Tooth-strengthening and anti-inflammatory toothpaste and preparation method thereof
By leveraging the synergistic effect of γ-polyglutamic acid and sophorolipids, combined with traditional Chinese medicine extracts, the long-lasting effectiveness and safety of toothpaste in preventing tooth decay and reducing inflammation have been addressed, achieving long-lasting adhesion to the tooth surface and highly efficient cleaning.
Patent Information
- Application Number
- CN202610016781.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-01-07
- Publication Date
- 2026-02-13
AI Technical Summary
Existing toothpastes have drawbacks in preventing tooth decay and gingivitis, including the risk of fluorosis, the potential harm of chemical antibacterial agents, and the short duration of action of herbal ingredients, making it difficult to achieve long-lasting care.
Using γ-polyglutamic acid and sophorolipid as the core ingredients, the toothpaste utilizes the bioadhesive properties of γ-polyglutamic acid to build a long-acting platform, combined with the cleaning and antibacterial properties of sophorolipid, and compounded with herbal extracts to form a toothpaste formula that synergistically prevents tooth demineralization, hardens tooth enamel, and provides long-lasting anti-inflammatory effects.
It achieves long-lasting adhesion of toothpaste ingredients to the tooth surface, continuously releases effective ingredients, prevents demineralization and hardening of tooth enamel, and provides highly effective cleaning, while also having a gentle anti-inflammatory effect, overcoming the shortcomings of traditional toothpaste.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of daily chemical products technology, specifically to a toothpaste formula with functions of tooth hardening, preventing demineralization, long-lasting anti-inflammatory and cleaning. Background Technology
[0002] Dental caries and periodontitis are two of the most common oral diseases, both rooted in tooth demineralization and the accumulation of pathogenic bacteria. Current anti-caries toothpastes primarily rely on fluoride, which repairs early caries by promoting remineralization. However, there is a risk of fluorosis from overuse of fluoride, and some consumers prefer non-fluoride products.
[0003] In combating dental plaque and gingivitis, commonly used chemical antibacterial agents such as triclosan pose a potential risk of causing oral flora imbalance and bacterial resistance. Herbal extracts (such as honeysuckle and peppermint) are favored for their natural and safe antibacterial and anti-inflammatory properties, but their effect in the oral cavity is short-lived and easily washed away by saliva, making long-lasting care difficult.
[0004] γ-Polyglutamic acid (γ-PGA) is a natural biopolymer with good water solubility, biocompatibility, and biodegradability. Its molecular chain contains a large number of carboxyl groups, giving it extremely strong bioadhesion to hydroxyapatite on the tooth surface. However, current technologies rarely utilize it as a core adhesive carrier to prolong the residence time of herbal ingredients on the tooth surface while simultaneously leveraging its own function of preventing tooth demineralization.
[0005] Sophorolipids are microbial biosurfactants with excellent emulsifying, solubilizing, and antibacterial properties, and are readily biodegradable and environmentally friendly. However, in toothpaste, they are usually used as an auxiliary cleaning ingredient, and their synergistic effect with γ-PGA in protecting tooth enamel and reducing inflammation has not been fully explored.
[0006] Therefore, developing a multifunctional toothpaste that can adhere to the tooth surface for a long time, continuously release effective ingredients, and at the same time harden tooth enamel, prevent demineralization, effectively clean, and gently reduce inflammation is of great practical significance. Summary of the Invention
[0007] The purpose of this invention is to provide a novel toothpaste formula that addresses the shortcomings of existing technologies. This formula uses γ-polyglutamic acid and sophorolipids as its core components. It leverages the bioadhesive properties of γ-polyglutamic acid to construct a long-lasting action platform, combines the cleaning and antibacterial properties of sophorolipids, and incorporates specific herbal extracts to achieve a synergistic effect of preventing tooth demineralization, hardening tooth enamel, and providing long-lasting anti-inflammatory benefits.
[0008] To achieve the above-mentioned technical objectives, the present invention provides a tooth-strengthening and anti-inflammatory toothpaste, comprising the following components by weight percentage: γ-polyglutamic acid 0.1%~5%; sophorolipid 0.5%~8%; traditional Chinese medicine compound extract 1.0%~10%; abrasive 15%~35%; humectant 20%~40%; binder 0.2%~2.0%; foaming agent 1.0%~3.0%; sweetener 0.1%~0.5%; edible flavor 0.8%~1.5%; preservative 0.1%~0.3%; and the balance being deionized water.
[0009] The toothpaste, by weight percentage, contains the following components: 2-4% γ-polyglutamic acid; 2%-5% sophorolipid; 3-6% herbal extract; 25-30% abrasive; 25%-35% humectant; 0.8%-1.5% binder; 1.0%-3.0% foaming agent; 0.2%-0.4% sweetener; 1.0%-1.3% flavoring; 0.1%-0.3% preservative; and the balance being deionized water.
[0010] Specifically, the herbal compound extract is composed of honeysuckle extract, purslane extract and peppermint extract, and the weight ratio of the three is (1~3):(0.5~2):(0.5~2), which has anti-inflammatory, antibacterial and heat-clearing functions.
[0011] The γ-polyglutamic acid is a solid powder with a purity ≥92% and a molecular weight of 500 kDa to 2000 kDa. Preferably, the molecular weight of the γ-polyglutamic acid is 1000 kDa.
[0012] The sophorolipid is a liquid with a purity of ≥40%.
[0013] The abrasive is any one or a combination of several of hydrated silica, calcium carbonate, and dicalcium phosphate; and / or the humectant is any one or a combination of several of sorbitol, glycerin, and propylene glycol; and / or the adhesive is any one or a combination of several of sodium carboxymethyl cellulose, carrageenan, and xanthan gum; and / or the foaming agent is any one or a combination of several of sodium lauroyl sarcosinate and sodium lauryl sulfate.
[0014] The abrasive is a mixture of hydrated silica and calcium bicarbonate in a mass ratio of (3~5):(1~2); and / or the moisturizer is a mixture of glycerin and sorbitol in a volume ratio of (1~2):(1:2); and / or the adhesive is a mixture of sodium carboxymethyl cellulose and sodium carboxymethyl cellulose in a mass ratio of 2~3:1.
[0015] The edible flavoring is any one or a combination of several of the following: peppermint oil, spearmint oil, lime oil, lemon oil, orange oil, clove oil, jasmine oil, cherry blossom oil, and peppermint oil. The preservative is one or a combination of several of the following: potassium sorbate, phenoxyethanol, propylparaben, methylisothiazolinone, hexanediol, and sodium dehydroacetate.
[0016] The present invention further proposes a method for the above-mentioned tooth-strengthening and anti-inflammatory toothpaste, comprising the following steps: (1) Mix the deionized water and moisturizer in the ointment-making pot according to the formula; (2) Add the binder while stirring to allow it to swell fully and form a uniform colloid; (3) Add the formulated amounts of γ-polyglutamic acid, sophorolipid and herbal extract in sequence, and stir at high speed to ensure complete dissolution and dispersion; (4) Add the prescribed amount of friction agent and stir well; (5) Add the foaming agent, sweetener, preservative and edible flavoring according to the formula, and continue to stir until the paste is uniform; (6) After vacuum degassing, the toothpaste is filled to obtain the toothpaste.
[0017] In step (1), the stirring speed is 300-350 rpm, and the stirring is carried out at 35-37℃ for 15-20 minutes; in step (2), the stirring speed is 400-450 rpm, and the stirring is carried out for 25-40 minutes; in step (3), the stirring speed is 500-550 rpm, and the stirring is carried out for 15-20 minutes; in step (4), the stirring speed is 600-650 rpm, and the stirring is carried out for 60-100 minutes to stabilize the viscosity at 4500~6000 mPa·s; in step (5), the stirring speed is 300-250 rpm, and the stirring is carried out for 20-30 minutes.
[0018] Beneficial effects: Compared with the prior art, this application has the following technical effects: Bioadhesion and sustained release: The carboxyl groups in γ-PGA molecules strongly coordinate with calcium ions in tooth enamel, allowing them to firmly adhere to the tooth surface and form a transparent protective film. This film encapsulates and fixes the active ingredients of traditional Chinese medicine, releasing them slowly over time, greatly prolonging their anti-inflammatory effect and overcoming the drawback of traditional Chinese medicine toothpaste being easily washed away by saliva.
[0019] Preventing demineralization and promoting hardening: γ-PGA can adsorb calcium ions in saliva through its carboxyl groups, forming a local calcium ion enrichment area on the tooth surface. When the oral pH value rises (after eating), it can effectively promote the redeposition of calcium ions and apatite, inhibit the demineralization process of teeth, and repair early demineralized areas, thus playing a role in hardening teeth.
[0020] Highly efficient cleaning and penetration: As a biosurfactant, sophorolipids can effectively reduce surface tension, penetrate and break down the biofilm structure of dental plaque, making it easier to remove by physical friction, with a cleaning efficiency higher than that of ordinary surfactants.
[0021] Synergistic antibacterial and anti-inflammatory effects: Sophorolipids themselves have inhibitory and destructive effects on oral pathogens (such as Streptococcus mutans). Their ability to disrupt the bacterial film synergistically works with the antibacterial components of traditional Chinese medicine extracts, enhancing the overall anti-inflammatory effect. Simultaneously, their gentle nature avoids the irritation to the oral mucosa caused by traditional chemical surfactants.
[0022] Synergistic effect: The combined use of γ-PGA and sophorolipids produces a "1+1>2" effect. The adhesion layer constructed by γ-PGA provides a "base" for sophorolipids and traditional Chinese medicine ingredients, while the cleaning and penetration effects of sophorolipids help γ-PGA and traditional Chinese medicine ingredients to work more deeply into the plaque. Together, they construct a multi-layered protection system of "adhesion-cleaning-repair-antibacterial". Detailed Implementation
[0023] The present invention will be further described below with reference to specific embodiments, but the specific embodiments do not limit the present invention in any way.
[0024] Honeysuckle extract (quality requirements conform to standard T / CCCMHPIE 1.11-2016), purslane extract (quality requirements conform to standard T / CNHFA 111.121-2024), peppermint extract (quality requirements conform to standard GB 1886.204-2016). Example
[0025] This embodiment discloses a toothpaste for strengthening teeth and reducing inflammation, the formulation of which is shown in Table 1 below: Table 1
[0026] The toothpaste for strengthening teeth and reducing inflammation described in this embodiment is prepared as follows: Preparation stage: Accurately weigh each component according to the formula ratio, pass the friction agent through an 80-mesh sieve, and place γ-polyglutamic acid and sophorolipid in a constant temperature environment of 25℃ for 1 hour in advance to equilibrate. Premixing the moisturizing system: Add deionized water and moisturizer to the ointment mixing pot, turn on the stirrer, turn the speed to 350 rpm, heat to 37℃, stir for 15 minutes until uniform and transparent; Colloidal formation: While stirring, slowly add the binder, increase the speed to 400 rpm, and stir for 30 minutes until the binder is completely swollen and forms a transparent colloid without visible particles. Mixing of core ingredients: Add γ-polyglutamic acid, sophorolipid, and compound extract of traditional Chinese medicine in sequence. Adjust the speed to 500 rpm, turn on the high-speed homogenization function, and stir for 20 minutes. Use a laser particle size analyzer to monitor and ensure that the dispersed particle size is ≤3μm. Friction agent mixing: Add friction agent, reduce the speed to 650 rpm, stir for 60 minutes, take a sample every 15 minutes during the period, and monitor the viscosity of the paste at 25℃ using a viscometer to ensure that the viscosity is stable at 4500~6000 mPa·s; Mixing of excipients: Add foaming agent, sweetener, preservative, and food flavoring, reduce the speed to 250 rpm, stir for 20 minutes, until the paste has a uniform color and smell; Post-processing: The paste is degassed under vacuum at a vacuum level of -0.09 MPa for 23 minutes; after cooling to 25°C, it is filled into toothpaste tubes using an automatic filling machine (filling accuracy ±1%) and then sealed to obtain the finished product.
[0027] Comparative Example 1 (γ-polyglutamic acid content below the protective range) Based on Example 1, for the formulation components, only the γ-polyglutamic acid content was adjusted to 0.05%; for the preparation method, in the core component mixing stage, due to the low γ-polyglutamic acid content, the homogenization stirring time was shortened to 15 minutes.
[0028] The remaining components and preparation methods are the same as in Example 1.
[0029] Comparative Example 2 (γ-polyglutamic acid content higher than the protection range) Based on Example 1, the only adjustment to the formulation components was to adjust the γ-polyglutamic acid content to 6%; for the preparation method, during the colloid formation stage, because γ-polyglutamic acid is prone to agglomeration, the stirring speed was increased to 450 rpm and the stirring time was extended to 35 minutes.
[0030] The remaining components and preparation methods are the same as in Example 1.
[0031] Comparative Example 3 (Sophorolipid content below the protection limit) Based on Example 1, the only adjustment to the formulation components was that sophorolipid was 0.3%; and in the preparation method, the stirring time of the foaming agent was extended to 25 minutes during the mixing stage of excipients because sophorolipid has weak surface activity.
[0032] The remaining components and preparation methods are the same as in Example 1.
[0033] Comparative Example 4 (Sophorolipid content higher than the protection limit) Based on Example 1, the only adjustment to the formulation components was that sophorolipid was 9%; and in the preparation method, during the friction agent mixing stage, because sophorolipid is prone to foaming, the stirring speed was reduced to 600 rpm and the stirring time was extended to 65 minutes.
[0034] The remaining components and preparation methods are the same as in Example 1.
[0035] Comparative Example 5 (γ-polyglutamic acid molecular weight below the protection range) Based on Example 1, for the formulation components, only the γ-polyglutamic acid with a molecular weight of 1000kDa was replaced with γ-polyglutamic acid with a molecular weight of 300kDa; for the preparation method, in the core component mixing stage, since the low molecular weight γ-polyglutamic acid dissolves quickly, the homogenization stirring time is shortened to 18 minutes.
[0036] The remaining components and preparation methods are the same as in Example 1.
[0037] Comparative Example 6 (γ-polyglutamic acid with a molecular weight higher than the protection range) Based on Example 1, for the formulation components, only the γ-polyglutamic acid with a molecular weight of 1000kDa was replaced with γ-polyglutamic acid with a molecular weight of 2500kDa; for the preparation method, in the core component mixing stage, since the high molecular weight γ-polyglutamic acid dissolves slowly, it needs to be pre-dissolved in a small amount of warm water (37°C) before being added to the colloid, and the homogenization stirring time is extended to 30 minutes.
[0038] The remaining components and preparation methods are the same as in Example 1.
[0039] Comparative Example 7 (γ-polyglutamic acid molecular weight is the lower limit of the protection range) Based on Example 1, only the formulation components were adjusted, replacing γ-polyglutamic acid with a molecular weight of 1000kDa with γ-polyglutamic acid with a molecular weight of 500kDa.
[0040] The remaining components and preparation methods are the same as in Example 1.
[0041] The toothpastes of Example 1 and Comparative Examples 1 to 7 were evaluated for their tooth-strengthening effect, anti-inflammatory effect, antibacterial effect, performance, and safety.
[0042] I. Subject Selection Seven groups of subjects were selected, with 30 participants in each group (aged 20-45 years). All participants met the following criteria: no severe periodontal disease, enamel hardness of 260-280 HV, gingival redness and swelling score of 1-2, and oral Streptococcus mutans content of 8×10⁻⁶. 6 ~12×10 6 CFU / mL, using toothpaste from Example 1 and Comparative Examples 1-7 respectively, brushing teeth for 2 minutes each morning and evening for 8 weeks.
[0043] II. Detection Indicators and Methods The relevant detection indicators and methods are shown in Table 2 below: Table 2
[0044] III. Test Results 3.1 Tooth Strengthening Effect The results of the tooth-strengthening efficacy evaluation are shown in Table 3 below. Table 3
[0045] 3.2 Anti-inflammatory effects 3.2.1 Gingival redness and swelling score The results of the gingival redness and swelling assessment are shown in Table 4 below. Table 4
[0046] 3.2.2 Frequency of gingival bleeding per week The results of the weekly gingival bleeding frequency evaluation are shown in Table 5 below. Table 5
[0047] 3.2.3 TNF-α concentration in gingival crevicular fluid The results of the evaluation of TNF-α concentration in gingival crevicular fluid are shown in Table 6 below.
[0048] Table 6
[0049] 3.3 Antibacterial efficacy The results of the antibacterial efficacy evaluation are shown in Table 7 below.
[0050] Table 7
[0051] 3.4 Performance and Safety The performance and safety evaluation results are shown in Table 8 below.
[0052] Table 8
[0053] IV. Results Analysis 4.1 Overall Advantages of the Optimal Implementation Example (Example 1) Example 1 showed the best performance in terms of tooth strengthening, anti-inflammation, antibacterial properties, usability, and safety: tooth enamel hardness increased by 40.1 HV, gingival redness and swelling decreased by 75.0%, pathogenic bacteria decreased by 78.1%, the flora balance rate was 93.3%, and there was no mucosal irritation and a high taste score, which proved that the combination of "γ-polyglutamic acid 2.5% + sophorolipid 3.0% + molecular weight 1000kDa" can achieve synergistic effects of each component.
[0054] 4.2 The impact of scale adjustment (Comparative Examples 1-4) The content of γ-polyglutamic acid in Comparative Example 1 was insufficient, below the protection range (0.05%), and the total amount of carboxyl groups was insufficient, so it could not form a stable adhesive film. The tooth-strengthening, anti-inflammatory and antibacterial effects were significantly reduced, and the hardness increase was only 21.7% of that in Example 1, which confirms that γ-polyglutamic acid needs to reach a certain content to play a role. The content of γ-polyglutamic acid in Comparative Example 2 was too high, exceeding the protection range (6.0%), and the viscosity of the paste was too high (8500 mPa·s), resulting in a poor user experience and no improvement in efficacy (the hardness increase was only 63.1% of that in Example 1). It also caused mucosal irritation, indicating that excessive amounts are meaningless. Comparative Example 3 had insufficient sophorolipid content, below the protection range (0.3%), weak surface activity, insufficient foam (8 mL), poor cleaning power, and the pathogenic bacteria reduction rate was only 71.6% of that in Example 1, confirming that a certain content of sophorolipid is required to break down the bacterial film. Comparative Example 4 had an excessive amount of sophorolipid, exceeding the protection range (9.0%), excessive foam (22 mL), a spicy taste, and an irritation rate of 33.3%. Its efficacy was actually reduced (the redness and swelling reduction rate was only 66.7% of that in Example 1), indicating that sophorolipid needs to be controlled within a reasonable range.
[0055] 4.3 Effect of molecular weight adjustment (Comparative Examples 5-7) The γ-polyglutamic acid in Comparative Example 5 has a molecular weight of 300 kDa, which is too low. The molecular chain is short and the adhesion is weak. The hardness improvement is only 56.4% of that in Example 1. The anti-inflammatory and antibacterial effects also decrease simultaneously, which confirms that low molecular weight cannot meet the requirements for adhesion and sustained release. The γ-polyglutamic acid in Comparative Example 5 has a molecular weight of 2500 kDa, which is too high. The molecular chains are entangled, resulting in poor solubility and dispersibility. The hardness improvement is only 72.0% of that in Example 1, and the efficacy is limited by the uniformity of dispersion. The molecular weight of γ-polyglutamic acid in Comparative Example 7 was 500 kDa, which was within the protection range, but the adhesion duration was short (adhesion duration of 4.2 hours, lower than 6.5 hours in Example 1), and the efficacy was slightly lower than that of Example 1 (hardness increased to 78.5% of Example 1), confirming that 1000 kDa is the optimal molecular weight.
[0056] This invention provides a concept and method for a toothpaste that strengthens teeth and inhibits bacteria. Many methods and approaches exist for implementing this technical solution; the above description is merely a preferred embodiment of the invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this invention, and these improvements and modifications should also be considered within the scope of protection of this invention. All components not explicitly stated in this embodiment can be implemented using existing technologies.
Claims
1. A toothpaste for strengthening teeth and reducing inflammation, characterized in that, By weight percentage, it contains the following components: γ-polyglutamic acid 0.1%~5%; sophorolipid 0.5%~8%; compound extract of traditional Chinese medicine 1.0%~10%; abrasive 15%~35%; humectant 20%~40%; binder 0.2%~2.0%; foaming agent 1.0%~3.0%; sweetener 0.1%~0.5%; edible flavor 0.8%~1.5%; preservative 0.1%~0.3% and the balance deionized water.
2. The toothpaste for strengthening teeth and reducing inflammation according to claim 1, characterized in that, By weight percentage, it contains the following components: γ-polyglutamic acid 2-4%; sophorolipid 2%-5%; compound extract of traditional Chinese medicine 3-6%; abrasive 25-30%; humectant 25%-35%; binder 0.8%-1.5%; foaming agent 1.0%-3.0%; sweetener 0.2%-0.4%; edible flavor 1.0%-1.3%; preservative 0.1%-0.3% and the balance deionized water.
3. The toothpaste for strengthening teeth and reducing inflammation according to claim 1 or 2, characterized in that, The herbal compound extract consists of honeysuckle extract, purslane extract and peppermint extract, with a weight ratio of (1~3):(0.5~2):(0.5~2).
4. The toothpaste for strengthening teeth and reducing inflammation according to claim 1 or 2, characterized in that, The γ-polyglutamic acid is a solid powder with a purity ≥92% and a molecular weight of 500kDa~2000kDa.
5. The toothpaste for strengthening teeth and reducing inflammation according to claim 1 or 2, characterized in that, The sophorolipid is a liquid with a purity of ≥40%.
6. The toothpaste for strengthening teeth and reducing inflammation according to claim 1 or 2, characterized in that, The abrasive is any one or a combination of several of hydrated silica, calcium carbonate, and dicalcium phosphate; and / or the humectant is any one or a combination of several of sorbitol, glycerin, and propylene glycol; and / or the adhesive is any one or a combination of several of sodium carboxymethyl cellulose, carrageenan, and xanthan gum; and / or the foaming agent is any one or a combination of several of sodium lauroyl sarcosinate and sodium lauryl sulfate.
7. The toothpaste for strengthening teeth and reducing inflammation according to claim 1, characterized in that, The abrasive is a mixture of hydrated silica and calcium bicarbonate in a mass ratio of (3~5):(1~2); and / or the moisturizer is a mixture of glycerin and sorbitol in a volume ratio of (1~2):(1:2); and / or the adhesive is a mixture of sodium carboxymethyl cellulose and sodium carboxymethyl cellulose in a mass ratio of 2~3:
1.
8. The toothpaste for strengthening teeth and reducing inflammation according to claim 1, characterized in that, The edible flavoring is any one or a combination of peppermint oil, spearmint oil, lime oil, lemon oil, orange oil, clove oil, jasmine oil, cherry blossom oil, and peppermint oil; and / or the preservative is any one or a combination of potassium sorbate, phenoxyethanol, propylparaben, methylisothiazolinone, hexanediol, and sodium dehydroacetate.
9. A method for preparing a tooth-strengthening and anti-inflammatory toothpaste as described in any one of claims 1 to 7, characterized in that, Includes the following steps: (1) Mix the deionized water and moisturizer in the ointment-making pot according to the formula; (2) Add the binder while stirring to allow it to swell fully and form a uniform colloid; (3) Add the formulated amounts of γ-polyglutamic acid, sophorolipid and herbal extract in sequence, and stir at high speed to ensure complete dissolution and dispersion; (4) Add the prescribed amount of friction agent and stir well; (5) Add the foaming agent, sweetener, preservative and edible flavoring according to the formula, and continue to stir until the paste is uniform; (6) After vacuum degassing, the toothpaste is filled to obtain the toothpaste.
10. The preparation method according to claim 8, characterized in that, In step (1), the stirring speed is 300-350 rpm, and the stirring is carried out at 35-37℃ for 15-20 minutes; in step (2), the stirring speed is 400-450 rpm, and the stirring is carried out for 25-40 minutes; in step (3), the stirring speed is 500-550 rpm, and the stirring is carried out for 15-20 minutes; in step (4), the stirring speed is 600-650 rpm, and the stirring is carried out for 60-100 minutes to stabilize the viscosity at 4500~6000 mPa·s; in step (5), the stirring speed is 300-250 rpm, and the stirring is carried out for 20-30 minutes.