Saline-alkali tolerant phosphate-solubilizing pesticide-reducing fungus YM-1 and application thereof in corn planting

Abstract

The invention relates to the technical field of agricultural production, and discloses a salt-alkali-tolerant phosphorus-dissolving pesticide-reducing fungus YM-1 and application thereof in corn planting, the salt-alkali-tolerant phosphorus-dissolving pesticide-reducing fungus YM-1 is a purple blue bacterium, the preservation number of the strain is CGMCC No.42313, and the strain has multiple functions of resisting salt and alkali, dissolving insoluble phosphorus and degrading 2-methyl-4-chlorophenoxyacetic acid pesticide residues. The strain, a culture thereof or a microbial inoculum prepared from the strain are applied to agricultural production, and plant growth is promoted by exerting the composite biological function of the strain under saline-alkali stress. According to the invention, the synergistic effect of saline-alkaline tolerance, phosphate solubilization and pesticide degradation of the strain is utilized, so that the plant growth inhibition is relieved in the adverse situation, the soil phosphorus bioavailability is improved, the pesticide-polluted soil is repaired, and the plant seed germination, root development and plant growth are promoted. The strain has an important application prospect in the aspect of developing a microbial agent for saline-alkali soil improvement.

Description

Technical Field

[0001] This invention relates to the field of agricultural production technology, specifically to a salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus YM-1 and its application in maize cultivation. Background Technology

[0002] Currently, soil salinization is one of the key factors restricting agricultural development. my country has a large area of ​​saline-alkali land, where high salt content and poor soil structure severely inhibit crop growth. The high pH and abundant metal ions in saline-alkali environments easily combine with phosphorus to form insoluble phosphates that are difficult for plants to utilize, resulting in low soil phosphorus activity. Simultaneously, salt stress slows down the natural degradation process of organic pollutants in the soil; for example, the commonly used herbicide MCPA has a longer residual period in these soils.

[0003] To address the aforementioned need for saline-alkali land improvement, various remediation strategies have been developed using existing technologies. Among these, microbial remediation methods have attracted attention due to their environmental friendliness. This method typically involves screening and applying microorganisms with specific functions. For example, salt-tolerant microorganisms are used to improve soil physicochemical properties, thereby enhancing plant resilience. Alternatively, specialized phosphorus-solubilizing microorganisms are screened to convert unavailable phosphorus in the soil into soluble phosphorus through their metabolic activities, thus improving nutrient supply. Furthermore, specific degradative strains are also screened to remediate pesticide residue pollution in the soil.

[0004] However, existing technologies still have limitations in practical applications. The functional microorganisms currently screened often have relatively singular functions. The survival rate and activity of some phosphorus-solubilizing microorganisms are inhibited in saline-alkali soils, resulting in poor phosphorus solubilization. Conversely, many salt-tolerant microorganisms do not possess efficient nutrient activation capabilities, thus contributing little to improving soil fertility. Single microbial strains capable of simultaneously possessing the combined functions of salt tolerance, phosphorus solubilization, and pesticide residue degradation are rare in current technologies. Therefore, current integrated management of saline-alkali land often requires the combined application of multiple microbial agents, which not only increases costs and operational complexity but also, due to the antagonistic effects between different strains, affects the final improvement effect, making it difficult to achieve efficient synergistic management.

[0005] Therefore, this invention provides a salt- and alkali-tolerant phosphorus-relieving and pesticide-reducing fungus YM-1 and its application in maize cultivation, in order to address the shortcomings of the prior art. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus, YM-1, and its application in maize cultivation. This solves the problem that existing technologies lack a single, highly efficient microbial agent that can simultaneously address the multiple factors such as salt-alkali stress, low soil phosphorus availability, and pesticide residues.

[0007] To achieve the above objectives, the present invention provides the following technical solution: In a first aspect, the present invention provides a salt-tolerant, alkali-resistant, phosphorus-solubilizing, pesticide-reducing fungus YM-1, wherein the salt-tolerant, alkali-resistant, phosphorus-solubilizing, pesticide-reducing fungus YM-1 is a purplish-blue spore-producing fungus. Talaromyces purpureogenus The strain number is YM-1, the accession number is CGMCC No.42313, and it is deposited at the China General Microbiological Culture Collection Center.

[0008] By adopting the above technical solution, this invention provides a microbial strain with multiple functions, including salt and alkali tolerance, dissolution of insoluble phosphorus, and pesticide degradation. Existing microbial agents have relatively limited functions and their activity is often inhibited in stressful soil environments such as saline-alkali soils, making it difficult to simultaneously address the problems of low nutrient availability and pesticide residue pollution. The purplish-blue bacterium YM-1 provided by this invention, due to its unique physiological and biochemical characteristics, can maintain high biological activity in stressful environments with high salinity and a wide pH range.

[0009] This strain can secrete active substances such as organic acids and phosphatases during its metabolism. These substances can undergo acidolysis or complexation reactions with water-insoluble inorganic phosphorus in the soil, or hydrolyze poorly utilized organic phosphorus, ultimately converting these fixed phosphorus sources that plants cannot absorb into soluble phosphate ions, directly improving the bioavailability of soil phosphorus.

[0010] Therefore, this strain provides a core microbial resource for the development of novel microbial agents, and is particularly suitable for improving saline-alkali soil, enhancing nutrient utilization, and remediating pesticide pollution, thus possessing agricultural application value.

[0011] Preferably, a culture comprising the purplish-blue bacterium YM-1 is provided. This culture is obtained by amplifying the strain in a suitable culture medium, containing a high concentration of live bacterial strain.

[0012] By adopting the above technical solution, strain materials for large-scale production were obtained, providing a foundation for the subsequent preparation of microbial agent products.

[0013] Preferably, a bacterial agent containing the said purple-blue bacterium YM-1, its metabolites and / or its culture.

[0014] By employing the above-mentioned technical solution, the bacterial strain, its beneficial metabolites produced during cultivation, and its culture are prepared into a stable, applicable product form. This microbial agent not only contains live bacteria to colonize the soil and exert a continuous effect, but its metabolites also immediately exert biochemical functions such as phosphorus solubilization and pesticide degradation, thereby achieving faster and more efficient field application results. This microbial agent can be a plant growth promoter or a pesticide residue degrader in the soil.

[0015] Secondly, this invention provides an application of the purplish-blue bacterium YM-1, employing the following technical solution: The purple-blue spore-producing bacterium YM-1, its culture, or a bacterial agent containing it, as described in the first aspect, is applied to the field of agricultural production.

[0016] By adopting the above technical solution, this invention establishes an innovative agricultural application model that utilizes a single microorganism to achieve four combined effects: stress relief, nutrient increase, toxin degradation, and growth promotion. After the multifunctional purple-blue bacterium YM-1 is applied to the agricultural ecosystem, this strain can colonize the plant rhizosphere microenvironment and actively exert its complex biological functions. The reaction process includes: Step 1: Application and colonization. The inoculant is introduced through seed dressing, root irrigation, or soil spraying. Thanks to its excellent salt and alkali tolerance, the strain successfully colonizes around the plant roots in stressful soil environments.

[0017] Step Two: Synergistic Effects. After colonization, the strain begins metabolic activity, simultaneously initiating multiple biochemical pathways: Nutrient transformation: It continuously secretes phosphatases and organic acids to convert insoluble phosphorus around the rhizosphere into available phosphorus that can be absorbed by the plant roots, providing key nutrients for the plant.

[0018] Stress relief and toxicity elimination: Simultaneously producing degradation enzymes to decompose MCPA residues in the soil, reducing its growth inhibition and toxic effects on plants, and purifying the rhizosphere environment.

[0019] Step 3: Promote plant growth. Under the dual favorable conditions of increased nutrient supply and reduced environmental stress, the plant's own growth potential is stimulated, which is manifested in increased seed germination rate, more developed root system, and stronger plant.

[0020] Therefore, this application solution systematically solves the complex obstacles in planting on saline-alkali land through a simple biological measure. Compared with the traditional method of applying multiple fertilizers, amendments and remediation agents separately, it is lower in cost, easier to operate and more environmentally friendly.

[0021] Preferably, the application is for degrading organic phosphorus, inorganic phosphorus and / or MCPA pesticide residues in the soil; and / or alleviating salt stress and / or alkali stress in plants.

[0022] By adopting the above technical solutions, it is clear that the technical value of this application lies in soil improvement and environmental remediation, which can effectively enhance soil fertility and reduce chemical pollution.

[0023] Preferably, the application is to promote plant growth, specifically including promoting plant seed germination, increasing seedling emergence rate, increasing plant height, root length and / or biomass.

[0024] By adopting the above technical solutions, the application is directly reflected in the improvement of crop agronomic traits. As shown in the experiment, it can effectively improve the emergence rate, average plant height, shoot length and root length of maize under salt and alkali stress, providing technical support for ensuring grain yield.

[0025] Preferably, the application is carried out under salt stress and / or alkali stress conditions.

[0026] By adopting the above technical solution, the unique competitive advantages and application value of this invention in adverse agricultural environments such as saline-alkali land are highlighted.

[0027] Preferably, the application also includes promoting phosphorus accumulation in plants.

[0028] By adopting the above technical solution, the effect of this application has been confirmed from the perspective of plant physiology. That is, by improving the availability of external phosphorus, the phosphorus content in plant tissues is ultimately increased, providing a sufficient material basis for the healthy growth of plants.

[0029] Preferably, the plant is a grass, and more preferably, corn.

[0030] By adopting the above-mentioned technical solutions, specific and feasible biotechnological solutions have been provided for stabilizing and increasing the yield of major grain crops such as corn in saline-alkali areas.

[0031] This invention provides a salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus, YM-1, and its application in maize cultivation. It has the following beneficial effects: 1. The purple-blue bacterium YM-1 provided by this invention possesses excellent tolerance to salt and alkali stress, promoting plant growth in saline-alkali environments. After being applied to the soil, this strain maintains high biological activity and effectively colonizes the plant rhizosphere even under high salt and high alkalinity stress conditions. Through its own physiological regulatory mechanisms, it can effectively alleviate the inhibitory effects of environmental stress on plant seed germination and seedling growth, resulting in increased crop emergence rate, plant height, and root development, thereby enhancing the crop's adaptability to saline-alkali soils and providing an effective biological solution for agricultural production on marginal lands.

[0032] 2. The strain provided by this invention possesses the ability to efficiently convert insoluble phosphorus, thereby improving the bioavailability of phosphorus in the soil. Through metabolic secretion of organic acids and phosphatases, this strain can decompose and convert fixed inorganic and organic phosphorus sources in the soil, which are difficult for plants to utilize, into soluble and available phosphorus forms. This process directly promotes the absorption and accumulation of the key nutrient phosphorus by plants, reducing dependence on chemical phosphate fertilizers. It not only contributes to robust crop growth but also improves soil fertility structure, aligning with the development direction of green agriculture.

[0033] 3. The strain provided by this invention possesses the function of degrading specific pesticide residues, which helps to restore the soil environment and ensure crop safety. This strain can effectively degrade herbicides such as MCPA remaining in the soil, breaking down toxic pesticide molecules into less toxic or non-toxic substances through its own enzymatic reaction. This function eliminates harmful factors that inhibit plant growth, creating a healthier growing environment for crops, while also reducing the risk of pesticide residues in agricultural products. It is of great significance for restoring the soil microecological balance and achieving sustainable agricultural development. Attached Figure Description

[0034] Figure 1 This is a colony morphology diagram of strain YM-1 of the present invention on a plate; Figure 2 This is a microscopic image of strain YM-1 of the present invention; Figure 3 Phylogenetic tree of strain YM-1 of the present invention; Figure 4 The results of salt tolerance testing for strain YM-1 of this invention; Figure 5 The results of alkali resistance testing for strain YM-1 of this invention are shown. Figure 6 This is a graph showing the organophosphate solubilizing ability of strain YM-1 of the present invention; Figure 7 This is a graph showing the inorganic phosphorus solubility of strain YM-1 of the present invention; Figure 8 The degradation characteristics of the pesticide MCPA by the strain YM-1 of the present invention under salt and alkali stress; Figure 9 The effect of strain YM-1 under salt and alkali stress on maize seed germination according to the present invention; Figure 10 The effect of strain YM-1 under salt and alkali stress on maize growth according to the present invention; Figure 11 This is a graph showing the phosphorus accumulation in the aboveground parts and roots of maize after inoculation with strain YM-1 under salt and alkali stress according to the present invention. Detailed Implementation

[0035] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] Preparation example: Preparation of purple-blue spore-producing bacteria YM-1 This preparation example provides a salt- and alkali-tolerant phosphorus-solubilizing and pesticide-reducing fungus, YM-1, also known as a purple-blue variegated fungus. Talaromyces purpureogenus They obtained a pure culture of the strain.

[0037] Strain Isolation: Weigh 10g of rhizosphere soil sample and add it to 90ml of sterile water containing glass beads. Shake thoroughly at 150r / min for 30min to obtain a rhizosphere soil stock solution bacterial suspension. Dilute the bacterial suspension to 10... -3 10 -4 and 10 -5 Gradients were used, with 100 μL of each culture spread onto PDA agar plates containing 50 mg / kg MCPA. After incubation at 27°C for 5 days, single colonies were selected for streak purification and transferred to glycerol tubes for storage.

[0038] Morphological identification: Colonies of strain YM-1 are slightly raised, fluffy or short, cottony, initially white, turning grayish-green after sporulation (see...). Figure 1 Microscopic examination revealed that its conidiophores were smooth and without apical sacs, and the conidia were elliptical (see...). Figure 2 ).

[0039] Molecular biological identification: Genomic DNA was extracted from strain YM-1, and the ITS gene fragment was amplified by PCR using universal primers ITS1 / 4 and then sequenced. Sequencing results were compared with the NCBI database, showing consistency with... Talaromyces purpureogenus (JX315671.1) showed 99.62% homology. A phylogenetic tree was constructed using MEGA 7.0 software (see...). Figure 3 The strain was ultimately identified as YM-1, a bacterium that produces purple-blue morphology.

[0040] Examples 1-3: Example 1: This embodiment provides the application of a salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus, YM-1, in maize cultivation, aiming to utilize strain YM-1 to promote maize seed germination under salt-alkali stress.

[0041] The strain YM-1 obtained in Preparation Example 1 was inoculated into PDB medium to prepare a solution with a concentration of 1×10⁻⁶. 7 A spore suspension of cfu / mL was prepared. 2 mL of this spore suspension was resuspended in a moderately saline-alkali stress solution (containing 0.15 g / L NaCl, 1.5 g / L Na₂SO₄, 3 g / L NaHCO₃, 0.45 g / L Na₂CO₃, pH 8.5, MCPA concentration 20 mg / L) to obtain the inoculum. This inoculum was then evenly dripped onto sterile filter paper containing 5 corn seeds (Zhengdan 958), ensuring complete saturation.

[0042] Example 2: This embodiment provides the application of a salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus, YM-1, in maize cultivation, aiming to promote maize growth in saline-alkali land using strain YM-1.

[0043] Five uniformly sized corn seeds (Zhengdan 958) were sown in pots filled with German Floragad peat moss, one seed per pot. A 1×10⁻⁶ concentration was prepared according to the method in Example 1. 7 Take 10 mL of a CFU / mL YM-1 spore suspension and evenly drop it onto the seeds, ensuring thorough saturation. Collect the plants after 20 days of culture.

[0044] Example 3: This embodiment provides the application of a salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus, YM-1, in maize cultivation, aiming to promote phosphorus accumulation in maize under salt-alkali stress.

[0045] Corn plants cultured for 20 days in Example 2 were harvested, their roots were rinsed with distilled water, blanched at 105°C for 30 minutes, dried at 75°C to constant weight, and finally ground for later use. The total phosphorus concentration in the aboveground and underground parts of the plants was measured, and the phosphorus accumulation was calculated based on the dry weight of the plants.

[0046] Comparative Examples 1-2: Comparative Example 1: Compared with Example 1, the difference is that when treating corn seeds, 2 mL of a moderately saline-alkali stress solution (without YM-1 spore suspension) was used, otherwise the same.

[0047] Comparative Example 2: Compared with Examples 2 and 3, the difference is that during the potting treatment, 300 mL of moderate salt-alkali stress solution (without YM-1 bacterial solution) was added to 330 g of peat soil; all other aspects were the same.

[0048] Test Examples 1-7: Test Example 1: Salt and Alkali Tolerance Test of Strain YM-1 Experimental description: Salt tolerance test: 2.5%, 5.0%, 7.5%, 10%, 12.5%, and 15.0% NaCl were added to PDA medium, respectively. The strain YM-1 obtained in Preparation Example 1 was inoculated onto the above-mentioned media with different salinities, with three replicates for each treatment. The media were incubated at 27°C and the colony size was observed.

[0049] Alkali tolerance test: The pH of PDA medium was adjusted to 7.0, 8.0, 9.0, 10.0, 11.0, and 12.0 using NaOH solution. Strain YM-1 was inoculated onto the media at these different pH values, with three replicates for each treatment. The media were incubated at 27°C, and colony size was observed.

[0050] Experimental data: The experimental results are qualitative observations, such as... Figure 4 and Figure 5 As shown.

[0051] Experimental conclusions: The results show that strain YM-1 can grow in PDA medium with NaCl content of 2.5%-12.5% ​​(see...). Figure 4 ), and can grow in PDA medium with pH 7-11 (see Figure 5 It exhibits good salt and alkali resistance.

[0052] Test Example 2: Test of the organophosphate solubility of strain YM-1 Experimental description: Organic phosphorus soluble medium: glucose 10.0g; ammonium sulfate 0.5g; yeast extract 0.5g; sodium chloride 0.3g; potassium chloride 0.3g; magnesium sulfate 0.3g; ferrous sulfate 0.03g; manganese sulfate 0.03g; lecithin 0.2g; calcium carbonate 1.0g; agar 18.0g; dissolved in 1000mL distilled water; pH 7.0-7.5.

[0053] Qualitative test: Inoculate strain YM-1 onto organic phosphorus-free medium (containing lecithin), incubate at 27°C for 3 days, and observe whether a clear zone appears around the colony.

[0054] Quantitative testing: The prepared concentration was 1×10⁻⁶. 7 YM-1 spore suspensions at cfu / mL were inoculated at a rate of 1% into liquid culture media containing different NaCl concentration gradients (2.5%-15.0%) and different pH values ​​(7-12). The media were cultured at 27℃ and 200 r / min for 5 days with shaking. The organic phosphorus content in the supernatant was determined by the molybdenum blue colorimetric method.

[0055] Experimental data: Table 1: Soluble organic phosphorus content of strain YM-1 Note: Different letter symbols indicate significant differences in the mean between groups (p<0.05).

[0056] Experimental conclusion: A clear circle of transparency was observed in the qualitative experiment (see...). Figure 6 This indicates that strain YM-1 possesses the ability to solubilize organophosphates. Quantitative experiments show that strain YM-1 exhibits good organophosphate solubilization ability under conditions of NaCl concentrations of 2.5%–5.0% and pH values ​​of 7–9.

[0057] Test Example 3: Test of Inorganic Phosphorus Solving Ability of Strain YM-1 Experimental description: Culture medium: glucose 10.0g; ammonium sulfate 0.5g; yeast extract 0.5g; sodium chloride 0.3g; potassium chloride 0.3g; magnesium sulfate 0.3g; ferrous sulfate 0.03g; manganese sulfate 0.03g; tricalcium phosphate 5.0g; agar 18g; dissolved in 1000mL distilled water; pH 7.0-7.5.

[0058] Qualitative test: Inoculate strain YM-1 onto inorganic phosphate-free medium (containing tricalcium phosphate), incubate at 27°C for 3 days, and observe whether a clear zone appears around the colony.

[0059] Quantitative testing: The prepared concentration was 1×10⁻⁶. 7 YM-1 spore suspensions at cfu / mL were inoculated at a rate of 1% into inorganic phosphorus-dissolving liquid culture media containing different NaCl concentration gradients (2.5%-15.0%) and different pH values ​​(7-12). The media were cultured at 27℃ and 200 r / min for 5 days with shaking. The inorganic phosphorus content in the supernatant was determined by the molybdenum antimony colorimetric method.

[0060] Experimental data: Table 2: Soluble inorganic phosphorus content of strain YM-1 Note: Different letter symbols indicate significant differences in the mean between groups (p<0.05).

[0061] Experimental conclusion: A clear ring of light was observed in the qualitative experiment. Figure 7 This indicates that strain YM-1 possesses the ability to solubilize inorganic phosphorus. Quantitative experiments show that strain YM-1 exhibits good inorganic phosphorus solubilization ability under conditions of NaCl concentrations of 2.5%–5.0% and pH values ​​of 7–9.

[0062] Test Example 4: Degradation ability of strain YM-1 against pesticide MCPA Experimental instructions: The strain was inoculated into PDB medium and the seed culture was shaken to prepare a concentration of 1×10⁻⁶. 7 YM-1 spore suspensions at cfu / mL were inoculated at a rate of 1% into liquid culture media containing different NaCl concentration gradients (2.5%-15.0%) and different pH values ​​(7-12), and each medium contained 20 mg / L of the pesticide MCPA. After incubation at 27℃ and 200 rpm for 15 days, the residual MCPA in the supernatant was determined by high performance liquid chromatography.

[0063] Experimental data: The experimental results represent the residual concentration of MCPA, such as... Figure 8 As shown.

[0064] Experimental conclusions: The results show that strain YM-1 can maintain its ability to degrade MCPA under NaCl concentrations of 0%-7.5% and pH values ​​of 7-10. At pH 7-8, the degradation rates were 47.07% and 29.68%, respectively; at NaCl concentrations of 2.5% and 5.0%, the degradation rates were 43.32% and 36.02%, respectively.

[0065] Test Example 5: Analysis of the effect of strain YM-1 on maize seed germination under salt and alkali stress Experimental description: This test used the experimental setup of Example 1 and Comparative Example 1 to observe the cultured maize seeds, measure their sprout length, and perform statistical analysis.

[0066] Experimental data: as shown in Table 3 and Figure 9 As shown.

[0067] Table 3: Effects of strain YM-1 on maize seed germination under salt-alkali stress Note: (1) CK: water; MS: medium salt solution; YM-1: YM-1 spore suspension.

[0068] (2) Different letter labels indicate that there are significant differences in the mean between groups (p<0.05).

[0069] Experimental conclusion: Compared with Comparative Example 1, the average shoot length of maize inoculated with strain YM-1 in Example 1 (2.87 cm) was higher than that of the uninoculated stress group (0.52 cm), indicating that strain YM-1 can improve the salt tolerance of maize seeds and promote seed germination.

[0070] Test Example 6: Analysis of the growth-promoting effect of strain YM-1 on maize in saline-alkali land Experimental description: This test used the experimental setup of Example 2 and Comparative Example 2. After 20 days of cultivation, the plants were collected and their plant height and root length were measured for statistical analysis.

[0071] Experimental data: as shown in Table 4 and Figure 10 As shown.

[0072] Table 4: Results of the experiment on the relief of maize saline-alkali stress by strain YM-1 Note: (1) CK: water; MS: medium salt solution; YM-1: YM-1 spore suspension.

[0073] (2) Different letter labels indicate that there are significant differences in the mean between groups (p<0.05).

[0074] Experimental conclusion: Compared with Comparative Example 2, the average plant height and root length of maize inoculated with strain YM-1 in Example 2 increased by 10.49% and 17.01%, respectively, compared with the uninoculated stress group. Therefore, inoculation with YM-1 can effectively alleviate salt-alkali stress in maize and promote its growth.

[0075] Test Example 7: Analysis of the effect of strain YM-1 on phosphorus accumulation in maize Experimental description: This test used plant samples from Example 3 and Comparative Example 2. By detecting total phosphorus concentration and plant dry weight, the phosphorus accumulation in the aboveground and underground parts was calculated and compared.

[0076] Experimental data: Experimental results are as follows Figure 11 As shown.

[0077] Experimental conclusion: The results showed that, compared with the plants in Comparative Example 2, the average phosphorus accumulation in the aboveground parts of the plants inoculated with YM-1 in Example 3 increased by 34.43% (reaching 6.52 mg / plant), and the average phosphorus accumulation in the underground parts increased by 12.73% (reaching 0.62 mg / plant). This indicates that inoculation with YM-1 can promote phosphorus accumulation in maize.

Claims

1. A salt- and alkali-tolerant phosphorus-solubilizing and pesticide-reducing fungus YM-1, characterized in that, The salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus YM-1 is a purple-blue shaped fungus. Talaromyces purpureogenus The strain number is YM-1, and the preservation number is CGMCCNo.42313.

2. A culture, characterized in that, The culture is the substance obtained by culturing the purple-blue bacterium described in claim 1 in a bacterial culture medium.

3. A microbial agent, characterized in that, The bacterial agent contains the violet-producing bacterium as described in claim 1, the metabolites of the violet-producing bacterium as described in claim 1, and / or the culture as described in claim 2.

4. The microbial agent according to claim 3, characterized in that, The microbial agent is either a plant growth promoter or a pesticide residue degrading agent in the soil.

5. Application of a salt-tolerant, alkali-tolerant, phosphorus-solubilizing, and pesticide-reducing fungus YM-1 in maize cultivation, characterized in that... The application refers to the application of the purple-blue morphological fungus as described in claim 1, the culture as described in claim 2, or the microbial agent as described in claim 3 in agriculture.

6. The application of the salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus YM-1 according to claim 5 in maize cultivation, characterized in that, The application is used in the preparation of at least one of the following products: (1) Promotes plant growth; (2) Degrading organic phosphorus, inorganic phosphorus and / or MCPA pesticide residues in soil; (3) Alleviate salt stress and / or alkali stress in plants.

7. The application of the salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus YM-1 according to claim 6 in maize cultivation, characterized in that, The promotion of plant growth includes: promoting seed germination, increasing seedling emergence rate, increasing plant height, root length and / or biomass.

8. The application of the salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus YM-1 according to claim 6 in maize cultivation, characterized in that, The application was carried out under salt stress and / or alkali stress conditions.

9. The application of the salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus YM-1 according to claim 6 in maize cultivation, characterized in that, The application also includes promoting phosphorus accumulation in plants.

10. The application of the salt-tolerant, alkali-resistant, phosphorus-solubilizing, and pesticide-reducing fungus YM-1 according to claim 6 in maize cultivation, characterized in that, The plant is any one of the following: maize, maize, grasses, and monocotyledons.

Images