Application of adenine in promoting corn growth
By applying adenine solution to the maize roots, the rhizosphere microbial community was reshaped in a targeted manner, which solved the problem of low growth vigor in maize seedlings and significantly promoted vegetative growth and biomass accumulation, meeting the requirements of green agriculture.
Patent Information
- Application Number
- CN202511513161.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-22
- Publication Date
- 2026-01-23
Smart Images
Figure CN121369403A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the field of microorganisms and agricultural technology, and particularly relates to application of adenine in promoting growth of corn. BACKGROUND
[0002] The crop rhizosphere is a complex micro-ecosystem, and the microbial community (rhizosphere microbiome) therein plays a crucial role in the health, nutrient absorption and stress resistance of the host. Existing studies have shown that different genotypes of crops shape different rhizosphere microbial community structures. However, most studies only stay at the level of correlation analysis, and there is still a lack of clear causal evidence and effective intervention means for what kind of host genetic factors drive the difference and what the key signal molecules are behind it.
[0003] As the most widely distributed staple crop in the world, corn shows significant yield advantage and genetic improvement potential. Corn inbred lines B73 and Mo17 are classic model materials, and there are differences in growth performance and stress resistance. Studies have shown that Mo17 usually shows better growth vigor than B73 in the seedling stage. At present, single or complex microbial inoculants are generally applied in agricultural production to promote the growth of corn in the seedling stage, and the colonization ability of crops is greatly affected by the original microbial community, so the effect is not stable. Another idea is to "tame" the rhizosphere microbiome by applying specific substances to make it succeed in a more beneficial direction. However, there is no report on such specific substances at present. SUMMARY
[0004] In view of the low growth vigor of corn in the seedling stage in the prior art, the application provides application of adenine in promoting growth of corn, which promotes growth of corn in the seedling stage by applying adenine to the root system of corn, is simple and stable in operation, and enriches beneficial growth-promoting bacteria by applying specific signal molecules adenine to directionally reshape the rhizosphere microbial community structure of corn, thereby significantly promoting the vegetative growth and biomass accumulation of corn plants.
[0005] The application is implemented by the following technical solutions: In the application, application of adenine in promoting growth of corn is provided, and the adenine promotes growth of corn in the seedling stage by recruiting beneficial microorganisms.
[0006] Further, the adenine solution or solid composition is applied to the rhizosphere of corn in the early growth stage of corn.
[0007] Further, the adenine aqueous solution is applied to the root system of corn.
[0008] Further, the concentration of the adenine aqueous solution is 2-10 mM.
[0009] Further, the adenine aqueous solution is administered once every 3 days, and is administered for 3 times continuously.
[0010] Further, the corn is B73 genotype corn.
[0011] In the present application, by exogenous application of adenine, plant rhizosphere beneficial microorganisms (such as Pseudomonas, Terriglobus, Shewanella, etc.) are specifically recruited and enriched, so as to improve the rhizosphere microbial community structure, promote the vegetative growth of corn and increase the biomass.
[0012] Compared with the prior art, the present application has the following beneficial effects: (1) Compared with the prior art, the present application first discovers and confirms that adenine is a key signal molecule for driving corn genotype-specific microbial assembly, and exogenous addition can directly and efficiently change the microbial community structure to a beneficial direction.
[0013] (2) Exogenous application of adenine can significantly promote the increase of root length, fresh weight, dry weight of corn seedlings, and increase the starch content, indicating that it optimizes the physiological state and carbon assimilation efficiency of plants.
[0014] (3) Adenine is a naturally occurring purine substance, which is environmentally friendly and different from chemical fertilizers and pesticides, and meets the development direction of green and sustainable agriculture.
[0015] (4) The present application reveals a new mechanism of plant-microbe interaction from the causal chain of “plant signal-microbe response-phenotype feedback”, which provides a new theoretical basis and technical path for crop microecological regulation. BRIEF DESCRIPTION OF DRAWINGS
[0016] Figure 1 B73 and Mo17 rhizosphere microbial community Alpha diversity comparison chart; (a) community evenness and richness, (b) concentration of dominant species in the community, (c) uniformity of species relative abundance; Figure 2 B73 and Mo17 rhizosphere microbial community Alpha diversity comparison chart; (a) community evenness and richness, (b) concentration of dominant species in the community, (c) uniformity of species relative abundance; Figure 3 B73 and Mo17 rhizosphere microbial community Alpha diversity comparison chart; (a) community evenness and richness, (b) concentration of dominant species in the community, (c) uniformity of species relative abundance; Figure 4 B73 and Mo17 rhizosphere microbial community Alpha diversity comparison chart; (a) community evenness and richness, (b) concentration of dominant species in the community, (c) uniformity of species relative abundance; Figure 5 B73 and Mo17 rhizosphere microbial community Alpha diversity comparison chart; (a) community evenness and richness, (b) concentration of dominant species in the community, (c) uniformity of species relative abundance; DETAILED DESCRIPTION
[0017] The application will be further described in conjunction with specific examples. It should be understood that these examples are only used to illustrate the application and not used to limit the scope of the application. The experimental methods in the following examples, if not otherwise specified, are generally carried out according to the conventional conditions or according to the conditions recommended by the manufacturers.
[0018] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The reagents or materials used in the application can be purchased through conventional routes. Unless otherwise specified, the reagents or materials used in the application are used according to the conventional methods in the art or according to the product instructions.
[0019] Example 1 The experiment first sets up three parallel plots with an area of 20 m², and the plot spacing is 1.5 meters. In each plot, the corn inbred line B73 and Mo17 are planted alternately in rows (B73, Mo17, B73 and Mo17 in turn, with a row spacing of about 60 cm). The comparison is realized in the same environment. At the key growth periods of 28 days and 42 days after sowing, soil samples attached to the roots of B73 and Mo17 corn are collected from each plot, and the Alpha diversity of the rhizosphere soil microbial community of B73 corn and Mo17 corn is analyzed. The comparison chart is shown in Figure 1 (a) represents the evenness and richness of the community, (b) represents the concentration of dominant species in the community, and (c) represents the uniformity of the relative abundance of species. It can be seen from Figure 1 that the community diversity indexes of B73 and Mo17 are significantly different (P<0.05); the Shannon index, Simpson index and Pielou evenness index in Mo17 are all higher than those in B73, indicating that Mo17 has greater root microbial diversity and more uniform community distribution. p
[0020] Example 2 B73 and Mo17 are planted under strict sterile water culture conditions, and corn seedlings are cultured with 1 / 2 Hoagland nutrient solution for 28 days to ensure stable nutrients and pH. Then the root exudates of B73 and Mo17 are collected by dark induction, and non-targeted metabolomics analysis is carried out. The principal component analysis result chart is shown in Figure 2 (a) represents the evenness and richness of the community, (b) represents the concentration of dominant species in the community, and (c) represents the uniformity of the relative abundance of species. It can be seen from Figure 2 It can be seen that the two genotypes B73 and Mo17 were separated along principal component 1 (PC1, explaining 20.95% of the variance) and PC2 (15.47% of the variance). The quality control samples (QC) were tightly clustered, confirming the reliability and stability of the detection technology. The rhizosphere secretions of B73 and Mo17 were significantly different in metabolic composition. Through redundancy analysis on the differential root secretions and microbial communities, it was found that adenine was the key secretion for constructing the microbial community structure of Mo17.
[0021] Example 3 Corn Mo17 and B73 were cultured in natural soil in a growth chamber (26°C, 16 hours light / 8 hours dark, 60% relative humidity); after two weeks of planting, water and adenine solution were irrigated into the planted soil. B73 was set up two groups of treatments: the control group was irrigated with an equal amount of water, and the treatment group was irrigated with 2 mM adenine aqueous solution (8 replicates), treated every 3 days (50 mL each time), a total of 3 times. One week after the last treatment (jointing stage), the soil and plant samples attached to the corn roots were collected.
[0022] (1) The growth indicators such as plant height, root length, fresh weight and dry weight of corn were measured, and the results are shown in Figure 3 It can be seen from Figure 3 that compared with water treatment, adenine treatment significantly improved various growth parameters of B73 seedlings, with an increase of 27% in root length, and an increase of 18% and 16% in fresh weight and dry weight, respectively, indicating that adenine can promote the growth of corn in the early stage.
[0023] (2) The contents of nutrients such as amino acids, total protein, soluble sugar and starch were determined, and the results are shown in Figure 4 It can be seen from Figure 4 that compared with water treatment, adenine treatment slightly increased the contents of total protein and soluble sugar in B73 seedlings, and significantly increased starch accumulation, indicating that it may promote plant growth by enhancing the plant's carbon fixation capacity.
[0024] (3) The microbial community composition in the rhizosphere soil of corn roots was determined: the microbial communities of corn roots treated with adenine were statistically analyzed, and PCoA principal coordinate analysis was performed, and the results are shown in Figure 5 It can be seen from Figure 5 that the microbial communities between the B73 water treatment group, the B73 adenine treatment group and the Mo17 water treatment group were obviously separated (PERMANOVA, P=0.004), indicating that adenine treatment significantly changed the composition of B73 rhizosphere bacterial community, and more growth-promoting microbial groups were enriched in the B73 adenine treatment group (these were also highly enriched in Mo17). Adenine treatment enhanced the carbon fixation capacity of B73 by recruiting such beneficial microorganisms, promoting the growth of B73.
Claims
1. Use of adenine for promoting the growth of corn, characterized in that, Adenine promotes corn seedling growth by recruiting beneficial microorganisms.
2. The use of adenine according to claim 1 for promoting the growth of corn, characterized in that, A solution or solid composition containing adenine is applied to the rhizosphere of corn in the early growth stage of corn.
3. The use of adenine according to claim 2 for promoting the growth of corn, characterized in that, An aqueous adenine solution is applied to the corn root system.
4. The use of adenine according to claim 3 for promoting the growth of corn, characterized in that, The concentration of the aqueous adenine solution is 2-10 mM.
5. The use of adenine according to claim 3 for promoting the growth of corn, characterized in that, The aqueous adenine solution is applied every 3 days, 10-50 mL each time, for 3 consecutive times.
6. The use of adenine according to claim 1 for promoting the growth of corn, characterized in that, The corn is of B73 genotype.