Extraction and purification method of phytosphingosine synthesized by whole cell fermentation

This method, which synthesizes plant sphingosine through whole-cell fermentation, utilizes enzymatic hydrolysis and macroporous resin for impurity removal, followed by deep purification with acetonitrile. This solves the separation and purification challenges in bio-fermentation, achieving efficient and environmentally friendly preparation of high-purity sphingosine suitable for cosmetics and skincare products.

CN121574060BActive Publication Date: 2026-05-29云合(天津)生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
云合(天津)生物技术有限公司
Filing Date
2026-01-27
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

In existing technologies, the separation and purification of plant sphingosine through bio-fermentation is difficult, resulting in low economic efficiency and low cost of producing high-quality sphingosine. Chemical synthesis methods, on the other hand, are difficult, costly, and environmentally problematic.

Method used

A whole-cell fermentation method for the synthesis of plant sphingosine was developed, which includes steps such as enzymatic hydrolysis, ceramic membrane washing, hydrolysis, extraction, centrifugation, concentration, purification, filtration and crystallization. Lysozyme and macroporous resin were used for impurity removal, and acetonitrile was used for deep purification to obtain high-purity sphingosine.

Benefits of technology

The preparation of high-purity (95%) plant sphingosine was achieved with a high yield (greater than 90%), which reduced solvent costs, made it suitable for large-scale production, and met environmental protection requirements.

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Abstract

The application provides an extraction and purification method for synthesizing phytosphingosine through whole-cell fermentation, which comprises the following steps: performing enzymolysis, ceramic membrane permeation and concentration on a fermentation liquor to obtain a bacteria slurry liquor; adding a strong base to the bacteria slurry liquor and performing primary hydrolysis under heating to obtain a hydrolysis liquor; adjusting the pH value of the hydrolysis liquor to 7-8, and then performing extraction, centrifugation, concentration, purification, purification, filtration and drying to obtain high-purity N-acetyl phytosphingosine; adding the high-purity N-acetyl phytosphingosine into an ethanol aqueous solution, adding a strong base into the ethanol aqueous solution, and performing secondary hydrolysis under heating to obtain a phytosphingosine reaction liquor; and performing crystallization, recrystallization, centrifugation and drying on the phytosphingosine reaction liquor to obtain the phytosphingosine. The extraction and purification method releases the cell contents by using lysozyme, removes water-soluble impurities through a ceramic membrane, and retains tetraacetyl phytosphingosine phytosphingosine, and is efficient and fast.
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