Preparation method of NAD amino acid nutrient solution wrapped by nano-liposome

Abstract

The invention discloses a preparation method of a nano-liposome coated NAD amino acid nutrient solution, and relates to the technical field of liposome amino acid nutrient solution preparation, the preparation method comprises the following steps: S1, preparing raw materials: S1.1, preparing NAD amino acid; s1.2, lecithin: using pure natural lecithin from soybeans and eggs; s1.3, preparing sodium hyaluronate: adopting sodium hyaluronate with low molecular weight; s1.4, preparing an amino acid compound, wherein the amino acid compound contains a plurality of essential amino acids, including glycine, serine and lysine; according to the mask, when the NAD amino acid nutrient solution wrapped by the nano-liposome is applied to the mask, the mask can more effectively permeate into the deep layer of the skin due to the excellent stability and biological activity of the NAD amino acid nutrient solution, and sufficient nutrition and moisture are provided for the skin; meanwhile, due to the strong anti-aging effect, the mask can remarkably improve the skin texture, reduce fine wrinkles and wrinkles, and make the skin more compact, smoother and elastic.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of liposome amino acid nutrient solution preparation, in particular to a preparation method of NAD amino acid nutrient solution wrapped by nanoliposomes. BACKGROUND

[0002] In the field of cosmetics and skincare, the stability and bioavailability of active ingredients have always been the focus of research and development. In traditional skincare products, active ingredients are often directly added to the formula, but due to the presence of the skin barrier, these active ingredients often have difficulty penetrating effectively into the deep layers of the skin, thereby limiting their skincare effects. In addition, some active ingredients have poor stability in skincare products and are easily affected by external environmental factors such as temperature, light, etc., leading to product deterioration, reducing the product's use effect and safety. In order to solve these problems, researchers are constantly exploring new technologies to improve the stability and bioavailability of active ingredients. Among them, nanoliposome technology as an effective drug and active ingredient delivery system has received widespread attention. Nanoliposomes have a unique bilayer membrane structure that can encapsulate and protect active ingredients, preventing them from being damaged by the external environment, while improving their penetration and stability in the skin.

[0003] However, existing nanoliposome technology still has some shortcomings. On the one hand, the preparation process of nanoliposomes is complex and requires precise control of various parameters such as temperature, stirring speed, ultrasonic power, etc. to ensure that active ingredients are uniformly encapsulated. On the other hand, traditional nanoliposome technology often uses chemically synthesized lecithin or other synthetic materials in the preparation process, which may have safety issues and are not easily absorbed and utilized by the skin.

[0004] In view of this, a preparation method of NAD amino acid nutrient solution wrapped by nanoliposomes is provided to overcome the above problems, to improve the stability and bioavailability of active ingredients, while ensuring the safety and effectiveness of the product, which has important research significance and application value. This technology is proposed in this background, by optimizing raw material selection, preparation process and formula design, a NAD amino acid nutrient solution wrapped by nanoliposomes with good stability and high bioavailability is successfully prepared, providing a new idea and method for the development of the field of cosmetics and skincare. SUMMARY

[0005] The purpose of the present application is to provide a preparation method of NAD amino acid nutrient solution wrapped by nanoliposomes to solve the problems raised in the background art.

[0006] To solve the above technical problems, the present application provides a preparation method of NAD amino acid nutrient solution wrapped by nanoliposomes, comprising:

[0007] S1, Preparation of raw materials:

[0008] S1.1, NAD amino acid;

[0009] S1.2, lecithin: use of pure natural lecithin from soybeans, eggs;

[0010] S1.3, sodium hyaluronate: use of low molecular weight sodium hyaluronate;

[0011] S1.4, amino acid complex: contains multiple essential amino acids, including glycine, serine and lysine;

[0012] S1.5, high-purity nicotinamide;

[0013] S1.6, ascorbic acid;

[0014] S1.7, plant extract: combined with purslane extract, golden chamomile extract;

[0015] S2, preparation of nutrient solution:

[0016] S2.1, preparation of liposome solution: mix lecithin with ethanol, heat to 50-55°C, stir until phospholipid is completely dissolved, get uniform liposome solution;

[0017] S2.2, mixing of active ingredients: mix lecithin, NAD amino acid, sodium hyaluronate, amino acid complex, nicotinamide, ascorbic acid, plant extract, according to the ratio of 10:5:3:2:1:0.5:1, heat to 30-35°C, stir until uniform, form active ingredient solution;

[0018] S2.3, liposome wrapping: slowly drop the active ingredient solution into the liposome solution through the precise liquid device, start the ultrasonic disperser at the same time, ultrasonic power 100W, under the joint action of stirring and ultrasonic, make the active ingredient be uniformly wrapped by liposome;

[0019] S2.4, removal of solvent: transfer the obtained mixed solution to the rotary evaporator, set the temperature to 40°C and the vacuum degree to-0.08MPa, accurately control the evaporation speed of the solvent, ensure the stability of the active ingredients and liposomes;

[0020] S2.5, product modulation: add glycerol, potassium sorbate, and adjust the pH value to 5.5-6.5.

[0021] Further, in S1.1, the amino acid selects NAD+ precursor with purity higher than 98%, including NMN nicotinamide mononucleotide.

[0022] Further, in S1.2, the lecithin selects HSPC hydrogenated lecithin.

[0023] Further, in S1.6, ascorbic acid is selected to esterify vitamin C.

[0024] Further, in S2.1, HSPC is mixed with ethanol at an optimized ratio, heated to 52°C, and stirred at a stirring speed of 300 rpm until the phospholipid is completely dissolved.

[0025] Further, in S2.2, HSPC, NMN, sodium hyaluronate, and amino acid complex are first added, then nicotinamide and ascorbic acid are gradually added, and finally plant extract is added, maintaining 35°C and a stirring speed of 400 rpm throughout the process to form an active ingredient solution; the mixture is mixed in a ratio of 10:5:3:2:1:0.5:1.

[0026] Further, in S2.3, using a high-frequency ultrasonic disperser, set the power to 120W, the frequency to 20kHz, and the processing time to 30 minutes, slowly drop the active ingredient solution into the liposome solution while turning on the stirrer.

[0027] Further, in S2.4, the mixed solution is transferred to a rotary evaporator, set the temperature to 42°C and the vacuum degree to -0.085MPa, until the solvent is completely evaporated.

[0028] Compared with the prior art, the beneficial effects of the present application are:

[0029] Example eight is the optimal embodiment, which improves the stability and bioavailability of the product, makes the active ingredients more effectively absorbed and utilized by the skin, enhances the skin care effect of the product such as improving skin aging, moisturizing, etc., improves the market competitiveness of the product, and meets the needs of consumers for high-quality skin care products.

[0030] When the nanoliposome-encapsulated NAD amino acid nutrient solution prepared in Example eight is applied to a facial mask, its superior stability and biological activity enable the facial mask to penetrate more effectively into the deep layers of the skin, providing sufficient nutrients and moisture to the skin; at the same time, due to its strong anti-aging effect, the facial mask can significantly improve skin texture, reduce fine lines and wrinkles, and make the skin more firm, smooth and elastic. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 The flowchart of the preparation method of the nanoliposome-encapsulated NAD amino acid nutrient solution of the present application. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of the present application.

[0033] Referring to Figure 1 The present application provides a technical solution:

[0034] Referring to Figure 1 A preparation method of a nanoliposome-encapsulated NAD amino acid nutrient solution is shown.

[0035] Embodiment one:

[0036] S1, preparation of raw materials:

[0037] S1.1, NAD amino acid;

[0038] S1.2, lecithin: pure natural lecithin from chicken eggs is used to improve the stability and biocompatibility of the liposome;

[0039] S1.3, sodium hyaluronate: low molecular weight (1000-1500 Da) sodium hyaluronate is used to achieve stronger moisturizing effect;

[0040] S1.4, amino acid complex: contains various essential amino acids, including glycine, serine and lysine, to enhance skin repair and cell vitality;

[0041] S1.5, high-purity nicotinamide; to ensure its whitening and anti-aging effect;

[0042] S1.6, ascorbic acid;

[0043] S1.7, plant extract: combined with spilanthes extract, golden chamomile extract;

[0044] S2, preparation of nutrient solution:

[0045] S2.1, preparation of liposome solution: mix lecithin with ethanol, heat to 50-55℃, stir until the phospholipid is completely dissolved, and obtain a uniform liposome solution;

[0046] S2.2, mixing of active ingredients: mix lecithin, NAD amino acid, sodium hyaluronate, amino acid complex, nicotinamide, ascorbic acid, plant extract, in the ratio of 10:5:3:2:1:0.5:1, heat to 30-35℃, stir until uniform, form active ingredient solution

[0047] S2.3, Liposome Encapsulation: The active ingredient solution is slowly dropped into the liposome solution through a precise droplet device while starting the ultrasonic disperser with a power of 100W. Under the combined action of stirring and ultrasonic, the active ingredient is uniformly encapsulated by the liposome;

[0048] S2.4, Solvent Removal: The resulting mixed solution is transferred to a rotary evaporator with a temperature setting of 40°C and a vacuum degree of -0.08MPa. The evaporation speed of the solvent is precisely controlled to ensure the stability of the active ingredient and the liposome;

[0049] S2.5, Product Modulation: Glycerol and potassium sorbate are added, and the pH value is adjusted to 5.5-6.5.

[0050] Example Two:

[0051] S1, Preparation of Raw Materials:

[0052] S1.1, NAD Amino Acid: Select NAD+ precursor with purity higher than 98%, including NMN nicotinamide mononucleotide, to ensure high activity and good skin penetration;

[0053] S1.2, Egg Phospholipid: Use pure natural egg phospholipid from chicken eggs to improve the stability and biocompatibility of the liposome;

[0054] S1.3, Sodium Hyaluronate: Use low molecular weight (1000-1500Da) sodium hyaluronate to achieve stronger moisturizing effect;

[0055] S1.4, Amino Acid Complex: Contains multiple essential amino acids, including glycine, serine and lysine, to enhance skin repair and cell vitality;

[0056] S1.5, High Purity Nicotinamide: To ensure its whitening and anti-aging effect;

[0057] S1.6, Ascorbic Acid;

[0058] S1.7, Plant Extracts: Combine with purslane extract and golden chamomile extract;

[0059] S2, Preparation of Nutrient Solution:

[0060] S2.1, Preparation of Liposome Solution: Mix egg phospholipid with ethanol, heat to 50-55°C, and stir until the phospholipid is completely dissolved to obtain a uniform liposome solution;

[0061] S2.2, Active Ingredient Mixing: Mix egg phospholipid, NAD amino acid, sodium hyaluronate, amino acid complex, nicotinamide, ascorbic acid, and plant extract in a ratio of 10:5:3:2:1:0.5:1. Heat to 30-35°C and stir until uniform to form an active ingredient solution;

[0062] S2.3, Liposome wrapping: The active ingredient solution is slowly dropped into the liposome solution through a precise droplet device, while starting the ultrasonic disperser with a power of 100W. Under the combined action of stirring and ultrasonic, the active ingredient is uniformly wrapped by the liposome;

[0063] S2.4, Solvent removal: The obtained mixed solution is transferred to a rotary evaporator, with a temperature setting of 40°C and a vacuum degree of -0.08MPa. The evaporation speed of the solvent is precisely controlled to ensure the stability of the active ingredient and the liposome;

[0064] S2.5, Product modulation: Add glycerol and potassium sorbate, and adjust the pH value to 5.5-6.5.

[0065] Example Three:

[0066] S1, Preparation of raw materials:

[0067] S1.1, NAD amino acid: Select NAD+ precursor with purity higher than 98%, including NMN nicotinamide mononucleotide, to ensure high activity and good skin penetration;

[0068] S1.2, Egg phospholipid: Select HSPC hydrogenated egg phospholipid because it has higher stability and biocompatibility;

[0069] S1.3, Sodium hyaluronate: Use low molecular weight (1000-1500Da) sodium hyaluronate to achieve stronger moisturizing effect;

[0070] S1.4, Amino acid complex: Contains multiple essential amino acids, including glycine, serine and lysine, to enhance skin repair and cell vitality;

[0071] S1.5, High-purity nicotinamide; to ensure its whitening and anti-aging effect;

[0072] S1.6, Ascorbic acid;

[0073] S1.7, Plant extract: Combine with spilanthes extract and golden chamomile extract;

[0074] S2, Preparation of nutrient solution:

[0075] S2.1, Preparation of liposome solution: Mix egg phospholipid with ethanol, heat to 50-55°C, and stir until the phospholipid is completely dissolved to obtain a uniform liposome solution;

[0076] S2.2, Active ingredient mixing: Mix lecithin, NAD amino acid, sodium hyaluronate, amino acid complex, nicotinamide, ascorbic acid, plant extract in the ratio of 10:5:3:2:1:0.5:1, heat to 30-35°C, stir until uniform, form active ingredient solution;

[0077] S2.3, Liposome wrapping: Slowly drop the active ingredient solution into the liposome solution through the precise dropper, start the ultrasonic disperser at the same time, ultrasonic power 100W, under the joint action of stirring and ultrasonic, make the active ingredient be uniformly wrapped by liposome;

[0078] S2.4, Solvent removal: Transfer the obtained mixed solution to the rotary evaporator, set the temperature (40°C) and vacuum degree (-0.08MPa), precisely control the evaporation speed of the solvent, ensure the stability of the active ingredient and liposome;

[0079] S2.5, Product modulation: Add glycerol, potassium sorbate, and adjust the pH value to 5.5-6.5.

[0080] Example four:

[0081] S1, Preparation of raw materials:

[0082] S1.1, NAD amino acid: Select NAD+ precursor with purity higher than 98%, including NMN nicotinamide mononucleotide, to ensure high activity and good skin permeability;

[0083] S1.2, Lecithin: Choose HSPC hydrogenated lecithin because it has higher stability and biocompatibility;

[0084] S1.3, Sodium hyaluronate: Use low molecular weight (1000-1500Da) sodium hyaluronate to obtain stronger moisturizing effect;

[0085] S1.4, Amino acid complex: Contains multiple essential amino acids, including glycine, serine and lysine, to enhance skin repair and cell vitality;

[0086] S1.5, High-purity nicotinamide; to ensure its whitening and anti-aging effect;

[0087] S1.6, Ascorbic acid;

[0088] S1.7, Plant extract: Combine with purslane extract and golden chamomile extract;

[0089] S2, Preparation of nutrient solution:

[0090] S2.1, Preparation of liposome solution: Mix HSPC with ethanol at the optimized ratio, heat to 52°C, and stir at a stirring speed of 300 rpm until the phospholipid is completely dissolved;

[0091] S2.2, Active ingredient mixing: Mix lecithin, NAD amino acid, sodium hyaluronate, amino acid complex, nicotinamide, ascorbic acid, and plant extract at a ratio of 10:5:3:2:1:0.5:1, heat to 30-35°C, and stir until uniform to form an active ingredient solution;

[0092] S2.3, Liposome encapsulation: Slowly drop the active ingredient solution into the liposome solution through a precise dropper, and start the ultrasonic disperser at a power of 100 W. Under the combined action of stirring and ultrasonic waves, the active ingredients are uniformly encapsulated by the liposomes;

[0093] S2.4, Solvent removal: Transfer the resulting mixed solution to a rotary evaporator, set the temperature (40°C) and vacuum degree (-0.08 MPa), and precisely control the evaporation speed of the solvent to ensure the stability of the active ingredients and liposomes;

[0094] S2.5, Product conditioning: Add glycerol and potassium sorbate, and adjust the pH to 5.5-6.5.

[0095] Example Five:

[0096] S1, Preparation of raw materials:

[0097] S1.1, NAD amino acid: Select NAD+ precursors with a purity higher than 98%, including NMN nicotinamide mononucleotide, to ensure high activity and good skin penetration;

[0098] S1.2, Lecithin: Choose HSPC hydrogenated lecithin, as it has higher stability and biocompatibility;

[0099] S1.3, Sodium hyaluronate: Use low molecular weight (1000-1500 Da) sodium hyaluronate to achieve stronger moisturizing effect;

[0100] S1.4, Amino acid complex: Contains multiple essential amino acids, including glycine, serine, and lysine, to enhance skin repair and cell vitality;

[0101] S1.5, High-purity nicotinamide; to ensure its whitening and anti-aging effect;

[0102] S1.6, Ascorbic acid: Choose esterified vitamin C;

[0103] S1.7, Plant extract: Combine with purslane extract and golden chamomile extract;

[0104] S2, Preparation of nutrient solution:

[0105] S2.1, Preparation of liposome solution: Mix HSPC with ethanol at an optimized ratio, heat to 52°C, and stir at a stirring speed of 300 rpm until the phospholipid is completely dissolved;

[0106] S2.2, Active ingredient mixing: Mix lecithin, NAD amino acid, sodium hyaluronate, amino acid complex, nicotinamide, ascorbic acid, and plant extract at a ratio of 10:5:3:2:1:0.5:1, heat to 30-35°C, and stir until uniform to form an active ingredient solution;

[0107] S2.3, Liposome encapsulation: Slowly drop the active ingredient solution into the liposome solution through a precise dropper, and start the ultrasonic disperser at a power of 100W. Under the combined action of stirring and ultrasonic waves, the active ingredients are uniformly encapsulated by the liposomes;

[0108] S2.4, Solvent removal: Transfer the resulting mixed solution to a rotary evaporator, set the temperature (40°C) and vacuum degree (-0.08MPa), and precisely control the evaporation speed of the solvent to ensure the stability of the active ingredients and liposomes;

[0109] S2.5, Product modulation: Add glycerol and potassium sorbate, and adjust the pH value to 5.5-6.5.

[0110] Example six:

[0111] S1, Preparation of raw materials:

[0112] S1.1, NAD amino acid: Select NAD+ precursors with a purity higher than 98%, including NMN nicotinamide mononucleotide, to ensure high activity and good skin penetration;

[0113] S1.2, Lecithin: Select HSPC hydrogenated lecithin, as it has higher stability and biocompatibility;

[0114] S1.3, Sodium hyaluronate: Use low molecular weight (1000-1500Da) sodium hyaluronate to achieve stronger moisturizing effect;

[0115] S1.4, Amino acid complex: Contains multiple essential amino acids, including glycine, serine and lysine, to enhance skin repair and cell vitality;

[0116] S1.5, High-purity nicotinamide; to ensure its whitening and anti-aging effect;

[0117] S1.6, Ascorbic acid: Select esterified vitamin C;

[0118] S1.7, Plant Extracts: Combination of Purslane Extract, Golden Yarrow Extract;

[0119] S2, Preparation of Nutrient Solution:

[0120] S2.1, Preparation of Liposome Solution: Mix HSPC with ethanol at an optimized ratio, heat to 52°C, and stir at a stirring speed of 300 rpm until the phospholipids are completely dissolved;

[0121] S2.2, Active Ingredient Mixing: First, add HSPC, NMN, sodium hyaluronate, and amino acid complex, then gradually add nicotinamide and ascorbic acid, and finally add plant extracts, maintaining a stirring speed of 35°C and 400 rpm throughout the process, forming an active ingredient solution; during the process, mix at a ratio of 10:5:3:2:1:0.5:1;

[0122] S2.3, Liposome Encapsulation: Slowly drip the active ingredient solution into the liposome solution through a precise droplet device, while starting the ultrasonic disperser at a power of 100W, under the combined action of stirring and ultrasonic waves, so that the active ingredients are uniformly encapsulated by the liposomes;

[0123] S2.4, Solvent Removal: Transfer the resulting mixed solution to a rotary evaporator, set the temperature (40°C) and vacuum degree (-0.08MPa), and precisely control the evaporation speed of the solvent to ensure the stability of the active ingredients and liposomes;

[0124] S2.5, Product Modulation: Add glycerol and potassium sorbate, and adjust the pH value to 5.5-6.5.

[0125] Example Seven:

[0126] S1, Preparation of Raw Materials:

[0127] S1.1, NAD Amino Acids: Select NAD+ precursors with a purity higher than 98%, including NMN nicotinamide mononucleotide, to ensure high activity and good skin penetration;

[0128] S1.2, Egg Phospholipids: Choose HSPC hydrogenated egg phospholipids because they have higher stability and biocompatibility;

[0129] S1.3, Sodium Hyaluronate: Use low molecular weight (1000-1500Da) sodium hyaluronate to achieve stronger moisturizing effect;

[0130] S1.4, Amino Acid Complex: Contains multiple essential amino acids, including glycine, serine, and lysine, to enhance skin repair and cell vitality;

[0131] S1.5, High-purity Nicotinamide; to ensure its whitening and anti-aging effect;

[0132] S1.6, Ascorbic Acid: Select esterified Vitamin C;

[0133] S1.7, Plant Extracts: Combine with Spinach Extract, Golden Yarrow Extract;

[0134] S2, Preparation of Nutrient Solution:

[0135] S2.1, Preparation of Liposome Solution: Mix HSPC with ethanol at optimized ratio, heat to 52°C, and stir at 300 rpm until phospholipids are completely dissolved;

[0136] S2.2, Active Ingredient Mixing: First add HSPC, NMN, Sodium Hyaluronate, and Amino Acid Complex, then gradually add Nicotinamide and Ascorbic Acid, and finally add Plant Extracts, maintaining 35°C and 400 rpm stirring throughout the process, forming an active ingredient solution; mix at a ratio of 10:5:3:2:1:0.5:1;

[0137] S2.3, Liposome Encapsulation: Use a high-frequency ultrasonic disperser, set power to 120W, frequency to 20kHz, and processing time to 30 minutes, slowly drop the active ingredient solution into the liposome solution while turning on the stirrer;

[0138] S2.4, Solvent Removal: Transfer the resulting mixed solution to a rotary evaporator, set temperature (40°C) and vacuum degree (-0.08MPa), precisely control the evaporation speed of the solvent to ensure the stability of the active ingredients and liposomes;

[0139] S2.5, Product Modulation: Add glycerol and potassium sorbate, and adjust pH to 5.5-6.5.

[0140] Example Eight:

[0141] S1, Preparation of Raw Materials:

[0142] S1.1, NAD Amino Acids: Select NAD+ precursors with purity higher than 98%, including NMN Nicotinamide Mononucleotide, to ensure high activity and good skin penetration;

[0143] S1.2, Egg Phospholipids: Choose HSPC hydrogenated egg phospholipids for higher stability and biocompatibility;

[0144] S1.3, Sodium Hyaluronate: Use low molecular weight (1000-1500Da) sodium hyaluronate for stronger moisturizing effect;

[0145] S1.4, Amino Acid Complex: Contains multiple essential amino acids, including glycine, serine, and lysine, to enhance skin repair and cell vitality;

[0146] S1.5, high-purity nicotinamide; to ensure its whitening and anti-aging effects;

[0147] S1.6, ascorbic acid: select esterified vitamin C;

[0148] S1.7, plant extract: combine with purslane extract and golden chamomile extract;

[0149] S2, preparation of nutrient solution:

[0150] S2.1, preparation of liposome solution: mix HSPC with ethanol at the optimized ratio, heat to 52°C, and stir at a stirring speed of 300 rpm until the phospholipid is completely dissolved;

[0151] S2.2, mixing of active ingredients: first add HSPC, NMN, sodium hyaluronate, and amino acid complex, then gradually add nicotinamide and ascorbic acid, and finally add plant extract, maintaining a stirring speed of 35°C and 400 rpm throughout the process, to form an active ingredient solution; during the process, mix at a ratio of 10:5:3:2:1:0.5:1;

[0152] S2.3, liposome encapsulation: use a high-frequency ultrasonic disperser, set the power to 120W, the frequency to 20kHz, and the processing time to 30 minutes, slowly drop the active ingredient solution into the liposome solution, and turn on the stirrer at the same time;

[0153] S2.4, solvent removal: transfer the mixed solution to a rotary evaporator, set the temperature to 42°C and the vacuum degree to -0.085MPa, until the solvent is completely evaporated;

[0154] S2.5, product conditioning: add glycerol and potassium sorbate, and adjust the pH value to 5.5-6.5.

[0155] Effect analysis of Example One to Example Eight:

[0156] Example One (basic formula):

[0157] Effect: a basic NAD amino acid nutrient solution encapsulated by nanoliposomes is obtained, but the stability and biological activity are relatively general;

[0158] Example Two (optimization of NAD amino acid purity):

[0159] Effect: due to the use of high-purity NMN, the anti-aging effect of the product is significantly improved;

[0160] Example Three (optimization of lecithin source):

[0161] Effect: the use of HSPC hydrogenated lecithin enhances the stability and encapsulation efficiency of the liposomes;

[0162] Example Four (Optimizing Ascorbic Acid Form):

[0163] Effect: Esterified Vitamin C improves the stability and bioavailability of ascorbic acid;

[0164] Example Five (Optimizing Liposome Solution Preparation Conditions):

[0165] Effect: By precisely controlling temperature and stirring speed, the uniformity and stability of the liposome solution are improved;

[0166] Example Six (Optimizing Active Ingredient Mixing Method):

[0167] Effect: By gradually adding raw materials and maintaining constant temperature and stirring speed, uniform mixing and stability of active ingredients are ensured;

[0168] Example Seven (Optimizing Liposome Encapsulation Conditions):

[0169] Effect: Using a high-frequency ultrasonic disperser optimizes the encapsulation process, improving encapsulation efficiency and product stability;

[0170] Example Eight (Optimal Formula and Process) (Optimal Example):

[0171] Effect: By integrating all optimization measures, the best stability, highest bioactivity, and strongest anti-aging effect of nanoliposome-encapsulated NAD amino acid nutrient solution are achieved;

[0172] Effect:

[0173] Stability: By optimizing raw material selection and preparation process, the stability of the product is significantly improved, and the shelf life is extended;

[0174] Bioactivity: Using high-purity raw materials and optimized preparation conditions ensures the bioactivity and effectiveness of the product;

[0175] Anti-aging effect: Especially Example Eight, through comprehensive optimization, the anti-aging effect of the product is greatly improved;

[0176] Application of Example Eight in Facial Masks:

[0177] When the nanoliposome-encapsulated NAD amino acid nutrient solution prepared by Example Eight is applied in facial masks, its superior stability and bioactivity enable the mask to penetrate more effectively into the deep layers of the skin, providing sufficient nutrition and moisture to the skin; at the same time, due to its strong anti-aging effect, the mask can significantly improve skin texture, reduce fine lines and wrinkles, making the skin more firm, smooth, and elastic;

[0178] Therefore, Example Eight is the optimal embodiment, which improves the stability and bioavailability of the product, makes the active ingredients more effectively absorbed and utilized by the skin, enhances the skin care effect of the product, such as improving skin aging, moisturizing, etc., improves the market competitiveness of the product, and meets the needs of consumers for high-quality skin care products.

[0179] Summary:

[0180] Liposome cosmetics refer to a type of cosmetics that encapsulate active ingredients in a phospholipid bilayer using liposome technology. Liposomes are spherical vesicles formed by a phospholipid bilayer similar to cell membranes, which can effectively encapsulate active ingredients. The main advantages include: improving the stability of active ingredients: liposomes can protect active ingredients from external environmental factors (such as light, air, oxygen, etc.), thereby extending their effective period.

[0181] Enhanced permeability: due to the structure similar to the skin cell membrane, liposomes can more easily penetrate the skin barrier, delivering active ingredients to the deep layers of the skin.

[0182] Sustained-release effect: liposomes can control the release rate of active ingredients, allowing them to continuously exert their effects on the skin, thereby improving the efficacy of cosmetics.

[0183] Reduced irritation: encapsulation by liposomes can reduce the irritation of certain active ingredients to the skin, making them suitable for sensitive skin care products.

[0184] Egg phospholipids:

[0185] Skin moisture retention: due to the hydrophilic and lubricating properties of egg phospholipids, they can help the stratum corneum retain moisture, increasing the water content of the skin.

[0186] Skin barrier repair: egg phospholipids can promote cell metabolism and enhance the connection between epidermal cells, thereby repairing the skin barrier.

[0187] Anti-aging: the unsaturated fatty acids in egg phospholipids can inhibit the increase of cholesterol content in the body, reduce blood lipid levels, and delay the occurrence and development of atherosclerosis. At the same time, they can also promote blood circulation and accelerate cell turnover, making the skin smoother and more elastic.

[0188] Whitening;

[0189] Improving skin quality: egg phospholipids can improve oily skin by regulating the secretion function of sebaceous glands, and also promote the formation of the stratum corneum, making dry skin more moisturizing.

[0190] Sodium hyaluronate: can form a layer of moisturizing film on the skin or mucous membrane surface, effectively lock in moisture, increase the local water content. Therefore, sodium hyaluronate is often used for eye moisturizing. Sodium hyaluronate has a lubricating effect, which can fill the gap between tissues. Therefore, for dry eyes, sodium hyaluronate has a good improvement effect.

[0191] Sodium hyaluronate has good biocompatibility and safety, can promote human immune function, and enhance the body's resistance to disease.

[0192] Sodium hyaluronate has a certain repair effect on skin damage, can promote skin cell growth and wound healing, and restore the damaged site to health.

[0193] Amino acids have moisturizing, repairing, antioxidant and other effects on the skin. Amino acids are the basic units of protein, and have good moisturizing effect, which can provide sufficient moisture to the skin. They can absorb and lock in moisture, maintain the moisture balance of the skin, making the skin softer and more elastic; they can also promote the repair of skin tissue and help the skin recover.

[0194] Appropriate use of amino acids can help speed up skin cell metabolism and promote skin cell regeneration, thereby reducing the time and range of skin damage; they also have antioxidant effects, which can resist free radical damage and slow down the rate of skin aging.

[0195] They can neutralize free radicals and help repair skin tissue damaged by oxidation, making the skin younger and more radiant.

[0196] In addition, amino acids have activity and can stimulate skin cell vitality, helping the skin promote metabolism and increase skin radiance.

[0197] Nicotinamide: can promote skin metabolism, help skin toxins and garbage discharge, and also improve skin dullness. Nicotinamide can promote blood circulation in the skin, also has the effect of shrinking pores, and can improve the acne marks left after acne elimination.

[0198] Nicotinamide can play an antioxidant role, which can remove free radicals in the skin, is beneficial to the health of the skin, and can also delay skin aging.

[0199] Nicotinamide can promote skin metabolism and also has an anti-aging effect, inhibit the synthesis of melanocytes, help prevent melanin deposition, and also improve the appearance of skin discoloration.

[0200] Purslane extract: It moisturizes and lubricates the skin, effectively removing dead skin cells and excess keratin, resulting in smoother, more refined skin. Rich in Vitamin E and Vitamin C, purslane offers excellent skincare benefits, primarily by helping to eliminate free radicals and stimulating cell growth and metabolism, thus improving skin condition. Furthermore, due to its antibacterial and anti-inflammatory properties, using skincare products containing purslane can improve the skin's barrier function and reduce external irritation.

[0201] Golden chamomile extract: Contains flavonoids, an active ingredient that acts as an antioxidant and can scavenge free radicals. Appropriate use of skincare products containing golden chamomile extract can, to some extent, prevent skin aging.

[0202] Repairing sensitive skin: Golden chamomile extract has a cooling effect and a good soothing effect, which can improve sensitive, dehydrated, dry and peeling skin, making it suitable for people with sensitive skin.

[0203] Reduce redness: Golden chamomile extract has an anti-inflammatory effect, can inhibit capillary permeability, can soothe skin microvessels, and reduce redness on the face, making it suitable for people with redness.

[0204] Ascorbic acid (vitamin C): It has certain antioxidant effects on the skin and can help the skin resist external factors.

[0205] Environmental damage can also help the skin retain moisture. Ascorbic acid is a water-soluble vitamin and an essential vitamin for the human body. It helps the body resist damage from the external environment and retain moisture. A deficiency in ascorbic acid can lead to symptoms such as dry skin, itchy skin, and bleeding. Supplementing with ascorbic acid can replenish the body's vitamin needs, improve symptoms such as dry and itchy skin, and promote the body's absorption of calcium, thus benefiting overall health.

[0206] Therefore, when NAD amino acid nutrient solution encapsulated in nanoliposomes is used in face masks, it has the following five major benefits:

[0207] Skin rejuvenation:

[0208] The Liposome + NAD + Amino Acid Mask uses highly efficient liposome technology to deeply penetrate the skin's bottom layer, repair damaged cells, restore the skin's softness and smoothness, and give you a baby-like skin feel.

[0209] Skin whitening:

[0210] Unique NAD+ formula combined with amino acid essence effectively inhibits melanin production, lightens spots, evens skin tone, and gives skin a healthy and natural white glow.

[0211] Tightening:

[0212] Liposome + NAD+ amino acid mask contains rich collagen and elastin, which can deeply nourish the skin, improve skin elasticity, tighten the skin contour, and delay the signs of skin relaxation.

[0213] Brightening:

[0214] NAD+ can activate energy metabolism in cells, promote skin metabolism, help the skin remove waste and toxins, and make the skin glow with a healthy and bright glow.

[0215] Water retention:

[0216] The strong moisturizing effect of amino acids combined with liposome technology forms a natural moisture barrier, locks in moisture for a long time, and keeps the skin smooth and moist for a long time.

[0217] Applicable population:

[0218] Liposome + NAD+ amino acid mask is not only suitable for young people, but also helps them lock in youth, and is more suitable for middle-aged and elderly people, helping them return to youth and radiate youthful vitality.

[0219] Liposome + NAD+ amino acid mask brings comprehensive care to the skin with five effects in one, providing comprehensive repair of skin, whitening, tightening, brightening, and water retention in one experience. The skin says goodbye to dullness and fatigue, and radiates a healthy and youthful glow.

Claims

1. A method for preparing a nanoliposome-encapsulated NAD amino acid nutrient solution, characterized by, Comprise: S1, preparation of raw materials: S1.1, NAD amino acid; S1.2, lecithin: use of pure natural lecithin from soybean, egg; S1.3, sodium hyaluronate: low molecular weight sodium hyaluronate is used; S1.4, amino acid complex: contains a variety of essential amino acids, including glycine, serine and lysine; S1.5, high purity nicotinamide; S1.6, ascorbic acid; S1.7, plant extract: combined with purslane extract, golden yellow chamomile extract; S2, preparation of nutrient solution: S2.1, preparation of liposome solution: mix lecithin with ethanol, heat to 50-55℃, stir until the phospholipid is completely dissolved, and get a uniform liposome solution; S2.2, mixing of active ingredients: mix lecithin, NAD amino acid, sodium hyaluronate, amino acid complex, nicotinamide, ascorbic acid, plant extract, according to the ratio of 10:5:3:2:1:0.5:1, heat to 30-35℃, stir until uniform, form active ingredient solution; S2.3, liposome wrapping: slowly drop the active ingredient solution into the liposome solution through the precise liquid device, start the ultrasonic disperser at the same time, the ultrasonic power is 100W, under the joint action of stirring and ultrasonic, the active ingredient is uniformly wrapped by the liposome; S2.4, removal of solvent: transfer the obtained mixed solution to the rotary evaporator, set the temperature to 40℃ and the vacuum degree to-0.08MPa, accurately control the evaporation speed of the solvent, and ensure the stability of the active ingredient and the liposome; S2.5, product modulation: add glycerol and potassium sorbate, and adjust the pH value to 5.5-6.

5.

2. The method for preparing a nano-liposome-encapsulated NAD amino acid nutrient solution as described in claim 1, characterized in that: In S1.1, the amino acid selects NAD+ precursor with purity higher than 98%, including NMN nicotinamide mononucleotide.

3. The method for preparing a nano-liposome-encapsulated NAD amino acid nutrient solution as described in claim 1, characterized in that: In S1.2, the lecithin selects HSPC hydrogenated lecithin.

4. The method for preparing a nano-liposome-encapsulated NAD amino acid nutrient solution as described in claim 1, characterized in that: In S1.6, ascorbic acid selects esterified vitamin C.

5. The method for preparing a nano-liposome-encapsulated NAD amino acid nutrient solution as described in claim 1, characterized in that: In S2.1, mix HSPC with ethanol according to the optimized ratio, heat to 52℃, and stir at a stirring speed of 300rpm until the phospholipid is completely dissolved.

6. The method for preparing a nano-liposome-encapsulated NAD amino acid nutrient solution as described in claim 1, characterized in that: In S2.2, first add HSPC, NMN, sodium hyaluronate and amino acid complex, then gradually add nicotinamide and ascorbic acid, and finally add plant extract, maintain 35℃ and 400rpm stirring speed throughout the process, form active ingredient solution; The whole process is mixed according to the ratio of 10:5:3:2:1:0.5:

1.

7. The method for preparing a nanoliposome-encapsulated NAD amino acid nutrient solution as described in claim 1, characterized in that: In S2.3, use high-frequency ultrasonic disperser, set the power to 120W, the frequency to 20kHz, and the processing time to 30 minutes, slowly drop the active ingredient solution into the liposome solution, and start the stirrer at the same time.

8. The method for preparing a nanoliposome-encapsulated NAD amino acid nutrient solution as described in claim 1, characterized in that: In S2.4, transfer the mixed solution to the rotary evaporator, set the temperature to 42℃ and the vacuum degree to-0.085MPa, until the solvent is completely evaporated.

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