Application of 5% amino-oligosaccharin to sorghum smut

Abstract

The invention discloses application of 5% amino-oligosaccharin to sorghum smut. The 5% amino-oligosaccharin can be used as an active component of a pesticide for resisting plant virus diseases. After sorghum is inoculated with smut germs, sorghum seeds are soaked in 5% amino-oligosaccharin with a certain concentration for 12 h at a constant temperature, and after sowing, it is found that the seeds treated by the 5% amino-oligosaccharin can remarkably inhibit smut infection and proliferation and are remarkably superior to those of a distilled water treatment group. Meanwhile, amino-oligosaccharin has no toxic effect on plants. The compound meets the requirements of agricultural sustainable development and environmental protection, can be developed into an antiviral inhibition medicament as an active ingredient, and has an important application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant science protection, and particularly relates to application of 5% amino oligosaccharide to sorghum head smut. BACKGROUND

[0002] Sorghum is an important grain crop in China, and plays a key role in feed supply and specialty brewing industry (such as brewing of Chinese liquor). However, diseases and pests frequently occur in the production of sorghum, among which head smut is a core disease that restricts the yield and quality of sorghum. Sorghum head smut is a systemic soil-borne disease, mainly with winter spores as the transmission carrier. Winter spores can adhere to soil, manure water and seed surface, and can survive for up to 3 years in extreme environments, with strong stress resistance, bringing great challenges to disease control.

[0003] Current prevention and control of sorghum head smut mainly relies on chemical fungicides such as triazole and benzimidazole agents. Although these agents can inhibit the pathogenic bacteria to some extent, they have significant defects. First, the mechanism is single, directly targeting pathogenic bacteria, which can easily lead to drug resistance of pathogenic bacteria, and the control effect decreases year by year. Second, chemical fungicides are difficult to degrade, and are easy to remain in sorghum grains and soil, which not only affects the quality and safety of agricultural products, but also destroys the balance of soil microecology, which is contrary to the concept of green agriculture development. Third, post-sowing spraying or soil treatment of chemical fungicides cannot effectively block the infection of winter spores on the seed surface and in the soil, and the prevention and control is not thorough, which still has a high risk of disease.

[0004] In the field of plant immune control, amino oligosaccharide is a new type of environmentally friendly plant immune fungicide extracted from the shells of marine organisms such as shrimps and crabs, and synthesized through diversified catalytic hydrolysis reaction. Compared with traditional pesticides, it does not directly act on harmful organisms, but stimulates the immune response of crops, enhances the resistance of cell wall to pathogenic bacteria, and has the functions of stress resistance, disease resistance and yield improvement. However, there is no report on the application of amino oligosaccharide to the prevention and control of sorghum head smut. SUMMARY

[0005] The present application aims to provide an application of 5% amino oligosaccharide to sorghum head smut, which can effectively improve the resistance of plants to resist diseases and pests, and is a new method and technology that is economical, efficient, safe and environmentally friendly.

[0006] In order to achieve the above-mentioned purpose, the present application provides the following technical scheme: Application of 5% amino oligosaccharide to sorghum head smut.

[0007] As a preferred, the use concentration of the amino oligosaccharide is 5%.

[0008] As a preferred, the sorghum seeds are soaked in the amino oligosaccharide solution.

[0009] As a preferred option, the sorghum mentioned includes red-tasseled sorghum.

[0010] Another aspect of the present invention is a pesticide for combating plant viral diseases, the active ingredient of which is an amino oligosaccharide.

[0011] As a preferred embodiment, the anti-plant virus includes *Ustilago maydis*, *Tomato Yellow Leaf Curl Virus*, and *Pseudomonas syringae*.

[0012] Working principle and beneficial effects of the present invention: 1. This invention achieves zero incidence of sorghum smut disease even under artificial inoculation conditions by soaking sorghum seeds in 5% amino oligosaccharide for 12 hours. Compared with conventional agents, it provides more thorough control and stronger stability of efficacy.

[0013] 2. Compared with traditional pesticides, this invention does not act directly on harmful organisms, but rather enhances the resistance of the cell wall to pathogens by stimulating the crop's own immune response, thus possessing the effects of stress resistance, disease resistance, and increased yield.

[0014] 3. This invention is a natural crustacean degradation product that can degrade naturally in the soil without pesticide residues, thus avoiding the pollution of sorghum grains, soil and surrounding ecological environment by chemical fungicides, and meeting the requirements of green agricultural product production. Attached Figure Description

[0015] Figure 1 The effect of 5% amino oligosaccharide on induced resistance and growth promotion in sorghum seeds. Detailed Implementation

[0016] The following detailed description illustrates the specific implementation method: Example 1: The antiviral activity of 5% amino oligosaccharide was determined by infecting red sorghum with sorghum smut pathogen.

[0017] 1. Preparation of test reagents Prepare a 5% amino oligosaccharide solution from 95% amino oligosaccharide powder using distilled water. Measure 150 ml of the 5% amino oligosaccharide solution and 150 ml of distilled water as a control.

[0018] 2. Plant materials The sorghum seeds used in the experiment were provided by Moutai Group. 3. Pesticide application treatment Take 50g of uniformly sized, plump, and healthy sorghum seeds and soak them separately in a 5% solution of amino oligosaccharide and distilled water at a seed-to-liquid ratio of 1:5. Then, place the treated seeds in a constant temperature environment of 25℃ for 12 hours, gently stirring every 8 hours to ensure full contact between the seeds and the liquid. Each treatment is repeated 3 times, with 20 seeds per replicate. 4. Virus inoculation method After soaking, the seeds were inoculated with smut pathogen. The inoculation method used in this example was the rolling inoculation method. After disinfecting the seeds with 10% sodium hypochlorite for 10 minutes, the culture dish containing the seeds was gently shaken and cleaned every 1 minute to achieve thorough disinfection. Subsequently, the seeds were rinsed 5 times with sterile water and rolled in dry mycelium powder to ensure that the seed surface was evenly coated with mycelium powder.

[0019] 5. Plant sowing and control efficacy calculation After inoculation, seeds were sown in corresponding plots of the experimental field according to the field plot design. The sowing depth was 3-4 cm to ensure consistent sowing conditions. After sowing, conventional sorghum field management methods were followed, including watering, weeding, and fertilization, with consistent fertilization amount and timing. No other fungicides were applied throughout the growing season. At the sorghum maturity stage, three sorghum plants were selected from each replicate, and their plant height, panicle length, number of leaves, panicle nodes, flag leaf, second leaf from the top, third leaf from the top, and the length and width of the nodes were recorded.

[0020] 6. Results Analysis Depend on Figure 1 It can be seen that 5% amino oligosaccharide inhibits sorghum growth in the early stage, but promotes its growth in the later stage. As shown in Table 1, sorghum treated with 5% amino oligosaccharide showed significantly better results in terms of plant height, number of leaves, flag leaf length, and flag leaf width after inoculation with smut compared with seeds soaked in distilled water. As shown in Table 2, CK was the control group treated with distilled water, with an incidence rate of 59.66%; D was the blank group treated with distilled water without inoculation, with an incidence rate of 5.66%; and T was the experimental group treated with 5% amino oligosaccharide, with an incidence rate of 3.33%.

[0021] Table 1

[0022] Table 2

[0023] Therefore, it can be seen that 5% amino oligosaccharide has good anti-plant virus activity, and the application of 5% amino oligosaccharide to treat sorghum seeds can significantly inhibit the infection and proliferation of smut pathogen.

[0024] It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solution of this invention. These modifications and improvements should also be considered within the scope of protection of this invention, and will not affect the effectiveness of the invention or the practicality of the patent. The scope of protection claimed in this application shall be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. Application of a 5% amino oligosaccharide in sorghum smut.

2. The application according to claim 1, characterized in that, The concentration of the amino oligosaccharide used is 5%.

3. The application according to claim 2, characterized in that, Soak sorghum seeds in an amino oligosaccharide solution.

4. The application according to claim 3, characterized in that, The sorghum mentioned includes red-tasseled sorghum.

5. A pesticide for combating plant viral diseases, characterized in that, The active ingredient is amino oligosaccharide.

6. The pesticide according to claim 5, characterized in that, The anti-plant viruses include *Ustilago maydis*, *Tomato Yellow Leaf Curl Virus*, and *Pseudomonas syringae*.

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