A traditional Chinese medicine composition and application thereof in preparation of an anti-fatigue health care wine

Abstract

The application provides a traditional Chinese medicine composition and application thereof in preparation of anti-fatigue health care wine, and relates to the technical fields of traditional Chinese medicine pharmacy and health care. The traditional Chinese medicine composition is prepared from medicinal and edible traditional Chinese medicinal materials, and is composed of Cistanche, Acanthopanax, Radix Asparagi, Phyllanthus emblica, Typha angustifolia, Terminalia, Chrysanthemum, Rubus corchorifolius, Canavalia, and Citrus medica, and has the effects of tonifying kidney and spleen, benefiting qi and nourishing yin, and clearing and tonifying. The traditional Chinese medicine composition and the health care wine prepared from the traditional Chinese medicine composition have excellent anti-fatigue effects, can significantly prolong the exhaustive swimming time of mice under load, significantly reduce the urea nitrogen content in plasma of mice after exercise, significantly increase the liver glycogen content of mice, significantly increase the blood lactic acid clearance rate of mice after exercise, and have a significant effect of relieving physical fatigue. The traditional Chinese medicine composition is expected to be developed into an anti-fatigue drug or health care product.

Description

Technical Field

[0001] This invention relates to the fields of traditional Chinese medicine manufacturing and health care technology, specifically to a traditional Chinese medicine composition and its application in the preparation of anti-fatigue health wine. Background Technology

[0002] With the accelerating pace of life and increasing pressure from all sides, the proportion of people in a sub-healthy state has risen sharply. Fatigue is a major manifestation of sub-health, a pre-disease state, and a pathogenic signal. Fatigue refers to the physiological phenomenon that occurs when the body cannot maintain a specific level of its function or the original intensity of an organ's activity during physiological processes. Prolonged fatigue can lead to immune dysfunction and organ damage, seriously threatening human health. For a long time, the mainstream international approach to relieving fatigue has been drug therapy. On the one hand, drug therapy is rapid and effective; on the other hand, the side effects, addictiveness, and dependence associated with conventional drug therapy greatly limit its clinical application. Therefore, the effective prevention of fatigue has become an urgent problem to be solved.

[0003] Long-term clinical research in Traditional Chinese Medicine (TCM) has shown that many traditional Chinese medicines have positive effects on relieving and treating fatigue, exhibiting advantages such as low toxicity and side effects, and have received widespread attention from scholars both domestically and internationally. Wine has a long history in my country, from its initial natural formation to the grain-based brewing techniques of early agricultural societies, and the continuous improvement of brewing technology, resulting in an increasing variety of wines. The development of Chinese wine spans thousands of years. Besides daily consumption, generations of medical scientists have highly valued the medicinal properties of wine, using it in various aspects such as processing and preparing medicinal herbs. In modern times, with the improvement of living standards and the increasing awareness of health preservation, the popularity of medicinal wines with health-preserving effects has also been rising.

[0004] Health-promoting wines are made from distilled spirits, fermented wines, or edible alcohol as a base, using traditional Chinese medicine as raw materials, and adding food additives to enhance flavor, color, and aroma, following a specific production process. Health-promoting wines are mainly used to assist in regulating bodily functions, achieving specific health benefits such as relieving fatigue, promoting blood circulation, or improving sleep quality. Currently, there are many types of anti-fatigue health-promoting wines on the market, but they suffer from various problems such as complex formulas, unclear effects, and low levels of effective active ingredients. Therefore, this invention aims to provide a traditional Chinese medicine composition with anti-fatigue effects and to use it in the preparation of a medicinal wine with anti-fatigue health-promoting functions. Summary of the Invention

[0005] (a) Technical problems to be solved

[0006] To address the shortcomings of existing technologies, this invention provides a traditional Chinese medicine composition and its application in the preparation of an anti-fatigue health wine.

[0007] (II) Technical Solution

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] In a first aspect, the present invention provides a traditional Chinese medicine composition with anti-fatigue effects, the traditional Chinese medicine composition being composed of the following raw materials in parts by weight: 10-30 parts of Cistanche deserticola, 10-30 parts of Acanthopanax senticosus, 10-30 parts of Asparagus cochinchinensis, 10-30 parts of Phyllanthus emblica, 10-30 parts of Typha orientalis, 10-30 parts of Terminalia chebula, 10-30 parts of Chicory, 10-30 parts of Brussels sprouts, 10-30 parts of Canavalia gladiata, and 10-30 parts of Citronella arvense.

[0010] Furthermore, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 15-30 parts of Cistanche deserticola, 15-30 parts of Acanthopanax senticosus, 15-30 parts of Asparagus cochinchinensis, 15-30 parts of Phyllanthus emblica, 15-30 parts of Typha orientalis, 15-30 parts of Terminalia chebula, 15-30 parts of Chicory, 15-30 parts of Brussels sprouts, 15-30 parts of Canavalia gladiata, and 15-30 parts of Citronella arvense.

[0011] Furthermore, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 28 parts of Cistanche deserticola, 24 parts of Acanthopanax senticosus, 22 parts of Asparagus cochinchinensis, 22 parts of Phyllanthus emblica, 20 parts of Typha orientalis, 20 parts of Terminalia chebula, 26 parts of Chicory, 22 parts of Brussels sprouts, 20 parts of Canavalia gladiata, and 20 parts of Citronella arvense.

[0012] The traditional Chinese medicine composition of the present invention can be used to prepare anti-fatigue drugs or health products. The drugs or health products are prepared into oral dosage forms using the above-mentioned parts by weight of medicinal materials as raw materials and supplemented with medical or health product acceptable excipients. The dosage forms include oral liquids, granules, freeze-dried powders, powders, granules, pills, etc.

[0013] Traditional Chinese medicine believes that fatigue is mostly caused by deficiency of kidney essence, insufficient qi and blood, yin deficiency and depletion of body fluids, and stagnation of qi. The kidney is the foundation of innate constitution and the organ of strength. Sufficient kidney essence results in strong bones and muscles and abundant energy. The herbal composition of this invention uses Cistanche deserticola, which specializes in tonifying kidney yang and replenishing essence and blood, as the principal herb to tonify the innate kidney and nourish primordial qi. Acanthopanax senticosus can invigorate qi and strengthen the spleen, tonify the kidney and calm the mind. Its tonification of the acquired spleen nourishes the innate kidney, which enhances the kidney-tonifying effect of Cistanche deserticola and improves the spleen deficiency symptoms such as poor appetite and weakness that accompany fatigue, thus achieving simultaneous tonification of both innate and acquired constitution. Asparagus cochinchinensis nourishes yin and moistens dryness, generates body fluid and quenches thirst. It can both nourish yin and tonify the kidney and counteract the warming and drying nature of Cistanche deserticola, achieving the effect of tonifying yang without harming yin. Acanthopanax senticosus and Asparagus cochinchinensis together serve as assistant herbs, strengthening the efficacy of the principal herb while compensating for its bias. Phyllanthus emblica and Terminalia chebula are excellent for clearing heat, cooling blood, and quenching thirst. They can help Asparagus cochinchinensis enhance its yin-nourishing and fluid-generating effects, while also treating deficiency-heat caused by weakness and clearing internal heat. Typha pollen invigorates blood and unblocks collaterals, combining tonification and purgation without causing stagnation of qi and blood. Canavalia gladiata warms the kidneys, assists yang, and relieves nausea and vomiting, helping Cistanche deserticola enhance its kidney-tonifying effects. Its milder nature also helps to moderate the warming and drying properties of Cistanche deserticola. Phyllanthus emblica, Terminalia chebula, Typha pollen, and Canavalia gladiata are all adjuvant herbs. Chicory clears the liver and gallbladder, promotes urination and reduces swelling. Bruguiera gymnorhiza leaf promotes digestion, clears heat and eliminates dampness. Long-term use of tonifying herbs such as Cistanche deserticola and Acanthopanax senticosus can easily cause greasiness, indigestion, and dampness and heat. These two herbs can clear damp-heat, promote digestion and harmonize the stomach, preventing the body from being unable to tolerate tonification, and are therefore adjuvant herbs. Citron, with its aromatic and dispersing properties, can guide the effects of other herbs to flow smoothly throughout the body, ensuring that the entire formula is nourishing without causing stagnation or cloying stagnation. It also harmonizes the other herbs, acting as a guiding herb. Together, these herbs form a traditional Chinese medicine composition that tonifies the kidneys and spleen, replenishes qi and nourishes yin, and provides both clearing and tonifying effects, making it suitable for combating fatigue.

[0014] Secondly, this invention provides the application of the above-mentioned traditional Chinese medicine composition in the preparation of an anti-fatigue health wine, wherein the anti-fatigue health wine is prepared according to the following steps:

[0015] (1) Weigh out the following Chinese medicinal materials according to the following weight proportions: 10-30 parts of Cistanche deserticola, 10-30 parts of Acanthopanax senticosus, 10-30 parts of Asparagus cochinchinensis, 10-30 parts of Phyllanthus emblica, 10-30 parts of Typha orientalis, 10-30 parts of Terminalia chebula, 10-30 parts of Chicory, 10-30 parts of Brussels sprouts, 10-30 parts of Canavalia gladiata, and 10-30 parts of Citronella foenum-graecum; wash, dry, crush or slice, and then mix evenly.

[0016] (2) Take the Chinese herbal raw materials mentioned in step (1), then add 3-6 times the weight of the Chinese herbal raw materials and mix evenly to obtain Chinese herbal wine starter;

[0017] (3) Soak the washed grain raw materials in clean water. The water level should completely submerge the grain and be at least 12 cm above it. Soak for 12-24 hours. Then take out the grain, drain the water, and steam it in a brewing steamer. Then cool the steamed grain to below 35°C for later use.

[0018] (4) Mix the Chinese herbal wine starter prepared in step (1) and the steamed grain evenly, and carry out saccharification at 25-28℃ for 20-24 hours. After saccharification, add 0.5-0.8% of the grain raw material's starter and mix evenly. Then transfer it to a fermentation container and ferment at 25-28℃ for more than 25 days.

[0019] (5) After fermentation, pour the fermented mash into a distillation apparatus for distillation, collect the distilled liquid, discard the first liquor, and take the middle liquor as the base liquor for later use.

[0020] (6) Take 10-20 parts by weight of jujube, 10-20 parts of licorice and 10-20 parts of kudzu root, wash, dry and slice them, and mix them evenly with the Chinese herbal raw materials in step (1). Put them in a container, pour in the base liquor, and the base liquor must completely submerge the Chinese herbal raw materials and the liquid level must be at least 15 cm above the raw materials. Soak for more than 15 days. During the soaking period, it is necessary to stir regularly so that the Chinese herbal raw materials and the base liquor can be in full contact.

[0021] (7) After soaking, filter to separate the residue and wine. Let the filtered wine stand to clarify. Take the clarified wine, seal and store it, and age it in a cool, dark place at 20°C for 1-6 months. After aging, the anti-fatigue health wine is obtained.

[0022] Furthermore, in step (3), the grain raw materials are one or more of sorghum, rice, wheat, corn, and glutinous rice.

[0023] Furthermore, in step (4), the amount of Chinese medicinal wine yeast added accounts for 0.3-0.6% of the weight of the grain raw materials;

[0024] Furthermore, in step (6), the role of jujube, licorice, and kudzu root is to flavor, sweeten, and enhance the aroma, making the wine smoother.

[0025] (III) Beneficial Effects

[0026] This invention relates to a traditional Chinese medicine composition using medicinal and edible herbs as raw materials, comprising Cistanche deserticola, Acanthopanax senticosus, Asparagus cochinchinensis, Phyllanthus emblica, Typha orientalis, Terminalia chebula, Chicory, Brussels sprouts, Canavalia gladiata, and Citronella arvense. It possesses the effects of tonifying the kidneys and spleen, replenishing qi and nourishing yin, and providing both clearing and tonifying benefits. The traditional Chinese medicine composition provided by this invention, and the health-preserving wine prepared using this composition, exhibit excellent anti-fatigue effects. It can significantly prolong the time of exhausted swimming under load in mice, significantly reduce the plasma urea nitrogen content in mice after exercise, significantly increase the liver glycogen content in mice, and significantly increase the blood lactate clearance rate in mice after exercise, thus significantly relieving physical fatigue. It holds promise for development into anti-fatigue drugs or health products. Attached Figure Description

[0027] Figure 1 The change in body weight of mice in each group during the experiment.

[0028] Figure 2 For the anti-fatigue effect results of the traditional Chinese medicine composition of the present invention, A is the time to exhaustion during swimming; B is the plasma urea nitrogen content; C is the liver glycogen content; D is the blood lactate clearance rate; wherein, the same lowercase letter indicates P>0.05, and different lowercase letters indicate P<0.05.

[0029] Figure 3 For the anti-fatigue effect results of the health wine of the present invention, A is the time to exhaustion during swimming; B is the plasma urea nitrogen content; C is the liver glycogen content; D is the blood lactate clearance rate; wherein, the same lowercase letter indicates P>0.05, and different lowercase letters indicate P<0.05. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0031] Example 1

[0032] A traditional Chinese medicine composition with anti-fatigue effect, the composition comprising the following raw materials in parts by weight: 28 parts of Cistanche deserticola, 24 parts of Acanthopanax senticosus, 22 parts of Asparagus cochinchinensis, 22 parts of Phyllanthus emblica, 20 parts of Typha orientalis, 20 parts of Terminalia chebula, 26 parts of Chicory, 22 parts of Brussels sprouts, 20 parts of Canavalia gladiata, and 20 parts of Citronella arvense.

[0033] The above-mentioned traditional Chinese medicine composition can be used to prepare an anti-fatigue health wine, which is prepared according to the following steps:

[0034] (1) Weigh the following Chinese medicinal materials according to the following weight proportions: 28 parts of Cistanche deserticola, 24 parts of Acanthopanax senticosus, 22 parts of Asparagus cochinchinensis, 22 parts of Phyllanthus emblica, 20 parts of Typha orientalis, 20 parts of Terminalia chebula, 26 parts of Chicory, 22 parts of Brussels sprouts, 20 parts of Canavalia gladiata, and 20 parts of Citronella arvense; wash, dry, crush or slice, and then mix evenly;

[0035] (2) Take the Chinese herbal raw materials mentioned in step (1), then add small yeast that accounts for 5 times the weight of the Chinese herbal raw materials, mix evenly and you will get Chinese herbal wine yeast;

[0036] (3) Soak the washed grain raw materials in clean water. The water level should completely submerge the grain and be at least 12 cm above it. Soak for 12-24 hours. Then take out the grain, drain the water, and steam it in a brewing steamer. Then cool the steamed grain to below 35°C and set aside. The grain raw materials can be one or more of sorghum, rice, wheat, corn, and glutinous rice.

[0037] (4) Mix the Chinese herbal wine starter prepared in step (1) with the steamed grains evenly, and carry out saccharification at 25-28℃ for 20-24 hours. After saccharification, add 0.7% of the grain raw material weight of the starter and mix evenly. Then transfer it to a fermentation container and ferment at 25-28℃ for more than 25 days. The amount of Chinese herbal wine starter added accounts for 0.5% of the grain raw material weight.

[0038] (5) After fermentation, pour the fermented mash into a distillation apparatus for distillation, collect the distilled liquid, discard the first liquor, and take the middle liquor as the base liquor for later use.

[0039] (6) Take another 20 parts by weight of jujube, 20 parts of licorice and 20 parts of kudzu root, wash, dry and slice them and mix them evenly with the Chinese herbal raw materials in step (1), put them in a container, pour in the base wine, the base wine must completely submerge the Chinese herbal raw materials and the liquid level must be at least 15 cm above the base wine; soak for more than 15 days, and stir regularly during the soaking period to ensure that the Chinese herbal raw materials are in full contact with the base wine;

[0040] (7) After soaking, filter to separate the residue and wine. Let the filtered wine stand to clarify. Take the clarified wine, seal and store it, and age it in a cool, dark place at 20°C for 1-6 months. After aging, the anti-fatigue health wine is obtained.

[0041] Example 2

[0042] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 14 parts of Cistanche deserticola, 14 parts of Acanthopanax senticosus, 12 parts of Asparagus cochinchinensis, 12 parts of Phyllanthus emblica, 12 parts of Typha pollen, 12 parts of Terminalia chebula, 12 parts of Chicory, 12 parts of Brussels sprouts, 12 parts of Canavalia gladiata, and 12 parts of Citronella arvense.

[0043] Example 3

[0044] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of Cistanche deserticola, 30 parts of Acanthopanax senticosus, 30 parts of Asparagus cochinchinensis, 30 parts of Phyllanthus emblica, 30 parts of Typha pollen, 30 parts of Terminalia chebula, 30 parts of Chicory, 30 parts of Brussels sprouts, 30 parts of Canavalia gladiata, and 30 parts of Citronella arvense.

[0045] Example 4

[0046] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 28 parts of Cistanche deserticola, 26 parts of Acanthopanax senticosus, 26 parts of Asparagus cochinchinensis, 24 parts of Phyllanthus emblica, 22 parts of Typha orientalis, 24 parts of Terminalia chebula, 28 parts of Chicory, 26 parts of Brussels sprouts, 26 parts of Canavalia gladiata, and 22 parts of Citronella arvense.

[0047] Example 5

[0048] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of Cistanche deserticola, 18 parts of Acanthopanax senticosus, 16 parts of Asparagus cochinchinensis, 18 parts of Phyllanthus emblica, 16 parts of Typha pollen, 16 parts of Terminalia chebula, 18 parts of Chicory, 20 parts of Brussels sprouts, 16 parts of Canavalia gladiata, and 16 parts of Citronella arvense.

[0049] Example 6

[0050] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 28 parts of Cistanche deserticola, 28 parts of Acanthopanax senticosus, 26 parts of Asparagus cochinchinensis, 20 parts of Phyllanthus emblica, 22 parts of Typha orientalis, 26 parts of Terminalia chebula, 24 parts of Chicory, 22 parts of Brussels sprouts, 22 parts of Canavalia gladiata, and 20 parts of Citronella arvense.

[0051] Example 7

[0052] The difference between this embodiment and Embodiment 1 is that the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 18 parts of Cistanche deserticola, 18 parts of Acanthopanax senticosus, 15 parts of Asparagus cochinchinensis, 15 parts of Phyllanthus emblica, 15 parts of Typha pollen, 15 parts of Terminalia chebula, 16 parts of Chicory, 15 parts of Brussels sprouts, 16 parts of Canavalia gladiata, and 16 parts of Citronella arvense.

[0053] Comparative Example 1

[0054] A grain-based liquor is prepared according to the following steps:

[0055] (1) Soak the washed grain raw materials in clean water. The water level should completely submerge the grain and be at least 12 cm above it. Soak for 12-24 hours. Then take out the grain, drain the water, and steam it in a brewing steamer. Then cool the steamed grain to below 35°C and set aside. The grain raw materials can be one or more of sorghum, rice, wheat, corn, and glutinous rice.

[0056] (2) Mix 0.5% of the weight of the grain raw material with the steamed grain evenly, and carry out saccharification at 25-28℃ for 20-24 hours. After saccharification, add 0.7% of the weight of the grain raw material and mix evenly. Then transfer to a fermentation container and ferment at 25-28℃ for more than 25 days.

[0057] (3) After fermentation, pour the fermented mash into a distillation apparatus for distillation, collect the distilled liquid, discard the first liquor, and take the middle liquor as the base liquor for later use.

[0058] (4) Take another 20 parts by weight of jujube, 20 parts of licorice and 20 parts of kudzu root, wash, dry and slice them, mix them together, put them in a container, pour in the base wine, the base wine must be completely submerged and the liquid level must be at least 15 cm above the base wine; soak for more than 15 days, and stir regularly during the soaking period to ensure that the jujube, licorice and kudzu root are in full contact with the base wine.

[0059] (5) After soaking, filter to separate the residue and the wine. Let the filtered wine stand to clarify. Take the clarified wine, seal and store it, and age it in a cool, dark place at 20°C for 1-6 months. After aging, the wine made from the grain is obtained.

[0060] Experimental Example 1

[0061] Safety evaluation of the anti-fatigue health wine prepared by this invention

[0062] 1. Experimental Methods

[0063] Forty healthy SPF-grade Kunming mice, weighing 18-20g, with equal numbers of males and females, were randomly divided into a control group and a health wine group (n=20 each) after 7 days of acclimatization. The health wine group (using a 20-fold concentrated solution of the health wine prepared in Example 1) was administered via gavage at a dose of 200mg / kg / mouse per day, at a volume of 0.02mL / g. The control group was administered an equal volume of physiological saline via gavage. Both groups were administered the solution via gavage for 14 consecutive days, maintaining identical feeding conditions. During the experiment, the activity and behavioral changes of the mice in each group were observed. The body weight of the mice was measured before and after the experiment. After the experiment, the livers and kidneys of the mice were weighed, and organ indices were calculated.

[0064] Organ Index = Organ Mass (g) / Mouse Body Mass (g) × 100%

[0065] 2. Experimental Results

[0066] 2.1 General Behavioral Observation

[0067] Within 14 days of gavage administration, no mice in either group died. No abnormalities or severe mortality were observed in the mice before or after administration, therefore the LD50 could not be determined. During the experiment, the mice in all groups grew well, and their body weight increased with prolonged feeding time. No abnormalities were observed in their diet, behavior, or appearance. The results are shown in Table 1.

[0068] 2.2 Organ Index

[0069] Compared with the control group, there were no significant differences in liver and kidney indices in the health wine group (P>0.05). The results are shown in Table 1.

[0070] Table 1. Results of body weight, liver index, and kidney index of mice in each group.

[0071]

[0072] Note: *P<0.05 compared to the control group.

[0073] The above results indicate that the health tonic prepared from the traditional Chinese medicine composition provided by this invention has no toxic side effects on the body.

[0074] Experimental Example 2

[0075] The present invention provides a traditional Chinese medicine combination and explores the anti-fatigue effect of the health wine prepared using the traditional Chinese medicine combination.

[0076] 1. Materials and Methods

[0077] 1.1 Laboratory Animals

[0078] Adult male SPF-grade Kunming mice, weighing 18-22g, were acclimatized for 7 days before the experiment.

[0079] 1.2 Experimental Drugs

[0080] The herbal composition extract of this invention consists of: 28 parts of Cistanche deserticola, 24 parts of Acanthopanax senticosus, 22 parts of Asparagus cochinchinensis, 22 parts of Phyllanthus emblica, 20 parts of Typha orientalis, 20 parts of Terminalia chebula, 26 parts of Chicory, 22 parts of Brussels sprouts, 20 parts of Canavalia gladiata, and 20 parts of Citronella arvense.

[0081] The control group of Chinese herbal extracts consisted of: 28 parts of Cistanche deserticola, 22 parts of Asparagus cochinchinensis, 22 parts of Phyllanthus emblica, 20 parts of Typha orientalis, 20 parts of Terminalia chebula, 20 parts of Canavalia gladiata, and 20 parts of Citronella foenum-graecum.

[0082] The control group of Chinese herbal extracts consisted of: 28 parts of Cistanche deserticola, 24 parts of Acanthopanax senticosus, 22 parts of Asparagus cochinchinensis, 26 parts of Chicory, 22 parts of Brussels sprouts, and 20 parts of Citronella arvense.

[0083] The control group of Chinese herbal extracts consisted of: 28 parts of Cistanche deserticola, 22 parts of Phyllanthus emblica, 20 parts of Typha pollen, 20 parts of Terminalia chebula, 26 parts of Chicory, 22 parts of Brussels sprouts, and 20 parts of Canavalia gladiata.

[0084] The above-mentioned Chinese herbal medicine composition extract is prepared according to the following steps: weigh each raw material according to the above-mentioned weight proportions, wash them, and extract them by reflux with 65% ethanol. Repeat the reflux extraction 3 times, collect the ethanol extracts 3 times, filter, evaporate the solvent until the ethanol content is below 5%, and then prepare a 0.5g / mL extract with purified water for later use.

[0085] The anti-fatigue health wine prepared in Example 1.

[0086] The wine prepared in Comparative Example 1.

[0087] 1.3 Grouping and Processing

[0088] Three hundred and twenty male Kunming mice were randomly divided into four groups of eighty each, and subjected to weight-bearing swimming tests, plasma urea assays, liver glycogen assays, and blood lactate assays. Each group of eighty male mice was further divided into eight subgroups of ten mice each. Specific grouping and treatment details are shown in Table 2. All mice were administered the drug for 30 consecutive days, and an anti-fatigue experiment was immediately conducted after 30 days.

[0089] Table 2 Grouping and Processing Methods

[0090]

[0091] 1.4 Mouse Swimming Experiment Under Weight-Bearing Exhaustion

[0092] One hour before the experiment, mice were fasted but allowed free access to water. Thirty minutes after the last gavage, a weight equivalent to 5% of the mouse's body weight was tied to the base of each mouse's tail. The mice were then placed in a swimming tank at a depth of over 30 cm and a water temperature of (25±1.0)℃ for the weighted swimming test. The time from the start of swimming to complete exhaustion and submersion, and the inability to surface for breath within 10 seconds, was recorded. During the experiment, the water in the tank was gently stirred with a stick to keep it flowing. Once the mice reached exhaustion, they were quickly removed from the water and their fur was dried with a hairdryer.

[0093] 1.5 Blood Urea Nitrogen (BUN) Measurement

[0094] Thirty minutes after the last gavage administration of the sample to the mice, the mice were placed in a swimming tank for swimming at a water temperature of (30±1)℃ for 90 minutes. After swimming, the mice were removed and allowed to rest for 60 minutes before blood was collected from their eyeballs. After centrifugation of the collected blood, 20 μL of plasma was aspirated and the BUN value was measured using a serum urea nitrogen (BUN) test kit.

[0095] 1.6 Liver glycogen assay

[0096] Thirty minutes after the last gavage, the mice were immediately sacrificed, and the livers were removed, rinsed with physiological saline, blotted dry with filter paper, and 75 mg of liver was accurately weighed. The liver glycogen content was determined using a liver glycogen assay kit.

[0097] 1.7 Blood lactate (BLA) measurement

[0098] Thirty minutes after the last gavage administration of the sample to the mice, the mice were placed in a swimming tank for swimming. The water temperature was controlled at (30±1)℃, and the swimming time was 10 minutes. Blood samples were collected from the inner canthal venous plexus of the mice at 0 minutes and 20 minutes after swimming, and the BLA content of the mice was measured using a lactate assay kit. The BLA clearance rate was calculated.

[0099] BLA clearance rate [mmol / (L·min)] = (BLA content at 0 min after swimming - BLA content at 20 min after swimming) / 20 min

[0100] 1.8 Statistics and Analysis

[0101] SPSS 19.0 software was used to analyze the raw data. The data of each group are expressed as mean ± standard deviation. One-way ANOVA was used for intergroup analysis. If the variances were unequal, Tamhane's T2 (multiple comparisons based on t test) was used for analysis. P < 0.05 was considered statistically significant.

[0102] 2 Results

[0103] 2.1 Mouse body weight

[0104] like Figure 1 As shown, after 30 days of intervention, there was no significant difference in body weight change among the groups of mice (P>0.05). Throughout the experiment, the mice in each group exhibited normal daily activities and good mental state, and no deaths or poisoning occurred, indicating that the traditional Chinese medicine composition and health wine provided by this invention have no significant effect on the growth of mice.

[0105] 2.2 Anti-fatigue effect of the traditional Chinese medicine composition of the present invention

[0106] Figure 2 A represents the exhaustive swimming time of mice in the control group and the groups treated with different formulations of traditional Chinese medicine extracts. Compared with the control group (NC), the exhaustive swimming time of mice in each treatment group was significantly increased (P<0.05); among them, the exhaustive swimming time of mice in the traditional Chinese medicine group (TC) of this invention was significantly higher than that of the control traditional Chinese medicine group 1-3 (Com-1-3), with a significant difference (P<0.05).

[0107] Figure 2 B represents the plasma urea nitrogen content of mice in the control group and the groups treated with different formulations of traditional Chinese medicine extracts 60 minutes after exercise. Compared with the NC group, the plasma urea nitrogen content of mice in each treatment group was significantly lower (P<0.05); among them, the plasma urea nitrogen content of mice in the TC group was significantly lower than that of Com-1 and Com-3 (P<0.05), but lower than that of Com-2 (P>0.05).

[0108] Figure 2 C represents the liver glycogen content of mice in the control group and the groups treated with different formulations of traditional Chinese medicine extracts after administration. Compared with the NC group, the liver glycogen content of mice in each administration group was significantly increased (P<0.05); among them, the exhaustive swimming time of mice in the TC group was significantly higher than that in the Com-1-3 group (P<0.05).

[0109] Figure 2D represents the blood lactate (BLA) clearance rate after exercise in mice in the control group and the groups treated with different formulations of traditional Chinese medicine extracts. Compared with the NC group, the BLA clearance rate of mice in each treatment group was significantly increased (P<0.05); among them, the BLA clearance rate of mice in the TC group was significantly higher than that in Com-1 and Com-3 (P<0.05), and higher than that in Com-2 (P>0.05).

[0110] 2.3 The anti-fatigue effect of the health tonic of this invention

[0111] Figure 3 A represents the exhaustive swimming time of mice in the control group, high- and low-dose health wine groups, and control wine group. Compared with the control group (NC), the exhaustive swimming time of mice in the high- and low-dose health wine groups (BJJ-H and BJJ-L) was significantly increased (P<0.05), while the exhaustive swimming time of mice in the control wine group (DZJ) was slightly increased but not significantly different (P>0.05).

[0112] Figure 3 B represents the plasma urea nitrogen content of mice in the control group, high- and low-dose health wine groups, and control wine group 60 minutes after exercise. Compared with the NC group, the plasma urea nitrogen content of mice in the BJJ-H and BJJ-L groups was significantly lower (P<0.05); the plasma urea nitrogen content of mice in the DZJ group was not significantly different from that in the NC group (P>0.05).

[0113] Figure 3 C represents the liver glycogen content results after the administration of the control group, the high-dose and low-dose health wine groups, and the control wine group. Compared with the NC group, the liver glycogen content of mice in the BJJ-H and BJJ-L groups was significantly increased (P<0.05); the liver glycogen content of mice in the DZJ group was not significantly different from that in the NC group (P>0.05).

[0114] Figure 3 D represents the blood lactate (BLA) clearance rate after exercise in mice from the control group, high- and low-dose health wine groups, and control wine group. Compared with the NC group, the BLA clearance rate of mice in the BJJ-H and BJJ-L groups was significantly increased (P<0.05); the BLA clearance rate of mice in the DZJ group was not significantly different from that in the NC group (P>0.05).

[0115] 3. Conclusion

[0116] Physical fatigue, also known as exercise-induced fatigue, is a physiological protective mechanism of the body against excessive physical activity. When fatigue occurs, not only does the body's work and exercise capacity decline, but the body also exhibits a sub-healthy state or early signs of disease. Furthermore, if physical fatigue persists for a long period, the risk of premature aging and organic diseases increases significantly. The exhaustive swimming test, plasma urea nitrogen, liver glycogen, and blood lactate are important indicators for detecting physical fatigue.

[0117] Decreased athletic performance is the most direct external manifestation of physical fatigue. Weighted swimming experiments can reflect the degree of decline in athletic performance and are also an important indicator of the ability to alleviate physical fatigue. The body's main energy source is blood glucose. After prolonged strenuous exercise, a large amount of energy is consumed, and blood glucose levels drop. When energy supply is insufficient, the body breaks down protein to ensure functional needs. Protein breakdown produces ammonia, which is metabolized into urea. The plasma urea nitrogen content increases with the animal's workload; the stronger the body's adaptability to exercise, the lower the plasma urea nitrogen content. The ability of a sample to inhibit or clear plasma urea nitrogen is positively correlated with its anti-fatigue effect. Glycogen is an important energy source for the body, and liver glycogen is one of the main ways to store glycogen. Glycogen is broken down into glucose to meet the needs of exercise. Glycogen storage is closely related to exercise endurance; excessive glycogen consumption can cause fatigue. Lactic acid is produced during strenuous exercise when muscles undergo glycolysis due to hypoxia. A large accumulation of lactic acid leads to muscle soreness and fatigue. Therefore, the stronger the ability to clear blood lactate, the more significant the anti-fatigue effect.

[0118] The experimental results show that the traditional Chinese medicine combination provided by this invention can significantly prolong the time of mice swimming to exhaustion under load, significantly reduce the plasma urea nitrogen content in mice after exercise, significantly increase the liver glycogen content in mice, and significantly increase the blood lactate clearance rate in mice after exercise. It has a significant effect in relieving physical fatigue, and its fatigue-relieving effect is significantly better than that of traditional Chinese medicine compositions with other formulations. Meanwhile, the health-preserving wine prepared using the traditional Chinese medicine combination of this invention can also significantly prolong the time of mice swimming to exhaustion under load, significantly reduce the plasma urea nitrogen content in mice after exercise, significantly increase the liver glycogen content in mice, and significantly increase the blood lactate clearance rate in mice after exercise, exhibiting excellent anti-fatigue health-preserving effects.

[0119] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A traditional Chinese medicine composition with anti-fatigue effect, characterized in that, The traditional Chinese medicine composition consists of the following raw materials in parts by weight: 10-30 parts of Cistanche deserticola, 10-30 parts of Acanthopanax senticosus, 10-30 parts of Asparagus cochinchinensis, 10-30 parts of Phyllanthus emblica, 10-30 parts of Typha orientalis, 10-30 parts of Terminalia chebula, 10-30 parts of Chicory, 10-30 parts of Brussels sprouts, 10-30 parts of Canavalia gladiata, and 10-30 parts of Citronella arvense.

2. The traditional Chinese medicine composition with anti-fatigue effect according to claim 1, characterized in that, The traditional Chinese medicine composition consists of the following raw materials in parts by weight: 15-30 parts of Cistanche deserticola, 15-30 parts of Acanthopanax senticosus, 15-30 parts of Asparagus cochinchinensis, 15-30 parts of Phyllanthus emblica, 15-30 parts of Typha orientalis, 15-30 parts of Terminalia chebula, 15-30 parts of Chicory, 15-30 parts of Brussels sprouts, 15-30 parts of Canavalia gladiata, and 15-30 parts of Citronella arvense.

3. The traditional Chinese medicine composition with anti-fatigue effect according to claim 1, characterized in that, The traditional Chinese medicine composition consists of the following raw materials in parts by weight: 28 parts of Cistanche deserticola, 24 parts of Acanthopanax senticosus, 22 parts of Asparagus cochinchinensis, 22 parts of Phyllanthus emblica, 20 parts of Typha pollen, 20 parts of Terminalia chebula, 26 parts of Chicory, 22 parts of Brussels sprouts, 20 parts of Canavalia gladiata, and 20 parts of Citronella arvense.

4. The application of the traditional Chinese medicine composition according to any one of claims 1 to 3 in the preparation of an anti-fatigue health wine, characterized in that, The anti-fatigue health wine is prepared according to the following steps: (1) Weigh out the following Chinese medicinal materials according to the following weight proportions: 10-30 parts of Cistanche deserticola, 10-30 parts of Acanthopanax senticosus, 10-30 parts of Asparagus cochinchinensis, 10-30 parts of Phyllanthus emblica, 10-30 parts of Typha orientalis, 10-30 parts of Terminalia chebula, 10-30 parts of Chicory, 10-30 parts of Brussels sprouts, 10-30 parts of Canavalia gladiata, and 10-30 parts of Citronella foenum-graecum; wash, dry, crush or slice, and then mix evenly. (2) Take the Chinese herbal raw materials mentioned in step (1), then add 3-6 times the weight of the Chinese herbal raw materials and mix evenly to obtain Chinese herbal wine starter; (3) Soak the washed grain raw materials in clean water. The water level should completely submerge the grain and be at least 12 cm above it. Soak for 12-24 hours. Then take out the grain, drain the water, and steam it in a brewing steamer. Then cool the steamed grain to below 35°C for later use. (4) Mix the Chinese herbal wine starter prepared in step (2) and the steamed grain evenly, and carry out saccharification at 25-28℃ for 20-24 hours. After saccharification, add 0.5-0.8% of the starter by weight of the grain raw material and mix evenly. Then transfer it to a fermentation container and ferment at 25-28℃ for more than 25 days. (5) After fermentation, pour the fermented mash into a distillation apparatus for distillation, collect the distilled liquid, discard the first liquor, and take the middle liquor as the base liquor for later use. (6) Take 10-20 parts by weight of jujube, 10-20 parts of licorice and 10-20 parts of kudzu root, wash, dry and slice them, and mix them evenly with the Chinese herbal raw materials in step (1). Put them in a container, pour in the base liquor, and the base liquor must completely submerge the Chinese herbal raw materials and the liquid level must be at least 15 cm above the raw materials. Soak for more than 15 days. During the soaking period, it is necessary to stir regularly so that the Chinese herbal raw materials and the base liquor can be in full contact. (7) After soaking, filter to separate the residue and wine. Let the filtered wine stand to clarify. Take the clarified wine, seal and store it, and age it in a cool, dark place at 20°C for 1-6 months. After aging, the anti-fatigue health wine is obtained.

5. The application of the traditional Chinese medicine composition according to claim 4 in the preparation of an anti-fatigue health wine, characterized in that, In step (3), the grain raw materials are one or more of sorghum, rice, wheat, corn, and glutinous rice.

6. The application of the traditional Chinese medicine composition according to claim 4 in the preparation of an anti-fatigue health wine, characterized in that, In step (4), the amount of Chinese medicinal wine yeast added accounts for 0.3-0.6% of the weight of the grain raw materials.

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