Streptomyces LGWZYY-008 and application thereof
By using Streptomyces LGWZYY-008, the lack of root immune-enhancing bacteria in tobacco was solved, enabling disease control and growth promotion in tobacco and other crops, improving tobacco yield and quality, and enhancing plant disease resistance.
Patent Information
- Application Number
- CN202511633682.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-10
- Publication Date
- 2026-01-16
AI Technical Summary
The lack of effective tobacco root immune-enhancing bacteria in existing technologies leads to a decline in tobacco field soil quality, an increase in pathogenic microorganisms, stunted tobacco plant growth, poor stress resistance, and affects tobacco leaf yield, quality, and green sustainable production.
Streptomyces sp. (LGWZYY-008) was used to promote tobacco growth and improve disease resistance. It has high reproductive capacity, diverse metabolites, and antagonistic effects against pathogens. It can be applied to the prevention and control of diseases in tobacco, pepper, tomato, Solomon's seal, eggplant, cucumber, water chestnut, strawberry, and corn.
It significantly improves tobacco yield and quality, enhances tobacco root vitality, reduces malondialdehyde content in roots, increases the control efficacy against tobacco black shank disease by up to 75%, and also has a high antagonistic effect on other crop diseases, thus enhancing the plant's disease resistance.
Smart Images
Figure CN121343833A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the field of biocontrol technology, and more particularly to a Streptomyces LGWZYY-008 and application thereof. BACKGROUND
[0002] Tobacco is an important economic crop in China, and the yield and quality of tobacco leaves are closely related to the income of tobacco farmers. Due to continuous monoculture and unreasonable fertilization by farmers for a long time, the soil quality of tobacco fields has significantly decreased, and the number of pathogenic microorganisms has increased, resulting in frequent occurrence of soil-borne diseases in tobacco-growing soil, hindered growth and development of tobacco plants, and poor overall stress resistance of tobacco plants, which seriously threatens the improvement of tobacco yield and quality and sustainable production of green tobacco.
[0003] In order to solve a series of problems caused by long-term monoculture and excessive use of fertilizers in agricultural production, plant growth promoting rhizobacteria (PGPR) has attracted much attention in recent years. Plant growth promoting rhizobacteria can improve soil nutrient content by nitrogen fixation, phosphorus dissolution and potassium dissolution, and convert insoluble substances in the soil into easily soluble substances for direct absorption and utilization by plants, thereby promoting plant growth and development. Tobacco root immune enhancer, as a kind of plant growth promoting rhizobacteria, is essentially to improve the disease resistance of tobacco and increase the yield and quality of tobacco by enhancing the activity, configuration and immunity of tobacco roots. However, there are few reports on tobacco root immune enhancer in the prior art.
[0004] In summary, how to provide a tobacco root immune enhancer and its application is a problem that needs to be solved by those skilled in the art. SUMMARY
[0005] Therefore, the present application provides a Streptomyces LGWZYY-008 and application thereof.
[0006] The biocontrol bacteria screened by the present application have the characteristics of high reproductive capacity, complex metabolic activity and multiple products, diverse modes of action on pathogenic bacteria, short life cycle, and easy artificial cultivation, and can play an important role in biological control, effectively promoting the growth and disease resistance of tobacco.
[0007] In order to achieve the above-mentioned purpose, the present application adopts the following technical solutions:
[0008] A Streptomyces LGWZYY-008, which is classified as Streptomyces sp., was preserved in China Center for Type Culture Collection on September 3, 2025, with the preservation number CCTCC NO: M20251957 and the preservation address being China. Wuhan. Wuhan University.
[0009] The application of the above-mentioned Streptomyces LGWZYY-008 in preventing and treating plant diseases.
[0010] Further, the plant is tobacco, pepper, tomato, polygonatum, eggplant, cucumber, wild rice shoot, strawberry and corn.
[0011] Further, the plant disease is cucumber sharp knife bunt, tobacco sharp cell disease, tobacco target spot disease, tobacco black foot disease, polygonatum anthracnose, strawberry blight, pepper wilt, pepper anthracnose, wild rice shoot bunt, corn blight, tomato leaf spot, tomato early blight and eggplant wilt.
[0012] The application of the above-mentioned Streptomyces LGWZYY-008 in improving the resistance of tobacco to tobacco black foot disease.
[0013] The application of the above-mentioned Streptomyces LGWZYY-008 in promoting the growth of tobacco.
[0014] Further, the promotion of the growth of tobacco is to improve the plant height, stem diameter, leaf length, leaf width, SPAD and biomass of tobacco.
[0015] The application of the above-mentioned Streptomyces LGWZYY-008 in improving the root activity and peroxidase content of tobacco.
[0016] The application of the above-mentioned Streptomyces LGWZYY-008 in reducing the malondialdehyde content of tobacco roots.
[0017] A tobacco root immune strengthening bacterial agent, comprising the above-mentioned Streptomyces LGWZYY-008.
[0018] Through the above-mentioned technical solutions, compared with the prior art, the present application has the following beneficial effects:
[0019] The Streptomyces LGWZYY-008 of the present application has immune strengthening and disease resistance promoting effects on tobacco. We verified the disease resistance promoting effects of Streptomyces LGWZYY-008 under different nitrogen fertilizer application amounts, and found that it can significantly improve the yield and quality of tobacco under 0 and 7 Kg / acre nitrogen fertilizer treatment, and the disease control effect on tobacco black foot disease reaches 75%. It has high antagonistic effect on the disease pathogens of pepper, tomato, eggplant, cucumber, wild rice shoot, strawberry and corn and the like. At the same time, it can enhance the root activity and peroxidase activity of tobacco, and reduce the malondialdehyde activity of roots. In summary, the application of Streptomyces LGWZYY-008 can provide high-quality biocontrol strains for immune strengthening of tobacco roots and biological control of diseases, and lay a foundation for yield increase and quality improvement of tobacco and green production. BRIEF DESCRIPTION OF DRAWINGS
[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings described below are only a part of the embodiments of the present application, and for those skilled in the art, other drawings can be obtained without creative labor on the basis of the provided drawings.
[0021] Figure 1 Figure of the colony morphology of the strain LGWZYY-008 in Embodiment 2 of the present application. DETAILED DESCRIPTION
[0022] The technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.
[0023] The required reagents in the present application are conventional experimental reagents, which are purchased from the market; and the experimental methods not mentioned are conventional experimental methods, which will not be described one by one here.
[0024] Embodiment 1
[0025] Isolation and screening of strains
[0026] 1. Sample collection
[0027] Rhizosphere soil of high-yield and disease-resistant healthy tobacco plants
[0028] Tobacco variety: Yunyan 87
[0029] 2. Screening of antagonistic pathogenic bacteria
[0030] The tested and screened antagonistic pathogenic bacteria is tobacco Phytophthora.
[0031] 3. Preparation of culture medium
[0032] Phytophthora culture medium: potato dextrose agar (PDA), 200 g of potato, 20 g of glucose, 18-20 g of agar powder, 1 L of distilled water, heating and dissolving, sterilization treatment. Add 1 mL of 0.1 g / mL chloramphenicol solution (sterilized water) to 1 L of culture medium, which can inhibit the growth of bacteria.
[0033] Bacterial culture medium: NB medium, 10 g / L of proteose peptone, 5 g / L of sodium chloride, 3 g / L of beef extract, 18-20 g / L of agar powder, sterilization treatment.
[0034] Sterilization: After the agar powder is completely dissolved, the non-solidified medium is divided into conical flasks and sterilized at 121°C for 20 min. Then, the medium is divided into culture dishes in a sterile environment and placed at 37°C for 24 h after solidification. The medium without growth of miscellaneous bacteria is preserved and used for the next test.
[0035] 4. Preparation of soil suspension
[0036] After 3 parts of soil samples are mixed uniformly, 10 g of soil is randomly weighed from the mixture into a 250 mL conical flask, 90 mL of sterile water is added, and the mixture is stirred on a magnetic stirrer at 190 r / min for 1 h to prepare a soil suspension.
[0037] 5. Isolation of antagonistic bacteria by dilution plate coating method
[0038] The soil suspension is diluted to 10 -4 , 10 -5 , 10 -6 times the original concentration, and 100 μL is coated on the NB medium. Each concentration is repeated three times. The mixture is placed at 30°C for 2 d, and single colonies are selected according to the colony morphology and purified by streaking. The colonies are numbered and stored at 14°C.
[0039] 6. Plate confrontation test of antagonistic bacteria
[0040] Phytophthora nicotianae is used as the target, and the plate confrontation method is used to screen antagonistic bacteria. Phytophthora nicotianae is inoculated at the center of the PDA medium, and a colony with a radius of 5 mm is obtained. Then, two perpendicular lines with a length of 5 cm are drawn from the center point of the plate as the intersection, and the isolated bacteria are inoculated at the four end points. The control group is inoculated with Phytophthora nicotianae only, and the isolated bacteria are not inoculated. Each treatment is repeated three times. The mixture is cultured at 30°C, and the antagonistic effect of the isolated bacteria is observed when the colonies of the control group cover the plate.
[0041] According to the above plate confrontation test, the strain with the best antagonistic effect is selected, and the inhibition rate of Phytophthora nicotianae is 66.2%. The strain is named LGWZYY-008.
[0042] Example 2
[0043] Strain identification
[0044] 1. Observation of colony morphology
[0045] The colony of strain LGWZYY-008 on the agar plate is medium-sized, irregular, smooth-edged, and low convex with a raised middle. The colony surface is rough and red Figure 1 .
[0046] 2. Identification of physiological and biochemical characteristics of the strain
[0047] Strain identification is one of the most important steps in biocontrol bacteria research and production. Reliable strain identification must be carried out before antagonistic bacteria are produced and applied. Traditional strain identification methods start from the shape, size, surface characteristics, color and other aspects of the colony, and determine the taxonomic status of a certain strain through a series of physiological and biochemical reaction tests. Different microorganisms have different enzyme systems and different types of metabolism, so the metabolic products produced after the utilization of various substances are also different. Therefore, the physiological and biochemical reactions of microorganisms can be used to determine the metabolic products of microorganisms, and then identify microorganisms that are difficult to distinguish in morphology.
[0048] The physiological and biochemical identification results of strain LGWZYY-008 are shown in Table 1:
[0049] Table 1 Physiological and biochemical characteristics of antagonistic bacteria LGWZYY-008
[0050] Note: "+" indicates a positive reaction; "-" indicates a negative reaction.
[0051] The results of this physiological and biochemical test show that strain LGWZYY-008 has the ability to produce amylase, which can decompose starch into maltose and glucose and then be used by microorganisms as a carbon source; the ability to produce IAA, which can affect root structure, nutrient absorption, and resistance to various abiotic stresses (such as drought, salinity, and heavy metal toxicity), and enhance the resistance of plants; the ability to decompose and utilize casein; the ability to produce siderophores, which can help plants better absorb iron elements in the soil and inhibit the growth of pathogenic bacteria; the content of biofilm affects the resistance of plants to biological and abiotic stresses, and directly promotes the nutrient absorption and root development of plants.
[0052] 3. Molecular biology identification of the strain
[0053] The DNA of strain LGWZYY-008 was extracted using a bacterial genome extraction kit, and PCR amplification was performed using 16S universal primers. The PCR product was sequenced by Shengong Bioengineering (Shanghai) Co., Ltd., and the sequencing results are as follows.
[0054]
[0055] After sequencing, the length of the 16S rDNA gene sequence of the strain LGWZYY-008 is 1462 bp. In the NCBI database, it is found that the 16S rDNA sequence of the strain LGWZYY-008 has the highest similarity of 100% with the strain Streptomyces sp. 3214.6.
[0056] Example 3
[0057] Preservation of the strain
[0058] The strain Streptomyces LGWZYY-008, which is classified as Streptomyces sp., was preserved in the China Center for Type Culture Collection on September 3, 2025, with the preservation number CCTCC NO: M20251957 and the preservation address being Wuhan, China, Wuhan University.
[0059] Example 4
[0060] Exploration of the optimal growth environment conditions of the strain Streptomyces LGWZYY-008
[0061] 1. Optimal temperature test
[0062] Inoculate the strain Streptomyces LGWZYY-008 in NB medium and place it in different temperatures (20℃, 30℃, 37℃, 41℃, 45℃, 60℃) for 24 h, then observe the growth of the bacteria by naked eye.
[0063] 2. Optimal pH test
[0064] Adjust the NB medium to different pH (5, 6, 7, 8, 9), inoculate the strain Streptomyces LGWZYY-008, and then place it at 30℃ for 24 h, then measure the OD 600 .
[0065] 3. Optimal inorganic salt test
[0066] Add different inorganic salts (sodium chloride, magnesium sulfate, potassium sulfate) 10 g / L in NB medium, inoculate the strain Streptomyces LGWZYY-008, and then place it at 30℃ for 24 h, then measure the OD 600 .
[0067] 4. Best carbon source test
[0068] Add different carbon sources (glucose, beef extract, sucrose) 10 g / L in inorganic salt medium, inoculate the strain Streptomyces LGWZYY-008, and then place it at 30℃ for 24 h, then measure the OD 600 .
[0069] 5. Optimum nitrogen source test
[0070] Different nitrogen sources (10 g / L of yeast powder, peptone, and tryptone) were added to NB medium, and the medium was inoculated with Streptomyces LGWZYY-008 and incubated at 30°C for 24 h. Then, the OD of the culture solution was measured 600 .
[0071] The test results are shown in Table 2.
[0072] Table 2. Results of optimum growth environment condition exploration
[0073] Example 5
[0074] Antagonistic effect of Streptomyces LGWZYY-008 on typical plant pathogenic fungi
[0075] The antagonistic test was performed on pathogenic fungi of tobacco, eggplant, cucumber, and tomato using Streptomyces LGWZYY-008.
[0076] First, Streptomyces LGWZYY-008 was streaked on NB plate medium and incubated at 30°C for 24 h for standby. Then, each pathogenic fungus strain was cultured on PDA plate medium and incubated at 27°C for 5 d for standby.
[0077] The treatment group was prepared by punching a fungus cake in the center of the pathogenic fungus colony using a 5-mm puncher and placing it in the center of a new PDA plate medium, and simultaneously, a single colony of Streptomyces LGWZYY-008 was picked up using a bamboo stick and streaked on both sides of the PDA plate medium (each treatment was repeated three times).
[0078] The control group was prepared by inoculating only the PDA medium plate with each pathogenic fungus.
[0079] The inhibition rate (%) = (diameter of the control group - diameter of the treatment group) / diameter of the control group × 100%.
[0080] The antagonistic effect of Streptomyces LGWZYY-008 on typical plant diseases is shown in Table 3.
[0081] Table 3. Antagonistic effect on typical plant diseases
[0082] The results show that the Streptomyces LGWZYY-008 has different degrees of disease resistance effect on tobacco and other crops such as pepper, tomato, eggplant, cucumber, wild rice shoot, strawberry and corn; among them, the inhibition effect on tobacco target spot and yellow carbon anthracnose is the best, with a bacteriostatic rate of more than 70%, followed by the inhibition effect on wild rice shoot laceration, tomato leaf spot, cucumber laceration, with a bacteriostatic rate of more than 55%, and the inhibition effect on corn blight is poor, with a bacteriostatic rate of only 18.33%; thus, the Streptomyces LGWZYY-008 is a biocontrol strain with a wide spectrum of inhibition.
[0083] Example 6
[0084] Application of Streptomyces LGWZYY-008 in tobacco growth promotion and disease resistance
[0085] The test is a substrate potting test, and six treatments are set, each with 9 repeats, which are as follows:
[0086] Control group:
[0087] ① CK: no nitrogen fertilizer is added, and only tobacco Phytophthora infestans is inoculated;
[0088] ② N7: 7 kg of nitrogen fertilizer is added per mu, and only tobacco Phytophthora infestans is inoculated;
[0089] ③ N10: 10 kg of nitrogen fertilizer is added per mu, and only tobacco Phytophthora infestans is inoculated.
[0090] Treatment group:
[0091] ④ CK+PR008: inoculate tobacco Phytophthora infestans and Streptomyces LGWZYY-008 under the condition of no nitrogen fertilizer addition;
[0092] ⑤ N7+PR008: inoculate tobacco Phytophthora infestans and Streptomyces LGWZYY-008 under the condition of 7 kg / mu of nitrogen fertilizer addition;
[0093] ⑥ N10+PR008: inoculate tobacco Phytophthora infestans and Streptomyces LGWZYY-008 under the condition of 10 kg / mu of nitrogen fertilizer addition.
[0094] In each of the above treatments, the application rates of P and K fertilizers are consistent, the application rate of P2O5 is 10.5 kg / mu, and the application rate of K2O is 29.4 kg / mu.
[0095] The tobacco variety in the test is Yunyan 87, and the test potting substrate is high-temperature sterilized treatment, and the tobacco seedlings come from Leiyang Tobacco Bureau.
[0096] The inoculation method of tobacco Phytophthora infestans is as follows: taking the transplanting of tobacco seedlings as 0 day, and carrying out root irrigation treatment at 28, 35 and 49 days, respectively, with a dosage of 10 mL (the concentration of pathogenic spore solution is 1×10 4 ).
[0097] Preparation method of Streptomyces LGWZYY-008 bacterial solution: take 100 μL of single-strain glycerol bacteria, add 250 mL of LB liquid medium, cultivate at 30 ℃ and 220 rpm for 24 h, centrifuge at 4000 r / min for 10 min, suspend the bacterial cells in sterile deionized water, and adjust the OD value to 1.2, take out after cultivation in a 30 ℃ constant temperature incubator for 7 days, and then dilute with sterile distilled water to obtain a bacterial solution containing 1×10 600 7 cfu / mL of viable bacteria for standby.
[0098] Application method of Streptomyces LGWZYY-008 bacterial solution: take the tobacco seedlings as 0 days, and perform root irrigation treatment at 7, 14, 28, 35, 49 and 63 days, with a dosage of 50 mL per plant.
[0099] After the tobacco seedlings are transplanted for 90 days, the incidence of tobacco black shank (disease control effect) is counted, and the disease control effect is calculated, and the calculation formula is as follows:
[0100] Disease control effect = (disease incidence of the control group - disease incidence of the treatment group) / disease incidence of the control group.
[0101] The agronomic trait indexes (plant height, stem diameter, leaf length, leaf width, SPAD, and biomass) of tobacco are measured, the agronomic traits of tobacco after transplantation are determined according to the standard method of YC / T 142-2010, the SPAD-502 (Konica Minolta) is used to determine the SPAD value of the middle tobacco leaf in each treatment, and the average value is calculated by repeating three times.
[0102] The disease resistance and growth promotion effects of the test are as shown in Tables 4-10:
[0103] Table 4 Disease incidence and control effect
[0104] Table 5 Plant height
[0105] Table 6 Stem diameter
[0106] Table 7 Maximum leaf length
[0107] Table 8 Maximum leaf width
[0108] Table 9 SPAD
[0109] Table 10 Above-ground biomass
[0110] As shown in Tables 4-10, under different nitrogen fertilizer concentration gradients, Streptomyces LGWZYY-008 has good control effect on tobacco black shank disease. Under the background of no nitrogen fertilizer and 7 kg / mu of nitrogen fertilizer, the control effect of Streptomyces LGWZYY-008 on tobacco black shank disease reaches 75%. Among the tobacco agronomic trait indexes, under the addition of 7 kg of nitrogen fertilizer per mu, Streptomyces LGWZYY-008 has the best effect on tobacco indexes, with an increase of 18.67% in plant height, 9.08% in stem diameter, 10.81% in maximum leaf width, and 15.91% in above-ground biomass. This shows that Streptomyces LGWZYY-008 can promote the growth and development of tobacco plants, and the fresh weight and weight of the plants are improved, significantly reducing the inhibitory effect of the pathogen on tobacco growth.
[0111] Example 7
[0112] Root enzyme activity determination after applying Streptomyces LGWZYY-008 to tobacco pots
[0113] To explore the effect of Streptomyces LGWZYY-008 on root enzyme activity, the root activity, malondialdehyde (MDA), and peroxidase (POD) of tobacco were determined. Six treatments were set up, with 9 replicates for each treatment, which were:
[0114] Control group:
[0115] ① CK: no addition of nitrogen fertilizer, only inoculation of tobacco Phytophthora fungus;
[0116] ② N7: addition of 7 kg of nitrogen fertilizer per mu, only inoculation of tobacco Phytophthora fungus;
[0117] ③ N10: addition of 10 kg of nitrogen fertilizer per mu, only inoculation of tobacco Phytophthora fungus.
[0118] Treatment group:
[0119] ④ CK+PR008: inoculation of tobacco Phytophthora fungus and Streptomyces LGWZYY-008 under the condition of no addition of nitrogen fertilizer;
[0120] ⑤ N7+PR008: inoculation of tobacco Phytophthora fungus and Streptomyces LGWZYY-008 under the condition of addition of 7 kg of nitrogen fertilizer per mu;
[0121] ⑥ N10+PR008: inoculation of tobacco Phytophthora fungus and Streptomyces LGWZYY-008 under the condition of addition of 10 kg of nitrogen fertilizer per mu.
[0122] In each of the above processes, the P and K fertilizer application rates remain the same, with P2O5 at 10.5 kg / acre and K2O at 29.4 kg / acre.
[0123] The test tobacco variety was Yunyan 87, and the test potting substrate was all subjected to high-temperature sterilization treatment, and the tobacco seedlings came from Leiyang Tobacco Bureau.
[0124] Tobacco Phytophthora inoculation method: at 0 days (when the tobacco seedlings were transplanted), 28 days, 35 days, and 49 days, respectively, root irrigation treatment was performed, with a dosage of 10 mL per plant (the concentration of pathogen spore solution was 1×10 4 ).
[0125] Preparation method of Streptomyces LGWZYY-008 bacterial solution: take 100 μL of single-strain glycerol bacteria, add 250 mL of LB liquid medium, cultivate at 30 ℃ and 220 rpm for 24 h, centrifuge at 4000 r / min for 10 min, suspend the bacterial cells in sterile deionized water, and adjust the OD 600 value to 1.2, take out after 7 days of cultivation in a 30 ℃ constant temperature incubator, and then dilute with sterile distilled water to prepare a bacterial solution containing 1×10 7 cfu / mL of viable bacteria for standby use.
[0126] Application method of Streptomyces LGWZYY-008 bacterial solution: at 0 days (when the tobacco seedlings were transplanted), 7 days, 14 days, 28 days, 35 days, 49 days, and 63 days, respectively, root irrigation treatment was performed, with a dosage of 50 mL per plant.
[0127] After the tobacco seedlings were transplanted for 90 days, TTC colorimetry was used to determine the root activity of mature tobacco plants, microplate method was used to determine the peroxidase (POD) content, and micro method was used to determine the malondialdehyde (MDA) content.
[0128] The results are shown in Tables 11-13.
[0129] Table 11 Root activity of tobacco
[0130] Table 12 Peroxidase
[0131] Table 13 Malondialdehyde
[0132] Table 11-Table 13 show that when 7 and 10 kg / acre of nitrogen fertilizer is applied, the Streptomyces LGWZYY-008 can increase the tobacco root activity by 143.48% and 205.77% respectively; when 7 kg / acre of nitrogen fertilizer is applied, the Streptomyces LGWZYY-008 can increase the tobacco root peroxidase content by 88.21%; when 10 kg / acre of nitrogen fertilizer is applied, the Streptomyces LGWZYY-008 can reduce the tobacco root malondialdehyde content by 10.33%. The lower the malondialdehyde content in the root, the less the tobacco root is damaged, and the higher the peroxidase content, the stronger the stress resistance of the tobacco root. In summary, the addition of Streptomyces LGWZYY-008 can increase the stress resistance of tobacco plants, improve the adaptability to the external environment, and protect the tobacco plants from the damage of the external environment.
[0133] The various embodiments in the specification are described in a progressive manner, and each embodiment focuses on the difference from other embodiments. The same or similar parts between the embodiments can be referred to each other.
[0134] The above description of the disclosed embodiments enables a person skilled in the art to implement or use the present application. Various modifications to the embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. Streptomyces sp. LGWZYY-008, characterized in that, The classification name is Streptomyces sp. Streptomyces sp. , and was preserved in the China Center of Type Culture Collection on September 3, 2025, with a preservation number of CCTCC NO: M20251957 and a preservation address of Wuhan, China.
2. The streptomycete LGWZYY-008 of claim 1 is used in the prevention and treatment of plant diseases.
3. Use according to claim 2, wherein the compound is ###0002### The plants are tobacco, pepper, tomato, polygonatum, eggplant, cucumber, zizania aquatica, strawberry and corn.
4. The use according to claim 2, wherein the compound is ###0002### The plant diseases are cucumber sharp knife bunt, tobacco sharp cell disease, tobacco target spot disease, tobacco black foot disease, polygonatum anthracnose, strawberry blight, pepper wilt, pepper anthracnose, zizania aquatica bunt, corn blight, tomato leaf spot, tomato early blight and eggplant wilt.
5. The streptomycete LGWZYY-008 of claim 1 is used in the improvement of tobacco resistance to tobacco black foot disease.
6. The streptomycete LGWZYY-008 of claim 1 is used in the promotion of tobacco growth.
7. Use according to claim 6, wherein The promotion of tobacco growth is the improvement of plant height, stem diameter, leaf length, leaf width, SPAD and biomass of tobacco.
8. The streptomycete LGWZYY-008 of claim 1 is used in the improvement of root activity and peroxidase content of tobacco.
9. The streptomycete LGWZYY-008 of claim 1 is used in the reduction of malondialdehyde content of tobacco roots.
10. A tobacco root immune-enhancing bacterial agent, characterized in that, The streptomycete LGWZYY-008 of claim 1 is included.