Method for detecting concentration of acetic acid in anaerobic fermentation system by using bioelectrochemical sensor

By using screen-printed electrodes modified with reduced graphene oxide and polymethylene blue in a bioelectrochemical sensor, and combining the action of enzymes such as acetate kinase, the accuracy and range problems of existing sensors in anaerobic fermentation systems have been solved, achieving rapid and accurate measurement of acetic acid concentration, reducing costs and simplifying operation.

CN121633217APending Publication Date: 2026-03-10HUNAN UNIV
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Patent Information

Application Number
CN202411235616.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-09-04
Publication Date
2026-03-10

AI Technical Summary

Technical Problem

Existing bioelectrochemical sensors suffer from narrow detection range and poor accuracy when detecting acetic acid concentration in anaerobic fermentation systems. In particular, they are difficult to accurately collect current signals under high acetic acid concentration conditions, resulting in inaccurate detection results.

Method used

A screen-printed electrode was used as the working electrode, modified with reduced graphene oxide and polymethylene blue, and connected with acetate kinase, pyruvate kinase and lactate dehydrogenase through a cross-linking agent to construct a bioelectrochemical sensor. Cyclic voltammetry was used for scanning and current detection, and a standard linear regression equation between acetic acid concentration and current was established.

Benefits of technology

The improved conductivity and enzyme loading area of ​​the sensor enable rapid and accurate detection of acetic acid concentration, resulting in better applicability. It can accurately detect acetic acid concentration under high concentration conditions, reduces detection costs, and simplifies the operation process.

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Abstract

The invention discloses a method for detecting the concentration of acetic acid in an anaerobic fermentation system by using a bioelectrochemical sensor, acetic acid is detected by using the bioelectrochemical sensor, the bioelectrochemical sensor comprises a screen-printed electrode, and the working surface of the screen-printed electrode is modified with reduced graphene oxide and polymethylene blue. The working surface of the screen printing electrode is connected with acetokinase, pyruvate kinase and lactic dehydrogenase through a cross-linking agent. According to the method, the adopted sensor has better conductivity, higher response speed and higher current intensity, when the sensor is used for detecting the concentration of acetic acid in a fermentation system, the sensor has the advantages of being easy to operate, low in sample consumption, low in price, high in accurate measurement precision, high in detection speed and the like, and rapid detection of the concentration of acetic acid can be achieved; the acetic acid concentration in the anaerobic fermentation process in different periods can be timely and accurately obtained, effective data can be provided for anaerobic fermentation analysis, and the method has important significance for monitoring the process stability of anaerobic fermentation.
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