Absorbable hernia patch with biological activity and preparation method

Abstract

The invention discloses an absorbable hernia patch with biological activity and a preparation method, the absorbable hernia patch comprises a patch, the thickness of the patch is 1mm, a coaxial electrostatic spinning technology is adopted to prepare fibers with a core-shell structure, the fibers are prepared by compounding poly (p-dioxanone) and poly (L-lactic acid), the core-shell structure comprises an outer layer material and an inner layer material, and the inner layer material is a porous material. Naringenin is loaded in the patch; controllable degradation and mechanical support are achieved, poly (p-dioxanone) and poly (L-lactic acid) are adopted as substrate materials, shell-core structure fibers are formed through coaxial electrostatic spinning, tissue repair is promoted through drug functionalization design, naringenin with biological activity is loaded in the patch, the effects of resisting inflammation and promoting angiogenesis and collagen deposition are achieved, and the anti-inflammatory effect is achieved. The patch is complete in fiber morphology, the tensile strength and the elongation at break both reach the level required by abdominal wall repair, the high quality residual rate is kept in the degradation process, and the naringenin can achieve slow release and continuously play a role.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a bioactive absorbable hernia patch and its preparation method. Background Technology

[0002] Abdominal wall hernia is a common clinical surgical condition. Hernia repair with implanted mesh has gradually become the mainstream treatment for abdominal wall hernias and is widely considered an important means of reducing recurrence rates and restoring the integrity of the abdominal wall. An ideal hernia mesh should possess appropriate strength and flexibility, providing effective mechanical support while promoting the remodeling and regeneration of host tissues, and avoiding complications such as tissue stiffness, contraction, and fibrotic scarring caused by long-term retention in the body.

[0003] Existing hernia patches are mainly divided into synthetic and biological types. Synthetic patches can be further divided into non-absorbable and absorbable patches. Non-absorbable patches can maintain mechanical support for a long time, but are prone to causing chronic foreign body reactions and infections. Absorbable patches are made from medical polymer materials and can degrade in the human body, but lack biological activity, and degradation byproducts often induce inflammation. Repair mainly relies on the formation of a fibrous capsule, leading to insufficient tissue fusion. Biological patches are derived from natural matrices and can promote tissue repair through cell migration and angiogenesis, but they degrade too quickly, often leading to recurrence. Existing patches mainly have the following shortcomings: synthetic absorbable patches have poor tissue repair quality; synthetic non-absorbable patches remain in the body for a long time, easily causing adverse complications; and biological patches degrade too quickly. Therefore, there is an urgent need to develop a bioactive absorbable hernia patch. Therefore, this invention proposes a bioactive absorbable hernia patch and its preparation method to solve the problems existing in the prior art. Summary of the Invention

[0004] To address the aforementioned problems, the present invention aims to provide a bioactive absorbable hernia patch and its preparation method. This bioactive absorbable hernia patch and its preparation method utilize controlled degradation and mechanical support: using poly(p-dioxanone) and polylactic acid (PLA) as base materials, a core-shell structure fiber is formed through coaxial electrospinning. This ensures both high strength and flexibility in the initial implantation stage while achieving slow and controlled degradation, meeting the mechanical requirements for long-term abdominal wall repair and avoiding the problems of rapid degradation in biological patches or long-term retention in non-absorbable patches. Drug functionalization promotes tissue repair: The patch is loaded with bioactive naringenin, achieving anti-inflammatory, angiogenesis-promoting, and collagen deposition-enhancing effects, overcoming the shortcomings of traditional synthetic patches that lack bioactivity and have insufficient repair quality, thereby significantly promoting tissue remodeling and regeneration. Excellent physicochemical and biological properties: The patch fibers maintain their integrity, with tensile strength and elongation at break reaching the levels required for abdominal wall repair, maintaining a high residual quality during degradation; naringenin can achieve sustained release, exerting a continuous effect and avoiding insufficient efficacy or side effects caused by short-term burst release.

[0005] To achieve the objectives of this invention, the following technical solution is provided: a bioactive absorbable hernia patch and its preparation method, comprising a patch with a thickness of 1 mm, wherein fibers with a core-shell structure are prepared by coaxial electrospinning technology, the fibers are prepared by composite of poly(p-dioxanone) and polylactic acid (L-l), the core-shell structure includes an outer layer material and an inner layer material, the patch is loaded with naringenin, the naringenin loading amount accounts for 1% of the mass-volume ratio of the solution, and the degradation cycle of the patch in vivo is 12 weeks.

[0006] A further improvement is that the shell-core structure adopts one of the following four configuration methods:

[0007] (1) The outer layer is made of polylactic acid (PLA) and the inner layer is made of polydioxanone (PDX). Naringenin is loaded only on the inner layer.

[0008] (2) The outer layer is made of polylactic acid (PLA) and the inner layer is made of polydioxanone (PDX). Both the outer and inner layers are loaded with naringenin.

[0009] (3) The outer layer is polydioxanone material, the inner layer is polylactic acid material, and naringenin is loaded only in the inner layer;

[0010] (4) The outer layer is polydioxanone material, the inner layer is polylactic acid material, and both the inner and outer layers are loaded with naringenin.

[0011] The further improvement lies in the fact that the diameter of the electrospun fiber is 1.25±0.37μm, the porosity is 31.02%±1.17%, and the water contact angle is 119.33°±3.00°.

[0012] The further improvement lies in the fact that the tensile strength of the patch is 36.47±2.40 N / cm and the elongation at break is 287.98%±51.67%.

[0013] A further improvement is that the naringenin is released slowly through the fibrous pores, with a release rate of 90.82% ± 0.67% within 19 days.

[0014] A method for preparing a bioactive absorbable hernia patch, characterized by comprising the following steps:

[0015] Step 1: Prepare a polydioxanone solution. Dissolve polydioxanone particles in a mixed solvent of dichloromethane / hexafluoroisopropanol to prepare a polydioxanone solution with a mass-volume ratio of 10% and a solvent volume ratio of 7:3.

[0016] Step 2: Prepare a polylactic acid (PLA) solution by dissolving PLA particles in a mixed solvent of dichloromethane and hexafluoroisopropanol to prepare a PLA solution with a mass-volume ratio of 8% and a solvent-volume ratio of 3:7.

[0017] Step 3: Loading naringenin. Add naringenin powder to a polydioxanone solution and / or a polylactic acid solution to prepare a drug loading solution with a mass-volume ratio of 1%.

[0018] Step 4: Solution treatment. Stir the prepared solution at 25°C and 300 rpm for 10 hours, and let it stand for 1 hour to remove bubbles.

[0019] Step 5: Coaxial electrospinning is carried out under the conditions of 10kV voltage, 2mL / h injection rate, 30%-40% humidity and 14cm receiving distance to produce an absorbable hernia patch.

[0020] Further improvements are made by using 17G and 20G needles for electrospinning, which produces patches that promote fibroblast proliferation and migration, and effectively promote the repair and regeneration of abdominal wall defects.

[0021] The beneficial effects of this invention are: controllable degradation and mechanical support: using polydioxanone and polylactic acid as base materials, and forming core-shell structure fibers through coaxial electrospinning, it can ensure high strength and flexibility in the early stage of implantation, and achieve slow and controllable degradation, meeting the mechanical requirements of long-term abdominal wall repair, and avoiding the problems of biological patches degrading too quickly or non-absorbable patches remaining for a long time.

[0022] Drug functionalization promotes tissue repair: Loading bioactive naringenin into patches achieves anti-inflammatory, angiogenesis-promoting, and collagen deposition-enhancing effects, improving the shortcomings of traditional synthetic patches that lack bioactivity and have insufficient repair quality, thereby significantly promoting tissue remodeling and regeneration.

[0023] Excellent physicochemical and biological properties: The patch fibers are intact, and the tensile strength and elongation at break are at the level required for abdominal wall repair. It maintains a high quality retention rate during degradation. Naringen can achieve sustained release and exert its effect continuously, avoiding insufficient efficacy or side effects caused by short-term burst release. Attached Figure Description

[0024] Figure 1 The inner layer of the present invention is a shell-core structure fiber loaded with naringenin (inner layer poly(p-dioxanone), outer layer poly(L-lactic acid)).

[0025] Figure 2 The present invention is a core-shell structure fiber with naringenin loaded in both the inner and outer layers (inner layer poly(p-dioxanone), outer layer poly(L-lactic acid)).

[0026] Figure 3 The inner layer of the present invention is a shell-core structure fiber loaded with naringenin (inner layer: L-polylactic acid, outer layer: poly(p-dioxanone)).

[0027] Figure 4 The present invention is a core-shell structure fiber with naringenin loaded in both the inner and outer layers (inner layer: L-polylactic acid, outer layer: poly(p-dioxanone)).

[0028] Figure 5 This is a scanning electron microscope image of the electrospun fibers of the present invention;

[0029] Figure 6 This is the in vitro drug release curve of the present invention;

[0030] Figure 7 Postoperative conditions in animal experiments of the present invention

[0031] Figure 8 This is a flowchart of the steps of the present invention. Detailed Implementation

[0032] To enhance understanding of the present invention, the present invention will be further described in detail below with reference to embodiments. These embodiments are only used to explain the present invention and do not constitute a limitation on the scope of protection of the present invention.

[0033] Example 1

[0034] according to Figure 1 As shown, this invention provides a bioactive absorbable hernia patch and its preparation method, with a thickness of approximately 1 mm. The patch is composed of electrospun fibers with a core-shell structure, the outer layer of which is L-polylactic acid, and the inner layer is poly(p-dioxanone) material, with naringenin loaded in the inner layer. This core-shell structure allows for slow and controllable degradation of the patch in vivo while providing sufficient mechanical support; through the drug functionalization design of naringenin, it can effectively promote tissue repair and regeneration.

[0035] Bioactive absorbable hernia patches were prepared according to the following method:

[0036] Step 1: Prepare a polydioxanone solution: At room temperature, weigh a certain mass of polydioxanone particles and dissolve them in a mixed solvent of dichloromethane / hexafluoroisopropanol (volume ratio 7:3) to prepare a polydioxanone solution with a mass-volume ratio (w / v) of 10%.

[0037] Step 2, loading drug into polydioxanone solution: At room temperature, weigh a certain amount of naringenin powder and add it to a 10% polydioxanone solution to prepare a 1% naringenin / polydioxanone solution by mass-volume ratio.

[0038] Step 3: Prepare a polylactic acid (PLA) solution: At room temperature, weigh a certain amount of PLA particles and dissolve them in a mixed solvent of dichloromethane / hexafluoroisopropanol (volume ratio 3:7) to prepare a PLA solution with a mass-volume ratio of 8%.

[0039] Step 4, Solution raw material processing: The prepared solution is sealed and placed on a constant temperature magnetic stirrer (temperature 25℃, speed 300 rpm) and stirred for 10 h. After standing for 1 h to remove bubbles, clear, uniform and stable spinning solutions are obtained, namely poly(p-dioxanone) / naringenin solution and poly(L-lactic acid) solution.

[0040] Step 5, coaxial electrospinning: Under the conditions of 10 kV voltage, infusion rate of 2 mL / h, air humidity of 30%-40%, and receiving distance of 14 cm, 17 G and 20 G needles were used to electrospin to prepare bioactive absorbable hernia patches.

[0041] Example 2

[0042] according to Figure 2 As shown, this invention provides a bioactive absorbable hernia patch and its preparation method, with a thickness of approximately 1 mm. The patch is composed of electrospun fibers with a core-shell structure. The outer layer of the fiber is made of L-polylactic acid, and naringenin is loaded in the outer layer. The inner layer is made of poly(p-dioxanone), and naringenin is also loaded in the inner layer. This core-shell structure allows for slow and controllable degradation of the patch in vivo while providing sufficient mechanical support. Through the drug functionalization design of naringenin, tissue repair and regeneration can be effectively promoted.

[0043] Bioactive absorbable hernia patches were prepared according to the following method:

[0044] Step 1: Prepare a polydioxanone solution: At room temperature, weigh a certain mass of polydioxanone particles and dissolve them in a mixed solvent of dichloromethane / hexafluoroisopropanol (volume ratio 7:3) to prepare a polydioxanone solution with a mass-volume ratio of 10%.

[0045] Step 2, loading drug into polydioxanone solution: At room temperature, weigh a certain amount of naringenin powder and add it to a 10% polydioxanone solution to prepare a 1% (by mass / volume) naringenin (polydioxanone) solution.

[0046] Step 3: Prepare a polylactic acid (PLA) solution: At room temperature, weigh a certain amount of PLA particles and dissolve them in a mixed solvent of dichloromethane / hexafluoroisopropanol (volume ratio 3:7) to prepare a PLA solution with a mass-volume ratio of 8%.

[0047] Step 4: Drug loading with polylactic acid (PLA) solution: At room temperature, weigh a certain amount of naringenin powder and add it to a 10% PLA solution to prepare a 1% (by mass / volume) naringenin (PLA) solution.

[0048] Step 5, Solution raw material processing: The prepared solution is sealed and placed on a constant temperature magnetic stirrer (temperature 25℃, speed 300 rpm) and stirred for 10 h. After standing for 1 h to remove bubbles, clear, uniform and stable spinning solutions are obtained, namely poly(p-dioxanone) / naringenin solution and poly(L-lactic acid) / naringenin solution.

[0049] Step 6: Coaxial electrospinning: Under the conditions of 10 kV voltage, infusion rate of 2 mL / h, air humidity of 30%-40%, and receiving distance of 14 cm, 17 G and 20 G needles were used to electrospin to prepare bioactive absorbable hernia patches.

[0050] Example 3

[0051] according to Figure 3 As shown, this invention provides a bioactive absorbable hernia patch and its preparation method, with a thickness of approximately 1 mm. The patch is composed of electrospun fibers with a core-shell structure. The outer layer of the fiber is made of poly(p-dioxanone) ketone, and the inner layer is made of polylactic acid (PLA), with naringenin loaded in the inner layer. This core-shell structure allows for slow and controllable degradation of the patch in vivo while providing sufficient mechanical support. Through the drug functionalization design of naringenin, it can effectively promote tissue repair and regeneration.

[0052] Bioactive absorbable hernia patches were prepared according to the following method:

[0053] Step 1: Prepare a polydioxanone solution: At room temperature, weigh a certain mass of polydioxanone particles and dissolve them in a mixed solvent of dichloromethane / hexafluoroisopropanol (volume ratio 7:3) to prepare a polydioxanone solution with a mass-volume ratio of 10%.

[0054] Step 2: Prepare a polylactic acid (PLA) solution: At room temperature, weigh a certain amount of PLA particles and dissolve them in a mixed solvent of dichloromethane / hexafluoroisopropanol (volume ratio 3:7) to prepare a PLA solution with a mass-volume ratio of 8%.

[0055] Step 3: Drug loading with polylactic acid (PLA) solution: At room temperature, weigh a certain amount of naringenin powder and add it to an 8% PLA solution to prepare a 1% naringenin (PLA) solution by mass-volume ratio.

[0056] Step 4, Solution raw material processing: The prepared solution is sealed and placed on a constant temperature magnetic stirrer (temperature 25℃, speed 300 rpm) and stirred for 10 h. After standing for 1 h to remove bubbles, clear, uniform and stable spinning solutions are obtained, namely poly(p-dioxanone) solution and poly(l-lactic acid) / naringenin solution.

[0057] Step 5, coaxial electrospinning: Under the conditions of 10 kV voltage, infusion rate of 2 mL / h, air humidity of 30%-40%, and receiving distance of 14 cm, 17 G and 20 G needles were used to electrospin to prepare bioactive absorbable hernia patches.

[0058] Example 4

[0059] according to Figure 4 As shown, this invention provides a bioactive absorbable hernia patch and its preparation method, with a thickness of approximately 1 mm. The patch is composed of electrospun fibers with a core-shell structure. The outer layer of the fiber is made of poly(p-dioxanone) and the inner layer is made of polylactic acid (PLA), with naringenin loaded in both the inner and outer layers. This core-shell structure allows for slow and controllable degradation of the patch in vivo while providing sufficient mechanical support. Through the drug functionalization design of naringenin, it can effectively promote tissue repair and regeneration.

[0060] Bioactive absorbable hernia patches were prepared according to the following method:

[0061] Step 1: Prepare a polydioxanone solution: At room temperature, weigh a certain mass of polydioxanone particles and dissolve them in a mixed solvent of dichloromethane / hexafluoroisopropanol (volume ratio 7:3) to prepare a polydioxanone solution with a mass-volume ratio of 10%.

[0062] Step 2, loading drug into polydioxanone solution: At room temperature, weigh a certain amount of naringenin powder and add it to an 8% polydioxanone solution to prepare a 1% (by mass / volume) naringenin (polydioxanone) solution.

[0063] Step 3: Prepare a polylactic acid (PLA) solution: At room temperature, weigh a certain amount of PLA particles and dissolve them in a mixed solvent of dichloromethane / hexafluoroisopropanol (volume ratio 3:7) to prepare a PLA solution with a mass-volume ratio of 8%.

[0064] Step 4: Drug loading with polylactic acid (PLA) solution: At room temperature, weigh a certain amount of naringenin powder and add it to an 8% PLA solution to prepare a 1% naringenin / PLA solution by mass / volume ratio.

[0065] Step 5, Solution raw material processing: The prepared solution is sealed and placed on a constant temperature magnetic stirrer (temperature 25℃, speed 300 rpm) and stirred for 10 h. After standing for 1 h to remove bubbles, clear, uniform and stable spinning solutions are obtained, namely poly(p-dioxanone) / naringenin solution and poly(L-lactic acid) / naringenin solution.

[0066] Step 6: Coaxial electrospinning: Under the conditions of 10 kV voltage, infusion rate of 2 mL / h, air humidity of 30%-40%, and receiving distance of 14 cm, 17 G and 20 G needles were used to electrospin to prepare bioactive absorbable hernia patches.

[0067] Example 1 demonstrated the best performance in laboratory studies, and the specific experimental data are as follows:

[0068] This invention validates the physicochemical properties, in vitro biological properties, and in vivo safety of the patch in animals, and conducts a preliminary study on the mechanism by which naringenin promotes abdominal wall repair.

[0069] Physicochemical performance testing:

[0070] The surface morphology of the patch was observed using field emission scanning electron microscopy, and the fiber diameter and porosity were measured using ImageJ software. The results showed that the electrospun fibers in the patch had a diameter of 1.25 ± 0.37 μm and a porosity of 31.02% ± 1.17%.

[0071] The surface wettability of the patch was tested using a contact angle meter via the droplet method. For each test, a 5 μL droplet was placed on the material surface, and the contact angle was recorded after 10 seconds of incubation. The results showed that the water contact angle was 119.33° ± 3.00°.

[0072] Referring to ASTM D5035-2011 standard, patch specimens were cut into 30 mm × 50 mm pieces, with at least three parallel specimens per group. These were fixed at both ends using a computer-controlled multi-functional materials testing machine for fixed uniaxial tensile testing. The test was terminated upon specimen fracture. The gauge length was 30 mm, and the tensile speed was 10 mm / min. The results showed that the tensile strength of the patch was 36.47 ± 2.40 N / cm, and the corresponding elongation at break was 287.98% ± 51.67%, fully meeting the mechanical strength required for the human abdominal wall and demonstrating its potential for implantation in the human body for abdominal wall defect repair and regeneration.

[0073] In vitro biological performance experiments:

[0074] Bioactive absorbable hernia patches were ultrasonically cleaned three times in deionized water and then dried at 37°C to constant weight. 200 mg of sample was accurately weighed, and three parallel samples were prepared. 50 mL of PBS was added to each sample, and the samples were incubated in a shaker at 37°C at 100 rpm. Every two weeks, the patches were removed, cleaned three times with deionized water, and dried at 37°C to constant weight. The remaining patch mass was accurately weighed and recorded. The results showed that the core-shell structure of the patch effectively delayed patch degradation and drug release. Naringenin slowly and continuously reached its maximum release of 90.82% ± 0.67% within 19 days, and the residual mass of the patch was still 84.50% ± 3.14% at week 12.

[0075] The bioactive absorbable hernia patch was used in cell proliferation experiments with mouse fibroblast L929 cells. The patch was cut into 15 mm diameter discs, and three replicates were prepared for each time point. The discs were ultrasonically washed three times in deionized water, dried at 37°C to constant weight, and sterilized by UV irradiation for 5 h before being placed in 24-well plates. L929 fibroblasts were cultured in DMEM medium containing 10% fetal bovine serum and 1% penicillin antibiotics in a 5% CO2 incubator (37°C). The seeding density of the 24-well plates was 10,000 cells / well. At 24 h, 48 h, 72 h, and 96 h post-seeding, the old medium was aspirated from each well, and fresh medium containing 10% CCK-8 reagent was added before further incubation for 3 h. After culture, the 24-well plates were removed, and the cell suspensions were transferred to 96-well plates. The OD value at 450 nm was measured using a microplate reader, and cell viability was calculated. The results showed that the patch promoted fibroblast proliferation and migration.

[0076] Animal experiments:

[0077] To verify the in vivo repair effect of a novel bioactive absorbable hernia patch, a healthy rat model of abdominal wall defect was established. First, the patch sample was cut to 3 cm × 3 cm, ultrasonically cleaned, dried, and sterilized under ultraviolet light. Five rats were used in the experiment. Preoperatively, the rats were weighed, and the abdomen was prepared and disinfected. After anesthesia with 1% sodium pentobarbital, a longitudinal incision of approximately 6 cm was made along the midline below the xiphoid process to create a 1.5 cm × 1.5 cm full-thickness abdominal wall defect. The patch was used to bridge and repair the defect and fixed with 3-0 PGA absorbable sutures. After hemostasis, the skin was sutured. Postoperatively, the rats were routinely fed, and their activity level and the presence of infection, seroma, incision dehiscence, abdominal wall bulging, or death at the surgical site were observed. Rats were sacrificed on days 7, 14, and 28 postoperatively, and specimens from the repaired area were collected. The degree of abdominal adhesion after patch implantation was scored and evaluated based on the adhesion area and strength. The results showed that the patch effectively promoted the repair and regeneration of abdominal wall defects in rats, with the newly formed collagen tissue arranged in a regular and orderly manner. Immunohistochemical results indicated that the abdominal wall repair and regeneration effect of the patch may be related to the inhibition of MMP2 and IL-6 expression and the promotion of VEGF expression by naringenin.

[0078] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A bioactive absorbable hernia patch, characterized in that, The invention includes a patch with a thickness of approximately 1 mm, which is prepared using coaxial electrospinning technology to form a core-shell structure. The fiber is prepared by combining poly(p-dioxanone) and polylactic acid (PLA). The core-shell structure includes an outer layer material and an inner layer material. The patch is loaded with naringenin, and the naringenin loading accounts for 1% of the mass-volume ratio of the solution. The degradation cycle of the patch in vivo is 12 weeks.

2. The bioactive absorbable hernia patch according to claim 1, characterized in that: The shell-core structure adopts one of the following four configuration methods: (1) The outer layer is made of polylactic acid (PLA) and the inner layer is made of polydioxanone (PDX). Naringenin is loaded only on the inner layer. (2) The outer layer is made of polylactic acid (PLA) and the inner layer is made of polydioxanone (PDX). Both the outer and inner layers are loaded with naringenin. (3) The outer layer is polydioxanone material, the inner layer is polylactic acid material, and naringenin is loaded only in the inner layer; (4) The outer layer is polydioxanone material, the inner layer is polylactic acid material, and both the inner and outer layers are loaded with naringenin.

3. The bioactive absorbable hernia patch according to claim 1, characterized in that: The electrospun fiber has a diameter of 1.25±0.37μm, a porosity of 31.02%±1.17%, and a water contact angle of 119.33°±3.00°.

4. The bioactive absorbable hernia patch according to claim 1, characterized in that: The tensile strength of the patch is 36.47±2.40 N / cm, and the elongation at break is 287.98%±51.67%.

5. The bioactive absorbable hernia patch according to claim 1, characterized in that: The naringenin is released slowly through the fibrous pores, with a release rate of 90.82% ± 0.67% over 19 days.

6. A method for preparing a bioactive absorbable hernia patch according to any one of claims 1-5, characterized in that, Includes the following steps: Step 1: Prepare a polydioxanone solution. Dissolve polydioxanone particles in a mixed solvent of dichloromethane / hexafluoroisopropanol to prepare a polydioxanone solution with a mass-volume ratio of 10% and a solvent volume ratio of 7:

3. Step 2: Prepare a polylactic acid (PLA) solution by dissolving PLA particles in a mixed solvent of dichloromethane and hexafluoroisopropanol to prepare a PLA solution with a mass-volume ratio of 8% and a solvent-volume ratio of 3:

7. Step 3: Loading naringenin. Add naringenin powder to a polydioxanone solution and / or a polylactic acid solution to prepare a drug loading solution with a mass-volume ratio of 1%. Step 4: Solution treatment. Stir the prepared solution at 25°C and 300 rpm for 10 hours, and let it stand for 1 hour to remove bubbles. Step 5: Coaxial electrospinning is carried out under the conditions of 10kV voltage, 2mL / h injection rate, 30%-40% humidity and 14cm receiving distance to produce an absorbable hernia patch.

7. The method for preparing a bioactive absorbable hernia patch according to claim 6, characterized in that: The electrospinning process uses 17G and 20G needles, and the resulting patch promotes fibroblast proliferation and migration, and effectively promotes the repair and regeneration of abdominal wall defects.

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