Composition for promoting growth and development of children and application thereof

Abstract

The invention relates to a composition for promoting growth and development of children and application thereof, and belongs to the technical field of traditional Chinese medicines. The composition consists of the following traditional Chinese medicine extracts in parts by mass: 4-10 parts of a traditional Chinese medicine extract A, 3-7 parts of a traditional Chinese medicine extract B, 1-3 parts of a traditional Chinese medicine extract C and 2-6 parts of a traditional Chinese medicine extract D, on the basis of an ancient and classical prescription, codonopsis pilosula, Chinese yam, white hyacinth bean, poria cocos, platycodon grandiflorum and semen coicis are taken as main medicinal materials, effective components of different medicinal materials are extracted, separated and purified through a scientific and technological method and an instrument, reasonable proportioning is performed, the curative effect is exerted, and through pharmacodynamic research, the effect is remarkable. Therefore, the traditional Chinese medicine composition can improve the production and development of children by enhancing the absorption of the spleen and the stomach. And the related process is advanced, and the dose is not large.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine, in particular to a composition for promoting growth and development of children and application thereof. BACKGROUND

[0002] The ancient classic prescription Shenling Baizhu Powder is used for tonifying the spleen and stomach, benefiting the lung qi, and is used for treating deficiency of the spleen and stomach, poor appetite, loose stool, shortness of breath, cough and lassitude. Shenling Baizhu Powder is composed of Radix Sophorae Tonkinensis, Rhizoma Atractylodis Macrocephalae, Tuckahoe, Radix Glycyrrhizae, Platycodonis, Nelumbinis Plumula, Radix Ginseng, Fructus Amomi Rotundus, Rhizoma Dioscoreae and Rhizoma Coicis. Radix Ginseng is used for tonifying the spleen and stomach, Rhizoma Atractylodis Macrocephalae and Tuckahoe are used for drying dampness and tonifying the spleen, Rhizoma Dioscoreae, Rhizoma Coicis and Radix Sophorae Tonkinensis are used for tonifying the spleen and drying dampness, Fructus Amomi Rotundus is used for aromatizing dampness and descending adverse qi, Platycodonis is used for promoting the lung, and Radix Glycyrrhizae is used for harmonizing all the medicines. The combination of all the medicines can tonify the spleen and benefit the qi, and can also dry dampness and stop diarrhea.

[0003] However, the above-mentioned prescription has the following shortcomings: the process is relatively backward, and the dosage is large. SUMMARY

[0004] The present application aims at solving the technical problems of the prior art Shenling Baizhu Powder, which has a relatively backward process and a large dosage, and provides a composition for promoting growth and development of children and application thereof. The composition of the present application is based on the ancient classic prescription Shenling Baizhu Powder, and uses Radix Codonopsis, Rhizoma Dioscoreae, Radix Sophorae Tonkinensis, Tuckahoe, Platycodonis and Rhizoma Coicis as main medicinal materials. The effective components of different medicinal materials are extracted, separated and purified by scientific and technological methods and instruments, and are reasonably proportioned to exert the curative effect. The pharmacodynamic study shows that the traditional Chinese medicine composition of the present application can enhance the absorption of the spleen and stomach, thereby improving the growth and development of children. Moreover, the process is advanced, and the dosage is not large.

[0005] In order to solve the above-mentioned technical problems, the technical scheme of the present application is as follows: A composition for promoting growth and development of children is composed of the following mass parts of traditional Chinese medicine extracts: 4-10 parts of traditional Chinese medicine extract A, 3-7 parts of traditional Chinese medicine extract B, 1-3 parts of traditional Chinese medicine extract C and 2-6 parts of traditional Chinese medicine extract D; The preparation method of the traditional Chinese medicine extract A comprises the following steps: Radix Codonopsis, Rhizoma Dioscoreae, Rhizoma Coicis and Radix Sophorae Tonkinensis are taken, pectinase and water are added, microwave extraction is adopted, the extraction liquid is collected, and freeze-drying is performed to obtain the traditional Chinese medicine extract A; The preparation method of the traditional Chinese medicine extract B comprises the following steps: Radix Codonopsis is taken, citric acid solution is added to keep the temperature at 30-35℃, after soaking, the flow rate is controlled, the percolate is collected, and freeze-drying is performed to obtain the traditional Chinese medicine extract B; The preparation method of the traditional Chinese medicine extract C comprises the following steps: Tuckahoe and Platycodonis are taken, water extraction is performed for multiple times, the extraction liquids are combined, filtration is performed, concentration is performed, and freeze-drying is performed to obtain the traditional Chinese medicine extract C; The preparation method of the traditional Chinese medicine extract D comprises the following steps: The white hyacinth bean, the radix codonopsis and the Chinese yam are taken and put into a refrigerated superfine powder grinder, the temperature is controlled to be 15-25 DEG C, the powder is crushed, the crushed powder is collected, and the traditional Chinese medicine extract D is obtained.

[0006] Preferably, the composition of the present application is composed of the following mass parts of traditional Chinese medicine extracts: The traditional Chinese medicine extract A is 10 parts, the traditional Chinese medicine extract B is 3 parts, the traditional Chinese medicine extract C is 3 parts, and the traditional Chinese medicine extract D is 4 parts.

[0007] Preferably, in the preparation method of the traditional Chinese medicine extract A, the mass part ratio of the radix codonopsis, the Chinese yam, the coix seed and the white hyacinth bean is 8:7:4:4.

[0008] Preferably, in the preparation method of the traditional Chinese medicine extract A, the power of the microwave extraction is 320 w, and the microwave extraction time is 60 min.

[0009] Preferably, in the preparation method of the traditional Chinese medicine extract B, the mass part of the radix codonopsis is 10 parts.

[0010] Preferably, in the preparation method of the traditional Chinese medicine extract B, the temperature is kept to be 30 DEG C, the soaking time is 4 h, and the flow is controlled to be 12 h.

[0011] Preferably, in the preparation method of the traditional Chinese medicine extract C, the mass part ratio of the poria cocos and the platycladus orientalis is 12:4, and the water extraction times is 2 times, and each time is 1.5 hours.

[0012] Preferably, in the preparation method of the traditional Chinese medicine extract D, the mass part ratio of the white hyacinth bean, the radix codonopsis and the Chinese yam is 8:4:4, the temperature is controlled to be 20 DEG C, and the crushing is to 500 meshes.

[0013] The application of a kind of composition of the present application in preparing the medicine for promoting the growth and development of children.

[0014] The present application has the following beneficial effects: The composition for promoting children's growth and development of this invention comprises the following parts by weight of traditional Chinese medicine extracts: Traditional Chinese medicine extract A 4-10 parts, Traditional Chinese medicine extract B 3-7 parts, Traditional Chinese medicine extract C 1-3 parts, and Traditional Chinese medicine extract D 2-6 parts. It is obtained by extracting, separating, and purifying the effective components of different herbs using scientific and technological methods and instruments. Traditional Chinese medicine extract A is the polysaccharide portion of Codonopsis pilosula, Dioscorea opposita, Coix lacryma-jobi, and Dolichos lablab; Traditional Chinese medicine extract B is the alkaloid portion of Codonopsis pilosula; Traditional Chinese medicine extract C is the organic acid portion of Poria cocos and Platycodon grandiflorus; and Traditional Chinese medicine extract D is the ultrafine powder portion of Dolichos lablab, Codonopsis pilosula, and Dioscorea opposita. Through reasonable pharmacodynamic experiments, different extracts are combined in optimal proportions to enhance the absorption and efficacy of the active ingredients. Pharmacodynamic experiments have verified that the obtained traditional Chinese medicine composition can effectively increase the weight gain of mice, promote skeletal muscle growth and development, promote α-amylase activity, facilitate starch hydrolysis, promote nutrient absorption, promote the development and maturation of immune organs and the liver, promote immune function, and enhance immunity. The traditional Chinese medicine composition of the present invention can improve children's growth and development by enhancing the absorption of the spleen and stomach, and the process involved is advanced and the dosage is small. Detailed Implementation

[0015] The inventive concept of this invention is as follows: Based on the ancient classic formula Shenling Baizhu Powder, this invention uses Codonopsis pilosula, Dioscorea opposita, Dolichos lablab, Poria cocos, Platycodon grandiflorus, and Coix lacryma-jobi as the main medicinal materials. Different components are extracted, separated, enriched, and purified using different methods to obtain extracts of different components. The extracts are then rationally proportioned, formulated into a preparation, and through animal experiments, a composition that promotes children's growth and development and its application are obtained.

[0016] This invention utilizes the classic formula of Shenling Baizhu Powder, and extracts and separates the effective components using modern scientific technology to obtain an effective extract. Through appropriate formulation, it exerts its therapeutic effect. Pharmacological studies show that the traditional Chinese medicine composition of this invention can improve children's growth and development by enhancing the absorption of the spleen and stomach.

[0017] This invention provides a composition for promoting children's growth and development, comprising the following parts by weight of traditional Chinese medicine extracts: Chinese herbal extract A: 4-10 parts; Chinese herbal extract B: 3-7 parts; Chinese herbal extract C: 1-3 parts; Chinese herbal extract D: 2-6 parts. The preparation method of traditional Chinese medicine extract A includes the following steps: Codonopsis pilosula, Dioscorea opposita, Coix lacryma-jobi and Dolichos lablab were taken, pectinase and water were added, and microwave extraction was performed. The extract was collected and freeze-dried to obtain Chinese herbal extract A. The preparation method of traditional Chinese medicine extract B includes the following steps: Take Dangshen, add citric acid solution to keep the temperature at 30~35℃, soak, control the flow of infiltration, collect the infiltration liquid, freeze-drying to obtain traditional Chinese medicine extract B; The preparation method of traditional Chinese medicine extract C includes the following steps: Take Fuling and Jiegeng, extract with water for multiple times, combine the extract, filter, concentrate, freeze-drying to obtain traditional Chinese medicine extract C. The preparation method of traditional Chinese medicine extract D includes the following steps: Take Bai TianDou, Dangshen and Shanyao, put into a freeze ultra-micro powder grinder, control the temperature at 15~25℃, crush, collect the crushed powder to obtain traditional Chinese medicine extract D.

[0018] In the present application, the mass fraction of traditional Chinese medicine extract A can be any value between 4~10 parts, such as 4 parts, 5 parts, 6 parts, 7 parts, 9 parts, 10 parts, etc., preferably 4 parts, 7 parts, 10 parts, and most preferably 10 parts.

[0019] In the present application, the mass fraction of traditional Chinese medicine extract B can be any value between 3~7 parts, such as 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, etc., preferably 3 parts, 5 parts, 7 parts, and most preferably 3 parts.

[0020] In the present application, the mass fraction of traditional Chinese medicine extract C can be any value between 1~3 parts, such as 1 part, 2 parts, 3 parts, etc., and most preferably 3 parts.

[0021] In the present application, the mass fraction of traditional Chinese medicine extract D can be any value between 2~6 parts, such as 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, etc., preferably 2 parts, 4 parts, 6 parts, and most preferably 4 parts.

[0022] In the present application, the composition is most preferably composed of the following mass fractions of traditional Chinese medicine extracts: traditional Chinese medicine extract A 10 parts, traditional Chinese medicine extract B 3 parts, traditional Chinese medicine extract C 3 parts, and traditional Chinese medicine extract D 4 parts.

[0023] In the present application, the preparation method of traditional Chinese medicine extract A has no special requirements for process and raw material dosage, and can be extracted according to the conventional method in the art. The raw material dosage and process parameters given in the present application are the preferred dosage and preferred process parameters. For example, the temperature and time of freeze-drying, which are not given in the present application, can be set according to the conventional method in the art. In the preparation method of traditional Chinese medicine extract A in the present application, preferably, the mass ratio of radix codonopsis, Chinese yam, coix seed and white kidney bean is 8:7:4:4, the medicinal materials are weighed according to the proportion, crushed into fine powder, passed through 80 mesh, 3% pectinase of the total mass of medicinal materials is added, the pectinase is preferably SPE-010 type pectinase, mixed uniformly, 10 times the amount of water of the medicinal materials is added, placed in a microwave extractor, the microwave power is controlled at 320w, microwave extraction is performed for 60min, the extraction liquid is collected, freeze-dried, and the traditional Chinese medicine extract A is obtained.

[0024] In the present application, the preparation method of traditional Chinese medicine extract B has no special requirements for process and raw material dosage, and can be extracted according to the conventional method in the art. The raw material dosage and process parameters given in the present application are the preferred dosage and preferred process parameters. For example, the temperature and time of freeze-drying, which are not given in the present application, can be set according to the conventional method in the art. In the preparation method of traditional Chinese medicine extract B in the present application, preferably, the mass fraction of radix codonopsis is 10 parts, the medicinal materials are weighed according to the mass fraction, crushed into fine powder, passed through 80 mesh, 20 times the amount of 0.3% citric acid solution of the medicinal materials is added to maintain the temperature at 30~35℃, and the temperature is further preferably 30℃, soaked for 4h, the flow rate is controlled to 12h, the percolate is collected, freeze-dried, and the traditional Chinese medicine extract B is obtained.

[0025] In the present application, the preparation method of traditional Chinese medicine extract C has no special requirements for process and raw material dosage, and can be extracted according to the conventional method in the art. The raw material dosage and process parameters given in the present application are the preferred dosage and preferred process parameters. For example, the temperature and time of freeze-drying, which are not given in the present application, can be set according to the conventional method in the art. In the preparation method of traditional Chinese medicine extract C in the present application, preferably, the mass ratio of poria cocos and platycodon grandiflorum is 12:4, the medicinal materials are weighed according to the proportion, crushed into fine powder, passed through a 60 mesh sieve, 10 times the amount of water of the medicinal materials is added, extracted twice for 1.5h each time, the decoction is combined, filtered through a 400 mesh sieve, concentrated to 70℃, the relative density is 1.15~1.20 thick paste, freeze-dried, and the traditional Chinese medicine extract C is obtained.

[0026] In the present application, the preparation method of traditional Chinese medicine extract D has no special requirements for process and raw material dosage, and can be extracted according to the conventional method in the art. The raw material dosage and process parameters given in the present application are the preferred dosage and process parameters. For example, the process parameters such as the grinding time not given in the present application can be set according to the conventional method in the art. In the preparation method of traditional Chinese medicine extract D in the present application, preferably, the mass ratio of white kidney bean, radix codonopsis and yam is 8:4:4, which is ground into fine powder, then put into a refrigerated ultrafine powder grinder, the temperature is controlled at 15-25℃, preferably 20℃, and the powder is ground to 500 mesh, the ground powder is collected, and the traditional Chinese medicine extract D is obtained.

[0027] Finally, the traditional Chinese medicine extract A, the traditional Chinese medicine extract B, the traditional Chinese medicine extract C and the traditional Chinese medicine extract D are mixed in any ratio within the above mass ratio range, and the composition for promoting the growth and development of children is obtained.

[0028] The present application also provides a use of the composition of the present application in the preparation of a medicine for promoting the growth and development of children.

[0029] The technical solutions of the present application will be described in more detail below in conjunction with examples, and the protection scope of the present application is not limited by the following examples.

[0030] The traditional Chinese medicine extracts used in the following examples are prepared as follows: The preparation method of traditional Chinese medicine extract A comprises the following steps: Take radix codonopsis 8 parts, yam 7 parts, coix seed 4 parts and white kidney bean 4 parts, grind them into fine powder, pass through 80 mesh, add 3% pectinase of SPE-010 type to the amount of medicinal materials, mix uniformly, pour into a beaker, add 10 times the amount of water of medicinal materials, place in a microwave extractor, control the microwave power at 320w, microwave extract for 60min, collect the extract, freeze-dry to obtain a traditional Chinese medicine extract A for promoting the growth and development of children.

[0031] The preparation method of traditional Chinese medicine extract B comprises the following steps: Take radix codonopsis 10 parts, grind them into fine powder, pass through 80 mesh, add 0.3% citric acid solution to the amount of medicinal materials, keep the temperature at 30℃, soak for 4h, control the flow rate to 12h, collect the leaching liquid, freeze-dry to obtain a traditional Chinese medicine extract B for promoting the growth and development of children.

[0032] The preparation method of traditional Chinese medicine extract C comprises the following steps: Take 12 parts of poria cocos and 4 parts of platycodon root, grind them into fine powder, pass through a 60 mesh sieve, add 10 times the amount of water of medicinal materials to extract twice, each time for 1.5 hours, combine the decoction, filter through a 400 mesh sieve, concentrate to 70℃, the relative density of the thick paste is 1.15, freeze-dry to obtain a traditional Chinese medicine extract C for promoting the growth and development of children.

[0033] The preparation method of the traditional Chinese medicine extract D comprises the following steps: Take white kidney bean 8 parts, Dangshen 4 parts, and Chinese yam 4 parts, crush them into fine powder, then put them into a refrigerated ultrafine powder grinder, control the temperature at 20 DEG C, crush them to 500 meshes, collect the crushed powder, and obtain a traditional Chinese medicine extract D for promoting the growth and development of children.

[0034] The temperature and time of the freeze-drying in the preparation processes of the above extracts A-C can refer to the conventional settings in the prior art, and the present application has no special requirements thereon.

[0035] The screening experiment of each extract contained in the composition for promoting the growth and development of children of the present application is as follows: The composition of the present application is composed of the traditional Chinese medicine extract A, the traditional Chinese medicine extract B, the traditional Chinese medicine extract C, and the traditional Chinese medicine extract D, the mass fraction of the traditional Chinese medicine extract A, B, C and D is taken as a factor, three levels of each factor are determined, and an orthogonal test table is designed. The traditional Chinese medicine extracts are mixed according to the above-mentioned proportion, and nine groups of extracts I, II, III, IV, V, VI, VII, VIII and IX are prepared, and the mixture is obtained after being fully mixed. The nine groups of mixtures are subjected to efficacy evaluation research, the effect of promoting the growth and development of children is comprehensively evaluated, and the best group of extract is screened out.

[0036]

[0037] The preparation of the composition of the present application is as follows: The extracts are mixed according to the following table, and nine groups of compositions I, II, III, IV, V, VI, VII, VIII and IX are prepared.

[0038]

[0039] The following is a preparation example of the composition for promoting the growth and development of children of the present application.

[0040] Example 1: According to the mass fraction proportion, the prepared traditional Chinese medicine extract A 4 parts, the traditional Chinese medicine extract B 3 parts, the traditional Chinese medicine extract C 1 part, and the traditional Chinese medicine extract D 2 parts are fully mixed to obtain the composition I for promoting the growth and development of children of the present application.

[0041] Example 2: According to the mass fraction proportion, the prepared traditional Chinese medicine extract A 4 parts, the traditional Chinese medicine extract B 5 parts, the traditional Chinese medicine extract C 2 parts, and the traditional Chinese medicine extract D 4 parts are fully mixed to obtain the composition II for promoting the growth and development of children of the present application.

[0042] Example 3: By mass fraction: take the prepared traditional Chinese medicine extract A 4 parts, traditional Chinese medicine extract B 7 parts, traditional Chinese medicine extract C 3 parts, traditional Chinese medicine extract D 6 parts, and mix thoroughly to obtain the composition III for promoting growth and development of children of the present application.

[0043] Example 4: By mass fraction: take the prepared traditional Chinese medicine extract A 7 parts, traditional Chinese medicine extract B 3 parts, traditional Chinese medicine extract C 2 parts, traditional Chinese medicine extract D 6 parts, and mix thoroughly to obtain the composition IV for promoting growth and development of children of the present application.

[0044] Example 5: By mass fraction: take the prepared traditional Chinese medicine extract A 7 parts, traditional Chinese medicine extract B 5 parts, traditional Chinese medicine extract C 3 parts, traditional Chinese medicine extract D 2 parts, and mix thoroughly to obtain the composition V for promoting growth and development of children of the present application.

[0045] Example 6: By mass fraction: take the prepared traditional Chinese medicine extract A 7 parts, traditional Chinese medicine extract B 7 parts, traditional Chinese medicine extract C 1 part, traditional Chinese medicine extract D 4 parts, and mix thoroughly to obtain the composition VI for promoting growth and development of children of the present application.

[0046] Example 7: By mass fraction: take the prepared traditional Chinese medicine extract A 10 parts, traditional Chinese medicine extract B 3 parts, traditional Chinese medicine extract C 3 parts, traditional Chinese medicine extract D 4 parts, and mix thoroughly to obtain the composition VII for promoting growth and development of children of the present application.

[0047] Example 8: By mass fraction: take the prepared traditional Chinese medicine extract A 10 parts, traditional Chinese medicine extract B 5 parts, traditional Chinese medicine extract C 1 part, traditional Chinese medicine extract D 6 parts, and mix thoroughly to obtain the composition VIII for promoting growth and development of children of the present application.

[0048] Example 9: By mass fraction: take the prepared traditional Chinese medicine extract A 10 parts, traditional Chinese medicine extract B 7 parts, traditional Chinese medicine extract C 2 parts, traditional Chinese medicine extract D 2 parts, and mix thoroughly to obtain the composition IX for promoting growth and development of children of the present application.

[0049] Comparative Example 1 By mass fraction: take the prepared traditional Chinese medicine extract A 2 parts, traditional Chinese medicine extract B 9 parts, traditional Chinese medicine extract C 5 parts, traditional Chinese medicine extract D 4 parts, and mix thoroughly to obtain the composition for promoting growth and development of children.

[0050] Comparative Example 2 By mass fraction: take the above prepared traditional Chinese medicine extract A 6 parts, traditional Chinese medicine extract B 4 parts, traditional Chinese medicine extract C 8 parts, traditional Chinese medicine extract D 8 parts, and mix thoroughly to obtain a composition for promoting the growth and development of children.

[0051] Comparative example 3 By mass fraction: take the above prepared traditional Chinese medicine extract A 6 parts, traditional Chinese medicine extract B 4 parts, traditional Chinese medicine extract C 5 parts, traditional Chinese medicine extract D 8 parts, and mix thoroughly to obtain a composition for promoting the growth and development of children.

[0052] The following are twelve groups of compositions prepared in Examples 1-9 and Comparative Examples 1-3 of the present application, and comparative experimental studies on their pharmacodynamics.

[0053] I. Animal grouping, modeling and administration After 150 3-week-old young mice were adaptively fed for one week, they were randomly divided into 15 groups, 10 mice in each group, which were blank group, Chinese patent medicine group (Shenling Baizhu Powder group, 4 g / kg), western medicine group (calcium carbonate group, 0.5 g / kg), Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Example 7 group, Example 8 group, Example 9 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group. Except for the blank group, each administration group was respectively given 4 g / kg of the corresponding medicine by gavage, i.e., Examples 1-9 groups were respectively given 0.11 g / kg (equivalent to 0.011 g / mL of the medicine prepared by using physiological saline) of the compositions I-IX prepared in the above examples of the present application, Comparative Examples 1-3 groups were respectively given 0.11 g / kg of the compositions prepared in Comparative Examples 1-3, the Chinese patent medicine group and the western medicine group were given the above administration amount by gavage, and the blank group was given the same volume of physiological saline by gavage. After 28 days of continuous administration, the mice were fasted for 12 h, dissected, and the complete digestive tract from the stomach to the end of the rectum was taken out. The contents of the intestinal tract were washed with physiological saline, dried with filter paper, weighed, and stored at -80℃. The right femur was taken out, washed with physiological saline, dried with filter paper, measured for length, and the thymus and liver tissues were taken out, washed with physiological saline to remove blood stains, dried with filter paper, and weighed.

[0054] II. Determination of growth indicators Body weight gain (g) = final body weight (g) - initial body weight (g) III. Measurement of femur length The right femur was taken out, washed with physiological saline, dried with filter paper, measured with a vernier caliper, and the length was recorded.

[0055] IV. Determination of α-amylase activity The above stored jejunum was taken out, about 0.1 g of tissue was taken out at the same position, homogenized, and detected under an enzyme marker. The activity of α-amylase was determined according to the method of the kit.

[0056] V. Immune index detection Thymus organ index (%) = thymus weight (g) / body weight (g) 100%; Liver organ index (%) = liver weight (g) / body weight (g) 100%; VI. Statistical analysis Excel statistical analysis method was used for the need of data analysis. Data was expressed as The sample means of multiple groups were compared by one-way analysis of variance. P<0.05, P<0.01, P<0.001 were considered statistically significant.

[0057] Experimental results 1. Effect on the body weight of young mice Compared with the blank group, the body weight of mice in the Chinese medicine group and the western medicine group increased significantly (P<0.05), and the body weight of mice in the example 1-9 groups increased significantly (P<0.05). From the results of the increase in the body weight of mice, it can be seen that in the composition of the example 1-9 groups, the example 7 group is better than the Chinese medicine group and the comparative example group, see Table 1 for details.

[0058] Table 1 Effect on the body weight of young mice (n=10)

[0059] Note: The shoulder mark represents that after variance analysis-two comparison, the same column letter is different, which represents significant difference (P<0.05), and the same column letter is the same, which represents no significant difference (P>0.05).

[0060] 2. Effect on the femur length of young mice Compared with the blank group, the femur length of mice in the Chinese medicine group and the western medicine group increased significantly (P<0.05), and the femur length of mice in the example 1-9 groups increased significantly (P<0.05). From the results of the femur length of mice, it can be seen that in the composition of the example 1-9 groups, the example 7 group is better than the Chinese medicine group and the comparative example group, see Table 2 for details.

[0061] Table 2 Effect on the femur length of young mice (n=10)

[0062] Note: The shoulder mark represents that after variance analysis-two comparison, the same column letter is different, which represents significant difference (P<0.05), and the same column letter is the same, which represents no significant difference (P>0.05).

[0063] 3. Effect on the activity of α-amylase Compared with the blank group, the alpha-amylase activity of the mice in the Chinese medicine group and the western medicine group was significantly increased (P<0.05), and the alpha-amylase activity of the mice in the example 1-9 groups was significantly increased (P<0.05). It can be seen from the alpha-amylase activity of the mice that in the compositions of the example 1-9 groups, the example 7 group is better than the Chinese medicine group and the comparative example group, and the specific see table 3.

[0064] Table 3 Effect on alpha-amylase activity of young mice n=10

[0065] Note: The superscript represents that after variance analysis-two comparison, the same column letter is different, which represents significant difference (P<0.05), and the same column letter is the same, which represents no significant difference (P>0.05).

[0066] 4. Effect on immune organ index Compared with the blank group, the thymus index and liver index of the mice in the Chinese medicine group and the western medicine group were significantly increased (P<0.05), and the thymus index and liver index of the mice in the example 1-9 groups were significantly increased (P<0.05). It can be seen from the thymus index and liver index of the mice that in the compositions of the example 1-9 groups, the example 7 group is better than the Chinese medicine group and the comparative example group, and close to the western medicine group, and the specific see table 4.

[0067] Table 4 Effect on immune organ index of young mice n=10

[0068] Note: The superscript represents that after variance analysis-two comparison, the same column letter is different, which represents significant difference (P<0.05), and the same column letter is the same, which represents no significant difference (P>0.05).

[0069] In combination with the above tables 1-4, the compositions of the example 1-9 groups provided by the application are used as growth indexes by the weight gain, femur length, alpha-amylase activity and immune organ index. It can be seen from the results that the mouse weight gain can be increased, the skeletal muscle growth and development can be promoted, the alpha-amylase activity can be promoted, the starch hydrolysis is beneficial, the absorption of nutrients is promoted, the development and maturation of immune organs and liver are promoted, the immune function of the body is promoted, the immune function of the body is enhanced, and the example 7 group is the best, and under the same dose condition, it is better than the Chinese medicine group and other composition groups.

[0070] The foregoing description of the disclosed embodiments enables one of ordinary skill in the art to make or use the application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein can be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A composition for promoting growth and development in children, characterized in that, The composition comprises the following components by mass: 4-10 parts of Chinese medicine extract A, 3-7 parts of Chinese medicine extract B, 1-3 parts of Chinese medicine extract C, and 2-6 parts of Chinese medicine extract D. The preparation method of the Chinese medicine extract A comprises the following steps: Take Dangshen, Shanyao, Yiyiren and Baibiandou, add pectinase and water, and perform microwave extraction, collect the extraction liquid, and freeze-dry to obtain the Chinese medicine extract A; The preparation method of the Chinese medicine extract B comprises the following steps: Take Dangshen, add citric acid solution to maintain the temperature at 30-35℃, soak, control the flow rate of percolation, collect the percolate, and freeze-dry to obtain the Chinese medicine extract B; The preparation method of the Chinese medicine extract C comprises the following steps: Take Fuling and Jiegeng, perform multiple water extraction, combine the extraction liquids, filter, concentrate, and freeze-dry to obtain the Chinese medicine extract C; The preparation method of the Chinese medicine extract D comprises the following steps: Take Baibiandou, Dangshen and Shanyao, put them into a frozen ultrafine powder grinder, control the temperature at 15-25℃, grind, and collect the ground powder to obtain the Chinese medicine extract D.

2. The composition for promoting growth and development of children according to claim 1, characterized by, The composition comprises the following components by mass: 10 parts of Chinese medicine extract A, 3 parts of Chinese medicine extract B, 3 parts of Chinese medicine extract C, and 4 parts of Chinese medicine extract D.

3. The composition for promoting growth and development of children according to claim 1, characterized by, In the preparation method of the Chinese medicine extract A, the mass ratio of Dangshen, Shanyao, Yiyiren and Baibiandou is 8:7:4:

4.

4. The composition for promoting growth and development of children according to claim 1, characterized by, In the preparation method of the Chinese medicine extract A, the power of microwave extraction is 320w, and the microwave extraction time is 60min.

5. The composition for promoting growth and development of children according to claim 1, characterized in that, In the preparation method of the Chinese medicine extract B, the mass of Dangshen is 10 parts.

6. The composition for promoting growth and development of children according to claim 1, characterized by, In the preparation method of the Chinese medicine extract B, the temperature is maintained at 30℃, the soaking time is 4h, and the flow rate of percolation is controlled for 12h.

7. The composition for promoting growth and development of children according to claim 1, characterized by, In the preparation method of the Chinese medicine extract C, the mass ratio of Fuling and Jiegeng is 12:4, and the water extraction is performed for 2 times, each time for 1.5h.

8. The composition for promoting growth and development of children according to claim 1, characterized by, In the preparation method of the Chinese medicine extract D, the mass ratio of Baibiandou, Dangshen and Shanyao is 8:4:4, and the temperature is controlled at 20℃, and the grinding is performed to 500 mesh.

9. Use of the composition of any one of claims 1-8 in the preparation of a medicine for promoting the growth and development of children.

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