Food attractant for dichocrocis punctiferalis

Abstract

The invention belongs to the technical field of food attractants, and particularly relates to a dichocrocis punctiferalis food attractant. The invention relates to a food attractant. The food attractant is prepared from alpha-pinene oxide, methyl glucoside caprylate and leaf acetate according to the mass ratio of 1: (1-3): (1-5). According to the food attractant for dichocrocis punctiferalis disclosed by the invention, the specific components alpha-pinene oxide, methyl glucoside caprylate and leaf acetate are combined and proportioned, and the alpha-pinene oxide, the methyl glucoside caprylate and the leaf acetate respectively play roles in three links of smell attraction, nutritional requirements and host recognition, and are mutually promoted and complemented and synergistic, so that the attraction, attraction and gathering effects of the food attractant on dichocrocis punctiferalis adults are improved; the trapping and killing effect can be greatly optimized, powerful support is provided for green and efficient prevention and control of dichocrocis punctiferalis, agricultural production loss can be reduced, and the quality of agricultural products and agricultural ecological safety are guaranteed.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of food attractants, and particularly relates to a peach borer food attractant. BACKGROUND

[0002] The peach borer belongs to the Lepidoptera Pyralidae and is a polyphagous pest with worldwide distribution, which mainly harms various agricultural and forestry crops such as peaches, pears, apples and corns. The larvae of the peach borer damage the tissues of fruits and stems, resulting in yield reduction and quality decline of crops, and even causing absolute loss in severe cases, which brings huge economic losses to agricultural production. In order to effectively control the harm of the peach borer, the comprehensive control strategy combining green control with chemical control is generally adopted in the industry, and the food attractant has become one of the core technologies in the green control system of the peach borer due to the advantages of environmental friendliness, safety to natural enemies and beneficial organisms, and non-pollution to agricultural products.

[0003] The existing peach borer food attractant usually uses plant source extracts, artificially synthesized esters, terpenes or sugar derivatives as active ingredients, and is prepared by direct application of a single component or simple compounding of multiple active ingredients. However, most of the current products only simply mix single or multiple known active ingredients, and lack of in-depth research on the synergistic mechanism among different components, resulting in insufficient attraction activity, limited attraction and aggregation of adult peach borers in the field, and poor trapping effect.

[0004] There is no related report on the combination of alpha-oxopinene, methyl glucoside caprylate and acetic acid leaf alcohol ester for peach borer food attractant.

[0005] The information disclosed in this BACKGROUND section is only for the purpose of increasing the understanding of the overall background of the application, and should not be regarded as acknowledging or implying in any form that this information constitutes prior art that is known to those of ordinary skill in the art. SUMMARY

[0006] The purpose of the present application is to provide a peach borer food attractant to solve the problems of the prior art in the background.

[0007] To achieve the above-mentioned purpose, the present application provides the following technical solutions: A first object of the present application is to provide a food attractant consisting of alpha-oxopinene (CAS: 72936-74-4), methyl glucoside octanoate and acetic leaf alcohol ester (CAS: 3681-71-8) in a mass ratio of 1:1-3:1-5. Among them, alpha-oxopinene as a terpenoid compound has a significant olfactory taxis induction effect on pests such as peach borer and other lepidoptera pests; methyl glucoside octanoate as a sugar derivative not only retains the sugar skeleton of glucose, which can be recognized by pests as a nutrition source signal, but also optimizes the volatility and stability through the octanoate group, which can simulate the nutrition signal of nectar plants in nature, stimulate the feeding tendency of peach borer adults, and supplement the attraction efficiency from the nutrition demand dimension; acetic leaf alcohol ester simulates the green leaf aroma signal of the host plant, accurately triggers the host recognition and tendency behavior of the pest. In this way, the three of them play a role from the aspects of olfactory attraction, nutrition demand and host recognition, mutually promote and complement each other to improve the attraction and aggregation effect of the food attractant on peach borer adults, which can greatly optimize the trapping effect.

[0008] As a preferred, the mass ratio of alpha-oxopinene, methyl glucoside octanoate and acetic leaf alcohol ester is 1:2:3.

[0009] As a preferred, the preparation method of the methyl glucoside octanoate comprises the following steps: S11. Add 194.17g alpha-methyl glucoside (CAS: 97-30-3) and 184.28g methyl octanoate into a container, stir and mix uniformly, then add 1892mL anhydrous toluene, stir and disperse at 800rpm for 10min; then add 1.89g p-toluenesulfonic acid powder, heat to 110℃ at a rate of 2-3℃ / min, maintain a reflux rate of 2-3 drops / s, and reflux for 6h, then cool to room temperature after the reaction is completed; S12. Cool the reaction solution to 30℃ in an ice bath, slowly add 5% sodium carbonate solution to adjust the pH value to 7.0, stand for 30min, collect the organic phase, and add 200mL saturated brine in the organic phase, stir at 300rpm for 10min, stand for 30min, then collect the organic phase, and repeat the washing until the washing liquid pH value is 7.0 and there is no turbidity; S13. Add 5g anhydrous magnesium sulfate, stir at 25℃ and 200rpm for 2h, filter the dried organic phase with a filter paper with a pore size of 10μm, collect the filtrate in a rotary evaporation flask at 60℃, vacuum degree 0.08MPa and 120rpm, recover toluene until there is no distillate in the flask, and obtain a light yellow oily crude product; S14. The crude product is added to a beaker, 2200 mL of a 7:3 volume ratio of ethanol-water mixed solvent preheated to 60 DEG C is added, 60 DEG C, 600 rpm stirring for 30 min, placed in a low temperature constant temperature bath, cooling to 5 DEG C at a rate of 1 DEG C / 10 min, maintaining 5 DEG C for 8 h to precipitate white needle-shaped crystals; S15. The crystals are collected by suction filtration, the crystal surface is rinsed with 50 mL of ice water twice, each time for 1 min, and then vacuum dried at 50 DEG C and a vacuum degree of 0.09 MPa for 4 h to obtain the methyl glucoside caprylate.

[0010] A second object of the present application is to provide a lure core comprising a slow-release carrier and the food lure loaded on the slow-release carrier.

[0011] A third object of the present application is to provide a preparation method of the lure core, comprising the following steps: S21. The food lure is taken in anhydrous n-hexane, stirred at 25 DEG C and 300 rpm for 15 min to prepare a food lure solution with a mass-volume concentration of 8-12%; S22. The slow-release carrier with a diameter of 5-10 mm and a length of 20-50 mm is soaked and washed with anhydrous ethanol for 2 times, each time for 5 min, vacuum dried at 40 DEG C and a vacuum degree of 0.08 MPa for 30 min, and cooled to room temperature; S23. The slow-release carrier is soaked in the food lure solution at 25 DEG C for 4-6 h, taken out and placed in a ventilated and dark place at room temperature to air dry for 30-60 min to obtain the lure core.

[0012] Preferably, the slow-release carrier is natural rubber with a hardness of 40-50 Shore A and a density of 0.92-0.95 g / cm 3 .

[0013] A fourth object of the present application is to provide the use of the food lure or the lure core in the prevention and control of peach borer.

[0014] Compared with the prior art, the present application has the following beneficial effects: The present application improves the attraction and aggregation effect of the food lure on the adult peach borer by the combination of specific components α-oxopinene, methyl glucoside caprylate and acetic acid leaf alcohol ester, which play a role in the three links of olfactory attraction, nutritional demand and host recognition, promote and complement each other, greatly optimize the trapping effect, and provide a powerful support for the green and efficient prevention and control of peach borer, which helps to reduce the loss of agricultural production and protect the quality of agricultural products and the safety of agricultural ecology. DETAILED DESCRIPTION

[0015] The technical solutions of the patent application will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the patent application, rather than all the embodiments. Based on the embodiments in the patent application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the patent application.

[0016] 1. Control effect of different food attractant lures on peach fruit borer 1.1 Food attractant formula See Table 1.

[0017] Table 1 Food attractant formula Formulation Alpha-oxopinene (g) Methyl glucoside caprylate (g) Acetic acid leaf alcohol ester (g) Y01 10 0 0 Y02 0 10 0 Y03 0 0 10 Y04 10 10 0 Y05 10 0 10 Y06 0 10 10 Y07 10 10 10 Y08 10 10 30 Y09 10 10 50 Y10 10 20 10 Y11 10 20 30 Y12 10 20 50 Y13 10 30 10 Y14 10 30 30 Y15 10 30 50 In Table 1, the preparation method of methyl glucoside caprylate includes the following steps: S11. Add 194.17 g of α-methyl glucoside (CAS: 97-30-3) and 184.28 g of methyl caprylate into a container, stir and mix uniformly, then add 1892 mL of anhydrous toluene, stir and disperse at 800 rpm for 10 min; then add 1.89 g of p-toluenesulfonic acid powder, heat to 110°C at a rate of 2-3°C / min, maintain a reflux rate of 2-3 drops / s, and reflux for 6 h; after the reaction is completed, cool to room temperature; S12. Cool the reaction solution to 30°C in an ice bath, slowly add 5% sodium carbonate solution to adjust the pH value to 7.0, stand for 30 min, collect the organic phase, and add 200 mL of saturated brine to the organic phase, stir at 300 rpm for 10 min, stand for 30 min, and collect the organic phase; repeat the washing until the washing liquid has a pH value of 7.0 and is clear; S13. Add 5 g of anhydrous magnesium sulfate, stir at 25°C and 200 rpm for 2 h, filter the dried organic phase with a filter paper with a pore size of 10 μm, and collect the filtrate in a rotary evaporation flask at 60°C, a vacuum degree of 0.08 MPa, and a rotation speed of 120 rpm to recover toluene until there is no distillate in the flask, to obtain a yellowish crude product; S14. Add the crude product into a beaker, add 2200 mL of a mixed solvent of ethanol and water with a volume ratio of 7:3 preheated to 60°C, stir at 60°C and 600 rpm for 30 min, place in a low-temperature constant-temperature bath, and cool to 5°C at a rate of 1°C / 10 min, and maintain 5°C for 8 h to precipitate white needle-shaped crystals; S15. Filter and collect the crystals, rinse the surface of the crystals with 50 mL of ice water twice, each time for 1 min, and then vacuum dry at 50°C and a vacuum degree of 0.09 MPa for 4 h, to obtain the methyl glucoside caprylate.

[0018] 1.2 Preparation of lure The preparation method is as follows: S21. Take the food attractant in Table 1 into anhydrous n-hexane, stir for 15 min at 25℃ and 300 rpm, and prepare a 12% mass-volume concentration solution of the food attractant, respectively; S22. Soak the slow-release carrier with a diameter of 8 mm and a length of 40 mm in anhydrous ethanol for 2 times, 5 min each time, and vacuum dry at 40℃ and a vacuum degree of 0.08 MPa for 30 min, and cool to room temperature; wherein the slow-release carrier is natural rubber with a hardness of Shore A 40 and a density of 0.95 g / cm 3 ; S23. Soak the slow-release carrier in the food attractant solution at 25℃ for 5 h, and after taking it out, air dry at room temperature, ventilated and away from light for 45 min, to obtain the lure core.

[0019] 1.3 Preparation of the trap A water basin type trap was used. Take a plastic basin with an inner diameter of 25 cm and a depth of 10 cm, fill it with 3 / 4 of the inner space with 0.5% washing powder aqueous solution prepared with water and washing powder, and fix the prepared lure core above the center of the water basin at a distance of 1 cm from the water surface.

[0020] 1.4 Field moth trapping test The test site was a peach orchard with tree age, tree height. The traps were hung on branches about 1.5 m above the ground, and the traps at each site were arranged in a straight line with a distance of 20 m between them. The number of peach borer in each trap was counted and cleaned at 7:00 am every day, and the washing powder aqueous solution in the basin was replenished at the same time. The position of the trap was changed every 2 days to reduce the influence of the hanging position on the trapping effect. A water basin type trap without lure core was set as a blank control, and each treatment was repeated 3 times. The total amount of attraction and the cumulative amount of attraction for 14 days were observed and counted on the 3rd, 7th, 10th and 14th day, and the results are shown in Table 2.

[0021] Table 2 Attraction effect of different food attractant formulations on peach borer As can be seen from Table 2, the blank control CK has no attraction effect on peach borer. Formulations Y01-Y15 can be used for the attraction of peach borer. Compared with Y01-Y06, the combination of α-pinene oxide, methyl glucoside caprylate and acetic leaf alcohol ester in Y07-Y15 significantly improves the attraction effect on peach borer, indicating that the combination of the three can improve the attraction effect on peach borer. By comparing Y07-Y15, it is found that the ratio of α-pinene oxide, methyl glucoside caprylate and acetic leaf alcohol ester also has a significant influence on the attraction effect, among which the formulation Y11 (i.e. the mass ratio of α-pinene oxide, methyl glucoside caprylate and acetic leaf alcohol ester is 1:2:3) has the best attraction effect on peach borer, with a total amount of attraction of 363.

[0022] In summary, the application improves the attraction and aggregation effect of the food attractant on peach borer adults by the combination of specific components α-oxidedipentene, methyl glucoside caprylic acid ester and acetic acid leaf alcohol ester, which play a role in three links of olfactory attraction, nutritional demand and host recognition, promote each other, complement each other, and greatly optimize the trapping effect, providing strong support for the green and efficient prevention and control of peach borer, which helps to reduce agricultural production loss and protect agricultural product quality and ecological safety.

[0023] The foregoing description of specific exemplary embodiments of the application is intended to be illustrative only and is not intended to limit the application to the precise forms described. Many modifications and variations are possible in light of the above teachings without departing from the spirit or essential characteristics of the present application. The exemplary embodiments were chosen and described in order to explain the principles of the application and its practical application and to allow others skilled in the art to understand the application for various exemplary embodiments and its various modifications as are suited to the particular use contemplated. It is intended that the scope of the application be defined by the claims and their equivalents.

Claims

1. A food attractant, characterized in that, The food attractant is composed of α-pinene oxide, methyl glucoside octanoate and acetic leaf alcohol ester in a mass ratio of 1:1-3:1-5.

2. The food attractant of claim 1, wherein The mass ratio of the α-pinene oxide, methyl glucoside octanoate and acetic leaf alcohol ester is 1:2:

3.

3. The food attractant of claim 1, wherein The preparation method of the methyl glucoside octanoate comprises the following steps: S11. In a container, 194.17g of α-methyl glucoside and 184.28g of methyl octanoate are added, and after stirring and mixing uniformly, 1892mL of anhydrous toluene is added, and stirring is carried out at 800rpm for 10min; then 1.89g of p-toluenesulfonic acid powder is added, and the temperature is raised to 110℃ at a rate of 2-3℃ / min, and a reflux rate of 2-3 drops / s is maintained for reflux reaction for 6h, and after the reaction is completed, the temperature is cooled to room temperature; S12. The reaction solution is cooled to 30℃ in an ice bath, a 5% sodium carbonate solution is slowly added to adjust the pH value to 7.0, and after standing for 30min, the organic phase is collected, 200mL of saturated brine is added to the organic phase, stirring is carried out at 300rpm for 10min, and after standing for 30min, the organic phase is collected, and the washing is repeated until the washing liquid has a pH value of 7.0 and is clear; S13. 5g of anhydrous magnesium sulfate is added, stirring is carried out at 25℃ and 200rpm for 2h, the dried organic phase is filtered with a filter paper with a pore size of 10μm, and the filtrate is collected in a rotary evaporation flask at 60℃, a vacuum degree of 0.08MPa and a rotation speed of 120rpm to recover toluene until there is no distillate in the flask, and a light yellow oily crude product is obtained; S14. The crude product is added to a beaker, 2200mL of an ethanol-water mixed solvent with a volume ratio of 7:3 preheated to 60℃ is added, stirring is carried out at 60℃ and 600rpm for 30min, the temperature is lowered to 5℃ at a rate of 1℃ / 10min in a low-temperature constant-temperature bath, and white needle-shaped crystals are precipitated by standing at 5℃ for 8h; S15. The crystals are collected by suction filtration, the crystal surface is washed with 50mL of ice water twice, each time for 1min, and then vacuum drying is carried out at 50℃ and a vacuum degree of 0.09MPa for 4h, and the methyl glucoside octanoate is obtained.

4. A lure core characterized in that, The slow-release carrier and the food attractant loaded on the slow-release carrier according to any one of claims 1-3.

5. A method of making the lure of claim 4, wherein, The method comprises the following steps: S21. The food attractant is taken in anhydrous n-hexane, stirring is carried out at 25℃ and 300rpm for 15min, and a food attractant solution with a mass-volume concentration of 8-12% is prepared; S22. The slow-release carrier with a diameter of 5-10mm and a length of 20-50mm is soaked and washed with anhydrous ethanol for 2 times, each time for 5min, vacuum drying is carried out at 40℃ and a vacuum degree of 0.08MPa for 30min, and the temperature is cooled to room temperature; S23. The slow-release carrier is soaked in the food attractant solution at 25℃ for 4-6h, and after being taken out, it is placed at room temperature, ventilated and away from light for 30-60min, and the lure core is obtained.

6. The preparation method according to claim 5, characterized in that, The slow release carrier is natural rubber, hardness Shore A 40-50, density 0.92-0.95 g / cm 3 .

7. The food attractant according to claim 1 or the lure core according to claim 4 is used for preventing and treating peach weevils.

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