Capillary electrophoresis system and automatic capillary cleaning method
By designing a cleaning solution reservoir, gel reservoir, switching valve, and injection device in the capillary electrophoresis system, automated capillary cleaning was achieved, solving the problems of inconvenience and incomplete cleaning in existing technologies, and improving cleaning efficiency and effectiveness.
Patent Information
- Application Number
- CN202511915097.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-18
- Publication Date
- 2026-03-06
AI Technical Summary
The existing capillary electrophoresis system requires manual cleaning, which is often incomplete and results in poor cleaning effectiveness.
A capillary electrophoresis system was designed, comprising a cleaning solution reservoir, a gel reservoir, a switching valve, a liquid injection device, a capillary assembly, and a cathode cup. The automatic cleaning of the capillary is achieved by controlling the switching valve, and different liquids are delivered to the capillary assembly for cleaning by the liquid injection device.
It enables automated cleaning of capillaries, improving cleaning efficiency and effectiveness while reducing the hassle of manual operation.
Smart Images
Figure CN121612966A_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of capillary electrophoresis technology, and more specifically, to a capillary electrophoresis system and an automatic capillary cleaning method. Background Technology
[0002] In capillary electrophoresis systems, capillaries are typically managed as consumables. Their lifespan, number of uses, and daily maintenance are all closely related. There are daily cleaning plans, as well as monthly and weekly cleaning plans, all of which require cleaning operations.
[0003] The cleaning process of existing capillary electrophoresis systems is generally done manually, which is not only troublesome but also prone to incomplete cleaning.
[0004] Therefore, there is an urgent need for a capillary electrophoresis system and a method for automatic capillary cleaning that can achieve automatic capillary cleaning. Summary of the Invention
[0005] The purpose of this application is to provide a capillary electrophoresis system and a method for automatic capillary cleaning that can achieve automatic capillary cleaning.
[0006] To achieve the above objectives, this application provides a capillary electrophoresis system, including a cleaning solution reservoir, a gel reservoir, a switching valve, a liquid injection device, a capillary assembly, and a cathode cup. The cleaning solution reservoir stores capillary cleaning solution, the gel reservoir stores capillary gel, the switching valve is connected to the cleaning solution reservoir, the gel reservoir, and the liquid injection device, respectively, the liquid injection device is connected to the capillary assembly, and the capillary assembly is connected to the cathode cup. The switching valve is used to selectively connect either the cleaning solution reservoir or the gel reservoir to the liquid injection device. The liquid injection device can be used to deliver the cleaning solution stored in the cleaning solution reservoir to the capillary assembly when connected to the cleaning solution reservoir, and can also be used to deliver the cleaning solution stored in the cleaning solution reservoir to the capillary assembly when connected to the gel reservoir.
[0007] In some embodiments, multiple cleaning fluid reservoirs are provided, each of which is connected to the switching valve, and the multiple cleaning fluid reservoirs are used to store different capillary cleaning fluids.
[0008] In some embodiments, the capillary electrophoresis system further includes a first liquid path, one end of which is connected to the cleaning solution reservoir, and the other end of which is connected to the switching valve; and / or, The capillary electrophoresis system further includes a second liquid path, one end of which is connected to the gel reservoir and the other end of which is connected to the switching valve.
[0009] In some embodiments, the capillary electrophoresis system includes an anode cup, and the liquid injection device is connected to the anode cup, wherein the liquid injection device can discharge internal waste liquid into the anode cup.
[0010] In some embodiments, the capillary electrophoresis system further includes a draining device connected to the anode cup, which is used to extract waste liquid from the anode cup.
[0011] In some embodiments, the capillary electrophoresis system further includes a waste liquid storage device connected to the drainage device for discharging the waste liquid into the waste liquid storage device.
[0012] In some embodiments, the capillary electrophoresis system further includes a third liquid path, one end of which is connected to the draining device and the other end of which is connected to the waste liquid storage tank.
[0013] In some embodiments, the capillary electrophoresis system further includes a buffer reservoir for storing buffer solution, the buffer reservoir being connected to the switching valve for selectively connecting the buffer reservoir to the injection device, the injection device being used to deliver the buffer solution stored in the buffer reservoir to the anode cup when connected to the buffer reservoir.
[0014] To achieve the above objectives, this application provides an automatic capillary cleaning method applicable to the capillary electrophoresis system, the automatic capillary cleaning method comprising: The switching valve is controlled to switch the cleaning fluid reservoir to be connected to the injection device. The injection device draws the capillary cleaning fluid stored in the cleaning fluid reservoir and delivers the capillary cleaning fluid to the capillary assembly to clean the capillary assembly. The switching valve is controlled to connect the gel reservoir to the injection device, the injection device draws the capillary gel stored in the gel reservoir and delivers the capillary gel to the capillary assembly.
[0015] To achieve the above objectives, this application provides an automatic capillary cleaning method applicable to the capillary electrophoresis system, the automatic capillary cleaning method comprising: The liquid injection device empties the liquid stored inside and delivers it to the anode cup, and the liquid drainage device extracts the liquid from the anode cup. The switching valve is controlled to switch the cleaning fluid reservoir to be connected to the injection device. The injection device draws the capillary cleaning fluid stored in the cleaning fluid reservoir and delivers the capillary cleaning fluid to the capillary assembly to clean the capillary assembly. The switching valve is controlled to connect the buffer reservoir to the injection device, and the injection device draws the buffer stored in the buffer reservoir and delivers the buffer to the anode cup; The switching valve is controlled to connect the gel reservoir to the injection device, the injection device draws the capillary gel stored in the gel reservoir and delivers the capillary gel to the capillary assembly.
[0016] Compared with the prior art, the capillary electrophoresis system of this application includes a cleaning solution reservoir, a gel reservoir, a switching valve, a liquid injection device, a capillary assembly, and a cathode cup. The cleaning solution reservoir stores capillary cleaning solution, the gel reservoir stores capillary gel, the switching valve is connected to the cleaning solution reservoir, the gel reservoir, and the liquid injection device, respectively, the liquid injection device is connected to the capillary assembly, and the capillary assembly is connected to the cathode cup. The switching valve is used to selectively connect either the cleaning solution reservoir or the gel reservoir to the liquid injection device. The liquid injection device can be used to deliver the cleaning solution stored in the cleaning solution reservoir to the capillary assembly when connected to the cleaning solution reservoir, and can also be used to deliver the cleaning solution stored in the cleaning solution reservoir to the capillary assembly when connected to the gel reservoir. This allows the capillary electrophoresis system of this application to achieve automatic capillary cleaning. Similarly, the automatic capillary cleaning method of this application can achieve automatic capillary cleaning. Attached Figure Description
[0017] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments of this application will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.
[0018] Figure 1 This is a schematic diagram of a capillary electrophoresis system according to an embodiment of this application.
[0019] Figure 2 This is a flowchart of an automatic capillary cleaning method according to an embodiment of this application. Detailed Implementation
[0020] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings.
[0021] In the description of this application, it should be noted that the terms "inner" and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use. They are used only for the convenience of describing this application and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.
[0022] It should also be noted that, unless otherwise explicitly specified and limited, the terms "setup" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.
[0023] Please see Figure 1 The capillary electrophoresis system 100 of this application embodiment includes a high-voltage power supply 10, a detector 20, a cleaning solution reservoir 31, a gel reservoir 32, a switching valve 40, a liquid injection device 50, a capillary assembly 60, and a cathode cup 71. The cleaning solution reservoir 31 is used to store capillary cleaning solution, and the gel reservoir 32 is used to store capillary gel. The switching valve 40 is connected to the cleaning solution reservoir 31, the gel reservoir 32, and the liquid injection device 50, respectively. The liquid injection device 50 is connected to the capillary assembly 60, and the capillary assembly 60 is connected to the cathode cup 71. The switching valve 40 is used to selectively connect the cleaning solution reservoir 31 or the gel reservoir 32 to the liquid injection device 50. The liquid injection device 50 can be used to deliver the cleaning solution stored in the cleaning solution reservoir 31 to the capillary assembly 60 when connected to the cleaning solution reservoir 31, and can also be used to deliver the cleaning solution stored in the cleaning solution reservoir 31 to the capillary assembly 60 when connected to the gel reservoir 32. More specifically, as follows: like Figure 1 As shown, in some embodiments, multiple cleaning fluid reservoirs 31 are provided, and the multiple cleaning fluid reservoirs 31 are respectively connected to the switching valve 40. The multiple cleaning fluid reservoirs 31 are used to store different capillary cleaning fluids.
[0024] like Figure 1As shown, in some embodiments, the capillary electrophoresis system 100 further includes a first liquid passage 91, one end of which is connected to the cleaning liquid reservoir 31, and the other end of which is connected to the switching valve 40, so that the first liquid passage 91 is in communication with the switching valve 40.
[0025] like Figure 1 As shown, in some embodiments, the capillary electrophoresis system 100 further includes a second liquid passage 92, one end of which is connected to the gel reservoir 32, and the other end of which is connected to the switching valve 40, so that the second liquid passage 92 is in communication with the switching valve 40.
[0026] In some embodiments, the capillary electrophoresis system 100 includes an anode cup 72, and a liquid injection device 50 is connected to the anode cup 72. The liquid injection device 50 can discharge waste liquid into the anode cup 72.
[0027] like Figure 1 As shown, in some embodiments, the capillary electrophoresis system 100 further includes a draining device 81, which is connected to the anode cup 72 so that the draining device 81 can extract the waste liquid in the anode cup 72.
[0028] like Figure 1 As shown, in some embodiments, the capillary electrophoresis system 100 further includes a waste liquid reservoir 82, which is connected to a drain device 81 so that the drain device 81 can discharge waste liquid to the waste liquid reservoir 82.
[0029] like Figure 1 As shown, in some embodiments, the capillary electrophoresis system 100 further includes a third liquid passage 93, one end of which is connected to the drain device 81, and the other end of which is connected to the waste liquid storage device 82, so that the third liquid passage 93 is in communication with the waste liquid storage device 82.
[0030] like Figure 1 As shown, in some embodiments, the capillary electrophoresis system 100 further includes a buffer reservoir 33 for storing buffer solution. The buffer reservoir 33 is connected to a switching valve 40, which is used to selectively connect the buffer reservoir 33 to a dispensing device 50. The dispensing device 50 can be used to deliver the buffer solution stored in the buffer reservoir 33 to the anode cup 72 when connected to the buffer reservoir 33.
[0031] like Figure 2 As shown, the automatic capillary cleaning method of this application includes: The liquid injection device 50 empties the liquid stored inside and delivers it to the anode cup 72, while the liquid drainage device 81 extracts the liquid from the anode cup 72. The control switching valve 40 is switched to connect the cleaning fluid reservoir 31 to the injection device 50. The injection device 50 draws the capillary cleaning fluid stored in the cleaning fluid reservoir 31 and delivers the capillary cleaning fluid to the capillary assembly 60 to clean the capillary assembly 60. The control switching valve 40 is switched to connect the buffer storage tank 33 to the injection device 50. The injection device 50 draws the buffer stored in the buffer storage tank 33 and delivers the buffer to the anode cup 72. The control switching valve 40 switches to connect the gel reservoir 32 to the injection device 50. The injection device 50 draws the capillary gel stored in the gel reservoir 32 and delivers the capillary gel to the capillary assembly 60.
[0032] Compared with the prior art, the capillary electrophoresis system 100 of this application embodiment includes a cleaning solution reservoir 31, a gel reservoir 32, a switching valve 40, a liquid injection device 50, a capillary assembly 60, and a cathode cup 71; the cleaning solution reservoir 31 is used to store capillary cleaning solution, the gel reservoir 32 is used to store capillary gel, the switching valve 40 is connected to the cleaning solution reservoir 31, the gel reservoir 32, and the liquid injection device 50 respectively, the liquid injection device 50 is connected to the capillary assembly 60, and the capillary assembly 60 is connected to the cathode cup. 71. The switching valve 40 is used to selectively connect the cleaning solution reservoir 31 or the gel reservoir 32 to the injection device 50. The injection device 50 can be used to deliver the cleaning solution stored in the cleaning solution reservoir 31 to the capillary assembly 60 when connected to the cleaning solution reservoir 31. The injection device 50 can also be used to deliver the cleaning solution stored in the cleaning solution reservoir 31 to the capillary assembly 60 when connected to the gel reservoir 32, enabling the capillary electrophoresis system 100 of this embodiment to achieve automatic capillary cleaning. Similarly, the automatic capillary cleaning method of this embodiment can achieve automatic capillary cleaning.
[0033] The above description is merely an embodiment of this application and is not intended to limit the scope of protection of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of protection of this application.
Claims
1. A capillary electrophoresis system characterized by, The capillary electrophoresis system comprises a cleaning liquid reservoir, a gel reservoir, a switching valve, a liquid injection device, a capillary assembly and a cathode cup; the cleaning liquid reservoir is used for storing capillary cleaning liquid, the gel reservoir is used for storing capillary gel, the switching valve is in communication with the cleaning liquid reservoir, the gel reservoir and the liquid injection device respectively, the liquid injection device is in communication with the capillary assembly, the capillary assembly is connected with the cathode cup, the switching valve is used for selectively connecting the cleaning liquid reservoir or the gel reservoir with the liquid injection device, and the liquid injection device is used for conveying the cleaning liquid stored in the cleaning liquid reservoir into the capillary assembly when the cleaning liquid reservoir is connected with the liquid injection device.
2. The capillary electrophoresis system of claim 1, wherein, The capillary electrophoresis system comprises a plurality of cleaning liquid reservoirs, and the plurality of cleaning liquid reservoirs are in communication with the switching valve respectively, and the plurality of cleaning liquid reservoirs are used for storing different capillary cleaning liquids.
3. The capillary electrophoresis system of claim 1, wherein, The capillary electrophoresis system further comprises a first liquid path pipe, one end of the first liquid path pipe is connected with the cleaning liquid reservoir, and the other end of the first liquid path pipe is connected with the switching valve. And / or, The capillary electrophoresis system further comprises a second liquid path pipe, one end of the second liquid path pipe is connected with the gel reservoir, and the other end of the second liquid path pipe is connected with the switching valve.
4. The capillary electrophoresis system of claim 1, wherein, The capillary electrophoresis system comprises an anode cup, the liquid injection device is in communication with the anode cup, and the liquid injection device can discharge the waste liquid in the interior to the anode cup.
5. The capillary electrophoresis system of claim 4, wherein, The capillary electrophoresis system further comprises a liquid discharge device, the liquid discharge device is in communication with the anode cup, and the liquid discharge device is used for discharging the waste liquid in the anode cup out of the anode cup.
6. The capillary electrophoresis system of claim 5, wherein, The capillary electrophoresis system further comprises a waste liquid reservoir, the waste liquid reservoir is in communication with the liquid discharge device, and the liquid discharge device is used for discharging the waste liquid to the waste liquid reservoir.
7. The capillary electrophoresis system of claim 5, wherein, The capillary electrophoresis system further comprises a third liquid path pipe, one end of the third liquid path pipe is connected with the liquid discharge device, and the other end of the third liquid path pipe is connected with the waste liquid reservoir.
8. The capillary electrophoresis system of claim 5, wherein, The capillary electrophoresis system further comprises a buffer liquid reservoir, the buffer liquid reservoir is used for storing buffer liquid, the buffer liquid reservoir is in communication with the switching valve, the switching valve is used for selectively connecting the buffer liquid reservoir with the liquid injection device, and the liquid injection device is used for conveying the buffer liquid stored in the buffer liquid reservoir into the anode cup when the buffer liquid reservoir is connected with the liquid injection device.
9. A method for automatic cleaning of a capillary, suitable for use in a capillary electrophoresis system as claimed in any one of the claims 1-8, characterized in that, The capillary automatic cleaning method comprises: controlling the switching valve to switch to the cleaning liquid reservoir in communication with the liquid injection device, the liquid injection device extracting the capillary cleaning liquid stored in the cleaning liquid reservoir and conveying the capillary cleaning liquid into the capillary assembly to clean the capillary assembly; controlling the switching valve to switch to the gel reservoir in communication with the liquid injection device, the liquid injection device extracting the capillary gel stored in the gel reservoir and conveying the capillary gel into the capillary assembly.
10. A method for automatic cleaning of a capillary, suitable for use in a capillary electrophoresis system as claimed in any one of claims 5-8, characterized in that, The capillary automatic cleaning method comprises: The liquid injection device empties the liquid stored therein and delivers the liquid into the anode cup, and the liquid discharge device draws the liquid in the anode cup out of the anode cup; The switching valve is controlled to switch to communication between the cleaning liquid reservoir and the liquid injection device, the liquid injection device draws the capillary cleaning liquid stored in the cleaning liquid reservoir, and delivers the capillary cleaning liquid into the capillary assembly to clean the capillary assembly; The switching valve is controlled to switch to communication between the buffer liquid reservoir and the liquid injection device, the liquid injection device draws the buffer liquid stored in the buffer liquid reservoir, and delivers the buffer liquid into the anode cup; The switching valve is controlled to switch to communication between the gel reservoir and the liquid injection device, the liquid injection device draws the capillary gel stored in the gel reservoir, and delivers the capillary gel into the capillary assembly.