniform coating of sodium hexametaphosphate on the surface of nano-zinc oxide, contributing to the formation of a highly stable, small-particle-size dispersed seed initiation solution and preventing particle aggregation. Furthermore, the uniform coating of sodium hexametaphosphate uniformly modifies the surface of the nano-zinc oxide to electronegativity, thereby enhancing its migration efficiency into the seed interior. This, in turn, reduces the expression level of sensitizing genes within the seed after subsequent desensitization treatment.

[0044] In some embodiments, the molar concentration of sodium hexametaphosphate in the seed initiation solution is 0.01~0.1 mM. This setting utilizes the electrostatic repulsion provided by sodium hexametaphosphate to effectively disperse the modified zinc oxide nanoparticles. This concentration ensures effective dispersion while avoiding the potential increase in ionic strength caused by excessive sodium hexametaphosphate, which could compress the electric double layer around the modified zinc oxide nanoparticles, weakening the electrostatic repulsion and leading to undesirable aggregation. Furthermore, during subsequent mixing with seeds, an excessively high molar concentration would result in overly high electronegativity of the modified zinc oxide nanoparticles, causing them to repel the seeds and thus reducing their migration efficiency within the seed tissue.

[0045] Optionally, the molar concentration of sodium hexametaphosphate may be, for example, 0.01 mM, 0.02 mM, 0.03 mM, 0.04 mM, 0.05 mM, 0.06 mM, 0.07 mM, 0.08 mM, 0.09 mM or 0.1 mM, or a range consisting of any two of the above values.

[0046] In some embodiments, the ultrasonic mixing power is 120-130W, and the time is 5-15 minutes. This setting helps to improve the uniform dispersion of modified zinc oxide nanoparticles in the seed initiation solution, avoids the violent dissolution and agglomeration of multiple modified zinc oxide nanoparticles, and further improves the migration efficiency of subsequent migration into the seed interior.

[0047] Optionally, the power of the ultrasonic mixing can be 120W, 125W, or 130W, or a range consisting of any two of the above values.

[0048] Optionally, the ultrasound duration can be 5 min, 10 min, or 15 min, or a range consisting of any two of the above values.

[0049] It can be understood that the nano zinc oxide solution is an aqueous solution of nano zinc oxide. The sodium hexametaphosphate solution is an aqueous solution of sodium hexametaphosphate.

[0050] According to another aspect of the present invention, a method for desensitizing seeds using the above-mentioned seed activating solution is provided, comprising: mixing the seed activating solution with seeds to obtain a mixture; and culturing the mixture under light-protected conditions to obtain desensitized seeds.

[0051] According to embodiments of the present invention, the surface modification of nano-zinc oxide with sodium hexametaphosphate imparts a negative potential, which facilitates the migration of modified nano-zinc oxide particles into the seed interior. Under light-protected conditions, the modified nano-zinc oxide particles that have migrated into the seed interior can generate reactive oxygen species, inhibiting the expression of allergenic proteins in the seed and thus reducing allergic reactions after consumption by the organism population. The desensitization method of the present invention can reduce the expression of allergenic proteins at the seed initiation stage, reducing the allergenicity of seed products from the source, and has broad application prospects in agriculture.

[0052] In some embodiments, the mass ratio of seed activating solution to seeds is (4~6):1. This setting allows the seed activating solution to completely and uniformly coat each seed, creating a sufficient treatment microenvironment. This ensures that the modified nano-zinc oxide particles can fully migrate into the interior of each seed, resulting in a relatively uniform desensitization effect on multiple seeds. If the amount of seed activating solution is too low, it can only wet the seed surface and is difficult to penetrate into the fine folds and pores of the seed coat, making it difficult to penetrate into the seed interior. If the amount of seed activating solution is too high, it results in waste of the seed activating solution, increases costs, and may require additional drying steps, making the process more complex.

[0053] Optionally, the mass ratio between the seed initiation solution and the seeds can be, for example, 4:1, 5:1, or 6:1, or a range formed between any two of the above ratios.

[0054] In some embodiments, the cultivation conditions are as follows: cultivation on a constant-temperature shaker at 15-25°C and a rotation speed of 130-150 rpm for 20-28 hours. This setup helps create a uniform contact environment for the seeds through continuous shaking at the aforementioned suitable temperature, and completes the desensitization treatment of the seeds within the aforementioned time window, while maximizing the activation of the seeds' growth potential. The aforementioned temperature can maintain and activate the metabolic enzyme activity of the seeds. The aforementioned shaking speed can continuously supply nutrients, maintain aerobic respiration of the seeds, and prevent the modified zinc oxide nanoparticles from settling due to gravity, ensuring their interaction with the seeds. The aforementioned time provides a sufficient time window for the modified zinc oxide nanoparticles to migrate into the seed interior.

[0055] Alternatively, the culture temperature may be, for example, 15°C, 20°C, or 25°C, or a range consisting of any two of the above values.

[0056] Optionally, the rotation speed of the thermostatic shaker can be, for example, 130 rpm, 140 rpm, or 150 rpm, or a range consisting of any two of the above values.

[0057] Alternatively, the incubation time may be, for example, 20h, 24h, or 28h, or a range consisting of any two of the above values.

[0058] In some embodiments, before mixing the seed activating solution with the seeds, the method further includes disinfecting the seeds with a sodium hypochlorite solution. This arrangement removes pathogens that may arise during seed growth using the sodium hypochlorite solution.

[0059] Optionally, the sodium hypochlorite solution may contain 5% sodium hypochlorite by mass.

[0060] In some embodiments, the seeds include peanuts. It is understood that the main allergenic protein in peanuts is Ara h2. By utilizing sodium hexametaphosphate to coat nano-zinc oxide for the aforementioned desensitization treatment of peanut seeds before sowing, this invention can significantly inhibit the expression level of Ara h2 in mature peanuts, thereby reducing the allergenicity of mature peanuts and expanding the application range of peanut products.

[0061] It should be noted that the method of this invention avoids further processing at the food industry level, such as genetic modification, thermal processing, or enzymatic hydrolysis. The desensitization treatment is only performed during the field cultivation stage, simplifying the processing steps and significantly reducing the allergenicity of the raw peanuts, thus having broad application scenarios. The raw materials used in this invention are relatively easy to obtain and have low cost, which facilitates its widespread promotion in agricultural agronomy.

[0062] The present invention will be further illustrated below through embodiments, related test experiments, and their results. In the following detailed description, numerous specific details are set forth for ease of explanation to provide a comprehensive understanding of the embodiments of the present invention. However, it will be apparent that one or more embodiments may be practiced without these specific details. Moreover, the details in the following embodiments can be arbitrarily combined to form other feasible embodiments without conflict.

[0063] It should be noted that the specific embodiments described below are merely illustrative examples, and the scope of protection of this invention is not limited thereto. The chemicals and raw materials used in the following embodiments are all commercially available or prepared using recognized processing methods.

[0064] Example 1:

[0065] Preparation of seed starter solution:

[0066] A 500 mg / L aqueous solution of nano-zinc oxide was prepared by dissolving 50 nm average particle size in zinc oxide nanoparticles, and an aqueous solution of sodium hexametaphosphate was also prepared.

[0067] The above-mentioned nano zinc oxide solution and sodium hexametaphosphate solution were ultrasonically mixed at a power of 125W for 10 minutes to perform ultrasonic modification, thereby obtaining modified nano zinc oxide particles dispersed in water, which in turn yielded a seed starter solution. The molar concentration of sodium hexametaphosphate in the seed starter solution was adjusted to 0.01mM.

[0068] The desensitization process for peanut seeds:

[0069] Peanut seeds were disinfected using a 5% sodium hypochlorite solution. Then, a seed initiation solution was prepared and mixed with the peanut seeds at a mass ratio of 1:5. The mixture was then incubated on a constant-temperature shaker at 25°C and 140 rpm in the dark for 24 hours.

[0070] After cultivation, the peanut seeds were rinsed three times with ultrapure water to remove any remaining modified nano zinc oxide particles from the surface.

[0071] Example 2:

[0072] The preparation process of Example 2 is largely the same as that of Example 1, except that the molar concentration of sodium hexametaphosphate in the seed initiation solution in this example is 0.1 mM.

[0073] Transmission electron microscopy (TEM) was performed on the modified zinc oxide nanoparticles of Example 2, as shown in the results. Figure 1 As shown, the average particle size of the modified nano zinc oxide particles is 49.6~57.2 nm, indicating that the coating with sodium hexametaphosphate did not significantly increase the particle size of the nano zinc oxide, allowing it to penetrate into the interior of the seed through pores and other channels.

[0074] Example 3:

[0075] The preparation process of Example 3 is largely the same as that of Example 1, except that the molar concentration of sodium hexametaphosphate in the seed initiation solution in this example is 1 mM.

[0076] Comparative Example 1:

[0077] Comparative Example 1 is a blank control experiment, in which peanut seeds were disinfected using only a 5% sodium hypochlorite solution.

[0078] Comparative Example 2:

[0079] The preparation process of Comparative Example 2 is largely the same as that of Example 1, except that sodium hexametaphosphate was not added in Comparative Example 2. Instead, the solution formed by the nano-zinc oxide particles was directly mixed with peanut seeds. The peanut seeds were then treated with a nano-zinc oxide solution of the same concentration as in Example 1.

[0080] Peanut seeds from Examples 1-3 and Comparative Examples 1-2 were microwave digested using 6 mL of nitric acid. Each experiment was repeated three times. The zinc content of the digested solutions was quantified using inductively coupled plasma mass spectrometry (ICP-MS). Figure 2 The bar charts show the zinc concentrations migrated into peanuts in Examples 1-3 and Comparative Examples 1-2 of the present invention. Figure 2 As shown, the zinc content in peanut seeds is relatively high when the sodium hexametaphosphate concentration is 0.1 mM, indicating that the modified zinc oxide nanoparticles can maintain high migration efficiency in peanut seeds at a sodium hexametaphosphate concentration of 0.1 mM. The zeta potential of the modified zinc oxide nanoparticles prepared in Example 2 was measured using dynamic light scattering, and it was -28.79 mV, while the zeta potential of the unmodified zinc oxide nanoparticles prepared in Comparative Example 2 was 25.34 mV. This indicates that the addition of sodium hexametaphosphate can reverse the electrical charge on the surface of the zinc oxide nanoparticles to a negative potential. This change in potential allows the modified zinc oxide nanoparticles to penetrate the seed interior, affecting their contact with the seed tissues and thus regulating the ROS (reactive oxygen species) level within the seed, thereby influencing the sensitization expression in peanuts.

[0081] Example 4:

[0082] The preparation process of Example 4 is largely the same as that of Example 2, except that the concentration of modified nano zinc oxide particles in the seed starting solution is adjusted to 10 ppm, and then the peanut seeds are desensitized in the same way as in Example 2.

[0083] Example 5:

[0084] The process in Example 5 is largely the same as that in Example 4, except that the concentration of modified nano zinc oxide particles in the seed starting solution is adjusted to 50 ppm.

[0085] Example 6:

[0086] The process in Example 6 is largely the same as that in Example 4, except that the concentration of the modified nano zinc oxide particles in the seed starting solution is adjusted to 100 ppm.

[0087] To investigate the effect of modified nano-zinc oxide particles on the germination rate of peanut seeds, the germination rates of peanuts from Examples 4-6 and Comparative Example 1 were tested. Moistened filter paper was placed in 25cm diameter petri dishes, and 15 uniformly sized peanut seeds from Examples 4-6 and Comparative Example 1 were placed on the filter paper. The petri dishes were incubated at 25℃, 75% humidity, and in the dark for 7 days. After incubation, seeds with a sprout tip of 5cm were considered to have successfully germinated. Figure 3 The diagram shows a comparison of peanut germination in Examples 4-6 and Comparative Example 1 of the present invention. Figure 3 As shown, it can be seen that the germination of Examples 1 to 3 is improved compared to Comparative Example 1, with Example 5 having a relatively higher germination rate.

[0088] Peanut seeds obtained in Example 2 and Comparative Examples 1-2 were used in field experiments. It should be noted that the field experiments were conducted in Beifang Town, Huairou District, Beijing, and the planting period for all experiments was from May to October 2025, a total of 6 months, with a total cultivated area of ​​4.9 mu.

[0089] Apart from the treatment of peanut seeds as described in Example 5 and Comparative Examples 1-2, all other agronomic practices followed local farmers' farming practices without additional treatments. After sampling, the yield of peanuts in each treatment group was measured by thousand-kernel weight. Figure 4 The diagram shows the yield of peanuts harvested in field planting according to Embodiment 5 and Comparative Examples 1-2 of the present invention. Figure 4 As shown, the yield of peanut seeds treated with 50 ppm of modified nano zinc oxide particles was increased compared with both Comparative Examples 1 and 2, with an increase of approximately 8.9% compared with untreated peanuts.

[0090] The nutrient elements in peanuts obtained from field cultivation in Example 5 and Comparative Example 1 were measured. The measurement process is as follows: one peanut seed was microwave-digested with 6 mL of concentrated nitric acid, and the experiment was repeated three times in parallel. The nutrient elements in the digested solution were quantified using inductively coupled plasma mass spectrometry (ICP-MS). Figure 5 The bar charts showing the nutrient element content of peanuts harvested from field planting in Embodiment 5 and Comparative Example 1 of the present invention are illustrated. Figure 5 As shown, after soaking peanut seeds with the seed activating solution of the present invention, they can maintain growth relatively better under subsequent photosynthesis and have a stronger ability to absorb trace elements in the soil.

[0091] The gene expression of relevant mRNAs from peanuts harvested in field planting in Examples 5 and 1-2 was tested. The expression process of the allergic protein was as follows: 1 mL of RNA extraction buffer (Trizol) was added to each harvested peanut and lysed thoroughly. The mixture was transferred to a centrifuge tube and mixed by pipetting, then incubated at room temperature for 3 min. The mixture was then centrifuged at 12000 rpm for 5 min. The supernatant was collected, and 200 μL of chloroform / mL Trizol was added. The mixture was inverted and incubated at room temperature for 10 min. Next, the mixture was centrifuged at 12000 rpm at 4°C for 15 min. The supernatant was carefully aspirated and transferred to another centrifuge tube. An equal volume of isopropanol was added, and the mixture was incubated at 4°C for 10 min. The mixture was then centrifuged at 12000 rpm at 4°C for 10 min. The supernatant was discarded, and 500 μL of 75% ethanol was added for washing once. After opening the lid and allowing the solution to air dry at room temperature, add 20 μL of RNase-free ultrapure water to dissolve the RNA precipitate. Vortex at room temperature for 5 min to ensure complete RNA dissolution. Measure the expression of the peanut allergy protein Ara h2 using quantitative real-time PCR. Figure 6 The diagram shows a comparison of gene expression of relevant mRNAs in peanuts harvested from field planting, based on Example 5 and Comparative Examples 1-2 of the present invention. Figure 6 As shown, Example 5 of the present invention exhibits a significant decrease in the expression of the peanut allergy protein Ara h2 compared to Comparative Examples 1 and 2, with a decrease of over 99.5%. It can also be seen that Comparative Example 2, without sodium hexametaphosphate treatment of the nano-zinc oxide, results in a positively charged surface on the nano-zinc oxide, affecting its contact with cell tissues and consequently influencing ROS levels and allergy expression, leading to more pronounced allergic expression in peanuts. In contrast, the present invention, through the use of sodium hexametaphosphate and modification of the nano-zinc oxide, allows the modified nano-zinc oxide particles to migrate into the peanut seed interior, affecting intracellular ROS levels and reducing peanut allergenicity at its source, demonstrating broad application prospects in agriculture.

[0092] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above descriptions are merely specific embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A seed priming solution, comprising modified nano-zinc oxide particles dispersed in water, wherein the modified nano-zinc oxide particles comprise nano-zinc oxide and sodium hexametaphosphate coated on the surface of the nano-zinc oxide.

2. The seed priming solution of claim 1, wherein, The average particle size of the modified nano-zinc oxide particles is 40-60 nm.

3. The seed priming solution of claim 1, wherein, The zeta potential of the modified nano-zinc oxide particles is -30--20 mV. The mass content of the modified nano-zinc oxide particles in the seed priming solution is 10-100 ppm. 4.A method for preparing the seed priming solution according to any one of claims 1-3, comprising: mixing a nano-zinc oxide solution with a sodium hexametaphosphate solution by ultrasonic mixing to obtain the seed priming solution.

5. The method of formulating according to claim 4, wherein, The molar concentration of sodium hexametaphosphate in the seed priming solution is 0.01-0.1 mM.

6. The method of formulating according to claim 4, wherein, The power of ultrasonic mixing is 120-130 W, and the time is 5-15 min. 7.A method for desensitizing seeds using the seed priming solution according to any one of claims 1-3, comprising: mixing the seed priming solution with seeds to obtain a mixture; culturing the mixture in the dark to obtain desensitized seeds.

8. The method of claim 7, wherein, The mass ratio between the seed priming solution and the seeds is (4-6) :

1.

9. The method of claim 7, wherein, The culturing condition is culturing at 130-150 rpm for 20-28 h in a constant-temperature shaker at 15-25 ℃.

10. The method of claim 7, wherein, Before mixing the seed priming solution with the seeds, the method further comprises: sterilizing the seeds with a sodium hypochlorite solution; the seeds comprise peanuts.

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