Shell-imitated laver protonema culture membrane and preparation method thereof

Abstract

The present invention relates to a shell-like laver filament culture film which simulates a prismatic layer, a pearl layer, and a lime layer of a shell, and a preparation method therefor, the shell-like laver filament culture film according to one embodiment of the present invention being characterized by comprising: a base film layer; the filament growth layer is formed at the upper part of the base film layer and forms an environment in which laver filaments can permeate and grow; and a filament protection layer which is formed on the upper part of the filament growth layer, forms a plurality of micropores through which the laver filaments can pass, and protects the laver filaments growing in the filament growth layer from external influence.

Description

Technical Field

[0001] This invention relates to a culture membrane for seashell-like filamentous plants of Porphyra yezoensis and its preparation method. More specifically, it relates to a culture membrane for seashell-like filamentous plants of Porphyra yezoensis and its preparation method, which simulates the prismatic layer, nacreous layer, and lime layer of a seashell, and improves the penetration rate and growth of Porphyra yezoensis filaments through material and structural characteristics. Background Technology

[0002] The typical laver cultivation process consists of a series of steps. First, carpospores are collected from mature laver thallus and cultured in a culture container for about 2-3 months while adjusting the water temperature and light to obtain free filaments. Then, these free filaments are transplanted into oyster shells, allowing the filaments to penetrate the pores formed in the nacreous layer of the oyster shell to reproduce and form sporangia. At the optimal seed collection period, the oyster shells with sporangia are hung on seaweed rafts, allowing the spores contained in the sporangia to be released from the oyster shells by the current and waves and attach to the seaweed rafts, thus enabling the laver thallus to grow on the seaweed rafts.

[0003] In the existing laver farming, the cultivation method for filamentous organisms transplanted into the shells mainly adopts the following approach: oyster shells are arranged at the bottom of a wooden or plastic box or a concrete trough and filled with culture water, allowing the filamentous organisms transplanted into the shells to reproduce.

[0004] However, this method has problems such as difficulty in obtaining high-quality oyster shells, cumbersome oyster shell processing, and environmental pollution.

[0005] Furthermore, each oyster shell used for culturing filaments needs to be manually cleaned and arranged one by one at the bottom of the incubator or culture tank before transplanting the filaments. This requires a large amount of manpower, and the weight during transportation increases both transportation and labor costs. In addition, there is the extra step of placing the shells in a collection net and hanging them. When there is a large overlap, the number of seeds is not abundant relative to the cost, and the use of oyster shells and collection nets after a single use causes environmental pollution.

[0006] Therefore, in order to solve this problem, the applicant disclosed Korean Patent No. 10-2629468, "Filament Culture Membrane for Laver Cultivation and Filament Culture Method Thereof".

[0007] The applicant's Korean patent relates to a culture membrane and a method for effectively culturing filaments for seaweed cultivation. The culture membrane includes: a base membrane formed of a rectangle with a specified width; and a coating base membrane formed of a coating film formed on the upper surface of the base membrane. The base membrane and the coating film are made of biodegradable materials. The advantage is that it solves the supply and demand problem of raw materials by replacing oyster shells, which were previously difficult to supply and process. Since there is no burden of labor costs caused by cleaning and arranging oyster shells, the manufacturing cost is low. Furthermore, it is easy to transport due to its excellent lightweight properties and does not cause environmental pollution.

[0008] On the other hand, in order to use filament culture membranes to culture filaments more effectively, the filament culture membranes should be as similar as possible to the filament culture structure of oyster shells, with a high filament penetration rate, and ensure sufficient space for filament culture.

[0009] Therefore, there is a further need for a filament culture structure that is closer to an oyster shell than the existing filament culture membranes used for laver cultivation, and a method that can improve the penetration rate of filaments and ensure sufficient culture space for filaments, so as to effectively cultivate laver filaments. Summary of the Invention

[0010] This invention is proposed to solve the above-mentioned problems. The purpose of this invention is to provide a shell-like culture membrane for seaweed filaments and its preparation method. It is designed to closely resemble an oyster shell, which consists of a lime layer that prevents filaments from penetrating, a pearl layer that allows filaments to grow, and a prism layer that protects the filaments. The membrane is formed by three structures: a base membrane layer that prevents filaments from penetrating, a filament growth layer that forms the growth environment for filaments, and a filament protective layer that protects the filaments. The material and structural characteristics of each layer are used to improve the penetration rate and growth of seaweed filaments.

[0011] To achieve the above objectives, the present invention provides a biomimetic seaweed filament culture membrane, comprising: a base membrane layer composed of a biodegradable material; a filament growth layer formed on the upper part of the base membrane layer, forming an environment that allows seaweed filaments to penetrate and grow; and a filament protection layer formed on the upper part of the filament growth layer, forming a plurality of micropores that allow the seaweed filaments to pass through, protecting the seaweed filaments growing in the filament growth layer from external influences.

[0012] In a preferred embodiment, the present invention is characterized in that the filamentous growth layer is composed of a porous sheet made of a mixture or reaction of a hydrophilic material and a biomaterial that provides nutrients to the laver filaments.

[0013] Furthermore, the present invention is characterized in that the biological material is one or more of the following powders or liquids: agar, pearl, glucose, white sugar, yeast, chitosan, hyaluronic acid, vitamin A, vitamin E, vitamin K, calcium carbonate, sodium nitrate, calcium phosphate, potassium phosphate, sodium sulfate, calcium hydroxide, and magnesium hydroxide.

[0014] Furthermore, the present invention is characterized in that the filamentous protective layer is formed by adding an antibacterial and deodorizing agent to a hydrophilic polymer membrane.

[0015] Furthermore, the present invention is characterized in that the filamentous protective layer has a plurality of protrusions arranged longitudinally and laterally at intervals on its lower surface facing the filamentous growth layer, and is engaged with the filamentous growth layer through the plurality of protrusions.

[0016] Furthermore, the base film layer, the filamentous growth layer, and the filamentous protective layer are each composed of one or more of the following: polybutylene adipate terephthalate (PBAT), polybutylene succinate (PBS), polyhydroxyalkanoate (PHA), polylactic acid (PLA), polycaprolactone (PCL), polyvinyl alcohol (PVA), polyglycolic acid (PGA), modified cellulose, and starch-modified resin. The materials of the base film layer, the filamentous growth layer, and the filamentous protective layer may be the same or different from each other.

[0017] On the other hand, the present invention also provides a method for preparing a culture membrane for filamentous structures similar to those of *Porphyra yezoensis*, the method comprising the following steps: preparing a base membrane and a hydrophilic polymer membrane; generating a mixture by mixing a polyol and a biomaterial and then stirring; generating a reactant by adding an isocyanate to the mixture and stirring; pouring the reactant onto the hydrophilic polymer membrane, maintaining its shape to a predetermined thickness and then drying it; forming a porous sheet on the hydrophilic polymer membrane and bonding the hydrophilic polymer membrane to the porous sheet; subjecting the hydrophilic polymer membrane to physical or chemical treatment to form a predetermined pattern or micropores at equal intervals; and bonding the porous sheet to the base membrane.

[0018] The present invention has the following excellent effects.

[0019] First, the shell-like laver filament culture membrane of one embodiment of the present invention is a triple structure consisting of a base membrane layer that prevents filaments from penetrating, a filament growth layer that forms a filament growth environment, and a filament protective layer that protects the filaments. Therefore, it provides an environment that is as similar as possible in morphology to the oyster shells in which laver filaments can grow.

[0020] Furthermore, since the filamentous growth layer is composed of a hydrophilic porous sheet containing agar or pearl as nutrients for the filamentous seaweed, the seashell-like filamentous culture membrane of an embodiment of the present invention has the effect of improving the growth of the filamentous seaweed.

[0021] Furthermore, since the filamentous protective layer forms multiple pores that allow the seaweed filaments to pass through and reach the filamentous growth layer, the seashell-like seaweed filamentous culture membrane of an embodiment of the present invention has the effect of improving the penetration rate of seaweed filaments.

[0022] Furthermore, the filamentous protective layer and the filamentous growth layer are joined by multiple convex protrusions formed on the lower surface of the filamentous protective layer, thus improving the bonding force and providing ample cultivation space for the laver filaments.

[0023] On the other hand, the effects obtained from the present invention are not limited to those described above, and those skilled in the art to which this invention pertains can clearly understand other effects not mentioned from the following description. Attached Figure Description

[0024] Figure 1 A diagram showing the shell in magnified form to illustrate its structure.

[0025] Figure 2 A diagram illustrating the structure of the seashell-like porphyria filament culture membrane of the present invention.

[0026] Figure 3 A cross-sectional view is provided to illustrate the structure of the seashell-like porphyria filament culture membrane of the present invention.

[0027] Figure 4 To show in magnified form Figure 3 A diagram showing the relationship between the filamentous growth layer and the filamentous protective layer in a culture membrane for the filamentous structures of *Porphyra yezoensis*.

[0028] Figure 5 This is a flowchart of the preparation method of the seashell-like porphyria filament culture membrane of the present invention.

[0029] Explanation of reference numerals in the attached figures

[0030] 100: Imitation seashell-shaped filamentous culture membrane

[0031] 110: Base film layer

[0032] 120: Filament growth layer

[0033] 130: Filamentous protective layer

[0034] 132: Micropores

[0035] 135: Convex protrusion

[0036] 140: Filament Culture Space Detailed Implementation

[0037] The terminology used in this invention uses generally used terms that are currently widely used, but in certain cases, terms chosen arbitrarily by the applicant are used. In such cases, the meaning should be understood in light of the meaning recorded or used in the detailed description of the invention rather than simply the name of the term.

[0038] The technical structure of the present invention will now be described in detail with reference to the preferred embodiments shown in the accompanying drawings.

[0039] However, the invention is not limited to the embodiments described herein, and may be embodied in other forms. Throughout the specification, the same reference numerals denote the same structural elements.

[0040] Figure 1 A diagram showing the shell in magnified form to illustrate its structure.

[0041] observe Figure 1 The shell is composed of a limestone layer CL, a nacreous layer NL located above the limestone layer CL, and a prismatic layer PL located above the nacreous layer NL.

[0042] The prismatic layer PL of the shell is a dense structure composed of inorganic material and has pores, while the nacreous layer NL is a relatively loose structure composed of layers of calcium carbonate (CaCO3) crystals. Therefore, the laver filaments penetrate into the nacreous layer NL through the pores of the prismatic layer PL and grow in the nacreous layer NL by absorbing nutrients.

[0043] On the other hand, the lime layer CL is composed of lime, so it cannot allow the laver filaments to penetrate.

[0044] An embodiment of the present invention provides a seashell-like culture membrane for *Porphyra filaments* that simulates the structure of the aforementioned seashells, providing an environment as similar as possible in shape to the oyster shells that enable the growth of *Porphyra filaments*.

[0045] Figure 2 A diagram illustrating the structure of the seashell-like porphyria filament culture membrane of the present invention is provided. Figure 3 A cross-sectional view is provided to illustrate the structure of the seashell-like porphyria filament culture membrane of the present invention.

[0046] observe Figure 2and Figure 3 The seashell-like filament culture membrane 100 of one embodiment of the present invention is formed by a triple structure including a base membrane layer 110, a filament growth layer 120 and a filament protective layer 130.

[0047] Specifically, the base film layer 110 simulates the calcareous layer CL of a seashell and forms the bottom of the film 100. It is made of a biodegradable material and can be a soft, transparent or translucent material that allows light to pass through.

[0048] More specifically, the base film layer 110 is composed of one or more of the following biodegradable, soft, transparent or translucent polybutylene adipate, polybutylene succinate, polyhydroxyalkanoate, polylactic acid, polycaprolactone, cross-linked polyvinyl alcohol, polyglycolic acid, modified cellulose, and starch-modified resin.

[0049] The base film layer 110 is made of biodegradable material, so it can be landfilled when it is disposed of, and it will biodegrade within a few months after landfilling, thus not causing environmental pollution.

[0050] Furthermore, since the base film layer 110 is flexible, it can be prepared in roll form, which offers advantages in preparation and processing. Also, since it is made of a transparent or translucent material, it increases the transmittance of light reflected to the bottom of the culture tank and facilitates microscopic inspection under a microscope.

[0051] Preferably, the base film layer 110 has a specified thickness and may have the same shape as the culture tank, but this is not a necessary limitation. The thickness or shape may be changed according to the user's intention or the usage environment.

[0052] The filamentous growth layer 120, which mimics the nacreous layer NL of a seashell, is formed on the upper part of the basement membrane layer 110, creating an environment that allows the laver filaments to penetrate and grow.

[0053] Preferably, the filamentous growth layer 120 is composed of a porous sheet made of a mixture or reaction of a hydrophilic material and a biomaterial that provides nutrients to the laver filaments.

[0054] The biomaterial can be any material that can provide nutrients to the filamentous seaweed, but it can be, for example, one or more of the following: agar, pearl, glucose, sugar, yeast, chitosan, hyaluronic acid, vitamin A, vitamin E, vitamin K, calcium carbonate, sodium nitrate, calcium phosphate, potassium phosphate, sodium sulfate, calcium hydroxide, and magnesium hydroxide. It is mixed or reacted with the filamentous growth layer 120 in powder or liquid form and formed into a porous sheet.

[0055] The filamentous growth layer 120 may be made of a hydrophilic and biodegradable material, specifically, it may be one or more of the following: polybutylene adipate terephthalate, polybutylene succinate, polyhydroxyalkanoate, polylactic acid, polycaprolactone, cross-linked polyvinyl alcohol, polyglycolic acid, modified cellulose, and starch-modified resin.

[0056] That is, the filamentous growth layer 120 is formed by mixing or reacting a hydrophilic biodegradable material with the biological material. The filamentous growth layer 120 is composed of a porous or integral sheet with a specified thickness, and a specified space is left between it and the filamentous protective layer 130, so that the laver filaments can grow fully and can grow effectively by receiving nutrients from the biological material.

[0057] The filamentous growth layer 120 is also made of biodegradable material, so it can be landfilled when discarded and will biodegrade within a few months after landfilling, thus not causing environmental pollution. Furthermore, because it is soft, it can be prepared in roll form, which gives it advantages in preparation and treatment.

[0058] On the other hand, the filamentous growth layer 120 is configured to be made of the same or different material from the base film layer 110.

[0059] The filamentous protective layer 130, which mimics the prismatic layer PL of a seashell, is formed on the upper part of the filamentous growth layer 120. Therefore, it protects the laver filaments growing in the filamentous growth layer 120 from external influences and forms multiple micropores 132 through which the laver filaments can pass.

[0060] The micropores 132 are pores with a diameter of about 10 to 100 μm, which penetrate the filamentous protective layer 130 and extend into the filamentous growth layer 120, thus facilitating the easy passage and penetration of laver filaments from the outside into the filamentous growth layer 120.

[0061] The filamentous protective layer 130 has a specified thickness of 1 μm to 20 μm and is a biodegradable hydrophilic polymer membrane with micropores 132 formed by physical or chemical treatment or having a fibrous structure such as nonwoven fabric. It may be composed of one or more of the following: polybutylene adipate terephthalate, polybutylene succinate, polyhydroxyalkanoate, polylactic acid, polycaprolactone, cross-linked polyvinyl alcohol, polyglycolic acid, modified cellulose, and starch-modified resin.

[0062] Furthermore, the filamentous protective layer 130 is coated with antibacterial and deodorizing agents or treated with antibacterial agents to protect the laver filaments growing in the filamentous growth layer 120 from the influence of viruses and bacteria.

[0063] In the case of the filament protective layer 130, the same material as the base film layer 110 or the filament growth layer 120 may be used, or a different material may be used.

[0064] The filamentous protective layer 130 is also made of biodegradable material, so it can be landfilled when discarded and will biodegrade within a few months after landfilling, thus not causing environmental pollution. Furthermore, because it is soft, it can be prepared in roll form, which gives it advantages in preparation and treatment.

[0065] Furthermore, the filamentous protective layer 130 is light-transmitting, allowing light to easily reach the filamentous growth layer 120. Therefore, even under low illumination, seaweed filaments can be cultivated, and the possibility of diatoms can be reduced.

[0066] On the other hand, the filamentous protective layer 130 has a plurality of protrusions 135 arranged longitudinally and laterally on its lower surface facing the filamentous growth layer 120, and is engaged with the filamentous growth layer 120 through the protrusions 135.

[0067] Figure 4 To show in magnified form Figure 3 A diagram showing the relationship between the filamentous growth layer and the filamentous protective layer in a culture membrane for the filamentous structures of *Porphyra yezoensis*.

[0068] observe Figure 4 It can be seen that a plurality of protrusions 135 are formed on the lower surface of the filamentous protective layer 130, and the filamentous protective layer 130 is joined to the filamentous growth layer 120 through the plurality of protrusions 135.

[0069] The protrusions 135 are bonded to the filament growth layer 120 by means of adhesive or heating. As shown in the figure, the protrusions 135 not only improve the bonding force, but also form a plurality of filament culture spaces 140 that enable filament growth in the space between the protrusions 135 between the filament protective layer 130 and the filament growth layer 120.

[0070] As described above, the seashell-like porphyria filament culture membrane 100 of the present invention can ensure sufficient space for culturing porphyria filaments, and not only can it effectively cultivate porphyria filaments, but it also has excellent interlayer adhesion, thus preventing peeling and having excellent durability.

[0071] On the other hand, the spacing between the protrusions 135 can be less than 10 μm to 1 mm.

[0072] The following will refer to Figure 5 The preparation method of the above-mentioned shell-like porphyria filament culture membrane is explained.

[0073] Figure 5 This is a flowchart of the preparation method of the seashell-like porphyria filament culture membrane of the present invention.

[0074] observe Figure 5 In the preparation method of the seashell-like porphyria filament culture membrane of the present invention, although not shown in the figure, the first step is to prepare the base membrane 110 and the hydrophilic polymer membrane 130.

[0075] Moreover, such as Figure 5 As shown in section (a), the step (S110) is performed to generate a mixture by mixing polyol P and agar powder (not shown in the figure) or pearl powder B and then stirring.

[0076] Moreover, such as Figure 5 As shown in section (a), the step of generating the reactant by adding isocyanate I to the mixture and stirring is performed (S110).

[0077] Moreover, such as Figure 5 As shown in part (b), the steps of pouring the reactant S onto the hydrophilic polymer membrane 130, maintaining its shape to a predetermined thickness and then drying it, forming a porous sheet 120 on the hydrophilic polymer membrane 130, and bonding the hydrophilic polymer membrane 130 to the porous sheet 120 are performed (S120).

[0078] The porous sheet 120 is bonded to the surface of the hydrophilic polymer membrane 130 where convex protrusions 135 are formed.

[0079] Moreover, such as Figure 5 As shown in section (c), the step of performing physical or chemical treatment on the hydrophilic polymer membrane 130 bonded to the porous sheet 120 to form a prescribed pattern or micropores 132 at equal intervals is performed (S130).

[0080] Moreover, such as Figure 5 As shown in section (d), the step of bonding the porous sheet 120 to the base film 110 with the hydrophilic polymer membrane 130 is performed (S140).

[0081] As described above, the present invention has been shown and illustrated through preferred embodiments, but the present invention is not limited to the above embodiments, and various changes and modifications can be made by those skilled in the art without departing from the spirit of the present invention.

Claims

1. A culture membrane mimicking the filamentous structure of *Porphyra yezoensis*, characterized in that, include: The base film layer is composed of biodegradable materials; A filamentous growth layer is formed above the base film layer, creating an environment that allows the filamentous structures of *Porphyra* to penetrate and grow; and A filamentous protective layer is formed on the upper part of the filamentous growth layer, forming multiple micropores that allow the laver filaments to pass through, protecting the laver filaments growing in the filamentous growth layer from external influences.

2. The seashell-like filamentous culture membrane according to claim 1, characterized in that, The filamentous growth layer is composed of a porous sheet made of a mixture or reaction of hydrophilic material and biomaterial that provides nutrients to the laver filaments.

3. The seashell-like filamentous culture membrane according to claim 2, characterized in that, The biological material is one or more of the following powders or liquids: agar, pearl, glucose, white sugar, yeast, chitosan, hyaluronic acid, vitamin A, vitamin E, vitamin K, calcium carbonate, sodium nitrate, calcium phosphate, potassium phosphate, sodium sulfate, calcium hydroxide, and magnesium hydroxide.

4. The seashell-like filamentous culture membrane according to claim 1, characterized in that, The filamentous protective layer is formed by adding an antibacterial and deodorizing agent to a hydrophilic polymer membrane.

5. The seashell-like filamentous culture membrane according to claim 1, characterized in that, The filamentous protective layer has a plurality of convex protrusions arranged longitudinally and laterally on its lower surface facing the filamentous growth layer, and is engaged with the filamentous growth layer through the plurality of convex protrusions.

6. The seashell-like filamentous culture membrane according to claim 1, characterized in that, The base film layer, the filamentous growth layer, and the filamentous protective layer are each composed of one or more of the following: polybutylene adipate terephthalate, polybutylene succinate, polyhydroxyalkanoate, polylactic acid, polycaprolactone, cross-linked polyvinyl alcohol, polyglycolic acid, modified cellulose, and starch-modified resin. The materials of the base film layer, the filamentous growth layer, and the filamentous protective layer may be the same or different from each other.

7. A method for preparing a culture membrane mimicking the filamentous structure of *Porphyra yezoensis*, characterized in that, Includes the following steps: Preparation of base membranes and hydrophilic polymer membranes; The mixture is generated by stirring after mixing polyols and biomaterials; The reactants are generated by adding isocyanate to the mixture and stirring. The reactants are poured onto the hydrophilic polymer membrane, and dried after maintaining their shape to a specified thickness. A porous sheet is then formed on the hydrophilic polymer membrane, and the hydrophilic polymer membrane is bonded to the porous sheet. The hydrophilic polymer membrane is subjected to physical or chemical treatment to form a prescribed pattern or micropores with equal spacing; as well as The porous sheet is bonded to the base film.

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