Plant growth regulator containing 5-ALA and application of plant growth regulator in prunus humilis planting

Abstract

The invention belongs to the technical field of fruit tree cultivation and plant growth regulation, and particularly relates to a plant growth regulator containing 5-ALA and application of the plant growth regulator in prunus humilis planting. According to the method disclosed by the invention, the nutritional quality of fruits is remarkably improved by spraying a 100 mg / L 5-ALA solution on the whole cerasus humilis plant. Compared with clear water, the sugar content of the cerasus humilis fruits treated by the 5-ALA in the full ripening stage is increased by 44.01%, the acidity is reduced by 23.75%, the soluble solids are increased by 60.22%, and the flavor of the fruits is remarkably improved; meanwhile, the total calcium content of the fruits is increased by 30.22%, the water-soluble calcium content is increased by 91.12%, the pectin calcium content is reduced by 30.04%, and the active calcium content is increased by 50.74%; the content of soluble protein is increased by 30.98%, so that the acidity is reduced, and sugar, calcium and protein are synergistically improved.

Description

Technical Field

[0001] This invention belongs to the field of fruit tree cultivation and plant growth regulation technology, specifically relating to a plant growth regulator containing 5-ALA and its application in the cultivation of European plum. Background Technology

[0002] European plum (Cerasus humilis) is an important specialty fruit resource in northern my country. Its fruit is rich in vitamins, minerals, polyphenols, and amino acids, possessing high nutritional and health benefits. However, in actual production, due to differences in tree nutrient supply, environmental factors, and management conditions, European plum fruit tends to have high acidity and poor flavor. European plum is also known as the "calcium fruit" because of its high content of active calcium, which is easily absorbed and utilized by the human body. Soluble protein is an important indicator of nitrogen metabolism and nutritional quality in fruit; increasing its content is beneficial for improving the nutritional value and textural stability of the fruit. Currently, production often uses foliar calcium supplementation, plant growth regulators, or fertilizers to adjust the acid-sugar ratio, but these methods have limitations in terms of improvement, unstable effects, or significant effects on single indicators without sufficient overall quality improvement.

[0003] 5-ALA is a precursor for the synthesis of important metabolites such as chlorophyll and heme in plants, and it plays a role in promoting photosynthetic efficiency, enhancing mineral absorption, regulating carbon and nitrogen metabolism, and delaying fruit senescence. While there are reports of 5-ALA applications on some fruit trees, the specific timing, concentration, and frequency of application for reducing acidity and synergistically increasing total calcium, water-soluble calcium, pectin calcium, soluble protein, and sugar content in European plum fruit are not publicly available, and there is a lack of standardized technical solutions that can be directly promoted. Therefore, developing a safe, efficient, and easy-to-operate method for applying 5-ALA to significantly improve the acidity and nutritional quality of European plum fruit, including its sugar, calcium, and protein content, is of great significance for enhancing the marketability and industrial value of European plum. Summary of the Invention

[0004] To address the above problems, the present invention provides a plant growth regulator comprising 5-ALA.

[0005] This invention also provides the application of the above-mentioned plant growth regulator in the cultivation of European plum.

[0006] Furthermore, this includes the following steps:

[0007] The plant growth regulator described above should be sprayed on the whole plant before the coloring and swelling period of the plum fruit. Spray twice before the coloring and swelling period and once from the coloring and swelling period to the hard ripening period.

[0008] Furthermore, the final concentration of 5-ALA in the plant growth regulator is 100 mg / L.

[0009] Furthermore, the two sprays are applied 10 days apart before the coloring and swelling period.

[0010] Furthermore, spraying should be carried out between 8:00 AM and 10:00 AM.

[0011] The present invention has the following beneficial effects:

[0012] 1. The method of this invention significantly improves the nutritional quality of Prunus armeniaca fruit by spraying the entire plant with a 100 mg / L 5-ALA solution. Compared with the water control, the sugar content of Prunus armeniaca fruit at full ripeness increased by 44.01%, acidity decreased by 23.75%, and soluble solids increased by 60.22% after 5-ALA treatment, significantly improving fruit flavor. Simultaneously, the total calcium content increased by 30.22%, water-soluble calcium content increased by 91.12%, pectin calcium content decreased by 30.04%, and active calcium content increased by 50.74%; soluble protein content increased by 30.98%, achieving a synergistic improvement in acidity and sugar, calcium, and protein levels.

[0013] 2. The method of the present invention uses a specific concentration (100 mg / L) of 5-ALA to be sprayed three times before the coloring and swelling period and before the hardening period of European plum. This can effectively regulate the carbon and nitrogen metabolism and mineral absorption process in the later stage of fruit, significantly improve the calcium accumulation and protein synthesis level of fruit, and promote the conversion of organic acids into sugars and other substances, thereby reducing acidity and improving overall quality.

[0014] 3. The method of the present invention has a clear spraying time, appropriate number of spraying times, and simple operation. It can be directly embedded into the existing field management measures of European plum without adding extra cultivation burden. Moreover, 5-ALA is a natural precursor substance of plant metabolism, which is highly safe, friendly to fruit trees and the environment, and meets the requirements of green production.

[0015] 4. The method of this invention promotes calcium absorption and its effective utilization within the fruit by increasing total calcium, water-soluble calcium, and active calcium. The increase in water-soluble and active calcium enhances cell wall stability, improving the fruit's resistance to cracking, decay, and storage problems, thus extending its shelf life. Simultaneously, the reduced pectin calcium content decreases the amount of excessively cross-linked "bound calcium" in the cell walls, resulting in a finer, crisper texture and improved taste. Furthermore, it promotes the transfer of calcium from the cell walls to available sinks, leading to a more balanced and stable fruit texture.

[0016] 5. The method of this invention selects a sunny day between 8:00 and 10:00 AM for spraying, when the temperature and humidity are moderate, ensuring that the solution dries quickly and is fully absorbed by the fruit, thereby improving the spraying effect. In addition, the sunlight is relatively gentle, and strong winds are less likely, reducing solution evaporation, avoiding solution waste, and ensuring the accuracy and maximum effect of each spraying. Detailed Implementation

[0017] Various exemplary embodiments of the present invention are now described in detail. Unless otherwise specified, the methods used in the embodiments are conventional methods, and the reagents used are commercially available reagents or reagents prepared using conventional methods. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, characteristics, and embodiments of the present invention.

[0018] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0019] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0020] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be readily apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0021] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0022] Example 1

[0023] 1. Determining the spraying time. Based on the growth and development process of the European plum fruit, spraying should be carried out on a sunny morning between 8:00 and 10:00 before the fruit coloring and expansion stage and before the hardening stage. At this time, the temperature and humidity are moderate, which is conducive to the absorption of 5-ALA solution on the surface of leaves and fruit.

[0024] 2.5-ALA Solution Preparation. Prepare a 5-ALA solution with a final concentration of 100 mg / L. Taking 10 L of solution as an example, accurately weigh 1 g of 5-ALA powder, add a small amount of water, stir thoroughly to dissolve, and then bring the volume to 10 L. Prepare and use immediately.

[0025] 3.5-ALA solution for whole-plant spraying. Load the prepared 5-ALA solution into an electric sprayer and spray evenly over the entire Prunus cerasifera plant, ensuring small, even droplets form on the leaves and fruit surface without dripping. During spraying, adjust the nozzle angle to ensure even coverage of the fruit and leaves.

[0026] 4. Harvesting. Harvest the fruit after it has reached full ripeness. Select healthy fruit that is uniform in size, free from pests, diseases, and mechanical damage to ensure the representativeness and accuracy of the experimental results.

[0027] 5. Sample processing. The collected fruits were transported back to the laboratory in ice boxes, washed with distilled water and dried, then divided into 3 equal portions and flash-frozen in liquid nitrogen at -80 ℃ for storage until analysis.

[0028] 6. Fruit acidity determination. A handheld acidity meter was used for determination, and the results are shown in Table 1.

[0029] 7. Determination of soluble sugar content in fruit. 0.1 g of dried Prunus cerasifera fruit sample was weighed, and the sugar content was extracted with 80% ethanol. The sugar content was then determined by the anthrone-sulfuric acid method. The results are shown in Table 1.

[0030] 8. Determination of soluble solids in fruit. A handheld refractometer was used for the determination, and the results are shown in Table 1.

[0031] 8. Determination of total calcium content in fruit. 0.1 g of dried Prunus cerasifera fruit was weighed, and total calcium was extracted by sulfuric acid-hydrogen peroxide digestion method. The total calcium was determined by flame atomic absorption spectrophotometry. The results are shown in Table 2.

[0032] 9. Determination of water-soluble calcium and pectin calcium content in fruit. 0.5 g of fresh Prunus cerasifera fruit was weighed, and the contents of water-soluble calcium and pectin calcium were extracted using a stepwise extraction method with ultrapure water and 1 mol / L NaCl. The contents of water-soluble calcium and pectin calcium in the fruit were determined by flame atomic absorption spectrometry. Active calcium was the sum of water-soluble calcium and pectin calcium. The results are shown in Table 2.

[0033] 10. Determination of soluble protein content in fruit. 0.5 g of fresh Prunus cerasifera fruit was weighed, and the soluble protein content was extracted using phosphate buffer (pH 7.8). The soluble protein content was determined using the Coomassie brilliant blue method. The results are shown in Table 3.

[0034] Note: * indicates a significant difference between the treatment and control at the 0.05 level.

[0035] Note: * indicates a significant difference between the treatment and control at the 0.05 level.

[0036] Note: * indicates a significant difference between the treatment and control at the 0.05 level.

[0037] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A plant growth regulator, characterized by, The ingredient includes 5-ALA.

2. The use of the plant growth regulator of claim 1 in the cultivation of Cerasus humilis.

3. Use according to claim 2, characterized in that, The method comprises the following steps: Spraying the plant growth regulator on the whole plant before the fruit color expansion period, spraying twice before the color expansion period, and spraying once from the color expansion period to the hardening period.

4. Use according to claim 3, characterized in that, The final concentration of 5-ALA in the plant growth regulator is 100 mg / L.

5. Use according to claim 3, characterized in that, The interval between the two sprays before the color expansion period is 10 days.

6. Use according to claim 3, characterized in that, Spraying is carried out from 8:00 to 10:00 in the morning.

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