Preparation method of trillium tschonoskii carbon quantum dots and application of trillium tschonoskii carbon quantum dots in cosmetics
The preparation of Trillium carbon quantum dots by high-temperature carbonization solves the problems of expensive preparation methods and limitations of traditional Chinese medicine extracts in existing technologies, enabling safe and effective cosmetic applications and improving the bioavailability and skin care effects of Trillium in cosmetics.
Patent Information
- Application Number
- CN202511659696.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-13
- Publication Date
- 2026-02-03
AI Technical Summary
Existing methods for preparing carbon quantum dots are expensive and complex, and traditional Chinese medicine extracts suffer from problems such as solvent residue, toxicity, and low component utilization, which limit their application in cosmetics.
Trillium carbon quantum dots were prepared by high-temperature carbonization. Using Trillium rhizomes as raw materials, the active ingredients were preserved and organic solvents and toxicity were avoided through simple steps such as drying, calcination, ultrasonication and freeze-drying, resulting in uniform nano-sized particles.
The resulting Trillium carbon quantum dots are safe and non-toxic, easily soluble in water, and have improved bioavailability. They offer multiple skincare benefits, such as promoting cell vitality, moisturizing, anti-inflammatory, anti-wrinkle, and antioxidant effects, making them suitable for large-scale commercial applications.
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Figure CN121449049A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of cosmetic raw material technology, specifically relating to a method for preparing carbon quantum dots from Trillium truncata and their application in cosmetics. Background Technology
[0002] Carbon quantum dots (CDs) are carbon nanoparticles with high dispersion and a size of less than 10 nm. CDs possess unique photoluminescence properties, excellent water solubility, low cytotoxicity, and good thermal stability, thus showing broad application prospects in photocatalysis, bioimaging, free radical scavenging, and analytical sensing. Biomass CDs refer to CDs directly converted from natural biomass. Compared with chemically synthesized CDs, they have advantages such as environmental friendliness, economy, and readily available materials, while avoiding toxic precursors and complex post-processing steps. Various natural biomass materials have been used as precursors for CD preparation, such as garlic, peanuts, frangipani, watermelon rind, green peppers, and onions. Biomass is chosen as a precursor because these materials contain abundant organic compounds, and some surface-active groups are retained after carbonization. For example, studies by Bodhisatwa Das et al. have shown that CDs derived from jujube molasses are rich in active groups such as hydroxyl, aldehyde, and ketone groups, making them ideal reactive oxygen species scavengers. Currently, methods for synthesizing CDs using natural biomass mainly include hydrothermal / solvothermal methods, microwave synthesis, arc discharge methods, laser ablation methods, ultrasonic methods, and ball milling methods. However, these methods usually require expensive equipment or complex processing, which is not conducive to the commercial application of bio-based CDs. Developing economical and simple methods for CD preparation remains a significant challenge.
[0003] Traditional Chinese medicine often suffers from large volume, low bioavailability, and side effects, preventing it from fully exerting its medicinal effects. Carbon quantum dots, however, possess excellent biocompatibility, low toxicity, stable physicochemical properties, ultra-small size, and abundant active groups, significantly improving bioavailability. Trillium, a plant belonging to the genus Trillium in the family Liliaceae, is also known as "the pearl on the head." Both its rhizomes and mature fruits have certain medicinal value and are recorded in literature such as the *Dictionary of Traditional Chinese Medicine*, *National Compendium of Chinese Herbal Medicine*, *Seven Herbs of Taibai*, and *Flora of Qinling*. The chemical components of Trillium mainly include steroidal saponins, flavonoids, fatty acids, and polysaccharides. However, there are some limitations to the application of trillium extract in cosmetics: First, organic solvents are usually used in the preparation process, which may result in the extract being contaminated with organic solvents; second, the polarity and water solubility of saponins in each prepared trillium extract vary greatly, making them difficult to apply in cosmetics; third, the saponins in trillium extract may exhibit varying degrees of toxicity; and finally, the current extraction process only extracts a portion of the components, while most of the components are discarded.
[0004] The aforementioned existing technologies reflect the current level of development in the field of carbon quantum dots (CDs), including their unique optical and physicochemical properties and their potential applications in multiple fields. These properties give CDs significant advantages as active ingredients in cosmetics, but the lack of economy and simplicity in existing preparation methods has become a limiting factor. Biomass-derived CDs, by retaining surface-active groups, as exemplified by examples from Bodhisatwa Das et al., have demonstrated their potential in scavenging reactive oxygen species, providing inspiration for developing similar materials from traditional Chinese medicinal herbs such as Trillium frutescens. However, the limitations of traditional Chinese medicine extraction, including solvent residues and toxicity risks, further highlight the necessity of converting Chinese medicine into nanoscale CDs. This conversion can not only improve bioavailability but also reduce side effects, ensuring safety in cosmetic applications. Trillium frutescens, a plant included in multiple Chinese medicine literatures, provides abundant precursors for CD synthesis due to the diversity of its rhizome components, but current technologies have not yet explored its specific carbonization pathway. Summary of the Invention
[0005] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a method for preparing carbon quantum dots from Trillium oxypetalum.
[0006] Another object of the present invention is to provide the application of the Trillium carbon quantum dots prepared by the above preparation method in cosmetics.
[0007] The technical solution of the present invention is as follows:
[0008] A method for preparing carbon quantum dots from Trillium oxypetalum includes the following steps:
[0009] (1) The rhizomes of Trillium are washed, dried, pulverized into fine powder and sieved to obtain Trillium rhizome powder;
[0010] (2) The root and stem powder of Trillium truncatum was calcined in air at 150℃~200℃ for 2~5 h to obtain carbonized Trillium truncatum powder;
[0011] (3) After mixing the carbonized Trillium powder obtained in step (2) with pure water, it is dissolved by ultrasonication and then filtered with the first microporous membrane to obtain a clear liquid;
[0012] (4) The clear liquid obtained in step (3) is filtered through a second microporous membrane to obtain a clear brown solution;
[0013] (5) Freeze-dry the brown clear solution obtained in step (4) to obtain Trillium carbon quantum dot powder.
[0014] In a preferred embodiment of the present invention, in step (1), the drying temperature is 60 °C, the time is 20-25 h, and the sieving is through an 80-200 mesh sieve.
[0015] In a preferred embodiment of the present invention, in step (3), the mass ratio of the carbonized Trillium powder to pure water is 1:50-100, the ultrasonic dissolution temperature is 18-22℃, the frequency is 20-40 kHZ, and the time is 0.5-2h.
[0016] More preferably, in step (3), the pore size of the first microporous filter membrane is 0.45 μm.
[0017] In a preferred embodiment of the present invention, in step (4), the pore size of the second microporous filter membrane is 0.22 μm.
[0018] In a preferred embodiment of the present invention, in step (4), the freeze-drying time is 24-36 hours.
[0019] Application of Trillium carbon quantum dots prepared by the above method in the preparation of cosmetic compositions.
[0020] In a preferred embodiment of the present invention, the cosmetic composition is a toner or a face cream.
[0021] A cosmetic composition comprising Trillium carbon quantum dots prepared by the above method.
[0022] In a preferred embodiment of the present invention, it is a toner or face cream.
[0023] The beneficial effects of this invention are:
[0024] 1. This invention uses a high-temperature carbonization method to prepare Trillium carbon quantum dots. It uses the whole rhizome of Trillium, which effectively preserves all the active ingredient groups of Trillium rhizome, including steroidal saponins, flavonoids, polysaccharides, etc., avoiding the loss of active ingredients that may be caused by traditional extraction methods, thereby maximizing the utilization of plant resources and achieving efficient nanoscale conversion.
[0025] 2. The carbon quantum dots of Trillium prepared by this invention are free of organic solvents and have no cytotoxicity. They are easily soluble in water, which reduces the inherent toxicity of Trillium and ensures its safety for skin application. Experiments have shown that the viability of RAW264.7, HaCaT and HFF cells exceeds 90% at different concentrations, and promotes cell growth at appropriate concentrations, demonstrating good biocompatibility.
[0026] 3. The carbon quantum dots of Trillium prepared by this invention are more natural and will not cause any side effects to the skin. In addition, their particle size is at the nanoscale, making them easier to be absorbed by the skin. TEM observation shows that they are uniformly dispersed spherical nanoparticles without obvious aggregation, which further enhances their permeability and efficacy in skin care compositions.
[0027] 4. This invention does not require expensive equipment, and the operation steps are simple and the cost is lower, making it suitable for the production of cosmetic raw materials. The entire process only involves conventional operations such as drying, calcination, ultrasonication, and freeze-drying, avoiding complex post-processing and making it suitable for large-scale commercial applications.
[0028] 5. The trillium carbon quantum dots prepared by this invention have multiple skin care benefits, including promoting the secretion of AQP3 in HaCaT cells to achieve moisturizing, inhibiting the release of NO in RAW264.7 cells to achieve anti-inflammatory and soothing effects, increasing the level of type I collagen in HFF cells to achieve anti-wrinkle and firming effects, and having a DPPH free radical scavenging rate of over 70% to achieve antioxidant effects. These benefits have been verified through cell models and in vitro experiments and are significantly superior to traditional trillium extracts.
[0029] 6. In practical cosmetic applications, such as toners and face creams, consumer tests show that after 28 days of use, the skin's moisturizing, firming, and anti-wrinkle effects are significantly improved, demonstrating the practicality and market potential of this invention and further enriching the selection of green cosmetic raw materials. Attached Figure Description
[0030] Figure 1 This shows a TEM transmission electron microscope image of carbon quantum dots from Trillium truncatum in Example 1 of the present invention.
[0031] Figure 2 (a) Showing the effect of different concentrations of Trillium truncatum carbon quantum dot samples on the viability of RAW264.7 cells in Example 1 of the present invention. Figure 2 (b) Showing the effect of different concentrations of Trillium carbon quantum dot samples on Hacat cell viability in Example 1 of the present invention. Figure 2 (c) Shows the effect of different concentrations of Trillium carbon quantum dot samples on HFF cell viability in Example 1 of the present invention.
[0032] Figure 3 This invention demonstrates the effect of Trillium carbon quantum dots in Example 1 on AQP3 content in Hacat cells.
[0033] Figure 4 This invention demonstrates the effect of Trillium carbon quantum dots in Example 1 on NO release from RAW264.7 cells.
[0034] Figure 5 This invention demonstrates the effect of Trillium carbon quantum dots in Example 1 on the release of type I collagen from HFF cells.
[0035] Figure 6 This invention demonstrates the effect of Trillium carbon quantum dots in Example 1 on DPPH free radical scavenging rate.
[0036] Figure 7This displays the consumer usage test results of the toner in Embodiment 2 of the present invention.
[0037] Figure 8 This shows the results of consumer use tests of the face cream in Example 3 of the present invention. Detailed Implementation
[0038] The technical solution of the present invention will be further explained and described below with reference to specific embodiments and accompanying drawings.
[0039] Example 1
[0040] (1) After washing the roots and stems of Trillium, dry them in an electric heating drying oven at 60℃ for 24 h, then pulverize them into fine powder and sieve them (100 mesh).
[0041] (2) Weigh an appropriate amount of the Trillium root powder from step (1) and put it into a crucible. Calcinate it in air at 200°C for 2 h to obtain carbonized Trillium powder.
[0042] (3) Prepare the mixture according to the mass ratio of carbonized Trillium powder to pure water of 1:100, and then sonicate it in an ultrasonic machine at 20℃ and 40KHz for 1 h.
[0043] (4) After the ultrasound was completed, the supernatant was collected by centrifugation using a microporous membrane with a pore size of 0.45 μm, and then filtered by suction using a microporous membrane with a pore size of 0.22 μm to obtain a clear brown solution.
[0044] (5) Place the brown clear solution from step (4) into a freeze dryer and dry for 36 h to obtain Trillium carbon quantum dot powder, and store it after drying.
[0045] To verify the safety and efficacy of the Trillium carbon quantum dots prepared in this embodiment, the following experiments were conducted, and the experimental results were analyzed and discussed in detail:
[0046] 1. Materials and Methods
[0047] Cell culture: HaCaT and HFF cells, RAW264.7 were purchased, revived, and cultured at 37 ℃ and 5% CO2 to a suitable density. The culture medium was discarded, the residual culture medium was washed with PBS, and the cells were digested with trypsin. The digestion was stopped by adding an equal volume of FBS to the trypsin. Twice the volume of PBS was added, and the cell suspension was collected by pipetting. The cells were centrifuged at 1500 rpm for 5 min, resuspended in complete culture medium, and passaged. Cells in the logarithmic growth phase and in good condition were used for the next experiment.
[0048] CCK assay for cell viability: The experiment included a negative control group and a sample group. Six concentration gradient groups were designed for the sample group, with concentrations from high to low: C1, C2, C3, C4, C5, and C6, with three parallel wells per group. Cells in the logarithmic growth phase and in good condition were digested, centrifuged, resuspended in the appropriate cell culture medium, and counted. Cell suspensions of specific concentrations were prepared according to different cell sizes, culture experience, and experimental requirements. 95 μL of cell suspension was added to each well of a 96-well plate (empirical value: HaCaT cell count 3 × 10⁻⁶). 4 / well, RAW264.7 cells, 4×10⁶ 4 / well, HFF cells 3×10 4 / well), and cultured overnight at 37 ℃ and 5% CO2 for (14±2) h. Samples were added (5 μL of PBS was added to each well of the negative control group, and 5 μL of the corresponding concentration of sample was added to each well of the sample group). The cells were cultured at 37 ℃ and 5% CO2 for 24 h. CCK reagent was added according to the CCK kit instructions and the absorbance was measured to calculate cell viability.
[0049] Hacat cell hydration activity assay: Hydration model – Digested cells in the logarithmic growth phase and in good condition, centrifuged, and resuspended in appropriate cell culture medium. Cells were counted to prepare a cell suspension of a specific concentration. Experimental design: blank group, drying group, and experimental group, three parallel wells per group. Cell suspension was seeded into each well of a 48-well plate and cultured at 37℃, 5% CO2 for 24 h. The culture medium was discarded, and the cells were washed. Culture medium and PBS were added to each well of the blank and drying groups, while culture medium and Trillium truncatum carbon quantum dots were added to each well of the experimental group. The cells were cultured at 37℃, 5% CO2 for 24 h. The cells of the drying and experimental groups were placed in a laminar flow hood and dried at a fan speed of 0.31 m / s, and cultured at 37℃, 5% CO2 for another 24 h. The cell supernatant was collected, and the AQP3 content was measured and calculated according to the ELISA kit instructions.
[0050] RAW264.7 Cell Anti-inflammatory Activity Detection: Cells in the logarithmic growth phase and in good condition were digested, centrifuged, resuspended in appropriate cell culture medium, counted, and prepared into a cell suspension of a certain concentration. The experiment was designed with a blank group (PBS group), a control group (LPS group), and a sample group, with 3 parallel wells in each group. 200 μL of cell suspension was seeded into each well of a 48-well plate and cultured overnight at 37℃ and 5% CO2 for (14±2) h. The culture medium was discarded, and the cells were washed with 200 μL / well of physiological saline or PBS. In the blank control group, 195 μL of culture medium and 5 μL of PBS were added to each well. In the control group, 191 μL of culture medium, 4 μL of LPS working solution (final LPS concentration of 1 μg / mL), and 5 μL of PBS were added to each well. In the sample group, 191 μL of culture medium, 4 μL of LPS working solution (final LPS concentration of 1 μg / mL), and 5 μL of the sample to be tested were added to each well. The cells were cultured at 37 ℃ and 5% CO2 for 24 h. Cell morphology was observed, cell supernatant was collected, and NO content was detected and calculated according to the kit instructions.
[0051] HFF cell assay for anti-wrinkle and firming activity: Cells in the logarithmic growth phase and in good condition were digested, centrifuged, resuspended in appropriate cell culture medium, and counted to prepare a cell suspension of a specific concentration. The experiment included a blank group (PBS group), a control group (LPS group), and a sample group, with three parallel wells in each group. 2 mL of cell suspension was seeded into each well of a 6-well plate and cultured overnight at 37℃ and 5% CO2 for (14±2) h. The culture medium was discarded, and the cells were washed with 2 mL / well of physiological saline or PBS. 2 mL of physiological saline or PBS was added to each well. The negative control group did not receive UV irradiation treatment. The irradiated group and the sample group were irradiated with a UV lamp (irradiation dose based on UVA, which can be referenced as 0.35 J / cm²). 2 After irradiation, the culture medium was replaced, and a non-toxic concentration of sample was added according to the cytotoxicity results. After sample addition, the cells were cultured at 37 ℃ in a 5% CO2 incubator for one day (24 h). 200 μL of cell culture supernatant was collected in a sterile EP tube, and the content of type I collagen in the supernatant was detected according to the instructions of the human type I collagen (COL1) enzyme-linked immunosorbent assay kit (tested immediately upon collection).
[0052] DPPH free radical scavenging ability detection: Trillium carbon quantum dots were dissolved in pure water, and several different concentration gradients were set up. Using the ELISA kit for DPPH free radical scavenging ability detection, 80 μL of sample and 120 μL of reagent-1 were added to the test tube, 80 μL of sample and 120 μL of extract were added to the control tube, and no sample was added to the blank tube, only 80 μL of extract and 120 μL of reagent-1 were added. After mixing, the mixture was reacted at room temperature in the dark for 30 min, and the absorbance value A was measured at a wavelength of 515 nm.
[0053] DPPH free radical scavenging rate (%) = (1 - [(A assay - A control) ÷ A blank)] × 100%
[0054] 2. Experimental Results:
[0055] The morphology of *Trillium truncatum* carbon quantum dot particles was studied using TEM (transmission electron microscopy). The *Trillium truncatum* carbon quantum dots were found to be uniformly dispersed spherical nanoparticles without obvious aggregation (e.g., ...). Figure 1 (As shown).
[0056] Cell viability—To ensure the reliability of subsequent experimental data, this embodiment used a CCK experiment to detect the effect of different concentrations of Trillium carbon quantum dots on cell viability before investigating the efficacy of Trillium carbon quantum dots. The results are as follows: Figure 2 As shown, the carbon quantum dots of Trillium did not exhibit cytotoxicity to RAW264.7 cells, HaCaT cells, and HFF cells at different concentrations, and the cell viability was above 90%. Furthermore, at appropriate concentrations, the carbon quantum dots of Trillium also promoted cell growth.
[0057] Moisturizing – Aquaporin 3 (AQP3) is a complete membrane protein that penetrates biological membranes to form water channels. It is primarily responsible for assisting in the transport of small molecules such as water and glycerol to different layers of skin tissue and is closely related to epidermal hydration. Numerous studies have shown that AQP3 is the most abundant AQP subtype in the skin and has the strongest correlation with skin hydration. This example selects AQP3 as an evaluation index to explore the moisturizing effect of Trillium coronarium carbon quantum dots. Figure 3 It is known that the carbon quantum dots of Trillium can effectively promote the secretion of AQP3 by HaCaT cells, thus having a moisturizing effect.
[0058] Anti-inflammatory and soothing effects—Inflammation is the body's defensive response to harmful factors, involving multiple cells and accompanied by the release of inflammatory mediators. Nitric oxide is often used as an indicator of inflammation and a target for alleviating it. During the inflammatory response, nitric oxide synthase (iNOS) is activated, producing high concentrations of NO, which activates the NF-κB signaling pathway, further inducing the release of pro-inflammatory factors and exacerbating the inflammatory response. Therefore, inhibiting the production of iNOS and NO is one way to alleviate inflammation.
[0059] This embodiment utilizes an LPS-induced RAW264.7 cell model, selecting NO as an evaluation index to investigate the anti-inflammatory activity of Trillium commune carbon quantum dots, thereby evaluating the potential soothing efficacy of this active ingredient. In this embodiment, cell supernatant was collected to detect the inflammatory mediator NO. For example... Figure 4 As shown, in the LPS-activated RAW264.7 cell experiment, the NO content increased significantly after LPS activation; after 24 h of treatment with Trillium carbon quantum dots, the NO content decreased significantly, indicating that Trillium carbon quantum dots have significant anti-inflammatory effects.
[0060] Anti-wrinkle and firming effects – Ultraviolet (UV) radiation has strong penetrating power and radiant energy. When it acts on the skin, it causes collagen to become distorted and broken, leading to wrinkles, reduced elasticity, moisture loss, and pigmentation. It is one of the most significant external factors contributing to skin aging. Type I collagen is a major component of the extracellular matrix of dermal cells. Its content decreases after exposure to UV radiation, resulting in loose skin and wrinkles. Human dermal fibroblasts can serve as a cell model for studying how cosmetics can increase type I collagen levels. By measuring changes in type I collagen levels among different groups after administration of the test substance, the efficacy of the test substance in influencing collagen levels can be evaluated.
[0061] This embodiment utilizes ultraviolet irradiation to induce HFF cell damage and evaluates the anti-wrinkle and firming effects by comparing type I collagen levels. Figure 5 As shown, the level of type I collagen decreased significantly after ultraviolet (UV) irradiation, but increased significantly after 24 hours of treatment with Trillium carbon quantum dots. This indicates that Trillium carbon quantum dots have a firming effect.
[0062] Antioxidant properties—DPPH scavenging rate is one of the important indicators for measuring the antioxidant performance of a substance. A high scavenging rate indicates that the substance has a strong antioxidant capacity and can more effectively protect cells from oxidative stress damage. For example... Figure 6 As shown, in this embodiment, the DPPH scavenging rate of Trillium carbon quantum dots increases with increasing dosage. When the concentration of Trillium carbon quantum dots is 10 mg / ml, the DPPH scavenging rate is higher than 70%, so Trillium carbon quantum dots have a good antioxidant effect.
[0063] The above experiments have confirmed that the carbon quantum dots of Trillium prepared in this embodiment can promote the production and expression of AQP3 in HaCaT cells, inhibit the release of inflammatory mediators from RAW264.7 cells, have a significant inhibitory effect on NO, protect HFF cells from ultraviolet damage, reduce collagen loss, have anti-wrinkle and firming effects, and neutralize DPPH free radicals, thus having an antioxidant effect.
[0064] In summary, the Trillium carbon quantum dots prepared in this embodiment have good moisturizing, soothing, anti-wrinkle, firming, and antioxidant effects.
[0065] Example 2
[0066] A toner comprising:
[0067] Carrier components: 5 wt% glycerol, 3 wt% butylene glycol, 86 wt% deionized water;
[0068] Active ingredient: 6 wt% of Trillium carbon quantum dots prepared in Example 1.
[0069] The preparation method is as follows: Weigh out glycerol, butanediol and deionized water, add them to a water pot and heat to 95°C. After stirring and mixing, cool to room temperature. Then, while stirring, add the Trillium carbon quantum dots prepared in Example 1 until the mixture is uniform.
[0070] A volunteer usage test was conducted with 35 participants. Each participant used the sample twice daily, morning and evening (without using other face creams, serums, or lotions) for 28 days. Questionnaires were completed twice, on days 14 and 28. The consumer usage test results for the toner prepared in this embodiment are as follows: Figure 7 As shown.
[0071] Example 3
[0072] A face cream comprising:
[0073] Carrier components: 1,3-Butanediol 3 wt%, Glycerin 6 wt%, Deionized water 72.5 wt%, Ammonium Acryloyldimethyltaurate / VP copolymer 0.5 wt%, Caprylic / Capric triglyceride 5 wt%, Macadamia seed oil 4 wt%, Cetearyl alcohol oleate / Sorbitan oleate 4 wt%;
[0074] Active ingredient: 5 wt% of Trillium carbon quantum dots prepared in Example 1.
[0075] The preparation method is as follows: The weighed A phase component (glycerol, 1,3-butanediol, deionized water, ammonium acryloyldimethyl taurate / VP copolymer) is added to a water pot and heated to 95°C, and stirred until homogeneous; the B phase component (caprylic / capric triglyceride, macadamia seed oil, cetearyl oleate / sorbitan oleate) is heated to 80°C; the B phase component is added to the A phase component and homogenized and emulsified, cooled to room temperature, and the Trillium carbon quantum dots prepared in Example 1 are added while stirring until the mixture is homogeneous.
[0076] The consumer use test results of the face cream prepared in this embodiment are as follows: Figure 8 As shown.
[0077] The above description is merely a preferred embodiment of the present invention, and therefore should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made in accordance with the scope of the patent and the contents of the specification should still fall within the scope of the present invention.
Claims
1. A method for preparing carbon quantum dots from Trillium truncata, characterized in that: Includes the following steps: (1) The rhizomes of Trillium are washed, dried, pulverized into fine powder and sieved to obtain Trillium rhizome powder; (2) The root and stem powder of Trillium truncatum was calcined in air at 150℃~200℃ for 2~5 h to obtain carbonized Trillium truncatum powder; (3) After mixing the carbonized Trillium powder obtained in step (2) with pure water, it is dissolved by ultrasonication and then filtered with the first microporous membrane to obtain a clear liquid; (4) The clear liquid obtained in step (3) is filtered through a second microporous membrane to obtain a clear brown solution; (5) Freeze-dry the brown clear solution obtained in step (4) to obtain Trillium carbon quantum dot powder.
2. The preparation method according to claim 1, characterized in that: In step (1), the drying temperature is 60 ℃, the time is 20-25 h, and the sieve is 80-200 mesh.
3. The preparation method according to claim 1, characterized in that: In step (3), the mass ratio of the carbonized Trillium powder to pure water is 1:50-100, the ultrasonic dissolution temperature is 18-22℃, the frequency is 20-40 kHZ, and the time is 0.5-2h.
4. The preparation method according to claim 3, characterized in that: In step (3), the pore size of the first microporous filter membrane is 0.45 μm.
5. The preparation method according to claim 1, characterized in that: In step (4), the pore size of the second microporous filter membrane is 0.22 μm.
6. The preparation method according to claim 1, characterized in that: In step (4), the freeze-drying time is 24-36 hours.
7. The use of Trillium carbon quantum dots prepared by the preparation method according to any one of claims 1 to 6 in the preparation of cosmetic compositions.
8. The application as described in claim 7, characterized in that: The cosmetic composition is a toner or face cream.
9. A cosmetic composition, characterized in that: It contains Trillium carbon quantum dots prepared by the preparation method described in any one of claims 1 to 6.
10. A cosmetic composition as described in claim 9, characterized in that: It is either a toner or a face cream.