Magnolia compound essential oil as well as preparation method and application thereof

Abstract

The invention provides magnolia denudata compound essential oil as well as a preparation method and application thereof, and the magnolia denudata compound essential oil comprises the following components in parts by mass: 30-40% of magnolia denudata essential oil, 15-20% of peppermint oil, 5-10% of fructus forsythiae oil, 8-10% of radix angelicae oil, 5-8% of radix saposhnikoviae oil, 5-8% of eucalyptus oil, 5-8% of ligusticum wallichii oil, 5-10% of lavender oil and 5-10% of agastache rugosus. The total percentage is 100%. The product provided by the invention can be applied to nasal products such as snorting, nasal stickers and nasal cleaning rods, relieving poor breath caused by acute nasosinusitis, nasal obstruction and headache caused by cold, relieving pressure and anxiety, refreshing and the like. The traditional Chinese medicine composition is extracted from pure plants, has no adverse reaction, can relieve poor breath caused by acute nasosinusitis and nasal obstruction and headache caused by cold at the same time, can relieve pressure and anxiety, and can refresh mind and restore consciousness.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a magnolia compound essential oil, its preparation method, and its application. Background Technology

[0002] Magnolia essential oil is a concentrated extract of volatile compounds from magnolia flower buds. Due to its unique aroma and potential medicinal properties, magnolia essential oil is widely used in the fragrance industry and aromatherapy.

[0003] The Pharmacopoeia of the People's Republic of China (National Pharmacopoeia Commission, 2020) defines the dried flower buds of Magnolia biondii, Magnolia denudata, or Magnolia sprengeri as Xin Yi. Xin Yi is a commonly used traditional Chinese medicine. It has a pungent taste and warm properties, and enters the lung and stomach meridians. It has the effects of dispelling wind-cold and clearing nasal passages, and is mainly used for sinusitis, headache, nasal congestion, and runny nose caused by wind-cold (State Administration of Traditional Chinese Medicine, 1999). Since Xin Yi has been developed and utilized as a traditional Chinese medicine since ancient times, it has extremely high medicinal value. Previous researchers have used various methods to determine and study the chemical components of Xin Yi, discovering that volatile oil content and magnolin are the main chemical components. At the same time, it has been proven that both can relieve symptoms such as rhinitis when used in vitro. The 2020 edition of the Chinese Pharmacopoeia (National Pharmacopoeia Commission, 2020) uses volatile oil content and magnolin content as the legal quality control indicators for Xin Yi.

[0004] Rhinitis, or nasal inflammation, is an inflammation of the nasal mucosa caused by viruses, bacteria, allergens, various physical and chemical factors, and certain systemic diseases. The main pathological changes in rhinitis include congestion, swelling, exudation, hyperplasia, atrophy, or necrosis of the nasal mucosa. Sinusitis, on the other hand, involves inflammation of one or more sinuses. It is a common disease affecting the population and impacting patients' quality of life. Some cases of rhinitis and sinusitis are notoriously difficult to treat, with some even considering them lifelong recurring illnesses. Although not directly life-threatening, the recurrent symptoms and long course of the disease often severely affect a person's life, work, and social interactions, causing considerable inconvenience.

[0005] This product was developed specifically to address the current poor air quality and the increasing prevalence of rhinitis among the population. Acute sinusitis is a common inflammatory disease of the sinus mucosa. Current clinical treatments primarily include antibiotics and hormones, with antibiotics being the primary treatment. However, due to the irrational use of antibiotics, drug resistance is widespread. Therefore, there is an urgent need to find new and effective drugs to treat acute sinusitis, reduce drug resistance, and improve patients' quality of life. Summary of the Invention

[0006] In order to overcome the technical problems existing in the prior art, especially the defects and shortcomings of the existing plant essential oil market where most magnolia essential oils are chemically synthesized, have single components, and poor medicinal effects, the purpose of this invention is to provide a natural magnolia compound essential oil that can not only effectively relieve breathing difficulties caused by acute sinusitis, but also alleviate nasal congestion and headaches caused by colds and flu, relax people, and relieve stress and anxiety; it can also be used to refresh the mind and improve mood.

[0007] To achieve the above-mentioned objectives, the present invention adopts the following technical solution:

[0008] According to a first aspect of the technical solution of the present invention, the present invention provides a magnolia compound essential oil, comprising the following components in parts by weight: 30%-40% magnolia essential oil, 15%-20% peppermint oil, 5%-10% forsythia oil, 8%-10% angelica dahurica oil, 5%-8% saposhnikovia divaricata oil, 5%-8% eucalyptus oil, 5%-8% chuanxiong oil, 5%-10% lavender oil, 5%-10% patchouli, and a base carrier oil to 100%.

[0009] Specifically, the mass fraction range described in this invention includes any value within that range.

[0010] Preferably, in some embodiments, the magnolia compound essential oil of the present invention comprises the following components by weight: 35% magnolia essential oil; 15% peppermint oil; 10% forsythia oil; 8% angelica oil; 7% saposhnikovia oil; 6% eucalyptus oil; 5% chuanxiong oil; 6% lavender oil; 5% patchouli oil; and a base carrier oil to 100%.

[0011] Preferably, in some embodiments, the magnolia compound essential oil of the present invention further comprises the following essential oils or a combination of essential oils and specified substances, wherein the specified substances are sweet almond oil, grapeseed oil, olive oil or coconut oil.

[0012] Preferably, in some embodiments, the magnolia compound essential oil of the present invention further comprises 3% lemon oil and / or 3% tea tree oil.

[0013] Furthermore, in some embodiments, the base carrier oil of the magnolia compound essential oil of the present invention is selected from the following essential oils or combinations thereof: sweet almond oil, grapeseed oil, olive oil or coconut oil, etc.

[0014] According to a second aspect of the technical solution of the present invention, the present invention provides a method for preparing magnolia essential oil, which includes the following steps:

[0015] Step S1: Collect magnolia buds that are about to open, wash them and dry them until the moisture content is between 5% and 10%;

[0016] Step S2: Grind the dried magnolia flower buds from Step 1 and sieve them. Soak them in 30% to 70% ethanol (50 to 100 times the mass of magnolia flowers) to obtain a solid-liquid mixture. Place the mixture at a low temperature of 4°C to 8°C for 10 to 24 hours.

[0017] Step S3: Filter the mixture after step 2 to remove residue, evaporate the organic solvent using a rotary distillation apparatus, and retain the concentrated extract;

[0018] Step S4: Purify the concentrated extract obtained in step 3 by crystallization, chromatographic separation or other separation techniques to obtain the final product.

[0019] Preferably, the placement time in step S2 is 14 hours.

[0020] According to a third aspect of the technical solution of the present invention, the present invention provides the application of magnolia compound essential oil in nasal products such as nasal inhalers, nasal strips and nasal cleansing sticks.

[0021] According to a fourth aspect of the technical solution of the present invention, the present invention provides the application of magnolia compound essential oil in relieving breathing difficulties caused by acute sinusitis and nasal congestion and headache caused by colds.

[0022] According to a fifth aspect of the technical solution of the present invention, the present invention provides an application of magnolia compound essential oil in relieving stress and anxiety.

[0023] According to a sixth aspect of the present invention, the present invention provides the application of magnolia compound essential oil in a product for refreshing and invigorating the mind.

[0024] Compared with the prior art, the present invention has the following beneficial technical effects:

[0025] 1. The magnolia compound essential oil provided by this invention uses 8 kinds of essential oils in a certain proportion to work together. It is a pure plant extract with no adverse reactions. It can simultaneously relieve breathing difficulties caused by acute sinusitis and nasal congestion and headache caused by colds, relieve stress and anxiety, and refresh the mind.

[0026] 2. The method for preparing magnolia essential oil provided by this invention is simple, and the extraction is carried out using magnolia flower buds, resulting in a higher content of effective components in the obtained essential oil and better effects. Detailed Implementation

[0027] To make the technical problems solved by this invention, the technical solutions adopted, and the beneficial effects obtained clearer, the invention will be further described in detail below with reference to specific embodiments. The specific embodiments described herein are for illustrative purposes only and are not intended to constitute any limitation on the invention. Unless otherwise defined, all terms used herein have the same meaning as commonly used in the field to which this invention pertains.

[0028] The following specific embodiments are provided to help understand the present invention. However, it should be understood that the embodiments and test examples listed in the present invention are only for illustrating the present invention and do not constitute any limitation. The actual scope of protection of the present invention is set forth in the claims.

[0029] This invention provides a magnolia compound essential oil, comprising the following components by weight: 30%–40% magnolia essential oil, 15%–20% peppermint oil, 5%–10% forsythia oil, 8%–10% angelica dahurica oil, 5%–8% saposhnikovia divaricata oil, 5%–8% eucalyptus oil, 5%–8% chuanxiong oil, 5%–10% lavender oil, and 5%–10% patchouli oil, with a base carrier oil added to 100%. The weight range described in this invention includes any value within this range.

[0030] Preferably, the magnolia compound essential oil of the present invention comprises the following components by weight: 35% magnolia essential oil; 15% peppermint oil; 10% forsythia oil; 8% angelica oil; 7% saposhnikovia oil; 6% eucalyptus oil; 5% chuanxiong oil; 6% lavender oil; 5% patchouli oil; and a base carrier oil to 100%. Preferably, the magnolia compound essential oil of the present invention further comprises the following essential oils or combinations of essential oils with specified substances, wherein the specified substances are sweet almond oil, grapeseed oil, olive oil, or coconut oil. Preferably, the magnolia compound essential oil of the present invention further comprises 3% lemon oil and / or 3% tea tree oil.

[0031] Furthermore, the base carrier oil of the magnolia compound essential oil of the present invention is selected from the following essential oils or combinations thereof: sweet almond oil, grape seed oil, olive oil or coconut oil, etc.

[0032] Furthermore, this invention provides a method for preparing magnolia essential oil, which includes the following steps:

[0033] Step S1: Collect magnolia buds that are about to open, wash them and dry them until the moisture content is between 5% and 10%;

[0034] Step S2: Grind the dried magnolia flower buds from Step 1 and sieve them. Soak them in 30% to 70% ethanol (50 to 100 times the mass of magnolia flowers) to obtain a solid-liquid mixture. Place the mixture at a low temperature of 4°C to 8°C for 10 to 24 hours.

[0035] Step S3: Filter the mixture after step 2 to remove residue, evaporate the organic solvent using a rotary distillation apparatus, and retain the concentrated extract;

[0036] Step S4: Purify the concentrated extract obtained in step 3 by crystallization, chromatographic separation or other separation techniques to obtain the final product.

[0037] Preferably, the placement time in step S2 is 14 hours.

[0038] Verification has shown that the magnolia compound essential oil provided by this invention can be used in nasal products such as nasal inhalers, nasal strips, and nasal cleansing sticks. Furthermore, the magnolia compound essential oil can relieve breathing difficulties caused by acute sinusitis and nasal congestion and headaches caused by colds. In addition, the magnolia compound essential oil can relieve stress and anxiety and refresh the mind.

[0039] The present invention will be further described below with reference to specific embodiments.

[0040] Example 1: Magnolia essential oil was prepared by the following method:

[0041] Step S1: Collect magnolia buds that are about to open, wash and dry them until the moisture content is between 5% and 10%. After removing impurities, rinse the collected buds with running water, then soak them in a clean water tank for 1 to 5 hours to remove stubborn stains. If necessary, use a solvent to remove stubborn stains or gels; this process must be repeated afterward. Heat the washed buds over a low flame (temperature not exceeding 500℃) until there are no water droplets or watermarks on the surface, and some areas of the buds turn slightly yellow.

[0042] Step S2: After drying the magnolia flower buds in Step S1, grind them and sieve them. Soak them in an organic solvent to obtain a solid-liquid mixture. Place the solid-liquid mixture at a low temperature of 4℃~8℃ for 10 hours~24 hours. The organic solvent is preferably 50%~80% ethanol.

[0043] The magnolia buds dried in step S1 are ground and sieved, and then crushed using a spiral mill. After crushing, magnolia bud particles with a particle size of less than 1 cm are obtained, preferably particles with a particle size of less than 0.5 cm, and most preferably particles with a particle size of less than 0.3 cm.

[0044] The magnolia bud particles are preferably soaked in 75% ethanol at a mass ratio of 60 to 80 times to obtain a solid-liquid mixture. The solid-liquid mixture is then placed at a low temperature of 4°C to 8°C for 12 to 14 hours.

[0045] Step S3: Filter the mixture from Step S2 to remove residue, evaporate the organic solvent using a distillation apparatus, and retain the concentrated extract; preferably, place the solid-liquid mixture obtained after soaking in a cool room at a temperature of 18°C ​​to 25°C, evaporate the organic solvent using a rotary evaporator, and allow it to cool naturally. The cool room must be kept well-ventilated and clean.

[0046] Step S4: The concentrated extract obtained in step S3 is purified by separation techniques such as crystallization and chromatography. The concentrated extract is then finely ground into a powder using a mechanical steel mill and passed through a 600-mesh sieve to obtain a mixed fine powder for later use. The grinding speed of the mechanical steel mill is preferably 2500 rpm to 5000 rpm, and in one embodiment, 4000 rpm is preferred.

[0047] Finally, the genistein compound essential oil was prepared according to the proportions of each component in the genistein compound essential oil by mass.

[0048] The purification steps of the above chromatographic separation technique are as follows:

[0049] Step W1: Dissolve the magnolia essential oil extract in pure ethanol to obtain the separated sample;

[0050] Step W2: Insert the sample into the reversed-phase chromatography column using a syringe or similar device;

[0051] Step W3: Use pure methanol as the elution solvent;

[0052] Step W4: Prepare a mobile phase with a methanol:water ratio of 1:5;

[0053] Step W5: The sample is separated by passing it through a chromatographic column;

[0054] Step W6: Collect the magnolin elution peak based on the relative retention times of the separated components;

[0055] Step W7: Remove the elution solvent by concentration to obtain the purified magnolin product;

[0056] Step W8: Perform mass spectrometry and infrared spectroscopy analysis on the purified magnolin product to determine the structure and purity of the magnolin;

[0057] Step W9: Dissolve the purified magnolin in 100% ethanol, stir to allow the magnolin to gradually crystallize from the solution, filter with filter paper, wash the crystals 2-3 times with 100% ethanol, dry under vacuum, and collect the purified magnolin.

[0058] Example 2: Preparation of Magnolia Compound Essential Oil:

[0059] Combine 35 parts magnolia essential oil, 15 parts peppermint oil, 10 parts forsythia oil, 8 parts angelica oil, 7 parts saposhnikovia oil, 6 parts eucalyptus oil, 5 parts chuanxiong oil, 6 parts lavender oil, and 5 parts patchouli oil in a container. Add sweet almond oil to a total of 100 parts and stir thoroughly to ensure a complete mixture. Pour the mixed essential oil into a 10ml glass bottle with a lid and seal it.

[0060] Comparative Example 1: Preparation of blank carrier oil.

[0061] Add sweet almond oil to a 10ml glass bottle with a lid, then seal the bottle.

[0062] Comparative Example 2: Preparation of compound essential oils without magnolia essential oil.

[0063] Add 15 parts peppermint oil, 10 parts forsythia oil, 8 parts angelica oil, 7 parts saposhnikovia oil, 6 parts eucalyptus oil, 5 parts chuanxiong oil, 6 parts lavender oil, and 5 parts patchouli oil to a container. Add sweet almond oil to 100 parts and stir thoroughly to ensure a complete mixture. Pour the mixed essential oil into a 10ml glass bottle with a lid and seal it.

[0064] Comparative Example 3: Preparation of compound essential oils with low content of magnolia essential oil.

[0065] Combine 20 parts magnolia essential oil, 15 parts peppermint oil, 10 parts forsythia oil, 8 parts angelica oil, 7 parts saposhnikovia oil, 6 parts eucalyptus oil, 5 parts chuanxiong oil, 6 parts lavender oil, and 5 parts patchouli oil in a container. Add sweet almond oil to a total of 100 parts and stir thoroughly to ensure a complete mixture. Pour the mixed essential oil into a 10ml glass bottle with a lid and seal it.

[0066] Test Example 1: A rat rhinitis model was constructed for testing.

[0067] A rat rhinitis model was constructed for testing. Twenty rats were randomly divided into four groups of five each.

[0068] Each group of rats applied the compound essential oils of Example 2, Comparative Example 1, Comparative Example 2, and Comparative Example 3 to their noses using cotton swabs three times daily. The experiment lasted for 7 days. Afterwards, nasal mucosal tissue samples were collected from the rats and examined under a light microscope for degeneration and necrosis of the mucosal epithelial cells, and for congestion, edema, and inflammatory cell infiltration in the submucosa. The degree of damage to the nasal mucosa and various tissues was scored within a range of 1 to 10, and the average value was taken. The results are shown in the table below:

[0069] Group score Example 2 1.6 Comparative Example 1 6.6 Comparative Example 2 4.8 Comparative Example 3 2.8

[0070] Test Example 2: Volunteers with symptoms of cold, nasal congestion, and headache were selected for the test.

[0071] Thirty volunteers (20 women and 10 men) with symptoms of cold, nasal congestion, and headache were selected. All volunteers were over 18 years old and had no history of essential oil allergies. Each volunteer was given one bottle of the essential oil prepared in Example 2. When experiencing nasal congestion and headache, volunteers applied a few drops of the essential oil to their wrists, rubbed them together to warm the oil, and inhaled deeply, or applied a few drops to a handkerchief and gently inhaled. This was continued for seven days. Volunteer feedback indicated that the use significantly reduced nasal congestion and headache symptoms, with an effectiveness rate exceeding 95%.

[0072] Ten participants each from high school, university, and graduate programs were selected, aged 18-28, in good health, with no major stressful events in the past three months, no mental illness, and no history of essential oil allergies. They abstained from spicy foods, alcohol, and caffeinated beverages for one week during the test. When experiencing anxiety or stress, participants applied a few drops of essential oil to their wrists, rubbed them together to warm them, and inhaled deeply, or applied a few drops to a handkerchief and gently inhaled. The experiment lasted one month. User feedback indicated that the essential oils effectively relieved anxiety or stress without any discomfort, with an effectiveness rate exceeding 96%. Only one 28-year-old male graduate student did not experience significant effects; the rest experienced relief or cure of their anxiety or stress.

[0073] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solutions of the present invention within the scope of the inventive concept. These simple modifications all fall within the protection scope of the present invention.

[0074] It should also be noted that the various specific technical features described in the above embodiments can be combined in any way without contradiction. To avoid unnecessary repetition, the various possible combinations will not be described separately. Furthermore, different embodiments of the present invention can also be combined arbitrarily, as long as they do not violate the spirit of the present invention, and should also be considered as the content disclosed by the present invention.

Claims

1. A magnolia compound essential oil, characterized in that, It includes the following components by weight: 30%–40% magnolia essential oil, 15%–20% peppermint oil, 5%–10% forsythia oil, 8%–10% angelica dahurica oil, 5%–8% saposhnikovia divaricata oil, 5%–8% eucalyptus oil, 5%–8% chuanxiong oil, 5%–10% lavender oil, and 5%–10% patchouli oil, with the base carrier oil added to 100%.

2. The magnolia compound essential oil according to claim 1, characterized in that, It comprises the following components by weight: 35% magnolia essential oil; 15% peppermint oil; 10% forsythia oil; 8% angelica oil; 7% saposhnikovia oil; 6% eucalyptus oil; 5% chuanxiong oil; 6% lavender oil; 5% patchouli oil; and base carrier oil to 100%.

3. The magnolia compound essential oil according to any one of claims 1-2, characterized in that, It also contains the following essential oils or combinations thereof: lemon oil, chamomile oil, jasmine oil, rose oil, or tea tree oil.

4. The magnolia compound essential oil according to claim 2, characterized in that, It also contains 3% lemon oil and / or 3% tea tree oil.

5. The magnolia compound essential oil according to any one of claims 1-2, characterized in that, The base carrier oil is selected from the following essential oils or a combination of essential oils and a specified substance, wherein the specified substance is sweet almond oil, grapeseed oil, olive oil or coconut oil.

6. The magnolia compound essential oil according to claim 1, characterized in that, The magnolia essential oil mentioned above is prepared by the following method: Step S1: Collect magnolia buds that are about to open, wash them and dry them until the moisture content is between 5% and 10%; Step S2: Grind the dried magnolia buds from Step 1 and sieve them. Soak them in 30% to 70% ethanol (50 to 100 times the mass of magnolia flowers) to obtain a mixture. Place the mixture at a low temperature of 4 to 8°C for 14 hours. Step S3: Filter the mixture after step 2 to remove residue, evaporate the organic solvent using a rotary evaporator, and retain the concentrated extract; Step S4: Purify the concentrated extract obtained in step 3 by crystallization, chromatographic separation or other separation techniques to obtain the final product.

7. The method for preparing magnolia compound essential oil according to claim 6, characterized in that, The placement time in step 2 is 14 hours.

8. The application of the magnolia compound essential oil according to any one of claims 1-5 in nasal products such as nasal inhalers, nasal strips, and nasal cleansing sticks.

9. The use of the magnolia compound essential oil according to any one of claims 1-5 in relieving nasal congestion and headache caused by colds.

10. The application of the magnolia compound essential oil according to any one of claims 1-5 in relieving stress and anxiety.