Vitamin E liposome-containing composition and method for preparing tobacco filtering product by using vitamin E liposome-containing composition

Abstract

The invention belongs to the technical field of cigarette harm reduction and functionalization, and particularly relates to a vitamin E lipidosome-containing composition with slow-release anti-oxidation and sensory improvement functions and a method for preparing a tobacco filter product by using the vitamin E lipidosome-containing composition. Comprising an oil-phase composition and a water-phase composition, the oil-phase composition comprises vitamin E acetate, soya bean lecithin and beta-sitosterol, the water-phase composition comprises a stabilizer, and the weight ratio of the oil-phase composition to the water-phase composition is 3: 4. The lipidosome powder is added into the filter tip through an alcohol-free formula and an emulsification technology, so that the original function of the filter tip is maintained, free radical damage in smoke flow is effectively delayed, and the smoking smoothness is improved. The process has the characteristics of simplicity and convenience in operation, environment friendliness and low toxicity, and the obtained functional filter tip is suitable for industrial production.

Description

Technical Field

[0001] This invention belongs to the field of cigarette harm reduction and functionalization technology, specifically relating to a composition containing vitamin E liposomes that has both slow-release antioxidant and sensory enhancement functions, and a method for preparing tobacco filter products thereon. Background Technology

[0002] Currently, the tobacco industry is actively promoting the research and development of harm reduction technologies and functional filters, especially in key areas such as antioxidant protection, free radical scavenging, and sensory experience optimization. Vitamin E (α-tocopherol), as a potent fat-soluble antioxidant, possesses a unique molecular structure that endows it with three core values: 1) efficiently scavenging free radicals in cigarette smoke and reducing oxidative stress damage; 2) protecting the inherent aroma components of tobacco from oxidative degradation; and 3) improving the smoothness of smoking through lubrication. However, directly applying vitamin E to filters faces significant technical challenges: firstly, vitamin E is easily oxidized and deactivated during processing and storage; secondly, traditional addition methods exhibit a burst-release effect, failing to achieve sustained release; and thirdly, fat-soluble components are difficult to disperse uniformly in hydrophilic filter materials. Summary of the Invention

[0003] To address the problems existing in the prior art, this invention provides a composition containing vitamin E liposomes and a method for preparing tobacco filter products thereon. This solves the technical problems of easy oxidation and difficulty in sustained release of vitamin E in filter applications, significantly improving the stability of liposomes under high-temperature processing conditions and achieving sustained release.

[0004] The technical problem to be solved by the present invention is achieved through the following technical solution: A composition containing vitamin E liposomes, comprising an oil phase composition and an aqueous phase composition, wherein the oil phase composition comprises vitamin E acetate, soybean lecithin, and β-sitosterol, and the aqueous phase composition comprises a stabilizer, wherein the weight ratio of the oil phase composition to the aqueous phase composition is 3:4 by weight percentage.

[0005] In the above-mentioned composition containing vitamin E liposomes, the weight ratio of vitamin E acetate, soybean lecithin, and β-sitosterol is 20~35:10~15:8 by weight percentage.

[0006] In the above-mentioned composition containing vitamin E liposomes, the stabilizer accounts for 20% of the aqueous phase composition by weight percentage.

[0007] The stabilizer in the above-mentioned composition containing vitamin E liposomes is trehalose.

[0008] A method for preparing tobacco filter articles from a composition containing vitamin E liposomes, comprising the following steps: (1) Calculate by weight percentage, take 20-35 parts of vitamin E acetate, 10-15 parts of soybean lecithin and 8 parts of β-sitosterol and dissolve them in 100 parts of organic solvent to form an oil phase composition; (2) Calculate by weight percentage, take 20 parts of stabilizer and dissolve it in 100 parts of pH 7.4 phosphate buffer to form an aqueous phase composition; (3) Take 20 parts of the aqueous phase composition and add 15-20 parts of the oil phase composition, homogenize at high speed to form a W / O type primary emulsion; prepare a liposome suspension with uniform particle size using a homogenization process; (4) Take 25 parts of colostrum and pour it into 20 parts of aqueous phase containing 1% Tween 80. Homogenize twice to obtain W / O / W type double emulsion. Remove organic solvent by rotary evaporation, homogenize again at high speed, and use low temperature spray drying process to obtain flowable liposome powder. Obtain good flowable liposome powder by trehalose protection and spray drying parameter optimization. (5) The liposome powder and cellulose acetate slurry are mixed at a mass percentage ratio of 1:100 to 5:100 and then spun into filter rods, with the liposomes uniformly embedded in the fiber pores. The nanoliposome interface is modified with β-sitosterol to form a stable composite structure with the cellulose acetate matrix.

[0009] The method for preparing tobacco filter products using the above-mentioned composition containing vitamin E liposomes, wherein the organic solvent is ethyl acetate.

[0010] The method for preparing tobacco filter products using the above-mentioned composition containing vitamin E liposomes, wherein the stabilizer is trehalose.

[0011] The beneficial effects of this invention are as follows: 1. By using liposome encapsulation technology and β-sitosterol interface modification, the problem of easy oxidation and degradation in traditional addition methods has been solved.

[0012] 2. The innovative W / O / W three-level drug delivery structure enables precise sustained release, ensuring uniform release of vitamin E during aspiration, avoiding burst release effects, and guaranteeing antioxidant effects throughout the process. Detailed Implementation

[0013] The following embodiments are provided to better understand the present invention and are not limited to the preferred embodiments described. They do not constitute a limitation on the content and scope of protection of the present invention. Any product that is the same as or similar to the present invention, derived by any person under the guidance of the present invention or by combining the features of the present invention with other prior art, falls within the protection scope of the present invention.

[0014] Where specific experimental steps or conditions are not specified in the embodiments, they can be performed according to the conventional experimental steps or conditions described in the literature in this field. All raw materials or instruments used are commercially available conventional products, including but not limited to those used in the embodiments of this application.

[0015] Example 1 This embodiment provides a composition of vitamin E-containing liposomes with an alcohol-free stabilized system, comprising an oil phase composition and an aqueous phase composition, wherein the weight ratio of the oil phase composition to the aqueous phase composition is 3:4. The oil phase composition comprises vitamin E acetate, soybean lecithin, and β-sitosterol, with 35 parts by weight of vitamin E acetate, 15 parts by weight of soybean lecithin, and 8 parts by weight of β-sitosterol; the aqueous phase composition comprises a stabilizer, namely 20 parts by weight of trehalose, wherein the stabilizer accounts for 20% of the aqueous phase composition.

[0016] The method for preparing tobacco filter products using the composition containing vitamin E liposomes in this embodiment is as follows: (1) Weigh 35 parts of vitamin E acetate, 15 parts of soybean lecithin and 8 parts of β-sitosterol by weight and dissolve them in 100 parts of organic solvent ethyl acetate to form an oil phase composition; (2) Calculate by weight, take 20 parts of trehalose and dissolve them in 100 parts of pH 7.4 phosphate buffer to form an aqueous phase composition; (3) Take 20 parts of the aqueous phase composition and add 15-20 parts of the oil phase composition, then homogenize at high speed to form a W / O type primary emulsion; (4) Take 25 parts of colostrum and pour it into 20 parts of the aqueous phase composition described in step (2) containing 1% Tween 80. Homogenize twice to obtain W / O / W type double emulsion. Remove organic solvent by rotary evaporation, homogenize again at high speed, and use low temperature spray drying process to obtain free-flowing liposome powder. (5) The prepared liposome powder is mixed with cellulose acetate slurry at a ratio of 1:100 according to the weight parts, and then spun into a filter rod, in which the liposomes are uniformly embedded in the fiber pores.

[0017] Example 2 This embodiment provides an alcohol-free stabilized composition containing vitamin E liposomes, comprising an oil phase composition and an aqueous phase composition, with a weight ratio of 3.5:4. The oil phase composition comprises vitamin E acetate, soybean lecithin, and β-sitosterol, in parts by weight: 20 parts vitamin E acetate, 10 parts soybean lecithin, and 8 parts β-sitosterol. The aqueous phase composition comprises a stabilizer, namely 20 parts trehalose, with the stabilizer accounting for 20% of the aqueous phase composition.

[0018] The method for preparing tobacco filter products using the composition containing vitamin E liposomes in this embodiment is as follows: (1) Weigh 20 parts of vitamin E acetate, 10 parts of soybean lecithin and 8 parts of β-sitosterol by weight and dissolve them in 100 parts of organic solvent ethyl acetate to form an oil phase composition; (2) Calculate by weight, take 20 parts of trehalose and dissolve them in 100 parts of pH 7.4 phosphate buffer to form an aqueous phase composition; (3) Take 20 parts of the aqueous phase composition and add 17.5 parts of the oil phase composition, and homogenize at high speed to form a W / O type primary emulsion; (4) Take 25 parts of colostrum and pour it into 20 parts of the aqueous phase composition described in step (2) containing 1% Tween 80. Homogenize twice to obtain W / O / W type double emulsion. Remove organic solvent by rotary evaporation, homogenize again at high speed, and use low temperature spray drying process to obtain free-flowing liposome powder. (5) The prepared liposome powder and cellulose acetate slurry are mixed at a ratio of 5:100 according to the weight ratio, and then spun into filter rods. The liposomes are uniformly embedded in the fiber pores.

[0019] Example 3 This embodiment provides an alcohol-free stabilized composition containing vitamin E liposomes, comprising an oil phase composition and an aqueous phase composition, with a weight ratio of 1:1. The oil phase composition comprises vitamin E acetate, soybean lecithin, and β-sitosterol, with 28 parts by weight of vitamin E acetate, 12 parts of soybean lecithin, and 8 parts of β-sitosterol; the aqueous phase composition comprises a stabilizer, namely 20 parts of trehalose, with the stabilizer accounting for 20% of the aqueous phase composition.

[0020] The method for preparing tobacco filter products using the composition containing vitamin E liposomes in this embodiment is as follows: (1) Weigh 28 parts of vitamin E acetate, 12 parts of soybean lecithin and 8 parts of β-sitosterol by weight and dissolve them in 100 parts of organic solvent ethyl acetate to form an oil phase composition; (2) Calculate by weight, take 20 parts of trehalose and dissolve them in 100 parts of pH 7.4 phosphate buffer to form an aqueous phase composition; (3) Take 20 parts of the aqueous phase composition and add 20 parts of the oil phase composition, then homogenize at high speed to form a W / O type primary emulsion; (4) Take 25 parts of colostrum and pour it into 20 parts of the aqueous phase composition described in step (2) containing 1% Tween 80. Homogenize twice to obtain W / O / W type double emulsion. Remove organic solvent by rotary evaporation, homogenize again at high speed, and use low temperature spray drying process to obtain free-flowing liposome powder. (5) The prepared liposome powder and cellulose acetate slurry are mixed at a ratio of 5:100 according to the weight ratio, and then spun into filter rods. The liposomes are uniformly embedded in the fiber pores.

[0021] Comparative Example 1 This comparative example is an alcohol-free stable system. The difference from Example 1 is that the weight parts of each component in the oil phase composition are: 40 parts of vitamin E acetate, 15 parts of soybean lecithin, and 8 parts of β-sitosterol. Other components and preparation methods are the same as in Example 1.

[0022] Comparative Example 2 This comparative example is an alcohol-free stable system. The difference from Example 1 is that the weight parts of each component in the oil phase composition are: 15 parts of vitamin E acetate, 15 parts of soybean lecithin, and 8 parts of β-sitosterol. Other components and preparation methods are the same as in Example 1.

[0023] Comparative Example 3 This comparative example is an alcohol-free stable system. The difference from Example 2 is that the weight parts of each component in the oil phase composition are: 20 parts of vitamin E acetate, 20 parts of soybean lecithin, and 8 parts of β-sitosterol. Other components and preparation methods are the same as in Example 2.

[0024] Comparative Example 4 This comparative example is an alcohol-free stable system. The difference from Example 2 is that the weight parts of each component in the oil phase composition are: 20 parts of vitamin E acetate, 5 parts of soybean lecithin, and 8 parts of β-sitosterol. Other components and preparation methods are the same as in Example 2.

[0025] Comparative Example 5 This comparative example is an alcohol-free stable system. The difference from Example 1 is that the weight parts of each component in the oil phase composition are: 15 parts of vitamin E acetate, 15 parts of soybean lecithin, and 13 parts of β-sitosterol. Other components and preparation methods are the same as in Example 1.

[0026] Comparative Example 6 This comparative example is an alcohol-free stable system. The difference from Example 1 is that the weight parts of each component in the oil phase composition are: 15 parts of vitamin E acetate, 15 parts of soybean lecithin, and 3 parts of β-sitosterol. Other components and preparation methods are the same as in Example 1.

[0027] Sensory evaluation 1 A panel of ten experts with national cigarette tasting qualifications evaluated the cigarettes prepared in Examples 1-3 and Comparative Examples 1-6. The evaluations were conducted according to GB5606.4-2005 "Cigarettes Part 4: Sensory Technical Requirements" regarding the cigarettes' gloss, aroma, harmony, off-flavors, irritation, and aftertaste. The average values ​​of the evaluations were recorded, and the total sensory quality score was calculated.

[0028] The sensory evaluation results are shown in Table 1 below. Table 1 Sensory evaluation results

[0029] According to the data comparison of Examples 1-3 and Comparative Examples 1-6, both Examples 1-3 and Comparative Examples 1-6 used vitamin E acetate, soybean lecithin, β-sitosterol and ethyl acetate in a synergistic formulation to prepare an alcohol-free stable system. Comparative Examples 1-2 changed the vitamin E acetate content compared to Example 1; Comparative Examples 3-4 adjusted the soybean lecithin content compared to Example 2; Comparative Examples 5-6 changed the β-sitosterol content compared to Example 3. The sensory scores of Examples 1-3 were better than those of Comparative Examples 1-6 to some extent. This indicates that by using vitamin E acetate, soybean lecithin, β-sitosterol and ethyl acetate in a synergistic formulation, and with the weight ratio of vitamin E acetate, soybean lecithin and β-sitosterol in the range of 20~35:10~15:8, it is beneficial to achieve a synergistic effect between sensory performance and harm reduction function, providing a quantifiable and easily industrialized solution for the development of high-end tobacco products.

[0030] This invention utilizes soybean lecithin and β-sitosterol to construct an alcohol-free, stable system, avoiding the hepatotoxicity and nephrotoxicity risks associated with traditional liposomes. Through a dual emulsification-homogenization technique, a W / O / W three-level drug-loading structure is developed to achieve gradient release during aspiration and precise particle size control. Trehalose is innovatively introduced as a cryoprotectant, ensuring the structural integrity of the spray-dried liposome powder. A filter-compatible loading process using a blended spinning method (cellulose acetate matrix drug loading) is established, precisely controlling the drug loading at 0.5-2.0 mg / cm³. This invention not only solves the stability and controlled-release challenges of vitamin E in tobacco applications but also pioneers a new paradigm for "nano-harm reduction" filters.

Claims

1. A composition containing vitamin E liposomes, characterized in that, The composition comprises an oil phase composition and an aqueous phase composition, wherein the oil phase composition comprises vitamin E acetate, soybean lecithin, β-sitosterol and an organic solvent, and the aqueous phase composition comprises a stabilizer, and the weight ratio of the oil phase composition to the aqueous phase composition is 3 to 4:4 by weight.

2. The composition containing vitamin E liposomes according to claim 1, characterized in that, The weight ratio of vitamin E acetate, soy lecithin, and β-sitosterol is 20~35:10~15:8, calculated by weight parts.

3. The composition containing vitamin E liposomes according to claim 1, characterized in that, The stabilizer accounts for 20% of the aqueous phase composition by weight.

4. The composition containing vitamin E liposomes according to claim 1, characterized in that, The organic solvent is ethyl acetate.

5. The composition containing vitamin E liposomes according to claim 1, characterized in that, The stabilizer is trehalose.

6. A method for preparing tobacco filter articles from the composition containing vitamin E liposomes according to claims 1-4, characterized in that, Includes the following steps: (1) Calculate by weight parts, take 20-35 parts of vitamin E acetate, 10-15 parts of soybean lecithin and 8 parts of β-sitosterol and dissolve them in 100 parts of organic solvent to form an oil phase composition; (2) Calculate by weight, take 20 parts of stabilizer and dissolve it in 100 parts of pH 7.4 phosphate buffer to form an aqueous phase composition; (3) Take 20 parts of the aqueous phase composition and add 15-20 parts of the oil phase composition, then homogenize at high speed to form a W / O type primary emulsion; (4) Take 25 parts of colostrum and pour it into 20 parts of the aqueous phase of claim 5-(2) containing 1% Tween 80. Homogenize twice to obtain W / O / W type double emulsion. Remove organic solvent by rotary evaporation, homogenize again at high speed, and use low temperature spray drying process to obtain free-flowing liposome powder. (5) The liposome powder and cellulose acetate slurry are mixed at a ratio of 1:100 to 5:100 by weight and spun into a filter rod, with the liposomes uniformly embedded in the fiber pores.

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