High-stability miniature seamless capsule as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of edible blasting beads, and particularly relates to a high-stability miniature seamless capsule as well as a preparation method and application thereof. The high-stability miniature seamless capsule comprises a core liquid and a wall material coating the core liquid, the wall material comprises the following raw materials: collagen, sugar / sugar alcohol compounds, alginate, glycerol and water; the core liquid comprises the following raw materials: an oily solvent and an antioxidant. The micro seamless capsule disclosed by the invention has the beneficial effects that the micro seamless capsule disclosed by the invention can tolerate high-temperature treatment of 80 DEG C, also can tolerate vacuum freeze drying, and can bear water-soluble and oil-soluble nutrients. When being applied to pet food, pet health care products, pet dried snacks and freeze-dried food, the nutrient processing performance and the product stability can be improved, meanwhile, the bad flavor of some nutrient substances (such as some vitamins, mineral substances and plant extracts) can be covered, and the palatability is improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of edible burst beads, and particularly relates to a high-stability micro seamless capsule and a preparation method and application thereof. BACKGROUND

[0002] The seamless capsule is a capsule dosage form formed by wrapping oil by a high-molecular hydrophilic colloid through 2 or 3 concentric devices, entering paraffin oil through interfacial tension, gravity and various forces. Compared with the soft capsule or hard capsule prepared by the traditional extrusion forming technology, the seamless capsule is once-formed by a dropping method and has no obvious joint or locking line. This unique manufacturing process brings many significant advantages to the field of nutrient embedding. First, it has excellent sealing and protection, can more effectively protect oxygen-sensitive (such as Omega-3, vitamin A, E, carotenoids), moisture-sensitive (such as probiotics, certain enzyme preparations) or light-sensitive nutrients, greatly delays the oxidation rancidity and degradation process, and prolongs the product shelf life. Secondly, the seamless capsule has higher bioavailability and realizes the precise delivery of nutrients, such as nutrients easily destroyed by gastric acid, such as probiotics, certain polypeptides and enzymes. At the same time, it can perfectly mask the bad taste of some nutrients, such as the fishy smell of fish oil and the metallic taste of iron agents, and improve the consumption experience. Finally, the product form and design of the seamless capsule have good flexibility, and can be designed into spherical, ellipsoidal, different sizes and colors to meet the diversified needs of consumers. Due to its many excellent properties, the seamless capsule has been applied to many fields such as medicines, functional foods, leisure foods, health products and pet foods.

[0003] The capsule shell of the seamless capsule is generally composed of gelatin, plant glue, sugar substances and plasticizers. The capsule shell with gelatin as the main supporting wall material will be melted at high temperature due to protein denaturation, and the water solubility of the prepared seamless capsule is poor, and the capsule shell is easy to stick together during high-temperature storage.

[0004] The prior art discloses a high-temperature-resistant improved sodium alginate film and a preparation method. The raw material of the high-temperature-resistant improved sodium alginate film includes sweet potato leaf extract and sodium alginate, the sodium alginate and Ca 2+The cross-linking can form a dense heat irreversible gel, improve the strength and elasticity of the film, and have high temperature resistance. The prior art also discloses a preparation method of a freeze-resistant core-bursting bead. The wall material of the bead uses sodium alginate, pectin and glycerol and an acidic substance. The calcium in the core layer is cross-linked with the sodium alginate to promote the formation of a gel film. At the same time, the amino and carboxyl groups in chitosan are cross-linked with the carboxyl groups in the alginate and the hydroxyl groups in the pectin, further improving the structural stability and compactness of the wall material and the freeze resistance of the wall material. However, the products prepared by the above two schemes are not seamless capsules. The calcium ion-containing core pellet is dropped into a solution containing sodium alginate to obtain the burst bead by the reverse solidification method, and the burst bead can only resist-18℃ conditions. The pre-freezing temperature of the vacuum freeze-drying is about-40℃, and the vacuum degree is 60-80 Pa. At present, there is no related process and preparation method of a burst bead that can resist the freeze vacuum drying process in the seamless capsule dosage form.

[0005] The seamless capsule prepared by using collagen, sugar compounds and sodium alginate as the composite wall material has good high temperature resistance and mechanical properties, and has good water solubility after drying, can quickly disintegrate in the oral cavity, and has better experience. The sugar compounds are rich in hydroxyl groups, which can form hydrogen bonds with the hydrophilic groups in gelatin, improve the toughness of the gelatin film, and enhance the gel strength. In addition to the plasticizing effect, these substances are more important because they have the function of "low-temperature protective agent". They can strongly bind with water molecules, reduce the free water content of the system, and thus inhibit the nucleation and growth of ice crystals. For example, trehalose is a very good low-temperature protective agent. It can form an amorphous glass body in a frozen state through glass transition, instead of sharp ice crystals, thereby protecting the structure of the film. At the same time, the collagen and sodium alginate composite film has a dense network structure after being solidified by calcium ions, which can improve the high temperature resistance of the film. Therefore, the addition of sugar compounds and sodium alginate to the collagen system can enhance the freeze resistance and high temperature resistance of the film.

[0006] Therefore, the micro seamless capsule prepared in the present application can resist 80℃ high temperature treatment and vacuum freeze drying process, and can be applied to pet freeze-dried and baked food. SUMMARY

[0007] The present application provides a micro seamless capsule which can resist high temperature and vacuum freezing conditions and can protect the quality of products with high stability, and a preparation method and application thereof.

[0008] The first aspect of the present application provides a micro seamless capsule with high stability, comprising a core liquid and a wall material covering the core liquid. The wall material comprises the following raw materials: collagen, sugar / sugar alcohol compounds, alginate, glycerol and water. The core liquid comprises the following raw materials: an oily solvent and an antioxidant.

[0009] According to some embodiments of the high-stability micro-seamless capsule, the particle size of the micro-seamless capsule is 1.5-3.0 mm.

[0010] According to some embodiments of the high-stability micro-seamless capsule, the mass ratio of the core liquid to the wall material is (65-90):(10-35).

[0011] According to some embodiments of the high-stability micro-seamless capsule, the mass ratio of collagen, sugar / sugar alcohol compounds, alginate, glycerol and water in the wall material is (5-25):(2-18):(0.1-4):(1.5-10):(65-85).

[0012] According to some embodiments of the high-stability micro-seamless capsule, the mass ratio of the oily solvent and the antioxidant in the core liquid is (99-99.9):(0.1-1).

[0013] According to some embodiments of the high-stability micro-seamless capsule, the collagen is derived from one or more of cowhide, cow bone, pigskin and fish skin.

[0014] According to some embodiments of the high-stability micro-seamless capsule, the sugar / sugar alcohol compounds include one or more of trehalose, glucose, maltose, isomaltose, isomaltitol, mannitol, xylitol, fructooligosaccharide, sorbitol and erythritol.

[0015] According to some embodiments of the high-stability micro-seamless capsule, the oily solvent includes one or more of peanut oil, soybean oil, rapeseed oil, olive oil, medium-chain triglyceride, sesame oil, fish oil and nannochloropsis oil.

[0016] According to some embodiments of the high-stability micro-seamless capsule, the antioxidant includes one or a combination of vitamin E oil and carnosic acid.

[0017] The second aspect of the present application provides a preparation method of the high-stability micro-seamless capsule of the first aspect of the present application, comprising the following steps: (1) mixing the oily solvent and the antioxidant to obtain a core liquid; (2) mixing collagen, sugar / sugar alcohol compounds, alginate, glycerol and water to obtain a wall material liquid; (3) preparing the core liquid and the wall material liquid into a capsule shape by the drop pill method, and performing cooling treatment to obtain the high-stability micro-seamless capsule.

[0018] According to some embodiments of the preparation method of the high-stability micro-seamless capsule, in step (1), the temperature of the mixing is 25-35℃, and the mixing time is 3-8 min.

[0019] According to some embodiments of the preparation method of the high-stability micro-seamless capsule, in step (2), the temperature of the mixing is 70-75℃, and the mixing time is 1-3 h.

[0020] According to some embodiments of the preparation method of the high-stability micro-seamless capsule, in step (3), the cooling treatment comprises a first cooling treatment and a second cooling treatment; preferably, the first cooling treatment comprises cooling treatment in a MCT first cooling liquid at 15-25℃ for 3-8 min; the second cooling treatment comprises cooling treatment in a second cooling liquid at 15-25℃ for 8-15 min, and the second cooling liquid comprises a mixture of calcium chloride with a mass concentration of 2% and calcium lactate with a mass concentration of 1%.

[0021] The third aspect of the present application provides an application of the high-stability micro-seamless capsule of the first aspect of the present application or the high-stability micro-seamless capsule obtained by the preparation method of the second aspect of the present application in pet food.

[0022] According to some embodiments of the application, the pet food comprises pet freeze-dried food or pet baked food.

[0023] The beneficial effects of the present application include that the micro-seamless capsule of the present application can withstand high temperature treatment of 80℃, can withstand vacuum freeze-drying, and can carry water-soluble and oil-soluble nutrients. When applied to pet food, pet health products, pet baked snacks and freeze-dried food, the processing performance and product stability of the nutrients can be improved, and the unpleasant flavor of certain nutrients (such as certain vitamins, minerals, and plant extracts) can be covered, and the palatability can be improved. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 A physical picture of the high-stability micro-seamless capsule prepared in Example 1 of the present application; Figure 2 A physical picture of the high-stability micro-seamless capsule prepared in Example 2 of the present application; Figure 3 A physical picture of the chicken freeze-dried snack prepared by the high-stability micro-seamless capsule of Example 2 of the present application; Figure 4 A physical picture of the chicken baked snack prepared by the high-stability micro-seamless capsule of Example 2 of the present application. DETAILED DESCRIPTION

[0025] Embodiments of the present application are described in detail below, examples of which are exemplary and intended to explain the present application, and cannot be understood as a limitation of the present application.

[0026] In the present application, the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the person skilled in the art can combine and combine the different embodiments or examples described in the present specification and the features of the different embodiments or examples without contradiction.

[0027] Embodiment 1 A method for preparing a high-stability micro-seamless capsule, comprising the following steps: (1) Wall material: prepare wall material raw materials bovine bone-derived collagen, erythritol, sodium alginate, glycerol and water in a mass ratio of 15:6:2:5.2:71.8; (2) Core material: prepare core material fish oil and vitamin E oil in a mass ratio of 99.5:0.5; (3) Add the wall material raw materials in step (1) to water (the mass ratio of the amount of water to the total amount of wall material raw materials is 71.8:28.2), stir uniformly at a temperature of 75°C, and then use ultrasonic waves to eliminate air bubbles to obtain a wall material liquid; (4) Mix the core material raw materials in step (2), use a shear machine to shear at a speed of 3 thousand revolutions / min for 5 min, and after the shearing is completed, use a vacuum drying box to remove air bubbles from the core liquid at 25°C and -0.8 bar for 5 min to obtain a core material; (5) The core material and the wall material pass through a gel pill machine to obtain a micro-seamless capsule resistant to high temperature and lyophilization process, the temperature of the wall material is controlled at 70°C, the temperature of the core material is controlled at 30°C, the wall material and the core material pass through a double-layer designed drop nozzle at the same time, then enter a first cooling liquid MCT at 20°C for cooling for 5 min, then enter a second cooling liquid (a mixed liquid of calcium chloride and calcium lactate, the mass concentration of calcium chloride and calcium lactate in the mixed liquid is 2% and 1% respectively) at 20°C for cooling for 10 min, and then are placed into a rotary drum dryer at 25°C and 30% humidity for drying for 1.5 h, and thus the micro-seamless capsule is obtained.

[0028] The actual picture of the high-stability micro-seamless capsule prepared in Embodiment 1 of the present application is shown in Figure 1 .

[0029] Embodiment 2 A preparation method of a high-stability micro-seamless capsule, comprising the following steps: (1) Wall material: prepare wall material raw materials bovine bone-derived collagen, trehalose, sodium alginate, glycerol and water in a mass ratio of 15:6:2:5.2:71.8; (2) Core material: prepare oily solvent and vitamin E oil in a mass ratio of 99.5:0.5; wherein the oily solvent is a mixture of medium-chain triglyceride: pseudo-micro green algae oil = 9:1; (3) Add the wall material raw materials in step (1) into water (the mass ratio of the amount of water to the total amount of wall material raw materials is 71.8:28.2), stir uniformly at a temperature of 70°C, and then use ultrasonic waves to eliminate air bubbles to obtain a wall material liquid; (4) Mix the core material raw materials in step (2), use a shear machine to shear at a speed of 3 thousand revolutions / min for 10 min, after the shearing is completed, use a vacuum drying box to remove air bubbles in the core liquid at a temperature of 25°C and a pressure of -0.8 bar for 5 min to obtain a core material; (5) Control the temperature of the wall material to be 70°C and the temperature of the core material to be 30°C, and then pass the core material and the wall material through a gel pill machine to obtain a micro-seamless capsule resistant to high temperature and lyophilization process, and then pass the wall material and the core material through a double-layer designed drop nozzle into 20°C first cooling liquid MCT to cool for 5 min, and then into 20°C second cooling liquid (a mixture of calcium chloride and calcium lactate, the mass concentration of calcium chloride and calcium lactate in the mixture is 2% and 1% respectively) to cool for 10 min, and then put into a rotary drum dryer to dry at a temperature of 25°C and a humidity of 30% for 1.5 h, and the high-stability micro-seamless capsule is obtained.

[0030] The actual picture of the high-stability micro-seamless capsule prepared in Example 2 is shown in Figure 2 .

[0031] Example 3 The difference between the preparation method of the high-stability micro-seamless capsule in Example 3 and that in Example 1 is that isomalt is used instead of erythritol in the preparation process of the high-stability micro-seamless capsule in Example 3.

[0032] Example 4 The difference between the preparation method of the high-stability micro-seamless capsule in Example 4 and that in Example 1 is that mannitol is used instead of erythritol in the preparation process of the high-stability micro-seamless capsule in Example 4.

[0033] Example 5 The difference between the preparation method of the high-stability micro-seamless capsule in Example 5 and that in Example 1 is that maltose is used instead of erythritol in the preparation process of the high-stability micro-seamless capsule in Example 5.

[0034] Comparative Example 1 The preparation method of the high-stability micro-seamless capsule of Comparative Example 1 is different from that of Example 1 only in that no raw material erythritol is added in the preparation process of the wall material of the high-stability micro-seamless capsule of Comparative Example 1.

[0035] Comparative Example 2 The preparation method of the high-stability micro-seamless capsule of Comparative Example 2 is different from that of Example 1 only in that no raw material sodium alginate is added in the preparation process of the wall material of the high-stability micro-seamless capsule of Comparative Example 2.

[0036] Comparative Example 3 The preparation method of the high-stability micro-seamless capsule of Comparative Example 3 is different from that of Example 1 only in that no raw materials erythritol and sodium alginate are added in the preparation process of the wall material of the high-stability micro-seamless capsule of Comparative Example 3.

[0037] Comparative Example 4 The preparation method of the high-stability micro-seamless capsule of Comparative Example 4 is different from that of Example 1 only in that the wall material of the high-stability micro-seamless capsule of Comparative Example 4 is not solidified by entering the second cooling liquid in the cooling process.

[0038] Comparative Example 5 The preparation method of the high-stability micro-seamless capsule of Comparative Example 5 is different from that of Example 1 only in that the amount and ratio of erythritol and sodium alginate used in the preparation process of the wall material of the high-stability micro-seamless capsule of Comparative Example 5 are different from those of Example 1.

[0039] The specific operation steps include: The wall material: prepare the wall material raw materials bovine bone-derived collagen, erythritol, sodium alginate, glycerol and water in a mass ratio of 15:4:2.5:5.2:73.3; the remaining operation steps are the same as those of Example 1.

[0040] Comparative Example 6 The preparation method of the high-stability micro-seamless capsule of Comparative Example 6 is different from that of Example 1 only in that the amount and ratio of erythritol and sodium alginate used in the preparation process of the wall material of the high-stability micro-seamless capsule of Comparative Example 6 are different from those of Example 1.

[0041] The specific operation steps include: The wall material: prepare the wall material raw materials bovine bone-derived collagen, erythritol, sodium alginate, glycerol and water in a mass ratio of 15:4:2.5:5.2:73.3; the remaining operation steps are the same as those of Example 1.

[0042] Comparative Example 7 The preparation method of the high-stability micro-seamless capsule of Comparative Example 7 is different from that of Example 1 only in that the ratio of erythritol and sodium alginate used in the preparation of the wall material of the high-stability micro-seamless capsule of Comparative Example 7 is different from that of Example 1.

[0043] The specific operation steps include: The wall material: the wall material raw materials, bovine bone-derived collagen, erythritol, sodium alginate, glycerol and water, are prepared in a mass ratio of 15:10:1:5.2:68.8; the remaining operation steps are the same as those of Example 1.

[0044] 1. High-temperature resistance performance research and vacuum freeze-drying performance research of the micro-seamless capsules of Examples 1-5 and Comparative Examples 1-7 of the present application High-temperature resistance test: the micro-seamless capsules of Examples 1-5 and Comparative Examples 1-7 of the present application were subjected to oven heating high-temperature resistance test, and the appearance damage of the seamless capsules was observed after heating at 50℃ and 80℃ for 2 h, respectively. Table 1 shows the appearance change after high-temperature test.

[0045] Vacuum freeze-drying resistance test: first, the micro-seamless capsules of Examples 1-5 and Comparative Examples 1-7 of the present application were pre-cooled in a freezer at -40℃ for 2 h, and then were placed in a vacuum freeze-drying machine, and the heating temperature was controlled at 40℃, and the vacuum degree was 60 Pa, and the seamless capsules were dried for 25 h, and then the appearance damage of the seamless capsules was observed. Table 1 shows the appearance change after oven heating and vacuum freeze-drying test.

[0046] Table 1: High-temperature and vacuum freeze-drying resistance test of micro-seamless capsules As can be seen from Table 1, the seamless capsules made of the wall material composed of collagen, sodium alginate and sugar / sugar alcohol compounds can withstand heating at 80℃ for 2 h after the first and second cooling liquids, and have excellent high-temperature resistance performance. However, the seamless capsules made of the wall material composed of collagen (Comparative Example 3), collagen and sugar compounds (Comparative Example 2), and collagen and sodium alginate and sugar compounds without calcium ion solidification (Comparative Example 4) will be broken after heating at more than 50℃, and cannot withstand high-temperature heating.

[0047] Collagen and sugar / sugar alcohol complex (comparative example 2) can partially tolerate vacuum freeze-drying conditions, and the complex of collagen, sugar / sugar alcohol and sodium alginate (examples 1-5) further enhances the tolerance to vacuum freeze-drying, while the collagen wall material alone (comparative example 3) cannot tolerate high temperature and vacuum freeze-drying conditions. At the same time, it is found that when the ratio of erythritol:sodium alginate is (4-8):(1.5-2.5), the prepared composite wall material has excellent tolerance performance, and when the amount of erythritol added is more than 8% and the amount of sodium alginate added is less than 1.5%, the structure of the composite wall material becomes dense, the rubber becomes brittle, and cannot tolerate heat processing and freeze-drying. It is found that the above-mentioned, the complex of collagen, sugar and sodium alginate within a reasonable range, and the wall material after calcium ion cross-linking can withstand high temperature and vacuum freeze-drying conditions, and can be applied to pet baked and freeze-dried food, health products and snacks.

[0048] 2. Preparation of freeze-dried snacks containing the micro-seamless capsules of example 2 of the present application The micro-seamless capsules of example 2 of the present application are applied to cat hair removal freeze-dried snacks, and the freeze-dried snacks are composed of fresh chicken breast, chicken liver, cellulose, psyllium husk, fructo-oligosaccharide, water, and micro-seamless capsules, and the preparation method is as follows: (1) thaw the chicken breast and chicken liver at room temperature, cut the meat into small pieces and put them in a chopper for processing; (2) mix the fresh chicken breast, chicken liver, cellulose, psyllium husk, fructo-oligosaccharide and water according to the mass ratio of 64:4:2:3:0.5:26.5, mix them evenly with a chopper, and finally add 3% of the total mass of the mixed material to the micro-seamless capsules and mix them evenly by hand; (3) pour the mixed material into a silica gel mold, pre-freeze it in a freezer at-40℃ for 2h, then put it into a vacuum freeze-drying machine for drying treatment, and then put it into a vacuum freeze-drying machine at a temperature below 40℃ and a vacuum degree of 60pa for 30h to obtain the freeze-dried product as shown in Figure 3 .

[0049] As can be seen from Figure 3 , the micro-seamless capsules added to the freeze-dried snacks do not break and can withstand the vacuum freeze-drying process. The micro-wire capsules contain nannochloropsis oil, which contains rich EPA and other omega-3 fatty acids that can beautify the skin and improve the application rate of nutrients, preventing loss during processing.

[0050] 3. Preparation of pet chicken baked snacks containing the micro-seamless capsules of example 2 of the present application The micro-seamless capsules prepared in example 2 are applied to pet chicken baked snacks, and the baked snacks are composed of fresh chicken breast, rosemary extract and micro-seamless capsules, and the preparation method is as follows: (1) The chicken breast meat is thawed at room temperature, and the meat is cut into small pieces and put into a chopper for processing; (2) The fresh chicken breast meat and rosemary extract are mixed according to the mass ratio of 99.8:0.2, and a chopper is used for mixing to obtain a mixed material. Finally, 3% of the total mass of the mixed material is added to the micro-seamless capsules, and the mixed material is manually mixed uniformly; (3) An appropriate amount of mixed material liquid is poured into a stainless steel tray, and placed in an oven at 70°C for 4 h to obtain the dried snack as shown in Figure 4 .

[0051] As can be seen from Figure 4 , the micro-seamless capsules added to the chicken dried snack maintain the integrity of the appearance, have no cracking, and can withstand high-temperature drying processes.

[0052] Although the above embodiments have been shown and described, it can be understood that the above embodiments are exemplary and cannot be understood as limiting the application, and the changes, modifications, replacements and variations of the above embodiments made by those of ordinary skill in the art are within the protection scope of the application.

Claims

1. A high-stability micro-seamless capsule characterized in that, The core liquid and the wall material surrounding the core liquid; The wall material comprises the following raw materials: collagen, sugar / sugar alcohol compound, alginate, glycerol and water; The core liquid comprises the following raw materials: oily solvent and antioxidant.

2. The high-stability microseamless capsule according to claim 1, characterized in that, The particle size of the micro-seamless capsule is 1.5-3.0mm; And / or, the mass ratio of the core liquid to the wall material is (65-90):(10-35).

3. The high-stable micro-seamless capsule according to claim 1, wherein, The mass ratio of collagen, sugar / sugar alcohol compound, alginate, glycerol and water in the wall material is (5-25):(2-18):(0.1-4):(1.5-10):(65-85); And / or, the mass ratio of oily solvent to antioxidant in the core liquid is (99-99.9):(0.1-1).

4. The high-stability microseamless capsule according to claim 1, characterized in that, The collagen is derived from one or more of the following: cowhide, cow bone, pigskin and fish skin; And / or, the sugar / sugar alcohol compound comprises one or more of the following: trehalose, glucose, maltose, isomaltose, isomaltitol, mannitol, xylitol, fructooligosaccharide, sorbitol and erythritol.

5. The high-stable micro-seamless capsule according to claim 1, wherein, The oily solvent comprises one or more of the following: peanut oil, soybean oil, rapeseed oil, olive oil, medium-chain triglyceride, sesame oil, fish oil and pseudomicroalgae oil; And / or, the antioxidant comprises one or a combination of the following: vitamin E oil and carnosic acid.

6. Process for the preparation of microcapsules of high stability according to any one of claims 1 to 5, characterized in that, The method comprises the following steps: (1) mixing oily solvent and antioxidant to obtain core liquid; (2) mixing collagen, sugar / sugar alcohol compound, alginate, glycerol and water to obtain wall material liquid; (3) preparing the core liquid and the wall material liquid into capsule shape by drop pill method, and performing cooling treatment to obtain the micro-seamless capsule with high stability.

7. The process for the preparation of microcapsules with high stability according to claim 6, characterized in that, In step (1), the temperature of the mixing is 25-35℃, and the mixing time is 3-8min.

8. The process for the preparation of microcapsules with high stability according to claim 6, characterized in that, In step (2), the temperature of the mixing is 70-75℃, and the mixing time is 1-3h; In step (3), the cooling treatment comprises first cooling treatment and second cooling treatment; preferably, the first cooling treatment comprises cooling treatment in 15-25℃ MCT first cooling liquid for 3-8min; the second cooling treatment comprises cooling treatment in 15-25℃ second cooling liquid for 8-15min, and the second cooling liquid comprises a mixture of 2% calcium chloride and 1% calcium lactate.

9. Use of the micro-seamless capsule with high stability according to any one of claims 1-5 or obtained by the preparation method according to any one of claims 6-8 in pet food.

10. Use according to claim 9, characterized in that, The pet food comprises pet freeze-dried food or pet baked food.

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