Composite microbial flora for pollutant degradation and feeding device thereof

Abstract

The invention relates to the technical field of environmental microorganisms, in particular to a compound microbial flora for pollutant degradation and a feeding device of the compound microbial flora, and the compound microbial flora comprises functional microorganisms, auxiliary microorganisms and a microbial carrier according to the mass ratio of 4: 3: 3. Through the synergistic effect of the functional microorganisms, the auxiliary microorganisms and the microbial carrier, efficient targeted degradation of pollutants is achieved, meanwhile, seaweed growth and rhizosphere micro-ecology stability are promoted by means of the auxiliary microorganisms, self-adaptive putting of the compound microbial flora is achieved through matched putting equipment, and the comprehensive utilization rate of the compound microbial flora is improved. The comprehensive efficiency and the ecological compatibility of pollution remediation of the seaweed bed are improved.

Description

Technical Field

[0001] This invention relates to the field of environmental microbiology, specifically to a composite microbial community for pollutant degradation and its delivery device. Background Technology

[0002] Through long-term natural selection, seagrass bed microorganisms have developed metabolic mechanisms adapted to high-salt, fluctuating-temperature, and oxygen-deficient sediment environments. Functional microorganisms that coexist with seagrass naturally exist in the rhizosphere microecology and can achieve synergistic degradation of pollutants through metabolic interactions, possessing potential for functional development.

[0003] In existing technologies, seagrass bed remediation microbial agents are used to degrade pollution in seagrass beds. These agents are produced by fermenting Bacillus SCSIO 43505 and Asteris SCSIO 43504, isolated and purified from the rhizosphere of seagrass, into bacterial solutions. These solutions are then further concentrated, mixed with suitable carriers such as wheat bran and talc, and dried. After application, the solutions precipitate around the seagrass rhizosphere, facilitating bacterial colonization at the seagrass roots, promoting nitrogen fixation and the release of available phosphorus, thereby promoting seagrass rooting, germination, and growth.

[0004] However, in the actual application of the aforementioned seagrass bed remediation microbial agents for pollutant degradation in seagrass beds, the agents primarily promote seagrass growth, and their ability to directly degrade specific pollutants such as petroleum hydrocarbons and heavy metals is limited, making it difficult to specifically address the diverse pollutant problems faced by seagrass beds. Therefore, it is necessary to propose a composite microbial community and its delivery device for pollutant degradation to solve the above problems. Summary of the Invention

[0005] To address the aforementioned issues, this invention provides a composite microbial community for pollutant degradation and its delivery device. This community achieves highly efficient and targeted degradation of pollutants through the synergistic effect of functional microorganisms, auxiliary microorganisms, and microbial carriers. Simultaneously, it promotes seagrass growth and rhizosphere microecological stability with the help of auxiliary microorganisms, and achieves adaptive delivery of the composite microbial community through a matching delivery device, thereby improving the overall efficiency and ecological compatibility of seagrass bed pollution remediation.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: a composite microbial community for pollutant degradation, comprising functional microorganisms, auxiliary microorganisms and microbial carriers, in a mass ratio of 4:3:3.

[0007] The technical principles of the above solution are as follows:

[0008] Functional microorganisms account for the largest proportion, ensuring the core's ability to degrade various pollutants; auxiliary microorganisms amplify the functional efficiency of the microbial community through ecological interactions, avoiding the metabolic bottleneck of a single microbial community; and microbial carriers provide the microenvironmental support for the colonization of the former two.

[0009] The above solution has the following beneficial effects:

[0010] 1. Through the targeted compatibility of functional microorganisms, this solution achieves the synergistic degradation of various pollutants such as petroleum hydrocarbons, pesticide residues, and heavy metals, breaking through the limitation that existing bacterial agents only focus on promoting seagrass growth and have a single pollutant degradation ability, and can specifically solve the problem of complex pollution in seagrass beds.

[0011] 2. This solution takes the native microorganisms in seagrass beds as the core and is paired with auxiliary microorganisms, which not only ensures the colonization activity of the bacterial community in special environments such as high salinity and hypoxia, but also strengthens the symbiotic relationship between seagrass and microorganisms through growth promotion, realizing the integration of pollution degradation and ecological restoration, and enhancing the stability and persistence of the restoration effect.

[0012] 3. The supporting dosing equipment of this solution can adaptively adjust the dosing of the composite microbial community according to the pollution degree, improve the colonization efficiency of the composite microbial community in the rhizosphere area of seagrass roots, reduce ineffective losses, and is more efficient than traditional manual spreading.

[0013] Furthermore, the functional microorganisms include: Rhodobacter, Desulfobacterium, Sphingomonas, and Nitrospira, and their mass ratio is 3:2:3:2.

[0014] Beneficial effects: At this ratio, Rhodobacter and Sphingomonas, as the leading degrading bacteria, can efficiently decompose petroleum hydrocarbons and pesticide residues, Desulfobacterium specifically reduces the toxicity of sulfides and the activity of heavy metals, and Nitrospira balances the nitrogen cycle, ensuring the synergistic removal efficiency of composite pollutants.

[0015] Furthermore, the auxiliary microorganisms include: Burkholderia, Bacteroidetes, Methylotrophs, and Chloroflexi, and their mass ratio is 3:2:3:2.

[0016] Beneficial effects: Burkholderia and Methylotrophs respectively strengthen the functional chain through growth promotion and degradation product transformation, Bacteroidetes constructs a biofilm to enhance the attachment of the bacterial community, and Chloroflexi enhances the environmental adaptability. This ratio not only ensures the ecological synergistic effect but also avoids the over-competition of auxiliary bacteria from inhibiting the activity of functional microorganisms.

[0017] Furthermore, the microbial carrier includes: zeolite, sodium alginate, trehalose, and xanthan gum, and their mass ratio is 4:3:2:1.

[0018] Beneficial effects: Zeolite provides highly adsorptive colonization sites, sodium alginate forms a protective gel microenvironment, trehalose maintains the activity of the bacterial community during the dormant period, and xanthan gum enhances adhesion, which can improve the survival rate and long-term effectiveness of the bacterial community in the sediments of seagrass beds.

[0019] Furthermore, a composite microbial community dispensing device for pollutant degradation includes a dispensing component for dispensing the composite microbial community; the dispensing component includes a float, a filter steel mesh, an inlet tank, and an outlet tank fixedly connected from top to bottom; the inlet tank and the outlet tank are connected; a channel is opened on the bottom wall of the inlet tank, and the channel is connected to the outlet tank; a microbial community storage tank is fixedly connected to the float; a suction component for pumping seawater into the inlet tank is provided in the outlet tank; and a dispersing component for dispensing the composite microbial community in the microbial community storage tank onto the seawater pumped into the inlet tank is provided in the inlet tank.

[0020] Beneficial effects: The floating board ensures the equipment floats in the seagrass bed waters, and the filter steel mesh can intercept seawater impurities to prevent clogging; the interconnected design of the inlet and outlet tanks enables seawater circulation, and together with the suction and delivery components, it can evenly mix the compound microbial community with seawater and release it in a targeted manner, improving the contact efficiency between the compound microbial community and the seagrass root zone, and reducing the problem of easy loss of compound microbial community when traditionally spread.

[0021] Furthermore, the suction assembly includes a controller and a first support rod fixedly connected to the inner wall of the drainage tank. A waterproof tank is fixedly connected to the first support rod, and a dual-head drive is fixedly connected to the inner wall of the waterproof tank. The controller is used to control the rotation of the output shaft of the dual-head drive. One end of the output shaft of the dual-head drive penetrates through the adjacent side wall of the waterproof tank and is coaxially fixedly connected to a turbine fan blade. The other end of the output shaft of the dual-head drive penetrates through the adjacent side wall of the waterproof tank, extends into the channel, and is coaxially fixedly connected to a first bevel gear.

[0022] Beneficial effects: The dual-head drive unit synchronously drives the turbine blades and the first bevel gear. The turbine blades realize seawater suction, and the first bevel gear provides power for the subsequent stirring and dispensing components. A single power source realizes multi-stage linkage, simplifies the structure and reduces energy consumption.

[0023] Furthermore, the dispersing assembly includes a cavity opened in the side wall of the water inlet tank, a rotating shaft rotatably fitted on the side wall of the cavity, a second bevel gear coaxially and fixedly connected to one end of the rotating shaft, and a wheel rotatably fitted to the other end of the rotating shaft; a crank is eccentrically hinged on the wheel, a piston rod is hinged to the end of the crank away from the wheel, and a piston is fixedly connected to the end of the piston rod away from the crank.

[0024] A piston cylinder is fixedly connected to the bottom wall of the cavity, and the piston and the inner side wall of the piston cylinder slide together; an inlet pipe and an outlet pipe are connected to the side wall of the piston cylinder, and a one-way valve is connected to both the inlet pipe and the outlet pipe; the inlet pipe is connected to the microbial storage tank, and the outlet pipe is connected to the water inlet tank.

[0025] Several electromagnets are embedded circumferentially at the end of the rotating shaft away from the second bevel gear, and several iron cores are embedded circumferentially inside the wheel disk.

[0026] A water quality sensor is fixedly connected to the bottom of the float. The controller is used to receive the water quality signal sent by the water quality sensor and control the electromagnet to open and close based on the water quality signal.

[0027] Beneficial effects: The rotating shaft is driven by bevel gear transmission, and the magnetic attraction between the electromagnet and the iron core enables the intermittent rotation of the wheel, which in turn drives the piston to reciprocate. The quantitative intake and discharge of the compound microbial community is controlled by a one-way valve. Combined with the signal feedback from the water quality sensor, the controller can accurately control the amount of compound microbial community to be added by adjusting the opening and closing frequency of the electromagnet, so as to achieve on-demand addition and reduce resource waste and ecological burden.

[0028] Furthermore, a second support rod is fixedly connected to the inner side wall of the channel, and a rotating rod is rotatably fitted on the second support rod. One end of the rotating rod is fixedly connected to the first bevel gear on the same axis, and the other end of the rotating rod extends into the water inlet tank and is circumferentially fixedly connected to several stirring rods.

[0029] Beneficial effects: The rotating rod transmits power to the stirring rod, which allows the complex microbial community to be fully mixed with seawater in the inlet tank, forming a uniform bacterial solution. This avoids excessively high local concentrations or uneven degradation caused by the aggregation of the complex microbial community. At the same time, the stirring process enhances the fluidity of the seawater, promotes the rapid flow of the mixture through the channel into the drainage tank and release it into the target area, and improves the efficiency of the release.

[0030] Furthermore, a solar panel is fixedly connected to the top of the floating plate, which is used to power the controller and the dual-head drive unit.

[0031] Beneficial effects: Solar power eliminates the need for external cables, making it suitable for applications in remote waters with seagrass beds, reducing energy costs and environmental impact; it also reduces the frequency of battery replacements, enhancing the autonomy and environmental friendliness of the equipment's long-term operation.

[0032] Furthermore, each stirring rod is equipped with a flow-deflecting element.

[0033] Beneficial effects: The turbulence-inducing component can enhance water flow disturbance, break the laminar flow state, and make the complex microbial community more fully mixed with seawater. This ensures that the concentration of complex microbial community in each unit volume of mixed solution is uniform, improves the consistency of the distribution of the community in the seagrass bed after subsequent deployment, and ensures the stability of the degradation effect.

[0034] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0035] Figure 1 This is an isometric view of the composite microbial community delivery device for pollutant degradation according to the present invention.

[0036] Figure 2This is a side cross-sectional view of the composite microbial community delivery device for pollutant degradation according to the present invention.

[0037] Figure 3 for Figure 2 Enlarged view of section A.

[0038] Figure 4 This is a frontal cross-sectional view of the disc in the composite microbial community dispensing device for pollutant degradation according to the present invention.

[0039] The reference numerals in the accompanying drawings of the instruction manual include: 1. Float; 2. Filter steel mesh; 3. Inlet tank; 4. Drainage tank; 5. Channel; 6. Microbial storage tank; 7. First support rod; 8. Waterproof tank; 9. Dual-head motor; 10. Turbine fan blade; 11. First bevel gear; 12. Rotating shaft; 13. Second bevel gear; 14. Wheel; 15. Crank; 16. Piston rod; 17. Piston cylinder; 18. Electromagnet; 19. Iron core; 20. Water quality sensor; 21. Second support rod; 22. Rotating rod; 23. Stirring rod; 24. Solar panel. Detailed Implementation

[0040] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0041] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0042] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0043] The following detailed description illustrates the specific implementation method:

[0044] Example: A composite microbial community for pollutant degradation, comprising functional microorganisms, auxiliary microorganisms, and a microbial carrier, in a mass ratio of 4:3:3.

[0045] The functional microorganisms include: Rhodobulb, Dethiobacillus, Sphingomonas, and Nitrifying Spirochetes, in a mass ratio of 3:2:3:2.

[0046] Functional microorganisms (Rhodobulb, Dethiobacillus, Sphingomonas, Nitrifying Helicobacter) achieve multi-dimensional degradation of pollutants through metabolic specialization:

[0047] Rhodopseudomonas and Sphingomonas act as core degrading bacteria, respectively producing enzyme systems such as alkane hydroxylase and oxygenase to target and decompose petroleum hydrocarbons (polycyclic aromatic hydrocarbons) and pesticide residues (organophosphates), converting complex organic matter into small molecule metabolites (organic acids, CO2).

[0048] Desulfurobacteria convert highly toxic sulfides in seagrass bed sediments into less toxic forms through dissimilatory sulfate reduction, and further convert them through biosorption and redox reactions (such as Cr). 6 (⁺ reduced to Cr³⁺) reduces the bioavailability of heavy metals;

[0049] Nitrifying spirilla bacteria work synergistically with denitrification (converting NH4⁺ into NO3⁻) to balance the excess nitrogen released during pollutant degradation and prevent eutrophication of water bodies.

[0050] The auxiliary microorganisms include Burkholderia, Bacteroides, Methyltrophuria, and Green Curvature, in a mass ratio of 3:2:3:2.

[0051] Helper microorganisms enhance the function and environmental adaptability of the microbial community through ecological interactions:

[0052] Burkholderia secretes plant growth regulators such as indoleacetic acid (IAA), which promotes the development of seagrass roots and expands the colonization area of ​​the rhizosphere microenvironment. At the same time, it produces siderophores to help functional microorganisms obtain iron to enhance enzyme activity.

[0053] Methyltrophic bacteria utilize small molecule methyl compounds (such as methanol and methylamine) produced by the degradation of functional microorganisms to avoid the accumulation of intermediate products that inhibit the bacterial community.

[0054] Bacteroides construct biofilms by secreting extracellular polysaccharides, encapsulating functional microorganisms and enhancing the adhesion of the microbial community to sediments.

[0055] As the dominant native bacteria of seagrass beds, *Cyclocarya paliurus* stabilizes the bacterial community structure through metabolic interactions, thereby enhancing the overall tolerance to high-salt and hypoxic environments.

[0056] The microbial carrier consists of zeolite, sodium alginate, trehalose, and xanthan gum in a mass ratio of 4:3:2:1.

[0057] The carrier ensures the activity and colonization efficiency of the microbial community through physicochemical processes:

[0058] Zeolite, with its porous structure, adsorbs pollutants (such as heavy metal ions and petroleum hydrocarbons), creating a localized high-concentration polluted microenvironment that improves the degradation efficiency of functional microorganisms. At the same time, it provides colonization sites for the microbial community, reducing the loss caused by water erosion.

[0059] Sodium alginate forms gel microspheres that encapsulate the bacterial community. By adjusting the porosity of the microspheres, it maintains an internal hypoxic microenvironment (adapted to the conditions of seagrass bed sediments) and slowly releases the bacterial community into the rhizosphere region.

[0060] Trehalose, as a cryoprotectant, maintains the stability of microbial cell structure and extends shelf life during the storage of complex microbial communities; it dissolves rapidly upon contact with water, providing a carbon source for the initial metabolism of the microbial community.

[0061] Xanthan gum increases the viscosity of the carrier, promotes the adhesion of the complex microbial community to seagrass roots and sediment particles, and improves the colonization success rate.

[0062] like Figure 1 As shown, a composite microbial community dispensing device for pollutant degradation includes a dispensing component for dispensing the composite microbial community.

[0063] like Figure 2 As shown, specifically, the deployment components include, from top to bottom, a float 1, a filter steel mesh 2, an inlet tank 3, and an outlet tank 4, all fixedly welded together. The inlet tank 3 and the outlet tank 4 are connected. A channel 5 is opened on the bottom wall of the inlet tank 3, which is connected to the outlet tank 4. The outlet tank 4 has a drain port at its bottom. A microbial storage tank 6 is fixedly connected to the float 1 by screws. The outlet tank 4 is equipped with a suction component for drawing seawater into the inlet tank 3.

[0064] The inlet tank 3 is equipped with a dissemination component for distributing the complex microbial community in the bacterial storage tank 6 to the seawater drawn into the inlet tank 3.

[0065] Combination Figure 1 and Figure 2As shown, seawater is taken from the target seagrass bed area or a nearby sea area in advance and sterilized by ultraviolet irradiation. At the same time, a compound microbial community is added to the community storage tank 6, and the sterilized seawater is added to the community storage tank 6 to form a bacterial solution. Several delivery components are placed on the sea surface of the seagrass bed area. The float 1 allows the delivery components to float on the water surface, and the bottom part of the float 1 will sink into the water. At this time, the suction component can draw the seawater to the inlet tank 3 and then discharge it through the outlet tank 4. The filter steel mesh 2 can effectively intercept larger suspended impurities in the seawater, such as seagrass debris, to prevent them from entering the equipment and causing blockage.

[0066] During the extraction process, when the seaweed bed surface becomes polluted, the delivery component can release a complex microbial community into the extracted seawater. After being mixed evenly with the seawater, it is discharged. Traditionally, directly applied microbial agents are easily affected by ocean currents and tides, causing them to drift and be lost, making it difficult to cover the seaweed root zone.

[0067] This equipment forms a uniform bacterial solution through forced stirring and then discharges it from the bottom. It can be directly deployed below the water surface, allowing the bacterial community to settle more stably and centrally on the surface of seagrass rhizosphere sediments. This significantly increases the probability of contact between the bacterial community and the contaminated target area and the uniformity of colonization.

[0068] like Figure 2 As shown, specifically, the suction assembly includes a controller and a first support rod 7 fixedly welded to the inner wall of the drainage tank 4. A waterproof tank 8 is fixedly welded to the first support rod 7. A dual-head drive component is bolted to the inner wall of the waterproof tank 8. The controller is used to control the rotation of the output shaft of the dual-head drive component. In this embodiment, the dual-head drive component is a dual-head motor 9. A solar panel 24 is fixedly bonded to the top of the float 1. The solar panel 24 is used to power the controller and the dual-head motor 9.

[0069] One end of the output shaft of the dual-head motor 9 passes through the side wall of the adjacent waterproof box 8 and is coaxially fixedly welded with a turbine fan blade 10. The other end of the output shaft of the dual-head motor 9 passes through the side wall of the adjacent waterproof box 8 and extends into the channel 5 and is coaxially fixedly welded with a first bevel gear 11.

[0070] Combination Figure 2As shown, when seawater needs to be pumped for bacterial colony release, the controller starts the dual-head motor 9. At this time, the dual-head motor 9 drives the turbine blades 10 to rotate. When the turbine blades 10 rotate, they can push the seawater in the drainage tank 4 to be continuously discharged from the bottom drain. At this time, the drainage tank 4 generates negative pressure, which can draw water from the inlet tank 3 into the drainage tank 4 through the channel 5. The inlet tank 3 then generates negative pressure, which can draw seawater from the outside into the inlet tank 3 through the filter steel mesh 2, thereby achieving the pumping effect. At the same time, the solar panel 24 provides power to the dual-head motor 9 and the controller, which can adapt to the application scenarios of seagrass beds in remote waters, reduce energy costs and environmental interference; at the same time, it reduces the frequency of battery replacement and improves the autonomy and environmental friendliness of the long-term operation of the equipment.

[0071] like Figure 2 and Figure 3 As shown, specifically, the dispersing assembly includes a cavity opened in the side wall of the water inlet tank 3. A rotating shaft 12 is rotatably fitted on the side wall of the cavity. A second bevel gear 13 is integrally formed on one end of the rotating shaft 12, and a wheel 14 is rotatably fitted on the other end of the rotating shaft 12. A crank 15 is eccentrically hinged on the wheel 14. A piston rod 16 is hinged to the end of the crank 15 away from the wheel 14. A piston is integrally formed on the end of the piston rod 16 away from the crank 15.

[0072] A piston cylinder 17 is fixedly connected to the bottom wall of the cavity by screws, and the piston and the inner side wall of the piston cylinder 17 slide together; an inlet pipe and an outlet pipe are connected to the side wall of the piston cylinder 17, and a one-way valve is connected to both the inlet pipe and the outlet pipe; the inlet pipe is connected to the bacterial storage tank 6, and the outlet pipe is connected to the water inlet tank 3.

[0073] like Figure 4 As shown, a number of electromagnets 18 are embedded in the circumference of the end of the rotating shaft 12 away from the second bevel gear 13, and a number of iron cores 19 are embedded in the inner circumference of the wheel 14; a water quality sensor 20 is fixedly connected to the bottom of the float 1, and the controller is used to receive the water quality signal sent by the water quality sensor 20 and control the electromagnets 18 to open and close based on the water quality signal.

[0074] Combination Figure 2 As shown, in this embodiment, the water quality sensor 20 is existing technology. Its principle is to use a fluorescence spectral probe and a laser emitter. The laser emitter emits excitation light, and organic pollutants in seawater (such as polycyclic aromatic hydrocarbons as the main pollutants) will emit fluorescence after being irradiated by the excitation light. The fluorescence spectral probe receives the intensity of the fluorescence and then determines the concentration of pollutants in the seawater. This embodiment will not elaborate further.

[0075] Combination Figure 3 and Figure 4As shown, a pollutant concentration threshold is set for the controller. When the concentration of seawater pollutants in the environment surrounding the seagrass bed is greater than or equal to the concentration threshold, the controller starts the dual-head motor 9 and the electromagnet 18 to be energized. At this time, the electromagnet 18 can attract the iron core 19 on the wheel 14, so that the rotating shaft 12 and the wheel 14 become rigidly connected. At this time, the rotation of the rotating shaft 12 can drive the wheel 14 to rotate.

[0076] Because the first bevel gear 11 and the second bevel gear 13 mesh, the output shaft of the dual-head motor 9 rotates, driving the first bevel gear 11 to rotate, which in turn drives the second bevel gear 13 to rotate. The second bevel gear 13 then drives the rotating shaft 12 to rotate, which in turn drives the wheel 14 to rotate. The rotation of the wheel 14 then drives the eccentrically hinged crank 15, which in turn drives the piston rod 16, which in turn drives the piston to reciprocate up and down inside the piston cylinder 17. When the piston moves upward, the space inside the piston cylinder 17 increases, generating negative pressure. Under the action of the one-way valve in the inlet pipe, the bacterial storage tank 6 is filled with liquid. The compound microbial community solution is drawn into the piston cylinder 17. When the piston moves downward, the space inside the piston cylinder 17 decreases, generating positive pressure. Under the action of the one-way valve in the outlet pipe, the compound microbial community solution in the piston cylinder 17 can be pumped into the inlet tank 3 to mix with seawater, realizing the release of the microbial community. At the same time, after a certain period of microbial community release, the controller controls the electromagnet 18 to be de-energized. At this time, the electromagnet 18 releases the iron core 19, and the rotation of the shaft 12 can no longer drive the wheel 14 to rotate. The piston stops reciprocating, and the microbial community release is paused. By adjusting the time interval between the on and off of the electromagnet 18 and the duration of each energization, the amount of microbial community released can be controlled, thereby adjusting the concentration of the mixed microbial solution. Specifically:

[0077] When the water quality sensor 20 detects that the pollutant concentration is much higher than the threshold, the controller shortens the de-energization interval of the electromagnet 18 (e.g., energizing for 5 seconds every 10 seconds), prolongs the single-use time, increases the amount of bacteria added per unit time, and improves the degradation efficiency.

[0078] When the concentration of pollutants is detected to be close to the threshold, the controller extends the de-energization interval of the electromagnet 18 (e.g., energizing for 3 seconds every 30 seconds) to reduce the amount of bacteria to be released at one time and avoid resource waste caused by excessive release of bacteria; thus achieving on-demand release.

[0079] This intermittent microbial release logic can dynamically link microbial release with real-time pollution concentration, ensuring degradation intensity under high pollution conditions while avoiding ineffective consumption of microbial populations under low or no pollution conditions, thus significantly improving microbial utilization efficiency. At the same time, intermittent release also allows the microbial population to mix with seawater in the inlet tank 3 for a certain period of time, improving the uniformity of mixing between the microbial population and seawater.

[0080] like Figure 2As shown in the figure, a second support rod 21 is fixedly welded on the inner side wall of the channel 5. A rotating rod 22 is rotatably fitted on the second support rod 21. One end of the rotating rod 22 is fixedly welded coaxially with the first bevel gear 11, and the other end of the rotating rod 22 extends into the water inlet tank 3 and is fixedly welded circumferentially with a plurality of stirring rods 23. Turbulence components are provided on the stirring rods 23. In this embodiment, the turbulence components are stirring blades that are all rotatably fitted at one end of the stirring rod 23 away from the rotating rod 22.

[0081] When the microbial community is put in, the rotating rod 22 transmits power to the stirring rod 23, so that the composite microbial community and seawater are fully mixed in the water inlet tank 3 to form a uniform bacterial liquid, avoiding excessive local concentration or uneven degradation caused by the aggregation of the composite microbial community; the stirring process simultaneously enhances the fluidity of the seawater, promotes the mixed liquid to quickly pass through the channel 5 into the drainage tank 4 and be released to the target area, improving the putting efficiency. The turbulence components can enhance the water flow disturbance, break the laminar flow state, make the mixing of the composite microbial community and seawater more sufficient, ensure the uniformity of the concentration of the composite microbial community in each unit volume of the mixed liquid, improve the distribution consistency of the microbial community in the seagrass bed after subsequent putting, and ensure the stability of the degradation effect.

[0082] This solution realizes the synergistic degradation of various pollutants such as petroleum hydrocarbons, pesticide residues, and heavy metals through the targeted compatibility of functional microorganisms, breaking through the limitation that the existing microbial agents only focus on promoting seagrass growth and have a single pollutant degradation ability, and can specifically solve the problem of complex pollution in seagrass beds.

[0083] At the same time, the putting equipment supporting this solution can adaptively adjust the putting of the composite microbial community according to the pollution degree, improve the colonization efficiency of the composite microbial community in the rhizosphere area of seagrass, reduce ineffective loss, and is more efficient than the traditional manual spreading.

[0084] Obviously, the above embodiments are only examples given for clear illustration, and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation manners here. And the obvious changes or modifications derived therefrom are still within the protection scope of this invention.

Claims

1. A composite microbial community for pollutant degradation, characterized in that, It includes functional microorganisms, auxiliary microorganisms, and microbial carriers, with a mass ratio of 4:3:

3.

2. The composite microbial community for pollutant degradation according to claim 1, characterized in that, The functional microorganisms include: Rhodobulb, Dethiobacillus, Sphingomonas, and Nitrifying Spirochetes, in a mass ratio of 3:2:3:

2.

3. The composite microbial community for pollutant degradation according to claim 2, characterized in that, The auxiliary microorganisms include Burkholderia, Bacteroides, Methyltrophuria, and Green Curvature, in a mass ratio of 3:2:3:

2.

4. The composite microbial community for pollutant degradation according to claim 3, characterized in that, The microbial carrier consists of zeolite, sodium alginate, trehalose, and xanthan gum in a mass ratio of 4:3:2:

1.

5. A composite microbial community dispensing device for pollutant degradation, comprising any one of the composite microbial communities for pollutant degradation according to claims 1-4, characterized in that, Includes delivery components for distributing complex microbial communities; The delivery components include a float (1), a filter steel mesh (2), an inlet tank (3), and a drain tank (4) that are fixedly connected from top to bottom; the inlet tank (3) and the drain tank (4) are connected; a channel (5) is opened on the bottom wall of the inlet tank (3), the channel (5) is connected to the drain tank (4), and a drain outlet is opened at the bottom of the drain tank (4); A microbial storage box (6) is fixedly connected to the floating plate (1); the drainage box (4) is equipped with a suction component for pumping seawater into the inlet box (3); The inlet tank (3) is equipped with a dissemination component for distributing the complex microbial community in the bacterial storage tank (6) to the seawater pumped into the inlet tank (3).

6. The composite microbial community dispensing device for pollutant degradation according to claim 5, characterized in that, The suction assembly includes a controller and a first support rod (7) fixedly connected to the inner wall of the drain tank (4). A waterproof tank (8) is fixedly connected to the first support rod (7). A double-headed drive is fixedly connected to the inner wall of the waterproof tank (8). The controller is used to control the rotation of the output shaft of the double-headed drive. One end of the output shaft of the dual-head drive unit passes through the side wall of the adjacent waterproof box (8) and is coaxially fixedly connected to the turbine fan blade (10). The other end of the output shaft of the dual-head drive unit passes through the side wall of the adjacent waterproof box (8) and extends into the channel (5) and is coaxially fixedly connected to the first bevel gear (11).

7. The composite microbial community dispensing device for pollutant degradation according to claim 6, characterized in that, The dispersing assembly includes a cavity opened in the side wall of the water inlet tank (3), a rotating shaft (12) is rotatably fitted on the side wall of the cavity, a second bevel gear (13) is coaxially fixedly connected to one end of the rotating shaft (12), and a wheel (14) is rotatably fitted to the other end of the rotating shaft (12); a crank (15) is eccentrically hinged on the wheel (14), a piston rod (16) is hinged to the end of the crank (15) away from the wheel (14), and a piston is fixedly connected to the end of the piston rod (16) away from the crank (15); A piston cylinder (17) is fixedly connected to the bottom wall of the cavity, and the piston and the inner side wall of the piston cylinder (17) slide together; an inlet pipe and an outlet pipe are connected to the side wall of the piston cylinder (17), and a one-way valve is connected to both the inlet pipe and the outlet pipe; the inlet pipe is connected to the microbial storage box (6), and the outlet pipe is connected to the water inlet box (3); Several electromagnets (18) are embedded in the circumference of the shaft (12) away from the second bevel gear (13), and several iron cores (19) are embedded in the inner circumference of the wheel (14). A water quality sensor (20) is fixedly connected to the bottom of the float (1). The controller is used to receive the water quality signal sent by the water quality sensor (20) and control the electromagnet (18) to open and close based on the water quality signal.

8. The composite microbial community dispensing device for pollutant degradation according to claim 7, characterized in that, A second support rod (21) is fixedly connected to the inner wall of the channel (5). A rotating rod (22) is rotatably fitted on the second support rod (21). One end of the rotating rod (22) is coaxially fixedly connected to the first bevel gear (11). The other end of the rotating rod (22) extends into the water inlet tank (3) and is circumferentially fixedly connected to several stirring rods (23).

9. The composite microbial community dispensing device for pollutant degradation according to claim 8, characterized in that, A solar panel (24) is fixedly connected to the top of the floating plate (1), which is used to power the controller and the dual-head drive unit.

10. The composite microbial community dispensing device for pollutant degradation according to claim 9, characterized in that, All stirring rods (23) are equipped with turbulence-inducing components.

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