Preparation method and use method of microbial agent for improving water body and improvement equipment

Abstract

The invention provides a preparation method and a use method of a microbial agent for improving a water body and improvement equipment. The preparation method comprises the following steps: step 1, selecting and culturing a multifunctional strain: a, selecting a microbial strain with functions of degrading organic matters, removing nitrogen and removing phosphorus from a strain library; b, activating the selected strains by adopting a culture medium containing a carbon source, a nitrogen source and inorganic salt; and c, carrying out amplification culture in a constant-temperature shaking table to ensure the purity and activity of the strain. By selecting and culturing the microbial strains with the functions of degrading organic matters, removing nitrogen and removing phosphorus, various pollutants in a water body can be efficiently treated, the water quality is improved, the anode of the MFC is made of the carbon nanotube composite material, the conductivity and biocompatibility of the electrode are improved, attachment and growth of the microbial strains are facilitated, and the service life of the MFC is prolonged. Therefore, the processing efficiency and the electric energy output of the MFC are enhanced.

Description

Technical Field

[0001] This invention relates to the field of wastewater or sludge treatment, and more specifically, to a method for preparing, using, and using microbial agents for improving water quality. Background Technology

[0002] Traditional water treatment methods are often costly, inefficient, and may cause secondary pollution. Therefore, developing an efficient, environmentally friendly, and cost-effective water pollution treatment method has become an urgent task. For example, the Chinese invention patent CN115093078A discloses "A purification and remediation agent for saline-alkali polluted water and its preparation method and application". Its specification states that how to effectively treat high saline water pollution in arid areas is one of the environmental problems that urgently need to be solved in water-scarce areas of the world. The above patent can prove the defects of the existing technology.

[0003] Therefore, we have made improvements to this, proposing methods for preparing and using microbial agents to improve water quality, as well as methods for improving equipment. Summary of the Invention

[0004] The purpose of this invention is to address the fact that existing traditional water treatment methods are often costly, inefficient, and may cause secondary pollution.

[0005] To achieve the above-mentioned objectives, the present invention provides the following methods for preparing, using, and improving equipment for microbial agents to improve water quality, thereby addressing the aforementioned problems.

[0006] This application specifically includes the following steps: Step 1: Selecting and culturing multifunctional strains: a. Select microbial strains (denitrifying bacteria, polyphosphate-accumulating bacteria, and strains capable of decomposing specific organic matter) from the strain bank. b. Activate the selected bacterial strains using a culture medium containing carbon sources, nitrogen sources, and inorganic salts; c. Expand the culture in a constant temperature shaker to ensure the purity and activity of the strain; Step 2: Fabrication of Microbial Fuel Cell (MFC) Electrodes: a. Using carbon nanotube composite materials to fabricate the anode of a microbial fuel cell. Carbon nanotube composite materials have high conductivity and good biocompatibility, which can be used to support microbial attachment and biofilm formation. b. A cathode coated with a platinum catalyst is used to promote the oxygen reduction reaction; c. Pre-treat the electrodes, including cleaning, drying, and sterilization, to ensure sterile conditions; Step 3: Integrating microbial fuel cells and microbial agents: a. The cultured multifunctional microbial strains are evenly coated on the anode of the microbial fuel cell to ensure that the strains adhere firmly; b. Add the water sample to be treated into the microbial fuel cell device to ensure good contact between the anode, cathode, and electrolyte; c. Connect an external circuit to monitor and record the electrical output of the microbial fuel cell; Step 4: Fermentation and Optimization a. The device integrating the microbial fuel cell was placed in a constant temperature and humidity environment for 48 hours of fermentation; b. Optimize the degradation efficiency and power generation of the strain by adjusting fermentation conditions, including temperature, pH and dissolved oxygen; c. Monitor the power output of MFC in real time. When the power output reaches a stable level, fermentation is considered complete. Step 5: Preparation of microbial inoculants: a. After fermentation is complete, disconnect the external circuit of the MFC and remove the anode; b. Using aseptic techniques, scrape and rinse the attached microorganisms from the anode to form a uniform microbial suspension; c. Add a protectant (glycerol) and a stabilizer (trehalose) to the microbial suspension to ensure the survival rate and stability of the strain; d. Conduct quality testing on the microbial agent, including indicators of strain activity, purity, and quantity.

[0007] As a preferred technical solution of this application, in step three, the cultured multifunctional microbial strain is uniformly coated onto the anode of the microbial fuel cell. The specific coating process includes the following steps: Preparation of bacterial suspension: Take the cultured multifunctional microbial strain out of the culture medium, wash it with buffer and physiological saline to remove culture medium residue and impurities, and resuspend the strain in an appropriate amount of buffer and physiological saline to prepare a bacterial suspension. Anode surface treatment: Before coating the bacterial strain, the anode surface of the MFC needs to be subjected to plasma treatment, chemical etching and physical roughening treatment to increase the roughness of the anode surface, so as to increase the adhesion of the bacterial strain and provide more adhesion points for the bacterial strain. Coating the bacterial suspension: The prepared bacterial suspension is evenly coated on the anode surface of the MFC. The bacterial suspension is added drop by drop to the anode using a pipette to ensure that the bacterial suspension covers the entire anode surface and is evenly distributed.

[0008] As a preferred technical solution of this application, in step three, the cultured multifunctional microbial strain is uniformly coated onto the anode of the microbial fuel cell. The specific coating process further includes the following steps: Drying and Fixation: After coating the bacterial suspension, place the anode in a sterile environment and allow the bacterial suspension to dry naturally. During the drying process, the bacterial strain will gradually adhere to the anode surface. Use electric current stimulation and chemical cross-linking agent to fix the bacterial strain to ensure that the bacterial strain adheres firmly. Inspection and verification: After coating, the adhesion of the bacterial strain on the anode surface is inspected under a microscope, and the performance of the MFC is measured to verify whether the bacterial strain has successfully adhered.

[0009] The device for improving water quality with microbial agents includes a hollow rod and a mounting plate fixedly connected to it. The hollow rod is equipped with a circulation device, a drive assembly, and a feeding assembly.

[0010] As a preferred technical solution of this application, the cyclic action device includes a first hinge block fixedly connected to the hollow part of the hollow rod, a second hinge block slidably connected to the hollow rod via a sliding block, two first hinge rods hinged to the first hinge block, two second hinge rods hinged to the second hinge block, the ends of the first hinge rods and the second hinge rods being hinged together, the cyclic action device also includes a base fixedly connected to the hollow rod, a hollow telescopic rod fixedly connected to the hollow rod, the other end of the hollow telescopic rod being fixedly connected to the second hinge block, a connecting spring fixedly connected to the side of the second hinge block away from the hollow telescopic rod, and the other end of the connecting spring being fixedly connected to the hollow rod.

[0011] As a preferred technical solution of this application, the circulation device further includes a wire harness wheel fixedly connected to the outer wall of the hollow rod, two drive motors fixedly connected to the base, and a rigid rack fixedly connected to the output end of the drive motors via a coupling. The circulation device also includes an arc-shaped plate fixedly connected to the rigid rack, a first filter plate fixedly connected to one side of the arc-shaped plate, and a second filter plate fixedly connected to the other side of the arc-shaped plate.

[0012] As a preferred technical solution of this application, the driving component includes a winding wheel rotatably connected to the base, a connecting rope on the winding wheel, a support platform fixedly connected to the second hinge block, a fixing block fixedly connected to the bottom outer wall of the hollow rod, and the end of the rigid rack away from the drive motor rotatably connected to the fixing block through a bearing. A winding rope is provided on the winding wheel.

[0013] As a preferred technical solution of this application, the feeding assembly includes a main shaft rotatably connected to the hinge of the first hinge rod and the second hinge rod. A fixing plate is fixedly connected to the bottom of the main shaft. A storage cylinder is fixedly connected to the top outer wall of the first hinge rod. A auger is rotatably connected in the storage cylinder. A transmission gear is fixedly sleeved on the main shaft. The auger is fixedly connected to the main shaft.

[0014] As a preferred technical solution of this application, the feeding component further includes a rectangular groove formed on the fixed plate, a first feeding nozzle is fixedly connected to the rectangular groove, and a second feeding nozzle is fixedly connected to the bottom of the second hinge block.

[0015] The method of using equipment for improving water quality with microbial agents includes: Ⅰ. In its initial state, the device is installed on both sides of the hull via mounting plates and mounting components, and is available for three different usage scenarios; Scenario 1: A microbial agent for purification is applied to a small body of water. After installation, a motor inside the base drives a winding reel to rotate. The reel transmits force through a connecting rope. This rope pulls the second hinge block to slide on the hollow rod. As the second hinge block moves, it stretches the connecting spring. At this point, the first and second hinge rods move around the hinge point, forming a... Figure 3 and Figure 4 During the movement, it can be observed that the transmission gear gradually meshes with the rigid rack and rotates. As the main shaft at the bottom of the hinge point moves the fixed plate, it pushes aside the weeds on the water surface and rotates itself. During the rotation of the main shaft, the winch outputs the material in the storage cylinder through the first feed nozzle. In addition, the fixed plate can more effectively spread and stir the material during the rotation. At the same time, the hollow telescopic rod is compressed due to the movement of the second hinge block, so the agent in the hollow part of the hollow telescopic rod is output into the water through the second feed nozzle. Ⅱ. Scenario 2: In a large water area, as the ship moves, the water flow drives the fixed plate to rotate. At this time, the fixed plate can drive the auger inside the storage cylinder to rotate through the main shaft. No external driving energy is needed to spread the material in the storage cylinder. At the same time, the upper arc plate and the first and second filter plates on both sides block flying objects in the water area to prevent damage to the equipment body. Furthermore, the extended first and second hinge rods can enhance the stability of the arc plate and improve its protective capability. Ⅲ. Scenario 3: High-altitude waters that require climbing. Place the capsule-shaped microbial agent on the support platform, hold the fixing plate and pass it through the rectangular slot. At this time, the rectangular slot and the fixing plate act as handles. After the second hinge block is moved to the position where it coincides with the arc plate by the rotation of the reel, place the microbial agent on the support platform. At this time, the arc plate acts as a wind deflector to prevent the wind from the sides from interfering with the launch. The connecting spring is stretched due to the movement of the second hinge block. After aiming at the water area to be released, the connecting rope can be cut. At this time, the second hinge block is ejected to the top, and the microbial agent is launched into the designated water area. IV. When the hull is stationary, the drive motor rotates through the output end to drive the rigid rack to rotate. The rigid rack drives the arc plate to follow the movement, and the two rigid racks come together to form a grab to salvage the floating object. V. When the ship is in motion, the water flows through the first filter plate with a larger aperture and is then collected by the second filter plate with a smaller aperture. This prevents larger impurities from entering and causing overload, which could damage the equipment.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: In the scheme of this application: 1. By selecting and cultivating microbial strains with functions of degrading organic matter, denitrification, and phosphorus removal, multiple pollutants in water can be efficiently treated, improving water quality. The use of carbon nanotube composite materials to make the anode of the MFC improves the conductivity and biocompatibility of the electrode, which is conducive to the attachment and growth of microbial strains, thereby enhancing the treatment efficiency and power output of the MFC. Compared with traditional water treatment methods, the microbial fuel cell technology adopted in this invention does not require the addition of chemical agents, avoids secondary pollution, and can generate electricity, achieving the dual goals of wastewater treatment and energy recovery, which meets the requirements of environmental protection and sustainable development. The preparation method of this invention is relatively simple, easy to operate and control. At the same time, since the MFC technology can use organic matter in wastewater as fuel, the operating cost is reduced. 2. In order to solve the problem that the device cannot be well protected during the movement of the ship in the prior art, this application achieves the filtering and collection of specific impurities during the movement by setting two filter plates, while also protecting the device body. 3. By setting up a cyclic action device, the functionality of the device body was changed, and the problem of not being able to project the agent into higher water areas in the prior art was solved by transforming the device body into a transmitter; 4. By setting up the drive components, the switching between inherent power and environmental power is realized, making full use of the power of the ship's movement and solving the problem of insufficient utilization of excess energy in the existing technology; 5. By setting the drive components, the functionality of the device can be switched, turning the device into a catapult, which allows the microbial agent to be launched into the water at a higher level by catapult. Attached Figure Description

[0017] Figure 1 The flowchart of the preparation method of the microbial agent for improving water quality provided in this application Figure 2 A front view of the water-improving microbial agent equipment provided in this application; Figure 3 A schematic diagram of the second state of the water-improving microbial agent provided in this application for improving water quality; Figure 4 A schematic diagram of the internal structure of the water-improving microbial agent provided in this application; Figure 5 A cross-sectional view of the water-improving microbial agent equipment provided in this application; Figure 6 A schematic diagram of the third state of the water-improving microbial agent equipment provided in this application; Figure 7 A schematic diagram of the hollow rod structure of the water-improving microbial agent for water body improvement equipment provided in this application; Figure 8 A schematic diagram of the base structure of the water-improving microbial agent for water body improvement equipment provided in this application; Figure 9 A schematic diagram of the main shaft structure of the water-improving microbial agent for water body improvement provided in this application; Figure 10 A schematic diagram of the internal structure of the storage tank of the water-improving microbial agent for water body improvement equipment provided in this application; Figure 11 A schematic diagram of the support platform structure of the water-improving microbial agent provided in this application.

[0018] The image shows: 1. Hollow rod; 2. Mounting plate; 3. Circulation device; 301. First hinge block; 302. Second hinge block; 303. First hinge rod; 304. Second hinge rod; 305. Base; 306. Hollow telescopic rod; 307. Connecting spring; 308. Cable pulley; 309. Drive motor; 310. Rigid rack; 311. Arc plate; 312. First filter plate; 313. Second filter plate; 4. Drive assembly; 401. Reel; 402. Connecting rope; 403. Support platform; 404. Fixing block; 405. Reel; 5. Feeding assembly; 501. Main shaft; 502. Fixing plate; 503. Storage cylinder; 504. Screw; 505. Transmission gear; 506. Rectangular groove; 507. First feeding nozzle; 508. Second feeding nozzle. Detailed Implementation

[0019] To enable those skilled in the art to better understand the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

[0020] As described in the background section, traditional water treatment methods are often costly, inefficient, and may cause secondary pollution.

[0021] To address this technical problem, the present invention provides a method for preparing, using, and improving equipment for microbial agents that can improve water quality, which is applied to the efficient preparation and rational utilization of microbial agents.

[0022] Specifically, such as Figure 1 As shown, the preparation method of the microbial agent for improving water quality includes the following steps: Step 1: Selecting and culturing multifunctional strains: a. Select microbial strains (denitrifying bacteria, polyphosphate-accumulating bacteria, and strains capable of decomposing specific organic matter) from the strain bank. b. Activate the selected bacterial strains using a culture medium containing carbon sources, nitrogen sources, and inorganic salts; c. Expand the culture in a constant temperature shaker to ensure the purity and activity of the strain; Step 2: Fabrication of Microbial Fuel Cell (MFC) Electrodes: a. Using carbon nanotube composite materials to fabricate the anode of a microbial fuel cell. Carbon nanotube composite materials have high conductivity and good biocompatibility, which can be used to support microbial attachment and biofilm formation. b. A cathode coated with a platinum catalyst is used to promote the oxygen reduction reaction; c. Pre-treat the electrodes, including cleaning, drying, and sterilization, to ensure sterile conditions; Step 3: Integration of fuel cells and microbial agents: a. The cultured multifunctional microbial strains are evenly coated on the anode of the microbial fuel cell to ensure that the strains adhere firmly; b. Add the water sample to be treated into the microbial fuel cell device to ensure good contact between the anode, cathode, and electrolyte; c. Connect an external circuit to monitor and record the electrical output of the microbial fuel cell; Step 4: Fermentation and Optimization a. The device integrating the microbial fuel cell was placed in a constant temperature and humidity environment for 48 hours of fermentation; b. Optimize the degradation efficiency and power generation of the strain by adjusting fermentation conditions, including temperature, pH and dissolved oxygen; c. Monitor the power output of MFC in real time. When the power output reaches a stable level, fermentation is considered complete. Step 5: Preparation of microbial inoculants: a. After fermentation is complete, disconnect the external circuit of the MFC and remove the anode; b. Using aseptic techniques, scrape and rinse the attached microorganisms from the anode to form a uniform microbial suspension; c. Add a protectant (glycerol) and a stabilizer (trehalose) to the microbial suspension to ensure the survival rate and stability of the strain; d. Conduct quality testing on the microbial agent, including indicators of strain activity, purity, and quantity.

[0023] In step three, the cultured multifunctional microbial strains are uniformly coated onto the anode of the microbial fuel cell. The specific coating process includes the following steps: Preparation of bacterial suspension: Take the cultured multifunctional microbial strain out of the culture medium, wash it with buffer and physiological saline to remove culture medium residue and impurities, and resuspend the strain in an appropriate amount of buffer and physiological saline to prepare a bacterial suspension. Anode surface treatment: Before coating the bacterial strain, the anode surface of the MFC needs to be subjected to plasma treatment, chemical etching and physical roughening treatment to increase the roughness of the anode surface, so as to increase the adhesion of the bacterial strain and provide more adhesion points for the bacterial strain. Coating the bacterial suspension: The prepared bacterial suspension is evenly coated on the anode surface of the MFC. The bacterial suspension is added drop by drop to the anode using a pipette to ensure that the bacterial suspension covers the entire anode surface and is evenly distributed.

[0024] In step three, the cultured multifunctional microbial strains are uniformly coated onto the anode of the microbial fuel cell. The coating process further includes the following steps: Drying and Fixation: After coating the bacterial suspension, place the anode in a sterile environment and allow the bacterial suspension to dry naturally. During the drying process, the bacterial strain will gradually adhere to the anode surface. Use electric current stimulation and chemical cross-linking agent to fix the bacterial strain to ensure that the bacterial strain adheres firmly. Inspection and verification: After coating, the adhesion of the bacterial strain on the anode surface is inspected under a microscope, and the performance of the MFC is measured to verify whether the bacterial strain has successfully adhered.

[0025] It should be noted that, unless otherwise specified, the embodiments and features and technical solutions in the present invention can be combined with each other.

[0026] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0027] Example 1, please refer to Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 The device for improving water quality with microbial agents includes a hollow rod 1 and a mounting plate 2 fixedly connected above it. A circulation device 3 is installed on the hollow rod 1, a drive assembly 4 is installed on the hollow rod 1, and a feeding assembly 5 is installed on the hollow rod 1.

[0028] Please refer to Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 The device for improving water quality with microbial agents includes a circulation device 3 comprising a first hinge block 301 fixedly connected to the hollow part of a hollow rod 1, a second hinge block 302 slidably connected to the hollow rod 1 via a sliding block, two first hinge rods 303 hinged to the first hinge block 301, and two second hinge rods 304 hinged to the second hinge block 302, with the ends of the first hinge rods 303 and second hinge rods 304 hinged together. The circulation device 3 also includes a base 305 fixedly connected to the hollow rod 1, a hollow telescopic rod 306 fixedly connected to the hollow rod 1, the other end of the hollow telescopic rod 306 fixedly connected to the second hinge block 302, and a connecting spring 307 fixedly connected to the side of the second hinge block 302 away from the hollow telescopic rod 306, with the other end of the connecting spring 307 fixedly connected to the hollow rod 1.

[0029] The hollow structure of the hollow telescopic rod 306 enables the material to be circulated and scattered through deformation, while also driving the movement of the structure.

[0030] The cyclic action device 3 also includes a wire harness wheel 308 fixedly connected to the outer wall of the hollow rod 1, and two drive motors 309 fixedly connected to the base 305. The output end of the drive motor 309 is fixedly connected to a rigid rack 310 through a coupling.

[0031] The circulation device 3 also includes an arc-shaped plate 311 fixedly connected to the rigid rack 310. A first filter plate 312 is fixedly connected to one side of the arc-shaped plate 311, and a second filter plate 313 is fixedly connected to the other side of the arc-shaped plate 311.

[0032] Example 2 further optimizes the water-improving microbial agent equipment provided in Example 1, specifically, as follows: Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 , Figure 9 , Figure 10 and Figure 11 As shown, the drive assembly 4 includes a winding wheel 401 rotatably connected to the base 305, a connecting rope 402 on the winding wheel 401, a support platform 403 fixedly connected to the second hinge block 302, a fixing block 404 fixedly connected to the bottom outer wall of the hollow rod 1, a rigid rack 310 rotatably connected to the fixing block 404 at one end away from the drive motor 309 via a bearing, and a winding rope 405 on the cable pulley 308.

[0033] The coil spring in the cable pulley 308 allows the cable 405 to be wound adaptively, meaning it can change size according to the movement of the rigid rack 310.

[0034] Furthermore, such as Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 , Figure 9 , Figure 10 and Figure 11 As shown, the feeding assembly 5 includes a main shaft 501 rotatably connected to the hinge of the first hinge rod 303 and the second hinge rod 304. A fixing plate 502 is fixedly connected to the bottom of the main shaft 501. A storage cylinder 503 is fixedly connected to the top outer wall of the first hinge rod 303. A auger 504 is rotatably connected in the storage cylinder 503. A transmission gear 505 is fixedly sleeved on the main shaft 501. The auger 504 is fixedly connected to the main shaft 501.

[0035] The rigid rack 310 drives the transmission gear 505 to rotate, thereby enabling the fixed plate 502 to rotate stably and simultaneously drive the upper auger 504 to rotate synchronously for material feeding.

[0036] The feeding assembly 5 also includes a rectangular groove 506 formed on the fixed plate 502, a first feeding nozzle 507 fixedly connected to the rectangular groove 506, and a second feeding nozzle 508 fixedly connected to the bottom of the second hinge block 302.

[0037] Example 3, the method of using the water-improving microbial agent equipment, specifically includes: Ⅰ. In the initial state, i.e. Figure 2 In this state, the device is installed on both sides of the hull via mounting plate 2 and mounting components, and is available in three usage scenarios; Scenario 1: A microbial agent for purification is applied to a small body of water. After installation, the motor inside the base 305 (not shown in the diagram, as the motor is existing technology and to avoid duplication) drives the winding reel 401 to rotate. The winding reel 401 transmits force through the connecting rope 402. The connecting rope 402 pulls the second hinge block 302 to slide on the hollow rod 1. During this movement, the second hinge block 302 stretches the connecting spring 307. At this time, the first hinge rod 303 and the second hinge rod 304 move around the hinge point, forming a... Figure 3 and Figure 4 The situation needs to be supplemented as follows: Figure 4 For ease of observation, the first filter plate 312 and the second filter plate 313 are hidden in the current state. (Note: This is not an error in the diagram.) It can be observed that during movement, the transmission gear 505 gradually meshes with the rigid rack 310, causing it to rotate. As the main shaft 501 at the bottom of the hinge point moves, it pushes aside weeds on the water surface while simultaneously rotating itself. During the rotation of the main shaft 501, the winch 504 outputs the material from the storage cylinder 503 through the first feed nozzle 507. It should be noted that the main shaft 501 has a hollow structure, and the storage cylinder 503 is connected to the first feed nozzle 507 via a feed pipe. The connection is not shown in the figure because the pipeline is existing technology. During the rotation of the fixed plate 502, the material can be spread and stirred more effectively. At the same time, the hollow telescopic rod 306 is compressed due to the movement of the second hinge block 302. It should be added that a feed hole is opened on the base 305. The feed hole is connected to the hollow part inside the hollow telescopic rod 306. The feed hole is located on one side of the base 305 and is connected to the outside through the hole in the middle of the winding wheel 401. Thus, the agent in the hollow part of the hollow telescopic rod 306 is output into the water body through the second feed nozzle 508. At the same time; Ⅱ. Scenario 2: In a large water area, during the movement of the ship, the water flow drives the fixed plate 502 to rotate. At this time, the fixed plate 502 can drive the auger 504 inside the storage cylinder 503 to rotate through the main shaft 501. At this time, no external driving energy is needed to spread the material in the storage cylinder 503. At the same time, the upper arc plate 311 and the first filter plate 312 and the second filter plate 313 on both sides block flying objects in the water area to prevent damage to the equipment body. In addition, the extended first hinge rod 303 and the second hinge rod 304 can enhance the stability of the arc plate 311 and improve the protective capability of the arc plate 311. Ⅲ. Scenario 3: High-altitude waters requiring climbing. Place the capsule-shaped microbial agent on the support platform 403, while holding the fixing plate 502 and passing it through the rectangular slot 506. At this time, the rectangular slot 506 and the fixing plate 502 act as handles. After the second hinge block 302 is moved to the position where it overlaps with the arc plate 311 by the rotation of the reel 401, place the microbial agent on the support platform 403. At this time, the arc plate 311 acts as a wind deflector to prevent the wind from both sides from interfering with the launch. The connecting spring 307 is stretched due to the movement of the second hinge block 302. After aiming at the water area, the connecting rope 402 can be cut. At this time, the second hinge block 302 is ejected to the top, and the microbial agent is launched into the designated water area. IV. When the hull is stationary, the drive motor 309 rotates, driving the rigid rack 310 to rotate via its output end. The rigid rack 310 drives the arc-shaped plate 311 to follow the movement, causing the two rigid racks 310 to come together to form a grab handle for retrieving floating objects. Figure 5 As shown; V. While the ship is in motion, and still in a state of... Figure 5 In the state shown, it should be noted that the aperture sizes of the first filter plate 312 and the second filter plate 313 are different. When the ship is moving, the water flows through the first filter plate 312 with a larger aperture and is then collected by the second filter plate 313 with a smaller aperture. This prevents larger impurities from entering and causing overload and equipment damage.

[0038] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0039] Obviously, the embodiments described above are merely some embodiments of the present invention, not all embodiments. The accompanying drawings show preferred embodiments of the present invention, but do not limit the patent scope of the present invention. The present invention can be implemented in many different forms; rather, these embodiments are provided to provide a more thorough and complete understanding of the disclosure of the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing specific embodiments, or make equivalent substitutions for some of the technical features. Any equivalent structures made using the content of this specification and drawings, directly or indirectly applied to other related technical fields, are similarly within the patent protection scope of this invention.

Claims

1. A method for preparing a microbial agent to improve water quality, characterized in that, Includes the following steps: Step 1: Selecting and culturing multifunctional strains: a. Select microbial strains with functions of degrading organic matter, denitrification, and phosphorus removal from the strain bank; b. Activate the selected bacterial strains using a culture medium containing carbon sources, nitrogen sources, and inorganic salts; c. Expand the culture in a constant temperature shaker to ensure the purity and activity of the strain; Step 2: Preparation of microbial fuel cell electrodes: a. Using carbon nanotube composite materials to fabricate the anode of a microbial fuel cell. Carbon nanotube composite materials have high conductivity and good biocompatibility, which can be used to support microbial attachment and biofilm formation. b. A cathode coated with a platinum catalyst is used to promote the oxygen reduction reaction; c. Pre-treat the electrodes, including cleaning, drying, and sterilization, to ensure sterile conditions; Step 3: Integrating microbial fuel cells and microbial agents: a. The cultured multifunctional microbial strains are evenly coated on the anode of the microbial fuel cell to ensure that the strains adhere firmly; b. Add the water sample to be treated into the microbial fuel cell device to ensure good contact between the anode, cathode, and electrolyte; c. Connect an external circuit to monitor and record the electrical output of the microbial fuel cell; Step 4: Fermentation and Optimization a. The device integrating the microbial fuel cell was placed in a constant temperature and humidity environment for 48 hours of fermentation; b. Optimize the degradation efficiency and power generation of the strain by adjusting fermentation conditions, including temperature, pH and dissolved oxygen; c. Monitor the power output of MFC in real time. When the power output reaches a stable level, fermentation is considered complete. Step 5: Preparation of microbial inoculants: a. After fermentation is complete, disconnect the external circuit of the MFC and remove the anode; b. Using aseptic techniques, scrape and rinse the attached microorganisms from the anode to form a uniform microbial suspension; c. Add protectants and stabilizers to the microbial suspension to ensure the survival rate and stability of the strain; d. Conduct quality testing on the microbial agent, including indicators of strain activity, purity, and quantity.

2. The method for preparing the microbial agent for improving water quality according to claim 1, characterized in that, In step three, the cultured multifunctional microbial strains are uniformly coated onto the anode of the microbial fuel cell. The specific coating process includes the following steps: Preparation of bacterial suspension: Take the cultured multifunctional microbial strain out of the culture medium, wash it with buffer and physiological saline to remove culture medium residue and impurities, and resuspend the strain in an appropriate amount of buffer and physiological saline to prepare a bacterial suspension. Anode surface treatment: Before coating the bacterial strain, the anode surface of the MFC needs to be subjected to plasma treatment, chemical etching and physical roughening treatment to increase the roughness of the anode surface, so as to increase the adhesion of the bacterial strain and provide more adhesion points for the bacterial strain. Coating the bacterial suspension: The prepared bacterial suspension is evenly coated on the anode surface of the MFC. The bacterial suspension is added drop by drop to the anode using a pipette to ensure that the bacterial suspension covers the entire anode surface and is evenly distributed.

3. The method for preparing the microbial agent for improving water quality according to claim 2, characterized in that, In step three, the cultured multifunctional microbial strains are uniformly coated onto the anode of the microbial fuel cell. The coating process further includes the following steps: Drying and Fixation: After coating the bacterial suspension, place the anode in a sterile environment and allow the bacterial suspension to dry naturally. During the drying process, the bacterial strain will gradually adhere to the anode surface. Use electric current stimulation and chemical cross-linking agent to fix the bacterial strain to ensure that the bacterial strain adheres firmly. Inspection and verification: After coating, the adhesion of the bacterial strain on the anode surface is inspected under a microscope, and the performance of the MFC is measured to verify whether the bacterial strain has successfully adhered.

4. A device for preparing a water-improving microbial agent, applicable to the method for preparing the water-improving microbial agent as described in claim 3, characterized in that... It includes a hollow rod (1) and a mounting plate (2) fixedly connected above it. A circulation device (3) is provided on the hollow rod (1), a drive assembly (4) is provided on the hollow rod (1), and a feeding assembly (5) is provided on the hollow rod (1).

5. The water-improving microbial agent equipment according to claim 4, characterized in that, The circulating device (3) includes a first hinge block (301) fixedly connected to the hollow part of the hollow rod (1), a second hinge block (302) slidably connected to the hollow rod (1) via a sliding block, two first hinge rods (303) hinged to the first hinge block (301), two second hinge rods (304) hinged to the second hinge block (302), the ends of the first hinge rods (303) and the second hinge rods (304) hinged together, the circulating device (3) also includes a base (305) fixedly connected to the hollow rod (1), a hollow telescopic rod (306) fixedly connected to the hollow rod (1), the other end of the hollow telescopic rod (306) fixedly connected to the second hinge block (302), a connecting spring (307) fixedly connected to the side of the second hinge block (302) away from the hollow telescopic rod (306), the other end of the connecting spring (307) fixedly connected to the hollow rod (1).

6. The water-improving microbial agent equipment according to claim 5, characterized in that, The circulation device (3) further includes a wire harness wheel (308) fixedly connected to the outer wall of the hollow rod (1). Two drive motors (309) are fixedly connected to the base (305). The output end of the drive motor (309) is fixedly connected to a rigid rack (310) through a coupling. The circulation device (3) further includes an arc plate (311) fixedly connected to the rigid rack (310). A first filter plate (312) is fixedly connected to one side of the arc plate (311), and a second filter plate (313) is fixedly connected to the other side of the arc plate (311).

7. The water-improving microbial agent equipment according to claim 6, characterized in that, The drive assembly (4) includes a winding wheel (401) rotatably connected to the base (305), a connecting rope (402) on the winding wheel (401), a support platform (403) fixedly connected to the second hinge block (302), a fixing block (404) fixedly connected to the bottom outer wall of the hollow rod (1), and the end of the rigid rack (310) away from the drive motor (309) rotatably connected to the fixing block (404) through a bearing. A winding rope (405) is provided on the winding wheel (308).

8. The water-improving microbial agent equipment according to claim 7, characterized in that, The feeding assembly (5) includes a main shaft (501) rotatably connected to the hinge of the first hinge rod (303) and the second hinge rod (304). A fixing plate (502) is fixedly connected to the bottom of the main shaft (501). A storage cylinder (503) is fixedly connected to the top outer wall of the first hinge rod (303). A auger (504) is rotatably connected in the storage cylinder (503). A transmission gear (505) is fixedly sleeved on the main shaft (501). The auger (504) is fixedly connected to the main shaft (501).

9. The water-improving microbial agent equipment according to claim 8, characterized in that, The feeding assembly (5) also includes a rectangular groove (506) opened on the fixed plate (502), a first feeding nozzle (507) is fixedly connected to the rectangular groove (506), and a second feeding nozzle (508) is fixedly connected to the bottom of the second hinge block (302).

10. A method of using a water-improving microbial agent purification device, wherein the water-improving microbial agent purification device as described in claim 9 is characterized in that, include: Ⅰ. In the initial state, it is installed on both sides of the hull by mounting plate (2) and mounting components, and is divided into three usage scenarios; Scenario 1: A microbial agent for purification is sprayed onto a small body of water. After installation, the motor inside the base (305) drives the winding wheel (401) to rotate. The winding wheel (401) transmits force through the connecting rope (402). At this time, the connecting rope (402) pulls the second hinge block (302) to slide on the hollow rod (1). During the movement, the second hinge block (302) stretches the connecting spring (307). At this time, the first hinge rod (303) and the second hinge rod (304) move around the hinge point. It can be observed that during the movement, the transmission gear (505) gradually meshes with the rigid rack (310). As a result, rotation occurs. During the movement of the main shaft (501) at the bottom of the hinge point, the fixed plate (502) is moved, which pushes aside the weeds on the water surface and rotates itself. During the rotation of the main shaft (501), the winch (504) outputs the material in the storage cylinder (503) through the first feed nozzle (507). During the rotation of the fixed plate (502), the material can be spread and stirred more effectively. At the same time, the hollow telescopic rod (306) is compressed due to the movement of the second hinge block (302). Thus, the agent in the hollow part of the hollow telescopic rod (306) is output into the water through the second feed nozzle (508). Ⅱ. Scenario 2: In a large water area, during the movement of the ship, the water flow drives the fixed plate (502) to rotate. At this time, the fixed plate (502) can drive the auger (504) inside the storage cylinder (503) to rotate through the main shaft (501). At this time, no external driving energy is needed to spread the material in the storage cylinder (503). At the same time, the upper part of the arc plate (311) and the first filter plate (312) and the second filter plate (313) on both sides block the flying objects in the water area to prevent damage to the equipment body. The first hinge rod (303) and the second hinge rod (304) can enhance the stability of the arc plate (311) and improve the protection capability of the arc plate (311). Ⅲ. Scenario 3: High-altitude waters that need to be climbed. Place the capsule-shaped microbial agent on the support platform (403), and hold the fixing plate (502) and pass it through the rectangular groove (506). At this time, the rectangular groove (506) and the fixing plate (502) act as handles. After the second hinge block (302) is moved to the overlapping part with the arc plate (311) by the operation of the reel (401), place the microbial agent on the support platform (403). At this time, the arc plate (311) acts as a wind deflector to prevent the wind on both sides from interfering with the launch. The connecting spring (307) is stretched due to the movement of the second hinge block (302). After aiming at the water area to be released, the connecting rope (402) can be cut. At this time, the second hinge block (302) is ejected to the top, and the microbial agent is launched into the designated water area. IV. When the hull is stationary, the drive motor (309) drives the rigid rack (310) to rotate through the output end. The rigid rack (310) drives the arc plate (311) to follow the movement, and the two rigid racks (310) come together to form a grab to salvage the floating object. V. When the ship is in motion, the water flows through the first filter plate (312) with a larger aperture and is then collected by the second filter plate (313) with a smaller aperture, thus preventing larger impurities from entering and causing overload and equipment damage.

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