Rosa roxburghii pulp soluble prebiotic dietary fiber powder as well as preparation method and application thereof

Abstract

The invention discloses roxburgh rose pulp soluble prebiotic dietary fiber powder and a preparation method and application thereof.The preparation method includes the steps that roxburgh roses are juiced, pulp is subjected to microbial fermentation, a roxburgh rose fruit vinegar stock solution is added into the fermented pulp, uniform stirring is conducted, drying and smashing are conducted after ultrasonic treatment, and roxburgh rose pomace powder is obtained; after being treated by a compound enzyme enzymolysis process, the raw materials are precipitated by high-degree white spirit to obtain precipitates, namely the soluble prebiotic dietary fiber powder of the roxburgh rose pulp. According to the method disclosed by the invention, fibers of pulp of roxburgh rose after juicing are modified by utilizing biological fermentation, and are further extracted and enriched by adopting an acidic fruit vinegar stock solution and high-degree white spirit after enzymolysis, so that the method can be applied to prebiotic solid beverages rich in dietary fibers and various foods for improving intestinal health; the method solves the problem of comprehensive utilization of pulp after juicing of rosa roxburghii tratt, improves the added value of products, and can meet the requirements of consumers for intestinal health products.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of food processing, and particularly relates to a Rosa roxburghii fruit pulp soluble prebiotic dietary fiber powder and a preparation method and application thereof. BACKGROUND

[0002] Rosa roxburghii is difficult to eat directly due to its sour taste. The existing processing condition is mainly to further develop products after juicing. With the development of the Rosa roxburghii industry, the fruit pulp (pomace) after juicing has become a key factor limiting the sustainable development of the industry. According to the investigation, the yield of Rosa roxburghii fruit per mu is 1000 kg, and the yield of Rosa roxburghii fruit will reach 900,000 tons in 2025. At present, the utilization of Rosa roxburghii fruit is mainly juicing, and the juicing rate is about 50%. Therefore, 450,000 tons of Rosa roxburghii pomace by-products will be produced every year after 2025. The previous study shows that the basic nutritional components of Rosa roxburghii pomace are crude protein 7.07%, crude fiber 38.28%, crude fat 5.62%, and nitrogen-free extract 38.46%. In addition, the Rosa roxburghii pomace is also rich in active ingredients such as vitamins, plant polysaccharides, tannins, triterpenoid saponins, flavonoids, alkaloids, organic acids, and minerals; the research on the reuse of Rosa roxburghii pomace is mainly in the preparation of dietary fiber and feed additives, which is difficult to consume a large amount of Rosa roxburghii pomace. The traditional processing method of Rosa roxburghii pomace is mainly composting, which causes waste of resources and even pollution to the environment. Therefore, how to scaleably utilize the Rosa roxburghii pomace and realize high value-added and efficient utilization of the functional substances in the Rosa roxburghii pomace is the main technical difficulty in the utilization of the Rosa roxburghii pomace. Dietary fiber is known as the "seventh nutrient", and increasing intake helps to reduce the risk of chronic diseases such as diabetes, gastrointestinal diseases, and cardiovascular diseases. The physiological function of soluble dietary fiber (SDF) is usually more closely related to intestinal health. The content of soluble dietary fiber (SDF) in natural fiber is usually low. Therefore, how to efficiently extract or transform the soluble dietary fiber with higher content and better quality from the Rosa roxburghii pomace through suitable processing technology, and combine it with probiotics as prebiotics to improve its functions such as regulating intestinal flora, becomes an important direction of technical research and development. In order to facilitate application and improve experience, the Rosa roxburghii ingredients rich in dietary fiber are made into fruit powder and other solid forms, which is beneficial to retain the aroma and nutritional components of Rosa roxburghii, and facilitates transportation, storage and addition to other foods. SUMMARY

[0003] The present application aims to provide a Rosa roxburghii fruit pulp soluble prebiotic dietary fiber powder and a preparation method and application thereof, solve the comprehensive utilization problem of the fruit pulp after juicing, improve the product added value, and meet the needs of consumers for intestinal health products.

[0004] In order to achieve the above-mentioned target, the present application adopts the following technical solution: A method for preparing a Malus baccata fruit pulp soluble prebiotic dietary fiber powder, comprising the following steps: A. After fresh Malus baccata juice is extracted, the fruit pulp is prepared with water at a ratio of 1:4, inoculated with Lactobacillus acidophilus liquid seed culture medium at an inoculation amount of 3.5-5.5%, and cultured and fermented at a fermentation temperature of 37-39℃ for 36-48h. After fermentation, a fermentation liquor and Malus baccata fruit pulp fermentation residue are obtained; B. The fermentation residue obtained in step A is added with Malus baccata fruit vinegar stock solution, stirred uniformly, and then dried and crushed after ultrasonic treatment to obtain Malus baccata fruit pulp fermentation residue powder; the pH of the Malus baccata fruit vinegar stock solution is 2.5-3, and the addition amount of the Malus baccata fruit vinegar stock solution is 20-30% of the weight of the Malus baccata fruit pulp fermentation residue; the ultrasonic treatment conditions are an ultrasonic power of 400-600W and an ultrasonic time of 30-60min; and the drying process is drying in an oven at 45-55℃ for 36-48h; C. The Malus baccata fruit pulp fermentation residue powder obtained in step B is added with 2 times its weight of acetic acid-sodium acetate buffer solution, heated and kept at 38-45℃, then 0.01-0.03% of cellulase is added, and enzymolysis is carried out at 45-50℃ for 60-120min. After that, 4-6 times the weight of the Malus baccata fruit pulp fermentation residue powder of high-grade baijiu is added and precipitated overnight to obtain the Malus baccata fruit pulp soluble prebiotic dietary fiber powder. The alcohol content of the high-grade baijiu is 50-70%. The Malus baccata mentioned above is Malus baccata (Linn.) Borkh.

[0005] A Malus baccata fruit pulp soluble prebiotic dietary fiber powder is prepared by the above method.

[0006] A Malus baccata solid beverage rich in prebiotic soluble dietary fiber is prepared by mixing 70-80 parts of Malus baccata fruit pulp soluble prebiotic dietary fiber powder, 15-25 parts of auxiliary materials, and 5-15 parts of Malus baccata vitamin C freeze-dried powder.

[0007] The auxiliary materials are one or more of malt dextrin, β-cyclodextrin, whey protein, or sodium carboxymethyl cellulose.

[0008] The Malus baccata fruit pulp soluble prebiotic dietary fiber powder is used for preparing a prebiotic solid beverage rich in dietary fiber and various foods for improving intestinal health.

[0009] 1. The present application makes full use of a large amount of Malus baccata pomace produced after Malus baccata juice processing as the main raw material. This greatly improves the comprehensive utilization value of Malus baccata, converts the by-product that would have been discarded or only used as cheap feed into a high-value-added product, effectively reduces the discharge of solid waste, solves the environmental pressure faced by the Malus baccata processing industry, and conforms to the concept of green and sustainable development.

[0010] 2. The present application uses Lactobacillus acidophilus fermentation to improve dietary fiber, and further uses acid fruit vinegar stock and high-grade liquor to extract and enrich soluble dietary fiber. The process has the advantages of mild conditions, strong specificity and high efficiency, and avoids pollution and safety hazards that may be caused by strong acid and strong alkali chemical methods.

[0011] 3. The prepared soluble dietary fiber can be used as a prebiotic and has health attributes such as regulating intestinal flora compared with insoluble dietary fiber.

[0012] 4. The entire process uses food-grade raw materials, and the obtained product can be applied to the development of food, functional food and health food. At the same time, the use of ultrasonic extraction greatly improves the dissolution of dietary fiber. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 Picture of untreated Malus halliana pomace Figure 2 Picture of Malus halliana flesh soluble prebiotic dietary fiber powder DETAILED DESCRIPTION

[0014] Example 1: Preparation process parameter 1 A. After fresh Malus halliana juice is extracted, the flesh is prepared with water at a ratio of 1:4, inoculated with Lactobacillus acidophilus liquid seed medium, the inoculation amount is 3.5%, the fermentation temperature is 39℃, and the fermentation is cultured for 36h. After the fermentation is completed, fermentation broth and Malus halliana flesh fermentation residue are obtained; B. The fermentation residue obtained in step A is added with Malus halliana fruit vinegar stock, stirred uniformly and treated with ultrasonic waves, then dried, crushed to obtain Malus halliana flesh fermentation residue powder; the pH of the Malus halliana fruit vinegar stock is 3, the addition amount of the Malus halliana fruit vinegar stock is 20% of the weight of the Malus halliana flesh fermentation residue; the ultrasonic treatment conditions are ultrasonic power of 600W and ultrasonic time of 30min; the drying process is drying in a 55℃ oven for 36h; C. The Malus halliana flesh fermentation residue powder obtained in step B is added with 2 times its weight of acetic acid-sodium acetate buffer, heated and kept at 45℃, then 0.01% of cellulase is added, and the mixture is enzymatically hydrolyzed at 50℃ for 60min. Then 6 times the weight of the Malus halliana flesh fermentation residue powder of high-grade liquor is added and left overnight to precipitate, and the obtained precipitate is Malus halliana flesh soluble prebiotic dietary fiber powder. The alcohol content of the high-grade liquor is 50%; The above-mentioned Malus halliana is Malus halliana.

[0015] A Malus halliana flesh soluble prebiotic dietary fiber powder is prepared by the above method.

[0016] A solid drink of roxburgh rose rich in soluble dietary fiber of prebiotics, which is prepared by mixing 80 parts of roxburgh rose pulp soluble prebiotic dietary fiber powder, 15 parts of auxiliary materials and 5 parts of roxburgh rose vitamin C freeze-dried powder.

[0017] The auxiliary material is one or a mixture of more than one of malt dextrin, beta-cyclodextrin, whey protein or sodium carboxymethyl cellulose.

[0018] The roxburgh rose pulp soluble prebiotic dietary fiber powder is used for preparing a solid drink of prebiotics rich in dietary fiber and various foods for improving intestinal health.

[0019] Example 2: preparation process parameters 2 A. After fresh roxburgh rose juice is extracted, the pulp is prepared with water at a ratio of 1:4, inoculated with Lactobacillus acidophilus liquid seed culture medium, inoculated at 5.5%, fermented at 37℃ for 48h, and then fermented to obtain fermented liquid and roxburgh rose pulp fermentation residue; B. The fermentation residue obtained in step A is added with roxburgh rose fruit vinegar stock solution, stirred uniformly and treated with ultrasonic waves, then dried, crushed to obtain roxburgh rose pulp fermentation residue powder; the pH of the roxburgh rose fruit vinegar stock solution is 2.5, the addition amount of the roxburgh rose fruit vinegar stock solution is 30% of the weight of the roxburgh rose pulp fermentation residue; the ultrasonic treatment conditions are ultrasonic power 400W and ultrasonic time 60min; the drying process is drying in a 45℃ oven for 48h; C. The roxburgh rose pulp fermentation residue powder obtained in step B is added with 2 times its weight of acetic acid-sodium acetate buffer, heated and kept at 38℃, then 0.03% of cellulase is added, heated to 45℃ for enzymolysis for 120min, then 4 times the mass of the roxburgh rose pulp fermentation residue powder of high-grade baijiu is added and precipitated overnight, and the precipitate obtained is the roxburgh rose pulp soluble prebiotic dietary fiber powder. The alcohol content of the high-grade baijiu is 70%; The roxburgh rose mentioned above is a gold roxburgh rose.

[0020] A roxburgh rose pulp soluble prebiotic dietary fiber powder is prepared by the above method.

[0021] A solid drink of roxburgh rose rich in soluble dietary fiber of prebiotics, which is prepared by mixing 80 parts of roxburgh rose pulp soluble prebiotic dietary fiber powder, 15 parts of auxiliary materials and 5 parts of roxburgh rose vitamin C freeze-dried powder.

[0022] The auxiliary material is one or a mixture of more than one of malt dextrin, beta-cyclodextrin, whey protein or sodium carboxymethyl cellulose.

[0023] The application of the Malus hallings fruit pulp soluble prebiotic dietary fiber powder in the preparation of prebiotic solid beverages rich in dietary fiber and various foods for improving intestinal health.

[0024] Example 3: preparation process parameter 3 A. After fresh Malus hallings juice extraction, the fruit pulp was prepared with water at a ratio of 1:4, inoculated with Lactobacillus acidophilus liquid seed culture medium, inoculation amount 4.0%, fermentation temperature 38℃, and cultured for 40h. After fermentation, fermentation broth and Malus hallings fruit pulp fermentation residue were obtained; B. The fermentation residue obtained in step A was added with Malus hallings fruit vinegar stock solution, stirred uniformly and treated with ultrasonic waves, then dried, crushed to obtain Malus hallings fruit pulp fermentation residue powder; the pH of the Malus hallings fruit vinegar stock solution was 2.8, and the addition amount of the Malus hallings fruit vinegar stock solution was 25% of the weight of the Malus hallings fruit pulp fermentation residue; the ultrasonic treatment conditions were ultrasonic power 500W and ultrasonic time 45min; the drying process was drying in a 50℃ oven for 45h; C. The Malus hallings fruit pulp fermentation residue powder obtained in step B was added with 2 times its weight of acetic acid-sodium acetate buffer, heated and kept at 40℃, then 0.02% cellulase was added, and the mixture was enzymatically hydrolyzed at 48℃ for 100min, then 5 times the weight of the Malus hallings fruit pulp fermentation residue powder of high-grade baijiu was added and precipitated overnight, and the precipitate obtained was the Malus hallings fruit pulp soluble prebiotic dietary fiber powder. The alcohol content of the high-grade baijiu was 60%; The Malus hallings mentioned above is Malus hallings.

[0025] A Malus hallings fruit pulp soluble prebiotic dietary fiber powder is prepared by the above method.

[0026] A Malus hallings solid beverage rich in prebiotic soluble dietary fiber is prepared by mixing 75 parts of Malus hallings fruit pulp soluble prebiotic dietary fiber powder, 20 parts of auxiliary materials, and 5 parts of Malus hallings vitamin C freeze-dried powder.

[0027] The auxiliary materials are one or more of malt dextrin, beta-cyclodextrin, whey protein, or sodium carboxymethyl cellulose.

[0028] The application of the Malus hallings fruit pulp soluble prebiotic dietary fiber powder in the preparation of prebiotic solid beverages rich in dietary fiber and various foods for improving intestinal health.

[0029] The above merely describes preferred embodiments of the present application, but is not intended to limit the present application to other forms, and any person skilled in the art can make changes or modifications to the above disclosed technical contents into equivalent embodiments with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiments without departing from the technical solution content of the present application, and according to the technical essence of the present application, still belongs to the protection scope of the technical solution of the present application.

Claims

1. A method for preparing a soluble prebiotic dietary fiber powder from prickly pear pulp, characterized in that, Includes the following steps: A. Juice fresh prickly pear and mix the pulp with water at a ratio of 1:

4. Inoculate with Lactobacillus acidophilus liquid seed culture medium at an inoculation amount of 3.5-5.5%. Ferment at 37-39℃ for 36-48 hours. After fermentation, fermentation liquid and prickly pear pulp fermentation residue are obtained. B. Add the fermentation residue obtained in step A to the original solution of prickly pear fruit vinegar, stir evenly, and then dry and crush it after ultrasonic treatment to obtain prickly pear pulp fermentation residue powder. C. Add 2 times its weight of the prickly pear pulp fermented residue powder to acetic acid-sodium acetate buffer solution, heat and keep warm at 38-45℃, then add 0.01-0.03% of its weight of cellulase, heat to 45-50℃ for enzymatic hydrolysis for 60-120 minutes, then add 4-6 times the weight of the prickly pear pulp fermented residue powder of high-proof liquor and let it precipitate overnight. The precipitate obtained is the prickly pear pulp soluble prebiotic dietary fiber powder.

2. The method for preparing a soluble prebiotic dietary fiber powder from prickly pear pulp according to claim 1, characterized in that, The pH of the prickly pear fruit vinegar stock solution in step B is 2.5-3, and the amount of prickly pear fruit vinegar stock solution added is 20-30% of the weight of the prickly pear pulp fermentation residue; the conditions for ultrasonic treatment are ultrasonic power of 400-600W and ultrasonic time of 30-60min; the drying process is drying in an oven at 45-55℃ for 36-48h.

3. The method for preparing a soluble prebiotic dietary fiber powder from prickly pear pulp according to claim 1, characterized in that, The alcohol content of the high-proof liquor in step C is 50-70%.

4. The method for preparing a soluble prebiotic dietary fiber powder from prickly pear pulp according to claim 1, characterized in that, The prickly pear mentioned is the golden prickly pear.

5. A soluble prebiotic dietary fiber powder made from prickly pear pulp, characterized in that, It was prepared using a method described in any one of claims 1-4 for preparing prebiotic dietary fiber powder by juicing prickly pear and preparing the pulp.

6. A prickly pear solid beverage rich in prebiotic soluble dietary fiber, characterized in that, Based on the weight percentages, a prickly pear solid beverage rich in prebiotic soluble dietary fiber is prepared by compounding and mixing 70-80 parts of the prickly pear pulp soluble prebiotic dietary fiber powder as described in claim 5, 15-25 parts of excipients, and 5-15 parts of prickly pear vitamin C freeze-dried powder.

7. A prickly pear solid beverage rich in prebiotic soluble dietary fiber according to claim 6, characterized in that, The excipient is one or more of maltodextrin, β-cyclodextrin, whey protein, or sodium carboxymethyl cellulose.

8. The application of the prickly pear pulp soluble prebiotic dietary fiber powder according to claim 5 in the preparation of prebiotic solid beverages rich in dietary fiber and various foods that improve intestinal health.

Images