Natural ecological strain cultivation device for saline-alkali soil improvement

Abstract

The invention discloses a natural ecological strain cultivation device for saline-alkali soil improvement, and belongs to the technical field of strain cultivation, the natural ecological strain cultivation device for saline-alkali soil improvement comprises a supporting bottom bin, and one side of the supporting bottom bin is slidably connected with a plurality of anti-precipitation liquid storage assemblies in a penetrating mode; the top end of the supporting bottom bin is fixedly connected with a sealed bin, the two sides of the sealed bin are fixedly connected with a multi-layer bin assembly, the inner side of the sealed bin is rotatably connected with an irradiation frame, the middle of the inner side of the multi-layer bin assembly is fixedly connected with an isolation assembly, and the outer side of the isolation assembly is rotatably connected with a rotating cabinet. The cold cabin and the hot cabin are partitioned, synchronous linkage of the irradiation frame and the rotating cabinet is combined, the day and night temperature difference in the natural world is simulated, and the real physical stress and the recovery period experienced by saline-alkali soil strains in the natural environment are highly restored through the synergistic change in cooperation with the spraying assembly; therefore, the target strain with higher adaptability and more stable improvement effect can be more efficiently screened and cultivated.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of strain cultivation, and particularly relates to a natural ecological strain cultivation device for saline-alkali soil improvement. BACKGROUND

[0002] The natural ecological strain for saline-alkali soil improvement refers to indigenous microorganisms including bacteria and fungi, which are isolated and screened from natural environments such as saline-alkali soil or salt-tolerant plant roots and have salt-tolerant characteristics. After being cultured and expanded, the strain is applied to saline-alkali soil to improve the physicochemical properties of the soil, enhance the fertility of the soil and promote the growth of plants, so as to achieve ecological restoration. This is currently the most safe, economic and efficient biological improvement measure.

[0003] However, when such a strain is cultivated and functionally studied in a laboratory on a large scale, it faces special challenges. The growth environment needs to simulate the specific conditions of saline-alkali soil, such as salt stress, while providing suitable temperature, humidity, light and nutrients. The general microbial incubator or fermenter in the prior art often has a single function, and its environmental simulation capability is insufficient, which cannot effectively simulate the variable environment required by the strain from adaptation to expansion. SUMMARY

[0004] The technical problem to be solved by the application is to overcome the shortcomings of the prior art and provide a natural ecological strain cultivation device for saline-alkali soil improvement.

[0005] The technical solution adopted to solve the above technical problem is as follows: a natural ecological strain cultivation device for saline-alkali soil improvement, comprising a support bottom bin, a plurality of anti-settling liquid storage assemblies are connected to one side of the support bottom bin in a sliding manner, a sealed cabin is fixedly connected to the top end of the support bottom bin, a plurality of multilayer cabin assemblies are fixedly connected to the two sides of the sealed cabin, a monitoring panel is arranged on the surface of the sealed cabin, an irradiation frame is rotatably connected to the inner side of the sealed cabin, an isolation assembly is fixedly connected to the middle of the inner side of the multilayer cabin assembly, a rotating cabinet is rotatably connected to the outer side of the isolation assembly, a plurality of culture media are slidably connected in the rotating cabinet, and the rotating cabinet and the irradiation frame are rotatably connected to each other.

[0006] Further, two isolation assemblies are fixedly connected to the inner side of the isolation assembly, and a spraying assembly is fixedly connected to the inner side of the isolation assembly.

[0007] Further, the culture medium is provided with a high-density gauze at the bottom end, the culture medium is located between the spraying assemblies, and the spraying assemblies are fixedly connected to the anti-settling liquid storage assemblies.

[0008] Through the above technical solution, the high-density gauze is arranged to allow the salt solution at the spraying end to slowly penetrate upward, thereby simulating the salt environment in saline-alkali soil, and the arrangement of the anti-settling liquid storage assembly ensures the accuracy of the liquid concentration.

[0009] Further, the isolation assembly comprises a stand column, the bottom end of the stand column is fixedly connected with the support bottom bin, the middle part of the stand column is fixedly connected with an annular hopper, the two sides of the annular hopper are fixedly connected with the air inlet assembly respectively, the top end of the annular hopper is fixedly connected with T-shaped isolation plates on the two sides and the inner side of the stand column respectively, the outer side of the T-shaped isolation plate is fixedly connected with the spraying assembly, the two sides of the stand column are divided into a cold cabin and a hot cabin by the T-shaped isolation plate, the outer wall of the T-shaped isolation plate is rotationally attached to the inner wall of the rotary cabinet, and the cold cabin and the hot cabin are in communication with the inside of the rotary cabinet respectively.

[0010] Through the above technical scheme, the T-shaped isolation plate and the stand column divide the annular space of the rotary cabinet into two halves, forming independent cold cabin and hot cabin for partition temperature control, ensuring that the airflow of two different temperatures can be strictly limited in the respective area, minimizing the mixing of cold and hot air, providing a foundation for creating a clear and stable temperature gradient. When the culture medium rotates with the rotary cabinet, it can periodically and sequentially pass through the cold cabin and the hot cabin, thereby simulating the day-night alternating environment, which is more suitable for the natural growth environment of the strain, and improving the survival rate of the strain after transplanting.

[0011] Further, a plurality of compartments are arranged inside the rotary cabinet, a blocking strip is arranged on the two sides of each compartment, and the top end of the blocking strip is in sliding connection with the culture medium.

[0012] Further, the irradiation frame comprises a rotating rod, a second motor is rotationally connected to the bottom end of the rotating rod, the power output end of the second motor is fixedly connected with the support bottom bin, a plurality of six-paw turntables are fixedly connected in an up-down array on the middle part of the rotating rod, a plurality of irradiation lamps are arranged at the bottom end of each six-paw turntable, the irradiation lamps of the same layer are rotationally corresponding to the compartments of the same layer, a double-groove spool is fixedly connected to the top end of the rotating rod, two belts are frictionally connected to the surface of the double-groove spool respectively, and the two belts are frictionally connected to the single spool respectively.

[0013] Through the above technical scheme, the motor drives the irradiation frame and the rotary cabinet to rotate synchronously through the spool belt structure, and the staggered rotation movement enables the irradiation lamps to simulate the sun's irradiation angle. The six-paw turntable on the irradiation frame rotates by a specific angle, thereby changing the incident angle of light or simulating the photoperiod change of sunrise and sunset, simulating the real day-night cycle and alternating environment of saline-alkali land, thereby efficiently screening and cultivating adaptive strains.

[0014] Further, the spraying assembly comprises a plurality of annular sleeves, a sealing annular plate is rotatably connected to the outside of the annular sleeve, an extension pipe is mounted on the surface of the sealing annular plate, a nozzle is arranged at the end of the extension pipe away from the sealing annular plate, a plurality of annular sleeves are arranged on the upper and lower ends of the inner side of each compartment respectively, the two nozzles in the same compartment are oppositely arranged, a plurality of annular sleeves on the top end of the inner side of the compartment are fixedly connected to the inner side of the upper connecting pipe, and a plurality of annular sleeves on the bottom end of the inner side of the compartment are fixedly connected to the inner side of the lower connecting pipe. The lower connecting pipe and the upper connecting pipe are fixedly connected with the anti-deposition liquid storage assembly respectively.

[0015] Through the above technical scheme, the annular sleeve and the sealing annular plate cooperate with each other, so that even if the rotating cabinet drives the culture medium to rotate continuously, the nozzle can still maintain the basic stability of its spatial orientation through the relative rotation of the sealing annular plate, ensuring that the spraying direction is always aligned with the culture area. At the same time, the upper and lower nozzles create a three-dimensional spraying effect. The lower nozzle can simulate the direction and intensity of salt stress, and the upper nozzle simulates the irrigation effect in the real environment. This not only realizes fine adjustment of the spraying intensity and mode, but also simulates the growth environment of the fungus in the real environment, further improving the fungus cultivation effect.

[0016] Further, the air inlet assembly comprises a first pipeline and a second pipeline, the first pipeline is fixedly communicated with the cold cabin, a circulating air duct is fixedly connected to the middle part of the first pipeline, a semiconductor heat dissipation block is fixedly connected to the inside of the circulating air duct, one end of the semiconductor heat dissipation block is fixedly connected with the second pipeline, the circulating air duct is fixedly connected with the supporting bottom bin, a first electric fan is installed on the penetrating end of the circulating air duct, the second pipeline is fixedly communicated with the hot cabin, an air inlet duct is fixedly connected with the middle part of the first pipeline and the middle part of the second pipeline, a second electric fan is fixedly connected to the inside of the air inlet duct, the air inlet duct is fixedly connected with the supporting bottom bin, and a filter screen is installed on the penetrating end of the air inlet duct.

[0017] Through the above technical scheme, the air inlet duct delivers the filtered air into the first pipeline and the second pipeline, and the circulating air duct cools the semiconductor heat dissipation block through the flowing air, thereby cooling the air in the first pipeline and achieving the cooling effect of the cold cabin, simulating the night cooling environment. After the air in the second pipeline enters the hot cabin, it is heated by the irradiation lamp, simulating the daytime warming environment, and achieving precise simulation of day and night alternation.

[0018] Further, the anti-precipitation liquid storage assembly comprises a pull plate, the inner side of the pull plate is fixedly connected with a stand, the bottom ends of the two sides of the stand are slidably connected with fixed carriages, the fixed carriages are fixedly connected with the supporting bottom bin, the top end of the side close to the pull plate of the stand is rotatably connected with a fixed frame, the inner side of the fixed frame is slidably connected with square buckets, the inner parts of the square buckets are respectively provided with clean water, brine and nutrient solution, the bottom end of the side away from the pull plate of the fixed frame is fixedly connected with an extension plate, one end of the extension plate is slidably connected with a roller, the roller is slidably connected with the fixed carriages, the penetrating end of the roller is rotatably connected with a push handle, the bottom end of the side of the fixed carriage is fixedly connected with a plurality of mounting sleeves, the mounting sleeves are rotatably connected with a transmission rod, the middle part of the transmission rod is fixedly connected with a plurality of cams, the surfaces of the cams are slidably attached to the surface of the push handle, one end of the transmission rod is rotatably connected with a first motor, the power output end of the first motor is fixedly connected with the transmission rod, the first motor is fixedly connected with the fixed carriage, the top end of the side close to the fixed carriage of the stand is fixedly connected with a pump, the water inlet of the pump is located at the inner bottom end of the square bucket, the water outlet of the pump connected with the square bucket provided with clean water and nutrient solution is fixedly connected with a mixing tank, the mixing tank is fixedly connected with an upper connecting pipe, and the water outlet of the pump connected with the square bucket provided with brine is fixedly connected with a lower connecting pipe.

[0019] Through the above technical scheme, the motor drives the cam to rotate, when the roller is attached to the cam, the push handle continuously pushes the bottom of the square bucket to shake, the low-amplitude and high-frequency shaking can effectively prevent the solute from settling, ensures that the concentration of the solution in the barrel is uniform at all times, is conducive to long-term cultivation, reduces manual operation, the system can instruct the mixing tank to pump nutrient solution for spraying, and can also pump brine for stress, the change of the chemical environment is a simulation of the real saline-alkali ecological conditions, and the effectiveness of the strain cultivation is ensured.

[0020] Further, the multi-layer cabin assembly comprises a semicircular sleeve, the semicircular sleeve is fixedly connected with a sealed cabin, the inner wall of the sealed cabin is rotatably attached to a rotating cabinet, the middle part of the semicircular sleeve is slidably connected with a sealing door, the middle part of the sealing door is provided with an observation window, one side of the surface of the observation window is rotatably connected with a light shield, and the outer surface of the semicircular sleeve is slidably connected with an arc-shaped door.

[0021] Through the above technical scheme, a double-layer safety structure is formed, and the rotating cabinet is rotated, so that each compartment can be individually observed and accessed, the damage to the internal environment caused by observation and access operations is reduced, and the stability of the internal environment is ensured.

[0022] The beneficial effects of the present application are as follows: 1. The present application is a cultivation core that can accurately simulate dynamic complex environments by integrating the isolation assembly with the air inlet assembly, the rotating cabinet, and the spraying assembly. The partitioning of the cold cabin and the hot cabin, combined with the periodic rotation of the rotating cabinet, allows the culture medium to automatically undergo programmed temperature cycles, simulating the day-night and seasonal temperature differences in nature. At the same time, the synchronization of the illumination rack and the rotating cabinet achieves the simulation of light angle and cycle. This coordinated change in temperature and illumination in space and time highly restores the real physical stress and recovery period experienced by the saline-alkali soil bacteria in the natural environment, thereby more efficiently screening and cultivating target bacteria with stronger adaptability and more stable improvement effect.

[0023] 2. The present application significantly improves the cultivation efficiency and operational convenience through highly automated and modular design. From the anti-settling storage of liquid, precise pumping, to the temperature-controlled circulation of environmental gas, the periodic change of illumination, to the uniform rotation of the culture unit, the whole process can be controlled by a monitoring panel, achieving fully automated operation and minimizing human intervention and pollution risk. At the same time, the observation window and sealed door of the multi-layer cabin assembly facilitate non-destructive observation and sampling; the drawer design of the anti-settling liquid storage assembly facilitates maintenance; the modularization of the culture medium makes replacement simple and fast, making the device not only suitable for rigorous laboratory research, but also has the potential to be applied to large-scale and standardized production. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 is the first structural schematic diagram of the present application; Figure 2 is the second structural schematic diagram of the present application; Figure 3 is the internal structure of the present application; Figure 4 is the overall internal structure of the present application; Figure 5 is the installation structure of the isolation assembly and air inlet assembly of the present application; Figure 6 is the structural front view of the rotating cabinet of the present application; Figure 7 is the installation structure of the spraying assembly of the present application; Figure 8 is the local structure of the spraying assembly of the present application; Figure 9 is the air inlet duct structure of the present application; Figure 10 is the culture medium structure of the present application Figure 11 is the overall structure of the anti-settling liquid storage assembly of the present application Figure 12It is a partial structure perspective view of the anti-settling liquid storage assembly of the present application.

[0025] Reference signs: 1, support bottom bin; 2, anti-settling liquid storage assembly; 201, pull plate; 202, square bucket; 203, fixed frame; 204, stand; 205, fixed carriage; 206, pump; 207, mixing tank; 208, transmission rod; 209, mounting sleeve; 210, cam; 211, first motor; 212, extension plate; 213, push handle; 214, roller; 3, sealed cabin; 4, monitoring panel; 5, multi-layer cabin assembly; 501, semicircular sleeve; 502, arc-shaped door; 503, sealed door; 504, light shield; 505, observation window; 6, rotating cabinet; 601, compartment; 602, blocking strip; 603, single spool; 7, culture medium; 701, high-density gauze; 8, isolation assembly; 801, upright column; 802, T-shaped isolation plate; 803, cold cabin; 804, hot cabin; 805, annular hopper; 9, irradiation frame; 901, rotating rod; 902, second motor; 903, six-claw turntable; 904, irradiation lamp; 905, double-groove spool; 906, belt; 10, air inlet assembly; 1001, first pipeline; 1002, second pipeline; 1003, circulating air duct; 1004, air inlet air duct; 1005, first electric fan; 1006, filter screen; 1007, second electric fan; 1008, semiconductor heat sink; 11, spraying assembly; 1101, annular sleeve; 1102, sealed annular plate; 1103, extension pipe; 1104, spout; 1105, lower connecting pipe; 1106, upper connecting pipe. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application is further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0027] As shown in the drawings, Figures 1 to 12 The natural ecological bacteria cultivation device for saline-alkali soil improvement of the present embodiment comprises a support bottom bin 1, a plurality of anti-settling liquid storage assemblies 2 are connected to the support bottom bin 1 through sliding connection on one side, a sealed cabin 3 is fixedly connected to the top end of the support bottom bin 1, a multi-layer cabin assembly 5 is fixedly connected to the two sides of the sealed cabin 3, a monitoring panel 4 is arranged on the surface of the sealed cabin 3, an irradiation frame 9 is rotatably connected to the inner side of the sealed cabin 3, an isolation assembly 8 is fixedly connected to the middle of the inner side of the multi-layer cabin assembly 5, a rotating cabinet 6 is rotatably connected to the outer side of the isolation assembly 8, a plurality of culture media 7 are slidably connected inside the rotating cabinet 6, and the rotating cabinet 6 and the irradiation frame 9 are rotatably connected to each other.

[0028] As shown in the drawings, Figures 1 to 2As shown, the multi-layer cabin assembly 5 includes a semicircular sleeve 501 fixedly connected with the sealed cabin 3, the inner wall of the sealed cabin 3 is rotationally attached to the rotating cabinet 6, the semicircular sleeve 501 is uniformly and slidably connected with a sealing door 503 at the middle part, the sealing door 503 is provided with an observation window 505 at the middle part, the observation window 505 is rotationally connected with a light shield 504 at one side of the surface, and the semicircular sleeve 501 is slidably connected with an arc-shaped door 502 at the outer surface.

[0029] As shown in the figure, Figures 3 to 4 As shown, the irradiation frame 9 includes a rotating rod 901, the bottom end of the rotating rod 901 is rotationally connected with a second motor 902, the power output end of the second motor 902 is fixedly connected with the support bottom cabin 1, a plurality of six-paw turntables 903 are fixedly connected with the rotating rod 901 in an array at the middle part, the bottom end of the six-paw turntable 903 is provided with a plurality of irradiation lamps 904, the plurality of irradiation lamps 904 are rotationally corresponding to the same layer of the compartment 601, in the rotating process, the change of the irradiation angle of the simulated sunlight is simulated, and the dead angle of irradiation is also reduced, the top end of the rotating rod 901 is fixedly connected with a double-groove reel 905, the double-groove reel 905 is frictionally and drivingly connected with two belts 906 respectively at the surface, the two belts 906 are respectively frictionally and drivingly connected with the single reel 603, the two belts 906 are synchronously moved, the transmission structure is simplified, and the cost and the failure rate are reduced.

[0030] As shown in the figure, Figures 2 to 4 As shown, the two isolation assemblies 8 are fixedly connected with a spraying assembly 11 at the inner side, and the two isolation assemblies 8 are fixedly connected with an air inlet assembly 10 at the bottom end, the isolation assembly 8 includes a stand column 801, the bottom end of the stand column 801 is fixedly connected with the support bottom cabin 1, the middle part of the stand column 801 is fixedly connected with a ring-shaped hopper 805, the two sides of the ring-shaped hopper 805 are respectively fixedly connected with the air inlet assembly 10, the two sides of the top end of the ring-shaped hopper 805 are respectively fixedly connected with T-shaped isolation plates 802 at the inner side of the stand column 801, the outer side of the T-shaped isolation plate 802 is fixedly connected with the spraying assembly 11, the stand column 801 and the T-shaped isolation plate 802 of the isolation assembly 8 are not only structural members for separating the cold cabin 803 and the hot cabin 804, but also become a base for installing the spraying ring-shaped sleeve 1101 and a wall surface for guiding airflow, the two sides of the stand column 801 are separated into the cold cabin 803 and the hot cabin 804 by the T-shaped isolation plate 802, the outer wall of the T-shaped isolation plate 802 is rotationally attached to the inner wall of the rotating cabinet 6, the cold cabin 803 and the hot cabin 804 are respectively communicated with the inside of the rotating cabinet 6, forming a stable and closed air conveying and supplying basis, ensuring the structural rigidity and the sealing property of the environment control system.

[0031] As shown in the figure, Figures 4 to 6As shown, the rotating cabinet 6 is internally provided with a plurality of compartments 601, which constitute independent and standardized culture units, realizing batch and modular management of strain culture. The compartments 601 are respectively provided with a baffle 602 on both sides, and the top end of the baffle 602 is slidingly connected with the culture medium 7, for fixing the position of the culture medium 7 and facilitating the storage and access operation of the culture medium 7. The rotating cabinet 6 is fixedly connected with a single spool 603 at the top end, which synchronously moves with the irradiation frame 9, ensuring sufficient illumination effect.

[0032] As shown in the figure, Figures 6 to 8 The spraying assembly 11 includes a plurality of annular sleeves 1101, and the outer side of the annular sleeve 1101 is rotatably connected with a sealing annular plate 1102. The sealing annular plate 1102 is provided with an extension pipe 1103 on the surface, and the extension pipe 1103 is provided with a spray port 1104 at the end away from the sealing annular plate 1102. The plurality of annular sleeves 1101 are arranged on the upper and lower ends of the inner side of each compartment 601. Even if the rotating cabinet 6 drives the culture medium 7 to continuously rotate, the spray port 1104 can still maintain the basic stability of its spatial orientation through the relative rotation of the sealing annular plate 1102, ensuring that the spraying direction is always aligned with the culture area. The two spray ports 1104 located in the same compartment 601 are oppositely arranged, and different liquids are sprayed from the upper and lower directions at the same time, realizing omnidirectional and uniform humidification and nourishment. The inner sides of the plurality of annular sleeves 1101 located at the top end of the inner side of the compartment 601 are fixedly connected with an upper connecting pipe 1106, and the inner sides of the plurality of annular sleeves 1101 located at the bottom end of the inner side of the compartment 601 are fixedly connected with a lower connecting pipe 1105. The lower connecting pipe 1105 and the upper connecting pipe 1106 are respectively fixedly connected with the anti-settling liquid storage assembly 2, and the upper and lower connecting pipes 1105 are respectively connected to different liquid sources of the anti-settling liquid storage assembly 2. In the simulation of the growth-promoting period, nutrient solution can be sprayed from the upper and lower directions at the same time; in the simulation of the stress period, salt water can be sprayed from the lower direction alone to simulate the rising of rhizosphere salt, thereby realizing the simulation of the direction and intensity of salt stress.

[0033] As shown in the figure, Figure 9As shown, the air inlet assembly 10 includes a first pipe 1001 and a second pipe 1002, the first pipe 1001 is fixedly communicated with the cold cabin 803, a circulating air duct 1003 is fixedly connected in the middle of the first pipe 1001, a semiconductor heat sink 1008 is fixedly connected inside the circulating air duct 1003, one end of the semiconductor heat sink 1008 is fixedly connected with the second pipe 1002, the circulating air duct 1003 is fixedly connected with the support bottom bin 1, a first electric fan 1005 is installed at the penetrating end of the circulating air duct 1003, the second pipe 1002 is fixedly communicated with the hot cabin 804, the middle of the second pipe 1002 and the middle of the first pipe 1001 are fixedly connected with an air inlet air duct 1004, a second electric fan 1007 is fixedly connected inside the air inlet air duct 1004, the air inlet air duct 1004 is fixedly connected with the support bottom bin 1, a filter screen 1006 is installed at the penetrating end of the air inlet air duct 1004, forming two independent air ducts and fans, which ensures that the generation and conveying paths of cold air and hot air are completely independent, fundamentally avoids the advance mixing of air flow and temperature interference, and guarantees that the air temperature sent to the cold cabin 803 and the hot cabin 804 has the highest purity and stability.

[0034] As shown in Figure 10 The culture medium 7 is provided with a high-density gauze 701 at the bottom end, which is convenient for salt stress simulation, the culture medium 7 is located between the spraying assembly 11, and the spraying assembly 11 is fixedly connected with the anti-settling liquid storage assembly 2.

[0035] As shown in Figures 11 to 12As shown, the anti-settling liquid storage assembly 2 comprises a pull plate 201, the inner side of the pull plate 201 is fixedly connected with a stand 204, the bottom ends of the two sides of the stand 204 are slidably connected with a fixed slide 205, the fixed slide 205 is fixedly connected with the support bottom bin 1, the top end of the side close to the pull plate 201 of the stand 204 is rotatably connected with a fixed frame 203, the inner side of the fixed frame 203 is slidably connected with a square bucket 202, a plurality of square buckets 202 can be respectively filled with clean water, salt water and nutrient solution, and the plurality of square buckets 202 are independently placed in the fixed frame 203 in a sliding manner and respectively contain different media such as clean water, salt water and nutrient solution. This design facilitates users to individually take out any bucket for cleaning, replenishment or replacement of liquid, realizes modular management of the storage unit, is flexible to operate and avoids cross contamination, the bottom end of the side away from the pull plate 201 of the fixed frame 203 is fixedly connected with an extension plate 212, one end of the extension plate 212 is slidably connected with a roller 214, the roller 214 is slidably connected with the fixed slide 205, the penetrating end of the roller 214 is rotatably connected with a push handle 213, the bottom end of one side of the fixed slide 205 is fixedly connected with a plurality of mounting sleeves 209, the penetrating end of the plurality of mounting sleeves 209 is rotatably connected with a transmission rod 208, a plurality of cams 210 are fixedly connected to the middle part of the transmission rod 208, the surface of the cam 210 is slidably attached to the surface of the push handle 213, one end of the transmission rod 208 is rotatably connected with a first motor 211, the power output end of the first motor 211 is fixedly connected with the transmission rod 208, the first motor 211 is fixedly connected with the fixed slide 205, and the square bucket 202 therein is driven to swing regularly. This low-amplitude and high-frequency shaking can effectively prevent the solute from settling and ensure that the concentration of the solution in the bucket remains uniform at all times, providing a physical guarantee for subsequent precise and quantitative spraying, the top end of the side close to the fixed slide 205 of the stand 204 is fixedly connected with a pump 206, the water inlet of the pump 206 is located in the inner bottom end of the square bucket 202, the water outlet of the pump 206 connected with the square bucket 202 containing clean water and nutrient solution is fixedly connected with a mixing tank 207, the mixing tank 207 is fixedly connected with the upper connecting pipe 1106, and the water outlet of the pump 206 connected with the square bucket 202 containing salt water is fixedly connected with the lower connecting pipe 1105. The water inlet of the pump 206 penetrates the bottom of each bucket to directly extract homogeneous solution. The conveying path is intelligently divided: the pump 206 connected with the mixing tank 207 is responsible for pumping clean water and nutrient solution as needed and mixing them in the tank in real time to form culture solution, which is supplied to the upper spray port 1104 of the spraying assembly 11 through the upper connecting pipe 1106; and the salt water is directly pumped out by the independent pump 206 and supplied to the lower spray port 1104 through the lower connecting pipe 1105. This design realizes independent or mixed supply of liquids with different properties and lays a foundation for simulating complex scenarios such as spraying salt water from the root or spraying nutrient solution from the upper layer under salt stress.

[0036] The working principle of the embodiment is as follows: The bacteria and its substrate are placed into the culture medium 7, and then each culture medium 7 is placed into each compartment 601, at this time, the sealing door 503 and the arc door 502 closing device are closed, Then the device starts, the second motor 902 drives the irradiation frame 9 and the rotating cabinet 6 to rotate slowly through the belt 906, and the culture medium 7 periodically passes through the cold cabin 803 and the hot cabin 804 separated by the isolation assembly 8 with the rotating cabinet 6.

[0037] At the same time, the air inlet assembly 10 starts to work: the second electric fan 1007 sends the filtered fresh air outside into the first pipeline 1001 and the second pipeline 1002 through the air inlet duct 1004. The air flowing through the circulating air duct 1003 is cooled by the semiconductor heat sink 1008 to form cold air into the cold cabin 803; the air flowing through the second pipeline 1002 is sent into the hot cabin 804 and is naturally heated by the irradiation lamp 904, so that the rotating culture medium 7 alternately stays in a low-temperature and a high-temperature environment, simulating the day and night temperature cycle in nature.

[0038] The irradiation lamp 904 on the irradiation frame 9 rotates with the rotating rod 901, and the light thereof sweeps the passing culture medium 7 at a changing angle, simulating the daily cycle change of sunlight.

[0039] The first motor 211 of the anti-settling liquid storage assembly 2 drives the cam mechanism to make each liquid storage barrel continuously swing slightly to prevent the solution from settling. The control system instructs the corresponding pump 206 to work according to the preset program. For example, in the simulation of the plant growth stage, the mixed tank 207 can be controlled to adjust the mixing ratio of water and nutrient solution, and the mixed culture solution is pumped to the upper connecting pipe 1106 of the spraying assembly 11 and is sprayed from the upper spraying port 1104 to humidify and feed the culture medium 7; in the simulation of the stress or night stage, the salt water pump can be controlled to directly pump the salt solution to the lower connecting pipe 1105, mainly to spray the salt solution from the bottom spraying port 1104 to the culture medium 7 to simulate the actual environment of the saline-alkali soil root.

[0040] The user can set the temperature, light cycle, spraying program and other parameters through the monitoring panel 4, and can monitor the growth state of the bacteria in real time through the observation window 505 on the multi-layer cabin assembly 5, and can open the sealing door 503 to access the culture medium 7 when necessary.

[0041] The device realizes the dynamic and composite simulation of the temperature, light, humidity, salt and nutrition of the natural environment of the saline-alkali soil through the precise cooperation of the above-mentioned subsystems, and provides a platform for efficiently cultivating natural ecological bacteria with adaptability.

[0042] The above only describes the preferred embodiments of the present application and is not used to limit the protection scope of the present application.

Claims

1. A natural ecological bacteria cultivation device for saline-alkali soil improvement, comprising a supporting bottom bin (1), characterized in that: The supporting bottom bin (1) penetrates through and is connected with several anti-settling liquid storage assemblies (2) on one side, the supporting bottom bin (1) is fixedly connected with a sealed cabin (3) at the top end, the sealed cabin (3) is fixedly connected with a plurality of cabin assemblies (5) on both sides, the sealed cabin (3) is provided with a monitoring panel (4) on the surface, the sealed cabin (3) is rotatably connected with an irradiation frame (9) on the inner side, the cabin assembly (5) is fixedly connected with an isolation assembly (8) on the inner side in the middle, the isolation assembly (8) is rotatably connected with a rotating cabinet (6) on the outer side, the rotating cabinet (6) is slidably connected with several culture media (7) inside, and the rotating cabinet (6) and the irradiation frame (9) are rotatably connected with each other; Two isolation assemblies (8) are fixedly connected with spraying assemblies (11) on the inner side, and the bottom ends of the two isolation assemblies (8) are fixedly connected with an air inlet assembly (10) in common; The culture medium (7) is provided with a high-density gauze (701) at the bottom end, the culture medium (7) is located between the spraying assemblies (11), and the spraying assemblies (11) are fixedly connected with the anti-settling liquid storage assemblies (2).

2. The natural ecological bacteria culture device for saline-alkali soil improvement according to claim 1, characterized in that, The isolation assembly (8) comprises a stand column (801), the stand column (801) is fixedly connected with the supporting bottom bin (1) at the bottom end, the stand column (801) is fixedly connected with an annular hopper (805) in the middle, the annular hopper (805) is fixedly connected with the air inlet assembly (10) on both sides, respectively, T-shaped isolation plates (802) are fixedly connected with the inner side of the annular hopper (805) on both sides at the top end and with the stand column (801), respectively, the T-shaped isolation plates (802) are fixedly connected with the spraying assemblies (11) on the outer side, the stand column (801) is divided into a cold cabin (803) and a hot cabin (804) by the T-shaped isolation plates (802) on both sides, the outer wall of the T-shaped isolation plate (802) is rotatably attached to the inner wall of the rotating cabinet (6), and the cold cabin (803) and the hot cabin (804) are in communication with the inside of the rotating cabinet (6), respectively.

3. The natural ecological bacteria culture device for saline-alkali soil improvement according to claim 1, characterized in that, The rotating cabinet (6) is provided with a plurality of compartments (601) inside, the compartments (601) are respectively provided with a baffle (602) on both sides, the baffle (602) is slidably connected with the culture medium (7) at the top end, and the rotating cabinet (6) is fixedly connected with a single spool (603) at the top end.

4. The natural ecological bacteria culture device for saline-alkali soil improvement according to claim 1, characterized in that, The irradiation frame (9) comprises a rotating rod (901), the rotating rod (901) is rotatably connected with a second motor (902) at the bottom end, the power output end of the second motor (902) is fixedly connected with the supporting bottom bin (1), a plurality of six-jaw turntables (903) are fixedly connected with the rotating rod (901) in the middle and arranged in an array from top to bottom, a plurality of irradiation lamps (904) are arranged on the bottom end of the six-jaw turntable (903), a plurality of the irradiation lamps (904) are rotationally corresponding to the compartments (601) of the same layer, a double-groove spool (905) is fixedly connected with the rotating rod (901) at the top end, two belts (906) are frictionally and drivably connected with the surface of the double-groove spool (905), respectively, and the two belts (906) are frictionally and drivably connected with the single spool (603), respectively.

5. The natural ecological bacteria culture device for saline-alkali soil improvement according to claim 1, characterized in that, The spray assembly (11) comprises a plurality of annular sleeves (1101), the outer side of the annular sleeve (1101) is rotationally connected with a sealing annular plate (1102), the surface of the sealing annular plate (1102) is mounted with an extension pipe (1103), the end of the extension pipe (1103) away from the sealing annular plate (1102) is provided with a spray port (1104), a plurality of annular sleeves (1101) are arranged on the upper and lower ends of the inner side of each compartment (601), the two spray ports (1104) in the same compartment (601) are oppositely arranged, the inner sides of a plurality of annular sleeves (1101) at the top end of the inner side of the compartment (601) are fixedly connected with an upper connecting pipe (1106), the inner sides of a plurality of annular sleeves (1101) at the bottom end of the inner side of the compartment (601) are fixedly connected with a lower connecting pipe (1105), and the lower connecting pipe (1105) and the upper connecting pipe (1106) are fixedly connected with the anti-deposition liquid storage assembly (2) respectively.

6. The natural ecological bacteria culture device for saline-alkali soil improvement according to claim 1, characterized in that, The air inlet assembly (10) comprises a first pipeline (1001) and a second pipeline (1002), the first pipeline (1001) is fixedly communicated with the cold cabin (803), the middle part of the first pipeline (1001) is fixedly connected with a circulating air duct (1003), the inside of the circulating air duct (1003) is fixedly connected with a semiconductor heat dissipation block (1008), one end of the semiconductor heat dissipation block (1008) is fixedly connected with the second pipeline (1002), the circulating air duct (1003) is fixedly connected with the supporting bottom bin (1) in penetration, the penetrating end of the circulating air duct (1003) is installed with a first electric fan (1005), the second pipeline (1002) is fixedly communicated with the hot cabin (804), the middle part of the second pipeline (1002) and the middle part of the first pipeline (1001) are fixedly connected with an air inlet air duct (1004) together, the inside of the air inlet air duct (1004) is fixedly connected with a second electric fan (1007), the air inlet air duct (1004) is fixedly connected with the supporting bottom bin (1) in penetration, and the penetrating end of the air inlet air duct (1004) is installed with a filter screen (1006).

7. The natural ecological bacteria culture device for saline-alkali soil improvement according to claim 1, characterized in that, The anti-precipitation liquid storage assembly (2) comprises a pull plate (201), the inner side of the pull plate (201) is fixedly connected with a stand (204), the bottom ends of the two sides of the stand (204) are slidably connected with fixed carriages (205), the fixed carriages (205) are fixedly connected with the supporting bottom bin (1), the top end of the side close to the pull plate (201) of the stand (204) is rotatably connected with a fixed frame (203), the inner side of the fixed frame (203) is slidably connected with square buckets (202), the interiors of the plurality of square buckets (202) are respectively provided with fresh water, brine and nutrient solution, the bottom end of the side away from the pull plate (201) of the fixed frame (203) is fixedly connected with an extension plate (212), one end of the extension plate (212) is slidably connected with a roller (214), the roller (214) is slidably connected with the fixed carriages (205), the penetrating end of the roller (214) is rotatably connected with a push handle (213), the bottom end of the side of the fixed carriage (205) is fixedly connected with a plurality of mounting sleeves (209), the penetrating portion of the plurality of mounting sleeves (209) is rotatably connected with a transmission rod (208), the middle portion of the transmission rod (208) is fixedly connected with a plurality of cams (210), the surface of the cam (210) is slidably attached to the surface of the push handle (213), one end of the transmission rod (208) is rotatably connected with a first motor (211), the power output end of the first motor (211) is fixedly connected with the transmission rod (208), the first motor (211) is fixedly connected with the fixed carriage (205), the top end of the side close to the fixed carriage (205) of the stand (204) is fixedly connected with a pump (206), the water inlet of the pump (206) is located in the interior bottom end of the square bucket (202), the water outlet of the pump (206) connected with the square bucket (202) provided with fresh water and nutrient solution is fixedly connected with a mixing tank (207), the mixing tank (207) is fixedly connected with the upper connecting pipe (1106), and the water outlet of the pump (206) connected with the square bucket (202) provided with brine is fixedly connected with the lower connecting pipe (1105).

8. The natural ecological bacteria culture device for saline-alkali soil improvement according to claim 1, characterized in that, The multi-layer cabin assembly (5) comprises a semicircular sleeve (501), the semicircular sleeve (501) is fixedly connected with a sealed cabin (3), the inner wall of the sealed cabin (3) is rotatably attached to a rotating cabinet (6), the middle portion of the semicircular sleeve (501) is slidably connected with a sealing door (503) in an up-down manner, the middle portion of the sealing door (503) is provided with an observation window (505), one side of the surface of the observation window (505) is rotatably connected with a light shield (504), and the outer surface of the semicircular sleeve (501) is slidably connected with an arc-shaped door (502).

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