Preparation process of zanthoxylum bungeanum root rot resistant microbial organic fertilizer

Abstract

The invention provides a preparation process of a zanthoxylum bungeanum root rot resistant microbial organic fertilizer. Relates to the technical field of agricultural microbial organic fertilizer preparation, and comprises the following steps: extracting strains from rhizosphere soil and diseased plant roots of zanthoxylum bungeanum maxim, screening microorganisms with antagonistic activity on zanthoxylum bungeanum maxim root rot pathogenic bacteria, and compounding the microorganisms into a composite strain according to a predetermined ratio. According to the preparation process of the zanthoxylum bungeanum root rot resistant microbial organic fertilizer, strains with high antagonistic activity, such as bacillus, pseudomonas fluorescens and trichoderma, are screened and compounded, so that a strong inhibition effect on zanthoxylum bungeanum root rot pathogenic bacteria is ensured, and the root rot resistant effect is improved. The ratio of the organic matrix to the auxiliary materials is optimized, the strain can better adapt to and proliferate after colonization according to the requirement of the pepper root system, and the problems that in the prior art, the survival rate is low, and colonization is difficult are solved. The temperature, the humidity, the ventilation capacity and the pH value are regulated and controlled through the intelligently controlled solid state fermentation device, it is ensured that strains proliferate in the optimal environment, and the number and the activity of thalli are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of agricultural microbial organic fertilizer preparation, in particular to a preparation process of a Zanthoxylum bungeanum root rot resistant microbial organic fertilizer. BACKGROUND

[0002] At present, the main methods for preventing and treating Zanthoxylum bungeanum root rot include chemical pesticide spraying and soil or seed treatment. Chemical pesticides have a short-term inhibitory effect on soil-borne pathogens such as Fusarium and Pseudomonas, but the effect on deep root systems is limited, and long-term use can easily promote the development of drug resistance in pathogens, and pesticide residues pose a risk to the ecological environment and fruit safety. With the popularization of the concept of green planting, organic fertilizers and biological fertilizers have gradually attracted attention due to their environmental protection and efficiency characteristics, and a number of microbial organic fertilizers containing antagonistic bacteria or growth-promoting bacteria have appeared on the market, aiming to inhibit pathogens and improve soil structure and nutrient supply by constructing a protective rhizosphere microbial community.

[0003] However, the prior art still has significant deficiencies: most products have single strain composition or low activity, and the antagonistic effect on Zanthoxylum bungeanum root rot pathogens is unstable; the ratio of organic matrix and auxiliary materials is not optimized for the root system requirements of Zanthoxylum bungeanum, resulting in low survival rate and poor sustained effect of the inoculated beneficial bacteria after colonization. SUMMARY

[0004] In view of the deficiencies of the prior art, the present application provides a preparation process of a Zanthoxylum bungeanum root rot resistant microbial organic fertilizer, which solves the problems of single strain composition or low activity in the prior art, unstable antagonistic effect on pathogens, and the ratio of organic matrix and auxiliary materials not being optimized for the root system requirements of Zanthoxylum bungeanum, resulting in low survival rate and difficulty in colonization of the inoculated beneficial bacteria.

[0005] To achieve the above purpose, the present application is implemented by the following technical solution: a preparation process of a Zanthoxylum bungeanum root rot resistant microbial organic fertilizer, comprising: S1. Extracting strains from Zanthoxylum bungeanum rhizosphere soil and diseased root, screening out microorganisms with antagonistic activity against Zanthoxylum bungeanum root rot pathogens, and compounding the microorganisms into a composite strain according to a predetermined ratio; S2. Mixing a fully decomposed organic matrix with auxiliary materials in a predetermined ratio as a solid-state fermentation substrate; S3. Inoculating the composite strain into the fermentation substrate to form a pre-culture substrate, and performing preliminary pre-culture according to a set inoculation amount to promote adaptive proliferation of the strain in the substrate; S4. Placing the pre-culture substrate in a solid-state fermentation device with online multi-parameter monitoring and intelligent control, and through linkage adjustment of temperature, aeration rate, humidity and pH value, performing multi-stage fine fermentation to obtain fermented material; S5. Dehydrating, granulating and drying the fermented material to form a microbial organic fertilizer product.

[0006] Preferably, the complex bacterial species is compounded by bacillus, pseudomonas fluorescens and trichoderma in a mass ratio of 1:1:1.

[0007] Preferably, the pre-culture condition is temperature 30-35 DEG C, relative humidity 50-60%, time 24-48h.

[0008] Preferably, the solid state fermentation device controls the proliferation of bacterial cells by improving the Logistic model, and the model formula is as follows: Wherein, is the bacterial concentration after fermentation, is the initial bacterial concentration at the end of pre-culture, is the maximum bacterial concentration that can be reached during fermentation, is the maximum specific growth rate, is the fermentation time.

[0009] Preferably, the pH value range is 6.8-7.2, and the solid state fermentation device maintains the pH value in the range by automatically adding buffer.

[0010] Preferably, the particle size of the fermentation material after the granulation treatment is 3-5mm, and the drying treatment is drying at 50-60 DEG C for 2-4h.

[0011] The application provides a preparation process of Zanthoxylum bungeanum anti-root rot microbial organic fertilizer. The preparation process of Zanthoxylum bungeanum anti-root rot microbial organic fertilizer, by screening and compounding bacterial species with high antagonistic activity, such as bacillus, pseudomonas fluorescens and trichoderma, ensures strong inhibition effect on Zanthoxylum bungeanum root rot pathogen and improves the effect of anti-root rot. The ratio of organic matrix and auxiliary materials is optimized, and the demand of Zanthoxylum bungeanum root system is met, so that the bacterial species can better adapt and proliferate after colonization, solving the problems of low survival rate and difficult colonization in traditional technology. Through the intelligent control of the solid state fermentation device, the temperature, humidity, ventilation and pH value are adjusted, so that the bacterial species can proliferate in the optimal environment, and the number and activity of bacterial cells are improved.

[0012] ​This invention also precisely controls key parameters during the fermentation process of Sichuan pepper root rot-resistant microbial organic fertilizer, such as pH and temperature, significantly improving the quality and effectiveness of the microbial organic fertilizer. During fermentation, the pH is strictly controlled within the range of 6.8 to 7.2 to maintain high activity and stability of the microorganisms, achieved through automatic addition of buffer solution in a solid-state fermentation device. The temperature is precisely controlled between 30°C and 35°C to promote rapid growth and metabolism of the microorganisms, improving fermentation efficiency. The fermented material is granulated to a size of 3 to 5 mm, enhancing the application effect of the microbial organic fertilizer and making it easier for plants to absorb, store, and transport. In the drying stage, the material is dried at 50°C to 60°C for 2 to 4 hours, reducing the moisture content to ≤12%, extending the product's shelf life while maintaining microbial activity. These strict process control steps ensure the high efficiency and reliability of the microbial organic fertilizer, providing an effective method for disease control in Sichuan pepper cultivation. Attached Figure Description

[0013] Figure 1 This is a flowchart of the preparation process of the present invention; Figure 2 This is a schematic diagram of the intelligent fermentation control of the present invention. Detailed Implementation

[0014] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0015] Example 1 like Figures 1-2 As shown in the figure, the present invention provides a process for preparing a microbial organic fertilizer for preventing root rot of Sichuan pepper, including the following steps: S1. Extract strains from the rhizosphere soil and roots of diseased plants of Sichuan pepper, screen out microorganisms with antagonistic activity against the pathogen of Sichuan pepper root rot, and compound the microorganisms into a compound strain according to a predetermined ratio. The compound strain is composed of Bacillus, Pseudomonas fluorescens and Trichoderma in a mass ratio of 1:1:1.

[0016] S2. The fully decomposed organic substrate and auxiliary materials are mixed in a predetermined ratio to form a solid fermentation substrate. The organic substrate is a mixture of livestock and poultry manure, organic straw, oil residue and auxiliary materials in a mass ratio of 4:3:2:1, and the moisture content of the organic substrate is 60%.

[0017] S3. Inoculate the compound strain into the fermentation substrate to form a pre-culture substrate, and carry out preliminary pre-culture according to the set inoculation amount. The pre-culture conditions are: temperature 30℃, relative humidity 50%, and time 24.

[0018] S4. Placing the pre-culture medium into a solid-state fermentation device with online multi-parameter monitoring and intelligent control, through the linkage adjustment of temperature, aeration, humidity and pH value, through multi-stage fine fermentation and obtaining the fermentation material, the pH value range is 6.8, the solid-state fermentation device maintains the pH value in the range by automatically adding buffer, the solid-state fermentation device controls the proliferation of bacterial cells by improving the Logistic model, the model formula is as follows: wherein, is the bacterial cell concentration after fermentation, is the initial bacterial cell concentration at the end of pre-culture, is the maximum bacterial cell concentration that can be reached during fermentation, is the maximum specific growth rate, is the fermentation time.

[0019] The aeration is dynamically adjusted according to the oxygen transfer model, and the model formula is as follows: wherein, OTR is the oxygen transfer rate, is the volumetric mass transfer coefficient, is the air saturation dissolved oxygen concentration, is the actual dissolved oxygen concentration of the culture medium.

[0020] S5. Dehydrating, granulating and drying the fermentation material to form a microbial organic fertilizer product, the water content of the fermentation material after the dehydration treatment is ≤12%, the particle size of the fermentation material after the granulation treatment is 3mm, and the drying treatment is drying at 50℃ for 2h.

[0021] Example Two Based on Example One, this example carries out more in-depth processing by higher temperature, humidity and fermentation time to improve the activity of the bacterial strain and the effect of the final fertilizer, including the following steps: S1. Extracting bacterial strains from the rhizosphere soil and root of diseased plants of Zanthoxylum bungeanum, screening out microorganisms with antagonistic activity against the pathogenic fungi of Zanthoxylum bungeanum root rot, and compounding the microorganisms into a composite bacterial strain according to a predetermined ratio, the composite bacterial strain is compounded by Bacillus, Pseudomonas fluorescens and Trichoderma in a mass ratio of 1:1:1.

[0022] S2. Mixing fully decomposed organic matrix and auxiliary materials according to a predetermined ratio as a solid-state fermentation matrix, the organic matrix is mixed by livestock and poultry manure, organic straw, oil residue and auxiliary materials in a mass ratio of 4:3:2:1, and the water content of the organic matrix is 70%.

[0023] ​S3. Inoculate the complex strain into the fermentation substrate to form a pre-culture substrate, and perform preliminary pre-culture at a set inoculation amount. The pre-culture conditions are a temperature of 35°C, a relative humidity of 60%, and a time of 48h.

[0024] S4. Place the pre-culture substrate in a solid-state fermentation device with online multi-parameter monitoring and intelligent control. Through linkage adjustment of temperature, ventilation volume, humidity, and pH value, multi-stage fine fermentation is performed to obtain fermented material. The pH value range is 7.2. The solid-state fermentation device maintains the pH value within the range by automatically adding a buffer solution. The solid-state fermentation device controls the proliferation of the bacterial cells by improving the Logistic model. The model formula is as follows: wherein, is the bacterial cell concentration after fermentation, is the initial bacterial cell concentration at the end of pre-culture, is the maximum bacterial cell concentration that can be reached during fermentation, is the maximum specific growth rate, is the fermentation time.

[0025] The ventilation volume is dynamically adjusted according to the oxygen transfer model. The model formula is as follows: wherein, OTR is the oxygen transfer rate, is the volumetric mass transfer coefficient, is the air saturation dissolved oxygen concentration, is the actual dissolved oxygen concentration of the culture medium.

[0026] S5. Perform dehydration, granulation, and drying treatment on the fermented material to form a microbial organic fertilizer product. The water content of the fermented material after dehydration treatment is ≤12%. The particle size of the fermented material after granulation treatment is 5mm. The drying treatment is drying at 60°C for 4h.

[0027] Example Three Different from Example One, this example provides a preparation process for Zanthoxylum bungeanum anti-root rot microbial organic fertilizer. The substrate material, auxiliary materials, and production scale are adjusted to adapt to the needs of different planting modes. The process includes the following steps: 1. Strain extraction and selection Microorganisms are extracted from the rhizosphere soil and diseased root of organic Zanthoxylum bungeanum cultivation area. Three kinds of high-efficiency anti-root rot strains are mainly selected, such as Bacillus, Pseudomonas fluorescens, and Trichoderma.

[0028] Strain compounding ​The three microorganisms are compounded according to a mass ratio of 1:1:1 to form a compound microbial strain. In each gram of the compound microbial strain, the number of live bacteria of Bacillus, Pseudomonas fluorescens and Trichoderma is required to be: Bacillus: 1x10 8 CFU / g, Pseudomonas fluorescens: 1x10 8 CFU / g, and Trichoderma: 5x10 7 CFU / g.

[0029] Matrix and auxiliary material optimization The matrix meeting the organic agricultural standards is selected, such as humus soil, straw and organic compost. The matrix is mixed according to a mass ratio of 5:2:2:1 of humus soil, straw, organic compost and auxiliary material. The matrix is used to treat 1 ton of compound microbial strain to ensure that each 100 kg of matrix can obtain sufficient microbial colonization. The auxiliary material is selected to be organic rice bran and soybean meal which are rich in nitrogen organic matter.

[0030] Fermentation process The compound microbial strain is uniformly inoculated into the organic matrix with a temperature of 30°C, a humidity of 50% relative humidity, a pH value of 6.8 and a moderate oxygen supply. The pre-culture time is 24 hours and the overall fermentation time is 5 days to ensure that the initial inoculation amount of the microbial strain in each ton of matrix is 1x10 9 CFU / g. In the intelligent solid-state fermentation device, the temperature, humidity, ventilation and pH value are adjusted in real time through multi-stage fine fermentation to ensure the optimal fermentation process. The online monitoring system is used to automatically adjust the parameters.

[0031] Drying and granulation After the fermentation is completed, a low-temperature drying process is adopted with a drying temperature of 50°C and a drying time of 2 hours. The fermented material is granulated by a disc granulator with a particle size of 3 mm to ensure that the fertilizer particle size is moderate, facilitating application and dissemination.

[0032] Final effect The microbial organic fertilizer contains 1x10 8 CFU / g of live bacteria per kilogram. After the microbial organic fertilizer is used in the organic pepper cultivation, the incidence of root rot is reduced by more than 85%, significantly improving the yield and quality of organic pepper.

[0033] Example four This example is directed to S4. The pre-culture matrix is placed in a solid-state fermentation device with online multi-parameter monitoring and intelligent control. The dynamic control strategy of the Logistic model is improved to ensure efficient proliferation of the bacterial body and maintain high activity. The steps include: Intelligent control group Parameter initialization concentration: =0.5、 .

[0034] Maximum cell concentration: During the fermentation process, the cell concentration reaches a predetermined maximum concentration.

[0035] Multi-stage regulation: 0-12h preferentially increase the cell concentration, the ventilation amount is set to 0.8L / min, the temperature is 32℃, 12-24h balance metabolism, the pH is stabilized at 7.0, the humidity is adjusted to 55%, the ventilation amount is continuously maintained according to the growth of the cells, ensuring sufficient oxygen supply during the metabolism process, 24-36h inhibit the growth of miscellaneous bacteria, briefly raise the temperature to 35℃, and real-time monitor the cell concentration. When the measured cell concentration reaches 95% or the fermentation time is greater than or equal to 36h, the fermentation process is terminated.

[0036] Control fixed parameter group Fixed pH=7.0, temperature 32℃, ventilation amount 0.5L / min.

[0037] Verification index and method Cell concentration change index: The change of cell concentration is monitored by online or interval sampling, the concentration at each time point is recorded, and the optical density measurement method is used to evaluate the change of cell concentration through optical density detection.

[0038] pH value change index: The pH value fluctuation during the fermentation process is monitored in real time by using an online pH meter to ensure that the pH values of the intelligent control group and the control group are maintained within the set range, and whether there is deviation is observed and adjusted.

[0039] Temperature and humidity fluctuation: The control accuracy of temperature and humidity during the fermentation process is ensured to adjust the experimental intelligent control group according to the predetermined dynamic adjustment scheme.

[0040] Fermentation time and cell activity index: The specific growth rate of the cells is determined by the relationship between the fermentation time and the cell activity.

[0041] Miscellaneous bacteria inhibition effect index: The effect of the experimental group in inhibiting miscellaneous bacteria is determined by regularly checking the fermentation materials to confirm that the growth of miscellaneous bacteria is inhibited.

[0042] Quality and yield of fermentation product index: The quality and yield of the fermentation product are determined by chemical and physical analysis to evaluate the quality and yield of the fermentation product.

[0043] Strain purity index: Whether the strain purity reaches the expected value at the end of fermentation is determined by using microbial detection technology to detect whether there are residual miscellaneous bacteria, and ensuring that the final product mainly contains the target strain.

[0044] Indicators Detection method Experimental group VS control group results Bacterial peak concentration Bacterial concentration measured every hour Increased by 20% to 30% Fermentation efficiency Time required to reach peak concentration Shortened by 25% to 40% Strain activity preservation rate CFU count after fermentation Survival rate > 90% Energy consumption ratio Energy consumption per unit output Reduced by 15% to 20% Bacterial concentration changes Online or interval sampling, optical density measurement Experimental group concentration increased faster pH changes Real-time monitoring by online pH meter Intelligent control group pH more stable Temperature and humidity fluctuations Temperature and humidity control accuracy during fermentation Experimental group control more accurate Fermentation time and bacterial activity Relationship between fermentation time and bacterial activity Experimental group activity higher Effect of inhibiting miscellaneous bacteria Regularly check fermentation materials Experimental group miscellaneous bacteria growth inhibited Strain concentration Microbial detection technology Experimental group strain concentration higher Time Experimental group bacterial concentration Control group bacterial concentration 12 1.2 0.9 24 2.1 1.5 36 Terminated at 2.4 Continue fermentation at 1.8 Conclusion: The experimental group improved the Logistic model to predict real-time optimization of environmental conditions, reaching the peak of bacterial concentration 12 hours earlier and increasing the final concentration by 33%. The short-term temperature increase strategy can specifically inhibit the growth of unwanted bacteria without affecting the target bacteria, thereby improving fermentation efficiency and bacterial activity while reducing energy consumption. These results indicate that dynamic control strategies have significant advantages in solid-state fermentation processes, enabling more effective control of the fermentation process and improving product quality and yield.

[0045] While embodiments of the application have been shown and described, it is to be understood that the application is not limited to the details of the embodiments described, since modifications can be made by those skilled in the art, without departing from the spirit and scope of the application, which are defined by the appended claims and their equivalents.

Claims

1. A process for preparing microbial organic fertilizer for preventing root rot in Sichuan pepper, characterized in that, include: S1. Extract strains from the rhizosphere soil and roots of diseased plants of Sichuan pepper, screen out microorganisms with antagonistic activity against the pathogen of Sichuan pepper root rot, and compound the microorganisms into a compound strain according to a predetermined ratio; S2. Mix the fully decomposed organic substrate with the auxiliary materials in a predetermined ratio to form a solid-state fermentation substrate; S3. The compound microbial strain is inoculated into the fermentation substrate to form a pre-culture substrate, and preliminary pre-culture is carried out according to the set inoculation amount; S4. The pre-culture substrate is placed in a solid-state fermentation device equipped with online multi-parameter monitoring and intelligent control. Through the linkage adjustment of temperature, aeration rate, humidity and pH value, multi-stage refined fermentation is carried out to obtain fermented material. S5. The fermented material is dehydrated, granulated and dried to form a finished microbial organic fertilizer.

2. The preparation process of the root rot resistant microbial organic fertilizer for Sichuan pepper according to claim 1, characterized in that: The compound microbial strain is composed of Bacillus, Pseudomonas fluorescens, and Trichoderma in a mass ratio of 1:1:

1.

3. The preparation process of the root rot resistant microbial organic fertilizer for Sichuan pepper according to claim 1, characterized in that: The organic substrate is a mixture of livestock and poultry manure, organic straw, oil residue and auxiliary materials in a mass ratio of 4:3:2:1, and the moisture content of the organic substrate is 60% to 70%.

4. The preparation process of the root rot resistant microbial organic fertilizer for Sichuan pepper according to claim 1, characterized in that: The pre-culture conditions are: temperature 30℃~35℃, relative humidity 50%~60%, and time 24~48h.

5. The preparation process of the root rot resistant microbial organic fertilizer for Sichuan pepper according to claim 1, characterized in that: The solid-state fermentation device controls cell proliferation using an improved Logistic model, the model formula of which is as follows: in, Fermentation time The subsequent bacterial cell concentration, This represents the initial cell concentration at the end of the pre-culture. This represents the maximum cell concentration that can be achieved during fermentation. For the maximum specific growth rate, This refers to the fermentation time.

6. The preparation process of the root rot resistant microbial organic fertilizer for Sichuan pepper according to claim 1, characterized in that: The ventilation rate is dynamically adjusted according to the oxygen transfer model, and the model formula is as follows: Where OTR is the oxygen transfer rate. The volumetric mass transfer coefficient is . This refers to the saturated dissolved oxygen concentration in the air. This represents the actual dissolved oxygen concentration in the culture medium.

7. The preparation process of the root rot resistant microbial organic fertilizer for Sichuan pepper according to claim 1, characterized in that: The pH value range is 6.8 to 7.2, and the solid-state fermentation device maintains the pH value within the range by automatically adding buffer solution.

8. The process for preparing the root rot resistant microbial organic fertilizer for Sichuan pepper according to claim 1, characterized in that: The moisture content of the fermented material after the dehydration treatment is ≤12%, the particle size of the fermented material after the granulation treatment is 3-5mm, and the drying treatment is drying at 50℃-60℃ for 2-4h.

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