A high-purity culture system for *Tetranychus spp.* strain and its culture method

Abstract

This invention belongs to the field of biological culture equipment technology, and specifically relates to a high-purity culture system and method for *Pterygodium japonicum* strains. The culture system includes a culture device and a control module. The culture device includes a rectangular culture chamber and an integrally formed equilateral triangular prism-shaped ceiling. This invention achieves uniform three-dimensional illumination by symmetrically arranging linear blue light strips on three inner surfaces. A light adjustment module progressively increases the blue light irradiance intensity over time to match the light requirements of *Pterygodium japonicum* during its growth cycle. An integrated industrial camera monitors mycelial coverage in real time, and temperature, humidity, micro-electric field, and negative pressure ventilation are used for coordinated control. Furthermore, the control module dynamically adjusts light, airflow, and nutrient supply based on sensor data, forming a closed-loop intelligent control system. This invention effectively improves the uniformity, purity, and anti-contamination ability of mycelial growth, and is suitable for high-efficiency, industrial-scale culture of *Pterygodium japonicum*.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of biological culture equipment, and particularly relates to a high-purity culture system for a white irpex lacteus strain and a culture method thereof. BACKGROUND

[0002] In the industrialized culture field of edible fungi and medicinal fungi, a closed constant-temperature incubator or a clean culture room combined with artificial inoculation is generally used for mycelium propagation, which usually includes a rectangular culture cavity provided with an LED lighting system, an independent temperature and humidity control module, and a gas exchange mechanism based on timed ventilation, so as to meet the basic needs of strain growth by setting fixed light period and environmental parameters. Some systems also integrate basic ultraviolet sterilization devices and sliding culture racks to improve operation convenience and reduce the risk of external pollution. In addition, in order to realize a certain degree of process monitoring, some high-end equipment begins to introduce cameras to record the growth state, and the overall technical development presents a trend of evolution towards automation and environmental control.

[0003] However, the existing culture system still has obvious limitations in realizing high-purity and high-consistency mycelium culture. Due to uneven light distribution, the mycelium growth rate differs greatly, making it difficult to form a dense and uniform colony structure. The fixed light intensity and the lack of dynamic feedback regulation mechanism make the system unable to adapt to the physiological property fluctuations of different batches of strains. At the same time, the unreasonable air flow organization design easily produces vortex or local positive pressure area, increasing the risk of external pollutant backflow and affecting the stability of the culture environment. In addition, the existing sterilization and environmental control are mostly operated independently, lacking the ability of multi-factor collaborative control, which makes it difficult to effectively inhibit the growth of miscellaneous bacteria and ensure the continuous and healthy growth of mycelium in actual operation. SUMMARY

[0004] (I) Technical problems to be solved

[0005] In view of the problems of uneven light distribution, single environmental regulation, lack of dynamic feedback mechanism and susceptibility to external pollution in the existing fungus culture technology, the present application aims to provide a high-purity culture system integrated with spatially symmetric light source layout, gradient light intensity regulation, multi-factor collaborative control and intelligent closed-loop feedback, and a culture method thereof, to realize efficient, stable and high-purity directional growth of white irpex lacteus mycelium.

[0006] (II) Technical solutions

[0007] The present application is achieved by the following technical solutions: the present application provides a high-purity culture system for a white irpex lacteus strain, which comprises a culture device and a control module, the culture device comprises a rectangular culture chamber and an integrally formed equilateral triangular prism-shaped ceiling provided at the top of the culture chamber;

[0008] A set of linear blue light strips are arranged on each of the three inner sides of the equilateral triangular prism-shaped ceiling, wherein the first set is arranged along the top edge, the second and third sets are symmetrically arranged along the upper edges of the two inclined sides, and the three sets of light strips are spatially symmetrically distributed;

[0009] The central wavelength of each set of linear blue light strips is 450±5nm;

[0010] The culture device further integrates a light adjustment module, which is connected with the control module and configured to provide a time-gradient increasing blue light irradiation to the Leucophenga albiceps strain inoculation area during the culture process;

[0011] The gradient-increasing blue light irradiation gradually increases from 4-6 μmol / m² / s in the initial stage to 8-11 μmol / m² / s in the stable stage, and for the Leucophenga albiceps strain, it is more specific: 5±0.5 μmol / m² / s on the first day, 7±0.5 μmol / m² / s on the second day, 9±0.5 μmol / m² / s on the third day, and 10±0.5 μmol / m² / s from the fourth to the seventh day.

[0012] Preferably, the inner side wall of the rectangular culture chamber is provided with symmetrically arranged sliding rails for slidably supporting the rectangular culture dish;

[0013] The rectangular culture dish is slidably connected with the sliding rails through a sliding plate;

[0014] The rectangular culture dish is also linked with the closing door through a connecting rod, so that the rectangular culture dish is pulled in or out of the rectangular culture chamber when the closing door is pulled or pushed.

[0015] Preferably, the side of the equilateral triangular prism-shaped ceiling is provided with an industrial camera, which is signal-connected with the control module and has a shooting field of view completely covering the top surface of the rectangular culture dish for real-time collection of mycelium growth images;

[0016] The light adjustment module is configured to dynamically compensate and adjust the preset blue light irradiation intensity according to the mycelium coverage data collected by the industrial camera, with a floating range of ±1.0 μmol / m² / s on the basis of the gradient value, to adapt to the growth differences of different batches of strains.

[0017] Preferably, the bottom of the rectangular culture dish is provided with four electrode sheets, which are uniformly distributed around the central area and inclined to the center with an inclination angle of 15°±2°;

[0018] The culture device further integrates an electrode adjustment module, which is connected with the control module and configured to apply a direct current voltage of 1.2±0.05V to each electrode sheet to form a weak electric field on the surface of the culture medium to promote the directional growth of mycelium.

[0019] Preferably, the bottom of the rectangular culture chamber is provided with two rows of equally spaced through air inlets, which are located on the lower sides of the rectangular culture dish.

[0020] The through-type air inlet is connected to the negative pressure fan through the air inlet pipe. The negative pressure fan introduces external air through the filter pipe (the filter pipe is equipped with a HEPA high-efficiency filter and an activated carbon layer to remove particulate matter and volatile organic compounds with a particle size ≥0.3μm in the air) and sends it into the air inlet pipe after filtration.

[0021] The negative pressure fan is connected to the control module to maintain the air pressure in the culture chamber at -40±2Pa. During the operation of the negative pressure fan, the airflow velocity is maintained between 0.15 and 0.25 m / s to achieve laminar airflow and avoid disturbing mycelial growth.

[0022] The rectangular culture chamber has multiple loop-shaped air outlet slots arranged in a loop pattern in the middle of its bottom;

[0023] The bottom center of the multi-loop air outlet groove is provided with a reverse air intake structure, which is connected to the air outlet groove to prevent unfiltered air from the outside from flowing back into the air outlet channel.

[0024] The reverse air intake structure includes an inverted conical flow guide cavity and a one-way valve located at its bottom;

[0025] The upper end of the inverted conical guide cavity is connected to the multi-loop air outlet groove, and the lower part forms a closed exhaust channel;

[0026] The one-way valve is a spring-loaded duckbill valve or flap valve, configured to allow gas to be discharged from the culture chamber to the outside in only one direction. It automatically closes when the external pressure is higher than the internal pressure to prevent unfiltered air from flowing back in.

[0027] Preferably, a plurality of sterilization lamps are provided in the rectangular culture chamber on both sides of the rectangular culture dish. The sterilization lamps emit ultraviolet light or short-wave visible light for periodic sterilization of the area entering and exiting.

[0028] The germicidal lamp is connected to the control module, and can be started, stopped, or have its irradiation parameters adjusted under control.

[0029] The germicidal lamps include UVC ultraviolet lamps (wavelength 254±5 nm) and violet LEDs (wavelength 405±10 nm), which are turned on and off alternately or synchronously.

[0030] Preferably, a temperature sensor and a humidity sensor are provided in the middle of the rectangular culture dish. Both sensors are connected to the control module for real-time monitoring of temperature and humidity changes in the culture environment.

[0031] Preferably, the rectangular petri dish is provided with a spray structure at the top;

[0032] The slide plate is equipped with a solution injection device, which is connected to the spraying structure through a pipeline and is used to quantitatively spray sterile water or nutrient solution into the petri dish.

[0033] The solution injection device is connected to the control module and is controlled by the module to perform the spraying operation.

[0034] The solution injection device is connected to two storage tanks, namely a sterile water storage tank and a nutrient solution storage tank, which are connected to the spraying structure through an electromagnetic switching valve;

[0035] The control module selectively triggers water replenishment (sterile water) or nutrient solution replenishment operations based on the combined data analysis results of the humidity sensor and temperature sensor.

[0036] Preferably, the control module is mounted on the culture device body and is connected to an external computer via a communication interface;

[0037] The control module is configured to receive monitoring data from an industrial camera, a temperature sensor, a humidity sensor, and a dissolved oxygen detection device, and to output corresponding control commands to the lighting adjustment module, the electrode adjustment module, the negative pressure fan, the germicidal lamp, and the solution injection device to achieve closed-loop intelligent control.

[0038] Preferably, the outer edge of the closed door is provided with a sealing ring (usually made of silicone) for sealing with the open side of the rectangular culture chamber, and so that the closed door can be engaged with the open side, thus fixing the rectangular culture dish.

[0039] Preferably, the rectangular culture chamber is provided with a base frame at the bottom.

[0040] On the other hand, the present invention also provides a method for high-purity cultivation of *Tetranychus spp.* strains, implemented based on the cultivation system described in any of the above claims, comprising the following steps:

[0041] S1: Inoculate the white rake tooth bacterium strain onto the surface of a solid culture medium in a rectangular petri dish;

[0042] S2: Start the control module and control the light adjustment module to output blue light irradiation according to the preset program: 5±0.5μmol / m² / s on day 1, 7±0.5μmol / m² / s on day 2, 9±0.5μmol / m² / s on day 3, and maintain 10±0.5μmol / m² / s from day 4 to day 7.

[0043] S3: Turn on the negative pressure fan to supply air to the culture chamber through the filter pipe and air intake pipe, and adjust the fan speed to maintain a negative pressure state of −40±2Pa in the chamber;

[0044] S4: The control electrode adjustment module applies a DC voltage of 1.2±0.05V to the four tilted electrode plates to establish a directional micro-electric field in the culture medium area;

[0045] S5: Continuously acquires images of mycelial growth using an industrial camera, analyzing mycelial coverage and breakage rate; simultaneously acquires environmental data through temperature and humidity sensors; and executes the following feedback control based on the monitoring results:

[0046] If the industrial camera detects that the mycelial breakage rate exceeds 5% twice in a row, the light adjustment module and negative pressure fan will be suspended for 1 hour before being resumed.

[0047] If the humidity is lower than the set threshold, the solution injection device is triggered to replenish sterile water or nutrient solution through the spray structure.

[0048] If the ratio of the ultraviolet intensity at the outlet to the inlet of the germicidal lamp is less than 0.55±0.02, it indicates a decrease in sterilization efficiency, and the lamp will automatically increase its irradiation intensity or extend its operating time.

[0049] Adjust the speed of the negative pressure fan in real time to ensure that the pressure in the culture chamber is constant at −40±2Pa;

[0050] S6: After 7 days of cultivation, the sliding door is pulled out to move the slide plate and rectangular culture dish out synchronously, and the culture product is taken out, completing the high-purity culture process.

[0051] Preferably, it also includes the following intelligent control steps:

[0052] At fixed times each day, industrial cameras capture images of mycelium, and a built-in algorithm calculates the daily mycelium coverage growth rate. If the growth rate is less than 80% of the expected threshold, the cultivation time for days 4–7 is extended by 1–2 days.

[0053] After each solution spraying, wait 10 minutes before turning on the negative pressure fan to prevent the droplets from being drawn away too quickly, which could lead to uneven wetting.

[0054] After the culture is completed, the control module generates an electronic culture report that includes temperature and humidity curves, light records, electric field application time, sterilization cycle, and image change sequence, and uploads it to an external computer or cloud platform via a communication interface.

[0055] (III) Beneficial Effects

[0056] This invention achieves three-dimensional uniform illumination of the cultivation area by constructing an integrated equilateral triangular prism-shaped canopy and setting spatially symmetrical blue light strips on its three inner sides, effectively overcoming the edge shadows and illuminance attenuation problems caused by traditional planar light sources. Combined with a specific wavelength (450±5nm) and a time-gradient increasing illumination strategy, it matches the photoresponse characteristics of *Tetranychus spp.*, significantly promoting mycelial densification and improving cell purity and biomass accumulation rate. The system introduces an industrial camera to monitor mycelial development in real time and dynamically adjusts light intensity parameters based on image data, forming a perception-feedback-control closed loop, which improves the adaptability to batch differences between different mycelial species. In addition, the collaborative design of the negative pressure-guided laminar flow air intake system, the bottom multi-loop air outlet, and the reverse air intake structure ensures the stability of the clean airflow organization, prevents backflow of external pollutants, and further enhances the reliability of the cultivation environment.

[0057] This invention achieves multi-dimensional coupled control of optical excitation, electric field guidance, airflow purification and intelligent feedback, which not only improves the consistency and success rate of white tooth fungus cultivation, but also provides reliable technical support for the industrial and standardized production of high-value-added edible fungi and medicinal fungi. Attached Figure Description

[0058] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0059] Figure 1 This is a system block diagram of the present invention.

[0060] Figure 2 This is a schematic diagram of the structure of the culture device of the present invention.

[0061] Figure 3 This is a schematic diagram of the internal structure of the rectangular culture chamber of the present invention.

[0062] Figure 4 This is a schematic diagram of the inner bottom structure of the rectangular culture chamber of the present invention.

[0063] Figure 5 This is a schematic diagram of the bottom structure of the rectangular culture chamber of the present invention.

[0064] The labels in the attached diagram are as follows: 1-Cultivation device, 11-Rectangular cultivation chamber, 12-Equilateral triangular prism-shaped ceiling, 13-Linear blue light strip, 14-Sealed door, 15-Connecting rod, 16-Slide rail, 17-Slide plate, 18-Solution injection device, 19-Rectangular culture dish, 110-Electrode plate, 111-Temperature sensor element, 112-Humidity sensor element, 113-Spraying structure, 114-Through-type air inlet, 115-Negative pressure fan, 116-Air inlet pipe, 117-Sterilization lamp, 118-Multi-loop air outlet, 119-Reverse air inlet structure, 120-Filter pipe, 121-Industrial camera, 101-Base frame, 102-Sealing ring, 2-Control module, 3-Light adjustment module, 4-Electrode adjustment module. Detailed Implementation

[0065] The technical solutions of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments; the components of this application described and shown in the accompanying drawings can be arranged and designed in various different configurations.

[0066] Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application; all other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0067] It should be noted that similar reference numerals and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures. At the same time, in the description of this application, the terms "first", "second", etc. are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0068] Existing cultivation techniques for *Tetranychus sylvestris* strains suffer from uneven lighting and imprecise environmental control, resulting in low purity of the cultured strains. To address these issues, this invention proposes a high-purity cultivation system for *Tetranychus sylvestris* strains (e.g.,...). Figure 2 As shown, it includes a culture device 1 and a control module 2. The culture device 1 includes a rectangular culture chamber 11 and an integrally formed equilateral triangular prism-shaped roof 12 on top of it.

[0069] A set of linear blue light strips 13 are respectively arranged on the three inner sides of the equilateral triangular prism-shaped ceiling 12. The first set extends along the top edge, and the second and third sets are symmetrically arranged along the upper edges of the two inclined surfaces. The three sets of light strips are spatially symmetrically distributed.

[0070] The center wavelength of each group of linear blue light strips 13 is 450±5nm;

[0071] The cultivation device 1 also integrates a light adjustment module 3, which is connected to the control module 2 and is configured to provide blue light irradiation that increases with time to the inoculation area of ​​the white rake tooth fungus strain during the cultivation process.

[0072] The gradient blue light irradiation gradually increases from 4–6 μmol / m² / s in the initial stage to 8–11 μmol / m² / s in the stable stage. For the *Bacillus thuringiensis* strain, the specific values ​​are: 5 ± 0.5 μmol / m² / s on day 1, 7 ± 0.5 μmol / m² / s on day 2, 9 ± 0.5 μmol / m² / s on day 3, and maintained at 10 ± 0.5 μmol / m² / s from day 4 to day 7.

[0073] The rectangular culture chamber 11 can be made of stainless steel to ensure its structural strength and corrosion resistance, and its size can be designed according to actual production needs;

[0074] The equilateral triangular prism-shaped ceiling 12 is integrally molded and can be manufactured using injection molding. The material can be a plastic with good light transmission.

[0075] The linear blue light strip 13 can use common LED light strips on the market. The center wavelength is controlled at 450±5nm because this wavelength range promotes the growth of white tooth bacteria.

[0076] The specific implementations mentioned above include: the lighting adjustment module 3 can be controlled by a microcontroller, and the gradient increase of blue light irradiance over time can be achieved through programming;

[0077] Throughout the cultivation system, the control module 2 controls the lighting adjustment module 3 according to a preset program, so that the linear blue light strip 13 provides blue light irradiation in a gradient manner. This lighting method can simulate the growth conditions of white tooth fungus in the natural environment and promote its growth.

[0078] This lighting method and culture system can improve the growth rate and purity of the white tooth fungus strain, make the strain grow more uniformly, and reduce the possibility of contamination by other microorganisms.

[0079] Among them, such as Figure 3 As shown, the inner wall of the rectangular culture chamber 11 is provided with symmetrically arranged slide rails 16 for slidably supporting the rectangular culture dish 19.

[0080] The rectangular petri dish 19 is slidably connected by a sliding plate 17 and a slide rail 16.

[0081] The rectangular culture dish 19 is also linked to the closed door 14 via a connecting rod 15, so that when the closed door 14 is pulled or pushed, the rectangular culture dish 19 is moved in and out of the rectangular culture chamber 11.

[0082] The slide rail 16 can be made of aluminum alloy with a smooth surface to reduce friction. The rectangular culture dish 19 can be made of glass or plastic, and its size is determined according to the culture requirements. The sliding plate 17 and the slide rail 16 must be fitted with high precision to ensure smooth sliding. The connecting rod 15 can be made of stainless steel and is connected to the rectangular culture dish 19 and the closed door 14 by welding or bolting.

[0083] The specific implementations mentioned above include: when the closed door 14 is pulled or pushed, the connecting rod 15 drives the rectangular culture dish 19 to slide along the slide rail 16, realizing the entry and exit of the culture dish. This linkage method makes the operation more convenient, reduces the contact between the operator and the culture environment, and improves the convenience of the culture dish entering and exiting. At the same time, it reduces the possibility of external pollution entering the culture room and ensures the stability of the culture environment.

[0084] Among them, such as Figure 2 As shown, an industrial camera 121 is provided on the side of the equilateral triangular prism-shaped canopy 12. The industrial camera 121 is connected to the control module 2 by signal, and its field of view completely covers the top surface of the rectangular culture dish 19, which is used to collect mycelial growth images in real time.

[0085] The industrial camera 121 is selected from models with high resolution and high frame rate to ensure that it can clearly capture images of mycelial growth and transmit data quickly. In practical applications, different models are selected according to conditions and costs, and this application embodiment does not limit this.

[0086] The specific implementation described above includes: the industrial camera 121 is connected to the control module 2 via a data cable, and transmits the acquired image data to the control module 2. The industrial camera 121 acquires real-time images of mycelial growth on the top surface of the rectangular culture dish 19 and transmits the image data to the control module 2. The control module 2 analyzes the image data to understand the mycelial growth status. By acquiring mycelial growth images in real time, problems that occur during mycelial growth, such as mycelial breakage and abnormal growth, can be detected in a timely manner so that timely measures can be taken to adjust the situation and improve the success rate of cultivation.

[0087] Among them, the light adjustment module 3 is configured to dynamically compensate and adjust the preset blue light irradiance intensity based on the mycelial coverage data collected by the industrial camera 121, and fluctuate within ±1.0 μmol / m² / s on the basic gradient value to adapt to the growth differences of different batches of fungi.

[0088] The light adjustment module 3 calculates the required blue light irradiance intensity based on the mycelial coverage data collected by the industrial camera 121 using an internal algorithm. If the mycelial coverage is low, it indicates that the mycelial growth is slow, and the light adjustment module 3 will appropriately increase the blue light irradiance intensity based on the basic gradient value. If the mycelial coverage is high, it indicates that the mycelial growth is fast, and the light adjustment module 3 will appropriately decrease the blue light irradiance intensity based on the basic gradient value. By dynamically compensating and adjusting the blue light irradiance intensity, different batches of mycelium can grow under suitable light conditions, improving the adaptability of the cultivation system, enhancing the growth quality and consistency of mycelium, and reducing growth differences between different batches of mycelium.

[0089] Among them, such as Figure 3 As shown, the rectangular culture dish 19 has four electrode plates 110 at the bottom. The four electrode plates 110 are evenly distributed around the central area and tilted towards the center at an angle of 15°±2°.

[0090] The culture device 1 also integrates an electrode adjustment module 4, which is connected to the control module 2 and is configured to apply a DC voltage of 1.2±0.05V to each electrode plate 110 to form a weak electric field on the surface of the culture medium to promote the directional growth of mycelia.

[0091] The electrode 110 can be made of a metal with good conductivity, such as copper or silver. The size and shape of the electrode 110 are designed according to the size of the rectangular culture dish 19. The electrode adjustment module 4 can be composed of a power supply module and a voltage regulation circuit, which applies a DC voltage of 1.2±0.05V to the electrode 110 through the command of the control module 2.

[0092] The specific implementations mentioned above include: after the electrode adjustment module 4 applies a DC voltage to the electrode plate 110, a weak electric field is formed on the surface of the culture medium. Under the action of the electric field, the hyphae will grow along the direction of the electric field. This method can achieve directional growth, that is, by forming a weak electric field to promote the directional growth of hyphae, the growth density and purity of hyphae can be improved, and the growth of the strain can be made more orderly.

[0093] Among them, such as Figure 4 , Figure 5 As shown, the bottom of the rectangular culture chamber 11 is provided with two rows of equally spaced through air inlets 114, which are located on both sides below the rectangular culture dish 19.

[0094] The through-type air inlet 114 is connected to the negative pressure fan 115 through the air inlet pipe 116. The negative pressure fan 115 introduces external air through the filter pipe 120 (the filter pipe 120 is equipped with a HEPA high-efficiency filter and an activated carbon layer, which is used to remove particulate matter and volatile organic compounds with a particle size ≥0.3μm in the air) and sends it into the air inlet pipe 116 after filtration.

[0095] The negative pressure fan 115 is connected to the control module 2 and is used to maintain the air pressure in the culture chamber at -40±2Pa. During the operation of the negative pressure fan 115, the airflow velocity is maintained between 0.15 and 0.25 m / s to achieve laminar airflow and avoid disturbing mycelial growth.

[0096] The rectangular culture chamber 11 has multiple spiral-shaped air outlet slots 118 arranged in a spiral path in the middle of its inner bottom;

[0097] The bottom center of the multi-loop air outlet 118 is provided with a reverse air intake structure 119, which is connected to the air outlet 119 to prevent unfiltered air from the outside from flowing back into the air outlet channel.

[0098] The reverse air intake structure 119 includes an inverted conical guide cavity and a one-way valve disposed at its bottom;

[0099] The upper end of the inverted conical guide cavity is connected to the multi-loop air outlet groove 118, and the lower part forms a closed exhaust channel;

[0100] The one-way valve is a spring-loaded duckbill valve or flap valve, configured to allow gas to be discharged from the culture chamber to the outside in only one direction. It automatically closes when the external pressure is higher than the internal pressure to prevent unfiltered air from flowing back in.

[0101] In the above, the diameter and spacing of the through-type air inlet 114 can be designed according to the size of the culture chamber and the ventilation requirements. The air inlet pipe 116 and the filter pipe 120 can be made of plastic or metal. The negative pressure fan 115 is selected with stable air pressure and air volume. Most importantly, it can ensure that the air pressure in the culture chamber is stable at −40±2Pa. The model of the negative pressure fan 115 is not limited in this embodiment. The multi-loop air outlet 118 can be made by injection molding and the material can be plastic. The reverse air inlet structure 119 can adopt a one-way valve or other structure.

[0102] The specific implementations mentioned above include: the negative pressure fan 115 filters the outside air through the filter pipe 120 and sends it into the culture chamber through the air inlet pipe 116 and the through-type air inlet 114. After the air flows in the culture chamber, it is discharged through the multi-turn air outlet slot 118. The reverse air inlet structure 119 prevents unfiltered outside air from flowing back into the air outlet channel and ensures air circulation in the culture chamber. At the same time, it prevents external pollutants from entering the culture chamber and improves the cleanliness of the culture environment.

[0103] The HEPA high-efficiency filter can be a commonly available model, offering high filtration efficiency. The activated carbon layer can be made of granular activated carbon, which has excellent adsorption properties. By controlling the speed of the negative pressure fan 115, the airflow velocity is maintained between 0.15 and 0.25 m / s. After entering the filter pipe 120, outside air first passes through the HEPA high-efficiency filter to remove particles with a diameter ≥0.3μm, and then passes through the activated carbon layer to remove volatile organic compounds. It then enters the cultivation chamber at a stable flow rate, achieving laminar flow air delivery and effectively removing airborne pollutants while avoiding disturbance to mycelial growth, thus ensuring the stability of the cultivation environment.

[0104] The inverted conical flow guide cavity can be made of plastic and formed by injection molding. The one-way valve can be a spring-loaded duckbill valve or a flap valve (in actual applications, the model of this component is selected according to different designs, and this application embodiment does not limit this).

[0105] When the gas in the culture chamber enters the inverted conical guide cavity through the multi-turn air outlet 118, the one-way valve opens and the gas is discharged. When the external pressure is higher than the internal pressure, the one-way valve automatically closes to prevent unfiltered air from flowing back in. This effectively prevents external air from flowing back into the air outlet channel and ensures the stability of the culture environment.

[0106] Among them, such as Figure 4 As shown, a number of sterilization lamps 117 are provided inside the rectangular culture chamber 11 and on both sides of the rectangular culture dish 19. The sterilization lamps 117 emit ultraviolet light or short-wave visible light and are used to periodically sterilize the area entering and exiting.

[0107] The germicidal lamp 117 is connected to the control module 2, and can be started, stopped, or have its irradiation parameters adjusted under control.

[0108] The germicidal lamp 117 includes a UVC ultraviolet lamp (wavelength 254±5 nm) and a purple LED (wavelength 405±10 nm), which can be turned on and off alternately or synchronously. The germicidal lamp 117 can be a UVC ultraviolet lamp or a purple LED lamp commonly available on the market. These two wavelengths of light have a good killing effect on bacteria and viruses.

[0109] The specific implementations mentioned above include: the germicidal lamp 117 is controlled by the control module 2, which can realize periodic start and stop and adjustment of irradiation parameters. The control module 2 controls the start and stop of the germicidal lamp 117 and the irradiation parameters according to the preset program. During the cultivation process, the germicidal lamp 117 performs periodic sterilization treatment on the entry and exit areas on both sides of the rectangular culture dish 19, and can effectively kill miscellaneous bacteria in the culture chamber, reduce the contamination of the cultured strains by miscellaneous bacteria, and improve the success rate of cultivation.

[0110] Among them, such as Figure 3As shown, a temperature sensor 111 and a humidity sensor 112 are provided in the middle of the rectangular culture dish 19. Both sensors are connected to the control module 2 for real-time monitoring of temperature and humidity changes in the culture environment. The temperature sensor 111 and the humidity sensor 112 can be common high-precision sensors on the market. This application embodiment does not limit the model of the two elements.

[0111] Specifically, the implementation involves: temperature sensor 111 and humidity sensor 112 collecting temperature and humidity data of the culture environment in real time and transmitting the data to control module 2; control module 2 analyzes and processes the temperature and humidity data to adjust the culture environment in a timely manner.

[0112] By monitoring changes in temperature and humidity in real time, abnormalities in the culture environment can be detected in a timely manner, and corresponding measures can be taken to make adjustments to ensure the stability of the culture environment.

[0113] Among them, such as Figure 3 As shown, the rectangular petri dish 19 has a spray structure 113 on its top;

[0114] The slide plate 17 is equipped with a solution injection device 18, which is connected to the spraying structure 113 through a pipeline and is used to spray sterile water or nutrient solution into the culture dish in a quantitative manner.

[0115] The solution injection device 18 is connected to the control module 2 and is controlled by the module to perform the spraying operation.

[0116] The solution injection device 18 is connected to two storage tanks, namely a sterile water storage tank and a nutrient solution storage tank, which are connected to the spraying structure 113 through an electromagnetic switching valve.

[0117] The control module 2 selectively triggers water replenishment (sterile water) or nutrient solution replenishment operations based on the combined data analysis results of the humidity sensor sensing element 112 and the temperature sensor sensing element 111.

[0118] Specifically, the following can be implemented: the spraying structure 113 can be a structure such as a nozzle, which can spray the solution evenly into the culture dish; the solution injection device 18 can be a device such as a peristaltic pump, which can realize quantitative injection; and the electromagnetic switching valve can select to deliver sterile water or nutrient solution to the spraying structure 113 according to the instructions of the control module 2.

[0119] Based on the combined data analysis results of the humidity sensor 112 and the temperature sensor 111, the control module 2 determines whether water or nutrient solution needs to be added. If so, the control module 2 controls the electromagnetic switching valve to select the appropriate storage tank and controls the solution injection device 18 to spray the solution into the culture dish through the spray structure 113.

[0120] The control module 2 is mounted on the body of the culture device 1 and is connected to an external computer via a communication interface.

[0121] like Figure 1 As shown, the control module 2 is configured to receive monitoring data from the industrial camera 121, the temperature sensor sensing element 111, the humidity sensor sensing element 112, and the dissolved oxygen detection device, and output corresponding control commands to the light adjustment module 3, the electrode adjustment module 4, the negative pressure fan 115, the germicidal lamp 117, and the solution injection device 18 to achieve closed-loop intelligent control.

[0122] Specifically, the control module 2 can be a microcontroller or industrial computer, which has strong computing and control capabilities. The communication interface can be a USB or Ethernet interface, which facilitates communication with an external computer. The control module 2 controls each module through programming.

[0123] Control module 2 receives monitoring data from various sensors and detection devices, analyzes and processes it, and outputs corresponding control commands to each module. Each module performs corresponding operations according to the control commands to achieve closed-loop intelligent control.

[0124] Among them, such as Figure 3 As shown, the outer edge of the closed door 14 is provided with a sealing ring 102 (usually made of silicone), which is used to seal the open side of the rectangular culture chamber 11 and to make the closed door 14 snap into the open side, so that the rectangular culture dish 19 can be fixed. This setting can improve the sealing performance between the closed door 14 and the culture chamber, prevent external pollutants from entering the culture chamber, ensure the stability of the culture environment, and also serve to fix the closed door 14.

[0125] The rectangular culture chamber 11 is provided with a base frame 101 at the bottom. The base frame 101 can be made of metal, such as steel, which has high strength and stability. The size and shape of the base frame 101 are designed according to the size of the rectangular culture chamber 11.

[0126] On the other hand, the present invention also provides a method for high-purity cultivation of *Tetranychus spp.* strains, implemented based on the cultivation system described in any of the above claims, comprising the following steps:

[0127] S1: Inoculate the white rake tooth bacterium strain onto the surface of the solid culture medium in a rectangular culture dish 19;

[0128] S2: Start control module 2 and control light adjustment module 3 to output blue light irradiation according to the preset program: 5±0.5μmol / m² / s on day 1, 7±0.5μmol / m² / s on day 2, 9±0.5μmol / m² / s on day 3, and 10±0.5μmol / m² / s from day 4 to day 7.

[0129] S3: Turn on the negative pressure fan 115 to supply air to the culture chamber through the filter pipe 120 and the air inlet pipe 116, and adjust the wind speed to maintain a negative pressure state of −40±2Pa in the chamber.

[0130] S4: The control electrode adjustment module 4 applies a DC voltage of 1.2±0.05V to the four tilted electrode plates 110 to establish a directional micro electric field in the culture medium area;

[0131] S5: Continuously acquire images of mycelial growth using industrial camera 121, and analyze mycelial coverage and breakage rate; simultaneously acquire environmental data through temperature sensor 111 and humidity sensor 112; and execute the following feedback control based on the monitoring results:

[0132] If the industrial camera 121 detects that the mycelial breakage rate exceeds 5% twice in a row, the light adjustment module 3 and the negative pressure fan 115 will be suspended for 1 hour before being resumed.

[0133] If the humidity is lower than the set threshold (the threshold can be adjusted according to the needs of the strain, and is not limited here), the solution injection device 18 is triggered to replenish sterile water or nutrient solution through the spray structure 113.

[0134] If the ratio of the outlet ultraviolet intensity to the inlet ultraviolet intensity of the germicidal lamp 117 is lower than 0.55±0.02, it indicates a decrease in sterilization efficiency, and the lamp will automatically increase its irradiation intensity or extend its operating time.

[0135] Adjust the negative pressure fan speed to 115 in real time to ensure that the pressure in the culture chamber is constant at −40±2Pa;

[0136] S6: After 7 days of cultivation, the sliding plate 17 and the rectangular culture dish 19 are moved out synchronously by pulling the closed door 14 to remove the culture product and complete the high-purity culture process.

[0137] In step S2, the control module 2 controls the light adjustment module 3 according to a preset program to achieve a gradient increase in blue light irradiance intensity; in step S3, the wind speed of the negative pressure fan 115 can be adjusted by a frequency converter or other equipment; in step S4, the electrode adjustment module 4 applies DC voltage to the electrode plate 110 through the power supply module; in step S5, the image data collected by the industrial camera 121 is analyzed by an image processing algorithm to obtain the mycelial coverage and breakage rate; in step S6, the closing door 14 should be pulled out slowly to avoid damaging the cultured product.

[0138] Throughout the cultivation process, control module 2 adjusts the cultivation environment in real time based on monitoring data from various sensors and detection devices; through feedback control, the stability and reliability of the cultivation process are ensured.

[0139] This cultivation method can improve the purity and quality of the *Bacillus thuringiensis* strain, achieve an intelligent cultivation process, reduce human intervention, and improve production efficiency.

[0140] This also includes the following intelligent control steps:

[0141] At fixed times each day, industrial camera 121 captures images of mycelium and calculates the daily mycelium coverage growth rate using a built-in algorithm; if the growth rate is less than 80% of the expected threshold, the cultivation time for days 4–7 is extended by 1–2 days.

[0142] After each solution spraying, wait 10 minutes before turning on the negative pressure fan 115 to prevent the droplets from being drawn away too quickly, which would result in uneven wetting.

[0143] After the culture is completed, control module 2 generates an electronic culture report containing temperature and humidity curves, light records, electric field application time, sterilization cycle and image change sequence, and uploads it to an external computer or cloud platform through the communication interface;

[0144] In the above, through these intelligent control steps, adjustments can be made according to the actual growth of mycelium, ensuring the cultivation effect. Delaying the start of the negative pressure fan 115 avoids the rapid removal of mist droplets, making the humidity in the culture dish more uniform. The electronic culture report facilitates the traceability and analysis of the cultivation process.

[0145] By adopting these intelligent control steps, the level of intelligence in the cultivation process is further improved, ensuring the quality and stability of the cultivation, while also facilitating the management and analysis of the cultivation process.

[0146] The above description is merely an embodiment of this application and is not intended to limit the scope of protection of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of protection of this application.

Claims

1. A high-purity culture system of Tricholoma lobayense strain, comprising a culture device (1) and a control module (2), characterized in that: the culture device (1) comprises a rectangular culture chamber (11) and an integral equilateral triangular prism-shaped ceiling (12) arranged on the top of the rectangular culture chamber (11); a set of linear blue light strips (13) is arranged on each of the three inner sides of the equilateral triangular prism-shaped ceiling (12), wherein the first set of linear blue light strips (13) is arranged along the top edge, and the second and third sets of linear blue light strips (13) are symmetrically arranged along the upper edges of the two inclined sides, and the three sets of linear blue light strips (13) are symmetrically distributed in space; the center wavelength of each set of linear blue light strips (13) is 450±5nm; the culture device (1) further integrates a light adjusting module (3), the light adjusting module (3) is connected with the control module (2) and is configured to provide a gradient-increasing blue light irradiation to the inoculation area of the Tricholoma lobayense strain over time during the culture process; the gradient-increasing blue light irradiation gradually increases from 4-6μmol / m² / s in the initial stage to 8-11μmol / m² / s in the stable stage. 2.The high-purity culture system of Tricholoma lobayense strain according to claim 1, characterized in that: a symmetrically arranged slide rail (16) is arranged on the inner side wall of the rectangular culture chamber (11) to slidably support a rectangular culture dish (19); the rectangular culture dish (19) is slidably connected with the slide rail (16) through a sliding plate (17); and the rectangular culture dish (19) is further connected with a sealing door (14) through a connecting rod (15) to realize linkage when the sealing door (14) is pulled or pushed, so that the rectangular culture dish (19) is brought into or out of the rectangular culture chamber (11). 3.The high-purity culture system of Tricholoma lobayense strain according to claim 1, characterized in that: an industrial camera (121) is arranged on the side of the equilateral triangular prism-shaped ceiling (12), the industrial camera (121) is signal-connected with the control module (2), and the shooting field of view of the industrial camera (121) completely covers the top surface of the rectangular culture dish (19) to realize real-time collection of mycelium growth images; four electrode sheets (110) are arranged at the bottom of the rectangular culture dish (19), the four electrode sheets (110) are uniformly distributed around the central region and are inclined to the center, and the inclination angle is 15°±2°; the culture device (1) further integrates an electrode adjusting module (4), the electrode adjusting module (4) is connected with the control module (2) and is configured to apply a direct current voltage of 1.2±0.05V to each electrode sheet (110) to form a weak electric field on the surface of the culture medium to promote directional growth of the mycelium; two rows of through-type air inlets (114) are arranged at the bottom of the rectangular culture chamber (11) and are arranged at equal intervals, the through-type air inlets (114) are located below the two sides of the rectangular culture dish (19); the through-type air inlets (114) are communicated with a negative pressure fan (115) through an air inlet pipeline (116), the negative pressure fan (115) introduces external air through a filter pipeline (120) and sends the filtered air into the air inlet pipeline (116). ​ ​ ​ ​ ​ ​ ​ ​ ​ 4. The system for high-purity culture of a Gomphidius strain according to claim 2, wherein: ​ ​ 5. The system for high-purity culture of a strain of the fungus Irpex lacteus according to claim 2, characterized in that: ​ ​ The negative pressure fan (115) is connected with the control module (2), and is used for maintaining the air pressure in the culture chamber at-40±2 Pa. A plurality of back-shaped air outlet grooves (118) arranged in a back-shaped path are arranged in the middle of the inner bottom of the rectangular culture chamber (11). A reverse air inlet structure (119) is arranged at the bottom of the back-shaped air outlet groove (118), and the reverse air inlet structure (119) is communicated with the air outlet groove, and is used for preventing the unfiltered air outside from flowing into the air outlet channel.

6. The system for high-purity culture of a Gomphidius strain according to claim 2, wherein: A plurality of sterilization light lamps (117) are arranged on both sides of the rectangular culture dish (19) in the rectangular culture chamber (11), the sterilization light lamps (117) emit ultraviolet light or short-wave visible light, and are used for periodically sterilizing the inlet and outlet areas. The sterilization light lamps (117) are connected with the control module (2) and are controlled to start and stop or adjust the irradiation parameters.

7. The system for high-purity culture of a Guepinomyces striatus strain according to claim 2, characterized in that: Temperature sensor sensing elements (111) and humidity sensor sensing elements (112) are arranged in the middle of the rectangular culture dish (19), and the two sensors are signal-connected with the control module (2) and are used for monitoring the temperature and humidity changes in the culture environment in real time.

8. The system for high-purity culture of a Guepinomyces striatus strain according to claim 2, characterized in that: The top of the rectangular culture dish (19) is provided with a spraying structure (113). A solution injection device (18) is arranged on the sliding plate (17), and the solution injection device (18) is connected with the spraying structure (113) through a pipeline and is used for quantitatively spraying sterile water or nutrient solution into the culture dish. The solution injection device (18) is connected with the control module (2) and is controlled to execute the spraying operation.

9. The high-purity culture system of a Guepinomyces strain according to any one of claims 1 to 8, characterized in that: The control module (2) is arranged on the body of the culture device (1) and is communicated with an external computer through a communication interface. The control module (2) is configured to receive monitoring data from the industrial camera (121), the temperature sensor sensing elements (111) and the humidity sensor sensing elements (112), and output corresponding control instructions to the light adjustment module (3), the electrode adjustment module (4), the negative pressure fan (115), the sterilization light lamps (117) and the solution injection device (18), so as to realize closed-loop intelligent regulation and control.

10. A method for high-purity culture of a strain of Skeletala albopilosa, implemented based on a high-purity culture system of a strain of Skeletala albopilosa according to any one of claims 1-9, characterized in that: The method comprises the following steps: S1: inoculate the white gill fungus strain on the surface of the solid culture medium in the rectangular culture dish (19); S2: start the control module (2), control the light adjustment module (3) to output blue light irradiation according to the preset program: 5±0.5 μmol / m² / s on the first day, 7±0.5 μmol / m² / s on the second day, 9±0.5 μmol / m² / s on the third day, and 10±0.5 μmol / m² / s on the fourth to seventh days; S3: start the negative pressure fan (115), supply air to the culture chamber through the filter pipeline (120) and the air inlet pipeline (116), and control the wind speed to maintain a negative pressure state of-40±2 Pa in the chamber; S4: control the electrode adjustment module (4) to apply a direct current voltage of 1.2±0.05 V to the four pieces of inclined electrode sheets (110) to establish a directional micro-electric field in the culture medium area. S5: Continuously collect mycelium growth images using an industrial camera (121), analyze mycelium coverage and breakage rate; at the same time, obtain environmental data through temperature sensor sensing element (111) and humidity sensor sensing element (112); according to the monitoring results, the following feedback control is executed: If the industrial camera identifies that the mycelium breakage rate exceeds 5% for two consecutive times, the light adjustment module (3) and the negative pressure fan (115) are suspended for 1 hour before resuming; If the humidity is lower than the set threshold, the solution injection device (18) is triggered to supplement sterile water or nutrient solution through the spraying structure (113); If the outlet ultraviolet intensity of the sterilization light lamp (117) is less than 0.55±0.02 compared with the inlet, indicating that the sterilization efficiency is decreased, the irradiation intensity is automatically increased or the opening time is prolonged; Real-time adjustment of the speed of the negative pressure fan (115) to ensure that the pressure in the culture chamber is constant at−40±2Pa; S6: After 7 days of culture, the closed door (14) is pulled to drive the slide plate (17) and the rectangular culture dish (19) to move out synchronously, the culture product is taken out, and the high-purity culture process is completed.

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