Liposome composition wrapping acne-removing component as well as preparation method and application of liposome composition

Abstract

The invention relates to a lipidosome composition wrapping an acne removing component as well as a preparation method and application of the lipidosome composition. The composition comprises the following components in percentage by weight: 1-5% of colloidal sulfur, 0.1-1% of an African amomum kravanh seed extract, 0.1-1% of a white willow bark extract, 0.1-1% of a radix gentianae extract, 0.1-1% of a pueraria mirifica extract, 0.1-1% of a yeast / zinc fermentation product, 1-3% of melaleuca alternifolia leaf oil, 0.01-1% of rice chaff oil, 1-20% of an emulsifier, 1-10% of lipid, 40-60% of polyol, 0.01-1% of an antioxidant, 0.1-0.5% of a pH regulator and 0.01-0.5% of a preservative. And the balance of water. The composition not only can realize efficient acne removal, but also can gently care the skin.

Description

Technical Field

[0001] This invention relates to the technical field of acne-removing cosmetics, specifically to a liposome composition encapsulating acne-removing ingredients, its preparation method, and its application. Background Technology

[0002] Acne, commonly known as pimples, is an extremely common skin problem. It mainly stems from excessive sebum secretion, abnormal follicular keratinization, Propionibacterium acnes infection, and the resulting inflammatory response. Currently, the market is flooded with acne treatment products; however, these products generally have drawbacks, such as being highly irritating, having limited effectiveness, or relying on hormonal ingredients. This makes it difficult for them to meet consumers' expectations for gentle yet highly effective acne treatments.

[0003] Currently, ingredients such as salicylic acid and Melaleuca alternifolia leaf oil are widely used in acne treatment products. However, directly using these ingredients may irritate sensitive skin. In contrast, natural plant extracts, with their gentleness, safety, and diverse bioactivities, are gradually becoming an indispensable ingredient in acne cosmetics. Although many plant extracts are already used in acne treatment products, existing products still have certain limitations. For example, some products are not effective enough in treating acne, or are not suitable for sensitive skin. Therefore, there is an urgent market demand for developing a natural plant composition that can effectively remove acne while gently caring for the skin. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a liposome composition encapsulating acne-removing ingredients, its preparation method, and its application.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a liposome composition encapsulating acne-reducing ingredients, comprising the following components by weight percentage: 1-5% colloidal sulfur, 0.1-1% African cardamom seed extract, 0.1-1% white willow bark extract, 0.1-1% gentian root extract, 0.1-1% pueraria lobata root extract, 0.1-1% yeast / zinc fermentation product, 1-3% Melaleuca alternifolia leaf oil, 0.01-1% rice bran oil, 1-20% emulsifier, 1-10% lipid, 40-60% polyol, 0.01-1% antioxidant, 0.1-0.5% pH adjuster, and 0.01-0.5% preservative, with the balance being water.

[0006] This invention utilizes liposomes to encapsulate acne-fighting ingredients, reducing direct contact between components such as Melaleuca alternifolia leaf oil and the stratum corneum of the skin, thereby significantly reducing irritation. Simultaneously, encapsulation can mask some of their odor. Furthermore, encapsulating the acne-fighting ingredients within the liposome membrane allows for slow and controlled release, avoiding irritation from instantaneous high concentrations, while protecting their active ingredients and prolonging their duration of action.

[0007] The following are the main functions of the acne-fighting ingredients in the composition: Colloidal sulfur has antibacterial properties that inhibit the growth of Propionibacterium acnes, and also has anti-inflammatory effects and reduces sebum secretion.

[0008] Tea tree oil (Melaleuca alternifolia leaf oil) has antibacterial and anti-inflammatory properties, can tighten pores, and treat suppurating wounds. It can further enhance the inhibitory effect on Propionibacterium acnes.

[0009] Zinc ions in yeast / zinc fermentation products inhibit excessive sebum secretion, reduce oil blockage in hair follicles, and reduce the release of inflammatory factors, relieving acne redness and swelling. By utilizing the ability of brewer's yeast to accumulate zinc during its growth and metabolism, the anti-inflammatory, acne-removing, and oil-controlling effects are further enhanced.

[0010] African cardamom seed extract is rich in polyphenols and flavonoids, which can effectively inhibit inflammatory factors, enhance the skin barrier function, and alleviate skin redness.

[0011] The main active ingredient in white willow bark extract is salicin. As a precursor to salicylic acid, salicin is metabolized into salicylic acid after entering the human body, and plays a role in the skin by gently exfoliating, controlling sebum secretion, and anti-inflammation.

[0012] The main components of gentian root extract are gentiopicroside, gentiobiose and gentic acid. It has effects on atopic dermatitis and allergies, can inhibit irritation, play an anti-inflammatory role, promote the secretion of cholesterol in epidermal cells, and improve the condition of oily and rough skin.

[0013] Pueraria lobata extract possesses anti-inflammatory, antioxidant, and skin metabolism-regulating effects. It can inhibit the production of inflammatory factors, thereby reducing inflammation in acne and the areas surrounding pimples; in addition, it can promote skin metabolism and help repair damaged skin barrier function.

[0014] Preferably, the weight ratio of the African cardamom seed extract, white willow bark extract, gentian root extract and kudzu root extract is 1:(0.2-0.4):(0.5-1):1.

[0015] Preferably, the polyol is at least one selected from glycerol, 1,3-propanediol, 1,2-pentanediol, and hexanediol; the lipid is at least one selected from hydrogenated lecithin, soybean lecithin, phosphatidylcholine, distearyl phosphatidylcholine, and dipalmitoyl phosphatidylcholine; and the emulsifier is at least one selected from polyglycerol-10 oleate, polyglycerol-10 laurate, cetearyl alcohol, polysorbate-20, and PEG-40 hydrogenated castor oil.

[0016] Preferably, the antioxidant is tocopherol (vitamin E); the preservative is p-hydroxyacetophenone and ethylhexylglycerin; and the pH adjuster is hydroxyethylpiperazine ethanesulfonic acid.

[0017] In a second aspect, the present invention provides a method for preparing the liposome composition encapsulating acne-removing ingredients as described in the first aspect, comprising the following steps: S1. Alcohol phase preparation: Emulsifier, lipid, rice bran oil, antioxidant, polyol and preservative are mixed, heated and kept warm and stirred evenly to obtain alcohol phase solution; S2. Oil phase preparation: The oil from Melaleuca alternifolia leaves was used as the oil phase; S3. Aqueous phase preparation: Mix the pH adjuster, African cardamom seed extract, white willow bark extract, gentian root extract, kudzu root extract and yeast / zinc fermentation product, stir well to obtain an aqueous phase solution. S4. Add the oil phase dropwise to the alcohol phase solution, stir at high speed, then add the aqueous phase solution dropwise, and stir at high speed to form the primary emulsion. S5. Homogenize the colostrum using a microfluidic homogenizer to obtain liposomes, then add colloidal sulfur and stir to obtain the liposome composition encapsulating the acne-removing ingredients.

[0018] Preferably, in step S1, the stirring temperature is 50-60℃, the stirring speed is 500-1000 rpm, and the stirring time is 10-20 min.

[0019] Preferably, in step S3, the stirring temperature is 20-30℃, the stirring speed is 500-1000 rpm, and the stirring time is 10-20 min.

[0020] Preferably, in step S4, the high-speed stirring temperature is 50-60℃, the rotation speed is 1000-2000rpm, and the time is 10-20min.

[0021] Preferably, in step S5, the homogenization pressure is 50-250 MPa and the time is 5-15 min.

[0022] Thirdly, the present invention provides the application of the liposome composition encapsulating acne-removing ingredients in the first aspect in the preparation of acne-removing products.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention encapsulates the effective acne-fighting ingredients within liposomes, while also combining them with extracts of African cardamom seed, white willow bark, gentian root, and kudzu root. Through synergistic effects of antibacterial, anti-inflammatory, soothing, and repairing properties, it not only significantly enhances the acne-fighting efficacy but also effectively reduces the skin irritation caused by tea tree oil. The overall formula is gentle and unlikely to cause skin irritation, making it suitable for people with sensitive skin. Detailed Implementation

[0024] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0025] The sources of the raw materials used in the following examples and comparative examples are as follows: Colloidal sulfur: manufactured by Vantrus Functional Materials, trade name Sulfidal Colloidal SuleurCosmetic Grade; Melaleuca alternifolia leaf oil: Manufacturer is Shanghai Lishengshengte Enterprise Development Co., Ltd., product name is LS-416 tea tree oil; African cardamom seed extract: manufactured by IDBIO SAS, trade name PHYT'ANIM GRAINS OFPARADISE / BLG0-MNG-10; Gentian root extract: manufactured by Shanghai Jiayu Biotechnology Co., Ltd., traded under the name Decolorized Gentian Anti-irritant Factor (Butanediol); White Willow Bark Extract: Manufacturer: Active Concepts, LLC; Trade name: 10229-ABS WhiteWillow Bark Extract Powder; Pueraria lobata root extract: manufactured by BASF (China) Co., Ltd., trade name A00052INHIPASE; Hydroxyethylpiperazine ethane sulfonic acid: Manufacturer: UNIPROMA CO, LIMITED; Trade name: PromaCare ® HEPES; Yeast / Zinc Fermentation Products: Manufacturer: Angel Yeast Co., Ltd., Trade Name: Yeast Essence Z20 (N).

[0026] Unless otherwise specified, all other materials, reagents, etc. used in the examples and comparative examples are commercially available.

[0027] Example 1 A liposome composition encapsulating acne-fighting ingredients comprises the following components by weight percentage: 3% colloidal sulfur, 1% African cardamom seed extract, 0.3% white willow bark extract, 0.6% gentian root extract, 1% pueraria lobata root extract, 0.06% yeast / zinc fermentation product, 1.5% Melaleuca alternifolia leaf oil, 0.05% rice bran oil, 12% emulsifier, 8% lipid, 48% polyol, 0.02% antioxidant, 0.3% pH adjuster, and 0.1% preservative, with the balance being water; wherein the polyol is glycerol and 1,3-propanediol in a mass ratio of 5:1; the lipid is hydrogenated lecithin; the emulsifier is polyglycerol-10 oleate; the antioxidant is tocopherol; the preservative is p-hydroxyacetophenone and ethylhexylglycerin; and the pH adjuster is hydroxyethylpiperazine ethanesulfonic acid. A method for preparing a liposome composition encapsulating acne-fighting ingredients includes the following steps: S1. Alcohol phase preparation: Emulsifier, lipid, rice bran oil, antioxidant, polyol, and preservative are mixed, heated and kept warm while stirring until homogeneous to obtain an alcohol phase solution; wherein, the stirring temperature is 55℃, the stirring speed is 600rpm, and the stirring time is 15min. S2. Oil phase preparation: The oil from Melaleuca alternifolia leaves was used as the oil phase; S3. Aqueous phase preparation: Mix the pH adjuster, African cardamom seed extract, white willow bark extract, gentian root extract, kudzu root extract and yeast / zinc fermentation product, and stir evenly to obtain an aqueous phase solution; wherein, the stirring temperature is 25℃, the stirring speed is 600rpm and the time is 15min. S4. Add the oil phase dropwise to the alcohol phase solution and stir at high speed. Then add the aqueous phase solution dropwise and stir at high speed to form a primary emulsion. The high-speed stirring temperature is 55℃, the speed is 1500rpm, and the time is 15min. S5. Homogenize the colostrum using a microfluidic homogenizer to obtain liposomes, then add colloidal sulfur and stir to obtain the liposome composition encapsulating the acne-removing ingredients; wherein the homogenization pressure is 150 MPa and the time is 10 min.

[0028] Example 2 A liposome composition encapsulating acne-fighting ingredients comprises the following components by weight percentage: 1% colloidal sulfur, 1% African cardamom seed extract, 0.2% white willow bark extract, 0.5% gentian root extract, 1% pueraria lobata root extract, 0.1% yeast / zinc fermentation product, 1% Melaleuca alternifolia leaf oil, 0.01% rice bran oil, 5% emulsifier, 5% lipids, 40% polyol, 0.01% antioxidant, 0.1% pH adjuster, and 0.01% preservative, with the balance being... Water; wherein the polyol is glycerol, 1,3-propanediol and 1,2-pentanediol in a mass ratio of 6:2:3; the lipid is hydrogenated lecithin and phosphatidylcholine in a mass ratio of 1:1; the emulsifier is polyglycerol-10 laurate, cetearyl alcohol and PEG-40 hydrogenated castor oil in a mass ratio of 1:0.5:0.3; the antioxidant is tocopherol; the preservative is p-hydroxyacetophenone and ethylhexylglycerin; and the pH adjuster is hydroxyethylpiperazine ethanesulfonic acid. A method for preparing a liposome composition encapsulating acne-fighting ingredients includes the following steps: S1. Alcohol phase preparation: Emulsifier, lipid, rice bran oil, antioxidant, polyol, and preservative are mixed, heated and kept warm while stirring until homogeneous to obtain an alcohol phase solution; wherein, the stirring temperature is 50℃, the stirring speed is 500rpm, and the stirring time is 20min. S2. Oil phase preparation: The oil from Melaleuca alternifolia leaves was used as the oil phase; S3. Aqueous phase preparation: The pH adjuster, African cardamom seed extract, white willow bark extract, gentian root extract, kudzu root extract and yeast / zinc fermentation product were mixed and stirred evenly to obtain an aqueous phase solution; wherein, the stirring temperature was 20℃, the stirring speed was 500rpm and the time was 20min. S4. Add the oil phase dropwise to the alcohol phase solution and stir at high speed. Then add the aqueous phase solution dropwise and stir at high speed to form a primary emulsion. The high-speed stirring temperature is 50℃, the speed is 1000rpm, and the time is 20min. S5. Homogenize the colostrum using a microfluidic homogenizer to obtain liposomes, then add colloidal sulfur and stir to obtain the liposome composition encapsulating the acne-removing ingredients; wherein the homogenization pressure is 50 MPa and the time is 15 min.

[0029] Example 3 A liposome composition encapsulating acne-fighting ingredients comprises the following components by weight percentage: 5% colloidal sulfur, 1% African cardamom seed extract, 0.4% white willow bark extract, 1% gentian root extract, 1% pueraria lobata root extract, 1% yeast / zinc fermentation product, 3% Melaleuca alternifolia leaf oil, 1% rice bran oil, 20% emulsifier, 10% lipids, 60% polyol, 1% antioxidant, 0.5% pH adjuster, and 0.5% preservative, with the balance being water; wherein the polyol is glycerol and 1,2-pentanediol in a mass ratio of 6:1; the lipid is hydrogenated lecithin and dipalmitoylphosphatidylcholine in a mass ratio of 2:1; the emulsifier is polyglycerol-10 oleate; the antioxidant is tocopherol; the preservative is p-hydroxyacetophenone and ethylhexylglycerin; and the pH adjuster is hydroxyethylpiperazine ethanesulfonic acid. A method for preparing a liposome composition encapsulating acne-fighting ingredients includes the following steps: S1. Alcohol phase preparation: Emulsifier, lipid, rice bran oil, antioxidant, polyol and preservative are mixed, heated and kept warm and stirred evenly to obtain alcohol phase solution; wherein, the stirring temperature is 60℃, the stirring speed is 1000rpm and the time is 10min. S2. Oil phase preparation: The oil from Melaleuca alternifolia leaves was used as the oil phase; S3. Aqueous phase preparation: Mix the pH adjuster, African cardamom seed extract, white willow bark extract, gentian root extract, kudzu root extract and yeast / zinc fermentation product, and stir evenly to obtain an aqueous phase solution; wherein, the stirring temperature is 30℃, the stirring speed is 1000rpm and the time is 10min. S4. Add the oil phase dropwise to the alcohol phase solution and stir at high speed. Then add the aqueous phase solution dropwise and stir at high speed to form a primary emulsion. The high-speed stirring temperature is 60℃, the speed is 2000rpm, and the time is 10min. S5. Homogenize the colostrum using a microfluidic homogenizer to obtain liposomes, then add colloidal sulfur and stir to obtain the liposome composition encapsulating the acne-removing ingredients; wherein the homogenization pressure is 250 MPa and the time is 5 min.

[0030] Example 4 The only difference between Example 4 and Example 1 is that the total amount of African cardamom seed extract, white willow bark extract, gentian root extract and kudzu root extract remains unchanged, and the weight ratio of African cardamom seed extract, white willow bark extract, gentian root extract and kudzu root extract is 1:0.6:0.3:1.

[0031] Example 5 The only difference between Example 5 and Example 1 is that the total amount of African cardamom seed extract, white willow bark extract, gentian root extract and kudzu root extract remains unchanged, and the weight ratio of African cardamom seed extract, white willow bark extract, gentian root extract and kudzu root extract is 1:1:0.6:0.3.

[0032] Example 6 The only difference between Example 6 and Example 1 is that the total amount of African cardamom seed extract, white willow bark extract, gentian root extract and kudzu root extract remains unchanged, and the weight ratio of African cardamom seed extract, white willow bark extract, gentian root extract and kudzu root extract is 0.3:1:0.6:1.

[0033] Comparative Example 1 The only difference between Comparative Example 1 and Example 1 is that African cardamom seed extract, white willow bark extract, gentian root extract and kudzu root extract are not added to the composition, and the missing amounts are made up with water.

[0034] Comparative Example 2 The only difference between Comparative Example 2 and Example 1 is that African cardamom seed extract is not added to the composition, and white willow bark extract, gentian root extract and kudzu root extract in a weight ratio of 0.3:0.6:1 are used to make up the missing amount.

[0035] Comparative Example 3 The only difference between Comparative Example 3 and Example 1 is that white willow bark extract is not added to the composition, and African cardamom seed extract, gentian root extract and kudzu root extract in a weight ratio of 1:0.6:1 are used to make up the missing amount.

[0036] Comparative Example 4 The only difference between Comparative Example 4 and Example 1 is that: gentian root extract was not added to the composition, and African cardamom seed extract, white willow bark extract and kudzu root extract in a weight ratio of 1:0.3:1 were used to make up the missing amount.

[0037] Comparative Example 5 The only difference between Comparative Example 5 and Example 1 is that: no Pueraria lobata extract was added to the composition, and African cardamom seed extract, white willow bark extract and gentian root extract in a weight ratio of 1:0.3:0.6 were used to make up the missing amount.

[0038] Comparative Example 6 The only difference between Comparative Example 6 and Example 1 is that no emulsifiers or lipids are added to the composition, water is used to make up for the missing amount, and the preparation method of the composition includes the following steps: S1. Alcohol phase preparation: Rice bran oil, antioxidant, polyol and preservative are mixed, heated and kept warm and stirred evenly to obtain an alcohol phase solution; wherein, the stirring temperature is 55℃, the stirring speed is 600rpm and the time is 15min; S2. Oil phase preparation: The oil from Melaleuca alternifolia leaves was used as the oil phase; S3. Aqueous phase preparation: Mix the pH adjuster, African cardamom seed extract, white willow bark extract, gentian root extract, kudzu root extract and yeast / zinc fermentation product, and stir evenly to obtain an aqueous phase solution; wherein, the stirring temperature is 25℃, the stirring speed is 600rpm and the time is 15min. S4. Add the oil phase dropwise to the alcohol phase solution and stir at high speed. Then add the aqueous phase solution dropwise and stir at high speed to form a primary emulsion. Add colloidal sulfur and stir to obtain the composition. The high-speed stirring temperature is 55℃, the speed is 1500rpm, and the time is 15min.

[0039] Test Example 1: Inhibition Test of the Composition on Propionibacterium acnes The specific testing method is as follows: (1) The compositions of Examples 1-6 and Comparative Examples 1-6 were diluted with deionized water to obtain a test solution with a mass concentration of 2%; (2) Prepare a double-layer plate containing Propionibacterium acnes (P. acnes) (the lower layer is 15 mL of culture medium, and the upper layer is 5 mL of culture medium containing Propionibacterium acnes). The bacterial count of the plate is approximately 10. 5 CFU / plate. Place two Oxford cups vertically on each plate, and add 200 μL of test solution to each Oxford cup. Incubate anaerobically for 24 h at 36 ± 1 °C. Then measure the diameter of the inhibition zone. The larger the diameter of the inhibition zone, the better the antibacterial effect against Propionibacterium acnes. Specific data are shown in Table 1.

[0040] Test Example 2: Inhibition Test of Composition on the Inflammatory Factor TNF-α The specific testing method is as follows: (1) The compositions of Examples 1-6 and Comparative Examples 1-6 were diluted with deionized water to obtain a test solution with a concentration of 2 mg / mL; (2) Dilute the well-cultured Raw264.7 cells to a concentration of 1×10⁻⁶. 5 CFU / mL; then prepare a 12-well cell plate with a concentration of 1×10⁻⁶ CFU / mL. 5 Raw264.7 cells at cfu / mL were pipetted into 12-well plates, 1 mL per well, and incubated in an incubator at 37°C and 5% CO2 for 24 h. (3) Grouping: blank group (no sample and LPS added), LPS group (1 μg / mL LPS added), test group (1 μg / mL LPS added and test solution of each group at 2 mg / mL added); (4) Discard the supernatant of Raw264.7 cell culture medium, add the corresponding solution of each group, and culture at 37℃ for 24h; after the culture is completed, aspirate the supernatant and centrifuge, and detect the concentration of inflammatory factor TNF-α according to the test steps on the ELISA kit. Each group is measured in parallel 3 times and the average value is taken. (5) The TNF-α inhibition rate of the experimental group was calculated using the following formula: TNF-α inhibition rate (%) = [(LPS group inflammatory factor concentration - experimental group inflammatory factor concentration) / (LPS group inflammatory factor concentration - blank group inflammatory factor concentration)] × 100%; the higher the TNF-α inhibition rate, the better the anti-inflammatory effect. Specific data are shown in Table 1.

[0041] Test Example 3: Inhibition Test of Composition on 5α-Reductase 5α-reductase is a membrane protease dependent on reduced coenzyme II (NADPH) and is an important enzyme in the skin that metabolizes male hormones. It irreversibly converts testosterone into dihydrotestosterone (DHT), the most potent androgen, which can induce excessive sebum secretion from the sebaceous glands. Therefore, inhibiting 5α-reductase activity to reduce DHT levels can effectively alleviate excessive sebum secretion, thereby controlling oil production.

[0042] The specific testing method is as follows: (1) Sample tube: Add 1 mL of sample stock solution, 1 mL of enzyme solution, 1 mL of NADPH solution and 1 mL of testosterone solution to a test tube, shake gently, and use a pipette to transfer 200 μL to a 96-well microplate. Perform three replicates for each sample. Place the plate in a microplate reader and measure the absorbance at 340 nm. This is the first measurement value A. 样品0 After incubating the sample at 37°C for 20 minutes, it was placed in an ELISA reader for detection. The absorbance was measured at 340 nm, which is the second measurement value A. 样品20 .

[0043] (2) Enzyme tubes: Add 1 mL of PBS solution, 1 mL of enzyme solution, 1 mL of NADPH solution and 1 mL of testosterone solution to a test tube, shake gently, and use a pipette to transfer 200 μL to a 96-well microplate. Perform three replicates for each sample. Place the plate in a microplate reader and measure the absorbance at 340 nm. This value is the first measurement A. 酶0 After incubating the sample at 37°C for 20 minutes, it was placed in an ELISA reader for detection. The absorbance was measured at 340 nm, which is the second measurement value A. 酶20 Simultaneously perform blank tests, repeat the above experiments 3 times, and take the average.

[0044] (3) The 5α-reductase inhibition rate is calculated using the following formula: 5α-reductase inhibition rate = [1 - (A 样品0 -A 样品20 ) / (A 酶0 -A 酶20 ()×100%. The higher the 5α-reductase content of the composition, the better its oil-controlling effect. See Table 1 for specific data.

[0045] Table 1 Test results for each composition Group / Performance Diameter of the inhibition zone of Propionibacterium acnes / mm TNF-α inhibition rate / % 5α-Reductase Inhibition Rate / % Example 1 32.4 65.9 90.2 Example 2 29.6 61.7 87.6 Example 3 34.0 68.3 94.6 Example 4 27.6 55.4 75.7 Example 5 26.4 52.8 73.4 Example 6 29.7 58.6 80.5 Comparative Example 1 18.4 38.4 54.1 Comparative Example 2 21.6 47.0 61.7 Comparative Example 3 20.6 43.2 64.2 Comparative Example 4 22.5 41.4 59.8 Comparative Example 5 23.5 45.2 57.1 Comparative Example 6 23.8 48.3 66.9 As shown in Table 1, and in conjunction with the data from Examples 1 and 4-6, under the premise of the same amount added, when the weight ratio of African cardamom seed extract, white willow bark extract, gentian root extract and kudzu root extract is 1:(0.2-0.4):(0.5-1):1, the composition has better antibacterial, anti-inflammatory and oil-controlling effects.

[0046] Based on the data from Example 1 and Comparative Examples 1-5, it can be seen that when one of the following is missing: African cardamom seed extract, white willow bark extract, gentian root extract, and kudzu root extract, the antibacterial, anti-inflammatory, and oil-controlling effects of the composition are all lower than those of Example 1. This indicates that African cardamom seed extract, white willow bark extract, gentian root extract, and kudzu root extract can synergistically improve the antibacterial, anti-inflammatory, and oil-controlling effects of the composition.

[0047] Based on the data from Example 1 and Comparative Example 6, it can be seen that when the acne-removing ingredients are encapsulated in liposomes, the composition exhibits better antibacterial, anti-inflammatory, and oil-controlling effects than the composition without encapsulation in liposomes.

[0048] Application Example 1-6 and Comparative Application Example 1-6 The liposome compositions encapsulating acne-removing ingredients of Examples 1-6 and Comparative Examples 1-6 were added to the emulsions at a concentration of 2.5 wt% to obtain the emulsions of Application Examples 1-7 and Comparative Application Examples 1-5. The formulations are shown in Table 2.

[0049] The methods for preparing emulsions in Application Examples 1-6 and Comparative Application Examples 1-6 include the following steps: The method for preparing an emulsion includes the following steps: S1. Mix the moisturizer, thickener and 1 / 2 volume of deionized water, homogenize at 80°C to obtain an emulsion, then add the emulsifier and stir evenly to obtain an emulsion. S2. When the temperature of the emulsion in S1 drops to 60°C, add the preservative. When the system temperature drops to 40°C, add the liposome composition encapsulating the acne-removing ingredients, fragrance, and the remaining deionized water. Stir well and finally add the pH adjuster to obtain the emulsion.

[0050] Table 2. Emulsion formulations of Application Examples 1-6 and Comparative Application Examples 1-6

[0051] Comparative Application Example 7 Compared to the lotion in Application Example 7, which does not contain liposome composition encapsulating acne-fighting ingredients, an equal amount of deionized water was used instead of the composition, and the preparation method was the same as in Application Example 1.

[0052] Test Example 4: Human Efficacy Test of Lotion for Acne Treatment Test standard: T / TDCA004-2021 Test method for acne-removing efficacy of cosmetics.

[0053] Test samples: Emulsions from Application Examples 1-6 and Comparative Application Examples 1-7 above.

[0054] Subjects: Age 18-60 years, with mild acne on the face (such as papules or pustules ≤10) and independent acne marks with a diameter ≥3mm, oily / combination skin, no other skin diseases that may affect the test results, no use of other products with acne-removing effects during the test period, and no allergic reactions to the product being tested. A total of 65 people were randomly divided into 13 groups of 5 people each.

[0055] Testing Method: Subjects used the test sample continuously for 14 days, twice daily. After cleansing, an appropriate amount of the test sample was applied to the acne area and massaged until absorbed. Before using the test product (D0) and after 14 days of use (D14), the percentage of red area on the face (left / middle / right) was measured. The number of acne lesions was assessed by a dermatologist. The improvement rate (%) of red area percentage and the reduction rate (%) of the number of acne lesions after 14 days of use (D14) were calculated as follows: Improvement rate (%) of red area percentage = [(Red area percentage in D14 - Red area percentage in D0) / Red area percentage in D0] × 100%; Reduction rate (%) of the number of acne lesions = [(Number of acne lesions in D14 - Number of acne lesions in D0) / Number of acne lesions in D0] × 100%. The test results are shown in Table 3. The smaller the values ​​of red area percentage and number of acne lesions, the better the acne-removing and repairing effect of the lotion.

[0056] Table 3. Results of human efficacy tests for each group of emulsions. Group / Performance Improvement rate of red area ratio / % Acne lesion reduction rate / % Application Example 1 63.7 92.4 Application Example 2 60.4 88.3 Application Example 3 66.2 95.8 Application Example 4 60.7 84.5 Application Example 5 58.9 82.9 Application Example 6 57.4 87.3 Comparative Application Example 1 44.6 64.1 Comparative Application Example 2 51.3 74.5 Comparative Application Example 3 48.6 69.7 Comparative Application Example 4 46.1 66.2 Comparative Application Example 5 49.5 71.1 Comparative Application Example 6 51.8 76.2 Comparative Application Example 7 5.4 8.9 As shown in Table 3, compared with Comparative Application Example 7, Application Examples 1-6 and Comparative Application Examples 1-6 showed better repair and acne-removing effects of the lotion, indicating that the liposome composition encapsulating acne-removing ingredients is an important parameter affecting the repair and acne-removing effects.

[0057] Based on the data from Application Examples 1 and 4-6, it can be seen that, under the premise of the same amount of composition added, when the weight ratio of African cardamom seed extract, white willow bark extract, gentian root extract and kudzu root extract is 1:(0.2-0.4):(0.5-1):1, the emulsion has better repair and acne-removing effects.

[0058] Based on the data from Application Example 1 and Comparative Application Examples 1-5, it can be seen that when one of the following is missing: African cardamom seed extract, white willow bark extract, gentian root extract, and pueraria lobata extract, the repair and acne-removing effects of the lotion are lower than those in Example 1. This indicates that African cardamom seed extract, white willow bark extract, gentian root extract, and pueraria lobata extract can synergistically improve the repair and acne-removing effects of the lotion.

[0059] Combining the data from Application Example 1 and Comparative Application Example 6, it can be seen that the emulsion containing the composition encapsulated by liposomes has better repair and acne-removing effects than the emulsion without liposome encapsulation. This indicates that due to the encapsulation property of the liposome carrier, the acne-removing ingredients can be released gently, making it gentler on the skin. Moreover, liposomes can improve the dispersibility, stability, and absorption of the acne-removing ingredients in water, thereby improving the repair and acne-removing effects of the emulsion.

[0060] Test Example 5: Human Patch Test of Emulsion Twenty volunteers were recruited, ten men and ten women, aged 20-50 years. A closed patch test method was used. Equal amounts (1g-1.2g) of test samples (emulsions prepared in Application Examples 1-6 and Control Examples 1-6) were placed in a specific patch applicator. The patch was then applied to the volunteers' arms with hypoallergenic adhesive tape. Six sets of test samples were applied to each arm, and gentle pressure was applied to ensure even adhesion to the skin. The patch was left on for 24 hours. After 24 hours, the patch applicator was removed, and skin reactions were observed at 0.5 hours, 24 hours, and 48 hours. The results were recorded. The severity of adverse skin reactions is shown in Table 4 below.

[0061] Table 4. Adverse skin reaction grades Rating levels Skin reaction 0 negative reaction 1 Suspicious reaction, only slight erythema 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and papules may be present. 3 Strong positive reaction (herpes reaction): erythema, infiltration, edema, papules; the reaction may extend beyond the test area. 4 Extremely positive reaction (confluent herpes simplex reaction): marked erythema, severe infiltration, edema, confluent herpes simplex; reaction extends beyond the test area. After testing, the results of the lotion-based human patch tests for Application Examples 1-6 and Comparative Application Examples 2-5 were all negative. However, for Comparative Application Example 1, 8 people showed negative reactions, 1 person had a Grade 1 reaction, and 1 person had a Grade 2 reaction. For Comparative Application Example 6, 4 people showed negative reactions, 3 people had a Grade 1 reaction, and 3 people had a Grade 2 reaction. This demonstrates that liposome encapsulation improves the gentleness of the acne-treating composition, and the extracts of African cardamom seed, white willow bark, gentian root, and kudzu root further reduce the irritation of the composition.

[0062] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A liposome composition encapsulating acne-fighting ingredients, characterized in that, It comprises the following components by weight percentage: 1-5% colloidal sulfur, 0.1-1% African cardamom seed extract, 0.1-1% white willow bark extract, 0.1-1% gentian root extract, 0.1-1% kudzu root extract, 0.1-1% yeast / zinc fermentation product, 1-3% Melaleuca alternifolia leaf oil, 0.01-1% rice bran oil, 1-20% emulsifier, 1-10% lipids, 40-60% polyol, 0.01-1% antioxidant, 0.1-0.5% pH adjuster, and 0.01-0.5% preservative, with the balance being water.

2. The liposome composition encapsulating acne-reducing ingredients as described in claim 1, characterized in that, The weight ratio of the African cardamom seed extract, white willow bark extract, gentian root extract, and kudzu root extract is 1:(0.2-0.4):(0.5-1):

1.

3. The liposome composition encapsulating acne-reducing ingredients as described in claim 1, characterized in that, The polyol is at least one selected from glycerol, 1,3-propanediol, 1,2-pentanediol, and hexanediol; the lipid is at least one selected from hydrogenated lecithin, soybean lecithin, phosphatidylcholine, distearyl phosphatidylcholine, and dipalmitoyl phosphatidylcholine; and the emulsifier is at least one selected from polyglycerol-10 oleate, polyglycerol-10 laurate, cetearyl alcohol, polysorbate-20, and PEG-40 hydrogenated castor oil.

4. The liposome composition encapsulating acne-reducing ingredients as described in claim 1, characterized in that, The antioxidant is tocopherol; the preservative is p-hydroxyacetophenone and ethylhexylglycerin; and the pH adjuster is hydroxyethylpiperazine ethanesulfonic acid.

5. A method for preparing the liposome composition encapsulating acne-removing ingredients according to any one of claims 1-4, characterized in that, Includes the following steps: S1. Alcohol phase preparation: Emulsifier, lipid, rice bran oil, antioxidant, polyol and preservative are mixed, heated and kept warm and stirred evenly to obtain alcohol phase solution; S2. Oil phase preparation: The oil from Melaleuca alternifolia leaves was used as the oil phase; S3. Aqueous phase preparation: Mix the pH adjuster, African cardamom seed extract, white willow bark extract, gentian root extract, kudzu root extract and yeast / zinc fermentation product, stir well to obtain an aqueous phase solution. S4. Add the oil phase dropwise to the alcohol phase solution, stir at high speed, then add the aqueous phase solution dropwise, and stir at high speed to form the primary emulsion. S5. Homogenize the colostrum using a microfluidic homogenizer to obtain liposomes, then add colloidal sulfur and stir to obtain the liposome composition encapsulating the acne-removing ingredients.

6. The method for preparing the liposome composition encapsulating acne-removing ingredients as described in claim 5, characterized in that, In step S1, the stirring temperature is 50-60℃, the stirring speed is 500-1000rpm, and the stirring time is 10-20min.

7. The method for preparing the liposome composition encapsulating acne-removing ingredients as described in claim 5, characterized in that, In step S3, the stirring temperature is 20-30℃, the stirring speed is 500-1000rpm, and the stirring time is 10-20min.

8. The method for preparing the liposome composition encapsulating acne-removing ingredients as described in claim 5, characterized in that, In step S4, the high-speed stirring temperature is 50-60℃, the rotation speed is 1000-2000rpm, and the time is 10-20min.

9. The method for preparing the liposome composition encapsulating acne-removing ingredients as described in claim 5, characterized in that, In step S5, the homogenization pressure is 50-250 MPa and the time is 5-15 min.

10. The use of the liposome composition encapsulating acne-removing ingredients according to any one of claims 1-4 in the preparation of acne-removing products.

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