A method for producing yellow humic acid slow-release organic fertilizer by using tylosin dregs

Abstract

The application discloses a method for producing yellow fermentation acid slow-release organic fertilizer by using tylosin bacterial residue, and the method comprises the following steps: crushing the tylosin bacterial residue into powder by using a cell wall breaking machine; adding gamma-manganese dioxide and lignite in sequence, stirring and mixing uniformly, and fully contacting and reacting; irradiating and treating the obtained mixed system by using a high-energy electron beam to obtain yellow fermentation acid organic fertilizer; and compounding the obtained product with diatomite, and granulating after cooling to obtain the yellow fermentation acid slow-release organic fertilizer. The harmless treatment and resource utilization of the tylosin bacterial residue are realized, the product meets the actual production and application requirements, the product has good fertilizer efficiency, the residual antibiotics are removed synchronously, and the product has the dual functions of fertilizer efficiency and antibiotic removal.

Description

Technical Field

[0001] This invention relates to a method for producing slow-release humic acid organic fertilizer using tylosin bacterial residue, belonging to the field of solid waste disposal and resource utilization technology. Background Technology

[0002] The widespread use of antibiotics has played a vital role in promoting animal growth and preventing diseases, but it has also led to increased emissions of antibiotic production waste and heightened environmental risks. The production of antibiotics typically generates large amounts of fermentation residues containing organic matter and residual antibiotics. Improper handling of these residues can easily cause soil pollution, the spread of resistance genes, and ecosystem damage.

[0003] Tylosin is a broad-spectrum macrolide antibiotic produced by Streptomyces and widely used in livestock and poultry farming. The tylosin fermentation residue produced during its production mainly consists of bacterial residues, organic culture medium components, fermentation byproducts, and residual antibiotics. It has a high organic matter content, thus possessing the potential for utilization as organic fertilizer from a resource perspective. However, if the residual antibiotics and fermentation byproducts in the residue are directly discharged or applied to farmland without proper treatment, it can lead to stunted crop growth, imbalanced soil microbial communities, and even the spread of antibiotic resistance, causing environmental problems.

[0004] Current incineration-based treatment methods suffer from drawbacks such as high energy consumption and the generation of toxic gases. Some existing studies have attempted to utilize tylosin fermentation residue to produce organic fertilizer through aerobic composting and anaerobic fermentation, but these methods suffer from drawbacks such as incomplete antibiotic residue degradation, uneven nutrient release, and low fertilizer efficiency. Fulvic acid can improve fertilizer efficiency, promote crop growth, and improve soil physicochemical properties. High-energy electron beam irradiation of manganese dioxide can continuously generate various highly reactive species (intermediate active manganese, hydroxyl radicals, and hydrogen radicals), enabling deep removal of antibiotics. Lignite contains a large amount of fulvic acid and has adsorption capacity, while diatomaceous earth has slow-release properties; combining the two achieves a dual function of "fertilizer-slow-release." Therefore, a new method for the resource-based disposal of tylosin fermentation residue is urgently needed to achieve its green reuse in the agricultural field and promote the reduction, harmlessness, and high-value transformation of antibiotic fermentation waste. Summary of the Invention

[0005] The technical problem to be solved by this invention is that the existing technology has problems such as low removal rate of residual antibiotics in tylosin bacterial residue, long resource utilization cycle, and low fertilizer efficiency.

[0006] To address the above problems, this invention provides a method for producing slow-release humic acid organic fertilizer using tylosin inoculum residue, comprising the following steps:

[0007] Step 1): Grind the tylosin residue into powder using a high-speed blender;

[0008] Step 2): Add γ-manganese dioxide and lignite to the tylosin residue obtained in Step 1) in sequence, stir and mix evenly and allow for full contact and reaction;

[0009] Step 3): The mixed system obtained in Step 2) is irradiated with a high-energy electron beam to obtain humic acid type organic fertilizer;

[0010] Step 4): Combine the product obtained in Step 3) with diatomaceous earth, cool and granulate to obtain humic acid slow-release organic fertilizer.

[0011] Preferably, in step 1), the tylosin residue has a moisture content of 70-75 wt% and a fineness of 50-75 mesh.

[0012] Preferably, in step 2), the lignite has a moisture content of 10-12 wt%, a purity of 80-85%, and a fineness of 115-125 mesh.

[0013] Preferably, in step 3), the irradiation energy of the high-energy electron beam is 2-6 MeV, the beam current intensity is 40-70 mA, and the dose is 30-50 kGy.

[0014] Preferably, in step 4), the fineness of the diatomaceous earth is 150-200 mesh.

[0015] Preferably, in step 4), the temperature is cooled to 40-60°C.

[0016] Preferably, the mass ratio of tylosin bacterial residue, γ-manganese dioxide, and lignite is 200-300:8-12:20-30; the mass ratio of the product in step 4) to diatomaceous earth is 4:1.

[0017] This invention also provides a slow-release organic fertilizer of humic acid prepared by the above method.

[0018] Preferably, the fulvic acid slow-release organic fertilizer has a particle size of 2-3 mm and contains 8-20 wt% fulvic acid derived from tylosin bacterial residue.

[0019] This invention also provides the application of the above-mentioned slow-release organic fertilizer of fulvic acid in planting, with an application rate of 40-100 kg / mu.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The composting cycle of the mushroom residue is 1 hour, which is more than 720 times faster than aerobic composting (30-60 days);

[0022] 2. The removal rate of residual tylosin in the treated mushroom residue reaches 98-100%, and the sterilization rate reaches 98-99.98%.

[0023] 3. The product contains fulvic acid with a content as high as 8%-20%. The slow-release organic fertilizer increases the yield of Shanghai bok choy by 26.71%-46.84%, plant height by 18.22%-33.42%, and soil organic matter content by 8.65%-14.18%. Attached Figure Description

[0024] Figure 1 A schematic diagram of the method provided in Example 2;

[0025] Figure 2 A comparison of the growth of potted Shanghai bok choy prepared in Example 2 with traditional organic fertilizer and a blank control group;

[0026] Figure 3 Comparison of yield, plant height, root length, and chlorophyll content of Shanghai bok choy prepared in Example 2 with traditional organic fertilizer and blank group;

[0027] Figure 4 Comparison of live bacteria plate tests between the humic acid slow-release organic fertilizer prepared in Example 2, tylosin bacterial residue, and the blank group;

[0028] Figure 5 This is a comparison chart of residual antibiotic content in tylosin bacterial residues at different reaction times in Examples 1-3;

[0029] Figure 6 The image shows a three-dimensional fluorescence comparison between the humic acid slow-release organic fertilizer prepared in Examples 1-3 and the tylosin residue before treatment. Detailed Implementation

[0030] To make the present invention more apparent and understandable, preferred embodiments are described in detail below with reference to the accompanying drawings.

[0031] Example 1

[0032] A method for producing humic acid slow-release organic fertilizer using tylosin inoculum residue includes the following steps:

[0033] (1) 200g of tylosin bacterial residue (moisture content 70 wt%) was crushed to 50 mesh using a wall-breaking machine to obtain powdered bacterial residue;

[0034] (2) Add 8g of γ-manganese dioxide (100nm) and 20g of lignite (10wt% moisture content, 80% purity, 115 mesh) to the powdered tylosin residue obtained in step (1) in sequence, and stir to mix the materials evenly and allow them to fully react.

[0035] (3) The mixture in step (2) was irradiated with a high-energy electron beam (2MeV, 40mA, 30kGy) to obtain humic acid organic fertilizer;

[0036] (4) The product obtained in step (3) is mixed with diatomaceous earth (150 mesh) at a mass ratio of 4:1 and left to stand. After cooling to 40°C, it is granulated to obtain tylosin bacterial residue source humic acid slow-release organic fertilizer with a diameter of 2-3 mm.

[0037] The humic acid slow-release organic fertilizer prepared in (4) was evenly applied to the soil surface at a rate of 40 kg / mu, and Shanghai bok choy was planted after rotary tillage. Compared with the same amount of traditional organic fertilizer, the yield of Shanghai bok choy increased by 26.71%, the plant height increased by 18.22%, and the soil organic matter content increased by 8.65%.

[0038] Example 2

[0039] A method for producing humic acid slow-release organic fertilizer using tylosin inoculum residue includes the following steps:

[0040] (1) 250g of tylosin residue (moisture content 73 wt%) was crushed to 65 mesh using a wall-breaking machine to obtain powdered residue;

[0041] (2) Add 10g of γ-manganese dioxide (130nm) and 25g of lignite (moisture content 11 wt%, purity 83%, 120 mesh) to the powdered tylosin residue obtained in step (1) in sequence, and stir to mix the materials evenly and allow them to fully contact and react.

[0042] (3) The mixture in step (2) was irradiated with a high-energy electron beam (4MeV, 55mA, 40kGy) to obtain humic acid organic fertilizer;

[0043] (4) The product obtained in step (3) is mixed with diatomaceous earth (180 mesh) at a mass ratio of 4:1 and left to stand. After cooling to 50°C, it is granulated to obtain tylosin bacterial residue source humic acid slow-release organic fertilizer with a diameter of 2-3 mm.

[0044] The humic acid slow-release organic fertilizer prepared in (4) was evenly applied to the soil surface at a rate of 70 kg / mu, and Shanghai bok choy was planted after rotary tillage. Compared with the same amount of traditional organic fertilizer, the plant height of Shanghai bok choy increased by 33.42% (see Figure 2 Production increased by 46.84% (see) Figure 3 The soil organic matter content increased by 14.18%, and it also had a good antibacterial effect (see...). Figure 4 ).

[0045] The results of the soil index comparison test between the prepared humic acid slow-release organic fertilizer and the blank and traditional organic fertilizer are shown in Table 1.

[0046] Table 1

[0047]

[0048] Example 3

[0049] A method for producing humic acid slow-release organic fertilizer using tylosin inoculum residue includes the following steps:

[0050] (1) 300g of tylosin bacterial residue (moisture content 75 wt%) was crushed to 75 mesh using a wall-breaking machine to obtain powdered bacterial residue;

[0051] (2) Add 12g of γ-manganese dioxide (150nm) and 30g of lignite (moisture content 12 wt%, purity 85%, 125 mesh) to the powdered tylosin residue obtained in step (1) in sequence, and stir to make the materials mix evenly and fully contact each other for reaction;

[0052] (3) The mixture in step (2) was irradiated with a high-energy electron beam (6MeV, 70mA, 50kGy) to obtain humic acid organic fertilizer;

[0053] (4) The product obtained in step (3) is mixed with diatomaceous earth (200 mesh) at a mass ratio of 4:1 and left to stand. After cooling to 60°C, it is granulated to obtain tylosin bacterial residue source humic acid slow-release organic fertilizer with a diameter of 2-3 mm.

[0054] The humic acid slow-release organic fertilizer prepared in (4) was evenly applied to the soil surface at a rate of 100 kg / mu, and Shanghai bok choy was planted after rotary tillage. Compared with the same amount of traditional organic fertilizer, the yield of Shanghai bok choy increased by 42.12%, the plant height increased by 28.36%, and the soil organic matter content increased by 12.58%.

[0055] The residual antibiotic content in the tylosin inoculum residue of the fulvic acid slow-release organic fertilizer prepared in Examples 1-3 at different reaction times was detected (see...). Figure 5 Furthermore, a three-dimensional fluorescence comparison was performed between the humic acid slow-release organic fertilizer prepared in Examples 1-3 and the tylosin residue before treatment (see [reference]). Figure 6 ).Depend on Figure 5 , 6 It can be seen that, among them, Example 2 has the best effect in promoting soil improvement.

Claims

1. A method for producing slow-release humic acid organic fertilizer using tylosin inoculum residue, characterized in that, Includes the following steps: Step 1): Grind the tylosin residue into powder using a high-speed blender; Step 2): Add γ-manganese dioxide and lignite sequentially to the tylosin residue obtained in Step 1), stir and mix evenly to ensure full contact and reaction; the mass ratio of the tylosin residue, γ-manganese dioxide, and lignite is 200-300:8-12:20-30. Step 3): The mixed system obtained in Step 2) is irradiated with a high-energy electron beam to obtain humic acid type organic fertilizer; Step 4): Mix the product obtained in Step 3) with diatomaceous earth at a mass ratio of 4:1, cool and granulate to obtain humic acid slow-release organic fertilizer.

2. The method as described in claim 1, characterized in that, In step 1), the tylosin residue has a moisture content of 70-75 wt% and a fineness of 50-75 mesh.

3. The method as described in claim 1, characterized in that, In step 2), the lignite has a moisture content of 10-12 wt%, a purity of 80-85%, and a fineness of 115-125 mesh.

4. The method as described in claim 1, characterized in that, In step 3), the irradiation energy of the high-energy electron beam is 2-6 MeV, the beam current intensity is 40-70 mA, and the dose is 30-50 kGy.

5. The method as described in claim 1, characterized in that, In step 4), the fineness of the diatomaceous earth is 150-200 mesh.

6. The method as described in claim 1, characterized in that, In step 4), the temperature is cooled to 40-60°C.

7. A slow-release organic fertilizer of humic acid prepared by the method according to any one of claims 1-6.

8. The fulvic acid slow-release organic fertilizer as described in claim 7, characterized in that, The slow-release organic fertilizer containing fulvic acid has a particle size of 2-3 mm and contains 8-20 wt% fulvic acid derived from tylosin bacterial residue.

9. The application of the humic acid slow-release organic fertilizer according to claim 7 or 8 in planting, characterized in that, The application rate is 40-100 kg / mu.

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