Synthesis method of copper peptide

Abstract

The invention relates to a synthesis method of copper peptide, which comprises the following steps: keeping the temperature in a reaction kettle at 0-5 DEG C, and dissolving 100g of glycyl-histidyl-lysine hydrochloride in 0.1-2L of water to obtain a reaction solution; adjusting the pH value of the reaction liquid to 6.0-10.0 by using an alkaline material; and adding 0.9-3 equivalent weight of copper salt, adjusting the rotating speed to be 40-120 revolutions per minute, stirring until the copper salt is dissolved, maintaining the temperature to be 5-40 DEG C, and crystallizing for 12-36 hours to obtain the synthesized blue copper peptide crystal. The synthesis method of the copper peptide is simple to operate, mild in condition and suitable for industrial production; the yield is high and generally reaches more than 80%; the product quality is stable, and the moisture content is controllable; the crystal is good in morphology and has certain luster; the dissolvability is good, and subsequent application is facilitated.

Description

Technical Field

[0001] This invention relates to the field of copper peptide synthesis technology, and in particular to a method for synthesizing copper peptides. Background Technology

[0002] Glycyl-L-histyl-L-lysine (GHK) is a tripeptide found in human plasma. It was isolated from human plasma albumin by Pickart

[38] in 1973. GHK has a high affinity for Cu2+ ions and can chelate with Cu2+ to form GHK-Cu. As a complexing agent, GHK can also accelerate the migration of copper ions through the membrane, which is beneficial to the skin absorption of copper ions.

[0003] Copper ions promote endothelial cell proliferation in vitro in a dose-dependent manner. Copper ions also promote wound healing by upregulating the expression of vascular endothelial growth factor (VEGF). Furthermore, copper ions possess antibacterial properties. Direct and prolonged contact of copper with the skin may lead to electrochemical reactions that release copper ions, which can diffuse through the skin. By monitoring inflammatory responses, such as cytokine secretion and receptor activation, various biomarkers can provide an indication that copper can be safely and effectively transported through the skin to exert its beneficial effects. When GHK is conjugated with copper, the peptide quenches the redox activity of copper, promoting its non-toxic delivery into cells. GHK is commonly used in cosmetics and possesses properties such as stimulating collagen synthesis, chemotaxis, and antipruritic effects. Studies have shown that GHK itself is non-toxic and does not cause changes in irritation-related biomarkers, which further facilitates its complexation with copper.

[0004] Tripeptide-1 copper (GHK-Cu) is a small-molecule human copper peptide conjugate naturally found in human plasma, urine, saliva, and cerebrospinal fluid. It is composed of the tripeptide GlycyI-L-histidyI-L-lysine and its high-affinity divalent copper ions. Initially discovered by Pickart et al., it was isolated from human plasma and described as a growth factor for various differentiated cells and a regulator of the extracellular matrix. GHK plasma concentration is significantly correlated with the regenerative capacity of organisms; studies have shown that the plasma concentration of GHK-Cu in a 20-year-old is 2.5 times that in a 60-year-old. GHK also plays a powerful role in the repair of many diseases, promoting wound healing such as in diabetes and burns; combating acute lung injury; anti-anxiety effects; anti-infection effects; anti-pain and anti-inflammatory effects; promoting stem cell repair; inflammatory bowel disease; promoting liver repair; preventing neurodegenerative diseases; and promoting hair follicle repair, thereby promoting hair growth. In the skin, tripeptide-1 copper can tighten the skin, delay aging, repair skin barrier proteins, reduce fine lines and wrinkle depth, smooth the skin, reduce photodamage and pigmentation; it can also promote the proliferation of hair follicles, increase the size of hair follicles, and promote hair growth.

[0005] In the complexation reaction of GHK with copper ions, existing synthesis techniques still face numerous challenges, including unsatisfactory product yields, high emissions of waste, uneven product particle size distribution, and inconsistent appearance and color. These problems not only significantly increase the technical risks and production costs during process scale-up, resulting in high prices for end products, but also severely restrict the expansion of its application scope and market promotion. Therefore, this invention proposes a method for synthesizing copper peptides to at least partially solve the problems that may exist in the existing techniques. Summary of the Invention

[0006] In view of the above problems, embodiments of the present invention are proposed to provide a method for synthesizing copper peptides that overcomes or at least partially solves the above problems.

[0007] To address the above problems, embodiments of the present invention disclose a method for synthesizing copper peptides, comprising:

[0008] S1. Keep the temperature inside the reactor at 0-5℃, dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride in 0.1-2 L of water to obtain the reaction solution;

[0009] S2. Adjust the pH of the reaction solution to 6.0–10.0 using alkaline materials;

[0010] S3. Add (0.9-3 equivalents) of copper salt, adjust the speed to 40-120 rpm, stir until dissolved, maintain the temperature at 5-40℃ for crystallization for 12-36 hours to obtain synthetic blue copper peptide crystals.

[0011] Optionally, after step S3, adding 0.9–3 equivalents of copper salt, adjusting the rotation speed to 40–120 rpm, stirring until dissolved, and maintaining the temperature at 5–40°C for crystallization for 12–36 hours to obtain synthetic blue copper peptide crystals, the process further includes:

[0012] S4. Cool to 0 to -5℃ and let stand for 6 to 18 hours, then filter, and dry at 40 to 80℃ for 12 to 48 hours to obtain synthetic blue copper peptide crystals with a moisture content of 2 to 8% and a yield of 40 to 93%.

[0013] Optionally, in step S1, the temperature inside the reaction vessel is maintained at 0–5°C, and 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride is dissolved in 0.1–2 L of water to obtain a reaction solution comprising:

[0014] Maintain the temperature inside the reactor at 0–5°C, and dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride in 0.5 L, 1 L, or 1.2 L of water to obtain the reaction solution.

[0015] Optionally, step S2, adjusting the pH of the reaction solution to 6.0–10.0 using an alkaline material, includes:

[0016] The pH of the reaction solution is adjusted to 6.0–10.0 using one of the following: sodium carbonate, potassium carbonate, sodium hydroxide, potassium hydroxide, lithium hydroxide, triethylamine, or N,N-diisopropylethylamine.

[0017] Optionally, in step S3, 0.9–3 equivalents of copper salt are added, the stirring speed is adjusted to 40–120 rpm, and the mixture is stirred until dissolved. The temperature is maintained at 5–40°C for crystallization for 12–36 hours to obtain synthetic blue copper peptide crystals, comprising:

[0018] Add 0.9–3 equivalents of one or two of copper chloride, basic copper carbonate, copper acetate, and copper hydroxide, and stir until dissolved. Adjust the rotation speed to 40–120 rpm and maintain the temperature at 5–40°C for crystallization for 12–36 hours to obtain synthetic blue copper peptide crystals.

[0019] Optionally, step S2, adjusting the pH of the reaction solution to 6.0–10.0 using an alkaline material, includes:

[0020] Adjust the pH of the reaction solution to 6.0, 8.0, or 10.0 using an aqueous sodium hydroxide solution; or,

[0021] The pH of the reaction solution was adjusted to 8.0 using triethylamine; or,

[0022] Adjust the pH of the reaction solution to 8.0 using potassium hydroxide; or,

[0023] Adjust the pH of the reaction solution to 8.0 using lithium hydroxide; or,

[0024] The pH of the reaction solution was adjusted to 8.0 using sodium carbonate; or,

[0025] The pH of the reaction solution was adjusted to 8.0 using sodium carbonate; or,

[0026] The pH of the reaction solution was adjusted to 8.0 using N,N-diisopropylethylamine.

[0027] Optionally, in step S3, 0.9–3 equivalents of copper salt are added, the stirring speed is adjusted to 40–120 rpm, and the mixture is stirred until dissolved. The temperature is maintained at 5–40°C for crystallization for 12–36 hours to obtain synthetic blue copper peptide crystals, comprising:

[0028] Add 1.0 equivalent of basic copper carbonate, copper hydroxide, copper chloride, or copper acetate, adjust the stirring speed to 100 rpm, stir until dissolved, maintain the temperature at 35℃±1℃, and crystallize for 32 hours to obtain synthetic blue copper peptide crystals; or,

[0029] Add 1.0 equivalent of basic copper carbonate, adjust the stirring speed to 60 or 140 rpm, stir until dissolved, maintain the temperature at 35℃±1℃, and crystallize for 32 hours to obtain synthetic blue copper peptide crystals; or,

[0030] Add 1.0 equivalent of basic copper carbonate, adjust the stirring speed to 100 rpm, stir until dissolved, maintain the temperature at 40℃±1℃, and crystallize for 32 hours to obtain synthetic blue copper peptide crystals; or,

[0031] Add 1.0 equivalent of basic copper carbonate, adjust the stirring speed to 100 rpm, stir until dissolved, maintain the temperature at 5℃±1℃, and crystallize for 32 hours to obtain synthetic blue copper peptide crystals; or,

[0032] Add 1.0 equivalent of basic copper carbonate, adjust the speed to 100 rpm, stir until dissolved, maintain the temperature at 35℃±1℃, and crystallize for 16 or 24 hours to obtain synthetic blue copper peptide crystals.

[0033] Optionally, in step S4, after cooling to 0 to -5°C and standing for 6 to 18 hours, followed by filtration, the product is dried at 40 to 80°C for 12 to 48 hours to obtain synthetic blue copper peptide crystals with a moisture content of 2 to 8% and a yield of 40 to 93%, comprising:

[0034] After cooling to 0–-5℃ and standing for 6–18 hours, the mixture is filtered and then dried in a forced-air drying oven or vacuum oven at 40–80℃ for 12–48 hours to obtain synthetic blue copper peptide crystals with a moisture content of 2–8% and a yield of 40–93%. Specifically, this includes:

[0035] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.3%, a purity of 99.41%, and a yield of 87.0%; or,

[0036] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.4%, a purity of 99.52%, and a yield of 73.0%; or,

[0037] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.0%, a purity of 98.3%, and a yield of 92.8%; or,

[0038] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.8% and a yield of 78.6%; or,

[0039] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 4.9%, a purity of 87.9%, and a yield of 32.6%; or,

[0040] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.2%, a purity of 98.6%, and a yield of 81.7%; or,

[0041] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 97.7%, and a yield of 67.3%; or,

[0042] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 98.1%, and a yield of 80%; or,

[0043] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 97.7%, and a yield of 67.3%; or,

[0044] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.4%, a purity of 83.4%, and a yield of 71.0%; or,

[0045] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.8%, a purity of 99.3%, and a yield of 89.6%; or,

[0046] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.1%, a purity of 98.3%, and a yield of 59.5%; or,

[0047] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.1%, a purity of 95.6%, and a yield of 73.1%; or,

[0048] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 6.0%, a purity of 99.41%, and a yield of 88.0%; or,

[0049] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 6.0%, a purity of 99.41%, and a yield of 88.0%; or,

[0050] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 99.41%, and a yield of 87.0%; or,

[0051] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 96.7%, and a yield of 75.9%; or,

[0052] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 97.7%, and a yield of 40.7%; or,

[0053] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.8%, a purity of 98.37%, and a yield of 33.7%; or,

[0054] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.1%, a purity of 99.12%, and a yield of 81.9%; or,

[0055] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.5%, a purity of 99.21%, and a yield of 37.3%; or,

[0056] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.5%, a purity of 99.19%, and a yield of 84.8%; or,

[0057] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 2.8%, a purity of 95.36%, and a yield of 86.3%; or,

[0058] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 13.7%, a purity of 98.72%, and a yield of 85.6%; or,

[0059] After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 11.7%, a purity of 99.33%, and a yield of 86.2%.

[0060] This invention offers the following advantages: 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride is dissolved in 0.1-2 L of water while maintaining the reaction vessel temperature at 0-5°C to obtain a reaction solution. The pH of the reaction solution is adjusted to 6.0-10.0 using an alkaline material. 0.9-3 equivalents of copper salt are added, and the stirring speed is adjusted to 40-120 rpm until dissolved. The mixture is then crystallized at 5-40°C for 12-36 hours to obtain synthetic blue copper peptide crystals. The copper peptide synthesis method of this application is simple to operate, operates under mild conditions, and is suitable for industrial production. It boasts high yields, generally exceeding 80%. The product quality is stable, with controllable moisture content. The crystals exhibit good morphology and a certain luster. Furthermore, it has good solubility, facilitating subsequent applications. Attached Figure Description

[0061] Figure 1 This is an HPLC chromatogram of a method for synthesizing copper peptides according to an embodiment of the present invention;

[0062] Figure 2 This is a crystal diagram of the first copper peptide obtained by a method for synthesizing copper peptide according to an embodiment of the present invention.

[0063] Figure 3 This is a crystal diagram of a second copper peptide prepared by a method for synthesizing copper peptide according to an embodiment of the present invention.

[0064] Figure 4 This is a crystal diagram of the third copper peptide obtained by a method for synthesizing copper peptides according to an embodiment of the present invention. Detailed Implementation

[0065] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0066] An embodiment of a method for synthesizing copper peptides provided by the present invention may specifically include:

[0067] Maintain the temperature inside the reactor at 0–5°C, and dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 0.1–2 L of water to obtain the reaction solution;

[0068] The pH of the reaction solution was adjusted to 6.0–10.0 using alkaline materials.

[0069] Add (0.9–3 equivalents) of copper salt, adjust the rotation speed to 40–120 rpm, stir until dissolved, maintain the temperature at 5–40℃ for crystallization for 12–36 hours to obtain synthetic blue copper peptide crystals.

[0070] It also includes: S4, cooling to 0 to -5℃ and standing for 6 to 18 hours, then filtering, and drying at 40 to 80℃ for 12 to 48 hours to obtain synthetic blue copper peptide crystals with a moisture content of 2 to 8% and a yield of 40 to 93%.

[0071] By maintaining the temperature inside the reactor at 0–5°C, 100 g (0.265 mol) of glycyl-histyl-lysine hydrochloride (GHK.HCl) is dissolved in 0.1–2 L of water. The pH of the reaction solution is adjusted to 6.0–10.0 using one of the following bases: sodium carbonate, potassium carbonate, sodium hydroxide, potassium hydroxide, lithium hydroxide, triethylamine, or N,N-diisopropylethylamine. One or two of the following copper salts (copper chloride, basic copper carbonate, copper acetate, or copper hydroxide) are added and stirred until dissolved. The stirring speed is adjusted to 40–120 rpm, and the temperature is maintained at 5–40°C for crystallization for 12–36 hours. After crystallization, the temperature is lowered to 0–-5°C and allowed to stand for 6–18 hours. The mixture is then filtered and dried at 40–80°C (using a forced-air or vacuum oven) for 12–48 hours. The moisture content is measured to be 2–8%, and the yield is 40–93%.

[0072] The beneficial effects of this application include: simple operation, mild conditions, suitable for industrial production; high yield, generally reaching over 80%; stable product quality, controllable moisture content; good crystal morphology, with a certain luster; good solubility, facilitating subsequent applications.

[0073] Below, in conjunction with the appendix Figure 1 The method for synthesizing a copper peptide in this exemplary embodiment will be further described below.

[0074] Example 1:

[0075] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool to 0–-5°C and let stand for 12 hours. Filter, and dry in a 65°C forced-air oven for 36 hours. The moisture content is 5.3%. The product is dark blue, uniformly distributed, and has a certain luster, with small crystal particles and a purity of 99.41% (e.g., ...). Figure 2 As shown in the figure, the yield was 87.0%.

[0076] Example 2:

[0077] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.2 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content was 5.4%. The product was dark blue, uniformly distributed crystal particles with a certain luster, with a purity of 99.52% and a yield of 73.0%.

[0078] Example 3:

[0079] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 0.5 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.0%. The product is a dark blue, uniformly distributed crystalline powder with a certain luster, with a purity of 98.3% and a yield of 92.8%.

[0080] Example 4:

[0081] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 6.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content was 5.8%. The product was dark blue, uniformly distributed crystal particles with a certain luster, with a purity of 94.3% and a yield of 78.6%.

[0082] Example 5:

[0083] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 10.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content was found to be 4.9%. The product consists of uniformly distributed, grayish-blue crystal particles with a certain luster, with a purity of 87.9% and a yield of 32.6%.

[0084] Example 6:

[0085] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of copper hydroxide and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content was 5.2%. The product consists of uniformly distributed, grayish-blue crystal particles with a certain luster, with a purity of 98.6% and a yield of 81.7%.

[0086] Example 7:

[0087] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of copper chloride and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.7%. The product consists of uniformly distributed, grayish-blue crystal particles with a certain luster, with a purity of 97.7% and a yield of 67.3%.

[0088] Example 8:

[0089] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of copper acetate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.7%. The product is a uniformly distributed, grayish-blue crystal with a certain luster. The product has an acetic acid odor, a purity of 98.1%, and a yield of 80.0%.

[0090] Example 9:

[0091] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with triethylamine. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.7%. The product consists of uniformly distributed, grayish-blue crystal particles with a certain luster, with a purity of 97.7% and a yield of 67.3%.

[0092] Example 10:

[0093] Maintaining the temperature inside the reactor at 0–5°C, 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) was dissolved in 1.0 L of water. The pH of the reaction solution was adjusted to 8.0 with triethylamine. 1.0 equivalent of basic copper carbonate was added and stirred until dissolved. The stirring speed was adjusted to 100 rpm, and the temperature was maintained at 35°C ± 1°C for crystallization for 32 hours. After crystallization, the temperature was lowered to 0–-5°C and allowed to stand for 12 hours. The mixture was then filtered and dried in a 65°C forced-air oven for 36 hours. The moisture content was 5.4%. The product consisted of uniformly distributed, grayish-blue crystal particles with a certain luster, with a purity of 83.4% and a yield of 71.0%.

[0094] Example 11:

[0095] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with potassium hydroxide. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content was 5.4%. The product consists of uniformly distributed, grayish-blue crystal particles with a certain luster, with a purity of 98.9% and a yield of 83.2%.

[0096] Example 11:

[0097] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with lithium hydroxide. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.8%. The product consists of uniformly distributed, grayish-blue crystal particles with a certain luster, with a purity of 99.3% and a yield of 89.6%.

[0098] Example 12:

[0099] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium carbonate. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content was 5.1%. The product consisted of uniformly distributed blue crystal particles with a certain luster, with a purity of 98.3% and a yield of 59.5%.

[0100] Example 13:

[0101] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with potassium carbonate. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.1%. The product consists of uniformly distributed blue crystal particles with a certain luster, with a purity of 95.6% and a yield of 73.2%.

[0102] Example 14:

[0103] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with N,N-diisopropylethylamine. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content was 5.1%. The product consisted of uniformly distributed, grayish-blue crystal particles with a certain luster, with a purity of 91.8% and a yield of 56.6%.

[0104] Example 15:

[0105] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 140 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool to 0–-5°C and let stand for 12 hours. Filter, and dry in a 65°C forced-air oven for 36 hours. The moisture content is measured to be 6.0%. The product is a dark blue powder with a purity of 99.41% (e.g., ...). Figure 4 As shown in the figure, the yield was 88.0%.

[0106] Example 16:

[0107] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 60 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.7%. The product is dark blue, lustrous crystals with a 40-mesh particle size, a purity of 99.41%, and a yield of 87.0%.

[0108] Example 17:

[0109] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 40°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.7%. The product is dark blue, lustrous crystal particles with a purity of 96.7% and a yield of 75.9%.

[0110] Example 18:

[0111] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 5°C ± 1°C for crystallization for 32 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.7%. The product is dark blue, lustrous crystal particles with a purity of 97.7% and a yield of 40.7%.

[0112] Example 19:

[0113] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 16 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.8%. The product is a dark blue, uniformly distributed crystal with glossy small particles, with a purity of 98.37% and a yield of 33.7%.

[0114] Example 20:

[0115] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 24 hours. Then, cool the solution to 0–-5°C and let it stand for 12 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.1%. The product is a dark blue, uniformly distributed, lustrous small crystal particle with a purity of 99.12% and a yield of 81.9%.

[0116] Example 21:

[0117] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 24 hours. Then, cool the solution to 0–-5°C and let it stand for 6 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.5%. The product is a dark blue, uniformly distributed, shiny small crystal particle with a purity of 99.21% and a yield of 37.3%.

[0118] Example 22:

[0119] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 24 hours. Then, cool the solution to 0–-5°C and let it stand for 10 hours. Filter the solution and dry it in a 65°C forced-air oven for 36 hours. The moisture content is 5.5%. The product is a dark blue, uniformly distributed, shiny small crystal particle with a purity of 99.19% and a yield of 84.8%.

[0120] Example 23:

[0121] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 24 hours. Then, cool to 0–-5°C and let stand for 10 hours. Filter, and dry in an 80°C forced-air oven for 36 hours. The moisture content is measured to be 2.8%. The product is light blue crystalline granules (e.g., ...). Figure 3 As shown in the figure, the purity is 95.36% and the yield is 86.3%.

[0122] Example 24:

[0123] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 24 hours. Then, cool the solution to 0–-5°C and let it stand for 10 hours. Filter the solution and dry it in a 40°C forced-air oven for 36 hours. The moisture content was 13.7%. The product was light blue granules with a purity of 98.72% and a yield of 85.6%.

[0124] Example 25:

[0125] Maintain the temperature inside the reactor at 0–5°C. Dissolve 100 g (0.265 mol) glycyl-histyl-lysine hydrochloride (GHK.HCl) in 1.0 L of water. Adjust the pH of the reaction solution to 8.0 with sodium hydroxide aqueous solution. Add 1.0 equivalent of basic copper carbonate and stir until dissolved. Adjust the stirring speed to 100 rpm and maintain the temperature at 35°C ± 1°C for crystallization for 24 hours. Then, cool the solution to 0–-5°C and let it stand for 10 hours. Filter the solution and dry it in a 65°C forced-air oven for 24 hours. The moisture content is 11.7%. The product is dark blue crystalline granules with a certain luster, with a purity of 99.33% and a yield of 86.2%.

[0126] It should be noted that the aforementioned n-fold equivalent refers to the amount of alkaline substance added, which, in terms of chemical reactivity, is equal to the amount required for complete reaction of 100 grams (0.265 mol) of the reagent. In this application, it refers to the amount of copper salt used relative to GHK.HCl, where the alkali is only used to adjust the pH value. For example, the amount of alkaline material used for 1.0 equivalent is 0.265 mol, while that for 0.3 equivalent is 0.3 × 0.265 mol. Furthermore, although the color change of copper peptides is directly and significantly related to their water content, it is not the only factor. For example, physical state and impurities can also affect the color. Therefore, the small copper peptide crystal particles of this application will exhibit different depths of color.

[0127] Although preferred embodiments of the present invention have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of the embodiments of the present invention.

[0128] Finally, it should be noted that in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or terminal device that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or terminal device. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or terminal device that includes said element.

[0129] The above provides a detailed description of a method for synthesizing copper peptides provided by the present invention. Specific examples have been used to illustrate the principles and implementation methods of the present invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of the present invention. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of the present invention. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. A method for synthesizing copper peptides, characterized in that, include: Maintain the temperature inside the reactor at 0–5°C, and dissolve 100 g of glycyl-histyl-lysine hydrochloride in 0.1–2 L of water to obtain the reaction solution; The pH of the reaction solution was adjusted to 6.0–10.0 using alkaline materials. Add 0.9–3 equivalents of copper salt, adjust the speed to 40–120 rpm, stir until dissolved, maintain the temperature at 5–40℃ for crystallization for 12–36 hours to obtain synthetic blue copper peptide crystals.

2. The apparatus according to claim 1, characterized in that, After adding 0.9–3 equivalents of copper salt, adjusting the stirring speed to 40–120 rpm, stirring until dissolved, and maintaining the temperature at 5–40°C for crystallization for 12–36 hours to obtain synthetic blue copper peptide crystals, the process further includes: After cooling to 0 to -5℃ and standing for 6 to 18 hours, the mixture is filtered and then dried at 40 to 80℃ for 12 to 48 hours to obtain synthetic blue copper peptide crystals with a moisture content of 2 to 8% and a yield of 40 to 93%.

3. The apparatus according to claim 1, characterized in that, The temperature inside the reaction vessel is maintained at 0–5°C. 100 g of glycyl-histyl-lysine hydrochloride is dissolved in… The reaction solution is obtained in 0.1–2 L of water, comprising: Maintain the temperature inside the reactor at 0–5°C, and dissolve 100 g of glycyl-histyl-lysine hydrochloride in… The reaction solution is obtained by adding 0.5L, 1L, or 1.2L of water.

4. The apparatus according to claim 1, characterized in that, The step of adjusting the pH of the reaction solution to 6.0–10.0 using an alkaline material includes: The pH of the reaction solution is adjusted to 6.0–10.0 using one of the following: sodium carbonate, potassium carbonate, sodium hydroxide, potassium hydroxide, lithium hydroxide, triethylamine, or N,N-diisopropylethylamine.

5. The apparatus according to claim 1, characterized in that, The process involves adding 0.9–3 equivalents of copper salt, adjusting the stirring speed to 40–120 rpm, stirring until dissolved, and maintaining the temperature at 5–40°C for crystallization for 12–36 hours to obtain synthetic blue copper peptide crystals, comprising: Add 0.9–3 equivalents of one or two of copper chloride, basic copper carbonate, copper acetate, and copper hydroxide, and stir until dissolved. Adjust the rotation speed to 40–120 rpm and maintain the temperature at 5–40°C for crystallization for 12–36 hours to obtain synthetic blue copper peptide crystals.

6. The apparatus according to claim 1 or 4, characterized in that, The step of adjusting the pH of the reaction solution to 6.0–10.0 using an alkaline material includes: Adjust the pH of the reaction solution to 6.0, 8.0, or 10.0 using an aqueous sodium hydroxide solution; or, The pH of the reaction solution was adjusted to 8.0 using triethylamine; or, Adjust the pH of the reaction solution to 8.0 using potassium hydroxide; or, Adjust the pH of the reaction solution to 8.0 using lithium hydroxide; or, The pH of the reaction solution was adjusted to 8.0 using sodium carbonate; or, The pH of the reaction solution was adjusted to 8.0 using sodium carbonate; or, The pH of the reaction solution was adjusted to 8.0 using N,N-diisopropylethylamine.

7. The apparatus according to claim 1 or 5, characterized in that, The process involves adding 0.9–3 equivalents of copper salt, adjusting the stirring speed to 40–120 rpm, stirring until dissolved, and maintaining the temperature at 5–40°C for crystallization for 12–36 hours to obtain synthetic blue copper peptide crystals, comprising: Add 1.0 equivalent of basic copper carbonate, copper hydroxide, copper chloride, or copper acetate, adjust the stirring speed to 100 rpm, stir until dissolved, maintain the temperature at 35℃±1℃, and crystallize for 32 hours to obtain synthetic blue copper peptide crystals; or, Add 1.0 equivalent of basic copper carbonate, adjust the stirring speed to 60 or 140 rpm, stir until dissolved, maintain the temperature at 35℃±1℃, and crystallize for 32 hours to obtain synthetic blue copper peptide crystals; or, Add 1.0 equivalent of basic copper carbonate, adjust the stirring speed to 100 rpm, stir until dissolved, maintain the temperature at 40℃±1℃, and crystallize for 32 hours to obtain synthetic blue copper peptide crystals; or, Add 1.0 equivalent of basic copper carbonate, adjust the stirring speed to 100 rpm, stir until dissolved, maintain the temperature at 5℃±1℃, and crystallize for 32 hours to obtain synthetic blue copper peptide crystals; or, Add 1.0 equivalent of basic copper carbonate, adjust the speed to 100 rpm, stir until dissolved, maintain the temperature at 35℃±1℃, and crystallize for 16 or 24 hours to obtain synthetic blue copper peptide crystals.

8. The apparatus according to claim 2, characterized in that, The process involves cooling to 0–-5°C, allowing to stand for 6–18 hours, filtering, and then drying at 40–80°C for 12–48 hours to obtain synthetic blue copper peptide crystals with a moisture content of 2–8% and a yield of 40–93%, comprising: After cooling to 0–-5℃ and standing for 6–18 hours, the mixture is filtered and then dried in a forced-air drying oven or vacuum oven at 40–80℃ for 12–48 hours to obtain synthetic blue copper peptide crystals with a moisture content of 2–8% and a yield of 40–93%. Specifically, this includes: After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.3%, a purity of 99.41%, and a yield of 87.0%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.4%, a purity of 99.52%, and a yield of 73.0%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.0%, a purity of 98.3%, and a yield of 92.8%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.8% and a yield of 78.6%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 4.9%, a purity of 87.9%, and a yield of 32.6%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.2%, a purity of 98.6%, and a yield of 81.7%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 97.7%, and a yield of 67.3%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 98.1%, and a yield of 80%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 97.7%, and a yield of 67.3%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.4%, a purity of 83.4%, and a yield of 71.0%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.8%, a purity of 99.3%, and a yield of 89.6%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.1%, a purity of 98.3%, and a yield of 59.5%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.1%, a purity of 95.6%, and a yield of 73.1%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 6.0%, a purity of 99.41%, and a yield of 88.0%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 6.0%, a purity of 99.41%, and a yield of 88.0%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 99.41%, and a yield of 87.0%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 96.7%, and a yield of 75.9%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.7%, a purity of 97.7%, and a yield of 40.7%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.8%, a purity of 98.37%, and a yield of 33.7%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.1%, a purity of 99.12%, and a yield of 81.9%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.5%, a purity of 99.21%, and a yield of 37.3%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 5.5%, a purity of 99.19%, and a yield of 84.8%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 2.8%, a purity of 95.36%, and a yield of 86.3%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 13.7%, a purity of 98.72%, and a yield of 85.6%; or, After cooling to 0 to -5℃ and standing for 12 hours, the mixture was filtered and then dried in a forced-air drying oven at 65℃ for 36 hours to obtain synthetic blue copper peptide crystals with a moisture content of 11.7%, a purity of 99.33%, and a yield of 86.2%.

Images