Oxidative stress related hair removal method based on aggregation-induced emission (AIE) materials

Abstract

The invention provides an oxidative stress related hair removal method based on an aggregation-induced emission (AIE) material, and relates to the technical field of beauty care. The invention relates to an oxidative stress related hair removal method based on an aggregation-induced emission (AIE) material. The method comprises the following specific operation steps: S1, pre-treating skin in an area needing hair removal; s2, TCSVP-C4 is smeared on the skin of the treatment area, and after standing, the TCSVP-C4 is erased and aired; s3, carrying out light activation treatment on the skin surface; s4, cleaning, nursing and strengthening the treatment course after the operation. After three times of treatment, the hair regeneration speed is obviously slowed down, and no obvious regeneration occurs within 21 days; the hair regeneration density is reduced by 20%-30% compared with that of a laser hair removal group, the hair is thinner and softer, the effect can be maintained for more than 21 days, and the problem that the hair removal effect is not lasting in the prior art is solved.

Description

Technical Field

[0001] This invention relates to the field of cosmetic care technology, specifically to an oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials. Background Technology

[0002] With socio-economic development and the upgrading of aesthetic demands, hair management has evolved from a traditional functional need into a technical field that also has aesthetic value. Current mainstream hair removal technologies are mainly divided into two categories: mechanical hair removal and thermodynamic hair removal, but both have significant technical bottlenecks.

[0003] Mechanical hair removal methods (such as razors and depilatory creams) remove hair shafts from the epidermis through physical action. While they offer immediate results, they cannot destroy hair follicle stem cells and require frequent treatment (1-2 times per week). Clinical data shows that frequent shaving can increase hair cross-section thickness by up to 27% and easily lead to folliculitis (incidence rate 18.7%) and epidermal damage (abrasion incidence rate 32%).

[0004] Thermodynamic hair removal methods, represented by laser / intense pulsed light (IPL), rely on the selective photothermal action of melanin in hair follicles to destroy their activity. However, this technology has strict limitations regarding hair color and skin type: its effectiveness on type III and below (light-colored) hair is less than 40%, and the risk of epidermal burns is 3.8 times higher in people with darker skin tones (Fitzpatrick type IV-VI). Furthermore, traditional laser hair removal devices have low heat dissipation efficiency (heat accumulation temperature >50℃), requiring complex airflow designs, resulting in bulky and expensive equipment.

[0005] From the current state of hair removal technology, mainstream solutions have significant technical shortcomings: Physical hair removal (such as shaving) only works on the surface of the skin and cannot reach the hair follicle, resulting in a short hair regrowth cycle (1-3 days). Repeated mechanical stimulation can easily lead to hair follicle hyperplasia and thicker hair, and is also accompanied by the risk of skin scratches and infection. Chemical hair removal (such as depilatory cream) relies on strong corrosive ingredients such as potassium thioglycolate to dissolve the hair structure. Although it can remove slightly deeper hair, the effect only lasts for 1-2 weeks, and the chemical components have a skin barrier damage rate of over 35%, with an allergic redness and swelling incidence rate of over 20%. Energy hair removal (such as laser hair removal) can act on the hair follicle through selective photothermal effects, but it has the drawbacks of "three highs and one low"—high equipment cost (over 100,000 yuan per unit), high treatment cost (hundreds to thousands of yuan per session), high operation threshold (requires a professional physician), and poor effect on light-colored hair (low melanin content). The risk of pigmentation and scarring after treatment is as high as 15%. None of the above technologies have solved the problem of balancing "long-lasting effect" and "safety", nor have they addressed the needs of skin health maintenance or the hair removal needs of people with light skin. Summary of the Invention

[0006] (a) Technical problems to be solved To address the shortcomings of existing technologies, this invention provides an oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials, which solves the problems of existing hair removal technologies such as short-lasting effects, low safety, numerous side effects, and limited applicability.

[0007] (II) Technical Solution To achieve the above objectives, the present invention provides the following technical solution: an oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials, comprising the following specific operational steps: S1. Pre-treat the skin in the area requiring hair removal; S2. Apply TCSVP-C4 to the treated area of ​​skin, leave it on, then wipe it off and let it air dry; S3. Photoactivation treatment of the skin surface; S4. Postoperative cleaning, care and intensive treatment.

[0008] TCSVP-C4 is a high-performance aggregation-induced emission material. (i) It breaks through the limitations of traditional photosensitizers and overcomes the aggregation-induced quenching (ACQ) effect by leveraging its aggregation-induced emission properties, thus avoiding the problem of low efficiency in generating reactive oxygen species due to aggregation. (ii) Relying on a unique intramolecular motion-restricted (RIM) mechanism, it plays a more significant role in restricted environments such as aggregation states, and can precisely adapt to the local microenvironment of hair follicles. (iii) Its optical and biological safety is highly compatible with the needs of medical aesthetics. It only works when activated by light and has no cytotoxicity when there is no light or under ordinary indoor lighting conditions. The timing and scope of action can be precisely controlled, effectively reducing the risk of damage to non-target tissues. (iv) Through alkyl chain engineering strategies, its lipophilicity and hydrophilic cationic groups in the molecular structure can be precisely adjusted, enhancing the affinity of TCSVP-C4 to the surface of negatively charged hair follicle cell membranes. This helps the material target hair follicles more accurately and efficiently, improving the hair removal effect and comprehensively meeting the core requirements of "precision, efficiency, and safety" for medical aesthetic hair removal.

[0009] Preferably, the specific process for pre-treating the skin in the area to be hair removal in step 1 is as follows: thoroughly clean the skin in the treatment area with water or a non-irritating cleanser to remove oil and dirt from the skin surface; for areas with longer hair, shave the hair on the surface of the treatment area with a disposable safety razor, leaving 0.5-1mm stubble to facilitate TCSVP-C4 contact with the hair follicle opening.

[0010] Preferably, the specific procedure for applying TCSVP-C4 to the skin of the treatment area in step 2, leaving it to stand, wiping it off, and letting it air dry is as follows: 1) Use a sterile applicator to evenly apply TCSVP-C4 to the treatment area, with a thickness of 0.1-0.2 mm, and the coverage area extends 1-2 cm beyond the target area. The concentration of TCSVP-C4 is 10 mmol / L, and the solvent is DMSO. 2) Let it sit in a dark place for 5 minutes to allow the material to fully penetrate into the hair follicle opening and the superficial dermis. Avoid strong light exposure during this period. 3) Soak a cotton cloth or gauze in 70% alcohol and gently wipe away the surface material. Then wait 10-20 minutes in a dark place to let the skin dry.

[0011] Preferably, the specific process for photoactivation treatment of the skin surface in step 3 is as follows: 1) Apply medical cooling gel to the skin surface to lower skin temperature; 2) Activate the customized light source equipment, adjust the light spot coverage of the treatment area, and set the parameters as follows: light intensity 45mW / cm². 2 Irradiate for 5-10 minutes, depending on the patient's skin sensitivity; monitor skin temperature in real time during irradiation, and control the skin surface temperature to ≤40℃ to avoid burns. If the temperature exceeds the threshold, irradiation should be stopped.

[0012] Preferably, the specific procedures for postoperative cleaning, nursing, and intensive treatment in step 4 are as follows: 1) Postoperative cleaning: Use a non-irritating cleanser to thoroughly clean the treated area to prevent material residue from damaging the skin if left for too long; 2) Postoperative care: Apply aloe vera gel and moisturizer to the treated area to moisturize the skin.

[0013] 3) Treatment course: Repeat the treatment on the 4th and 10th day after the first treatment, for a total of 3 treatments, to form a continuous regulation of hair follicles and achieve long-term hair removal effect.

[0014] Preferably, the light source is a Fenix ​​E28R V2.0 flashlight, operating at low brightness with a brightness of 60 lumens and an actual luminous intensity of 45 mW / cm². 2 The irradiation distance is fixed at 25cm.

[0015] The flashlight can work continuously for 17 hours and 15 minutes on low brightness, and a single device can support more than 100 continuous treatments (each treatment lasts 8-10 minutes), meeting the needs of long-term, multi-treatment use. It has an IP68 waterproof and dustproof rating, and the shell is made of non-slip, wear-resistant metal, ensuring stability and reliability in various usage scenarios (such as bathroom hair removal, wet scalp environments, etc.), and high safety for handheld operation. The fixed distance is 25cm (which can be controlled with a ruler or markers). At this distance, the light forms a uniform spot with a diameter of approximately 5-8cm, suitable for common treatment areas such as the scalp, limbs, and armpits. When the flashlight is on low brightness, the surface temperature is ≤35℃. Combined with the 25cm irradiation distance, the skin temperature range is 37-40℃ (safe range), eliminating the risk of burns.

[0016] Working principle: (1) AIE materials are a class of substances that hardly emit light in the single-molecule state, but emit strong fluorescence in the aggregated state or solid state. Its luminescence mechanism is mainly the intramolecular motion restriction mechanism. Compounds with propeller-shaped or shell-shaped structures can freely rotate or vibrate to dissipate energy after being excited by light in dilute solutions, and the molecules do not emit fluorescence; when the intramolecular motion is restricted due to aggregation or other reasons, the intramolecular motion is blocked and strong fluorescence is emitted. TCSVP-C4 has a typical D-π-A structure, combined with a twisted geometric configuration and the relative separation characteristics of the highest occupied molecular orbital and the lowest unoccupied molecular orbital, which endows it with a strong ability to generate reactive oxygen species in the aggregated state, and can act on hair follicle cells efficiently; the hydrophilic cationic groups and alkyl chain design in the TCSVP-C4 molecular structure can enhance the affinity with the negatively charged hair follicle cell membrane surface, helping the material to target hair follicles more accurately and efficiently.

[0017] (2) PDT generates ROS under light irradiation through photosensitizers (TCSVP-C4, which functions under light activation), thereby controllably inducing cellular oxidative stress. ROS interferes with the cellular state of the target area of ​​the hair follicle by affecting the cell membrane and subcellular structure.

[0018] (3) Current research shows that PDT mainly damages the hair follicle matrix and sebaceous glands, and does not directly damage hair follicle bulge stem cells (the key to maintaining hair follicle regeneration) and dermal papilla (the key to regulating the hair follicle cycle). However, after multiple PDTs, the toxic byproducts released by the necrosis of hair follicle matrix and sebaceous glands can cumulatively damage the adjacent bulge stem cells and dermal papilla through the "bystander effect".

[0019] (4) The Wnt / β-catenin pathway (also known as the “classical Wnt pathway”) is a core signaling pathway that regulates hair follicle development, cyclical growth and stem cell maintenance. Its activity changes directly determine the formation, proliferation, differentiation and resting state transition of hair follicles. Its specific role can be explored from the two stages of hair follicle development: the “embryonic period” and the “adult cyclical growth”. It also involves the fate regulation of hair follicle stem cells (HFSC).

[0020] 1) Embryonic period: In embryonic skin epidermal cells, the Wnt / β-catenin pathway is activated first, prompting epidermal cells to express hair follicle-specific markers (such as LEF1 and TCF3) and protrude towards the dermis to form hair follicle primordia—the first key structure in hair follicle formation. If the pathway activity is insufficient, the primordia cannot form, resulting in follicle-free skin. Activated epidermal primordia secrete signaling molecules (such as Wnt10b), further inducing the aggregation of underlying dermal cells to form dermal coagulation (the dermal precursor of the hair follicle); at the same time, dermal coagulation feedback signals (such as Shh) enhance the activity of Wnt / β-catenin in epidermal primordia, forming an "epidermal-dermal interaction loop," promoting the differentiation of primordia into mature hair follicle structures. As the pathway continues to be activated, hair follicle primordia gradually differentiate into different functional layers. The outer layer consists of hair follicle epithelial stem cells (located in the bulge zone); the inner layer consists of hair matrix cells (which proliferate actively and produce hair fibers) and dermal papilla cells (signaling centers that maintain hair follicle activity). If the pathway is abnormally activated during the differentiation stage, it will lead to disordered hair follicle stratification (such as abnormal hair structure). If the activity is lost, differentiation will stop and mature hair cannot be formed.

[0021] 2) The "Cyclical Growth" of Adult Hair Follicles: Adult hair follicles do not grow continuously, but follow a cyclical pattern of "Anagen phase → Catagen phase → Telogen phase" (the human hair follicle cycle is approximately 2-7 years, and in mice, approximately 3 weeks). The Wnt / β-catenin pathway is the core switch regulating this cycle transition. During the anagen phase, the Wnt / β-catenin pathway exhibits high activity, activating hair follicle stem cells (HFSCs) in the bulge region of the hair follicle, causing them to transition from a quiescent state to a proliferative state and migrate to the hair matrix; maintaining the continuous proliferative capacity of hair matrix cells, promoting hair fiber (keratin) synthesis; and regulating the secretion of signals by dermal papilla cells (such as FGF and IGF), further enhancing hair follicle growth signals. During the catagen phase, its activity rapidly declines, and the closure of pathway activity leads to the cessation of hair matrix cell proliferation and the initiation of apoptosis; the hair follicle structure gradually atrophies (e.g., hair root shortening), and the connection between the hair and the hair follicle loosens, preparing for the "telogen phase." During the resting phase, the pathway maintains low activity (basal level), keeping HFSCs in a quiescent state and preventing excessive consumption. When the pathway is reactivated by external signals (such as hormones or damage repair signals), the resting phase ends and the hair follicle enters the next growth phase.

[0022] TCSVP-C4 inhibits hair follicle activity by suppressing the Wnt / β-catenin pathway.

[0023] (5) The NF-κB (nuclear factor κB) signaling pathway has important applications in hair removal technology, mainly by regulating the growth, differentiation and apoptosis of hair follicle cells to achieve the hair removal effect.

[0024] 1) Inducing hair follicle cell apoptosis: After the NF-κB signaling pathway is activated, it promotes the expression of apoptosis-related proteins (such as FasL and Caspase-3) in hair matrix cells (the core cells responsible for hair proliferation), inducing a large number of hair matrix cells to apoptosis; at the same time, it inhibits the Wnt / β-catenin pathway, cuts off the proliferation signal of hair matrix cells, and finally causes hair follicles to enter the "atrophy and degeneration" stage from "active growth".

[0025] 2) Inhibit the proliferation and differentiation of hair follicle stem cells: The core of the resting phase of hair follicles is that hair follicle stem cells (HFSCs) are in a quiescent state. The NF-κB pathway prevents HFSCs from entering the proliferative state prematurely by inhibiting the expression of stem cell activation-related genes (such as Lgr5 and Myc) in HFSCs; and regulates the secretion of signals (such as TGF-β) by dermal papillary cells (DPCs), further enhancing the quiescent characteristics of HFSCs.

[0026] Inflammatory factors such as IL-1β and TNF-α can promote inflammatory responses and induce hair follicle degeneration by activating the NF-κB signaling pathway.

[0027] (III) Beneficial Effects This invention provides an oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials. It has the following beneficial effects: 1. Significant and long-lasting hair removal effect: After 3 treatments, the hair regrowth rate slows down significantly, with no obvious regrowth within 21 days; the hair regrowth density is reduced by 20%-30% compared to the laser hair removal group, and the hair is finer and softer. The effect can last for more than 21 days, solving the problem of the short-lasting effect of existing hair removal technologies.

[0028] 2. High safety, few side effects, and synergistic skin rejuvenation effect: After treatment, only mild and temporary skin redness occurs (subsiding within 1-2 hours), and no pigmentation, scarring, or damage to the skin barrier function is observed; at the same time, the skin becomes smoother after treatment. This initially achieves a synergistic effect of "hair removal + skin rejuvenation," overcoming the shortcomings of existing technologies such as low safety and numerous side effects.

[0029] 3. Relatively simple operation: Compared to the complex process and professional requirements of laser hair removal, this technology shows significant advantages in terms of ease of operation and treatment efficiency. Its usage is similar to that of hair removal cream, requiring no complex instrument operation skills, making it extremely easy to operate and greatly reducing the barrier to entry for home use or non-professional settings.

[0030] 4. Wider range of applications: In terms of the applicable population coverage of hair removal technology, traditional laser hair removal has long been limited by the dual constraints of skin color and hair color. However, this technology does not require the use of melanin in the hair follicle or skin as an energy absorption medium. From the mechanism of action, it avoids the constraints caused by skin color and hair color, breaks through this key bottleneck, and achieves wide adaptability to people with different skin colors and hair colors. This greatly expands the application boundaries of hair removal technology and further enhances the practicality and market value of the technology.

[0031] 5. Low cost and easy to promote: TCSVP-C4 has a low purchase price (50 yuan / mg), and the light therapy equipment is easy to operate (no professional physician qualifications are required, and it can be operated after training), making it easy for primary medical institutions and beauty institutions to promote and apply. The total cost for a single use is 70 yuan. Attached Figure Description

[0032] Figure 1 This image shows a comparison of the initial effects of the oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials proposed in this invention and conventional hair removal methods. Figure 2 This is a comparison chart of the effects of the oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials proposed in this invention and conventional hair removal methods on the third day. Figure 3This is a comparison chart of the effects of the oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials proposed in this invention and conventional hair removal methods on the seventh day. Figure 4 This is a comparison chart of the effects of the oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials proposed in this invention and conventional hair removal methods on the fourteenth day. Figure 5 This is a comparison chart showing the effects of the oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials proposed in this invention and conventional hair removal methods on the 21st day. Detailed Implementation

[0033] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art based on photodynamic hair removal technology without creative effort are within the scope of protection of the present invention.

[0034] Example 1: This invention provides an oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials, comprising the following specific steps: S1. Pre-treat the skin in the area requiring hair removal; S2. Apply TCSVP-C4 to the treated area of ​​skin, leave it on, then wipe it off and let it air dry; S3. Photoactivation treatment of the skin surface; S4. Postoperative cleaning, care and intensive treatment.

[0035] TCSVP-C4 type AIE material is selected as the core functional carrier for hair removal. Through precise matching of molecular structure and performance, it overcomes the technical shortcomings of traditional photosensitizers. Utilizing the restricted intramolecular motion (RIM) mechanism, the luminescence intensity is significantly enhanced in the localized aggregation state of the hair follicle, avoiding the problem of low efficiency in generating reactive oxygen species (ROS) due to aggregation, ensuring the induction of oxidative stress in hair follicle cells. Possessing a typical D-π-A structure and hydrophilic cationic groups, it can enhance the affinity with negatively charged hair follicle cell membranes, achieving precise targeting of hair follicles. Simultaneously, its twisted geometric configuration and orbital separation characteristics endow it with a strong ROS generation capacity, effectively intervening in the state of hair follicle cells and inhibiting hair growth. The optimal concentration for use is 10 mmol / L (solvent: DMSO). At this concentration, the material is non-cytotoxic without light exposure, only exerting its effect after photoactivation, avoiding damage to non-target tissues. Furthermore, normal lighting sources cannot effectively activate it, making its biocompatibility far superior to traditional chemical hair removal agents (such as potassium thioglycolate, with an allergy rate exceeding 20%).

[0036] The specific procedure for pre-treating the skin in the area to be treated in step 1 is as follows: thoroughly clean the skin in the treatment area with water or a non-irritating cleanser to remove oil and dirt from the skin surface; for areas with longer hair, use a disposable safety razor to shave the hair on the surface of the treatment area, leaving 0.5-1mm stubble to facilitate TCSVP-C4 contact with the hair follicle opening.

[0037] The specific procedure for applying TCSVP-C4 to the treated area of ​​skin in step 2, leaving it on, wiping it off, and letting it air dry is as follows: 1) Use a sterile applicator to evenly apply TCSVP-C4 to the treatment area, with a thickness of 0.1-0.2 mm, and extend the coverage area 1-2 cm beyond the target area. The concentration of TCSVP-C4 is 10 mmol / L, and the solvent is DMSO. 2) Let it sit in a dark place for 5 minutes to allow the material to fully penetrate into the hair follicle opening and the superficial dermis. Avoid strong light exposure during this period. 3) Soak a cotton cloth or gauze in 70% alcohol and gently wipe away the surface material. Then wait 10-20 minutes in a dark place to let the skin dry.

[0038] 10 mmol / L is the experimentally validated optimal concentration, balancing ROS generation efficiency and biosafety.

[0039] The sterile application brush head is made of medical-grade silicone (soft and non-irritating, avoiding skin abrasions), and the handle is made of medical-grade ABS plastic. The bristle length is 5mm, and the brush head width is 1-2cm (suitable for different hair removal areas). This ensures that a single application forms a uniform AIE material layer of 0.1-0.2mm thickness, avoiding waste due to excessively thick material or insufficient coverage of hair follicles due to excessively thin material. It is only for applying TCSVP-C4 material (concentration 10mmol / L, solvent DMSO), ensuring precise coverage of the target area and avoiding cross-contamination.

[0040] The specific procedure for photoactivation treatment of the skin surface in step 3 is as follows: 1) Apply medical cooling gel to the skin surface to lower skin temperature; 2) Activate the customized light source equipment, adjust the light spot coverage of the treatment area, and set the parameters as follows: light intensity 45mW / cm². 2 Irradiate for 5-10 minutes, depending on the patient's skin sensitivity; monitor skin temperature in real time during irradiation, and control the skin surface temperature to ≤40℃ to avoid burns. If the temperature exceeds the threshold, irradiation should be stopped.

[0041] The specific procedures for postoperative cleaning, nursing care, and intensive treatment in step 4 are as follows: 1) Postoperative cleaning: Use a non-irritating cleanser to thoroughly clean the treated area to prevent material residue from damaging the skin if left for too long; 2) Postoperative care: Apply aloe vera gel and moisturizer to the treated area to moisturize the skin.

[0042] 3) Treatment course: Repeat the treatment on the 4th and 10th day after the first treatment, for a total of 3 treatments, to form a continuous regulation of hair follicles and achieve long-term hair removal effect.

[0043] The light source is a Fenix ​​E28R V2.0 flashlight, operating at low brightness with a brightness of 60 lumens and an actual luminous intensity of 45 mW / cm². 2 The irradiation distance is fixed at 25cm.

[0044] The process of this invention is compatible with home use, small beauty salons and other scenarios. The only auxiliary tools required are a sterile application brush and a ruler (to control the distance of 25cm), without the need for complicated equipment. It supports different parts such as the armpits, limbs and around the lips. The treatment area can be adapted by adjusting the coverage range of the light spot (diameter 5-8cm), and the operation is highly flexible.

[0045] Breaking through the limitations of traditional laser hair removal which relies on melanin, this technology establishes for the first time a PDT hair removal framework of "photosensitizer follicle targeting + visible light activation of ROS". It covers all population groups in terms of mechanism of action, and solves the problem of high skin irritation in traditional PDT therapy by combining "low concentration photosensitizer + gentle light intensity". This is the first application of PDT technology in the field of hair removal.

[0046] Example 2: TCSVP-C4 in this technology is a photosensitizer with aggregation-induced emission properties. With advancements in research, various photosensitizers with similar properties can be used as alternatives. Some potential alternatives are listed below: Materials in the same series, such as TCSVP, TCSVP-C2, and TCSVP-C3, differ from TCSVP-C4 only in the length of the alkyl chain and have similar properties. TPE-QN / TPE-DQN: It has a tetraphenylphenyl fused quinine structure, is mitochondrial targeted, emits at a wavelength of 650nm, and has a stronger ability to generate 1O2; SIN (BZT-triphenylamine): is an AIE photosensitizer based on the D–A structure, possessing near-infrared emission, high fluorescence enhancement, high 1O2 yield, and natural lysosomal targeting.

[0047] Photosensitizers selected include, but are not limited to, aggregation-induced emission (AIE) photosensitizers, porphyrin photosensitizers, phthalocyanine photosensitizers, as well as RB, MB, ICG, ce6, and their photosensitive derivatives. The photosensitizers are in solution form, with solvents including, but not limited to, DMSO, hydrogel substrates, and ethanol solutions. The photosensitizer concentration is controlled within the range of 1-50 mmol / L, which is within the safe and effective concentration range for activating ROS.

[0048] Example 3: In addition to medical cooling gel, other physical / chemical cooling methods can also be used.

[0049] ①Physical cooling methods: Alcohol spray: This method uses the volatile nature of alcohol and the heat dissipation from evaporation to lower skin temperature. Ice packs / ice masks: simple, inexpensive, and provide direct and rapid cooling; Cold air jet: Fans, air coolers, etc. can be used to directly blow air onto the treatment area to increase the airflow on the skin surface and lower the skin temperature; ② Chemical cooling methods: Ultrasonic coupling gel: Its composition is similar to that of medical cryogel (mainly water with added thickeners), and it has good light transmittance and safety. Glycerin solution: Its refractive index is close to that of skin, which can reduce light scattering and has a cooling effect.

[0050] Example 4: Drug delivery can also take the following forms: Hydrogel base: TCSVP-C4 is embedded in the hydrogel base, applied for 30 minutes, followed by direct light exposure for 8 minutes, and then the treated area is thoroughly cleansed with a non-irritating cleanser. Microneedle delivery: TCSVP-C4 is loaded into soluble microneedles, which directly penetrate the stratum corneum to deliver the drug to the deeper layers of the superficial dermis / hair follicles. After dissolving, the skin is directly exposed to light for 8 minutes, and then the treated area is thoroughly cleansed with a non-irritating cleanser. Disposable patch / spray application: Apply medication using a disposable patch / spray, wipe off the medication after 5 minutes, wait 20-30 minutes, then expose to light for 8 minutes, and thoroughly cleanse the treated area with a non-irritating cleanser.

[0051] Example 5: The treatment course can be adjusted according to the patient's skin condition: Short intervals (0, 3, 7 days): If the patient's skin condition is good and can tolerate the treatment, a short course of treatment can be selected, which is conducive to rapidly inducing hair follicle cell apoptosis; Medium intervals (0, 10, 20 days): allow for skin repair and may be more suitable for most patients.

[0052] Long intervals (once every two weeks, for a total of 4–8 sessions): Similar to the long-term strategy of laser hair removal to cover hair growth, resulting in more stable long-term effects.

[0053] Example 6: This invention adjusts technical parameters for different scenarios, including but not limited to: Suitable for home use: Utilizes a combination of soluble microneedles loaded with photosensitizer and commercially available portable LED light source (380-460nm, 30-40mW / cm²). 2 After the microneedles penetrate the stratum corneum, they dissolve and release photosensitizers. The light exposure time is 10-15 minutes, and no professional operation is required. Suitable for light-colored hair: Add 0.1-0.5% polydopamine nanoparticles (melanin mimic) to the photosensitizer solution to enhance the absorption of light energy by hair follicles and extend the light exposure time to 12-15 minutes to ensure hair removal effect on light-colored hair. Suitable for large-area hair removal scenarios: Utilizes "photosensitizer spray application + large-area LED panel light source (light intensity 45-50mW / cm²)". 2 "Spot diameter ≥ 10cm", single irradiation coverage 100-200cm 2 In areas that improve treatment efficiency.

[0054] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A hair removal method based on aggregation-induced emission (AIE) materials for oxidative stress, characterized in that: The specific operating steps include the following: S1. Pre-treat the skin in the area requiring hair removal; S2. Apply TCSVP-C4 to the treated area of ​​skin, leave it on, then wipe it off and let it air dry; S3. Photoactivation treatment of the skin surface; S4. Postoperative cleaning, care and intensive treatment.

2. The oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials according to claim 1, characterized in that: The specific process for pre-treating the skin in the area to be treated in step 1 is as follows: thoroughly clean the skin in the treatment area with water or a non-irritating cleanser to remove oil and dirt from the skin surface; for areas with longer hair, shave the hair on the surface of the treatment area with a disposable safety razor, leaving 0.5-1mm stubble to facilitate TCSVP-C4 contact with the hair follicle opening.

3. The oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials according to claim 1, characterized in that: The specific procedure for applying TCSVP-C4 to the skin of the treatment area in step 2, leaving it to stand, wiping it off, and letting it air dry is as follows: 1) Use a sterile applicator to evenly apply TCSVP-C4 to the treatment area, with a thickness of 0.1-0.2 mm, and the coverage area extends 1-2 cm beyond the target area. The concentration of TCSVP-C4 is 10 mmol / L, and the solvent is DMSO. 2) Let it sit in a dark place for 5 minutes to allow the material to fully penetrate into the hair follicle opening and the superficial dermis. Avoid strong light exposure during this period. 3) Soak a cotton cloth or gauze in 70% alcohol and gently wipe away the surface material. Then wait 10-20 minutes in a dark place to let the skin dry.

4. The oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials according to claim 1, characterized in that: The specific process for photoactivation treatment of the skin surface in step 3 is as follows: 1) Apply medical cooling gel to the skin surface to lower skin temperature; 2) Activate the customized light source equipment, adjust the light spot coverage of the treatment area, and set the parameters as follows: light intensity 45mW / cm². 2 Irradiate for 5-10 minutes, depending on the patient's skin sensitivity; monitor skin temperature in real time during irradiation, and control the skin surface temperature to ≤40℃ to avoid burns. If the temperature exceeds the threshold, irradiation should be stopped.

5. The oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials according to claim 1, characterized in that: The specific procedures for postoperative cleaning, nursing care, and intensive treatment in step 4 are as follows: 1) Postoperative cleaning: Use a non-irritating cleanser to thoroughly clean the treated area to prevent material residue from damaging the skin if left for too long; 2) Postoperative care: Apply aloe vera gel and moisturizer to the treated area to moisturize the skin. 6.3) Treatment course: Repeat the treatment on the 4th and 10th day after the first treatment, for a total of 3 treatments, to form a continuous regulation of hair follicles and achieve long-term hair removal effect.

7. The oxidative stress-related hair removal method based on aggregation-induced emission (AIE) materials according to claim 4, characterized in that: The light source is a Fenix ​​E28R V2.0 flashlight, operating at low brightness with a brightness of 60 lumens and an actual luminous intensity of 45 mW / cm². 2 The irradiation distance is fixed at 25cm.

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