Health-preserving tea capable of tonifying qi and blood and preparation method of health-preserving tea

Abstract

The present invention discloses qi and blood tonifying health preserving tea and a preparation method thereof, and particularly relates to the technical field of health care products, the health preserving tea is prepared from a fermented mulberry extract, a curcumin-phospholipid compound, oligopeptide chelated iron, a cape jasmine extract, microencapsulated lotus plumule alkaloid, natural sugar substitutes and a flavor regulator according to a specific weight ratio. The preparation method comprises the following steps: respectively preparing the fermented mulberry extract and the microencapsulated lotus plumule alkaloid, mixing the fermented mulberry extract and the microencapsulated lotus plumule alkaloid with other components, granulating, drying at low temperature and packaging. Through cooperation of multiple components and a specific process, the traditional efficacy of tonifying qi and blood is remarkably improved, the mitochondrial function can be improved from the level of cell energy metabolism, and meanwhile, the composition has the advantages of being stable in component, good in taste and convenient to take.

Description

Technical Field

[0001] This invention relates to the field of health product technology, and more specifically, to a health-preserving tea for replenishing qi and blood and its preparation method. Background Technology

[0002] Qi- and blood-tonifying health teas have always been an important part of traditional Chinese medicine's dietary therapy and health preservation. Their traditional formulas mostly rely on simple combinations of medicinal and edible ingredients such as red dates, goji berries, longan, and astragalus. Although these products have a certain health-preserving foundation, they generally have the following technical limitations: First, the formulas are mostly based on historical experience, with similar components and proportions. Functional claims often remain at the level of traditional macro concepts such as replenishing qi and blood and calming the mind, lacking specific explanations of the mechanisms of action related to modern physiology or cell biology. This leads to insufficient product innovation and serious homogenization. Second, the preparation process is usually quite crude, mostly involving only the crushing and physical mixing of raw materials. The dissolution efficiency of active ingredients is low, the stability is poor, and they are easily deactivated by environmental factors during storage. In addition, the inherent taste of some raw materials also affects the palatability of the product and the user's long-term adherence to use.

[0003] While current market research and related studies are dedicated to improving the efficacy and experience of such products, most improvements are still limited to increasing or decreasing the types of raw materials or applying conventional extraction processes, failing to fundamentally solve systemic technical problems such as low bioavailability of active ingredients, unclear mechanisms of efficacy, and poor overall stability.

[0004] Therefore, in view of the shortcomings of the prior art, this application provides a health-preserving tea for replenishing qi and blood and its preparation method. Summary of the Invention

[0005] In order to overcome the above-mentioned defects of the prior art, the present invention provides a health-preserving tea for replenishing qi and blood and its preparation method, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a health-preserving tea for replenishing qi and blood, made from raw materials comprising the following parts by weight: Fermented mulberry extract 8-12 parts, curcumin-phospholipid complex 1-3 parts, oligopeptide chelated iron 2-4 parts, gardenia extract 1-2 parts, microencapsulated lotus seed core alkaloids 0.3-0.8 parts, and natural sugar substitutes and flavor modifiers 3-8 parts.

[0007] Preferably, the mixture contains 10 parts fermented mulberry extract, 2 parts curcumin-phospholipid complex, 3 parts oligopeptide chelated iron, 1.5 parts gardenia extract, 0.5 parts microencapsulated lotus seed core alkaloids, and 5-6 parts natural sugar substitutes and flavor modifiers.

[0008] Preferably, the natural sugar substitute and flavor modifier includes at least one of fructooligosaccharides, erythritol, and yeast extract.

[0009] Preferably, the microencapsulated lotus seed core alkaloid is an inclusion complex formed by encapsulating an ethanol extract of lotus seed core using β-cyclodextrin encapsulation technology.

[0010] Preferably, the curcumin-phospholipid complex is a complex formed by curcumin and soybean phospholipids or egg yolk phospholipids, and the oligopeptide chelated iron is a chelate of corn oligopeptide or soybean oligopeptide and iron ions.

[0011] This invention also provides a preparation method for preparing the above-mentioned qi-tonifying and blood-nourishing tea, specifically including the following steps: S1. Prepare fermented mulberry extract and microencapsulated lotus seed core alkaloids respectively; S2. Fermented mulberry extract, curcumin-phospholipid complex, oligopeptide chelated iron, gardenia extract and microencapsulated lotus seed core alkaloids are premixed in proportion to obtain the core efficacy mixture. S3. Mix the core efficacy mixture obtained in step S2 with natural sugar substitutes and flavor modifiers, and then granulate it. S4. The obtained granules are dried and packaged to obtain a compound health tea product.

[0012] Preferably, in step S1, the preparation method of the fermented mulberry extract includes: anaerobic fermentation of mulberries with a mixed strain of Lactobacillus plantarum and Lactobacillus acidophilus, followed by low-temperature ultrasonic-assisted water extraction after fermentation, and then membrane filtration, concentration, and spray drying of the extract.

[0013] Preferably, in step S1, the preparation method of the microencapsulated lotus seed core alkaloid includes: extracting lotus seed core with ethanol to obtain an extract rich in methyl lotus seed core alkaloid, then performing an inclusion reaction with a β-cyclodextrin solution, and spray-drying the reaction solution to obtain the microencapsulated product.

[0014] Preferably, in step S3, the granulation is wet granulation using pure water as a binder, and in step S4, the drying is fluidized bed drying at a temperature of 40-45℃, and the moisture content of the dried product is ≤5%.

[0015] The technical effects and advantages of this invention are as follows: 1. This invention scientifically combines fermented mulberry extract, curcumin-phospholipid complex, oligopeptide chelated iron, gardenia extract and microencapsulated lotus seed core alkaloids. Based on the multi-target synergistic mechanism of anti-oxidation, improvement of cellular energy metabolism and regulation of energy sensing pathway, it can significantly improve the hemoglobin content, red blood cell count and exercise endurance of qi and blood deficiency model animals, achieving a substantial breakthrough in the efficacy of replenishing qi and blood. 2. The product of this invention can effectively improve the energy level of cells damaged by oxidative stress, stabilize mitochondrial function and efficiently remove reactive oxygen species, thereby improving the energy metabolism efficiency of cells, especially mitochondria, and giving the traditional macroscopic effects of replenishing qi and blood a clear mechanistic basis at the level of modern cell biology, realizing a systemic effect from symptom improvement to cell function recovery. 3. The probiotic fermentation, microencapsulation, phospholipid complexation and peptide chelation processes used in this invention significantly improve the bioavailability, stability and taste compliance of the core active ingredients. Combined with subsequent low-temperature granulation and nitrogen-filled packaging processes, the physical stability and chemical activity of the product are further guaranteed, giving it a better shelf life and user experience. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the present invention. Detailed Implementation

[0017] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

[0018] Example 1 This invention provides a health-preserving tea for replenishing qi and blood, made from the following ingredients in parts by weight: 10 parts fermented mulberry extract, 2 parts curcumin-phospholipid complex, 3 parts oligopeptide chelated iron, 1.5 parts gardenia extract (commercially available, total iridoid glycoside content ≥30%), 0.5 parts microencapsulated lotus seed core alkaloids, 4.5 parts oligofructose, and 0.5 parts yeast extract.

[0019] This invention also provides a preparation method for preparing the above-mentioned qi-tonifying and blood-nourishing tea, specifically including the following steps: S1. Prepare fermented mulberry extract and microencapsulated lotus seed core alkaloids separately, specifically: Preparation of fermented mulberry extract: Dried mulberries were pulverized and mixed with water at a ratio of 1:8. After sterilization, the mixture was inoculated with 2% of a mixed strain of Lactobacillus plantarum and Lactobacillus acidophilus (1:1) and anaerobic fermented at 37℃ for 72 hours. The fermentation broth was then subjected to ultrasonic-assisted extraction at 50℃ and 300W power for 1 hour. The extract was coarsely filtered through a 100-mesh sieve and then finely filtered through a 0.45μm ceramic membrane. The filtrate was concentrated to a soluble solids content of about 30% and then spray-dried (inlet air temperature 160℃, outlet air temperature 85℃) to obtain a brownish-red powder. Preparation of microencapsulated lotus seed core alkaloids: Lotus seed cores were extracted twice with 70% ethanol solution under reflux at 60℃ for 1.5 h each time. The extracts were combined and concentrated under reduced pressure to obtain an extract. The extract and β-cyclodextrin (at a mass ratio of core material: wall material = 1:8) were dissolved together in an appropriate amount of warm water and stirred for 4 h. The encapsulation solution was spray-dried (inlet air temperature 140℃, outlet air temperature 75℃) to obtain a white to light yellow powder. S2. Fermented mulberry extract, curcumin-phospholipid complex, oligopeptide chelated iron, gardenia extract and microencapsulated lotus seed core alkaloids are mixed at low speed for 30 minutes in a three-dimensional motion mixer in proportion to obtain the core efficacy mixture. The curcumin-phospholipid complex was obtained by dissolving curcumin and soybean phospholipid together in an appropriate amount of ethanol at a mass ratio of 1:2, stirring and reacting at 60°C for 3 hours, then recovering the ethanol under reduced pressure, drying under vacuum, and pulverizing through an 80-mesh sieve. Oligopeptide chelated iron is prepared by dissolving corn oligopeptides in water, adjusting the pH to 7.0 with NaOH, slowly adding ferrous sulfate heptahydrate solution (at a peptide:iron ion molar ratio of 3:1), stirring and chelating at 50°C for 2 hours, and then precipitating the reaction solution with ethanol, centrifuging, washing, and vacuum drying to obtain a light yellow powder. S3. Add the core functional mixture obtained in step S2, oligofructose, and yeast extract into a wet mixing granulator and mix for 5 minutes. Slowly spray pure water (about 15% of the total weight of dry materials) as a binder to make a soft material. S4. Granulate the soft material through a 20-mesh sieve using a gyratory granulator. Place the wet granules in a fluidized bed dryer and dry them at 42°C until the moisture content is 4.8%. After the dried granules are cooled to room temperature, immediately package them in nitrogen-filled aluminum foil bags, 2.5 grams per bag, to obtain the health tea product.

[0020] Example 2 The difference between Example 2 and Example 1 is that: 8 parts fermented mulberry extract, 3 parts curcumin-phospholipid complex, 2 parts oligopeptide chelated iron, 2 parts gardenia extract, 0.8 parts microencapsulated lotus seed core alkaloids, 7 parts erythritol, and 1 part yeast extract.

[0021] The preparation process is the same as in Example 1.

[0022] Example 3 The difference between Example 3 and Example 1 is that: 12 parts fermented mulberry extract, 1 part curcumin-phospholipid complex, 4 parts oligopeptide chelated iron, 1 part gardenia extract, 0.3 parts microencapsulated lotus seed core alkaloids, 5 parts oligofructose, 2 parts erythritol, and 1 part yeast extract.

[0023] The preparation process is the same as in Example 1.

[0024] Comparative Example 1 Comparative Example 1 uses the following ingredient ratios: 10 parts ordinary mulberry powder, 2 parts ordinary curcumin powder, 3 parts ferrous fumarate (providing an equal amount of iron), 1.5 parts gardenia powder, 0.5 parts ordinary lotus seed core powder, and 5 parts white sugar.

[0025] The preparation method is as follows: all the above-mentioned powdered raw materials are directly put into a three-dimensional motion mixer and mixed for 30 minutes to obtain the product.

[0026] Comparative Example 2 Comparative Example 2 follows the same formulation as Example 1, but without the addition of curcumin-phospholipid complex and oligopeptide chelated iron; the missing weight is made up with an equal amount of oligofructose.

[0027] The preparation process is the same as in Example 1.

[0028] Comparative Example 3 Comparative Example 3 used the exact same types and weights of raw materials as Example 1, but eliminated all special processes. Specifically, it used ordinary mulberry powder, ordinary curcumin powder, ferrous sulfate heptahydrate, ordinary gardenia powder, and ordinary lotus seed core powder. After simply mixing all the raw materials, it was mechanically pulverized to pass through an 80-mesh sieve to obtain a powdered product.

[0029] Comparative Example 4 We selected commercially available blood-nourishing teas with red dates, goji berries, and longan as the main ingredients as a market comparison.

[0030] To verify the technical effect of the product of the present invention, the following tests were conducted on the health teas of the above embodiments and comparative examples: 1. In vitro cell experiments, used to evaluate improvements in cellular energy metabolism activity, are conducted as follows: Take the products from each example and comparative example, and prepare drug-containing serum mimics of different concentrations with cell culture medium (after standardized extraction processing). Using human umbilical vein endothelial cells, establish a hydrogen peroxide-induced oxidative stress injury model to simulate the decreased cell function under the state of qi and blood deficiency. Intracellular ATP content was measured using an ATP assay kit (fluorescence method) to reflect the cell's direct energy level. Mitochondrial membrane potential was detected using the JC-1 fluorescent probe to reflect mitochondrial functional status. The DCFH-DA fluorescent probe was used to detect the level of reactive oxygen species (ROS), reflecting the degree of cellular oxidative stress.

[0031] The test results are shown in the table below: (Based on the model group, the data are relative percentages, the higher the value, the better the improvement; "-" indicates not detected)

[0032] Table 1 shows the in vitro cell experiment data for the examples and comparative examples. 2. Animal model experiments were conducted to evaluate the traditional efficacy of tonifying qi and blood. The specific methods are as follows: SPF-grade SD rats, half male and half female, were used to establish a model of qi and blood deficiency by a combination of bloodletting and cyclophosphamide injection. The rats were divided into a normal group, a model group, each example group (administered by gavage at 10 times the human recommended dose), a control group 1, and a control group 4. The rats were administered the drugs for 14 consecutive days. Test metrics: Peripheral blood count: The fully automated blood cell analyzer detects hemoglobin (HGB) content and red blood cell count (RBC). Physical fitness test: Record the time spent swimming to exhaustion; Organ index: Calculates the thymus and spleen indices to reflect the status of immune organs.

[0033] The test results are shown in the table below: (Data are mean ± standard deviation)

[0034] Table 2 shows the animal model experimental data for the examples and comparative examples. The data in the table above shows that: 1. This invention, through the combination of fermented mulberry extract (antioxidant protection), curcumin-phospholipid complex and oligopeptide chelated iron (synergistically improves energy metabolism), and gardenia and microencapsulated lotus seed core extract (regulates energy sensing pathways), shows significantly better effects than comparative examples 1, 2 and 3 in terms of cellular energy metabolism levels (ATP content, mitochondrial membrane potential) and traditional qi and blood replenishment indicators (HGB, RBC, physical strength), proving the inventiveness and synergistic nature of its formulation design; 2. In vitro experimental data confirm that the product of this invention can effectively improve the energy level of cells under oxidative stress damage, stabilize mitochondrial function, and efficiently remove ROS, surpassing the efficacy of traditional health teas. 3. Comparing Example 1 and Comparative Example 3, it can be seen that key processes such as fermentation, microencapsulation, and complex preparation significantly improve the bioactivity of the product, proving that the preparation method of the present invention has a good effect on the final efficacy of the product. 4. In animal experiments, the embodiments of the present invention were superior to the comparative example 4 in all aspects of the core indicators of replenishing qi and blood, such as increasing hemoglobin and restoring physical strength, demonstrating its stronger efficacy and market competitiveness.

[0035] In summary: The health-preserving tea and its preparation method provided by this invention, through the combination of creative formulation design and key processes, successfully integrates the traditional effects of replenishing qi and blood with the modern mechanism of improving cellular energy metabolism. The resulting product shows significant and outstanding technological progress in cell vitality and animal models, and has good market application prospects.

[0036] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A health-preserving tea for replenishing qi and blood, characterized in that: Made from the following raw materials in parts by weight: Fermented mulberry extract 8-12 parts, curcumin-phospholipid complex 1-3 parts, oligopeptide chelated iron 2-4 parts, gardenia extract 1-2 parts, microencapsulated lotus seed core alkaloids 0.3-0.8 parts, and natural sugar substitutes and flavor modifiers 3-8 parts.

2. The qi- and blood-tonifying health tea according to claim 1, characterized in that: The raw materials are in the following weight proportions: 10 parts fermented mulberry extract, 2 parts curcumin-phospholipid complex, 3 parts oligopeptide chelated iron, 1.5 parts gardenia extract, 0.5 parts microencapsulated lotus seed core alkaloids, and 5-6 parts natural sugar substitutes and flavor modifiers.

3. The qi- and blood-tonifying health-preserving tea according to claim 2, characterized in that: The natural sugar substitutes and flavor modifiers include at least one of fructooligosaccharides, erythritol, and yeast extract.

4. The qi- and blood-tonifying health tea according to claim 3, characterized in that: The microencapsulated lotus seed core alkaloids are inclusion complexes formed by encapsulating lotus seed core ethanol extract using β-cyclodextrin encapsulation technology.

5. The qi- and blood-tonifying health-preserving tea according to claim 4, characterized in that: The curcumin-phospholipid complex is a complex formed by curcumin and soybean phospholipids or egg yolk phospholipids, and the oligopeptide chelated iron is a chelate of corn oligopeptide or soybean oligopeptide and iron ions.

6. A preparation method for preparing the qi-tonifying and blood-nourishing tea according to claim 5, characterized in that: Specifically, the following steps are included: S1. Prepare fermented mulberry extract and microencapsulated lotus seed core alkaloids respectively; S2. Fermented mulberry extract, curcumin-phospholipid complex, oligopeptide chelated iron, gardenia extract and microencapsulated lotus seed core alkaloids are premixed in proportion to obtain the core efficacy mixture. S3. Mix the core efficacy mixture obtained in step S2 with natural sugar substitutes and flavor modifiers, and then granulate it. S4. The obtained granules are dried and packaged to obtain a compound health tea product.

7. The preparation method according to claim 6, characterized in that: In step S1, the preparation method of the fermented mulberry extract includes: anaerobic fermentation of mulberries with a mixed strain of Lactobacillus plantarum and Lactobacillus acidophilus, followed by low-temperature ultrasonic-assisted water extraction after fermentation, and then membrane filtration, concentration and spray drying of the extract.

8. The preparation method according to claim 7, characterized in that: In step S1, the preparation method of the microencapsulated lotus seed core alkaloid includes: extracting lotus seed core with ethanol to obtain an extract rich in methyl lotus seed core alkaloid, then performing an inclusion reaction with a β-cyclodextrin solution, and spray drying the reaction solution to obtain the microencapsulated product.

9. The preparation method according to claim 8, characterized in that: In step S3, the granulation is wet granulation, using purified water as a binder; In step S4, the drying is carried out by fluidized bed drying at a temperature of 40-45℃, and the moisture content of the dried product is ≤5%.

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