Nest type full-automatic immunofluorescence analyzer
By designing the card pushing, incubation, and detection structure of the nested fully automated immunofluorescence analyzer, the problems of low space utilization and high failure rate in existing technologies have been solved, achieving efficient multi-channel detection and incubation while reducing costs.
Patent Information
- Application Number
- CN202512027573.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-30
- Publication Date
- 2026-02-17
AI Technical Summary
Existing fully automated immunofluorescence analyzers have low space utilization, a limited number of channels, complex structure, high cost, high failure rate, and are prone to malfunctions during incubation.
It adopts a nested structure design, including a card pushing structure, an incubation structure, and a detection structure. The card pushing structure continuously pushes out detection cards, the incubation structure incubates multiple cards at the same time, and the detection structure scans and discharges the cards.
It improves detection efficiency, reduces failure rate and cost, enables simultaneous multi-channel detection and incubation, and enhances space utilization.
Smart Images
Figure CN121540897A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of medical testing equipment technology, specifically a nested fully automated immunofluorescence analyzer. Background Technology
[0002] Immunofluorescence assay is an important in vitro diagnostic method that combines the high specificity of antigen-antibody reactions with the high sensitivity of fluorescent labeling. This technique utilizes the specific binding of fluorescently labeled antibodies to target analytes (antigens or antibodies) in a sample. After excitation with light of a specific wavelength, the fluorescence signal is detected, enabling qualitative and quantitative analysis of the target analyte. It is widely used in clinical testing for infectious diseases, autoimmune diseases, and tumor markers. The immunofluorescence assay process includes sample pretreatment (dilution and reagent mixing), sample addition, incubation, and testing. Fully automated products also require discarding the completed test strip.
[0003] Currently, most fully automated immunofluorescence analyzers use a disk-type structure, where test cards awaiting incubation are placed within a circular disk. This layout has low space utilization, resulting in a limited number of detection channels, typically no more than 64. Increasing the number of channels requires either increasing the radius of the reagent disk or increasing the number of reagent disks, both of which tend to lead to structural complexity, high cost, and high failure rates. Furthermore, because the reagent disk frequently moves in a circular motion, a heating film needs to be added to the reagent disk to achieve the incubation function, powered by brushes, which further increases the failure rate. Summary of the Invention
[0004] To address the problems in the prior art, the present invention provides a nested fully automated immunofluorescence analyzer.
[0005] The technical solution adopted by the present invention to solve its technical problem is: a nested fully automated immunofluorescence analyzer, including a frame, a pipetting pump mechanism installed on the top side of the frame, a push-card structure provided on the inner side of the frame, a storage structure connected to the push-card structure, an incubation structure provided on the side of the push-card structure, and a detection structure connected to the end of the incubation structure.
[0006] Specifically, the card-pushing structure includes a first guide rail, two first guide rails are installed parallel to each other on the bottom side of the frame, a second guide rail is slidably connected to the first guide rail, a first card-pushing motor is installed on the first guide rail, a second card-pushing motor is installed on the second guide rail, a first pushing block is slidably connected to the second guide rail, a support plate is fixedly connected to the frame, and a storage box is installed on the support plate.
[0007] Specifically, the storage structure includes card cartridges, and multiple card cartridges are slidably connected to the inner side of the storage box. Multiple test cards are provided on the inner side of each card cartridge, and multiple card dispensing slots are provided on the side of the storage box corresponding to the card cartridges.
[0008] Specifically, a counterweight plate is slidably connected to the inner side of the cartridge, and the counterweight plate abuts against the test card. An inclined clamp rod is fixedly connected to the end of the counterweight plate, and the inclined clamp rod has a "T" shaped structure.
[0009] Specifically, both sides of the inclined clamp are inclined structures. A limiting strip is slidably connected to both sides of the inclined clamp on the inner side of the storage box. The side of the inclined clamp is slidably connected to the limiting strip. A second spring is fixedly connected between the limiting strip and the storage box. A release groove is provided on the bottom side of the limiting strip.
[0010] Specifically, the incubation structure includes a third guide rail, which is mounted on the frame. A second push block is slidably connected to the third guide rail, and a card-moving motor is mounted on the side of the third guide rail.
[0011] Specifically, an incubation plate is installed on the bottom side of the frame, the second pusher is slidably connected to the incubation plate, and the surface of the incubation plate is flush with the first pusher.
[0012] Specifically, the detection structure includes a scanning module, which is slidably connected to the side of the frame. A detection motor is installed on the side of the frame, and a guide slot is provided on the side of the incubation plate. The guide slot is obliquely arranged.
[0013] Specifically, a mounting block is vertically fixedly connected to the end of the scanning module, a third push block is rotatably connected to the bottom end of the mounting block, and a torsion spring is fixedly connected between the third push block and the mounting block.
[0014] Specifically, a rotating block is fixedly connected to the side of the third push block, the side of the rotating block abuts against the mounting block, an inclined block is slidably connected to the side of the rotating block, a first spring is fixedly connected between the inclined block and the rotating block, the bottom side of the inclined block is an inclined structure, and an abutting rod is fixedly connected to the end of the bearing plate, the abutting rod being correspondingly arranged with the inclined block.
[0015] The beneficial effects of this invention are: (1) The nested fully automated immunofluorescence analyzer of the present invention has a card pusher structure on the inner side of the frame. The card pusher structure is connected to a storage structure. The card pusher structure can continuously push out the test cards stored in the box for detection. The storage structure can facilitate the storage and retrieval of the test cards.
[0016] (2) The nested fully automated immunofluorescence analyzer of the present invention has an incubation structure on the side of the push card structure, through which multiple test cards can be transported and incubated simultaneously.
[0017] (3) The nested fully automated immunofluorescence analyzer of the present invention has a detection structure connected to the end of the incubation structure. The detection structure can scan and detect the test card, and at the same time discharge the test card after the detection is completed. Attached Figure Description
[0018] The present invention will be further described below with reference to the accompanying drawings and embodiments.
[0019] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the connection structure between the frame and the first guide rail of the present invention; Figure 3 This is a schematic diagram of the connection structure between the third guide rail and the incubation plate of the present invention; Figure 4 for Figure 3 The diagram shows an enlarged view of part A. Figure 5 This is a schematic diagram of the connection structure between the incubation plate and the second pusher block of the present invention; Figure 6 This is a schematic diagram of the connection structure between the mounting block and the third push block of the present invention; Figure 7 This is a schematic diagram of the connection structure between the storage box and the card holder of the present invention; Figure 8 This is a schematic diagram of the connection structure between the cartridge and the counterweight plate of the present invention; Figure 9 for Figure 8 The diagram shows an enlarged view of part B.
[0020] In the diagram: 1. Frame; 2. Pipette pump mechanism; 3. Card pusher structure; 301. Support plate; 302. Storage box; 303. First pusher block; 304. First guide rail; 305. First card pusher motor; 306. Second guide rail; 307. Second card pusher motor; 4. Incubation structure; 401. Incubation plate; 402. Third guide rail; 403. Card transfer motor; 404. Second pusher block; 5. Detection structure; 501. Detection motor; 502. Card guide slot; 503. Scanning module; 504. Contact rod; 505. Mounting block; 506. Rotating block; 507. Inclined block; 508. First spring; 509. Third push block; 510. Torsion spring; 6. Storage structure; 601. Card outlet; 602. Card holder; 603. Counterweight plate; 604. Inclined card rod; 605. Test card; 606. Limiting strip; 607. Release groove; 608. Second spring. Detailed Implementation
[0021] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.
[0022] like Figure 1 , Figure 3 , Figure 7 As shown, the nested fully automated immunofluorescence analyzer of the present invention includes a frame 1, a pipetting pump mechanism 2 installed on the top side of the frame 1, a push-card structure 3 provided on the inner side of the frame 1, a storage structure 6 connected to the push-card structure 3, an incubation structure 4 provided on the side of the push-card structure 3, and a detection structure 5 connected to the end of the incubation structure 4.
[0023] Specifically, such as Figure 1 , Figure 2 , Figure 7 , Figure 8 , Figure 9 As shown, the card-pushing structure 3 includes a first guide rail 304. Two first guide rails 304 are installed parallel to each other on the bottom side of the frame 1. A second guide rail 306 is vertically slidably connected to the first guide rail 304. A first card-pushing motor 305 is installed on the first guide rail 304, and a second card-pushing motor 307 is installed on the second guide rail 306. A first push block 303 is slidably connected to the second guide rail 306. A support plate 301 is fixedly connected to the frame 1, and a storage box 302 is installed on the support plate 301. The card-pushing structure 3 can push the test cards 6 stored in the box. 05. Continuous ejection for testing improves testing efficiency. Specifically: First, the test card is stored in the storage box 302. A first pusher 303 is located on the bottom side of the support plate 301. The first pusher 303 is driven by the second pusher motor 307 via a belt and slides along the second guide rail 306. Furthermore, the second guide rail 306 is driven by the first pusher motor 305 via a belt and slides along the first guide rail 304. This allows the first pusher 303 to continuously eject the test cards stored in the storage box 302 from all directions, saving space and improving testing efficiency.
[0024] Specifically, such as Figure 1 , Figure 7 , Figure 8 , Figure 9As shown, the storage structure 6 includes a card holder 602. Multiple card holders 602 are slidably connected to the inner side of the storage box 302. Multiple test cards 605 are provided inside the card holders 602. Multiple card dispensing slots 601 are opened on the side of the storage box 302 corresponding to the card holders 602. A counterweight plate 603 is slidably connected to the inner side of the card holders 602. The counterweight plate 603 abuts against the test cards 605. A slanted locking rod 604 is fixedly connected to the end of the counterweight plate 603. The inclined locking rod 604 has a "T"-shaped structure, with both sides of the inclined locking rod 604 having an inclined structure. A limiting strip 606 is slidably connected to both sides of the inclined locking rod 604 on the inner side of the storage box 302. The side of the inclined locking rod 604 is slidably connected to the limiting strip 606. A second spring 608 is fixedly connected between the limiting strip 606 and the storage box 302. A release groove 607 is provided on the bottom side of the limiting strip 606. The storage structure 6 allows for convenient... The storage and retrieval of the test cards are performed as follows: Multiple card holders 602 are provided inside the storage box 302. Test cards are stacked inside the card holders 602. A counterweight plate 603 is located at the top of each card holder 602. When a card holder 602 is full of test cards and inverted inside the storage box 302, the counterweight plate applies pressure to the test cards. When the first pusher 303 pushes the test cards out of the card outlet 601 from the bottom of the storage box 302, the test cards in the card holders 602 will automatically fall out. To facilitate the subsequent ejection of test cards, when all test cards in the cartridge 602 are ejected, the inclined lever 604 at the end of the counterweight plate 603 will fall to the release groove 607 on the side of the limit bar 606. At this time, the user can take out the corresponding hole cartridge 602 from the storage box 302 from the side and reload the test cards. When the cartridge 602 is placed into the storage box 302, the inclined lever 604 will push the limit bars 606 on both sides to the sides through the inclined surface, which facilitates the installation of the cartridge 602.
[0025] Specifically, such as Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5As shown, the incubation structure 4 includes a third guide rail 402, which is mounted on the frame 1. A second pusher block 404 is slidably connected to the third guide rail 402. A card-moving motor 403 is mounted on the side of the third guide rail 402. An incubation plate 401 is mounted on the bottom side of the frame 1. The second pusher block 404 is slidably connected to the incubation plate 401, and the surface of the incubation plate 401 is flush with the first pusher block 303. Multiple detection cards can be processed through the incubation structure 4. Simultaneous delivery and incubation are performed: after the test card is pushed from the storage box 302 to the incubation plate 401 on the side by the first pusher 303, the card transfer motor 403 can drive the second pusher 404 to move in the middle of the incubation plate 401 via a belt. A three-axis moving pipette pump mechanism 2 is located on the top side of the frame 1 for functions such as adding samples and reagents. The module uses a pipette tip for liquid aspiration, effectively reducing cross-contamination and avoiding the increased cost and failure rate caused by liquid circuit design. The corresponding test card is pushed towards the end of the incubation plate 401 by the second pusher 404. After the test card has completed the addition of samples and reagents, the incubation process can be carried out on the incubation plate 401. Since the incubation plate 401 has a certain length, it can accommodate multiple test cards for simultaneous incubation, ensuring that multiple sets of tests are carried out synchronously.
[0026] Specifically, such as Figure 1 , Figure 3 , Figure 4 , Figure 5 , Figure 6As shown, the detection structure 5 includes a scanning module 503. The scanning module 503 is slidably connected to the side of the frame 1. A detection motor 501 is mounted on the side of the frame 1. A guide slot 502 is provided on the side of the incubation plate 401. The guide slot 502 is obliquely arranged. A mounting block 505 is vertically fixedly connected to the end of the scanning module 503. A third push block 509 is rotatably connected to the bottom end of the mounting block 505. A torsion spring 510 is fixedly connected between the third push block 509 and the mounting block 505. A rotating block 506 is fixedly connected to the side of the third push block 509. The side of the rotating block 506 abuts against the mounting block 505. The rotating block 506 has a slidably connected inclined block 507 on its side. A first spring 508 is fixedly connected between the inclined block 507 and the rotating block 506. The bottom side of the inclined block 507 is an inclined structure. An abutment rod 504 is fixedly connected to the end of the bearing plate 301. The abutment rod 504 is correspondingly arranged with the inclined block 507. The detection structure 5 can scan and detect the detection card, and at the same time, the detection card that has completed the detection is discharged. That is, after the detection card completes the incubation process on the incubation plate 401, the detection card is pushed to the end of the incubation plate 401 by the second pusher 404. A scanning module 503 is provided on the side of the end of the incubation plate 401. The scanning module 503 can be driven by the detection motor 501 via a screw. A mounting block 505 is installed at the end of the scanning module 503. When the scanning module 503 moves towards the incubation plate 401 under the drive of the detection motor 501, the rotating block 506 and the inclined block 507 on the side of the mounting block 505 will abut against the contact rod 504, thereby pushing the rotating block 506 and the connected third push block 509 to rotate. At this time, the third push block 509 rotates to a horizontal state, preventing it from contacting the detection card. Simultaneously, as the scanning module 503 moves... During the process, the scanning module 503 can scan and detect the sample on the detection card. After the scan is completed, the detection motor 501 drives the scanning module 503 to return to its original position. At this time, due to the inclined structure of the inclined block 507, when the inclined block 507 comes into contact with the contact rod 504, the inclined block 507 will retract to the inside of the rotating block 506. At this time, the third push block 509 will be in the extended state, and then the detection card that has completed the detection will be pushed to one side through the third push block 509. Finally, the detection card will be discharged through the guide card slot 502, so that the detection and discharge process of the sample can be carried out stably.
[0027] In use, the present invention first stores the test card in the storage box 302. A first pusher 303 is provided on the bottom side of the support plate 301. The first pusher 303 is driven by a second pusher motor 307 via a belt and slides along a second guide rail 306. The second guide rail 306 is further driven by a first pusher motor 305 via a belt and slides along a first guide rail 304. This allows the first pusher 303 to continuously push out the test card stored in the storage box 302 from all directions, saving space and improving testing efficiency. Multiple card holders 602 are provided inside the storage box 302. The test cards are stacked inside the card holders 602. A counterweight plate 603 is provided on the top side of the card holder 602. When the card holder 602 is full of test cards and is inverted in the storage box 302, the counterweight plate will apply a certain pressure to the test cards. When the first pusher 303 pushes the test card out of the card outlet 601 from the bottom side of the storage box 302, the test card in the card holder 602... The card will automatically drop down to facilitate the ejection of subsequent test cards. When all test cards in the card magazine 602 have been ejected, the inclined lever 604 at the end of the counterweight plate 603 will fall to the release groove 607 on the side of the limiting strip 606. At this time, the user can remove the corresponding card magazine 602 from the storage box 302 from the side and reload the test cards. When the card magazine 602 is placed into the storage box 302, the inclined lever 604 will push the limiting strips 606 on both sides to the sides through the incline, facilitating the ejection of the test cards. The installation of the cartridge 602 involves pushing the test card from the storage box 302 onto the incubation plate 401 on the side via the first pusher 303. The card transfer motor 403 can then drive the second pusher 404 to move in the middle of the incubation plate 401 via a belt. A three-axis moving pipette pump mechanism 2 is located on the top side of the frame 1 for functions such as adding samples and reagents. The module uses a pipette tip to aspirate liquid, effectively reducing cross-contamination and avoiding increased costs and failure rates caused by liquid circuit design. The corresponding test card is pushed towards the end of the incubation plate 401 by the second pusher 404. After the test card completes the addition of sample and reagent, the incubation process can be carried out on the incubation plate 401. Since the incubation plate 401 has a certain length, it can accommodate multiple test cards for incubation at the same time, ensuring that multiple sets of tests are carried out synchronously. After the test card completes the incubation process on the incubation plate 401, the test card is pushed to the end of the incubation plate 401 by the second pusher 404. A scanning module 503 is provided on the side of the end of the incubation plate 401.The scanning module 503 can be driven by the detection motor 501 via a screw. A mounting block 505 is installed at the end of the scanning module 503. When the scanning module 503 moves towards the incubation plate 401 under the drive of the detection motor 501, the rotating block 506 and the inclined block 507 on the side of the mounting block 505 will abut against the contact rod 504, thereby pushing the rotating block 506 and the connected third push block 509 to rotate. At this time, the third push block 509 rotates to a horizontal state, preventing it from contacting the detection card. Simultaneously, as the scanning module 503 moves... During the process, the scanning module 503 can scan and detect the sample on the detection card. After the scan is completed, the detection motor 501 drives the scanning module 503 to return to its original position. At this time, due to the inclined structure of the inclined block 507, when the inclined block 507 comes into contact with the contact rod 504, the inclined block 507 will retract to the inside of the rotating block 506. At this time, the third push block 509 will be in the extended state, and then the detection card that has completed the detection will be pushed to one side through the third push block 509. Finally, the detection card will be discharged through the guide card slot 502, so that the detection and discharge process of the sample can be carried out stably.
[0028] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.
[0029] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.
Claims
1. A nested fully automated immunofluorescence analyzer characterized in that: Including frame (1), the top side of frame (1) is provided with pipette pump mechanism (2), the inner side of frame (1) is provided with push card structure (3), push card structure (3) is connected with storage structure (6); The push card structure (3) includes a first guide rail (304), and the bottom side of the frame (1) is provided with two first guide rails (304) in parallel, and a second guide rail (306) is vertically and slidably connected to the first guide rail (304), a first push card motor (305) is installed on the first guide rail (304), a second push card motor (307) is installed on the second guide rail (306), and a first push block (303) is slidably connected to the second guide rail (306), and a bearing plate (301) is fixedly connected to the frame (1), and a storage box (302) is installed on the bearing plate (301).
2. The nested fully automated immunofluorescence analyzer according to claim 1, characterized in that: The storage structure (6) includes a card box (602), and a plurality of card boxes (602) are slidably connected to the inner side of the storage box (302), a plurality of test cards (605) are arranged on the inner side of the card box (602), and a plurality of card outlets (601) are formed in the side of the storage box (302) corresponding to the card box (602).
3. The nested fully automated immunofluorescence analyzer according to claim 2, characterized in that: The inner side of the card box (602) is slidably connected with a counterweight plate (603), the counterweight plate (603) abuts against the test card (605), and the end of the counterweight plate (603) is fixedly connected with an inclined clamping rod (604), and the inclined clamping rod (604) is in a "T" type structure.
4. The nested automatic immunofluorescence analyzer according to claim 3, characterized in that: The two sides of the inclined clamping rod (604) are inclined surfaces, and one limiting strip (606) is slidably connected to the inner side of the storage box (302) on the two sides of the inclined clamping rod (604), the side of the inclined clamping rod (604) is slidably connected with the limiting strip (606), and the limiting strip (606) and the storage box (302) are fixedly connected with a second spring (608), and the bottom side of the limiting strip (606) is provided with a release groove (607).
5. The nested fully automated immunofluorescence analyzer according to claim 2, characterized in that: The side of the push card structure (3) is provided with an incubation structure (4), the incubation structure (4) includes a third guide rail (402), the third guide rail (402) is installed on the frame (1), the second push block (404) is slidably connected to the third guide rail (402), and the third guide rail (402) is installed with a card moving motor (403).
6. The nested automatic immunofluorescence analyzer according to claim 5, characterized in that: The bottom side of the frame (1) is provided with an incubation plate (401), the second push block (404) and the incubation plate (401) are slidably connected, and the surface of the incubation plate (401) is aligned with the first push block (303).
7. The nested automatic immunofluorescence analyzer according to claim 6, characterized in that: The end of the incubation structure (4) is connected with a detection structure (5), the detection structure (5) includes a scanning module (503), the scanning module (503) is slidably connected to the side of the frame (1), the detection motor (501) is installed on the side of the frame (1), the side of the incubation plate (401) is provided with a card guide groove (502), and the card guide groove (502) is arranged obliquely.
8. The nested automated immunofluorescence analyzer according to claim 7, characterized in that: The end of the scanning module (503) is vertically fixedly connected with a mounting block (505), the bottom end of the mounting block (505) is rotatably connected with a third push block (509), and the third push block (509) and the mounting block (505) are fixedly connected with a torsion spring (510).
9. The nested automated immunofluorescence analyzer according to claim 8, characterized in that: The side surface of the third push block (509) is fixedly connected with a rotating block (506), the side surface of the rotating block (506) abuts against the mounting block (505), the side surface of the rotating block (506) is slidably connected with an inclined block (507), the inclined block (507) and the rotating block (506) are fixedly connected with a first spring (508), the bottom side of the inclined block (507) is of an inclined surface structure, the end of the bearing plate (301) is fixedly connected with an abutting rod (504), and the abutting rod (504) is correspondingly arranged with the inclined block (507).