Large-range automatic accurate control pipette liquid preparation method

Abstract

The invention discloses a large-range automatic accurate control pipette liquid preparation method, and belongs to the technical field of pipettes. According to the invention, a single device depends on a double-peristaltic pump combined mode, the wide-range liquid preparation requirement is met, a plurality of pipettes do not need to be matched, and the cost and the space are saved; through integer allocation and margin high-precision iteration, the minimum precision reaches 0.01 [mu] L, and the non-integer volume requirement is accurately met; an automatic mode, a high-precision mode and a manual mode are provided, different use scenes are adapted, and the operation threshold is lowered; manual contact is reduced, liquid pollution and volatilization risks are reduced, and the device is suitable for accurate liquid preparation in multiple fields.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pipette, in particular to a pipette liquid preparation method with wide range automatic and precise control. BACKGROUND

[0002] As a core tool in the field of chemical analysis, the liquid preparation precision and efficiency of pipette directly affect the reliability of experimental results, the accuracy of detection data and the stability of production process. In practical application, pipette liquid preparation needs to meet the liquid transfer requirements of different volumes (from microliters to milliliters).

[0003] The existing pipette liquid preparation generally needs multiple range pipettes to work together, which needs to take liquid multiple times and is mostly in manual mode, which is time-consuming and laborious to operate and has low work efficiency. Therefore, a pipette liquid preparation method with wide range automatic and precise control is proposed. SUMMARY

[0004] The technical problem to be solved by the present application is how to solve the problem that the existing pipette liquid preparation generally needs multiple range pipettes to work together, which needs to take liquid multiple times and is mostly in manual mode, which is time-consuming and laborious to operate and has low work efficiency, and to provide a pipette liquid preparation method with wide range automatic and precise control.

[0005] The present application solves the above technical problems by the following technical solutions, and comprises the following steps:

[0006] S1: obtaining the liquid volume to be prepared as a target value;

[0007] S2: selecting a working mode, the working mode including an automatic mode, a high precision mode and a manual mode;

[0008] S3: when the working mode is selected as the automatic mode, the target value is taken down multiple times to obtain corresponding integer values, the rotation amount of each pump is distributed according to the single circle liquid taking amount of the two peristaltic pumps according to the integer value of each time, until the residual amount of the target value is zero, and the liquid taking is completed;

[0009] S4: when the working mode is selected as the high precision mode, the minimum precision is calculated directly according to the target value, the rotation amount of each pump is distributed according to the single circle liquid taking amount of the two peristaltic pumps according to the minimum precision amount, and the liquid taking is completed after the rotation is completed;

[0010] S5: when the working mode is selected as the manual mode, the rotation circle number and direction of the two peristaltic pumps are directly input, the two peristaltic pumps rotate according to the input rotation circle number and direction, and the liquid taking is completed after the rotation is completed.

[0011] Further, the pipette comprises two peristaltic pumps and an air cavity, the two peristaltic pumps are communicated with the air cavity through a hose respectively, the air cavity is provided with a liquid inlet below, the two peristaltic pumps are controlled to rotate by a motor respectively, and the liquid inlet is provided with a suction head.

[0012] Further, in the step S1, the target value is obtained by direct manual input or voice input.

[0013] Further, in the step S3, the specific processing process is as follows:

[0014] S31: first, the target value is rounded down to obtain an integer value, and the rotation amount of each pump is distributed according to the liquid amount of a single circle of the two peristaltic pumps according to the integer value;

[0015] S32: continue to round down the remainder of the target value to obtain a tenth value, and continue to repeatedly distribute the rotation amount of each pump according to the liquid amount of a single circle of the two peristaltic pumps according to the tenth value;

[0016] S33: continue to round down the remainder of the target value to obtain a hundredth value, and continue to repeatedly distribute the rotation amount of each pump according to the liquid amount of a single circle of the two peristaltic pumps according to the hundredth value;

[0017] S34: until the remainder of the target value is zero, the liquid is completed.

[0018] Further, in the steps S31 and S32, when the sizes of the two peristaltic pumps are different, after obtaining the corresponding integer value, if the integer value is less than the volume of a single circle of the large peristaltic pump, the volume distributed to the large peristaltic pump and the small peristaltic pump is 1 / 2, and if the integer value is odd, the large peristaltic pump occupies 1 μL more; if it is greater than or equal to the volume of a single circle of the large peristaltic pump, the large peristaltic pump is preferentially distributed, and the remaining volume is completed by the small peristaltic pump.

[0019] Further, in the step S33, after obtaining the hundredth value, the minimum precision is extracted, the number of minimum precisions is calculated, one motor is controlled to intake air and the other motor is controlled to exhaust air, at this time, the large peristaltic pump rotates m times to intake air, and the small peristaltic pump needs to rotate n times to exhaust air, then one extraction is completed, and the liquid extraction is completed after repeating multiple times.

[0020] Further, in the step S4, after calculating the number of minimum precisions, one motor is controlled to intake air and the other motor is controlled to exhaust air, at this time, the large peristaltic pump rotates m times to intake air, and the small peristaltic pump needs to rotate n times to exhaust air, then one extraction is completed, and the liquid extraction work is completed after repeating multiple times.

[0021] Compared with existing technologies, this invention has the following advantages: This wide-range automatic and precise control pipette dispensing method, relying on a dual peristaltic pump combination mode, covers a wide range of dispensing needs without the need for multiple pipettes, saving costs and space; through integer allocation plus high-precision iteration with residual volume, the minimum accuracy reaches 0.01μL, accurately adapting to non-integer volume requirements; it provides three modes: automatic, high-precision, and manual, adapting to different usage scenarios and lowering the operation threshold; it reduces manual contact, reduces the risk of liquid contamination and volatilization, and is suitable for precise dispensing in multiple fields. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the hardware design structure in an embodiment of the present invention.

[0023] Figure 2 This is a schematic flowchart of a pipette liquid preparation method with large-scale automatic and precise control in an embodiment of the present invention;

[0024] In the diagram: 1. Peristaltic pump; 2. Hose; 3. Air chamber. Detailed Implementation

[0025] The embodiments of the present invention are described in detail below. These embodiments are implemented based on the technical solution of the present invention, and provide detailed implementation methods and specific operation processes. However, the scope of protection of the present invention is not limited to the following embodiments.

[0026] Example 1

[0027] like Figure 1 As shown, this embodiment provides a technical solution: a pipette structure, whose hardware design mainly includes two peristaltic pumps and an air chamber. When liquid needs to be drawn, the rotation speed of the two peristaltic pumps is controlled by an algorithm to precisely control the amount of air expelled each time. During liquid drawing, there are multiple combination modes depending on the size of the pumps. For example, if the two pumps are of the same size, one rotation will extract 10 μL of the target liquid. If the target value is 10.1 μL, the rotation speed and direction of the two pumps are adjusted, with one pump expelling air and the other inhaling air. One pump rotates forward one revolution (expelling air), while the other rotates in the opposite direction 0.99 revolutions (inhaling air), entering the precise extraction mode, extracting 0.1 μL of the target liquid each time until the target value is reached. By controlling the rotation speed of the two pumps, the minimum extraction value can be adjusted. Compared to current pipettes that require manual operation and multiple pipettes with different volume ranges, this method only requires a single pipette to solve the problem of different volume ranges, and it offers high precision and automatic control.

[0028] like Figure 2As shown, this embodiment also provides a technical solution: a large-range automatic and precise control method for pipette liquid preparation. First, the target value is obtained (generally by direct input or voice input). The target value is then decomposed. Taking 10.1 μL as an example, it is first rounded down to 10 μL. At this time, the motor is controlled to make each of the two pumps rotate half a turn, thus drawing 10 μL of liquid. Then, a high-precision mode is adopted, and the number of pump rotations and repetitions are obtained by dividing the margin value by the minimum precision value. At this time, one pump motor is controlled to rotate forward 1 turn, and the other pump rotates in the reverse direction 0.99 turns, repeating once to obtain the final target value. If the margin is 0.5, then it is repeated 5 times.

[0029] Example 2

[0030] In this embodiment, it is assumed that the two existing peristaltic pumps have an accuracy of 0.01 μL, and the pumps can draw 10 μL and 5 μL of liquid per revolution, respectively. Now, it is necessary to draw 8.88 μL of liquid, 9.88 μL of liquid, and 11.0 μL of liquid.

[0031] 1: The pipetting volumes obtained were 8.88 μL, 9.88 μL, and 11 μL.

[0032] 2: Select one of the three calculation modes, assuming it is automatic mode (the principle of automatic mode is that both pumps should be used, while manual mode can be used if only one pump is used).

[0033] 2-1: First, round down the target value of 8.88 (this rounding doesn't mean taking the exact integer, but rather rounding down each place value; for 8.88, this means rounding down three times: 8, 0.8, and 0.08). This gives a result of 8 μL. Since this is an even number, it's allocated as 4 μL and 4 μL respectively. Similarly, if 9.88 μL is rounded down to 9 μL, it will be allocated as 5 μL and 4 μL. If it's 11 μL, it will be allocated as 10 μL and 1 μL. The allocation rule is based on the following: if the liquid volume is less than the volume of one revolution of the large pump, both the large and small pumps receive half the volume. If the volume is odd, the large pump receives an extra 1 μL. If the volume is greater than or equal to the volume of one revolution of the large pump, the large pump receives the first portion, and the remaining volume is handled by the small pump (if the pump volumes are different).

[0034] 2-2: After the above operations, the integer part has been allocated. Now there is 0.88μL of liquid. We still round it to 0.8μL. After allocating to both sides, there are 0.4μL respectively. That is, we need to rotate 0.04 times and 0.08 times respectively. After reaching the above position, extract 0.01μL. At this time, according to the motor control, one motor is for air intake and the other is for air exhaust. At this time, the large pump rotates 0.999 times for air intake and the small pump needs to rotate 2 times for air exhaust. This completes one extraction. Repeat 8 times to complete the liquid extraction.

[0035] 2-3: After the above once rounding, the number of turns is allocated, the remaining amount is 0, and the calculation is completed.

[0036] 3: Select high-precision mode

[0037] Directly according to the minimum precision mode, that is, if it is 8.88 μL, first calculate the minimum precision, that is, 0.01 μL, at this time, calculate the rotation amount of two pumps according to 0.01 μL, that is, the small pump rotates 2 turns to exhaust, and the large pump rotates 0.999 turns to inhale, then calculate the number of times, and then the two pumps rotate according to the number of times.

[0038] 4: Manual mode

[0039] This part is mainly for the user to input the number of turns and direction of the two pumps, and the machine controls the pump to rotate according to the input number of turns and direction.

[0040] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limiting the present application, and those skilled in the art can make changes, modifications, replacements and modifications to the above embodiments within the scope of the present application.

Claims

1. A method for preparing solution by pipette with wide range automatic precision control, characterized in that, The method comprises the following steps: S1: obtaining the volume of the liquid to be taken as a target value; S2: selecting a working mode, the working mode comprising an automatic mode, a high-precision mode and a manual mode; S3: when the working mode is selected as the automatic mode, the target value is repeatedly rounded down to obtain corresponding rounding values, the rotation amount of each pump is allocated according to the rounding value of each time and the single-circle liquid taking amount of the two peristaltic pumps until the residual amount of the target value is zero, and the liquid taking is completed; S4: when the working mode is selected as the high-precision mode, the minimum precision is directly calculated according to the target value, the rotation amount of each pump is allocated according to the minimum precision amount and the single-circle liquid taking amount of the two peristaltic pumps, and the liquid taking is completed after the rotation is completed; S5: when the working mode is selected as the manual mode, the rotation circle number and direction of the two peristaltic pumps are directly input, the two peristaltic pumps rotate according to the input rotation circle number and direction, and the liquid taking is completed after the rotation is completed.

2. The method according to claim 1, wherein, The pipette comprises two peristaltic pumps and an air cavity, the two peristaltic pumps are communicated with the air cavity through a hose respectively, a liquid inlet is arranged below the air cavity, the two peristaltic pumps are controlled to rotate by a motor, and a suction head is arranged on the liquid inlet.

3. The method according to claim 1, wherein, In the step S1, the target value is obtained by direct manual input or voice input.

4. The method according to claim 2, wherein, In the step S3, the specific processing process is as follows: S31: the target value is first rounded down once, the rotation amount of each pump is allocated according to the integer rounding value and the single-circle liquid taking amount of the two peristaltic pumps; S32: the residual amount of the target value is continuously rounded down to obtain a tenth rounding value, the rotation amount of each pump is continuously repeatedly allocated according to the tenth rounding value and the single-circle liquid taking amount of the two peristaltic pumps; S33: the residual amount of the target value is continuously rounded down to obtain a hundredth rounding value, the rotation amount of each pump is continuously repeatedly allocated according to the hundredth rounding value and the single-circle liquid taking amount of the two peristaltic pumps; S34: until the residual amount of the target value is zero, the liquid taking is completed.

5. The method according to claim 4, wherein, In the steps S31 and S32, when the sizes of the two peristaltic pumps are different, after the corresponding rounding values are obtained, the allocation rule is that, if the rounding value is less than the volume of one rotation of the large peristaltic pump, the volume allocated to the large peristaltic pump and the small peristaltic pump is 1 / 2, if the rounding value is odd, the large peristaltic pump occupies 1 μL more, and if the rounding value is greater than or equal to the volume of one rotation of the large peristaltic pump, the large peristaltic pump is preferentially allocated, and the remaining volume is completed by the small peristaltic pump.

6. The method according to claim 5, wherein, In the step S33, after the hundredth rounding value is obtained, the minimum precision is extracted, the minimum precision amount is calculated, one motor is controlled to intake air and the other motor is controlled to exhaust air, at this time, the large peristaltic pump rotates m times to intake air, and the small peristaltic pump needs to rotate n times to exhaust air, then one extraction is completed, and the liquid extraction is completed after repeated extraction.

7. The method as claimed in claim 1, wherein the method is characterized by, In the step S4, after the minimum precision amount is calculated, one motor is controlled to intake air and the other motor is controlled to exhaust air, at this time, the large peristaltic pump rotates m times to intake air, and the small peristaltic pump needs to rotate n times to exhaust air, then one extraction is completed, and the liquid taking work is completed after repeated extraction.

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