A protein gel extraction unit and an electrophoresis extraction device comprising the same

Abstract

This invention relates to the field of protein extraction technology, specifically to a protein gel extraction unit and an electrophoretic extraction device comprising it. The protein gel extraction unit includes a tray body with a closed annular electrophoresis tank on its upper surface. Before electrophoresis, the protein gel is cut into multiple strips, and the length of the electrophoresis tank allows several strips to be coiled sequentially within the tank to form a closed annular structure. A first electrode and a second electrode, also closed annular structures, are respectively disposed on the two side walls of the electrophoresis tank, forming an electrophoretic electric field between the first and second electrodes. During use, the gel strips do not need to be chopped; they can be directly coiled within the electrophoresis tank. Proteins migrate out from one side of the strip, resulting in rapid aggregation, significantly improved electrophoresis efficiency, no need for liquid replacement, and high protein purity. The electrophoretic extraction device includes multiple extraction units connected in parallel. Connecting multiple extraction units in parallel by stacking them for electrophoresis not only saves space but also simplifies the circuit connection.

Description

Technical Field

[0001] This invention relates to the field of protein extraction technology, specifically to a protein gel extraction unit and an electrophoretic extraction apparatus comprising the same. Background Technology

[0002] Protein gel electrophoresis is a widely used separation and analysis technique in biochemistry, molecular biology, and proteomics. Its core principle is to separate different proteins by utilizing the differences in protein migration rates within a gel matrix under an electric field. Based on the molecular weight, charge, shape, and other characteristics of proteins, gel electrophoresis can be divided into several types, such as SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and native gel electrophoresis (Native-PAGE). SDS-PAGE is the most commonly used method, using sodium dodecyl sulfate (SDS) to denature proteins and uniformly assign them a negative charge, thus achieving molecular weight-based separation. However, SDS-PAGE only separates proteins into bands; the proteins remain trapped within the gel and require further electrophoretic extraction or soaking extraction to obtain a protein solution. Current electrophoretic extraction devices typically involve crushing the gel strips and placing them in a dialysis bag. The dialysis bag is placed in the middle of an electrophoresis tank, and electrophoresis buffer is added. Electrophoresis is then performed by applying current to both ends of the tank. After the proteins migrate out of the gel residue, the dialysis bag is opened, the residue is filtered out, and the protein solution is obtained. It has the following drawbacks: 1. The electric field distribution cross section between the positive and negative electrodes in the electrophoresis tank is large. The electrophoresis solution is a low-impedance path relative to the gel strip in the dialysis bag, which can cause a short circuit, causing the current to bypass the dialysis bag, resulting in a low current through the gel strip, a long electrophoresis time, and severe heating of the electrophoresis system; 2. Multiple gel strips require multiple dialysis bags, making the pre- and post-electrophoresis processing cumbersome; 3. The solution needs to be changed every two hours, requiring multiple changes in between. At the same time, in order to prevent protein denaturation, electrophoresis must be carried out in a low-temperature environment of 2℃-8℃, requiring multiple changes of the ice bath. During the ice and solution changes, the protein sample is easily contaminated by the external environment; 4. After the gel strip is cut into pieces, its various shapes are not conducive to protein elution; 5. Filtration of gel residue is very difficult.

[0003] To address the above problems, the present invention provides a protein gel extraction unit with a simple electrophoresis process, high electrophoresis efficiency, and high protein purity, as well as an electrophoretic extraction device containing the unit. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a protein gel extraction unit and an electrophoretic extraction device containing the unit, which has a simple electrophoresis process, high electrophoresis efficiency and high protein purity.

[0005] To solve the above problems, the technical solution adopted by the present invention is as follows:

[0006] A protein gel extraction unit includes a tray body with an annular closed electrophoresis tank on its upper surface, forming a protrusion in the middle and a convex ring on the outside. Before electrophoresis, the protein gel is cut into multiple strips, and the length of the electrophoresis tank is such that several strips are coiled sequentially inside the tank to form an annular closure. The height and width of the electrophoresis tank are such that the electrophoresis solution can completely submerge the strips and that the tank can hold the required volume of electrophoresis solution. An annular closed first electrode and a second electrode are respectively disposed on the first and second sidewalls of the electrophoresis tank, and an electrophoretic electric field is formed between the first and second electrodes.

[0007] In one embodiment of the present invention, a plurality of adhesive strips of equal length are arranged in a disc in an electrophoresis tank, with the strips connected end to end or with gaps between them.

[0008] In one embodiment of the present invention, the first electrode and the second electrode are both conductive wires or conductive plates. When conductive wires are used, a first annular closed groove is formed on the side wall of the electrophoresis tank corresponding to the conductive wires, and the conductive wires are embedded in the first grooves. When conductive plates are used, the conductive plates are fixed in annular closed form on the side wall of the corresponding electrophoresis tank.

[0009] In one embodiment of the present invention, the conductive wire is a metal wire, and the conductive plate is a copper plate, a 316 stainless steel plate, a graphite plate, or a metal matrix composite material with a dense graphite coating on its surface; when the conductive wire is located at the anode, it is a platinum wire, and when the conductive plate is located at the anode, it is a graphite plate or a metal matrix composite material with a dense graphite coating on its surface.

[0010] In one embodiment of the present invention, the first electrode is an anode, which is made of a conductive wire or a conductive plate; the second electrode is a cathode, which is made of a conductive plate.

[0011] In one embodiment of the present invention, a first electrode head and a second electrode head are respectively provided on the boss and the ring on the tray body. The first electrode is connected to the first electrode head through a first wire, and the second electrode is connected to the second electrode head through a second wire. The first electrode head and the second electrode head are respectively connected to the two poles of the power supply.

[0012] An electrophoretic extraction device includes multiple protein gel extraction units as described above, wherein the first electrodes of the multiple protein gel extraction units are connected in parallel and then connected to a power source, and the second electrodes of the multiple protein gel extraction units are connected in parallel and then connected to a power source.

[0013] In one embodiment of the present invention, the height of the first sidewall of the electrophoresis tank is higher than the height of the second sidewall;

[0014] A hollow electrode post is vertically embedded in the upper surface of the tray body on both the boss and the ring. The electrode post is vertically inserted through the tray body. A first electrode head and a second electrode head are detachably fixed on the electrode post on the boss and the ring, respectively.

[0015] Multiple tray bodies are stacked vertically together, and the first electrode head and the second electrode head on the lower tray body are respectively connected to the electrode posts corresponding to the first electrode head and the second electrode head on the upper tray body.

[0016] As one embodiment of the present invention, it further includes a base and a cover plate respectively disposed at the lower end of a plurality of protein gel extraction units. The base and the cover plate are respectively provided with a plurality of corresponding first connecting holes and second connecting holes. The base and the cover plate are connected together by a connecting rod and a nut. The cover plate is provided with a through hole corresponding to the first electrode head and an arc groove corresponding to the second electrode head.

[0017] In one embodiment of the present invention, the tray body is cylindrical, the electrophoresis tank is annular and coaxially arranged with the tray body, and the first electrode head is fixed at the center of the tray body; the tray body and the base are rotatably connected together.

[0018] The electrode post corresponding to the second electrode head protrudes downward from the bottom surface of the tray body, and the upper surface of the base is provided with an annular third clearance groove corresponding to the lower end of the electrode post on the outer side of the tray body.

[0019] The through hole is located in the middle of the cover plate, and multiple arc-shaped grooves are evenly distributed around the through hole.

[0020] The beneficial effects of adopting the above technical solution are as follows:

[0021] The protein gel extraction unit provided by this invention eliminates the need to cut the gel strips into smaller pieces during use. The gel strips can be directly coiled in the electrophoresis tank, with one or more strips placed in the same tank forming a closed loop. This reduces the short-circuit cross-section of the electrophoresis solution between the two electrodes, and the small distance between the first and second electrodes allows proteins to migrate out from the same side of the gel strip, resulting in rapid aggregation, low energy consumption, and significantly improved electrophoresis efficiency. Protein migration can be completed in a maximum of two hours without the need for solution replacement, resulting in high protein purity. After electrophoresis, the gel strips are removed, the electrophoresis solution is aspirated, and the target protein solution can be obtained after concentration or dialysis.

[0022] An electrophoretic extraction device is also provided, which connects multiple protein gel extraction units in parallel for electrophoresis, allowing simultaneous electrophoresis of multiple gel strips. When multiple protein gel extraction units are stacked together, power is directly applied to the first and second electrode heads of the topmost protein gel extraction unit. This not only saves space but also simplifies the circuit connection. The stacked protein gel extraction units are secured between the base and cover plate using a base, cover plate, and connecting rod, resulting in a stable structure and facilitating transfer to a freezer for cryogenic electrophoresis. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the structure of the present invention.

[0024] Figure 2 This is a schematic diagram of the exploded structure from the main perspective of the present invention.

[0025] Figure 3 This is an exploded structural diagram of the present invention from an upward perspective.

[0026] Figure 4 This is a top-view structural diagram of the protein gel extraction unit in this invention.

[0027] Figure 5 yes Figure 4 A magnified view of a portion of point A in the middle.

[0028] Figure 6 This is a schematic diagram of the protein gel extraction unit in this invention from an overhead view.

[0029] Figure 7 This is a top-view structural diagram of the base in this invention.

[0030] Figure 8 This is a schematic diagram of the base structure from an upward angle in this invention.

[0031] Figure 9 This is a top-view structural diagram of the cover plate in this invention.

[0032] Figure 10 This is a schematic diagram of the cover plate from an upward perspective in this invention.

[0033] The components are as follows: 1. Base, 2. First connecting hole, 3. First clearance groove, 4. Second clearance groove, 5. Third clearance groove, 6. Weight reduction hole, 7. Tray body, 701. Boss, 702. Boss ring, 8. Electrophoresis tank, 801. First side wall, 802. Second side wall, 9. First groove, 10. First electrode, 11. First wire, 12. Second electrode, 13. Second groove, 14. Filler, 15. Electrode post, 16. First electrode head, 17. Second electrode head, 18. Second wire, 19. Protrusion, 1901. First chamfer, 20. Cover plate, 2001. First positioning groove, 2002. Second positioning groove, 21. Second connecting hole, 22. Arc groove, 23. Through hole, 2301. Second chamfer, 24. Connecting rod, 25. Nut. Detailed Implementation

[0034] To make the objectives, technical solutions, and advantages of the present invention clearer, the invention will be described clearly and completely below in conjunction with specific embodiments.

[0035] like Figures 4-6 The illustrated protein gel extraction unit includes a tray body 7. An annular closed electrophoresis tank 8 is disposed on the upper surface of the tray body 7. The electrophoresis tank 8 can be circular, triangular, quadrilateral, or other polygonal annular shapes. The electrophoresis tank 8 forms a protrusion 701 in the middle of the tray body 7 and a convex ring 702 on the outside. The sidewall of the electrophoresis tank 8 near the protrusion 701 is defined as the first sidewall 801, and the sidewall near the convex ring 702 is defined as the second sidewall 802. An annular closed first electrode 10 and a second electrode 12 are respectively disposed on the first sidewall 801 and the second sidewall 802 along the length of the electrophoresis tank 8. An electrophoretic electric field is formed between the first electrode 10 and the second electrode 12. Under the action of the electrophoretic electric field, the protein in the gel strip migrates towards the anode side of the first sidewall 801 and the second sidewall 802. Before electrophoresis, the protein gel is usually cut into multiple strips of equal width and length using a gel cutting blade. The length of the electrophoresis tank 8 is set appropriately so that one or more strips are coiled in the electrophoresis tank 8 in sequence to form a closed ring. Preferably, the strips are coiled end to end in the electrophoresis tank 8. There can be gaps between adjacent strips, but they should be avoided from overlapping. The smaller the gap, the better. No gap is the ideal state, which can minimize the electric field cross-section between the two electrodes of the electrophoresis tank 8 that is short-circuited by the electrophoresis liquid.

[0036] The height and width of the electrophoresis tank 8 are reasonably set so that the electrophoresis solution can completely submerge the gel strip and that the tank can hold the required volume of electrophoresis solution. Specifically, the gel strip is in a near-suspended state in the electrophoresis solution. To ensure that the gel strip is completely submerged, the height of the electrophoresis solution in the tank 8 should be slightly higher than the height of the gel strip after it is placed in the tank 8, so that the gel strip can be completely immersed in the electrophoresis solution. The width of the electrophoresis tank 8 is set such that, while meeting the buffer capacity of the electrophoresis solution required for gel strip electrophoresis, the amount of electrophoresis solution placed should be minimized, and the width of the tank 8 should be minimized. The smaller the width of the tank 8, the lower the voltage required for electrophoresis, which can effectively reduce energy consumption. The less electrophoresis solution, the less time is spent on the next step of protein concentration or dialysis in the electrophoresis solution, which can effectively improve work efficiency.

[0037] The protein gel extraction unit does not require cutting the gel strips during use; the strips can be directly coiled inside the electrophoresis tank 8. The circumference of the electrophoresis tank 8 is designed according to the length of the gel strips. When one gel strip is placed in one electrophoresis tank 8, it is best if the two ends of the strip are just joined together. When multiple gel strips are placed in one electrophoresis tank 8, it is best if the multiple strips are joined end to end to form a closed ring shape. This reduces the short-circuit cross-section of the electrophoresis solution between the two electrodes of the electrophoresis tank 8. Furthermore, the small distance between the first electrode 10 and the second electrode 12 allows proteins to migrate out from the same side of the gel strip, resulting in a fast aggregation speed, low energy consumption, and greatly improved electrophoresis efficiency. Protein migration can be completed in a maximum of two hours without changing the solution, resulting in high protein purity. After electrophoresis, the gel strip is removed, the electrophoresis solution is aspirated, and the target protein solution can be obtained after concentration or dialysis.

[0038] In this embodiment, the first electrode 10 is a conductive wire or a conductive plate, and the second electrode 12 is also a conductive wire or a conductive plate. When a conductive wire is used, a first annular closed groove 9 is formed on the side wall of the electrophoresis tank 8 corresponding to the conductive wire along the length direction of the electrophoresis tank 8. The conductive wire is embedded in the first groove 9. In this embodiment, the first groove 9 is a V-shaped groove that opens outward. When a conductive plate is used, the conductive plate is fixed in an annular closed manner on the side wall of its corresponding electrophoresis tank 8.

[0039] In one embodiment, the first electrode 10 is a conductive wire disposed on the inner sidewall of the electrophoresis tank 8, and the second electrode 12 is a conductive plate disposed on the outer sidewall of the electrophoresis tank 8. In other embodiments, the first electrode 10 can be a conductive plate disposed on the inner sidewall of the electrophoresis tank 8, and the second electrode 12 can be a conductive wire disposed on the outer sidewall of the electrophoresis tank 8; the first electrode 10 and the second electrode 12 can also be both conductive wires or conductive plates.

[0040] In this embodiment, the conductive wire is a metal wire, and the conductive plate is a copper plate, a 316 stainless steel plate, a graphite plate, or a metal matrix composite material with a dense graphite coating on its surface. To prevent oxidation, when the conductive wire is located at the anode, a platinum wire is used. A platinum plate is not used because platinum is expensive. When the conductive plate is located at the anode, a graphite plate or a metal matrix composite material with a dense graphite coating on its surface is preferred; a 316 stainless steel plate can also be used to prevent oxidation. In this embodiment, the second electrode 12 is the cathode and uses a conductive plate; the first electrode 10 is the anode, preferably a graphite plate or a metal matrix composite material with a dense graphite coating on its surface. The combination of the graphite plate or the metal matrix composite material with a dense graphite coating and the conductive plate of the second electrode 12 results in a more uniform electric field, which is superior to that of a platinum wire. However, graphite is too brittle and difficult to process; therefore, a platinum wire is used for the first electrode 10 during production.

[0041] The tray body 7 has a first electrode head 16 and a second electrode head 17 respectively disposed on the boss 701 and the convex ring 702. The first electrode 10 is connected to the first electrode head 16 through a first wire 11, and the second electrode 12 is connected to the second electrode head 17 through a second wire 18. The first electrode head 16 and the second electrode head 17 are respectively connected to the two poles of a power supply. In this embodiment, the power supply is a DC power supply, and the first electrode 10 and the second electrode 12 are respectively connected to the positive and negative poles of the power supply.

[0042] A hollow electrode post 15 is vertically embedded in the upper surface of the tray body 7, with an interference fit on the boss 701 and the convex ring 702. The electrode post 15 is vertically inserted through the tray body 7. The first electrode head 16 and the second electrode head 17 are detachably fixed on the two electrode posts 15.

[0043] When the first electrode 10 is a conductive wire disposed on the inner side wall of the electrophoresis tank 8, and the second electrode 12 is a conductive plate disposed on the outer side wall of the electrophoresis tank 8, in order to facilitate the arrangement of the first wire 11, a second groove 13 is provided on the tray body 7 between the first electrode head 16 and the first electrode 10. The second groove 13 is connected to the first groove 9. The second groove 13 is L-shaped, with the horizontal part opened along the radial direction on the upper surface of the tray body 7 and the vertical part opened along the vertical direction on the inner wall of the electrophoresis tank 8. The horizontal part can be a V-shaped groove with the opening facing upward, and the vertical part can be a V-shaped groove with the opening facing outward. The first wire 11 is placed in the second groove 13 and its two ends are respectively connected to the electrode posts 15 corresponding to the first electrode 10 and the first electrode head 16. Then, the second groove 13 is sealed by the filler 14 to prevent the electrophoretic liquid from seeping in. At this time, the first electrode head 16 is connected to the positive terminal of the power supply, and the second electrode head 17 is connected to the negative terminal of the power supply, so that the protein in the gel strip gathers from the side of the second electrode 12 to the side of the first electrode 10. When setting the gel strip, the gel strip is set in the electrophoresis tank 8 on the side close to the second electrode 12.

[0044] like Figures 1-3 As shown, the present invention also provides an electrophoretic extraction device, which includes a plurality of the above-mentioned protein gel extraction units, wherein the first electrodes 10 of the plurality of protein gel extraction units are connected in parallel and then connected to a power source, and the second electrodes 12 of the plurality of protein gel extraction units are connected in parallel and then connected to a power source.

[0045] In a preferred embodiment, multiple tray bodies 7 are vertically stacked together. The first electrode head 16 and the second electrode head 17 on the lower tray body 7 are respectively connected to the electrode posts 15 corresponding to the first electrode head 16 and the second electrode head 17 on the upper tray body 7. The electrode posts 15 are provided with internal threads on their upper parts. The first electrode head 16 and the second electrode head 17 are screwed onto the upper part of the electrode post 15 by external threads on their surfaces. The first electrode head 16 and the second electrode head 17 on the lower tray body 7 are respectively inserted into the electrode posts 15 corresponding to the first electrode head 16 and the second electrode head 17 on the upper tray body 7, thereby realizing the parallel connection of the first electrodes 10 and the parallel connection of the second electrodes 12 in multiple protein gel extraction units.

[0046] The height of the first sidewall 801 of the electrophoresis tank 8 is higher than the height of the second sidewall 802. This arrangement not only creates a gap between the exteriors of the adjacent protein gel extraction units, which is beneficial for heat dissipation in the electrophoresis tank 8, but also prevents the electrophoretic solution in the electrophoresis tank 8 from flowing to the upper surface of the protrusion 701 and coming into contact with the first electrode head 16, causing a short circuit. At the same time, it prevents the electrophoretic solution from entering the upper surface of the protrusion 701 and causing the upper surface of the protrusion 701 to stick together with the bottom of the upper extraction unit and become difficult to separate.

[0047] like Figures 7-9As shown, as a further optimization, the electrophoretic extraction device also includes a base 1 at the lower end and a cover plate 20 at the upper end of the plurality of protein gel extraction units. The base 1 and the cover plate 20 are respectively provided with a plurality of corresponding first connecting holes 2 and second connecting holes 21, wherein the first connecting holes 2 and the second connecting holes 21 are evenly arranged in a circumferential direction. The base 1 and the cover plate 20 are connected together by a connecting rod 24 and a nut 25. The cover plate 20 is provided with a through hole 23 corresponding to the first electrode head 16 and an arc groove 22 corresponding to the second electrode head 17.

[0048] As a further optimization, the sides of the tray body 7 of the multiple protein gel extraction units are labeled with numbers to facilitate identification according to the arrangement order of the gel strips on the protein gel.

[0049] As a further optimization, the lower end of the first connecting hole 2 of the base 1 is provided with a first clearance groove 3 for accommodating the nut 25, and the bottom of the base 1 is also provided with a weight reduction hole 6.

[0050] Multiple protein gel extraction units can be connected in parallel for electrophoresis, allowing simultaneous electrophoresis of multiple gel strips. When multiple protein gel extraction units are stacked together, power can be directly applied to the first electrode head 16 and the second electrode head 17 on the topmost protein gel extraction unit, saving space and simplifying circuit connections. The stacked protein gel extraction units can be fixed between the base 1 and the cover plate 20 using the base 1, the cover plate 20, and the connecting rod 24, resulting in a stable structure and facilitating transfer to a refrigerator for cryogenic electrophoresis.

[0051] In a preferred embodiment, the tray body 7 of the electrophoretic extraction device is cylindrical, the electrophoresis tank 8 is annular and coaxially arranged with the tray body 7, and the first electrode head 16 is fixed at the center of the tray body 7; the tray body 7 and the base 1 are rotatably connected together; specifically, the bottom of the tray body 7 is fixed with a cylindrical protrusion 19 whose contour corresponds to the inner wall of the electrophoresis tank 8, and the middle of the base 1 is provided with a second clearance groove 4 that matches the protrusion 19. At this time, the electrode post 15 is vertically arranged through the tray body 7 and the protrusion 19.

[0052] The electrode post 15 corresponding to the second electrode head 17 protrudes downward from the bottom surface of the tray body 7. The upper surface of the base 1 is provided with an annular third relief groove 5 corresponding to the lower end of the outer electrode post 15 of the tray body 7 outside the second relief groove 4.

[0053] The through hole 23 is located in the middle of the cover plate 20, and multiple arc-shaped grooves 22 are evenly distributed around the through hole 23. The lower part of the through hole 23 is a vertically arranged cylindrical hole, and the upper end is provided with an inverted conical second chamfer 2301, which provides a larger clamping space when the alligator clip is used to connect the first electrode head 16 to the power supply.

[0054] This configuration allows the protein gel extraction units to be rotatably engaged with each other via the protrusion 19 and the second clearance groove 4. When installing the cover plate 20, it is convenient to adjust the angle of the protein gel extraction units so that the second electrode head 17 of the uppermost protein gel extraction unit can smoothly pass through the arc groove 22. The arc groove 22 is connected to the electrophoresis tank 8 of the uppermost protein gel extraction unit, which is beneficial for heat dissipation.

[0055] As a further optimization, to facilitate the installation and disassembly of the first electrode head 16 and the second electrode head 17, a nut or hexagonal prism is fixed above the external threaded portion at the lower part of the first electrode head 16 and the second electrode head 17, which can be tightened and disassembled by a wrench. At this time, the lower end of the through hole on the protrusion 19 for embedding the electrode post 15 is provided with a conical first chamfer 1901. The lower end of the electrode post 15 is located inside the first chamfer 1901. When the first electrode head 16 on the lower tray body 7 and the corresponding electrode post 15 of the upper first electrode head 16 are inserted together, the first chamfer 1901 is used to accommodate the nut or hexagonal prism on the lower first electrode head 16.

[0056] As a further optimization, such as Figure 10 As shown, a first positioning groove 2001 adapted to the outer diameter of the pallet body 7 is provided at the bottom of the cover plate 20, forming a first-level step. During the assembly of the adhesive lifting unit and the cover plate 20, the cover plate 20 is positioned to ensure concentricity between it and the pallet body 7, thus limiting its movement and preventing it from deviating. The first positioning groove 2001 and the pallet body 7 have a transition fit or a small clearance fit. As a further optimization to improve the positioning effect, a second positioning groove 2002 adapted to the boss 801 on the pallet body 7 is also provided on the first positioning groove 2001, forming a second-level step. The depth of the second positioning groove 2002 is equal to or slightly greater than the height of the boss 801 above the pallet body 7.

[0057] Considering chemical inertness, resistance to low-temperature operating environment, low protein adsorption, resistance to oxidant cleaning and high-pressure steam sterilization, the tray body 7 of the base 1, cover plate 20 and protein gel extraction unit is made of polypropylene, polytetrafluoroethylene or polyetheretherketone.

[0058] Specific usage instructions:

[0059] Cut the protein gel into multiple strips;

[0060] When the length of a single gel strip corresponds to the circumference of the electrophoresis tank 8, each gel strip is coiled within its own electrophoresis tank 8, with the ends of the strip just touching. Multiple gel strips are coiled sequentially on protein gel extraction units numbered from smallest to largest or from largest to smallest, according to their arrangement on the protein gel. When the total length of multiple gel strips, such as two strips, corresponds to the circumference of the electrophoresis tank 8, the two strips are coiled end-to-end within the electrophoresis tank 8. Multiple gel strips are coiled in pairs sequentially on protein gel extraction units numbered from smallest to largest or from largest to smallest, according to their arrangement on the protein gel. When setting up the gel strips, placing them on the side wall of the electrophoresis tank 8 closest to the negative electrode of the power supply facilitates the aggregation of proteins towards the side wall of the positive electrode after they have migrated out of the electrophoresis tank 8.

[0061] Add electrophoresis solution into electrophoresis tank 8;

[0062] Multiple protein gel extraction units are stacked on the base 1 in order of their numbers, and the first electrode 10 and the second electrode 12 on the upper and lower protein gel extraction units are inserted together in sequence to form a parallel circuit.

[0063] Cover the cover plate 20 and connect the base 1 and the cover plate 20 together using the connecting rod 24 and the nut 25;

[0064] After the first electrode head 16 and the second electrode head 17 of the assembled electrophoretic extraction device extending from the cover plate 20 are powered on, the device is placed in a refrigerator for electrophoresis.

[0065] After electrophoresis is completed, remove the electrophoresis extraction device from the refrigerator, disassemble it, remove the gel strip from electrophoresis tank 8 and aspirate the electrophoresis solution. After concentration or dialysis, the target protein solution can be obtained.

[0066] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A protein gel extraction unit, characterized in that: It includes a tray body (7), on the upper surface of which is provided an annular closed electrophoresis tank (8), forming a boss (701) in the middle of the tray body (7) and a convex ring (702) on the outside; before electrophoresis, the protein gel is cut into multiple strips, and the length of the electrophoresis tank (8) is such that several strips are coiled in the electrophoresis tank (8) in sequence to form an annular closure; the height and width of the electrophoresis tank (8) are such that the electrophoresis liquid can completely immerse the strips and that the electrophoresis tank (8) can hold the electrophoresis liquid required for electrophoresis; the first sidewall (801) and the second sidewall (802) of the electrophoresis tank (8) are respectively provided with an annular closed first electrode (10) and a second electrode (12), and an electrophoretic electric field is formed between the first electrode (10) and the second electrode (12).

2. The protein gel extraction unit according to claim 1, characterized in that: Multiple strips of equal length are arranged in a disc in an electrophoresis tank (8), with the strips connected end to end or with gaps between them.

3. The protein gel extraction unit according to claim 1, characterized in that: The first electrode (10) and the second electrode (12) are both conductive wires or conductive plates. When a conductive wire is used, the electrophoresis tank (8) has a first annular closed groove (9) on the side wall corresponding to the conductive wire, and the conductive wire is embedded in the first groove (9). When a conductive plate is used, the conductive plate is fixed in an annular closed manner on the side wall of its corresponding electrophoresis tank (8).

4. The protein gel extraction unit according to claim 3, characterized in that: The conductive wire is a metal wire, and the conductive plate is a copper plate, a 316 stainless steel plate, a graphite plate, or a metal matrix composite material with a dense graphite coating on the surface; when the conductive wire is located at the anode, it is a platinum wire, and when the conductive plate is located at the anode, it is a graphite plate or a metal matrix composite material with a dense graphite coating on the surface.

5. The protein gel extraction unit according to claim 4, characterized in that: The first electrode (10) is the anode and is made of a conductive wire or a conductive plate; the second electrode (12) is the cathode and is made of a conductive plate.

6. A protein gel extraction unit according to any one of claims 1-5, characterized in that: The tray body (7) is provided with a first electrode head (16) and a second electrode head (17) on the boss (701) and the convex ring (702), respectively. The first electrode (10) is connected to the first electrode head (16) through the first wire (11), and the second electrode (12) is connected to the second electrode head (17) through the second wire (18). The first electrode head (16) and the second electrode head (17) are respectively connected to the two poles of the power supply.

7. An electrophoretic extraction device, characterized in that: It includes multiple protein gel extraction units as described in any one of claims 1-6, wherein the first electrodes (10) of the multiple protein gel extraction units are connected in parallel and then connected to a power source, and the second electrodes (12) of the multiple protein gel extraction units are connected in parallel and then connected to a power source.

8. The electrophoretic extraction apparatus according to claim 7, characterized in that: The height of the first sidewall (801) of the electrophoresis tank (8) is higher than the height of the second sidewall (802); A hollow electrode post (15) is vertically embedded in the upper surface of the tray body (7) on the boss (701) and the convex ring (702). The electrode post (15) vertically penetrates the tray body (7). A first electrode head (16) and a second electrode head (17) are respectively detachably fixed on the electrode post (15) on the boss (701) and the convex ring (702). Multiple tray bodies (7) are stacked vertically together, and the first electrode head (16) and the second electrode head (17) on the lower tray body (7) are connected to the electrode posts (15) corresponding to the first electrode head (16) and the second electrode head (17) on the upper tray body (7), respectively.

9. The electrophoretic extraction apparatus according to claim 8, characterized in that: It also includes a base (1) at the lower end and a cover plate (20) at the upper end of a plurality of protein gel extraction units respectively. The base (1) and the cover plate (20) are respectively provided with a plurality of first connecting holes (2) and second connecting holes (21). The base (1) and the cover plate (20) are connected together by a connecting rod (24) and a nut (25). The cover plate (20) is provided with a through hole (23) corresponding to the first electrode head (16) and an arc groove (22) corresponding to the second electrode head (17).

10. The electrophoretic extraction apparatus according to claim 9, characterized in that: The tray body (7) is cylindrical, the electrophoresis tank (8) is an annular ring coaxial with the tray body (7), and the first electrode head (16) is fixed at the center of the tray body (7); the tray body (7) and the base (1) are rotatably connected together. The electrode post (15) corresponding to the second electrode head (17) protrudes downward from the bottom surface of the tray body (7), and the upper surface of the base (1) is provided with an annular third clearance groove (5) corresponding to the lower end of the outer electrode post (15) of the tray body (7). The through hole (23) is located in the middle of the cover plate (20), and multiple arc-shaped grooves (22) are evenly provided around the through hole (23).

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