Single-hole access enzyme-linked immunosorbent assay device

Abstract

The invention aims to provide single-hole access enzyme-linked immunosorbent assay equipment, and relates to the technical field of experimental detection equipment, the single-hole access enzyme-linked immunosorbent assay equipment comprises a reaction device, the reaction device comprises a reaction plate and a hole plate, and the reaction device can provide a reaction field during enzyme-linked immunosorbent assay; the fixing device comprises a control button and a mounting plate and can fix and limit the reaction device, prevent the reaction device from moving and ensure stable reaction; the constant temperature device comprises a cover body, an electric push rod and a sealing strip, so that the reaction device is stabilized in a required temperature interval, and the problems that a traditional enzyme-linked immunosorbent assay device is relatively low in efficiency when being used for treating a small amount of samples or a plurality of detection items and is difficult to meet high-throughput or diversified detection requirements are solved.

Description

Technical Field

[0001] This invention relates to the field of experimental detection equipment technology, and in particular to a single-well access enzyme-linked immunosorbent assay (ELISA) device. Background Technology

[0002] Traditional ELISA instruments typically process samples on a per-plate basis, meaning a single operation involves reacting and detecting on a complete 96-well or 384-well plate. This processing method is essentially batch processing, implying that all samples must undergo steps such as loading, incubation, washing, and reading together. Due to the per-plate design, the system cannot flexibly perform parallel detection of multiple items on a single sample tube, resulting in low efficiency when processing small numbers of samples or multiple detection items, making it difficult to meet high-throughput or diversified detection needs.

[0003] For example, CN202422730479.6 proposes a magnetic porous reaction plate, which includes a porous plate, a base, a cover plate, and a magnet. The magnet can be fitted between the porous plate and the base and fits the shape of the bottom of the porous plate. This utility model can perform operations such as moving, centrifuging, and shaking of the porous reaction plate while maintaining magnetic force, thereby realizing more flexible and diverse experimental designs and experimental schemes. However, it is less efficient when processing a small number of samples or multiple detection items, and it is difficult to meet the needs of high-throughput or diversified detection, thus failing to solve the above problems.

[0004] For example, CN202421660514.5 proposes a temperature-adjustable reaction plate, comprising: a cover plate, a reaction plate body, and a base plate; the reaction plate body is provided with a temperature control structure and placement holes, the temperature control structure includes a fluid channel disposed on the reaction plate body and connectors respectively disposed at the inlet and outlet of the fluid channel, the connectors being used for the inflow and / or outflow of external fluid; a plurality of placement holes are arranged sequentially on the reaction plate body and penetrate the reaction plate body, the placement holes being provided with reaction flasks; the cover plate and the base plate are matched in shape to the reaction plate body and are both disposed on the reaction plate body, the cover plate and the base plate being located at both ends of the placement holes respectively. When using the reaction plate provided by this utility model, the temperature of the environment in which the chemical reaction is taking place in the reaction flask carried by the reaction plate body can be controlled by controlling the temperature of the external fluid, thus meeting the temperature conditions required for heat-driven chemical reactions. However, the efficiency is low when processing a small number of samples or multiple detection items, making it difficult to meet the needs of high-throughput or diversified detection, and the above problems are not solved.

[0005] The present invention can effectively solve the drawbacks of the traditional ELISA instrument's whole-plate processing mode through the provided reaction device, realize single-well storage and analysis function, improve efficiency, and meet high-throughput or diversified detection needs. Summary of the Invention

[0006] Technical problems to be solved To address the shortcomings of existing technologies, this invention provides a single-well access enzyme-linked immunosorbent assay (ELISA) device, which solves the problem that traditional ELISA devices are inefficient when processing a small number of samples or multiple detection items, making it difficult to meet high-throughput or diversified detection needs.

[0007] Technical solution To achieve the above objectives, the present invention provides the following technical solution: a single-well retrieval enzyme-linked immunosorbent assay (ELISA) device, comprising a loading tray fixed on the work site, a robotic arm installed in the center of the loading tray, and further comprising: a reaction device located at the edge of the loading tray to provide a reaction site for ELISA analysis; a fixing device located below the reaction device to fix and limit the reaction device, prevent the reaction device from moving, and ensure reaction stability; and a temperature control device located above the reaction device to ensure that the reaction device is stable within the required temperature range.

[0008] Furthermore, the reaction apparatus includes a reaction plate and an orifice plate, with the reaction plate placed on a loading tray and the orifice plate placed on the reaction plate.

[0009] Furthermore, the reaction plate has a central recess with multiple evenly distributed through holes that fit into the perforated plate. Support strips are provided on both sides of the through holes. Different chamfers or rounded corners are provided at both ends of the reaction plate. A mark is provided above one end of the reaction plate. Detection holes are provided at the edge of the reaction plate. A support part is provided below the reaction plate.

[0010] Furthermore, the orifice plate is composed of multiple reaction holes connected by a connecting part. The orifice plate is made of transparent material. The two ends of the orifice plate are respectively provided with a first hand-held part and a second hand-held part with different structures. A mark is provided below the second hand-held part. The upper surface of the orifice plate and the upper surface of the reaction plate are on the same plane.

[0011] Furthermore, the bottom of the reaction hole can be a "V" angle of 110° and 90°, or a "U" shape.

[0012] Furthermore, the connecting part is provided with a groove and a protrusion in the middle, and the perforated plate can be broken off and separated into individual reaction holes through the groove and protrusion of the connecting part.

[0013] Furthermore, the fixing device includes a control button and a mounting plate. The control button is fixed on the loading plate, and the mounting plate is fixed on the loading plate. The mounting plate has a protruding positioning strip that corresponds to the reaction plate. A sensor is provided in the middle of the mounting plate to detect whether a reaction plate is installed on the mounting plate. A suction tube is provided on the mounting plate, and the position of the suction tube corresponds to the position of the support part on the reaction plate. A top block is provided at the bottom of the mounting plate to hold the middle of the suction tube. A vacuum generator, a five-way connector, and an electromagnetic push-pull valve are fixed at the bottom of the mounting plate. A push plate is also slidably installed. The vacuum generator has an air inlet, a negative pressure port, and a silencer. The air inlet of the vacuum generator is connected to an external air pump. The negative pressure port of the vacuum generator is connected to the five-way connector through a conduit. The five-way connector is connected to the suction tube through a conduit.

[0014] Furthermore, the push plate is fixed to the telescopic rod of the electromagnetic push-pull valve, and the push plate has protrusions on both sides corresponding to the position of the top block for squeezing the middle of the straw. The straw is made of soft and elastic materials including silicone, and the upper end of the straw has a thin edge structure.

[0015] Furthermore, the constant temperature device includes a cover, an electric actuator, and a sealing strip. The cover is rotatably mounted on the loading tray, and the electric actuator is located between the cover and the loading tray. The sealing strip is fixed to the mounting plate. The cover is made of transparent material and is located above the reaction device. One end of the cover has an air inlet and the other end has an air outlet. The air inlet is connected to an external air pump and a heating device through a conduit, and the air outlet is connected to an exhaust device through a conduit. A temperature sensor is also provided on the cover.

[0016] Furthermore, the outer side of the sealing strip is provided with a raised structure with one end higher than the other, and the inner side of the sealing strip is provided with an annular raised structure. The two raised structures form a groove structure to fit the size of the cover.

[0017] Beneficial effects The present invention has the following beneficial effects: (1) The single-well access enzyme-linked immunosorbent assay device of the present invention realizes the single-well access analysis function, breaks the limitation of the traditional ELISA instrument whole plate processing, and the design of the reaction device makes it possible to separate a single reaction well by breaking through the connection part, which facilitates the operation of a single sample and greatly improves the flexibility when processing a small number of samples or multiple detection items.

[0018] (2) The fixing device can accurately fix the limiting reaction device through the coordinated action of components such as positioning strip, sensor, suction tube and vacuum generator, preventing it from moving during the reaction process, ensuring the stability and accuracy of the reaction, and thus improving the reliability of the detection results.

[0019] (3) The constant temperature device uses a cover, electric push rod, sealing strip, air inlet, air outlet, heating device, exhaust device and temperature sensor to stabilize the reaction device in the required temperature range, ensure the smooth progress of the reaction and ensure the validity of the test results.

[0020] Of course, any product implementing this invention does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is the invention Figure 1 A magnified view of point A; Figure 3 The explosion of the reaction device and the temperature control device of the present invention. Figure 1 ; Figure 4 The explosion of the reaction device and the temperature control device of the present invention. Figure 2 ; Figure 5 This is an exploded view of the reaction plate and orifice plate of the reaction apparatus of the present invention; Figure 6 This is a schematic diagram of the structure of the reaction plate of the reaction device of the present invention; Figure 7 This is a schematic diagram of the orifice plate structure of the reaction device of the present invention. Figure 1 ; Figure 8 This is a schematic diagram of the orifice plate structure of the reaction device of the present invention. Figure 2 ; Figure 9 This is a cross-sectional view of an embodiment of the reaction orifice of the reaction apparatus of the present invention; Figure 10 This is a schematic diagram of the fixing device of the present invention. Figure 1 ; Figure 11 This is a cross-sectional view of the sealing strip of the constant temperature device of the present invention; Figure 12 This is a schematic diagram of the fixing device of the present invention. Figure 2 ; Figure 13 This is a cross-sectional view of the suction tube of the fixing device of the present invention; Figure 14 This is a schematic diagram of the push plate of the fixing device of the present invention.

[0022] Reference numerals: 1. Loading tray; 2. Robotic arm; 3. Control button; 4. Mounting plate; 5. Cover; 6. Electric push rod; 7. Sealing strip; 8. Reaction plate; 9. Orifice plate; 401. Positioning strip; 402. Sensor; 403. Vacuum generator; 404. Five-way connector; 405. Electromagnetic push-pull valve; 406. Push plate; 407. Suction tube; 408. Top block; 501. Air inlet; 502. Air outlet; 503. Temperature sensor; 801. Support part; 802. Detection hole; 803. Support strip; 901. Reaction hole; 902. Connecting part; 903. First hand-held part; 904. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] Please see Figure 1 - Figure 14 This invention provides a technical solution: a single-well retrieval enzyme-linked immunosorbent assay (ELISA) device includes a loading tray 1, which is fixed on the work site. A robotic arm 2 is installed in the middle of the loading tray 1. The device also includes: a reaction device located at the edge of the loading tray 1, which provides a reaction site for ELISA analysis; a fixing device located below the reaction device, which can fix and limit the reaction device to prevent it from moving and ensure reaction stability; and a temperature control device located above the reaction device, which ensures that the reaction device is stable within the required temperature range.

[0025] Preferably, the loading tray 1 is mounted on a rotating device, allowing the loading tray 1 to rotate, facilitating the operator to operate all reaction devices from a fixed position. The robotic arm 2 is fixed above the loading tray 1 by an external support device and is not connected to the loading tray 1, thus being unaffected by the rotation of the loading tray 1. Specifically, a working position is provided next to the loading tray 1, where the operator is located. The rotating device causes the loading tray 1 to rotate, switching between different reaction devices to perform multiple sets of different enzyme-linked immunosorbent assay (ELISA) experiments. The robotic arm 2 is controlled to perform the changing and transporting work, assisting in the ELISA experiments.

[0026] like Figure 3 - Figure 9As shown, the single-well ELISA analyzer includes a reaction device comprising a reaction plate 8 and well plates 9. The reaction plate 8 is placed on a loading tray 1, and the well plates 9 are placed on the reaction plate 8. The reaction plate 8 has a central recess with multiple evenly distributed through holes that fit the well plates 9. Support strips 803 are provided on both sides of the through holes to support the well plates 9, creating a gap between the well plates 9 and the reaction plate 8 and reducing contact between them to prevent adhesion. The reaction plate 8 has different chamfers or rounded corners at both ends for orientation. A mark is provided on the top of one end of the reaction plate 8 to distinguish the number of rows of through holes. Detection holes 802 are provided on the edge of the reaction plate 8 for ventilation to prevent the well plates 9 and the reaction plate 8 from sticking together due to air pressure. It can also be used in electronic vision devices. The identification and detection facilitates the gripping of the robotic arm 2. A support part 801 is provided below the reaction plate 8 to improve the structural strength and support force of the reaction plate 8. The perforated plate 9 is composed of multiple reaction holes 901 connected by a connecting part 902. The perforated plate 9 is made of transparent material. The two ends of the perforated plate 9 are respectively provided with a first hand-held part 903 and a second hand-held part 904 with different structures. The connecting part 902 has a groove and a protrusion in the middle to prevent liquid from flowing from the edge of one reaction hole 901 to another reaction hole 901. The lower part of the second hand-held part 904 is marked to facilitate the differentiation of the number of rows of reaction holes 901 without damaging the flatness of the upper surface of the second hand-held part 904, ensuring the effect of applying the coating during subsequent reaction and allowing for better contact with the coating. The upper surface of the perforated plate 9 and the upper surface of the reaction plate 8 are on the same plane.

[0027] Preferably, the bottom of reaction well 901 can be a 110° or 90° “V” angle, or a “U” shape, to meet different enzyme-linked immunosorbent assay (ELISA) experimental requirements.

[0028] In a specific embodiment: According to the usage requirements, the reaction plate 8 is placed on the loading tray 1, and the well plate 9 is placed in the through hole of the reaction plate 8 (this step can also be completed by controlling the robot arm 2, the robot arm 2 clamps the reaction plate 8 and places it on the loading tray 1, and then the robot arm 2 clamps the first hand part 903 and the second hand part 904 of the well plate 9, or clamps the side of the reaction hole 901, so that the well plate 9 is placed in the through hole of the reaction plate 8), and performs enzyme-linked immunosorbent assay (ELISA) experiment, and then the upper surface of the well plate 9 and the upper surface of the reaction plate 8 are covered by a membrane to avoid cross-contamination of the liquid in the reaction plate 8.

[0029] In another embodiment different from the above embodiment: before the experiment, a single reaction hole 901 is separated by breaking off the groove and protrusion of the connecting part 902, and then the single reaction hole 901 is placed on the reaction plate 8 for analysis experiment, further reducing the interference of adjacent samples, and theoretically realizing "single sample detection", which is suitable for precious samples.

[0030] like Figure 10 , Figure 12 , Figure 13 and Figure 14 As shown, the single-well ELISA analyzer includes a fixing device, which includes a control button 3 and a mounting plate 4. The control button 3 is fixed to the loading tray 1, and the mounting plate 4 is fixed to the loading tray 1. The mounting plate 4 has a protruding positioning strip 401, which corresponds to the reaction plate 8 and can position and limit the reaction plate 8 to prevent it from moving. A sensor 402 is provided in the middle of the mounting plate 4 to detect whether the reaction plate 8 is installed on the mounting plate 4. A pipette 407 is provided on the mounting plate 4, and the position of the pipette 407 corresponds to the position of the support part 801 on the reaction plate 8. A top block 408 is provided at the bottom of the mounting plate 4 to hold the middle of the pipette 407. The bottom of the mounting plate 4 is fixed. The device includes a vacuum generator 403, a five-way connector 404, and an electromagnetic push-pull valve 405. A push plate 406 is also slidably installed. The vacuum generator 403 has an air inlet, a negative pressure port, and a silencer. The air inlet of the vacuum generator 403 is connected to an external air pump. The negative pressure port of the vacuum generator 403 is connected to the five-way connector 404 through a conduit. The five-way connector 404 is connected to a suction tube 407 through a conduit. The push plate 406 is fixed to the telescopic rod of the electromagnetic push-pull valve 405. The push plate 406 has protrusions on both sides corresponding to the position of the top block 408, which are used to squeeze the middle of the suction tube 407. The suction tube 407 is made of a soft and elastic material, including silicone. The upper end of the suction tube 407 has a thin edge structure.

[0031] In a specific embodiment: During use, the external air pump is manually controlled by the control button 3 or automatically controlled by the external control center to supply air to the vacuum generator 403. The negative pressure port of the vacuum generator 403 generates negative pressure, which in turn generates negative pressure at the upper end of the suction tube 407 through the five-way connector 404 and the conduit, thereby adsorbing the support part 801 of the reaction plate 8, thus fixing the reaction plate 8 and preventing it from moving. During the process, the thin edge structure at the upper end of the suction tube 407 can better fit the support part 801 of the reaction plate 8, resulting in a better adsorption effect.

[0032] like Figure 2 , Figure 3 and Figure 4As shown, the single-well ELISA analyzer includes a temperature control device, which comprises a cover 5, an electric actuator 6, and a sealing strip 7. The cover 5 is rotatably mounted on the loading tray 1. The cover 5 and the loading tray 1 are connected by the electric actuator 6. The sealing strip 7 is fixed to the mounting plate 4. The cover 5 is made of transparent material and is located above the reaction device. One end of the cover 5 has an air inlet 501, and the other end has an air outlet 502. The air inlet 501 is connected to an external air pump and heating device through a conduit, and the air outlet 502 is connected to an exhaust device through a conduit. The cover 5 is also equipped with a temperature sensor 503 for detecting the temperature inside the cover 5. The outer side of the sealing strip 7 has a raised structure with one end higher than the other end, which facilitates the rotation of the cover 5 to open and close. The inner side of the sealing strip 7 has an annular raised structure. The two raised structures form a groove structure to fit the size of the cover 5.

[0033] In a specific embodiment: during use, after the reaction plate 8 is covered with a film, the electric push rod 6 is manually controlled by the control button 3 or automatically controlled by the external control center to push the cover 5 to rotate, so that the cover 5 covers the sealing strip 7, thereby sealing the inside of the cover 5. Then, through the external air pump and heating device, hot air enters the cover 5 through the air inlet 501 on the cover 5. The reaction device in the cover 5 is in a constant temperature environment at a certain temperature, which is convenient for the enzyme-linked immunosorbent assay (ELISA) experiment.

[0034] Working principle: During use, place the reaction plate 8 on the loading tray 1 according to the usage requirements, and place the perforated plate 9 in the through hole of the reaction plate 8 (this step can also be completed by controlling the robot arm 2; the robot arm 2 clamps the reaction plate 8 and places it on the loading tray 1, and then the robot arm 2 clamps the first hand-held part 903 and the second hand-held part 904 of the perforated plate 9, or clamps the side of the reaction hole 901, so that the perforated plate 9 is placed in the through hole of the reaction plate 8). Then, manually control the external air pump to supply air to the vacuum generator 403 through the control button 3 or automatically control it through the external control center. The negative pressure port of the vacuum generator 403 generates negative pressure, thereby allowing the suction tube 407 to pass through the five-way connector 404 and the conduit. A negative pressure is generated at the top, which adsorbs the support part 801 of the reaction plate 8, thereby fixing the reaction plate 8 and preventing it from moving. Then, the enzyme-linked immunosorbent assay (ELISA) experiment is performed. The upper surface of the well plate 9 and the upper surface of the reaction plate 8 are covered by a membrane. Then, the electric push rod 6 is manually controlled by the control button 3 or automatically controlled by the external control center to push the cover 5 to rotate, so that the cover 5 covers the sealing strip 7, thereby sealing the inside of the cover 5. Then, hot air is introduced into the cover 5 through the air inlet 501 on the cover 5 by an external air pump and heating device. The reaction device in the cover 5 is in a constant temperature environment, which is conducive to the enzyme-linked immunosorbent assay (ELISA) experiment.

[0035] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0036] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A single-well retrieval enzyme-linked immunosorbent assay (ELISA) device, comprising a loading tray (1), the loading tray (1) being fixed on a work surface, and a robotic arm (2) being mounted in the middle of the loading tray (1), characterized in that, Also includes: The reaction device located at the edge of the loading tray (1) can provide a reaction site for enzyme-linked immunosorbent assay (ELISA). The fixing device located below the reaction apparatus can fix and limit the reaction apparatus, prevent the reaction apparatus from moving, and ensure the stability of the reaction. A temperature control device located above the reaction apparatus ensures that the reaction apparatus remains stable within the required temperature range.

2. The single-well access enzyme-linked immunosorbent assay (ELISA) device as described in claim 1, characterized in that, The reaction apparatus includes a reaction plate (8) and an orifice plate (9), with the reaction plate (8) placed on a loading tray (1) and the orifice plate (9) placed on the reaction plate (8).

3. The single-well access enzyme-linked immunosorbent assay (ELISA) device as described in claim 2, characterized in that, The reaction plate (8) is recessed in the middle, and multiple evenly distributed through holes are opened in the recess of the reaction plate (8). The through holes fit the hole plate (9). Support strips (803) are provided on both sides of the through holes. Different chamfers or rounded corners are provided at both ends of the reaction plate (8). A mark is provided above one end of the reaction plate (8). A detection hole (802) is provided on the edge of the reaction plate (8). A support part (801) is provided below the reaction plate (8).

4. The single-well access enzyme-linked immunosorbent assay (ELISA) device as described in claim 2, characterized in that, The orifice plate (9) is composed of multiple reaction holes (901) connected by a connecting part (902). The orifice plate (9) is made of transparent material. The two ends of the orifice plate (9) are respectively provided with a first hand-held part (903) and a second hand-held part (904) with different structures. The second hand-held part (904) is marked below. The upper surface of the orifice plate (9) and the upper surface of the reaction plate (8) are on the same plane.

5. The single-well access enzyme-linked immunosorbent assay (ELISA) device as described in claim 4, characterized in that, The bottom of the reaction hole (901) can be a "V" angle of 110° and 90°, or a "U" shape.

6. The single-well access enzyme-linked immunosorbent assay (ELISA) device as described in claim 4, characterized in that, The connecting part (902) has a groove and a protrusion in the middle, and the perforated plate (9) can be broken off and separated into a single reaction hole (901) through the groove and protrusion of the connecting part (902).

7. The single-well access enzyme-linked immunosorbent assay (ELISA) device as described in claim 1, characterized in that, The fixing device includes a control button (3) and a mounting plate (4). The control button (3) is fixed on the loading plate (1), and the mounting plate (4) is fixed on the loading plate (1). The mounting plate (4) has a protruding positioning strip (401) that corresponds to the reaction plate (8). A sensor (402) is provided in the middle of the mounting plate (4) to detect whether the reaction plate (8) is installed on the mounting plate (4). A suction tube (407) is provided on the mounting plate (4), and the position of the suction tube (407) corresponds to the position of the support part (801) on the reaction plate (8). The mounting plate (4) has a top block (408) at the bottom to hold the middle of the suction tube (407). The bottom of the mounting plate (4) is fixed with a vacuum generator (403), a five-way connector (404) and an electromagnetic push-pull valve (405). A push plate (406) is also slidably installed. The vacuum generator (403) has an air inlet, a negative pressure port and a silencer. The air inlet of the vacuum generator (403) is connected to an external air pump. The negative pressure port of the vacuum generator (403) is connected to the five-way connector (404) through a conduit. The five-way connector (404) is connected to the suction tube (407) through a conduit.

8. The single-well access enzyme-linked immunosorbent assay (ELISA) device as described in claim 7, characterized in that, The push plate (406) is fixed on the telescopic rod of the electromagnetic push-pull valve (405). The push plate (406) has protrusions on both sides corresponding to the position of the top block (408) for squeezing the middle of the straw (407). The straw (407) is made of soft and elastic materials including silicone. The upper end of the straw (407) has a thin edge structure.

9. The single-well access enzyme-linked immunosorbent assay (ELISA) device as described in claim 1, characterized in that, The constant temperature device includes a cover (5), an electric push rod (6), and a sealing strip (7). The cover (5) is rotatably mounted on the loading plate (1). The cover (5) and the loading plate (1) are equipped with an electric push rod (6). The sealing strip (7) is fixed on the mounting plate (4). The cover (5) is made of transparent material. The cover (5) is located above the reaction device. One end of the cover (5) is provided with an air inlet (501), and the other end is provided with an air outlet (502). The air inlet (501) is connected to an external air pump and heating device through a conduit. The air outlet (502) is connected to an exhaust device through a conduit. The cover (5) is also provided with a temperature sensor (503).

10. The single-well access enzyme-linked immunosorbent assay (ELISA) device as described in claim 9, characterized in that, The sealing strip (7) has a raised structure on the outside with one end higher than the other, and a ring-shaped raised structure on the inside. The two raised structures form a groove structure that fits the size of the cover (5).

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