Animal cell immunocompetence analyzer and analysis method

Abstract

The present invention relates to the technical field of cell analyzers, and discloses an animal cell immunocompetence analyzer, which comprises: a housing, the upper end of which is provided with a display operation screen, the front end of which is provided with a reagent plate docking port, the reagent plate docking port is internally provided with a telescopic reagent plate receiving member for receiving an animal cell reagent plate, the side wall of which is provided with a discharge port, and the side wall of which is correspondingly provided with a collection box; the coaxial integrated bias analysis area is arranged in the shell, and a central positioning column vertically extending is arranged in the center of the coaxial integrated bias analysis area. According to the invention, through the circumferential layout of the coaxial integrated bias analysis area and the equidistant distribution of the plurality of independent incubation units, synchronous incubation and detection of multiple samples are realized; the movable inner cores are accurately conveyed through the transfer conveying piece and the conveying separation piece, the single-time operation efficiency is improved, and the efficiency bottleneck of serial processing of traditional equipment is solved.

Description

Technical Field

[0001] This invention relates to the field of cell analyzer technology, specifically to an animal cell immune activity analyzer and analytical method. Background Technology

[0002] Traditional animal cell immunomodulators are primarily used to assess the killing ability or proliferative response of immune cells (such as T cells and NK cells) against target cells, and are widely applied in vaccine development, immunotherapy, and basic immunology research. These instruments are typically based on enzyme-linked immunosorbent assay (ELISA), lactate dehydrogenase (LDH) release assays, or fluorescence / chemiluminescence detection principles, indirectly reflecting cellular immune activity by measuring changes in specific signals within the reaction system. The operational process generally involves pre-filling animal cells and reagents into microplates, incubating them at a constant temperature for a certain period, and then reading the absorbance or fluorescence intensity of each well using an optical detection module. Finally, the immunomodulatory activity index is calculated using a standard curve.

[0003] However, traditional animal cell immunoassay analyzers with existing technology have significant limitations in practical applications. Their core structure often employs a single-channel or fixed detection platform, with only a single incubation chamber and a single detection site. This means that each run can only process one reagent plate, and even then, the plate often needs to be incubated and detected in batches. Because the incubation process requires strict temperature stability and a closed environment, most devices cannot load or detect other samples simultaneously; the next round of operations must wait until the current batch is completely finished. This sequential working mode severely restricts overall detection efficiency. Furthermore, the loading, positioning, and unloading of reagent plates largely rely on manual operation or semi-automatic robotic arms, which is not only time-consuming but also prone to introducing human error. Even some devices with automatic sample loading capabilities lack multi-station coordination capabilities in their internal transport mechanisms, making it impossible to achieve parallel processing of incubation and detection. Therefore, when facing large-scale sample screening or highly reproducible experiments, traditional analyzers often require a significant amount of time, failing to meet the demands of modern biomedical research for high efficiency, high reproducibility, and large-volume data output. Especially in veterinary vaccine efficacy evaluation or multi-condition immune function comparison experiments, low throughput has become a key bottleneck restricting experimental progress and data quality. Summary of the Invention

[0004] The purpose of this invention is to provide an animal cell immune activity analyzer to solve the problems mentioned in the background art.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution: an animal cell immune activity analyzer, comprising:

[0006] The housing has a display and operation screen at the top and a reagent plate interface at the front. The reagent plate interface has a retractable reagent plate receiver for receiving animal cell reagent plates. The side wall has an outlet and a corresponding collection box.

[0007] The coaxial integrated bias analysis area is located inside the housing, with a vertically extending central positioning post at its center. The components are integrated from top to bottom around this central positioning post.

[0008] Transfer conveying area: It is equipped with a transfer conveying component that can rotate and rise around the central positioning column. The transfer conveying component includes a first circumferential adjustment component and a transfer conveying body that is rotatably sleeved on the outer wall of the central positioning column through the first circumferential adjustment component.

[0009] High-throughput constant temperature incubation area: It is equipped with a constant temperature incubation component that is fixedly sleeved on the outer wall of the central positioning column. Multiple independent incubation units are evenly distributed in the circumferential direction inside the component. Each incubation unit is equipped with a movable inner core that can be raised and lowered.

[0010] Detection and separation area: It is equipped with a detection component that can rotate around the central positioning column and a conveying and separation component. The detection component is fixedly installed on one side of the circumference of the conveying and separation component.

[0011] Each component achieves high-throughput continuous processing through the linkage of the moving inner core. The moving inner core corresponds one-to-one with the incubation unit, and the carrier is transferred only through the transfer conveyor and the conveyor separation component.

[0012] According to the above technical solution, the reagent plate receiving component includes:

[0013] The hollow frame is horizontally telescopically positioned inside the reagent plate interface via a movable component. Its side wall is provided with a first slider that is slidably connected to the first groove on the side wall of the reagent plate interface.

[0014] The moving component includes:

[0015] The first servo motor is fixed to the side wall of the reagent plate interface;

[0016] The first lead screw is coaxially connected to the output end of the first servo motor, and its thread passes through the screw hole on the side wall of the hollow frame;

[0017] A mirror-symmetrical clamping plate is movably mounted within a hollow frame via a spacing adjustment component, and the inner side of the clamping plate is provided with a rubber anti-slip layer.

[0018] According to the above technical solution, the spacing adjustment component includes:

[0019] A two-way lead screw is horizontally installed inside a hollow frame via a bearing housing;

[0020] The second slider has a vertically fixed clamping plate at the top and is slidably installed in the second slide groove at the top of the hollow frame, and is threadedly connected to a two-way lead screw.

[0021] The second servo motor is fixed at one end of the second slide groove, and its output end drives the bidirectional lead screw to rotate through a bevel gear set.

[0022] According to the above technical solution, the transfer and conveying component includes:

[0023] The transfer conveyor is rotatably fitted onto the outer wall of the central positioning column via the first circumferential adjustment component;

[0024] The detection component includes:

[0025] The telescopic base is rotatably fitted onto the outer wall of the central positioning column via the second circumferential adjustment component, and the conveying and separating component is fixedly installed at its front end;

[0026] The Z-axis slide rail is vertically mounted on the side of the telescopic base via a fixed bent rod.

[0027] Wavelength transmitter, slidably mounted on Z-axis slide rail.

[0028] According to the above technical solution, the second circumferential adjustment component has the same structure as the first circumferential adjustment component, including:

[0029] A hollow retaining ring is fixedly sleeved on the outer wall of the central positioning post, and the outer wall is provided with an annular toothed rack.

[0030] The third servo motor is fixed inside the telescopic base, and its output end gear meshes with a ring rack.

[0031] The guide rod is fixed at one end to the inside of the telescopic base, and the other end is equipped with a positioning ball, which is movably installed in the annular ball groove on the outer wall of the hollow fixing ring.

[0032] According to the above technical solution, the constant temperature incubation component includes:

[0033] A hollow constant temperature sleeve is fixedly fitted to the outer wall of the central positioning column, forming multiple independent constant temperature incubation spaces in the internal circumference.

[0034] Heating plates and temperature sensors are embedded in the inner walls of each constant-temperature incubation space;

[0035] The retractable sealing plate is movable and installed at the edge of the opening of the constant temperature incubation space via an electric telescopic cylinder;

[0036] The positioning bracket is fixed in the middle of the constant temperature incubation space by a radial fixing rod, and a third toothed rack is symmetrically arranged inside.

[0037] According to the above technical solution, the conveying separation component and the transfer conveyor body have the same structure, both including:

[0038] The C-shaped outer frame has vertical lifting grooves and a first rack on its inner walls on both sides;

[0039] The lifting inner frame has T-shaped sliders on both sides, which slide and engage in the lifting slide groove, and the second rack is symmetrically fixed on the inner wall;

[0040] The lifting motor is fixed to the side wall of the T-shaped slider, and the output end is provided with a first gear that meshes with a first rack.

[0041] The lifting inner frame has a docking protrusion at one end, which docks with the positioning groove of the positioning card seat;

[0042] The second rack and the third rack are joined together to form a continuous rack belt.

[0043] According to the above technical solution, the movable inner core includes:

[0044] The connector frame has a reagent plate slot at the front end.

[0045] The lifting assembly is mirror-symmetrically positioned on the side wall of the insertion frame;

[0046] The pushing component includes an electric push rod and a push plate. The electric push rod is fixed to the inner wall of the insertion frame, and the push plate slides against the bottom surface of the reagent plate slot.

[0047] According to the above technical solution, the lifting assembly includes:

[0048] The fourth servo motor is fixed in the embedded groove on the side wall of the plug frame;

[0049] The third gear is fixed to the output end of the fourth servo motor;

[0050] The third gear is meshed with the continuous rack belt.

[0051] The analytical method of the animal cell immunomodulator includes the following steps:

[0052] a. Set the incubation temperature, optical density threshold, and activity calculation parameters on the display screen;

[0053] b. The reagent plate receiving component receives the animal cell reagent plate, and the clamping plate fixes the animal cell reagent plate.

[0054] c. The transfer conveyor extracts the target moving inner core to the transfer conveyor area;

[0055] d. The reagent plate receiving component horizontally inserts the animal cell reagent plate into the reagent plate slot of the movable inner core;

[0056] e. The transfer conveyor returns the movable inner core containing the animal cell reagent plate to the original incubation unit;

[0057] f. The constant temperature incubator is incubated at the set temperature, and the optical density of the test piece is monitored in real time.

[0058] g. When the rate of change of optical density remains below the set threshold, the detection element is determined to have completed incubation.

[0059] h. The display screen calculates and displays the cellular immune activity index;

[0060] i. The conveying and separating component extracts the moving inner core, pushing the assembly out of the animal cell reagent plate;

[0061] j. The animal cell reagent plate is discharged through the reagent plate interface, and the moving inner core is reset.

[0062] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0063] (1) This device achieves simultaneous incubation and detection of multiple samples by using the circular layout of the coaxial integrated bias analysis area and the equidistant distribution of multiple independent incubation units. Each moving core is accurately transferred through the transfer and separation components, improving the efficiency of a single operation and solving the efficiency bottleneck of serial processing in traditional equipment.

[0064] (2) Through the overall layout of the coaxial integrated bias analysis area, the transfer and conveying area, the high-throughput constant temperature incubation area and the detection and separation area are vertically integrated around the central positioning column, which significantly shortens the physical distance between the functional modules, optimizes the flow path of the reagent plate carrier, thereby improving the overall processing efficiency, effectively reducing the equipment's footprint, and enhancing the structural compactness and space utilization.

[0065] (3) This device uses a movable inner core as the only carrier throughout the entire process. The carrier structure is not replaced in each stage of sample introduction, incubation, detection and unloading, which avoids the risk of sample misalignment, contamination or loss caused by multiple transfers. At the same time, it simplifies the control system logic and improves the reliability and repeatability of operation.

[0066] (4) The constant temperature incubation device has multiple independent constant temperature incubation spaces. Each space is equipped with an independent heating plate, temperature sensor and retractable sealing plate. It not only achieves precise temperature control and environmental isolation, but also supports parallel experiments under multiple conditions, which significantly improves the detection throughput and experimental flexibility, and ensures that the cell activity detection environment is undisturbed.

[0067] (5) The detection piece achieves precise focusing of the wavelength transmitter through the Z-axis slide rail. Combined with in-situ optical detection, the optical density measurement error is small. The wavelength transmitter can be flexibly positioned in both horizontal and vertical dimensions to achieve full coverage optical density scanning of all incubation wells, ensuring the integrity and real-time nature of the detection data, and supporting intelligent incubation endpoint determination based on dynamic change rate. Attached Figure Description

[0068] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings:

[0069] Figure 1 This is a first perspective view of the present invention;

[0070] Figure 2 This is a second perspective view of the present invention;

[0071] Figure 3 This is a third perspective view of the present invention;

[0072] Figure 4 This is a first partial three-dimensional schematic diagram of the present invention;

[0073] Figure 5 This is a second partial perspective view of the present invention;

[0074] Figure 6 This is a third partial perspective view of the present invention;

[0075] Figure 7 This is a fourth partial perspective view of the present invention;

[0076] Figure 8 This is a fifth partial perspective view of the present invention;

[0077] Figure 9 This is a sixth partial perspective view of the present invention;

[0078] Figure 10 This is a third-dimensional schematic diagram of the seventh part of the present invention;

[0079] Figure 11 This is the eighth partial perspective view of the present invention;

[0080] Figure 12 This is a third-dimensional schematic diagram of the ninth part of the present invention;

[0081] Figure 13 This is the present invention. Figure 5 A magnified view of a portion of point A in the middle;

[0082] In the diagram: 1-Shell, 2-Display and operation screen, 3-Reagent plate interface, 4-Coaxial integrated bias analysis area, 5-Central positioning column, 6-Transfer and conveying area, 7-Transfer and conveying component, 71-First circumferential adjustment assembly, 72-Transfer and conveying body, 721-C-shaped outer frame, 722-Vertical lifting slide, 723-First rack, 724-Lifting inner frame, 725-T-shaped slider, 726-Second rack, 727-Lifting motor, 728-First gear, 729-Mating protrusion, 8-Height 9-Constant temperature incubation zone, 91-Constant temperature incubation component, 92-Constant temperature incubation sleeve, 93-Heating plate, 94-Temperature sensor, 95-Retractable sealing plate, 96-Electric telescopic cylinder, 97-Positioning card holder, 971-Third rack, 972-Positioning groove, 98-Moving inner core, 981-Plug-in frame, 982-Reagent plate slot, 983-Lifting assembly, 9831-Fourth servo motor, 9832-Third gear, 984-Push assembly, 9841-Electric pusher Rod, 9842-Push plate, 10-Detection separation area, 11-Detection component, 111-Telescopic base, 1111-Z-direction slide rail, 1112-Fixed bent rod, 112-Second circumferential adjustment assembly, 1121-Hollow fixing ring, 1122-Annular rack, 1123-Third servo motor, 1124-Drive gear, 1125-Guide rod, 1126-Positioning ball, 1127-Annular ball groove, 113-Wavelength transmitter, 12-Conveying separation component, 13-Reagent plate receiver, 131 - Hollow frame, 1311- First slider, 1312- First slide groove, 132- Moving component, 1321- First servo motor, 1322- First lead screw, 133- Clamping plate, 1331- Rubber anti-slip layer, 134- Spacing adjustment component, 1341- Bidirectional lead screw, 1342- Bearing seat, 1343- Second slider, 1344- Second slide groove, 1345- Second servo motor, 1346- Bevel gear set, 14- Animal cell reagent plate, 15- Discharge port, 16- Collection box. Detailed Implementation

[0083] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0084] Please see Figure 1-13 The present invention provides a technical solution: an animal cell immune activity analyzer, comprising:

[0085] The housing 1 has a display and operation screen 2 at its upper end and a reagent plate interface 3 at its front end. The reagent plate interface 3 has a retractable reagent plate receiver 13 for receiving animal cell reagent plates 14. The side wall has an outlet 15 and a corresponding collection box 16.

[0086] The coaxial integrated bias analysis area 4 is located inside the housing 1, with a vertically extending central positioning post 5 at its center. The components are integrated from top to bottom with the central positioning post 5 as the axis.

[0087] Transfer conveying area 6: It is equipped with a transfer conveying component 7 that can rotate and rise around the central positioning column 5. The transfer conveying component 7 includes a first circumferential adjustment component 71 and a transfer conveying body 72 that is rotatably sleeved on the outer wall of the central positioning column 5 through the first circumferential adjustment component 71.

[0088] High-throughput constant temperature incubation area 8: It is equipped with a constant temperature incubation component 9 that is fixedly sleeved on the outer wall of the central positioning column 5. Multiple independent incubation units are evenly distributed in the circumferential direction inside the component, and each incubation unit is equipped with a movable inner core 98 that can be raised and lowered.

[0089] Detection separation zone 10: It is equipped with a detection element 11 that can rotate around the central positioning column 5 and a conveying separation element 12. The detection element 11 is fixedly disposed on one side of the circumferential direction of the conveying separation element 12.

[0090] Each component is linked by a movable inner core 98 to achieve high-throughput continuous processing. The movable inner core 98 corresponds one-to-one with the incubation unit and the carrier is transferred only through the transfer conveyor 7 and the conveyor separation component 12.

[0091] Specifically, the reagent plate receiver 13 includes:

[0092] The hollow frame 131 is horizontally telescopically mounted in the reagent plate interface 3 via the moving component 132. Its side wall is provided with a first slider 1311, which is slidably connected to the first sliding groove 1312 on the side wall of the reagent plate interface 3.

[0093] The moving component 132 includes:

[0094] The first servo motor 1321 is fixed to the side wall of the reagent plate interface 3;

[0095] The first lead screw 1322 is coaxially connected to the output end of the first servo motor 1321, and the thread passes through the screw hole on the side wall of the hollow frame 131;

[0096] A mirror-symmetrical clamping plate 133 is movably mounted in the hollow frame 131 via a spacing adjustment component 134, and a rubber anti-slip layer 1331 is provided on the inner side of the clamping plate 133.

[0097] The reagent plate receiver 13, as a key component at the front end of the animal cell immunoassay analyzer, is used to receive external reagent plates. It combines the functions of automatic docking, stable clamping, and precise guidance. The hollow frame 131 has an overall frame structure with internal space to accommodate the reagent plates. It can achieve horizontal telescopic movement through the moving component 132. The hollow frame 131 is installed inside the reagent plate interface 3. Its side wall is provided with a first slider 1311, which is connected to the first groove 13 on the side wall of the reagent plate interface 3. 12 forms a sliding fit relationship, thereby ensuring that the hollow frame 131 runs smoothly and accurately during horizontal extension and retraction, avoiding skewing or jamming. The moving component 132 is used to drive the hollow frame 131 to complete the extension and retraction actions. It includes a first servo motor 1321 and a first lead screw 1322. The first servo motor 1321 is fixedly installed on the side wall of the reagent plate interface 3, and its output end is coaxially connected to the first lead screw 1322. The first lead screw 1322 passes through a screw hole opened on the side wall of the hollow frame 131 and is connected to it. A threaded engagement is formed. When the first servo motor 1321 is started, it drives the first lead screw 1322 to rotate, which in turn pushes the hollow frame 131 to move horizontally along the direction of the first slide groove 1312 through the threaded transmission. This enables the reagent plate receiving component 13 to automatically extend to connect with the external reagent plate, or retract into the housing 1 for subsequent processing. Inside the hollow frame 131, there are mirror-symmetrically arranged clamping plates 133 for clamping and fixing the animal cell reagent plate 14 from both sides. The clamping plates 133 are not rigidly fixed, but are movably installed in the hollow frame 131 through the spacing adjustment component 134, so that they can adaptively adjust the clamping spacing according to the actual width of the animal cell reagent plate 14. The inner surface of the clamping plate 133 is covered with a rubber anti-slip layer 1331. This rubber anti-slip layer 1331 enhances the friction of the animal cell reagent plate 14 on the one hand, preventing it from slipping during the transfer process, and on the other hand, it plays a buffering and protective role, avoiding damage to the surface of the reagent plate or its internal sample during the clamping process, thus providing a reliable prerequisite for the subsequent automated processing.

[0098] Specifically, the spacing adjustment component 134 includes:

[0099] The bidirectional lead screw 1341 is horizontally installed in the hollow frame 131 via the bearing seat 1342;

[0100] The second slider 1343 has a top vertically fixed clamping plate 133, which is slidably installed in the second slide groove 1344 at the top of the hollow frame 131 and is threadedly connected to the bidirectional lead screw 1341.

[0101] The second servo motor 1345 is fixed to one end of the second slide groove 1344, and its output end drives the bidirectional lead screw 1341 to rotate through the bevel gear set 1346.

[0102] The spacing adjustment component 134 is the core mechanism in the reagent plate receiving component 13 for automatically adjusting the spacing of the clamping plates 133. Its structural design ensures adaptive clamping capability for animal cell reagent plates 14 of different sizes. The bidirectional lead screw 1341, as the core transmission component, is horizontally arranged inside the hollow frame 131 and is supported and positioned by bearing seats 1342 at both ends. The bearing seats 1342 are fixedly installed on the inner wall of the hollow frame 131, so that the bidirectional lead screw 1341 can rotate stably without axial movement or radial displacement. There are two second sliders 1343, corresponding to the clamping plates 133 on both sides respectively. The top of each second slider 1343 is vertically fixedly connected to a clamping plate 133, and its body is slidably installed in the second slide groove 1344 provided at the top of the hollow frame 131, thereby restricting its movement trajectory to only the horizontal direction. At the same time, the two second sliders 1343 are respectively connected to the bidirectional lead screw 1341. The two screws 1343 form a threaded engagement with opposite threaded sections. When the bidirectional screw 1341 rotates, the two second sliders 1343 move synchronously towards or away from each other due to the opposite thread rotation direction. This drives the clamping plates 133 on both sides to reduce or expand the distance between them. The second servo motor 1345 is fixedly installed at one end of the second slide groove 1344. Its output end is not directly connected to the bidirectional screw 1341, but is transmitted through a bevel gear set 1346. The bevel gear set 1346 consists of a pair of meshing bevel gears. It converts the rotational motion direction output by the second servo motor 1345 from the axial direction to the direction coaxial with the bidirectional screw 1341, thereby driving the bidirectional screw 1341 to rotate smoothly. This transmission method not only saves space, but also ensures transmission accuracy and running stability. It is compatible with animal cell reagent plates 14 of different widths and, together with the rubber anti-slip layer 1331, achieves reliable clamping, providing a stable positioning basis for subsequent automated processing.

[0103] Specifically, the transfer conveyor 7 includes:

[0104] The transfer conveyor 72 is rotatably fitted onto the outer wall of the central positioning column 5 via the first circumferential adjustment component 71;

[0105] The detection element 11 includes:

[0106] The telescopic base 111 is rotatably fitted onto the outer wall of the central positioning column 5 via the second circumferential adjustment component 112, and the front end of the base is fixedly installed with the conveying separation component 12.

[0107] Z-axis slide rail 1111 is vertically mounted on the side of telescopic base 111 via fixed bent rod 1112;

[0108] Wavelength transmitter 113 is slidably mounted on Z-axis slide rail 1111;

[0109] The transfer conveyor 7 and the detection component 11 are key actuators within the animal cell immunoassay analyzer, enabling reagent plate carrier transport and optical detection. Both are arranged around the central positioning post 5 and achieve precise spatial motion control through their respective adjustment components. The transfer conveyor 7 is mainly used to transfer the moving inner core 98 between the reagent plate receiver 13 and the high-throughput constant temperature incubation zone 8. The transfer conveyor 7 includes a transfer conveyor body 72 and a first circumferential adjustment component 71. The transfer conveyor body 72 is entirely fitted onto the outer wall of the central positioning post 5 and achieves circumferential adjustment through the first circumferential adjustment component 71. The rotation of the central positioning column 5 allows the transfer conveyor 72 to rotate to different angles in the horizontal plane, thereby aligning it with the reagent plate interface 3 or each incubation unit to complete the extraction or placement of the moving inner core 98. The first circumferential adjustment component 71 serves as a drive and guide mechanism, ensuring smooth rotation and accurate positioning of the transfer conveyor 72, providing fundamental support for high-throughput continuous processing. The detection component 11 performs optical detection and auxiliary unloading functions, and its main structure includes a telescopic base 111, a second circumferential adjustment component 112, a Z-axis slide rail 1111, and a fixed bent rod 1112. The telescopic base 111, along with the wavelength emitter 113, is also fitted onto the outer wall of the central positioning post 5. It rotates around the central positioning post 5 via the second circumferential adjustment assembly 112, allowing it to rotate to any position corresponding to an incubation unit or the delivery / separation component 12. The telescopic base 111 can perform radial telescopic movement. A delivery / separation component 12 is fixedly installed at the front end of the telescopic base 111 for performing the extraction of the moving inner core 98 and the ejection of the reagent plate. A Z-axis slide rail 1111 is vertically arranged on the side of the telescopic base 111 via a fixed bent rod 1112. This Z-axis slide rail 1111 extends along... The vertical extension provides a track for the wavelength emitter 113 to move up and down. The wavelength emitter 113 is slidably mounted on the Z-axis slide rail 1111 and can be adjusted in the vertical direction to perform optical density scanning on reagent plate wells at different heights. Through the rotational cooperation between the Z-axis slide rail 1111 and the telescopic base 111, the wavelength emitter 113 can cover all detection points in the entire incubation area, realizing comprehensive and real-time optical monitoring and completing dynamic optical detection of the cell reaction status during incubation. The two work together to ensure the full automation of the instrument process and the reliability of the detection data.

[0110] Specifically, the second circumferential adjustment component 112 has the same structure as the first circumferential adjustment component 71, including:

[0111] A hollow retaining ring 1121 is fixedly sleeved on the outer wall of the central positioning post 5, and the outer wall is provided with an annular toothed rack 1122;

[0112] The third servo motor 1123 is fixed inside the telescopic base 111, and the output gear 1124 meshes with the ring rack 1122.

[0113] The guide rod 1125 has one end fixed to the inner side of the telescopic base 111, and the other end is provided with a positioning ball 1126, which is movably installed in the annular ball groove 1127 on the outer wall of the hollow fixing ring 1121.

[0114] The second circumferential adjustment assembly 112 adopts the same structural design as the first circumferential adjustment assembly 71, and is used to achieve precise rotational movement around the central positioning post 5. Its core function is to provide stable and controllable circumferential positioning capability for the connected components. This structure includes a hollow fixing ring 1121, a third servo motor 1123, an output gear 1124, a guide rod 1125, a positioning ball 1126, and an annular ball groove 1127. The hollow fixing ring 1121 is fixedly sleeved on the outer wall of the central positioning post 5, serving as a stationary reference component for the entire adjustment assembly. Its outer wall is provided with an annular rack 1122. The ring rack 1122 is continuously distributed along the circumference and is used to mesh with the drive mechanism to transmit rotational power. The third servo motor 1123 is fixedly installed on the inner side of the telescopic base 111, and an output end gear 1124 is installed on its output shaft. The output end gear 1124 meshes with the ring rack 1122 on the outer wall of the hollow fixed ring 1121. When the third servo motor 1123 runs, the meshing transmission between the output end gear 1124 and the ring rack 1122 drives the telescopic base 111 to rotate around the central positioning column 5, thereby realizing the angle adjustment of the detection piece 11 in the horizontal plane. To ensure smooth rotation without radial offset, a guide rod 1125 is provided inside the telescopic base 111. One end of the guide rod 1125 is firmly connected to the telescopic base 111, and the other end is provided with a positioning ball 1126. The positioning ball 1126 is embedded and movably installed in an annular groove 1127 provided on the outer wall of the hollow fixing ring 1121. The annular groove 1127 extends along the circumference of the hollow fixing ring 1121, forming a closed annular track. The positioning ball 1126 slides in this track, guiding and limiting the rotational movement of the telescopic base 111, effectively preventing it from rotating out of control. During the process, it may shake, tilt, or detach, while bearing part of the radial load, thus improving the rigidity and running accuracy of the overall structure. The second circumferential adjustment component 112 achieves active rotation by driving the gear and rack system through the third servo motor 1123. At the same time, it relies on the ball groove guide mechanism composed of the guide rod 1125 and the annular ball groove 1127 to ensure motion stability and concentricity. This structure is not only applicable to the telescopic base 111 of the detection component 11, but also to the first circumferential adjustment component 71 in the transfer and conveying component 7. It embodies the design concept of modularity and universality, which helps to improve the reliability and maintenance convenience of the equipment.

[0115] Specifically, the constant temperature incubator 9 includes:

[0116] A hollow constant temperature sleeve 91 is fixedly sleeved on the outer wall of the central positioning column 5, and multiple independent constant temperature incubation spaces 92 are formed in the circumferential direction inside.

[0117] Heating plate 93 and temperature sensor 94 are embedded in the inner wall of each constant temperature incubation space 93;

[0118] The retractable sealing plate 95 is movably installed at the edge of the opening of the constant temperature incubation space 93 via an electric telescopic cylinder 96;

[0119] The positioning bracket 97 is fixed in the middle of the constant temperature incubation space 93 by a radial fixing rod, and a third rack 971 is symmetrically provided inside;

[0120] The isothermal incubation unit 9 is the core functional module in the animal cell immunoassay analyzer that enables precise control of the cell reaction environment. Its structural design revolves around the central positioning column 5, ensuring a high degree of independence and synergy among the incubation units in terms of spatial layout, temperature control, and mechanical connection. The hollow isothermal sleeve 91, serving as the overall support and heat conduction carrier, is fixedly fitted onto the outer wall of the central positioning column 5. Its interior is divided into multiple isolated isothermal incubation spaces 92 along the circumferential direction. Each isothermal incubation space 92 constitutes an independent incubation unit, accommodating a movable inner core 98 and the animal cell reagent plate 14 it carries, thereby supporting multi-channel operation. To avoid cross-interference between samples, a heating plate 93 and a temperature sensor 94 are embedded in the inner wall of each constant-temperature incubation space 92. The heating plate 93 provides a stable heat source to maintain the constant temperature environment required for incubation, while the temperature sensor 94 monitors the actual temperature in the space in real time and feeds the data back to the control system, forming a closed-loop temperature control mechanism to ensure the accuracy and stability of the incubation conditions. To ensure airtightness and environmental isolation during the incubation process, a retractable sealing plate 95 is provided at the opening edge of each constant-temperature incubation space 93. This retractable sealing plate 95 is driven by an electric telescopic cylinder 96 and can extend to close the incubation space when needed. The incubation space has an opening, forming a sealed cavity to prevent external airflow or contaminants from entering. When reagent plates need to be loaded or unloaded, the electric telescopic cylinder 96 drives the telescopic sealing plate 95 to retract, opening the channel to allow the movable inner core 98 to enter and exit. In the center of the constant temperature incubation space 93, a positioning bracket 97 is securely installed via a radial fixing rod. This positioning bracket 97 is located in the central area of ​​the incubation unit and is used to achieve precise docking with the movable inner core 98. A third rack 971 is symmetrically arranged inside the rack 971. The third rack 971 is arranged vertically and can engage with the lifting drive gear on the movable inner core 98 or the conveying separation component 12 or the transfer conveyor 72. Corresponding rack and pinion structures are spliced ​​to form a continuous transmission path, thereby providing a power transmission interface for the lifting and lowering movement of the moving inner core 98 within the incubation unit. In summary, the constant temperature incubation component 9 constructs multiple independent incubation spaces through the hollow constant temperature sleeve 91, achieves precise temperature control by combining the heating plate 93 and the temperature sensor 94, ensures environmental sealing by utilizing the retractable sealing plate 95 and the electric telescopic cylinder 96, and completes mechanical positioning and power linkage through the positioning card connector 97 and its built-in third rack 971. The overall structure takes into account the three core functions of thermal management, sealing protection and automated docking, providing a stable and reliable incubation basis for animal cell immune activity analysis.

[0121] Specifically, the conveying separator 12 and the transfer conveyor 72 have the same structure, both including:

[0122] The C-shaped outer frame 721 has vertical lifting grooves 722 and a first rack 723 on its inner walls on both sides;

[0123] The lifting inner frame 724 has T-shaped sliders 725 on both sides, which are slidably engaged in the lifting slide groove 722, and the second rack 726 is symmetrically fixed on the inner wall.

[0124] The lifting motor 727 is fixed to the side wall of the T-shaped slider 725, and the output end is provided with a first gear 728 that meshes with a first rack 723.

[0125] The lifting inner frame 724 has a docking protrusion 729 at one end, which docks with the positioning groove 972 of the positioning card seat 97.

[0126] The second rack 726 and the third rack 971 are joined together to form a continuous rack belt;

[0127] The conveying separator 12 and the transfer conveyor 72 adopt the same mechanical structure design. This structure aims to achieve precise vertical lifting and lowering movement and complete reliable docking with the positioning card holder 97 in the constant temperature incubation area, thereby ensuring the smooth transfer of the moving inner core 98 between different functional areas. The C-shaped outer frame 721 serves as the overall support structure, with an open ring shape, which facilitates arrangement around the central positioning column 5 and leaves operating space. Vertical lifting grooves 722 and first racks 723 are respectively provided on its two inner walls. The vertical lifting grooves 722 are used to guide the vertical movement of the internal components, while the first rack 723 is fixed vertically to the inner wall of the C-shaped outer frame 721 as a transmission element for lifting drive. The lifting inner frame 724 is set on the C-shaped outer frame 721. Inside the inner frame 724 (21), T-shaped sliders 725 are provided on both sides. The shape of the T-shaped sliders 725 matches the vertical lifting groove 722, allowing them to slide and engage, thus restricting the inner lifting frame 724 to move only in the vertical direction and preventing it from deflecting or swaying during lifting. On the inner wall of the inner lifting frame 724, second racks 726 are symmetrically fixed. These racks 726 also extend vertically and mesh with the drive mechanism of the moving inner core 98. The lifting motor 727 is fixedly installed on the side wall of the T-shaped sliders 725, and its output end is equipped with a first gear 728. This first gear 728 meshes with the first rack 723 on the inner wall of the C-shaped outer frame 721. When the lifting motor 727 operates, the first gear 728 meshes with the first rack 723 on the inner wall of the C-shaped outer frame 721. The meshing transmission of the 3rd gear drives the entire lifting inner frame 724 to rise or fall relative to the C-shaped outer frame 721, thereby driving the movable inner core 98 carried or docked inside to complete the movement of entering and exiting the incubation unit. At one end of the lifting inner frame 724, there is a docking protrusion 729. The geometry of this docking protrusion 729 matches the positioning groove 972 opened on the positioning card holder 97, enabling precise mechanical alignment during transport or separation, ensuring a stable connection between the movable inner core 98 and the incubation unit. Furthermore, after the lifting inner frame 724 and the positioning card holder 97 are docked, the second rack 726 fixed to its inner wall will tightly engage with the third rack 971 symmetrically arranged inside the positioning card holder 97 in the vertical direction, together forming a... A continuous rack belt provides a complete meshing path for the lifting drive gear on the moving inner core 98, enabling it to independently complete lifting and lowering actions within the incubation unit without continuous intervention from external mechanisms. In summary, the conveying separator 12 and the transfer conveyor 72 achieve high-precision vertical lifting and lowering functions through the nested sliding structure of the C-shaped outer frame 721 and the lifting inner frame 724, combined with the gear and rack transmission system. The cooperation between the docking protrusion 729 and the positioning groove 972 ensures accurate mechanical alignment, and the splicing of the second rack 726 and the third rack 971 forms a continuous transmission interface, providing the necessary conditions for the autonomous movement of the moving inner core 98 within the incubation unit. The overall structure takes into account stability, precision, and automated collaborative capabilities.

[0128] Specifically, the movable inner core 98 includes:

[0129] The connector frame 981 has a reagent plate slot 982 at the front end;

[0130] The lifting assembly 983 is mirror-symmetrically disposed on the side wall of the plug-in frame 981;

[0131] The push assembly 984 includes an electric push rod 9841 and a push plate 9842. The electric push rod 9841 is fixed to the inner wall of the plug frame 981, and the push plate 9842 slides against the bottom surface of the reagent plate slot 982.

[0132] The movable core 98 is the core functional unit of the animal cell immunoassay analyzer, used for carrying, positioning, and transporting the animal cell reagent plate 14. Its structural design integrates three major functions: carrying, lifting drive, and automatic unloading, ensuring stable and reliable operation of the reagent plate during incubation, detection, and separation. The movable core 98 specifically includes a connector frame 981, a lifting assembly 983, and a pushing assembly 984. The connector frame 981 forms the main frame of the movable core 98, with a robust yet lightweight overall structure. Its front end has a reagent plate slot 982, which is used to accommodate and restrain the animal cells. The cell reagent plate 14 is designed to maintain a fixed posture during movement and incubation, preventing displacement or detachment and providing a stable sample position reference for subsequent optical detection. The lifting assembly 983 is mirror-symmetrically arranged on both sides of the insertion frame 981, driving the entire moving inner core 98 to move vertically. This symmetrical layout ensures balanced force during lifting, avoiding tilting or jamming caused by unilateral drive, thus guaranteeing the stability and positioning accuracy of the moving inner core 98 when entering and exiting the constant temperature incubation space 93. The lifting assembly 983 connects with an external continuous rack belt (composed of a second rack). 726 and the third rack 971 are spliced ​​together to form a meshing mechanism, realizing power input and vertical displacement control. The push assembly 984 is integrated inside the insertion frame 981 and is used to automatically push the completed animal cell reagent plate 14 from the reagent plate slot 982 at the end of the analysis process. The push assembly 984 includes an electric push rod 9841 and a push plate 9842. The electric push rod 9841 is fixedly installed on the inner wall of the insertion frame 981 as a linear drive source. The push plate 9842 is connected to the output end of the electric push rod 9841 and slides against the bottom surface of the reagent plate slot 982. When the electric push rod 9841 extends, the push plate 9842 moves forward along the bottom surface of the reagent plate slot 982, pushing the reagent plate 14 horizontally to the direction of the discharge port 15, completing the automatic unloading operation. The moving inner core 98 provides a stable support platform for the reagent plate through the plug-in frame 981. It achieves linkage lifting with the conveying and incubation mechanism by relying on the lifting component 983, and completes the automatic unloading of the reagent plate with the help of the pushing component 984. The three work together to make the moving inner core 98 the only carrier throughout the entire process of sample introduction, incubation, detection and unloading, effectively supporting the high throughput and fully automated operation capability of the instrument.

[0133] Specifically, the lifting assembly 983 includes:

[0134] The fourth servo motor 9831 is fixed in the embedded groove on the side wall of the plug frame 981;

[0135] The third gear 9832 is fixed to the output end of the fourth servo motor 9831;

[0136] The third gear 9832 is meshed with the continuous rack belt;

[0137] The analytical method of the animal cell immunomodulator includes the following steps:

[0138] a. Set the incubation temperature, optical density threshold, and activity calculation parameters on the display screen 2;

[0139] b. The reagent plate receiving component 13 receives the animal cell reagent plate 14, and the clamping plate 133 fixes the animal cell reagent plate 14.

[0140] c. Transfer conveyor 7 extracts the target moving inner core 98 to the transfer conveyor area 6;

[0141] d. The reagent plate receiving component 13 horizontally inserts the animal cell reagent plate 14 into the reagent plate slot 982 of the movable inner core 98;

[0142] e. The transfer conveyor 7 returns the movable inner core 98, which is loaded with animal cell reagent plate 14, to the original incubation unit;

[0143] f. The constant temperature incubator 9 is incubated at the set temperature, and the detection device 11 monitors the optical density in real time.

[0144] g. When the rate of change of optical density is continuously lower than the set threshold, the detection element 11 determines that the incubation is complete;

[0145] h. Display operation screen 2 calculates and displays the cell immune activity index;

[0146] i. The conveying and separating component 12 extracts the moving inner core 98 and pushes the component 984 to push out the animal cell reagent plate 14;

[0147] j. The animal cell reagent plate 14 is discharged through the reagent plate interface 3, and the moving inner core 98 is reset.

[0148] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0149] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An animal cell immune activity analyzer, characterized in that, include: The housing (1) has a display and operation screen (2) at its upper end and a reagent plate interface (3) at its front end. The reagent plate interface (3) has a retractable reagent plate receiver (13) for receiving animal cell reagent plates (14). The side wall has an outlet (15) and a corresponding collection box (16). A coaxial integrated bias analysis area (4) is located inside the housing (1), with a vertically extending central positioning post (5) at its center. The components are integrated from top to bottom with the central positioning post (5) as the axis. Transfer conveying area (6): It is provided with a transfer conveying component (7) that can rotate and rise around the central positioning column (5). The transfer conveying component (7) includes a first circumferential adjustment component (71) and a transfer conveying body (72) that is rotatably fitted on the outer wall of the central positioning column (5) through the first circumferential adjustment component (71). High-throughput constant temperature incubation area (8): It is equipped with a constant temperature incubation component (9) that is fixedly sleeved on the outer wall of the central positioning column (5). Multiple independent incubation units are evenly distributed in the circumferential direction inside. Each incubation unit is equipped with a movable inner core (98) that can be raised and lowered. Detection separation area (10): It is provided with a detection element (11) that can rotate around the central positioning column (5) and a conveying separation element (12). The detection element (11) is fixed on one side of the circumferential direction of the conveying separation element (12). Each component is linked by a moving inner core (98) to achieve high-throughput continuous processing. The moving inner core (98) corresponds one-to-one with the incubation unit and the carrier is transferred only through the transfer conveyor (7) and the conveyor separation unit (12).

2. The animal cell immune activity analyzer according to claim 1, characterized in that: The reagent plate receiver (13) includes: The hollow frame (131) can be horizontally telescopically installed in the reagent plate interface (3) via the moving component (132), and its side wall is provided with a first slider (1311) which is slidably connected to the first groove (1312) on the side wall of the reagent plate interface (3). The moving component (132) includes: The first servo motor (1321) is fixed to the side wall of the reagent plate interface (3); The first lead screw (1322) is coaxially connected to the output end of the first servo motor (1321), and the thread passes through the screw hole on the side wall of the hollow frame (131); A mirror-symmetrical clamping plate (133) is movably mounted in the hollow frame (131) via a spacing adjustment component (134), and a rubber anti-slip layer (1331) is provided on the inner side of the clamping plate (133).

3. The animal cell immune activity analyzer according to claim 2, characterized in that: The spacing adjustment component (134) includes: The bidirectional lead screw (1341) is horizontally installed inside the hollow frame (131) via a bearing seat (1342); The second slider (1343) is vertically fixed to the top clamping plate (133), and is slidably installed in the second slide groove (1344) at the top of the hollow frame (131), and is threadedly connected to the bidirectional lead screw (1341). The second servo motor (1345) is fixed at one end of the second slide groove (1344), and its output end drives the bidirectional lead screw (1341) to rotate through the bevel gear set (1346).

4. The animal cell immune activity analyzer according to claim 1, characterized in that: The transfer transport component (7) includes: The transfer conveyor (72) is rotatably fitted onto the outer wall of the central positioning column (5) via the first circumferential adjustment component (71); The detection element (11) includes: The telescopic base (111) is rotatably fitted onto the outer wall of the central positioning column (5) via the second circumferential adjustment component (112), and the front end of the base is fixedly installed with the conveying separation component (12). The Z-axis slide rail (1111) is vertically mounted on the side of the telescopic base (111) via a fixed bent rod (1112); Wavelength transmitter (113) is slidably mounted on Z-axis slide rail (1111).

5. The animal cell immune activity analyzer according to claim 4, characterized in that: The second circumferential adjustment component (112) has the same structure as the first circumferential adjustment component (71), including: A hollow fixing ring (1121) is fixedly sleeved on the outer wall of the central positioning post (5), and the outer wall is provided with an annular toothed rack (1122). The third servo motor (1123) is fixed inside the telescopic base (111), and the output end gear (1124) meshes with the ring rack (1122). The guide rod (1125) is fixed at one end to the inside of the telescopic base (111), and the other end is provided with a positioning ball (1126), which is movably installed in the annular ball groove (1127) on the outer wall of the hollow fixing ring (1121).

6. The animal cell immunomodulator according to claim 1, characterized in that: The constant temperature incubator (9) includes: Hollow constant temperature sleeve (91) is fixedly sleeved on the outer wall of the central positioning column (5), and multiple independent constant temperature incubation spaces (92) are formed in the circumferential direction inside. Heating plates (93) and temperature sensors (94) are embedded in the inner walls of each constant temperature incubation space (93); The retractable sealing plate (95) is movably installed at the edge of the opening of the constant temperature incubation space (93) via an electric telescopic cylinder (96); The positioning card holder (97) is fixed in the middle of the constant temperature incubation space (93) by a radial fixing rod, and a third rack (971) is symmetrically provided inside.

7. The animal cell immunomodulator according to claim 6, characterized in that: The conveying separation component (12) and the transfer conveyor (72) have the same structure, both including: The C-shaped outer frame (721) has vertical lifting grooves (722) and a first rack (723) on its inner walls on both sides. The lifting inner frame (724) has T-shaped sliders (725) on both sides, which are slidably engaged in the lifting slide groove (722), and the inner wall is symmetrically fixed with the second rack (726). The lifting motor (727) is fixed to the side wall of the T-shaped slider (725), and the output end is provided with a first gear (728) that meshes with the first rack (723). The lifting inner frame (724) has a docking protrusion (729) at one end, which docks with the positioning groove (972) of the positioning card seat (97); The second rack (726) and the third rack (971) are spliced ​​together to form a continuous rack belt.

8. The animal cell immune activity analyzer according to claim 1, characterized in that: The movable inner core (98) includes: The connector frame (981) has a reagent plate slot (982) at the front end. The lifting assembly (983) is mirror-symmetrically disposed on the side wall of the plug frame (981); The pushing assembly (984) includes an electric push rod (9841) and a push plate (9842). The electric push rod (9841) is fixed to the inner wall of the plug frame (981), and the push plate (9842) slides against the bottom surface of the reagent plate slot (982).

9. The animal cell immune activity analyzer according to claim 8, characterized in that: The lifting assembly (983) includes: The fourth servo motor (9831) is fixed in the embedded groove on the side wall of the plug frame (981); The third gear (9832) is fixed to the output end of the fourth servo motor (9831); The third gear (9832) is meshed with the continuous rack belt.

10. The analytical method of the animal cell immunomodulator according to any one of claims 1-9, characterized in that, Includes the following steps: a. Set the incubation temperature, optical density threshold and activity calculation parameters on the display operation screen (2); b. The reagent plate receiving component (13) receives the animal cell reagent plate (14), and the clamping plate (133) fixes the animal cell reagent plate (14). c. The transfer conveyor (7) extracts the target moving inner core (98) to the transfer conveyor area (6); d. The reagent plate receiving component (13) horizontally inserts the animal cell reagent plate (14) into the reagent plate slot (982) of the movable inner core (98). e. The transfer conveyor (7) returns the movable inner core (98) containing the animal cell reagent plate (14) back to the original incubation unit; f. The constant temperature incubator (9) is incubated at the set temperature, and the detection unit (11) monitors the light density in real time. g. When the rate of change of optical density is continuously lower than the set threshold, the detection element (11) determines that the incubation is complete; h. Display operation screen (2) calculates and displays the cell immune activity index; i. The conveying and separating component (12) extracts the moving inner core (98) and the pushing component (984) pushes out the animal cell reagent plate (14). j. The animal cell reagent plate (14) is discharged through the reagent plate interface (3), and the moving inner core (98) is reset.

Images