Anti-aging plant composition and use thereof

Abstract

The application belongs to the technical field of anti-aging and anti-oxidation, and particularly relates to an anti-aging plant composition and application thereof. The raw material of the anti-aging plant composition is Physocarpus and Paeonia; and the preparation method of the anti-aging plant composition comprises the following steps: firstly, mixing Physocarpus and Paeonia, and then water extraction to obtain an extraction solution; then, passing the extraction solution through a macroporous resin column, firstly eluting with 10-30% ethanol, discarding the eluent, then eluting with 50-70% ethanol, and collecting the eluent, thereby obtaining the anti-aging plant composition. The anti-aging plant composition prepared by the application can be used to prepare cosmetic products such as essence water or face cream, and can better improve the water content of skin, skin elasticity, skin wrinkles and scalp skin thickness, and has a good application prospect.

Description

Technical Field

[0001] This invention belongs to the field of anti-aging and antioxidant technology, specifically relating to an anti-aging plant composition and its application. Background Technology

[0002] Besides age, the most significant factors contributing to skin aging are free radical oxidation. Ultraviolet radiation, environmental pollution, and other environmental pollutants accelerate the production of free radicals in the body, thus accelerating skin aging, leading to loss of elasticity and dullness. Antioxidants can be used to eliminate free radicals, thereby delaying skin aging.

[0003] Current anti-aging cosmetics mainly rely on three core ingredients: retinols (such as retinol and its derivatives), hydroxypropyl tetrahydropyranotriol (HTP), and peptides (such as pentapeptides and hexapeptides). While these ingredients are effective in anti-aging, they still have certain limitations: retinols are easily oxidized and photosensitive, requiring strict sun protection after use; hydroxypropyl tetrahydropyranotriol, although gentle, has a slow onset of action; and peptides are expensive and have poor stability. In contrast, plant-derived anti-aging ingredients not only have multiple benefits (such as antioxidant, anti-inflammatory, and cell regeneration promotion), but are also generally gentler, safer, and suitable for long-term use.

[0004] In recent years, the application of plant-based compositions in anti-aging cosmetics has shown a significant upward trend. Studies have shown that combinations of multiple plant extracts, if properly formulated, yield more significant results. For example, the combination of Danish rock mint cell culture extract and yellow bell pepper extract exhibits both strong anti-aging activity and effective combating of harmful skin bacteria; while basil hairy root extract can delay skin aging and maintain moisture balance. This multi-target, multi-pathway mechanism of action makes plant-based compositions an important direction in the research and development of anti-aging cosmetics.

[0005] Capers are a medicinal and edible plant widely distributed in the Middle East and western my country. Their fruits, root bark, and leaves are rich in active ingredients, including volatile oils, alkaloids, and flavonoids. Modern pharmacological studies have shown that capers possess various pharmacological effects, including antibacterial, anti-inflammatory, antioxidant, antihypertensive, and antitumor properties, and have broad application prospects in the pharmaceutical, food, and cosmetic fields.

[0006] In terms of anti-aging, studies have shown that flavonoids in capers (such as naringin) have been proven to have significant antioxidant effects, which can scavenge free radicals, reduce oxidative stress damage, and regulate cellular metabolic pathways.

[0007] Peony (including white peony, red peony, etc.) and its extracts have significant effects on skin anti-aging and anti-oxidation, mainly through the following mechanisms:

[0008] Antioxidant effects: Peony is rich in paeoniflorin, polyphenols, flavonoids, and other active ingredients, which can effectively scavenge free radicals and reduce the damage of oxidative stress to the skin. Its antioxidant capacity is comparable to that of vitamin C, which can inhibit lipid peroxidation and delay collagen loss, thereby reducing wrinkle formation. Studies have shown that white peony extract can also enhance superoxide dismutase (SOD) activity, further strengthening the antioxidant effect.

[0009] Promotes cell regeneration: Paeoniflorin can improve the skin cell regeneration rate, enhance the activity of elastic fibers, and improve skin laxity and fine lines. At the same time, it indirectly delays aging by inhibiting the release of inflammatory factors (such as TNF-α and IL-6) and reducing skin damage caused by ultraviolet rays or environmental stimuli.

[0010] Moisturizing and Repairing: The polysaccharides and tannins in peony root can strengthen the skin barrier function, enhance moisturizing ability, and alleviate signs of aging caused by dryness. In addition, the flavonoids in peony can inhibit tyrosinase activity, reduce melanin deposition, and improve dull skin tone.

[0011] Despite the numerous advantages of plant-derived anti-aging ingredients, current technologies still have some significant shortcomings: most anti-aging cosmetics currently available, while having some effect, have a single mechanism of action and cannot comprehensively address the multiple factors of skin aging (such as skin elasticity, skin hydration, skin anti-wrinkle, scalp thinning, etc.), and their effectiveness in improving skin anti-aging needs further improvement.

[0012] Furthermore, the extraction and purification processes need optimization: Traditional plant extraction methods (such as reflux extraction and Soxhlet extraction) suffer from drawbacks such as low extraction efficiency, high energy consumption, and long processing times. In terms of purification, while high-speed countercurrent chromatography and polyamide column chromatography offer high purity, they require the use of various organic solvents (such as benzene, chloroform, and n-hexane), demanding stringent experimental conditions, causing significant pollution and high costs, making industrial-scale production difficult. When using chromatographic columns for purification, if conditions are unsuitable, the efficacy cannot be fully realized, and production costs are increased.

[0013] Therefore, it is of great significance to develop an anti-aging product that is safe, gentle, non-irritating, simple in formulation, low in cost, has no side effects, and has multiple and significant effects. Summary of the Invention

[0014] To address the shortcomings of existing technologies, this invention provides an anti-aging plant composition.

[0015] The technical solution adopted in this invention is as follows:

[0016] An anti-aging plant composition, wherein the raw materials of the anti-aging plant composition are capernaum and peony; the preparation method of the anti-aging plant composition is as follows:

[0017] (1) Mix capernaum and peony root, and extract with water to obtain an extract; the water extraction temperature is 80-95℃;

[0018] (2) Pass the extract through a macroporous resin column, first elute with 10-30% ethanol, discard the eluent, then elute with 50-70% ethanol, collect the eluent, and you have the product.

[0019] Capers are perennial vines and small semi-shrubs belonging to the genus Capers in the family Caperaceae. They are widely distributed in arid regions and have medicinal, edible, and ecological value. Their stems are highly branched, spreading horizontally or obliquely; leaves are nearly leathery, round or obovate; corollas are mostly white or pink, fruit flesh is red, and seeds are dark brown. Native to West Asia or Central Asia, in China they are mainly distributed in Xinjiang, Gansu, and Tibet. They are drought-tolerant and adaptable to poor soil conditions, including stony slopes and Gobi desert environments.

[0020] The roots, leaves, and fruits of capers can be used medicinally, possessing properties that dispel wind and dampness, promote blood circulation and relieve pain, and clear heat and detoxify. They are primarily used to treat rheumatoid arthritis, traumatic injuries, and sore throat. Modern research indicates that they contain alkaloids, flavonoids, and other components, exhibiting anti-inflammatory and antibacterial activities. Young shoots, leaves, and flower buds can be stir-fried or used as a condiment after being soaked in vinegar; in Europe, they are commonly used to flavor sauerkraut. Furthermore, capers help prevent wind erosion and maintain the stability of desert ecosystems, making them excellent sand-fixing plants and forage crops. The capers used in this invention are dried caper fruits.

[0021] Peony is a perennial herbaceous plant belonging to the genus Paeonia in the family Paeoniaceae. It has stout, dark brown roots, stems 40-70 cm tall, and bipinnate compound leaves. Flowers are solitary at the stem apex or leaf axils, blooming from May to June, and fruiting in August. Its flowers come in a rich variety of colors, including white, pink, red, and purple, with single or double petals and diverse flower shapes, earning it the title of "Queen of Flowers." Native to China, it is widely distributed in North China, East China, and Northwest China, and prefers sunny, well-drained sandy loam soil.

[0022] Medicinally, its roots are divided into white peony (for nourishing blood and regulating menstruation) and red peony (for clearing heat and cooling blood), rich in paeoniflorin, polyphenols, flavonoids, and other active ingredients, which can effectively scavenge free radicals and reduce oxidative stress damage to the skin. Its antioxidant capacity is comparable to vitamin C, inhibiting lipid peroxidation and delaying collagen loss, thereby reducing wrinkle formation. Studies have shown that white peony extract can also enhance superoxide dismutase (SOD) activity, further strengthening its antioxidant effect.

[0023] Preferably, the peony is white peony.

[0024] Preferably, the mass ratio of the capernaum to peony is 2:8-8:2.

[0025] Preferably, the water extraction is performed 1-3 times, and each water extraction takes 0.5-2 hours.

[0026] Preferably, the macroporous resin column packing is macroporous resin AB-8.

[0027] Preferably, in step (2), before eluting with 10-30% ethanol, the extract is loaded onto a macroporous resin column at a flow rate of 2-4 BV / h. After the resin adsorption is complete, the resin column bed is washed with water at a flow rate of 2-4 BV / h for 2-4 BV, and then elution is performed.

[0028] Preferably, the elution rate with 10-30% ethanol is 2-4 BV / h, and the elution volume is 2-4 BV.

[0029] Preferably, the elution with 50-70% ethanol is carried out at a flow rate of 2-4 BV / h and the elution volume is 2-4 BV.

[0030] Preferably, step (2) further includes concentrating the eluent into an extract, adding 50-70% (preferably 50-65%, most preferably 60%) of a polyol aqueous solution to the total mass of the capernaum and peony, stirring at 90-100°C for 30-60 minutes, and filtering until clear to obtain the anti-aging plant composition.

[0031] Preferably, the polyol is one or a combination of two or more of butanediol, propylene glycol, dipropylene glycol, glycerol, 1,3-propanediol, and ethanol.

[0032] Another objective of this invention is to provide an application of an anti-aging plant composition in the preparation of anti-aging essence water, cream, and face mask.

[0033] Beneficial effects of this invention:

[0034] (1) This invention achieves a significant enhancement of antioxidant and anti-aging effects through the synergistic combination of capers and peonies. Capers are rich in polyphenolic compounds, which have a strong ability to scavenge free radicals and can effectively inhibit oxidative stress damage to the skin; while the active ingredients such as paeoniflorin in peonies can promote collagen synthesis and enhance skin elasticity. After the two are combined, not only is the antioxidant activity improved compared to the single component, but it can also reduce the depth of wrinkles and improve skin roughness by regulating the skin microenvironment. Experiments show that after using the essence water containing this composition, the subjects' skin moisture content and elasticity index were significantly improved, and no irritation reaction was observed, confirming its safety and effectiveness.

[0035] (2) The preparation method of the present invention achieves efficient enrichment and purification of active ingredients through a specific preparation method. Under the gradient elution conditions of the present invention, the anti-aging components of capernaum and peony can be specifically retained. This process not only avoids the damage to active ingredients caused by high temperature extraction and concentration, but also improves the stability and transdermal absorption rate of the essence water or face cream. Its clinical test shows that after continuous use for 4 weeks, the area of ​​wrinkles on the skin of the subjects was reduced, and the composition has good compatibility in the formulation and is suitable for the development of various dosage forms.

[0036] (3) This invention further verifies the effectiveness of anti-aging essence water on scalp anti-aging. The results show that after 4 to 8 weeks of use, the scalp skin thickness of the subjects increased significantly, and the difference reached a highly statistically significant level compared with before use. Detailed Implementation

[0037] All raw materials used in this invention are commercially available conventional raw materials, and all percentage concentrations of ethanol are volume concentrations. The peony used below refers to white peony root.

[0038] Example 1

[0039] This embodiment provides an anti-aging essence water, the specific formula and preparation process of which are shown in Table 1 below:

[0040] Table 1

[0041]

[0042] The anti-aging plant composition is made from capers and peonies in a 1:1 mass ratio.

[0043] The preparation method of the anti-aging plant composition is as follows:

[0044] (1) Add 50g of capernaum and 50g of peony to 900g of water, heat to 90℃ and extract for 2h, filter until clear; concentrate the filtrate to 100g;

[0045] (2) 100g of filtrate was loaded onto macroporous resin (AB-8) at a flow rate of 3BV / h. After adsorption, the macroporous resin was rinsed with 3BV of deionized water at a flow rate of 3BV / h.

[0046] Take 3 BV of 20% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute with 3 BV and discard the eluent.

[0047] Take 3 BV of 60% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute 3 BV and collect the eluent.

[0048] The eluent was concentrated into an extract, and 60% propylene glycol aqueous solution was added to the extract to 100g (total mass of capernaum and peony 100g). The mixture was stirred at 95°C for 45min and filtered until clear to obtain an anti-aging plant composition.

[0049] The preparation method of anti-aging essence water is as follows:

[0050] 1) In the main beaker, xanthan gum, hyaluronic acid and Carbopol Ultrez 21 polymer were pre-dispersed in butanediol, then water was added and heated and stirred to dissolve. After being dissolved evenly, the mixture was cooled to 50°C to prepare phase A.

[0051] 2) After phase B is dissolved by gentle heating, add phase A, stir well, and cool to below 45℃;

[0052] 3) After the C phase is dissolved by stirring, add it to the system of step (2), stir evenly, and finally filter out the material.

[0053] Example 2

[0054] This embodiment provides an anti-aging essence water, which differs from Example 1 only in the dosage of medicinal materials and the preparation method of the anti-aging plant composition.

[0055] The preparation method of the anti-aging plant composition is as follows:

[0056] (1) Add 20g of capernaum and 80g of peony to 900g of water, heat to 90℃ and extract for 2h, filter until clear; concentrate the filtrate to 100g;

[0057] (2) 100g of filtrate was loaded onto macroporous resin (AB-8) at a flow rate of 3BV / h. After adsorption, the macroporous resin was rinsed with 3BV of deionized water at a flow rate of 3BV / h.

[0058] Take 2 BV of 10% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute for 3 BV and discard the eluent.

[0059] Take 4 BV of 50% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute for 3 BV and collect the eluent.

[0060] The eluent was concentrated into an extract, and 70% propylene glycol aqueous solution was added to the extract to a volume of 100g of the medicinal material. The mixture was stirred at 92°C for 55 minutes and filtered until clear to obtain an anti-aging plant composition.

[0061] Example 3

[0062] This embodiment provides an anti-aging essence water, which differs from Example 1 only in the dosage of medicinal materials and the preparation method of the anti-aging plant composition.

[0063] The preparation method of the anti-aging plant composition is as follows:

[0064] (1) Add 80g of capernaum and 20g of peony to 900g of water, heat to 90℃ and extract for 2h, filter until clear; concentrate the filtrate to 100g;

[0065] (2) 100g of filtrate was loaded onto macroporous resin (AB-8) at a flow rate of 3BV / h. After adsorption, the macroporous resin was rinsed with 3BV of deionized water at a flow rate of 3BV / h.

[0066] Take 3 BV of 30% ethanol and elute the macroporous resin at a flow rate of 2 BV / h. Elute for 4 BV and discard the eluent.

[0067] Take 3 BV of 65% ethanol and elute the macroporous resin at a flow rate of 4 BV / h. Elute for 4 BV and collect the eluent.

[0068] The eluent was concentrated into an extract, and 60% propylene glycol aqueous solution was added to the extract up to the amount of the medicinal material. The mixture was stirred at 98°C for 30 minutes and filtered until clear to obtain an anti-aging plant composition.

[0069] Example 4

[0070] This embodiment provides an anti-aging face cream, the formula and preparation process of which are shown in Table 2 below:

[0071] Table 2

[0072]

[0073] The preparation process of the anti-aging plant composition described in phase D is the same as in Example 1.

[0074] The preparation process of anti-aging face cream is as follows:

[0075] 1) After dispersing SEPINOV EMT-10 and sodium hyaluronate in butylene glycol in a main beaker, add the other raw materials in phase A, heat and stir until uniform, and the water bath temperature is 90℃.

[0076] 2) After the C phase is dissolved by gentle heating, set aside. In the B phase beaker, add all raw materials except DM 10, heat and stir until dissolved and emulsified. While heating and stirring in the main beaker, slowly pour the B phase into the main beaker, then add DM10, keep warm and stir for 3-5 minutes, then add the C phase. Then homogenize at about 6000 rpm for 3-5 minutes. Stir and cool to below 45℃.

[0077] 3) After the D phase is mixed evenly, add it to the main beaker and stir evenly to obtain the final product.

[0078] Example 5

[0079] This embodiment provides an anti-aging face cream, which differs from Example 4 only in the preparation method of the anti-aging plant composition, as detailed below:

[0080] (1) Add 50g of capernaum and 50g of peony to 600g of water, heat to 80℃ and extract for 1h, filter until clear; concentrate the filtrate to 100g;

[0081] (2) 100g of filtrate was loaded onto macroporous resin (AB-8) at a flow rate of 2BV / h. After adsorption was completed, the macroporous resin was rinsed with 2BV of deionized water at a flow rate of 2BV / h and washed for 2BV.

[0082] Take 2 BV of 10% ethanol and elute the macroporous resin at a flow rate of 2 BV / h. Elute for 3 BV and discard the eluent.

[0083] Take 2 BV of 50% ethanol and elute the macroporous resin at a flow rate of 2 BV / h. Elute for 3 BV and collect the eluent.

[0084] The eluent was concentrated into an extract, and 50% butanediol aqueous solution was added to the extract up to the amount of the medicinal material. The mixture was stirred at 95°C for 30 minutes and filtered until clear to obtain an anti-aging plant composition.

[0085] Example 6

[0086] This embodiment provides an anti-aging face cream, which differs from Example 4 only in the preparation method of the anti-aging plant composition, as detailed below:

[0087] (1) Add 50g of capernaum and 50g of peony to 1200g of water, heat to 95℃ and extract for 1h, filter until clear, collect the filtrate and keep the residue for later use; add 1200g of water to the residue, heat to 95℃ and extract for 1h, filter until clear, collect the filtrate and discard the residue; combine the filtrates and concentrate to 100g.

[0088] (2) 100g of filtrate was loaded onto macroporous resin (AB-8) at a flow rate of 4BV / h. After adsorption, the macroporous resin was rinsed with 4BV of deionized water at a flow rate of 4BV / h for 2BV.

[0089] Take 4 BV of 30% ethanol and elute the macroporous resin at a flow rate of 4 BV / h. Elute for 3 BV and discard the eluent.

[0090] Take 4 BV of 70% ethanol and elute the macroporous resin at a flow rate of 4 BV / h. Elute for 3 BV and collect the eluent.

[0091] The eluent was concentrated into an extract. 50% dipropylene glycol, 20% glycerol and 30% water were added to the extract to the amount of the medicinal material. The mixture was stirred at 95°C for 60 minutes and filtered until clear to obtain an anti-aging plant composition.

[0092] Comparative Example 1

[0093] This comparative example provides an anti-aging essence water, which differs from Example 1 in that the C phase is different, as detailed in Table 3 below:

[0094] Table 3

[0095]

[0096] The preparation method of the capernaum extract described in phase C is as follows:

[0097] (1) Add 900g of water to 100g of capers and heat to 90℃ for 2h to extract. Filter until clear. Concentrate the filtrate to 100g.

[0098] (2) 100g of filtrate was loaded onto macroporous resin (AB-8) at a flow rate of 3BV / h. After adsorption, the macroporous resin was rinsed with 3BV of deionized water at a flow rate of 3BV / h.

[0099] Take 3 BV of 20% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute with 3 BV and discard the eluent.

[0100] Take 3 BV of 60% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute 3 BV and collect the eluent.

[0101] The eluent was concentrated into an extract, and 60% propylene glycol aqueous solution was added to the extract up to the amount of the medicinal material. The mixture was stirred at 95°C for 45 minutes and filtered until clear to obtain the extract of Citrus aurantium.

[0102] Comparative Example 2

[0103] This comparative example provides an anti-aging essence water, which differs from Example 1 in that the C phase is different, as detailed in Table 4 below:

[0104] Table 4

[0105]

[0106] The preparation method of the peony extract described in phase C is as follows:

[0107] (1) Add 900g of water to 100g of peony root, heat to 90℃ and extract for 2h, filter until clear; concentrate the filtrate to 100g;

[0108] (2) 100g of filtrate was loaded onto macroporous resin (AB-8) at a flow rate of 3BV / h. After adsorption, the macroporous resin was rinsed with 3BV of deionized water at a flow rate of 3BV / h.

[0109] Take 3 BV of 20% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute with 3 BV and discard the eluent.

[0110] Take 3 BV of 60% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute 3 BV and collect the eluent.

[0111] The eluent was concentrated into an extract, and 60% propylene glycol aqueous solution was added to the extract up to the amount of medicinal material. The mixture was stirred at 95°C for 45 minutes and filtered until clear to obtain peony extract.

[0112] Comparative Example 3

[0113] This comparative example provides an anti-aging essence water, which differs from Example 1 only in the amount of medicinal materials used in the anti-aging plant composition, specifically: 10g of capernaum and 90g of peony.

[0114] Comparative Example 4

[0115] This comparative example provides an anti-aging essence water, which differs from Example 1 only in that it does not have phase C.

[0116] Comparative Example 5

[0117] This embodiment provides an anti-aging face cream, which differs from Example 4 only in the preparation method of the anti-aging plant composition, as detailed below:

[0118] (1) Add 450g of water to 50g of capers and heat to 90℃ for 2h to extract. Filter until clear. Concentrate the filtrate to 50g.

[0119] (2) 50g of filtrate was loaded onto macroporous resin (AB-8) at a flow rate of 3BV / h. After adsorption was completed, the macroporous resin was rinsed with 3BV of deionized water at a flow rate of 3BV / h.

[0120] Take 3 BV of 20% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute with 3 BV and discard the eluent.

[0121] Take 3 BV of 60% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute 3 BV and collect the eluent.

[0122] The eluent was concentrated to obtain capsula extract;

[0123] (3) Add 450g of water to 50g of peony root, heat to 90℃ and extract for 2h, filter until clear; concentrate the filtrate to 50g;

[0124] (4) 50g of filtrate was loaded onto macroporous resin (AB-8) at a flow rate of 3BV / h. After adsorption was completed, the macroporous resin was rinsed with 3BV of deionized water at a flow rate of 3BV / h.

[0125] Take 3 BV of 20% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute with 3 BV and discard the eluent.

[0126] Take 3 BV of 60% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute 3 BV and collect the eluent.

[0127] The eluent was concentrated to obtain peony extract;

[0128] (5) Combine the extracts of Citrus aurantium and Paeonia lactiflora, add 60% propylene glycol aqueous solution to the extracts to the amount of medicinal materials, stir at 95°C for 45 minutes, and filter until clear to obtain an anti-aging plant composition.

[0129] Comparative Example 6

[0130] This embodiment provides an anti-aging face cream, which differs from Example 4 only in the preparation method of the anti-aging plant composition, as detailed below:

[0131] (1) Add 50g of capernaum and 50g of peony root to 900g of water, heat to 70℃ and extract for 0.5h, then add 900g of water and heat under reflux for 1.5h, filter until clear, and combine the filtrates; concentrate the filtrate to 100g;

[0132] (2) 100g of filtrate was loaded onto macroporous resin (D101) at a flow rate of 3BV / h. After adsorption, the macroporous resin was rinsed with 3BV of deionized water at a flow rate of 3BV / h.

[0133] Take 3 BV of 20% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute with 3 BV and discard the eluent.

[0134] Take 3 BV of 60% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute 3 BV and collect the eluent.

[0135] The eluent was concentrated into an extract, and 60% propylene glycol aqueous solution was added to the extract up to the amount of the medicinal material. The mixture was stirred at 95°C for 45 minutes and filtered until clear to obtain an anti-aging plant composition.

[0136] Comparative Example 7

[0137] This embodiment provides an anti-aging face cream, which differs from Example 4 only in the preparation method of the anti-aging plant composition, as detailed below:

[0138] (1) Add 50g of capernaum and 50g of peony to 900g of water, heat to 90℃ and extract for 2h, filter until clear; concentrate the filtrate to 100g;

[0139] (2) 100g of filtrate was loaded onto macroporous resin (medium polarity macroporous adsorption resin 860021, Shandong Lukang Pharmaceutical Co., Ltd.) at a flow rate of 1BV / h. After adsorption, the macroporous resin was rinsed with 5BV of deionized water at a flow rate of 1BV / h for 3BV.

[0140] Take 5 BV of 10% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute for 3 BV and discard the eluent.

[0141] Take 5 BV of 80% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute for 3 BV and collect the eluent.

[0142] The eluent was concentrated into an extract, and 60% propylene glycol aqueous solution was added to the extract up to the amount of the medicinal material. The mixture was stirred at 95°C for 45 minutes and filtered until clear to obtain an anti-aging plant composition.

[0143] Comparative Example 8

[0144] This embodiment provides an anti-aging face cream, which differs from Embodiment 4 only in that it does not contain phase D.

[0145] Comparative Example 9

[0146] This comparative example provides an anti-aging essence water, which differs from Example 1 only in that peony is replaced with polygonatum, and capernaum is replaced with eleutherococcus senticosus and gentian root.

[0147] The preparation method is as follows:

[0148] (1) Add 25g of gentian root, 25g of Acanthopanax senticosus and 50g of Polygonatum sibiricum to 900g of water, heat to 90℃ and extract for 2h, filter until clear; concentrate the filtrate to 100g;

[0149] (2) 100g of filtrate was loaded onto macroporous resin (AB-8) at a flow rate of 3BV / h. After adsorption, the macroporous resin was rinsed with 3BV of deionized water at a flow rate of 3BV / h.

[0150] Take 3 BV of 20% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute with 3 BV and discard the eluent.

[0151] Take 3 BV of 60% ethanol and elute the macroporous resin at a flow rate of 3 BV / h. Elute 3 BV and collect the eluent.

[0152] The eluent was concentrated into an extract, and 60% propylene glycol aqueous solution was added to the extract up to the amount of the medicinal material. The mixture was stirred at 95°C for 45 minutes and filtered until clear to obtain an anti-aging plant composition.

[0153] Example 1: Efficacy Test of Anti-Aging Essence Water

[0154] 1. Experimental Methods

[0155] This experiment aims to recruit no fewer than 60 participants aged 30-60. Participants are required to refrain from changing their facial skincare products or using skincare products with similar effects during the testing period. They must also adhere to using the test sample twice daily, morning and evening, after cleansing their face. Only those who pass the screening and sign an informed consent form will be eligible for enrollment.

[0156] Day 0: After enrollment through screening, subjects were equilibrated in the testing environment for 30 minutes. A skin stratum corneum moisture content analyzer was used to test the moisture content of the stratum corneum, and a skin elasticity analyzer was used to test skin elasticity. A facial imaging system was used to image the subjects' faces. After cleansing their faces with a standardized facial cleanser, subjects used the test sample and equilibrated in the experimental environment for 30 minutes. The skin stratum corneum moisture content analyzer was used again to test the moisture content, and the skin elasticity analyzer was used to test skin elasticity. A facial imaging system was used to image the subjects' faces. Test samples were distributed to subjects, who were instructed to use the test sample after cleansing their faces morning and evening.

[0157] Day 14: Subjects were equilibrated in the testing environment for 30 minutes. The stratum corneum moisture content of the subjects' faces was tested using a skin stratum corneum moisture content tester, the skin elasticity of the subjects was tested using a skin elasticity tester, and the subjects' faces were imaged using a facial imaging system.

[0158] Day 28: Subjects were equilibrated in the testing environment for 30 minutes. The stratum corneum moisture content of the subjects' faces was tested using a skin stratum corneum moisture content tester, the skin elasticity of the subjects was tested using a skin elasticity tester, and the subjects' faces were imaged using a facial imaging system.

[0159] 2. Experimental Results

[0160] This experiment recruited 60 subjects with an average age of 42.6 ± 8.3 years. After screening, the subjects were enrolled and randomly divided into 6 groups using a random number table. Each group used a different test sample.

[0161] 2.1 Results of facial stratum corneum water content test

[0162] The MPA 580 multi-functional skin analyzer, manufactured by the German company CK, is equipped with a Corneometer CM 825 probe. This probe uses capacitance to detect the moisture content of the skin's surface layer. The principle is that the dielectric constant of water in the skin's surface layer is much higher than that of other components, so the change in the dielectric constant of the skin's surface layer is mainly determined by its moisture content. By measuring the dielectric constant of the skin's surface layer, the skin's moisture level can be calculated. The measurement results are presented as relative values ​​in CU (Corneometer Units), specifically used to measure skin moisture content. The higher the value, the more hydrated the skin's surface layer. See Table 5 for specific results.

[0163] Table 5

[0164]

[0165] Note: & p<0.05 vs before the test, && p<0.01 vs before the test, # p<0.05 vs Example 1.

[0166] Results description: Before the subjects used the product, there was no significant difference in the stratum corneum water content of the skin between Examples 1-3 and Comparative Examples 1-3; after the subjects used the product, the stratum corneum water content of the skin was significantly increased after 30 minutes of use of Examples 1-3 and Comparative Examples 1-3, and the stratum corneum water content of the skin was significantly increased after the second and fourth weeks of use of Examples 1-3.

[0167] 2.2 Skin elasticity R2 test results

[0168] The skin elasticity tester manufactured by CK in Germany is based on the principles of suction and stretching in physics. It measures the skin's mechanical response using precision sensors. Skin elasticity R² is the ratio of maximum rebound length to maximum stretch length. Skin elasticity R² is an evaluation index for the firming effect of cosmetics; the higher the value, the better the skin elasticity. If the skin elasticity R² after using the sample is significantly higher than before, it indicates that the product has a firming effect. Specific results are shown in Table 6.

[0169] Table 6

[0170]

[0171] Note: & p<0.05 vs before the test, && p<0.01 vs before the test.

[0172] Results description: Before the subjects used the product, there was no significant difference in skin elasticity R2 between Examples 1-3 and Comparative Examples 1-3; after the subjects used the product, the skin elasticity R2 of Examples 1-3 increased significantly after 30 minutes, the skin elasticity R2 of Examples 1 increased significantly after the second week, and the skin elasticity R2 of Examples 1-3 increased significantly after the fourth week.

[0173] 2.3 Results of wrinkle length test

[0174] The facial imaging system manufactured by Canfield, USA, captures high-resolution facial images from different angles using various light sources, including standard white light, ultraviolet light, and polarized light. These images are then analyzed and measured using a 3D skin analysis system manufactured by Miravex, Ireland. Wrinkle length is an evaluation indicator of the anti-wrinkle efficacy of cosmetics; a higher value indicates more severe wrinkles. If the wrinkle length after using the sample is significantly reduced compared to before use, it indicates that the sample has anti-wrinkle efficacy. Specific results are shown in Table 7.

[0175] Table 7

[0176]

[0177] Note: & p<0.05 vs before the test, && p<0.01 vs before the test, # p<0.05 vs Example 1.

[0178] Results description: Before the subjects used the product, there was no significant difference in wrinkle length between Examples 1-3 and Comparative Examples 1-3; after the subjects used the product, the difference in wrinkle length was significantly reduced after 30 minutes when using Examples 1-2, significantly reduced after the second week when using Examples 1-2, and significantly reduced after the fourth week when using Examples 1-3 and Comparative Example 3.

[0179] Example 2: Anti-aging serum cream efficacy test

[0180] 1. Experimental Methods

[0181] This experiment aims to recruit no fewer than 60 participants aged 30-60. Participants are required to refrain from changing their facial skincare products or using skincare products with similar effects during the testing period. They must also adhere to using the test sample twice daily, morning and evening, after cleansing their face. Only those who pass the screening and sign an informed consent form will be eligible for enrollment.

[0182] Day 0: After enrollment through screening, subjects were equilibrated in the testing environment for 30 minutes. A skin stratum corneum moisture content analyzer was used to test the moisture content of the stratum corneum, and a skin elasticity analyzer was used to test skin elasticity. A facial imaging system was used to image the subjects' faces. After cleansing their faces with a standardized facial cleanser, subjects used the test sample and equilibrated in the experimental environment for 30 minutes. The skin stratum corneum moisture content analyzer was used again to test the moisture content, and the skin elasticity analyzer was used to test skin elasticity. A facial imaging system was used to image the subjects' faces. Test samples were distributed to subjects, who were instructed to use the test sample after cleansing their faces morning and evening.

[0183] Day 14: Subjects were equilibrated in the testing environment for 30 minutes. The stratum corneum moisture content of the subjects' faces was tested using a skin stratum corneum moisture content tester, the skin elasticity of the subjects was tested using a skin elasticity tester, and the subjects' faces were imaged using a facial imaging system.

[0184] Day 28: Subjects were equilibrated in the testing environment for 30 minutes. The stratum corneum moisture content of the subjects' faces was tested using a skin stratum corneum moisture content tester, the skin elasticity of the subjects was tested using a skin elasticity tester, and the subjects' faces were imaged using a facial imaging system.

[0185] 2. Experimental Results

[0186] This experiment recruited 70 subjects with an average age of 44.8 ± 8.8 years. After screening, the subjects were enrolled and randomly divided into 7 groups using a random number table. Each group used a different test sample.

[0187] 2.1 Results of skin stratum corneum water content test

[0188] Table 8

[0189]

[0190] Note: & p<0.05 vs before the test, && p<0.01 vs before the test, # p<0.05 vs Example 1.

[0191] Results: Before product use, there was no significant difference in skin stratum corneum water content between Examples 4-6 and Comparative Examples 5-7 and 9. After product use, the skin stratum corneum water content significantly increased after 30 minutes, week 2, and week 4 with Examples 4-6 and Comparative Examples 5-7. The skin stratum corneum water content slightly increased after 30 minutes, week 2, and week 4 with Comparative Example 9, but the effect was slightly less pronounced than that of Examples 4-6 and Comparative Examples 5-7.

[0192] 2.2 Skin elasticity R2 test results

[0193] Table 9

[0194]

[0195] Note: & p<0.05 vs before the test, && p<0.01 vs before the test.

[0196] Results: Before product use, there was no significant difference in skin elasticity R2 between Examples 4-6 and Comparative Examples 5-7 and 9. After product use, skin elasticity R2 significantly increased after 30 minutes with Examples 4-6 and Comparative Examples 5-6, significantly increased after week 2 with Examples 5-6 and Comparative Examples 5-7, and significantly increased after week 4 with Examples 4-6 and Comparative Examples 5-7. Comparative Example 9 showed a slight increase in skin elasticity R2 after 30 minutes, week 2, and week 4, but the effect was slightly less pronounced than that of Examples 4-6 and Comparative Examples 5-7.

[0197] 2.3 Results of wrinkle length test

[0198] Table 10

[0199]

[0200] Note: & p<0.05 vs before the test, && p<0.01 vs before the test.

[0201] Results: Before product use, there was no significant difference in wrinkle length between Examples 4-6 and Comparative Examples 5-7 and 9. After product use, wrinkle length was significantly reduced after 30 minutes with Examples 4-6 and Comparative Examples 6-7 after weeks 2 and 4. Comparative Example 9 showed a slight reduction in wrinkle length after 30 minutes, weeks 2 and 4, but the effect was slightly worse than that of Examples 4-6 and Comparative Examples 5-7.

[0202] Example 3: Efficacy Test of Anti-aging Essence Water and Anti-aging Essence Cream

[0203] 1. Experimental Methods

[0204] The difference between the experimental methods and the results in Example 1 lies in the experimental grouping, as detailed below:

[0205] The plan is to recruit 60 people, divided into groups A and B, with 30 people in each group. Each group will use either the anti-aging essence water from Example 1 or Comparative Example 4. Each person will have their left and right sides of their face divided into two groups, using different anti-aging essence creams respectively. Everyone will first use the anti-aging essence water, then the anti-aging essence cream. Specific usage methods are shown in Table 11 below.

[0206] Table 11

[0207]

[0208] The experiment requires the recruitment of no fewer than 60 volunteers, aged 30-50, to test the skin's stratum corneum water content, skin elasticity R2, and wrinkle length.

[0209] Day 0: After enrollment through screening, subjects were equilibrated in the testing environment for 30 minutes. A skin stratum corneum moisture content analyzer was used to test the moisture content of the stratum corneum, and a skin elasticity analyzer was used to test skin elasticity. A facial imaging system was used to image the subjects' faces. After cleansing their faces with a standardized facial cleanser, subjects used the test sample and equilibrated in the experimental environment for 30 minutes. The skin stratum corneum moisture content analyzer was used again to test the moisture content, and the skin elasticity analyzer was used to test skin elasticity. A facial imaging system was used to image the subjects' faces. Test samples were distributed to subjects, who were instructed to use the test sample after cleansing their faces morning and evening.

[0210] Day 14: Subjects were equilibrated in the testing environment for 30 minutes. The stratum corneum moisture content of the subjects' faces was tested using a skin stratum corneum moisture content tester, the skin elasticity of the subjects was tested using a skin elasticity tester, and the subjects' faces were imaged using a facial imaging system.

[0211] Day 28: Subjects were equilibrated in the testing environment for 30 minutes. The stratum corneum moisture content of the subjects' faces was tested using a skin stratum corneum moisture content tester, the skin elasticity of the subjects was tested using a skin elasticity tester, and the subjects' faces were imaged using a facial imaging system.

[0212] 2. Experimental Results

[0213] The experiment recruited 60 volunteers with an average age of 49.1±12.4. The subjects were randomly divided into two groups, A and B, with 30 people in each group, and used different anti-aging essence waters. Each person's face was divided into two groups, left and right, and used different anti-aging essence creams.

[0214] 2.1 Results of skin stratum corneum water content test

[0215] Table 12

[0216]

[0217] Note: & p<0.05 vs before the test, && p<0.01 vs before the test, # p<0.05 vs B2, ## p<0.01 vs B2.

[0218] Results Description: Before product use, there was no significant difference in the stratum corneum water content among subjects A1, A2, B1, and B2. After product use, the stratum corneum water content significantly increased after 30 minutes of use of A1, A2, B1, and B2. The stratum corneum water content significantly increased after weeks 2 and 4 of use of A1, A2, and B1. Compared with B2, the difference in stratum corneum water content was significantly greater after week 2 of use of A1 and B1. The stratum corneum water content was significantly increased after week 4 of use of A1, A2, and B1.

[0219] 2.2 Skin elasticity R2 test results

[0220] Table 13

[0221]

[0222] Note: & p<0.05 vs before the test, && p<0.01 vs before the test, # p<0.05 vs B2, ## p<0.01 vs B2.

[0223] Results description: Before the subjects used the product, there was no significant difference in skin elasticity R2 among subjects A1, A2, B1, and B2. After the subjects used the product, the skin elasticity R2 of subjects A1, A2, and B1 was significantly improved after 30 minutes, week 2, and week 4. Compared with B2, the difference in skin elasticity R2 between subjects A1 and B1 was significantly improved after week 2, and the difference in skin elasticity R2 between subjects A1, A2, and B1 was significantly improved after week 4.

[0224] 2.3 Results of skin wrinkle length detection

[0225] Table 14

[0226]

[0227] Note: & p<0.05 vs before the test, && p<0.01 vs before the test, # p<0.05 vs B2, ## p<0.01 vs B2.

[0228] Results description: Before the subjects used the product, there was no significant difference in wrinkle length among subjects A1, A2, B1, and B2. After the subjects used the product, the wrinkle length was significantly reduced after 30 minutes, week 2, and week 4 when using A1, A2, and B1. Compared with B2, the difference in wrinkle length was significantly reduced after week 2 when using A1 and B1, and significantly reduced after week 4 when using A1, A2, and B1.

[0229] Example 4: Scalp anti-aging test of anti-aging essence water.

[0230] 1. Experimental Methods

[0231] This experiment aims to recruit no fewer than 30 participants aged 30-60. Participants are required to refrain from changing their scalp care products or using scalp care products with similar effects during the testing period, and to consistently use the test sample (anti-aging essence water) after each shampoo. Only those who pass the screening and sign the informed consent form will be eligible for enrollment.

[0232] Day 0: After being selected and enrolled, participants were equilibrated in the testing environment for 30 minutes. A skin ultrasound testing device was used to measure the scalp thickness in the Baihui acupoint area on the top of the head. Test samples were distributed to participants, who were instructed to use the test sample (anti-aging essence) after each shampoo.

[0233] Four weeks later: Subjects were kept in the testing environment for 30 minutes, and the thickness of the scalp in the Baihui acupoint area on the top of the subject was measured using a skin ultrasound tester.

[0234] Eight weeks later: Subjects were balanced in the test environment for 30 minutes, and the thickness of the scalp in the Baihui acupoint area on the top of the subject's head was measured using a skin ultrasound tester.

[0235] 2. Experimental Results

[0236] This experiment recruited 30 subjects with an average age of 43.7 ± 8.6 years. After screening, the subjects were enrolled and randomly divided into 3 groups using a random number table, with each group using a different test sample.

[0237] 2.1 Skin thickness measurement results

[0238] Table 15

[0239]

[0240] Note: & p<0.05 vs before the test, && p<0.01 vs before the test.

[0241] Results description: Before the subjects used the product, there was no significant difference in scalp skin thickness among the groups; after 4 weeks and 8 weeks of product use, the scalp skin thickness increased significantly.

[0242] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of protection of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the appended claims.

Claims

1. An anti-aging plant composition, characterized in that, The raw materials for the anti-aging plant composition are capernaum and peony; the preparation method of the anti-aging plant composition is as follows: (1) Mix capernaum and peony, and extract with water to obtain an extract; (2) Pass the extract through a macroporous resin column, first elute with 10-30% ethanol, discard the eluent, then elute with 50-70% ethanol, collect the eluent, and the extract is obtained; The mass ratio of the capernaum and peony is 2:8 to 8:2; The macroporous resin column packing material is of type AB-8 macroporous resin; Step (2) also includes concentrating the eluent into an extract, adding 50-70% of a polyol aqueous solution to the total mass of the caperca nigra and peony, stirring at 90-100℃ for 30-60 minutes, and filtering until clear to obtain the anti-aging plant composition. The polyol is one or a combination of two or more of butanediol, propylene glycol, dipropylene glycol, and glycerol.

2. The anti-aging plant composition according to claim 1, characterized in that, The water extraction temperature is 80~95℃, the number of extractions is 1-3 times, and the extraction time is 0.5-2h.

3. The anti-aging plant composition according to claim 1, characterized in that, Before eluting with 10-30% ethanol in step (2), the extract is loaded onto a macroporous resin column at a flow rate of 2-4 BV / h. After the resin adsorption is complete, the resin column bed is washed with water at a flow rate of 2-4 BV / h for 2-4 BV before elution.

4. The anti-aging plant composition according to claim 1, characterized in that, The elution rate with 10-30% ethanol is 2-4 BV / h, and the elution volume is 2-4 BV.

5. The anti-aging plant composition according to claim 1, characterized in that, The elution rate with 50-70% ethanol is 2-4 BV / h, and the elution volume is 2-4 BV.

6. The use of the anti-aging plant composition according to any one of claims 1-5 in the preparation of anti-aging essence water, cream, and face mask.

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