A green extraction process for high-purity, high-activity quinoa polysaccharides

Abstract

This invention belongs to the field of plant effective component separation and extraction technology, specifically involving a green extraction process for high-purity and high-activity quinoa polysaccharides. The extraction process includes the following steps: (1) Ultrasonic-microwave pretreatment: Take quinoa bran or endosperm residue, wash, dry, and crush to obtain quinoa matrix powder; break the cell wall of the quinoa matrix powder to obtain a pretreated extract; centrifuge the pretreated extract and collect the supernatant; (2) Enzymatic hydrolysis and directional decontamination: Add a compound enzyme to the supernatant for temperature-controlled enzymatic hydrolysis, raise the temperature, cool after enzymatic hydrolysis, centrifuge, and collect the decontaminated polysaccharide solution; (3) Macroporous resin-membrane coupled purification: Adsorb the decontaminated polysaccharide solution onto a macroporous resin, elute and enrich, and ultrafilter to obtain a purified polysaccharide solution; (4) Concentration and drying: Concentrate the purified polysaccharide solution under vacuum and freeze-dry to obtain the final product. The extraction process provided by this invention has high efficiency, excellent purification effect, and low energy consumption.

Description

Technical Field

[0001] This invention belongs to the field of separation and extraction technology of plant active ingredients, specifically involving a green extraction process for high-purity and high-activity quinoa polysaccharides. Background Technology

[0002] Quinoa, a grain rich in nutrients, contains a variety of bioactive components, among which polysaccharides are one of the important active substances. Studies have shown that quinoa polysaccharides have various physiological activities such as antioxidation, immune regulation, and hypoglycemia, and have broad application prospects in the fields of food, medicine, and health products.

[0003] Currently, polysaccharide extraction methods mainly include hot water extraction, ultrasound-assisted extraction, microwave-assisted extraction, and enzymatic hydrolysis. Existing technologies for quinoa polysaccharide extraction often suffer from the following drawbacks: low extraction efficiency, long extraction time leading to glycosidic bond breakage and activity loss; difficult purification, with high levels of protein and starch impurities in the crude extract; traditional Sevag deproteinization requires repeated operations 3-5 times, which is time-consuming and results in significant polysaccharide loss; and insufficient environmental friendliness: some processes use strong acid / base assisted extraction, which, while increasing the extraction rate, damages the polysaccharide structure and generates chemical wastewater, leading to high environmental treatment costs.

[0004] Relevant patent documents retrieved:

[0005] For example, Chinese patent CN112759664A, published on May 13, 2022, discloses a method for preparing quinoa small molecule heteropolysaccharides, including the following steps: S1. Selecting and crushing quinoa raw materials, extracting by ethanol reflux, and preparing distillate; S2. Treating twice with α-amylase to obtain a heat-insulating solution; S3. Preparing a supernatant; S4. Washing with twice the volume of anhydrous ethanol to prepare refined quinoa non-starch polysaccharides; S5. Using "ultrasound-hydrogen peroxide-vitamin C-ultrafiltration"... Online cyclic degradation yields a polysaccharide degradation solution; S6. Adsorption by anion exchange resin, followed by desorption and ultrafiltration through an ultrafiltration membrane, yielding 150g of quinoa small molecule heteropolysaccharide; The beneficial effects of this invention are: by first pulverizing quinoa, then soaking it in PBS buffer, then treating it twice with α-amylase, followed by pretreatment, and then treating it with anhydrous ethanol, followed by online cyclic degradation, and finally treatment with anion exchange resin and ultrafiltration membrane, quinoa small molecule heteropolysaccharide with high purity and relatively uniform molecular weight is obtained.

[0006] Relevant non-patent literature retrieved:

[0007] The journal or book title is *Food Research and Development*, and the article title is "Enzymatic Hydrolysis and Ultrasonic Co-extraction of Quinoa Polysaccharides and Evaluation of In Vitro Activity," volume number 2019.08010. This study used a combined enzymatic hydrolysis and ultrasonic method to extract polysaccharides from quinoa. The optimal coenzyme was determined to be cellulase, with an optimal addition amount of 3%. Based on single-factor experiments, response surface methodology was conducted. The results showed that the optimal extraction process for quinoa polysaccharides was: ultrasonic temperature 65℃, ultrasonic time 18 min, and a material-to-liquid ratio of 1:33 (g / mL). Under these conditions, the extraction rate of quinoa polysaccharides was 68.08%, close to the theoretical value of 70.78%. After deproteinization, the crude polysaccharides extracted under the above optimal conditions were subjected to in vitro activity studies. Antioxidant tests showed that quinoa polysaccharides had a significant scavenging effect on hydroxyl radicals, but no significant scavenging effect on DPPH and ABTS+ radicals. Furthermore, quinoa polysaccharides had a certain inhibitory effect on α-amylase activity, with an inhibition rate of up to 27.29%. This indicates that quinoa polysaccharides have a certain effect in lowering blood sugar. However, its extraction rate still needs to be improved.

[0008] Therefore, there is an urgent need to develop a low-damage, high-efficiency, and low-pollution quinoa polysaccharide extraction process to solve the problem that existing technologies cannot simultaneously achieve "efficiency-purity-activity". Summary of the Invention

[0009] The purpose of this invention is to provide a green extraction process for high-purity and high-activity quinoa polysaccharides, and related technologies, to solve technical problems such as low extraction efficiency and low activity, or combinations thereof.

[0010] Terminology Explanation:

[0011] Unless otherwise defined, all technical terms in this document have the same meanings as commonly understood by one of ordinary skill in the art to which the subject matter of the claims pertains. Unless otherwise stated, all patents, patent inventions, and publications cited in this document are incorporated herein by reference in their entirety. If multiple definitions exist for terms in this document, the definitions in this chapter shall prevail.

[0012] It should be understood that the above brief description and the following detailed description are exemplary and for illustrative purposes only, and do not limit the subject matter of the invention in any way. In this invention, the singular is used in conjunction with the plural unless otherwise specifically stated. It should also be noted that, unless otherwise stated, the use of “or” or “or” means “and / or”. Furthermore, the use of the term “comprising” and other forms such as “including,” “containing,” and “contains” are not limiting.

[0013] The definition of the standard chemical term can be found in the reference "Natural Product Extraction Technology (Second Edition)," China Light Industry Press, August 2020.

[0014] Unless specifically defined herein, the use of all commercially available products herein employs standard techniques. For example, it may be carried out using the manufacturer's instructions for use with the kit, or in accordance with methods known in the art or the description of this invention. The techniques and methods described herein can generally be implemented according to conventional methods well known in the art, based on the descriptions in the various summary and more specific documents cited and discussed in this specification.

[0015] In a first aspect, the present invention provides a green extraction process for high-purity, high-activity quinoa polysaccharides, comprising the following steps:

[0016] (1) Ultrasonic-microwave pretreatment: Take quinoa bran or endosperm residue, wash, dry, and crush to obtain quinoa matrix powder; break the cell wall of the quinoa matrix powder to obtain pretreatment extract; centrifuge the pretreatment extract and collect the supernatant;

[0017] (2) Enzymatic hydrolysis for targeted decontamination: Add a complex enzyme to the supernatant for temperature-controlled enzymatic hydrolysis, raise the temperature, cool after enzymatic hydrolysis, centrifuge, and collect the decontaminated polysaccharide solution;

[0018] (3) Purification by macroporous resin-membrane coupling: The polysaccharide solution after impurity removal is adsorbed by macroporous resin, eluted and enriched, and then ultrafiltered and fractionated to obtain purified polysaccharide solution;

[0019] (4) Concentration and drying: The purified polysaccharide solution is concentrated under vacuum and freeze-dried to obtain quinoa polysaccharide powder.

[0020] Preferably, the drying temperature in step (1) is 45-50℃, and the moisture content is 7-9%. The mesh size of the pulverized material is 100-120 mesh. Other specific values ​​within the above range can be selected to achieve the technical effect of the present invention. For example, the mesh size includes but is not limited to 100 mesh, 105 mesh, 110 mesh, 115 mesh, and 120 mesh.

[0021] More preferably, the drying temperature in step (1) is 48°C, the drying is carried out until the moisture content is 8%, and the pulverization mesh size is 110 mesh.

[0022] Preferably, in the cell wall breaking process described in step (1), the quinoa matrix powder and water are placed in an ultrasonic-microwave synergistic extraction tank with a solid-liquid ratio of 1:20-25. The ultrasonic level is set to 300-350W, the microwave level to 400-450W, and the temperature to 50-55℃. The treatment time is 20-30 minutes to destroy the cell wall structure and allow the polysaccharides to be initially dissolved. Other specific values ​​within the above range can be selected to achieve the technical effect of the present invention. For example, the treatment time includes, but is not limited to, 20 minutes, 21 minutes, 22 minutes, 23 minutes, 24 minutes, 25 minutes, 26 minutes, 27 minutes, 28 minutes, 29 minutes, and 30 minutes.

[0023] More preferably, the cell wall breaking process described in step (1) is as follows: according to the solid-liquid ratio of quinoa matrix powder and water of 1g:22mL, the two are placed in an ultrasonic-microwave synergistic extraction tank, and the ultrasonic is set to 320W, the microwave to 420W, and the temperature to 52℃, and the treatment is carried out for 25min.

[0024] Preferably, the centrifugation in step (1) is centrifugation at 3200-3600 r / min for 10-15 min. Other specific values ​​within the above range can be selected, and all can achieve the technical effect of the present invention. For example, the centrifugation time includes, but is not limited to, 10 min, 11 min, 12 min, 13 min, 14 min, and 15 min.

[0025] More preferably, the centrifugation in step (1) is centrifugation at 3500 r / min for 15 min.

[0026] Preferably, the complex enzyme described in step (2) is composed of alkaline protease (enzyme activity 20000U / g) and saccharifying enzyme (enzyme activity 100000U / g), with a mass ratio of 1-3:1. Other specific values ​​within the above range can be selected, and all can achieve the technical effect of the present invention.

[0027] More preferably, the complex enzyme described in step (2) consists of alkaline protease and saccharifying enzyme in a mass ratio of 2:1.

[0028] Preferably, the amount of compound enzyme added in step (2) is 0.8%-1.2% of the mass of quinoa matrix powder. Other specific values ​​within the above range can be selected, and all can achieve the technical effect of the present invention.

[0029] More preferably, the amount of the compound enzyme added in step (2) is 1.0% of the mass of quinoa matrix powder.

[0030] Preferably, the temperature-controlled enzymatic hydrolysis in step (2) is carried out at a temperature of 55-60℃ and a pH of 7.5-8.0 for 1.5-2 hours, with a stirring rate of 130-160 r / min during the period, so that the protein is hydrolyzed into small molecule peptides (which are easy to separate later) and the starch is hydrolyzed into glucose. Other specific values ​​within the above range can be selected, and all can achieve the technical effect of the present invention. For example, the holding time includes but is not limited to 1.5h, 1.6h, 1.7h, 1.8h, 1.9h, and 2h.

[0031] More preferably, the temperature-controlled enzymatic hydrolysis in step (2) is carried out at a temperature of 58°C and a pH of 7.8 for 1.8 hours, during which the stirring rate is 150 r / min.

[0032] Preferably, the temperature for heating in step (2) is 75-80℃, and the temperature is maintained for 5-15 minutes; the centrifugation is carried out at 3500-4500 r / min for 15-25 minutes. Other specific point values ​​within the above range can be selected, and all can achieve the technical effect of the present invention. For example, the temperature includes but is not limited to 75℃, 76℃, 77℃, 78℃, 79℃, and 80℃.

[0033] More preferably, the temperature for heating in step (2) is 78°C and the temperature is maintained for 10 min; the centrifugation is carried out at 4000 r / min for 20 min.

[0034] Preferably, the macroporous resin column used for adsorption in step (3) is of type D101, with a column diameter:column height ratio of 1:8 and a flow rate of 2-3 BV / h. Other specific point values ​​within the above range can be selected, and all can achieve the technical effect of the present invention. For example, the flow rate includes, but is not limited to, 2 BV / h, 2.5 BV / h, and 3 BV / h.

[0035] More preferably, the macroporous resin column used for adsorption in step (3) is of type D101, with a column diameter: column height ratio of 1:8 and a flow rate of 2.5 BV / h.

[0036] Preferably, the elution enrichment in step (3) uses a 0.3-0.5 mol / L ethanol solution to elute the resin at a flow rate of 1.5-2 BV / h, and collects the eluent (polysaccharide content ≥80%). Other specific values ​​within the above range can be selected, and all can achieve the technical effect of the present invention. For example, the flow rate includes, but is not limited to, 1.5 BV / h, 1.6 BV / h, 1.7 BV / h, 1.8 BV / h, 1.9 BV / h, and 2 BV / h.

[0037] More preferably, the elution enrichment in step (3) is carried out by eluting the resin with a 0.4 mol / L ethanol solution and an elution flow rate of 1.8 BV / h.

[0038] Preferably, the ultrafiltration fractionation in step (3) involves passing the eluent through a 10000 Da hollow fiber membrane for ultrafiltration, retaining active polysaccharide fragments with a molecular weight of 30000-80000 Da, and reusing the permeate (containing small molecule impurities) to obtain a purified polysaccharide solution (purity ≥90%). Other specific point values ​​within the above range can be selected, and all can achieve the technical effects of the present invention.

[0039] Preferably, the vacuum concentration in step (4) involves placing the purified polysaccharide solution in a vacuum concentration tank and concentrating it to a polysaccharide mass concentration of 40%-45% under conditions of a temperature of 60-65℃ and a vacuum degree of -0.08 to -0.09 MPa. Other specific point values ​​within the above range can be selected, and all can achieve the technical effect of the present invention. For example, the temperature includes but is not limited to 60℃, 61℃, 62℃, 63℃, 64℃, and 65℃.

[0040] More preferably, the vacuum concentration in step (4) involves placing the purified polysaccharide solution in a vacuum concentration tank and concentrating it to a polysaccharide mass concentration of 42% under conditions of a temperature of 62°C and a vacuum degree of -0.085MPa.

[0041] Preferably, the freeze drying in step (4) involves placing the concentrated liquid in a freeze dryer, setting the pre-freezing temperature to -40 to -45°C (holding for 2 hours), the sublimation temperature to 30-35°C, the vacuum degree to 10-15 Pa, and drying for 8-10 hours to obtain white quinoa polysaccharide powder (moisture content <2%). Other specific values ​​within the above range can be selected, and all can achieve the technical effect of the present invention. For example, the vacuum degree includes, but is not limited to, 10 Pa, 11 Pa, 12 Pa, 13 Pa, 14 Pa, and 15 Pa.

[0042] More preferably, the freeze drying in step (4) involves placing the concentrate in a freeze dryer, setting the pre-freezing temperature to -42°C (holding for 2 hours), the sublimation temperature to 32°C, the vacuum degree to 12Pa, and drying for 9 hours.

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] The extraction process provided by this invention uses a combination of enzymes to increase extraction efficiency and obtain quinoa polysaccharides with high activity, exhibiting good free radical scavenging rate and α-glucosidase inhibition rate. Detailed Implementation

[0045] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection claimed by the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and such changes should also fall within the scope of protection claimed by the present invention.

[0046] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all instruments, devices, equipment, reagents, products, etc., used in the embodiments of the present invention are obtained through conventional commercial means.

[0047] The alkaline protease (enzyme activity 20000 U / g) used in this invention was purchased from Novozymes (China) Biotechnology Co., Ltd.; the glucoamylase (enzyme activity 100000 U / g) was purchased from Novozymes (China) Biotechnology Co., Ltd.

[0048] Example 1: A green extraction process for high-purity, high-activity quinoa polysaccharides

[0049] The specific steps are as follows:

[0050] (1) Ultrasonic-microwave pretreatment: Take 300g of quinoa bran, dry it at 48℃ to a moisture content of 8%, and grind it to 110 mesh to obtain quinoa matrix powder; put the quinoa matrix powder and deionized water in an ultrasonic-microwave synergistic extraction tank at a ratio of 1g:22mL, set the ultrasonic to 320W, the microwave to 420W, and the temperature to 52℃, and treat for 25min; centrifuge at 3500r / min for 15min and collect the supernatant;

[0051] (2) Enzymatic hydrolysis for targeted decontamination: Add 1.0% of the mass of quinoa matrix powder of compound enzyme (alkaline protease: saccharifying enzyme = 2:1), incubate at 58℃ and pH 7.8 for 1.8h, inactivate at 78℃ for 10min, centrifuge at 4000r / min for 20min to obtain decontaminated polysaccharide solution. Collect the decontaminated polysaccharide solution.

[0052] (3) Purification by macroporous resin-membrane coupling: The purified polysaccharide solution was passed through a D101 resin column (flow rate 2.5 BV / h) and eluted with 0.4 mol / L ethanol (flow rate 1.8 BV / h). The eluent was collected and purified polysaccharide solution (purity 91.5%) was obtained by ultrafiltration at 100000 Da.

[0053] (4) Concentration and drying: The purified polysaccharide solution was concentrated to a mass concentration of 42% at 62℃ and -0.085MPa vacuum, pre-frozen at -42℃ for 2h, and freeze-dried at 32℃ and 12Pa for 9h to obtain 76.5g of quinoa polysaccharide powder.

[0054] Example 2

[0055] The difference from Example 1 is that the amount of compound enzyme added is 0.8% of the mass of quinoa matrix powder, and the rest is the same as in Example 1, resulting in 72.8g of quinoa polysaccharide powder.

[0056] Example 3

[0057] The difference from Example 1 is that the amount of compound enzyme added is 1.2% of the mass of quinoa matrix powder, and the rest is the same as in Example 1, resulting in 74.7g of quinoa polysaccharide powder.

[0058] Example 4

[0059] The difference from Example 1 is that the mass ratio of alkaline protease and saccharifying enzyme in the complex enzyme is 1:1, while the rest is the same as in Example 1, yielding 70.3g of quinoa polysaccharide powder.

[0060] Example 5

[0061] The difference from Example 1 is that the mass ratio of alkaline protease to saccharifying enzyme in the complex enzyme is 3:1, while the rest is the same as in Example 1, yielding 72.1g of quinoa polysaccharide powder.

[0062] Comparative Example 1

[0063] The difference from Example 1 is that the amount of compound enzyme added is 1.5% of the mass of quinoa matrix powder, and the rest is the same as in Example 1, resulting in 54.2g of quinoa polysaccharide powder.

[0064] Comparative Example 2

[0065] The difference from Example 1 is that the mass ratio of alkaline protease to saccharifying enzyme in the complex enzyme is 1:2, while the rest is the same as in Example 1, yielding 56.6g of quinoa polysaccharide powder.

[0066] Comparative Example 3

[0067] The difference from Example 1 is that the saccharifying enzyme was replaced with α-amylase, and the rest is the same as in Example 1, yielding 51.8g of quinoa polysaccharide powder.

[0068] Comparative Example 4

[0069] The difference from Example 1 is that the solid-liquid ratio of quinoa matrix powder to water is 1g:35mL, while the rest is the same as in Example 1, yielding 53.3g of quinoa polysaccharide powder.

[0070] Comparative Example 5

[0071] The difference from Example 1 is that the temperature-controlled enzymatic hydrolysis in step (2) is carried out at 50°C and pH 8.5 for 1.8 hours. The rest is the same as in Example 1, and 57.2g of quinoa polysaccharide powder is obtained.

[0072] Effect Experiment

[0073] 1. DPPH free radical scavenging rate

[0074] The DPPH and ABTS free radical scavenging rates were detected using a detection kit. First, sample solutions were prepared, including low (1 mg / mL), medium (4 mg / mL), and high (8 mg / mL) dose groups for Example 1; medium dose groups for Examples 2-5 and Comparative Examples 1-5; and a positive control group consisting of low (1 mg / mL), medium (4 mg / mL), and high (8 mg / mL) vitamin C groups. Three replicates were set up. Specific operating steps are as follows:

[0075] Table 1. DPPH Reagent Kit Sample Addition and Detection Procedure

[0076]

[0077] DPPH free radical scavenging rate (%) = [(1-(A)] 测定 -A 样品 )÷A 空白 [)×100%], where A 测定 To measure the absorbance of the tube, A 样品 A represents the absorbance of the sample tube. 空白 The absorbance of the blank tube is given.

[0078] The results are shown in Table 1.

[0079] Table 1

[0080]

[0081] 2. α-glucosidase inhibition rate

[0082] All samples and reagents were prepared using PBS (0.1 mol / L, pH 6.9). 50 μL of quinoa polysaccharides prepared at different concentrations (2, 4, 6, 8, and 10 mg / mL) from each example and comparative example were mixed with 50 μL of α-glucosidase solution (0.2 U / mL) and incubated at room temperature for 6 min. Then, 50 μL of pNPG was added, and the reaction was carried out at 37°C for 10 min. Finally, 50 μL of 0.2 mol / L sodium carbonate solution was added to terminate the reaction. The absorbance was measured at 405 nm. PBS solution was used as a blank group instead of the sample, and PBS solution was used as a control group instead of the α-glucosidase solution.

[0083] α-glucosidase inhibition rate (%) = (1- ) × 100%

[0084] In the formula: A1 is the absorbance of the sample to be tested; A2 is the absorbance of the control group; A0 is the absorbance of the blank group.

[0085] The results are shown in Table 2.

[0086] Table 2

[0087]

[0088] As can be seen from the data in Tables 1 and 2, the quinoa polysaccharide obtained by the extraction process provided in the embodiments of the present invention has a better free radical scavenging rate and a better α-glucosidase inhibition rate.

[0089] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A green extraction process for high-purity, high-activity quinoa polysaccharides, characterized in that, Includes the following steps: (1) Ultrasonic-microwave pretreatment: Take quinoa bran or endosperm residue, wash, dry, and crush to obtain quinoa matrix powder; break the cell wall of the quinoa matrix powder to obtain pretreated extract; Centrifuge the pretreated extract and collect the supernatant; the cell wall breaking process is as follows: place the quinoa matrix powder and water in an ultrasonic-microwave synergistic extraction tank according to the solid-liquid ratio of 1g:20-25mL. (2) Enzymatic hydrolysis for targeted decontamination: Add a complex enzyme to the supernatant for temperature-controlled enzymatic hydrolysis, raise the temperature, cool after enzymatic hydrolysis, centrifuge, and collect the decontaminated polysaccharide solution; (3) Purification by macroporous resin-membrane coupling: The polysaccharide solution after impurity removal is adsorbed by macroporous resin, eluted and enriched, and then ultrafiltered and fractionated to obtain purified polysaccharide solution; (4) Concentration and drying: The purified polysaccharide solution is concentrated under vacuum and freeze-dried to obtain quinoa polysaccharide powder; The complex enzyme described in step (2) consists of alkaline protease and saccharifying enzyme in a mass ratio of 1-3:1, and the amount of complex enzyme added is 0.8%-1.2% of the mass of quinoa matrix powder.

2. The extraction process according to claim 1, characterized in that, The drying temperature in step (1) is 45-50℃, and the moisture content is 7-9%. The mesh size of the pulverized material is 100-120 mesh.

3. The extraction process according to claim 1, characterized in that, In step (1), the cell wall breaking process is performed by setting the ultrasonic power to 300-350W, the microwave power to 400-450W, the temperature to 50-55℃, and the treatment time to 20-30 minutes.

4. The extraction process according to claim 1, characterized in that, The temperature-controlled enzymatic hydrolysis described in step (2) is carried out at a temperature of 55-60℃ and a pH of 7.5-8.0 for 1.5-2 hours, during which the stirring rate is 130-160 r / min.

5. The extraction process according to claim 1, characterized in that, The macroporous resin column used for adsorption in step (3) is of type D101, with a column diameter: column height ratio of 1:8 and a flow rate of 2-3 BV / h.

6. The extraction process according to claim 1, characterized in that, The elution enrichment in step (3) is carried out by eluting the resin with a 0.3-0.5 mol / L ethanol solution and an elution flow rate of 1.5-2 BV / h.

7. The extraction process according to claim 1, characterized in that, The vacuum concentration in step (4) involves placing the purified polysaccharide solution in a vacuum concentration tank and concentrating it to a polysaccharide mass concentration of 40%-45% under conditions of a temperature of 60-65℃ and a vacuum degree of -0.08 to -0.09MPa.

8. The extraction process according to claim 1, characterized in that, The freeze drying in step (4) involves placing the concentrate in a freeze dryer, setting the pre-freezing temperature to -40 to -45°C, holding it at that temperature for 2 hours, setting the sublimation temperature to 30-35°C, the vacuum degree to 10-15Pa, and drying for 8-10 hours.

9. Quinoa polysaccharide obtained by the extraction process according to any one of claims 1-8.

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