Composition of bionic sebum film moisturizing factors and preparation method

Abstract

The invention discloses a bionic sebum film moisturizing factor composition and a preparation method, the bionic sebum film moisturizing factor composition is prepared from the following components in parts by weight: 55 to 65 parts of water, 20 to 40 parts of butanediol, 0.05 to 0.2 part of polyglutamic acid, 0.1 to 0.3 part of arginine, 0.05 to 0.2 part of glutamic acid, 0.1 to 0.3 part of valine, 0.05 to 0.2 part of leucine, 0.05 to 0.2 part of isoleucine, 1 to 2 parts of serine, 1 to 2 parts of glycine, 1 to 2 parts of alanine, 0.05 to 0.1 part of proline and 0.1 to 0.3 part of aspartic acid. The preparation method comprises the following steps: preparing all the raw materials in the formula, carrying out pretreatment according to the classification of the raw materials, building a basic solvent dissolving environment, controlling the temperature during solvent dissolving, orderly adding amino acid components in the raw materials in a certain environment and temperature, and uniformly stirring to obtain a finished product. The preparation method comprises the following steps: adjusting the pH value of a solvent during dissolution in stages, accurately controlling the acid-base balance of the solvent, cooling the prepared solvent to be stable, filtering to remove impurities, verifying the final quality of the product, accurately solving the core defects of the existing moisturizing product, and improving the moisturizing efficiency and durability.

Description

Technical Field

[0001] This invention relates to the field of biomimetic sebum film moisturizing factor technology, specifically to a composition and preparation method of a biomimetic sebum film moisturizing factor. Background Technology

[0002] Most existing skin moisturizing products adopt a formula approach similar to natural sebum moisturizing factors, and commonly add amino acids, such as single amino acids or small amounts of compound amino acids, sugars such as glucose, fructose, fatty acids, etc. Through the combination of these ingredients, they play a certain role in skin hydration and help maintain the moisture of the skin surface. A relatively mature application system has been formed in the field of basic moisturizing. While existing moisturizing products achieve some effect in basic hydration, they still have significant shortcomings. They lack the ability to deeply penetrate the skin's surface, and their moisturizing effect is easily weakened by environmental factors such as dryness, low temperatures, and strong winds. They cannot maintain skin hydration for long periods and require frequent reapplication to maintain the moisturizing effect. Furthermore, some formulas have poor compatibility between ingredients due to unreasonable ingredient combinations, which may not only reduce moisturizing efficacy but also cause skin irritation reactions such as redness, itching, and stinging. They are particularly unfriendly to sensitive skin and skin with damaged barriers, making it difficult to balance high efficacy with gentleness, and their stability is poor. At the same time, existing products mostly focus on the basic moisturizing process of hydration and water retention, and do not cover extended functions such as skin barrier repair, anti-aging, and anti-inflammation. They cannot fundamentally improve problems such as dryness, sensitivity, and aging caused by skin barrier damage, and cannot meet consumers' needs for comprehensive skin care, thus limiting their applicability. Summary of the Invention

[0003] The purpose of this invention is to provide a composition and preparation method of a biomimetic sebum film moisturizing factor, in order to solve the problems mentioned in the background art, such as the lack of deep penetration ability of existing moisturizing products into the skin surface, the easy weakening of moisturizing effect due to environmental factors such as dryness, low temperature, and strong wind, the inability to maintain the skin's hydration state for a long time, the need for frequent reapplication to maintain the moisturizing effect, and the poor compatibility between some formulas due to unreasonable ingredient matching, which may not only reduce the moisturizing effect, but also cause skin irritation reactions such as redness, itching, and stinging. In particular, it has low friendliness to special skin types such as sensitive skin and skin with damaged barrier, and it is difficult to achieve a balance between high efficiency and gentleness, and the stability is poor. At the same time, existing products mostly focus on the basic moisturizing link of hydration and water retention, and do not cover the extended functions such as skin barrier repair, anti-aging, and anti-inflammation. They cannot fundamentally improve the dryness, sensitivity, and aging problems caused by skin barrier damage, and cannot meet consumers' needs for comprehensive skin care, and the scope of application is limited.

[0004] Therefore, the present invention provides a composition of a biomimetic sebum film moisturizing factor, characterized in that it comprises the following components by weight: 55-65 parts water, 20-40 parts butylene glycol, 0.05-0.2 parts polyglutamic acid, 0.1-0.3 parts arginine, 0.05-0.2 parts glutamic acid, 0.1-0.3 parts valine, 0.05-0.2 parts leucine, 0.05-0.2 parts isoleucine, 1-2 parts serine, 1-2 parts glycine, 1-2 parts alanine, 0.05-0.1 parts proline, 0.1-0.3 parts aspartic acid, 1-4 parts sodium lactate, 0.05-0.2 parts citric acid (lactic acid), 2-8 parts trehalose, and 0.1-0.3 parts potassium hydroxide.

[0005] Preferably, the specific weight ratio is as follows: 50 parts water, 35 parts butanediol, 0.15 parts polyglutamic acid, 0.3 parts arginine, 0.18 parts glutamic acid, 0.28 parts valine, 0.16 parts leucine, 0.18 parts isoleucine, 1.65 parts serine, 1.65 parts glycine, 1.65 parts alanine, 0.08 parts proline, 0.28 parts aspartic acid, 3 parts sodium lactate, 0.16 parts citric acid, 5 parts trehalose, and 0.28 parts potassium hydroxide.

[0006] Preferably, the raw materials are added in proportion, all components are dissolved in water and stirred evenly. The dissolution temperature is first set to 50°C, and the pH value of the composition is adjusted with citric acid and potassium hydroxide to make the pH value of the composition 5.8. Then the dissolution temperature is set to 70°C, and the pH value of the composition is adjusted with citric acid and potassium hydroxide to make the pH value of the composition 6.4.

[0007] The preferred specific weight ratio is as follows: 50 parts water, 35 parts butanediol, 0.15 parts polyglutamic acid, 0.3 parts arginine, 0.18 parts glutamic acid, 0.28 parts valine, 0.16 parts leucine, 0.18 parts isoleucine, 1.65 parts serine, 1.65 parts glycine, 1.65 parts alanine, 0.08 parts proline, 0.28 parts aspartic acid, 3 parts sodium lactate, 0.16 parts lactic acid, 5 parts trehalose, and 0.28 parts potassium hydroxide.

[0008] Preferably, the raw materials are added in proportion, all components are dissolved in water and stirred evenly. The dissolution temperature is first set to 50°C, and the pH value of the composition is adjusted with lactic acid and potassium hydroxide to make the pH value of the composition 5.8. Then the dissolution temperature is set to 70°C, and the pH value of the composition is adjusted with lactic acid and potassium hydroxide to make the pH value of the composition 6.4.

[0009] Preferably, the method for preparing a biomimetic sebum film moisturizing factor according to claim 1 is characterized by comprising the following steps: S1. Prepare all the raw materials in the formula and pre-treat them according to their classification. S2. Establish a basic solvent dissolution environment and control the temperature during solvent dissolution. Then, under certain environment and temperature, add the amino acid components from the raw materials in an orderly manner. S3. Adjust the pH value during solvent dissolution in stages to precisely control the acid-base balance of the solvent; S4. After the prepared solvent has been cooled and stabilized, perform filtration to remove impurities and verify the final quality of the product.

[0010] Preferably, in step S1, the specific steps for preparing all raw materials in the formula and pre-treating them according to their classification are as follows: (1) Prepare according to the following proportions: 50 parts water, 35 parts butanediol, 0.15 parts polyglutamic acid, 0.3 parts arginine, 0.18 parts glutamic acid, 0.28 parts valine, 0.16 parts leucine, 0.18 parts isoleucine, 1.65 parts serine, 1.65 parts glycine, 1.65 parts alanine, 0.08 parts proline, 0.28 parts aspartic acid, 3 parts sodium lactate, 0.16 parts citric acid, 0.16 parts lactic acid, 5 parts trehalose, and 0.28 parts potassium hydroxide; (2) Divide the raw materials into solvent group, easily soluble efficacy group, amino acid group and pH adjustment group. Put water and butanediol in the solvent group into clean glass beakers and seal them for later use. Mix polyglutamic acid, trehalose and sodium lactate in the easily soluble efficacy group and put them into a dry reagent bottle and place them in a room temperature environment. Divide the ten amino acids in the amino acid group into three levels according to their solubility: high solubility, medium solubility and low solubility. Serine, glycine and alanine in the high solubility group, arginine, glutamic acid and aspartic acid in the medium solubility group, and valine, leucine, isoleucine and proline in the low solubility group into moisture-proof bags for each group. Weigh them quickly and seal them immediately. Put citric acid, lactic acid and potassium hydroxide in the pH adjustment group into light-proof reagent bottles to prevent lactic acid from volatilizing or citric acid from absorbing moisture.

[0011] Preferably, in step S2, the specific steps for establishing a basic solvent dissolution environment, controlling the temperature during solvent dissolution, and then orderly adding the amino acid components from the raw materials under certain environment and temperature are as follows: (1) Set up a solvent dissolution environment. Add the weighed water to the mixing tank, then turn on the stirrer, and slowly pour in a certain proportion of butanediol. Continue stirring for 5-8 minutes until the solution is transparent and without layering. At this time, the solvent system is a water-butanediol mixture, which provides a basic environment for subsequent dissolution. (2) Place the mixing tank into a constant temperature water bath, turn on the heating, and raise the temperature to 50°C at a rate of 2-3°C per minute. After the temperature stabilizes, add polyglutamic acid → trehalose → sodium lactate in sequence. First add polyglutamic acid and stir for 5 minutes until there are no visible particles. Then add trehalose and stir for 3 minutes. Finally add sodium lactate and stir for 2 minutes to ensure that the solution is completely transparent. (3) Add amino acid components in an orderly manner. First, maintain a temperature of 50°C and stir at 300-500 rpm. Add the high-solubility amino acid group first, mix the three together and pour them in at once. Stir for 3 minutes. The solution remains transparent. Then add the medium-solubility amino acid group in two small amounts, with an interval of 3 minutes between each addition. Stir until there is no precipitate. Finally, add the low-solubility amino acid group. Add each component separately and stir for 4 minutes for each. After all the components are added, continue stirring for 5 minutes to confirm that there are no white particles in the solution. After completion, raise the temperature of the constant temperature water bath to 70°C and continue stirring for 10 minutes to consolidate the dissolution effect without damaging the dissolved components. At this time, the solution is a mixture of solvent, active ingredient and amino acid, which provides a uniform system for subsequent pH adjustment.

[0012] Preferably, in step S3, the specific steps for adjusting the pH value during solvent dissolution in stages to precisely control the solvent acid-base balance are as follows: (1) Determine the target pH value and reagents. When using citric acid + potassium hydroxide as the regulator, the target pH value is 5.8. When using lactic acid + potassium hydroxide as the regulator, the target pH value is 6.4, which are both consistent with the natural pH value of the skin. (2) Adjust the pH value in stages. When using citric acid, use a pipette to take 0.05 mL of citric acid each time, add it to the mixture and stir for 1 minute. Measure the pH value with a calibrated pH meter. Repeat the operation until the pH drops to 5.8-5.9. If the pH is lower than 5.8, use a pipette to take 0.02 mL of potassium hydroxide each time, add it and stir for 1 minute. Measure the pH value again until it accurately reaches 5.8, with an error ≤ ±0.05. When using lactic acid, which is weaker than citric acid, take 0.1 mL of lactic acid each time, add it, stir for 2 minutes and measure the pH value until the pH value rises to 6.3-6.4. If the pH value is higher than 6.4, add 0.01 mL of lactic acid each time, stir for 1 minute and measure again until the pH value accurately reaches 6.4, with an error ≤ ±0.05. After adjustment, continue stirring for 10 minutes and measure the pH value again to confirm no fluctuation.

[0013] Preferably, in step S4, after the prepared solvent has been cooled and stabilized, the specific steps for filtering to remove impurities and verifying the final quality of the product are as follows: (1) Cooling and stabilizing operation: Add 25°C room temperature water to the constant temperature water bath to cool the stirring tank. Control the cooling rate to 1-2°C per minute. Keep stirring at 200 rpm during the cooling process. When the solution temperature drops to 25-28°C and is consistent with the room temperature, turn off the stirring, cover the stirring tank, and let it stand in a clean environment for 30 minutes to allow the component molecules to rearrange to a stable state. (2) Filtration and impurity removal operation to remove any possible residual small particles and ensure the purity of the finished product. Under aseptic conditions, the matured mixture is slowly filtered through a 0.22μm cellulose acetate microporous filter membrane into a sterile brown light-proof glass bottle. During the filtration process, the speed is kept uniform to avoid excessive pressure that could cause the filter membrane to rupture. If the filtration speed is too slow, the liquid level can be appropriately reduced to ensure thorough filtration. (3) Verify the final quality of the product and ensure that the product meets the technical requirements. Observe the finished product against a white background. It should be a transparent and uniform liquid without layering, sedimentation, or bubbles. It should meet the appearance requirements for use in skin care products. Insert a calibrated pH meter into the middle of the finished product to measure the pH value and confirm that it is still within the target value range. Finally, take 5 mL of the finished product and store it in a 4℃ refrigerator and a 45℃ oven for 24 hours. After taking it out, observe that there is no layering, discoloration, or odor. Only after all tests are qualified can the finished product be judged as completed.

[0014] The present invention provides a composition and preparation method for a biomimetic sebum film moisturizing factor, the beneficial effects of which are: The composition and preparation method of this moisturizing factor can precisely solve the core defects of existing moisturizing products, improve moisturizing efficacy and longevity. By scientifically compounding a variety of raw materials and amino acids, and using a staged heating and dissolving process, it enhances the deep penetration of the ingredients into the skin surface, effectively resists the effects of environmental factors such as dryness, low temperature, and strong wind on moisturizing effect, can maintain the skin's hydration state for a long time, reduce the need for frequent reapplication, and make up for the problems of insufficient penetration and easy failure of moisturizing in existing products. The composition and preparation method of this moisturizing factor can optimize the compatibility of ingredients, balance high efficiency and gentleness, and expand the range of applicable skin types. The composition uses human secretion products or essential raw materials, and through the process design of adding amino acids according to solubility grade and adjusting the natural pH value of the skin in stages, it avoids the problem of poor compatibility caused by unreasonable ingredient matching. It not only ensures the high efficiency of core effects such as moisturizing and repair, but also significantly reduces the risk of skin irritation such as redness, itching, and stinging. It is highly friendly to special skin types such as sensitive skin and skin with damaged barrier, breaking the limitation of existing products that are difficult to balance high efficiency and gentleness. The composition and preparation method of this moisturizing factor can enrich the functional dimensions and achieve integrated moisturizing, repair and maintenance. Compared with existing single-function products that focus on hydration and water retention, the composition of this invention contains amino acids such as arginine, leucine and isoleucine, which can promote skin repair and maintain barrier integrity; proline can enhance skin elasticity; and trehalose has both antioxidant and anti-inflammatory auxiliary effects. On the basis of highly efficient moisturizing, it simultaneously covers skin barrier repair, anti-aging and anti-inflammatory functions, which can fundamentally improve the dryness, sensitivity and aging problems of the skin caused by barrier damage, meet consumers' needs for comprehensive skin care, and enhance product competitiveness. Attached Figure Description

[0015] Figure 1 This is a compositional diagram of a biomimetic sebum film moisturizing factor composition of the present invention; Figure 2 This is a flowchart of a method for preparing a biomimetic sebum film moisturizing factor according to the present invention. Detailed Implementation

[0016] The technical solution of the present invention will now be described in detail through specific embodiments.

[0017] Example 1: Please see Figure 1 This invention provides a composition of a biomimetic sebum film moisturizing factor, characterized by comprising the following components by weight: 55-65 parts water, 20-40 parts butylene glycol, 0.05-0.2 parts polyglutamic acid, 0.1-0.3 parts arginine, 0.05-0.2 parts glutamic acid, 0.1-0.3 parts valine, 0.05-0.2 parts leucine, 0.05-0.2 parts isoleucine, 1-2 parts serine, 1-2 parts glycine, 1-2 parts alanine, 0.05-0.1 parts proline, 0.1-0.3 parts aspartic acid, 1-4 parts sodium lactate, 0.05-0.2 parts citric acid (lactic acid), 2-8 parts trehalose, and 0.1-0.3 parts potassium hydroxide.

[0018] Furthermore, the specific weight proportions are as follows: 50 parts water, 35 parts butanediol, 0.15 parts polyglutamic acid, 0.3 parts arginine, 0.18 parts glutamic acid, 0.28 parts valine, 0.16 parts leucine, 0.18 parts isoleucine, 1.65 parts serine, 1.65 parts glycine, 1.65 parts alanine, 0.08 parts proline, 0.28 parts aspartic acid, 3 parts sodium lactate, 0.16 parts citric acid, 5 parts trehalose, and 0.28 parts potassium hydroxide. In this embodiment, water serves as a solvent, providing a basic carrying environment for all components. Butylene glycol acts as a humectant and solvent, aiding in moisture absorption and improving skin hydration. Together, they form the basic system. Polyglutamic acid has a strong water-absorbing capacity, helping the skin retain moisture. Trehalose, as a natural sugar, helps maintain skin hydration and has antioxidant effects. Sodium lactate helps the skin retain moisture and has a mild exfoliating effect. These three components enhance moisturizing and antioxidant capabilities while also aiding in the absorption of subsequent ingredients. Each of the 10 amino acids has its own function: arginine helps maintain skin moisture and promotes skin repair; leucine helps maintain the integrity of the skin barrier; and proline participates in skin repair and enhances skin elasticity. Glutamic acid has moisturizing and skin-soothing effects, valine has antioxidant and nourishing effects, isoleucine helps repair the skin barrier and promotes skin health, serine has moisturizing and soothing effects, glycine can promote skin hydration and maintain skin softness, alanine helps moisturize the skin, and aspartic acid has moisturizing and skin-soothing effects. The precise composition ratio of the composition was clearly defined, and reasonable adjustments were made within the basic ratio range. By fixing the precise dosage of each component, a foundation was provided for the standardized operation and efficacy stability of the subsequent preparation process. At the same time, the selected components were all combined around the core objectives of moisturizing and repairing the skin barrier, which is in line with the technical concept of simulating the skin's natural sebum film.

[0019] Furthermore, add the raw materials according to the proportion, dissolve all components in water, stir evenly, first set the dissolution temperature to 50℃, and adjust the pH value of the composition with citric acid and potassium hydroxide to make the pH value of the composition 5.8, then set the dissolution temperature to 70℃, and adjust the pH value of the composition with citric acid and potassium hydroxide to make the pH value of the composition 6.4. In this embodiment, the low temperature of 50°C ensures that the activity of heat-sensitive components such as polyglutamic acid, arginine, and serine is not destroyed, while allowing citric acid and potassium hydroxide to play a pH-regulating role, bringing the system pH value to 5.8 first. The temperature is then raised to 70°C, which on the one hand consolidates the solubility of butylene glycol in each component, ensuring that components such as sodium lactate and trehalose are evenly dispersed and avoiding weakened effects due to uneven dissolution. On the other hand, by adjusting the pH value to 6.4 a second time, the acidity and alkalinity of the system are further optimized, matching the skin's natural pH environment to reduce irritation, and allowing all components to function stably at a suitable pH value.

[0020] A method for preparing a biomimetic sebum film moisturizing factor, characterized by comprising the following steps: S1. Prepare all raw materials in the formula and pre-treat them according to their classification. The specific steps for preparing all raw materials in the formula and pre-treating them according to their classification are as follows: Prepare the following ingredients according to the proportions: 50 parts water, 35 parts butanediol, 0.15 parts polyglutamic acid, 0.3 parts arginine, 0.18 parts glutamic acid, 0.28 parts valine, 0.16 parts leucine, 0.18 parts isoleucine, 1.65 parts serine, 1.65 parts glycine, 1.65 parts alanine, 0.08 parts proline, 0.28 parts aspartic acid, 3 parts sodium lactate, 0.16 parts citric acid, 5 parts trehalose, and 0.28 parts potassium hydroxide. The raw materials were divided into solvent group, easily soluble functional group, amino acid group, and pH adjustment group. Water and butanediol from the solvent group were separately placed in clean glass beakers and sealed for later use. Polyglutamic acid, trehalose, and sodium lactate from the easily soluble functional group were mixed and placed in a desiccating reagent bottle and stored at room temperature. The ten amino acids in the amino acid group were further divided into three levels according to solubility: high solubility, medium solubility, and low solubility. Serine, glycine, and alanine (high solubility), arginine, glutamic acid, and aspartic acid (medium solubility), and valine, leucine, isoleucine, and proline (low solubility) were each individually packaged in a moisture-proof bag, quickly weighed, and immediately sealed. Citric acid and potassium hydroxide from the pH adjustment group were separately placed in light-proof reagent bottles to prevent citric acid from absorbing moisture. In this embodiment, water and butylene glycol are classified as solvents and sealed separately to prevent impurities from contaminating the basic carrying capacity of water and the hygroscopic and skin-hydrating effects of butylene glycol. Polyglutamic acid, trehalose, and sodium lactate are classified as easily soluble functional groups, and room temperature storage ensures the stability of their core functions such as moisturizing and anti-oxidation. This prevents the water absorption capacity of polyglutamic acid from decreasing and the antioxidant effect of trehalose from weakening due to improper storage. The 10 amino acids are packaged according to their solubility, which not only prevents the amino acids from absorbing moisture and deteriorating, but also prevents glycine, serine, and other components from weakening their hydration and soothing effects due to moisture absorption. This also lays the foundation for the orderly addition of each amino acid and the full utilization of its functions. Citric acid and potassium hydroxide are classified as pH adjustment groups and stored away from light to prevent citric acid from absorbing moisture and affecting the accuracy of pH adjustment, and potassium hydroxide from deteriorating and causing pH adjustment failure. This ensures that the pH value can be accurately adjusted afterward, ensuring product stability and the normal functioning of each component. The overall pretreatment logic is completely consistent with the functional and characteristic requirements of each component in the document.

[0021] S2. Establish a basic solvent dissolution environment and control the temperature during solvent dissolution. Then, under specific environmental conditions and temperature, add the amino acid components from the raw materials in an orderly manner. The specific steps for establishing a basic solvent dissolution environment and controlling the temperature during solvent dissolution, and then adding the amino acid components from the raw materials in an orderly manner under specific environmental conditions and temperature, are as follows: To create a solvent dissolution environment, add the weighed water to the mixing tank, then turn on the stirrer, and slowly pour in a certain proportion of butanediol. Continue stirring for 5-8 minutes until the solution is transparent and without layering. At this point, the solvent system is a water-butanediol mixture, which provides the basic environment for subsequent dissolution. Place the mixing jar in a constant temperature water bath, turn on the heating, and raise the temperature to 50°C at a rate of 2-3°C per minute. After the temperature stabilizes, add polyglutamic acid, trehalose, and sodium lactate in sequence. First add polyglutamic acid and stir for 5 minutes until there are no visible particles. Then add trehalose and stir for 3 minutes. Finally, add sodium lactate and stir for 2 minutes to ensure that the solution is completely transparent. Add the amino acid components in an orderly manner. First, maintain the temperature at 50℃ and stir at 300-500 rpm. Add the high-soluble amino acid group first, mix the three together and pour in all at once. Stir for 3 minutes until the solution remains transparent. Then add the medium-soluble amino acid group in two small amounts, 3 minutes apart each time, and stir until there is no precipitate. Finally, add the low-soluble amino acid group, adding each component separately and stirring for 4 minutes each. After all components are added, continue stirring for 5 minutes until the solution is free of white particles. After completion, raise the temperature of the constant temperature water bath to 70℃ and stir continuously for 10 minutes to consolidate the dissolution effect without damaging the dissolved components. At this point, the solution is a mixture of solvent, active ingredient, and amino acid, providing a homogeneous system for subsequent pH adjustment. In this embodiment, a transparent water-butylene glycol mixture was constructed. Utilizing the basic solvent-carrying capacity of water and the dual moisturizing and solvent properties of butylene glycol, a favorable environment was provided for the dissolution of subsequent components, ensuring uniform dispersion of moisturizing and repairing ingredients. Polyglutamic acid, trehalose, and sodium lactate were dissolved at 50°C. This temperature ensures complete dissolution of all three, preventing insufficient dissolution of polyglutamic acid from reducing its water absorption capacity and uneven dissolution of trehalose from affecting its antioxidant effect. High temperatures also prevent the degradation of their activity. Subsequently, amino acids were added in the order of high solubility → medium solubility → low solubility: first, readily soluble amino acids were added to ensure rapid dissolution and basic moisturizing effects; then, medium-soluble amino acids were added in two stages to avoid uneven repair and soothing effects due to excessively high local concentrations; finally, low-soluble amino acids were added separately with extended stirring time to ensure complete dissolution and to exert their antioxidant, barrier maintenance, and repair effects. After completion, the temperature was raised to 70°C to consolidate the dissolution effect, allowing all components to form a stable system. This ensures that the hygroscopic effects of butylene glycol, the water-locking effects of polyglutamic acid, and the repairing effects of amino acids work synergistically, without any loss of efficacy due to component precipitation.

[0022] S3. Adjust the pH value during solvent dissolution in stages to precisely control the solvent's acid-base balance; the specific steps for adjusting the pH value during solvent dissolution in stages to precisely control the solvent's acid-base balance are as follows: Determine the target pH level and reagents. When using citric acid and potassium hydroxide as regulators, the target pH level is 5.8, which matches the skin's natural pH level. Adjust the pH value in stages. When using citric acid, pipette 0.05 mL of citric acid each time, add it to the mixture, stir for 1 minute, and measure the pH value with a calibrated pH meter. Repeat the operation until the pH drops to 5.8-5.9. If the pH is lower than 5.8, pipette 0.02 mL of potassium hydroxide each time, add it to the mixture, stir for 1 minute, and remeasure the pH value until it accurately reaches 5.8, with an error ≤ ±0.05. In this embodiment, citric acid can adjust the product's pH and has a slight exfoliating effect. Potassium hydroxide is commonly used to adjust pH to ensure the product's stability and effectiveness. The target pH is set at 5.8. Citric acid's pH-regulating effect makes the system's pH suitable, reducing skin irritation and preventing the repairing effect of arginine and the soothing effect of serine from being affected by excessively high or low pH. Citric acid's slight exfoliating effect also assists in the powerful moisturizing effect of polyglutamic acid, the antioxidant effect of trehalose, and the penetration of various amino acid repair components, enhancing overall efficacy. Through phased operation, the pH error is controlled within ±0.05, avoiding sudden pH changes due to excessive addition of regulators at once. This prevents polyglutamic acid from precipitating out due to improper pH and losing its water-absorbing capacity, and prevents amino acids from losing activity due to pH imbalance, thus weakening their repair and moisturizing effects. This ensures that all ingredients precisely perform their functions in a stable pH environment, such as isoleucine repairing the skin barrier, trehalose providing antioxidant effects, and sodium lactate moisturizing, guaranteeing the overall efficacy and gentleness of the product.

[0023] S4. After the prepared solvent has cooled and stabilized, perform filtration to remove impurities and verify the final product quality. The specific steps for performing filtration to remove impurities and verifying the final product quality after the prepared solvent has cooled and stabilized are as follows: For cooling and stabilization, add 25°C room temperature water to the constant temperature water bath to cool the stirring tank. Control the cooling rate to 1-2°C per minute and keep stirring at 200 rpm during the cooling process. When the solution temperature drops to 25-28°C, which is consistent with room temperature, turn off the stirring, cover the stirring tank, and let it stand in a clean environment for 30 minutes to allow the component molecules to rearrange to a stable state. The filtration process removes any remaining tiny particles to ensure the purity of the finished product. Under aseptic conditions, the matured mixture is slowly filtered through a 0.22μm cellulose acetate microporous membrane into a sterile brown light-proof glass bottle. The filtration process is carried out at a constant speed to avoid excessive pressure that could cause the membrane to rupture. If the filtration speed is too slow, the liquid level can be appropriately reduced to ensure thorough filtration. To verify the final quality of the product and ensure that it meets the technical requirements, observe the finished product against a white background. It should be a transparent, uniform liquid without layering, sedimentation, or bubbles, meeting the appearance requirements for use in skincare products. Insert a calibrated pH meter into the middle of the finished product to measure the pH value and confirm that it is still within the target range. Finally, take 5 mL of the finished product and store it in a 4℃ refrigerator and a 45℃ oven for 24 hours respectively. After taking it out, observe that there is no layering, discoloration, or odor. Only after all tests are qualified can the finished product be judged as completed. In this embodiment, the product is slowly cooled to room temperature and allowed to mature, allowing the molecules of water, butylene glycol, polyglutamic acid, and other components to arrange themselves stably. This prevents the components from separating due to rapid cooling and ensures that the water-locking effect of polyglutamic acid and the hygroscopic effect of butylene glycol can continue to function. A 0.22μm filter membrane is used to remove impurities and any remaining microparticles. This prevents particles from affecting the product's usability and also prevents impurities from interfering with the skin-repairing effect of arginine, the soothing effect of glutamic acid, and the antioxidant effect of trehalose, ensuring that the efficacy of each component is precisely applied to the skin. Visual inspection confirms that all components, including polyglutamic acid and amino acids, are completely dissolved without precipitation, ensuring the uniformity of each component's effect. pH testing is repeated to ensure that the pH-regulating effect of citric acid and potassium hydroxide remains effective, providing a stable environment for the components to function. High and low temperature stability tests verify that polyglutamic acid retains its water-absorbing capacity at low temperatures and does not degrade at high temperatures, that amino acids retain their repair and moisturizing effects at extreme temperatures, and that the antioxidant effect of trehalose is not affected by temperature. The final product is a biomimetic sebum film moisturizing factor composition that meets functional requirements, effectively guaranteeing the function of each component described in the document.

[0024] Example 2: Please see Figure 1 This invention provides a composition of a biomimetic sebum film moisturizing factor, characterized by comprising the following components by weight: 55-65 parts water, 20-40 parts butylene glycol, 0.05-0.2 parts polyglutamic acid, 0.1-0.3 parts arginine, 0.05-0.2 parts glutamic acid, 0.1-0.3 parts valine, 0.05-0.2 parts leucine, 0.05-0.2 parts isoleucine, 1-2 parts serine, 1-2 parts glycine, 1-2 parts alanine, 0.05-0.1 parts proline, 0.1-0.3 parts aspartic acid, 1-4 parts sodium lactate, 0.05-0.2 parts citric acid (lactic acid), 2-8 parts trehalose, and 0.1-0.3 parts potassium hydroxide.

[0025] Furthermore, the specific weight proportions are as follows: 50 parts water, 35 parts butanediol, 0.15 parts polyglutamic acid, 0.3 parts arginine, 0.18 parts glutamic acid, 0.28 parts valine, 0.16 parts leucine, 0.18 parts isoleucine, 1.65 parts serine, 1.65 parts glycine, 1.65 parts alanine, 0.08 parts proline, 0.28 parts aspartic acid, 3 parts sodium lactate, 0.16 parts lactic acid, 5 parts trehalose, and 0.28 parts potassium hydroxide. In this embodiment, by determining the precise weight ratio of 17 ingredients, including 50 parts water and 35 parts butylene glycol, the synergistic adaptation of the functions of each ingredient is achieved. Water, as a solvent, provides a basic carrying environment for all ingredients. Butylene glycol plays a dual role as a moisturizer and solvent to improve skin hydration. Polyglutamic acid helps the skin retain moisture with its strong water-absorbing capacity. Trehalose, as a natural sugar, maintains skin hydration and has antioxidant effects. Sodium lactate assists in skin moisturizing and has a mild exfoliating effect. Ten amino acids each play their role: arginine helps maintain skin moisture and promotes repair; leucine ensures the integrity of the skin barrier; isoleucine assists in repairing the skin barrier; serine, glycine, alanine, glutamic acid, and aspartic acid all provide moisturizing and soothing effects; valine plays an antioxidant and nourishing role; proline participates in skin repair and enhances elasticity; and lactic acid and potassium hydroxide are used together for subsequent pH adjustment. The precise dosage and total compatibility of all ingredients ensure that the function of each ingredient is fully utilized, forming a moisturizing and repairing system that fits the concept of a biomimetic sebum film.

[0026] Furthermore, add the raw materials according to the proportion, dissolve all components in water, stir evenly, first set the dissolution temperature to 50℃, and adjust the pH value of the composition with lactic acid and potassium hydroxide to make the pH value of the composition 5.8, then set the dissolution temperature to 70℃, and adjust the pH value of the composition with lactic acid and potassium hydroxide to make the pH value of the composition 6.4. In this embodiment, the low temperature of 50°C can prevent heat-sensitive components such as polyglutamic acid and amino acids from being deactivated by high temperature, and allow lactic acid and potassium hydroxide to initially adjust the pH to 5.8, providing a basic acid-base environment for component dissolution and functional performance. After raising the temperature to 70°C, on the one hand, the solvent effect of butylene glycol can be enhanced to ensure that components such as polyglutamic acid, trehalose, and sodium lactate are completely and evenly dispersed, avoiding the reduction of moisturizing and antioxidant effects due to uneven dissolution. On the other hand, by adjusting the pH to 6.4 a second time, the acidity and alkalinity of the system can be further optimized, which can reduce skin irritation and allow each component to function stably in a suitable environment. At the same time, the mild exfoliating effect of lactic acid can also better assist the penetration of other moisturizing and repairing ingredients.

[0027] A method for preparing a biomimetic sebum film moisturizing factor, characterized by comprising the following steps: S1. Prepare all raw materials in the formula and pre-treat them according to their classification. The specific steps for preparing all raw materials in the formula and pre-treating them according to their classification are as follows: Prepare the following ingredients according to the proportions: 50 parts water, 35 parts butanediol, 0.15 parts polyglutamic acid, 0.3 parts arginine, 0.18 parts glutamic acid, 0.28 parts valine, 0.16 parts leucine, 0.18 parts isoleucine, 1.65 parts serine, 1.65 parts glycine, 1.65 parts alanine, 0.08 parts proline, 0.28 parts aspartic acid, 3 parts sodium lactate, 0.16 parts lactic acid, 5 parts trehalose, and 0.28 parts potassium hydroxide. The raw materials were divided into solvent group, easily soluble functional group, amino acid group, and pH adjustment group. Water and butanediol from the solvent group were separately placed in clean glass beakers and sealed for later use. Polyglutamic acid, trehalose, and sodium lactate from the easily soluble functional group were mixed and placed in a desiccated reagent bottle and stored at room temperature. The ten amino acids in the amino acid group were further divided into three levels according to solubility: high solubility, medium solubility, and low solubility. Serine, glycine, and alanine (high solubility), arginine, glutamic acid, and aspartic acid (medium solubility), and valine, leucine, isoleucine, and proline (low solubility) were each individually packaged in a moisture-proof bag, quickly weighed, and immediately sealed. Lactic acid and potassium hydroxide from the pH adjustment group were separately placed in light-proof reagent bottles to prevent lactic acid evaporation. In this embodiment, water and butylene glycol in the solvent group are sealed separately to prevent impurities from affecting their basic functions. Polyglutamic acid, trehalose, and sodium lactate in the easily soluble efficacy group are stored at room temperature to prevent improper storage from causing a decline in core functions such as water absorption and anti-oxidation. The amino acid group is packaged according to solubility, which not only prevents hygroscopic amino acids such as serine and glycine from losing their moisturizing and soothing effects due to moisture absorption, but also lays the foundation for subsequent orderly dissolution and uniform exertion of repair and moisturizing functions. Lactic acid and potassium hydroxide in the pH adjustment group are stored in the dark to prevent lactic acid volatilization from affecting the pH adjustment accuracy, ensuring that the two can accurately control the acidity and alkalinity of the system and ensure that all components function normally. The overall operation conforms to the functional characteristics of each component.

[0028] S2. Establish a basic solvent dissolution environment and control the temperature during solvent dissolution. Then, under specific environmental conditions and temperature, add the amino acid components from the raw materials in an orderly manner. The specific steps for establishing a basic solvent dissolution environment and controlling the temperature during solvent dissolution, and then adding the amino acid components from the raw materials in an orderly manner under specific environmental conditions and temperature, are as follows: To create a solvent dissolution environment, add the weighed water to the mixing tank, then turn on the stirrer, and slowly pour in a certain proportion of butanediol. Continue stirring for 5-8 minutes until the solution is transparent and without layering. At this point, the solvent system is a water-butanediol mixture, which provides the basic environment for subsequent dissolution. Place the mixing jar in a constant temperature water bath, turn on the heating, and raise the temperature to 50°C at a rate of 2-3°C per minute. After the temperature stabilizes, add polyglutamic acid, trehalose, and sodium lactate in sequence. First add polyglutamic acid and stir for 5 minutes until there are no visible particles. Then add trehalose and stir for 3 minutes. Finally, add sodium lactate and stir for 2 minutes to ensure that the solution is completely transparent. Add the amino acid components in an orderly manner. First, maintain the temperature at 50℃ and stir at 300-500 rpm. Add the high-soluble amino acid group first, mix the three together and pour in all at once. Stir for 3 minutes until the solution remains transparent. Then add the medium-soluble amino acid group in two small amounts, 3 minutes apart each time, and stir until there is no precipitate. Finally, add the low-soluble amino acid group, adding each component separately and stirring for 4 minutes each. After all components are added, continue stirring for 5 minutes until the solution is free of white particles. After completion, raise the temperature of the constant temperature water bath to 70℃ and stir continuously for 10 minutes to consolidate the dissolution effect without damaging the dissolved components. At this point, the solution is a mixture of solvent, active ingredient, and amino acid, providing a homogeneous system for subsequent pH adjustment. In this embodiment, a transparent water-butanediol mixture was constructed. Water's carrying capacity and butanediol's moisturizing and solvent properties provided a favorable environment for the subsequent dissolution of components. Polyglutamic acid, trehalose, and sodium lactate were dissolved at 50°C. This temperature allowed polyglutamic acid to fully dissolve and exert its strong water-absorbing capacity, and trehalose to disperse evenly and release its antioxidant effect, while avoiding high temperatures that could damage its activity. Subsequently, amino acids were added in the following order: high solubility → medium solubility → low solubility. High solubility provided rapid basic moisturizing effects; medium solubility was added in two stages to avoid uneven repair and soothing effects due to excessively high local concentrations; and low solubility was added alone with prolonged stirring to ensure full dissolution and to exert its antioxidant, barrier maintenance, and repair effects. Finally, the temperature was raised to 70°C to consolidate the dissolution effect, forming a stable mixture system. This ensured that the hygroscopic functions of butanediol, the water-locking functions of polyglutamic acid, and the repair functions of amino acids worked synergistically, without any functional loss due to component precipitation.

[0029] S3. Adjust the pH value during solvent dissolution in stages to precisely control the solvent's acid-base balance; the specific steps for adjusting the pH value during solvent dissolution in stages to precisely control the solvent's acid-base balance are as follows: Determine the target pH value and reagents. When using lactic acid + potassium hydroxide as regulators, the target pH value is 6.4, which matches the skin's natural pH value. Adjust the pH value in stages. When using lactic acid for adjustment, lactic acid is weaker than citric acid. Each time, add 0.1 mL of lactic acid dropwise, stir for 2 minutes, and then measure the pH value until the pH value rises to 6.3-6.4. If the pH value is higher than 6.4, add 0.01 mL of lactic acid each time, stir for 1 minute, and then measure again until the pH value accurately reaches 6.4, with an error ≤ ±0.05. After adjustment, continue stirring for 10 minutes and measure the pH value again to confirm that there is no fluctuation. In this embodiment, lactic acid and potassium hydroxide were used as regulators, with a target pH of 6.4. This reduces skin irritation and allows the components to function in a suitable acid-base environment. Considering that lactic acid is less acidic than citric acid, a method of adding small amounts of lactic acid multiple times with real-time monitoring was adopted. Each time, 0.1 mL of lactic acid was added, stirred, and retested. When the pH approached the target value, 0.01 mL was added for fine-tuning, keeping the pH error within ±0.05. This prevented polyglutamic acid from precipitating and losing its water-absorbing capacity, and amino acid activity from being damaged and losing its repair and moisturizing effects due to sudden pH changes. After adjustment, stirring and retesting were continued to ensure pH stability, thus ensuring the stable performance of polyglutamic acid's water-locking, isoleucine's barrier-repairing, and trehalose's antioxidant functions.

[0030] S4. After the prepared solvent has cooled and stabilized, perform filtration to remove impurities and verify the final product quality. The specific steps for performing filtration to remove impurities and verifying the final product quality after the prepared solvent has cooled and stabilized are as follows: For cooling and stabilization, add 25°C room temperature water to the constant temperature water bath to cool the stirring tank. Control the cooling rate to 1-2°C per minute and keep stirring at 200 rpm during the cooling process. When the solution temperature drops to 25-28°C, which is consistent with room temperature, turn off the stirring, cover the stirring tank, and let it stand in a clean environment for 30 minutes to allow the component molecules to rearrange to a stable state. The filtration process removes any remaining tiny particles to ensure the purity of the finished product. Under aseptic conditions, the matured mixture is slowly filtered through a 0.22μm cellulose acetate microporous membrane into a sterile brown light-proof glass bottle. The filtration process is carried out at a constant speed to avoid excessive pressure that could cause the membrane to rupture. If the filtration speed is too slow, the liquid level can be appropriately reduced to ensure thorough filtration. To verify the final quality of the product and ensure that it meets the technical requirements, observe the finished product against a white background. It should be a transparent, uniform liquid without layering, sedimentation, or bubbles, meeting the appearance requirements for use in skincare products. Insert a calibrated pH meter into the middle of the finished product to measure the pH value and confirm that it is still within the target range. Finally, take 5 mL of the finished product and store it in a 4℃ refrigerator and a 45℃ oven for 24 hours respectively. After taking it out, observe that there is no layering, discoloration, or odor. Only after all tests are qualified can the finished product be judged as completed. In this embodiment, the product is slowly cooled to room temperature and allowed to mature, allowing the molecules of water, butylene glycol, polyglutamic acid, and other components to arrange themselves stably. This ensures that the water-locking function of polyglutamic acid and the moisture-absorbing function of butylene glycol continue to function. A 0.22μm filter membrane removes tiny particles, preventing impurities from interfering with the precise functioning of arginine repair, glutamic acid soothing, and trehalose antioxidant properties. In the quality verification process, visual inspection ensures that the components are completely dissolved and free of precipitation, guaranteeing the uniformity of each component's effect. pH retesting ensures the stability of the acid-base environment and maintains the function of the components. High and low temperature stability testing verifies that polyglutamic acid retains its water-absorbing capacity at extreme temperatures, amino acids still have repair and moisturizing effects, and the antioxidant effect of trehalose is unaffected. Ultimately, this ensures that the finished product meets the functional requirements, effectively guaranteeing the moisturizing, repairing, and antioxidant effects of each component.

[0031] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A composition of a biomimetic sebum film moisturizing factor, characterized in that, It includes the following components by weight: 55-65 parts water, 20-40 parts butylene glycol, 0.05-0.2 parts polyglutamic acid, 0.1-0.3 parts arginine, 0.05-0.2 parts glutamic acid, 0.1-0.3 parts valine, 0.05-0.2 parts leucine, 0.05-0.2 parts isoleucine, 1-2 parts serine, 1-2 parts glycine, 1-2 parts alanine, 0.05-0.1 parts proline, 0.1-0.3 parts aspartic acid, 1-4 parts sodium lactate, 0.05-0.2 parts citric acid (lactic acid), 2-8 parts trehalose, and 0.1-0.3 parts potassium hydroxide.

2. The composition of a biomimetic sebum film moisturizing factor according to claim 1, characterized in that: The specific weight proportions are as follows: 50 parts water, 35 parts butanediol, 0.15 parts polyglutamic acid, 0.3 parts arginine, 0.18 parts glutamic acid, 0.28 parts valine, 0.16 parts leucine, 0.18 parts isoleucine, 1.65 parts serine, 1.65 parts glycine, 1.65 parts alanine, 0.08 parts proline, 0.28 parts aspartic acid, 3 parts sodium lactate, 0.16 parts citric acid, 5 parts trehalose, and 0.28 parts potassium hydroxide.

3. The composition of a biomimetic sebum film moisturizing factor according to claim 2, characterized in that: Add the raw materials according to the proportion, dissolve all components in water, stir evenly, first set the dissolution temperature to 50℃, and adjust the pH value of the composition with citric acid and potassium hydroxide to make the pH value of the composition 5.8, then set the dissolution temperature to 70℃, and adjust the pH value of the composition with citric acid and potassium hydroxide to make the pH value of the composition 6.

4.

4. The composition of a biomimetic sebum film moisturizing factor according to claim 1, characterized in that: The specific weight proportions are as follows: 50 parts water, 35 parts butanediol, 0.15 parts polyglutamic acid, 0.3 parts arginine, 0.18 parts glutamic acid, 0.28 parts valine, 0.16 parts leucine, 0.18 parts isoleucine, 1.65 parts serine, 1.65 parts glycine, 1.65 parts alanine, 0.08 parts proline, 0.28 parts aspartic acid, 3 parts sodium lactate, 0.16 parts lactic acid, 5 parts trehalose, and 0.28 parts potassium hydroxide.

5. The composition of a biomimetic sebum film moisturizing factor according to claim 4, characterized in that: Add the raw materials according to the proportion, dissolve all components in water, stir evenly, first set the dissolution temperature to 50℃, and adjust the pH value of the composition with lactic acid and potassium hydroxide to make the pH value of the composition 5.

8. Then set the dissolution temperature to 70℃, and adjust the pH value of the composition with lactic acid and potassium hydroxide to make the pH value of the composition 6.

4.

6. The method for preparing a biomimetic sebum film moisturizing factor according to claim 1, characterized in that: Includes the following steps: S1. Prepare all the raw materials in the formula and pre-treat them according to their classification. S2. Establish a basic solvent dissolution environment and control the temperature during solvent dissolution. Then, under certain environment and temperature, add the amino acid components from the raw materials in an orderly manner. S3. Adjust the pH value during solvent dissolution in stages to precisely control the acid-base balance of the solvent; S4. After the prepared solvent has been cooled and stabilized, perform filtration to remove impurities and verify the final quality of the product.

7. The method for preparing a biomimetic sebum film moisturizing factor according to claim 6, characterized in that: In step S1, the specific steps for preparing all raw materials in the formula and pre-treating them according to their classification are as follows: (1) Prepare according to the following proportions: 50 parts water, 35 parts butanediol, 0.15 parts polyglutamic acid, 0.3 parts arginine, 0.18 parts glutamic acid, 0.28 parts valine, 0.16 parts leucine, 0.18 parts isoleucine, 1.65 parts serine, 1.65 parts glycine, 1.65 parts alanine, 0.08 parts proline, 0.28 parts aspartic acid, 3 parts sodium lactate, 0.16 parts citric acid, 0.16 parts lactic acid, 5 parts trehalose, and 0.28 parts potassium hydroxide; (2) Divide the raw materials into solvent group, easily soluble efficacy group, amino acid group and pH adjustment group. Put water and butanediol in the solvent group into clean glass beakers and seal them for later use. Mix polyglutamic acid, trehalose and sodium lactate in the easily soluble efficacy group and put them into a dry reagent bottle and place them in a room temperature environment. Divide the ten amino acids in the amino acid group into three levels according to their solubility: high solubility, medium solubility and low solubility. Serine, glycine and alanine in the high solubility group, arginine, glutamic acid and aspartic acid in the medium solubility group, and valine, leucine, isoleucine and proline in the low solubility group into moisture-proof bags for each group. Weigh them quickly and seal them immediately. Put citric acid, lactic acid and potassium hydroxide in the pH adjustment group into light-proof reagent bottles to prevent lactic acid from volatilizing or citric acid from absorbing moisture.

8. The method for preparing a biomimetic sebum film moisturizing factor according to claim 6, characterized in that: In step S2, the specific steps for establishing a basic solvent dissolution environment, controlling the temperature during solvent dissolution, and then orderly adding the amino acid components from the raw materials under certain environment and temperature are as follows: (1) Set up a solvent dissolution environment. Add the weighed water to the mixing tank, then turn on the stirrer, and slowly pour in a certain proportion of butanediol. Continue stirring for 5-8 minutes until the solution is transparent and without layering. At this time, the solvent system is a water-butanediol mixture, which provides a basic environment for subsequent dissolution. (2) Place the mixing tank into a constant temperature water bath, turn on the heating, and raise the temperature to 50°C at a rate of 2-3°C per minute. After the temperature stabilizes, add polyglutamic acid, trehalose, and sodium lactate in sequence. First add polyglutamic acid and stir for 5 minutes until there are no visible particles. Then add trehalose and stir for 3 minutes. Finally add sodium lactate and stir for 2 minutes to ensure that the solution is completely transparent. (3) Add amino acid components in an orderly manner. First, maintain a temperature of 50°C and stir at 300-500 rpm. Add the high-solubility amino acid group first, mix the three together and pour them in at once. Stir for 3 minutes. The solution remains transparent. Then add the medium-solubility amino acid group in two small amounts, with an interval of 3 minutes between each addition. Stir until there is no precipitate. Finally, add the low-solubility amino acid group. Add each component separately and stir for 4 minutes for each. After all the components are added, continue stirring for 5 minutes to confirm that there are no white particles in the solution. After completion, raise the temperature of the constant temperature water bath to 70°C and continue stirring for 10 minutes to consolidate the dissolution effect without damaging the dissolved components. At this time, the solution is a mixture of solvent, active ingredient and amino acid, which provides a uniform system for subsequent pH adjustment.

9. The method for preparing a biomimetic sebum film moisturizing factor according to claim 6, characterized in that: In step S3, the specific steps for precisely controlling the solvent acid-base balance by adjusting the pH value during solvent dissolution in stages are as follows: (1) Determine the target pH value and reagents. When using citric acid and potassium hydroxide as regulators, the target pH value is 5.

8. When using lactic acid and potassium hydroxide as regulators, the target pH value is 6.

4. (2) Adjust the pH value in stages. When using citric acid, use a pipette to take 0.05 mL of citric acid each time, add it to the mixture and stir for 1 minute. Measure the pH value with a calibrated pH meter. Repeat the operation until the pH drops to 5.8-5.

9. If the pH is lower than 5.8, use a pipette to take 0.02 mL of potassium hydroxide each time, add it and stir for 1 minute. Measure the pH value again until it accurately reaches 5.8, with an error ≤ ±0.

05. When using lactic acid, which is weaker than citric acid, take 0.1 mL of lactic acid each time, add it, stir for 2 minutes and measure the pH value until the pH value rises to 6.3-6.

4. If the pH value is higher than 6.4, add 0.01 mL of lactic acid each time, stir for 1 minute and measure again until the pH value accurately reaches 6.4, with an error ≤ ±0.

05. After adjustment, continue stirring for 10 minutes and measure the pH value again to confirm no fluctuation.

10. The method for preparing a biomimetic sebum film moisturizing factor according to claim 6, characterized in that: In step S4, after the prepared solvent is cooled and stabilized, the following specific steps are taken to perform filtration to remove impurities and verify the final quality of the product: (1) Cooling and stabilizing operation: Add 25°C room temperature water to the constant temperature water bath to cool the stirring tank. Control the cooling rate to 1-2°C per minute. Keep stirring at 200 rpm during the cooling process. When the solution temperature drops to 25-28°C and is consistent with the room temperature, turn off the stirring, cover the stirring tank, and let it stand in a clean environment for 30 minutes to allow the component molecules to rearrange to a stable state. (2) Filtration and impurity removal operation to remove any possible residual small particles and ensure the purity of the finished product. Under aseptic conditions, the matured mixture is slowly filtered through a 0.22μm cellulose acetate microporous filter membrane into a sterile brown light-proof glass bottle. During the filtration process, the speed is kept uniform to avoid excessive pressure that could cause the filter membrane to rupture. If the filtration speed is too slow, the liquid level can be appropriately reduced to ensure thorough filtration. (3) Verify the final quality of the product and ensure that the product meets the technical requirements. Observe the finished product against a white background. It should be a transparent and uniform liquid without layering, sedimentation, or bubbles. It should meet the appearance requirements for use in skin care products. Insert a calibrated pH meter into the middle of the finished product to measure the pH value and confirm that it is still within the target value range. Finally, take 5 mL of the finished product and store it in a 4℃ refrigerator and a 45℃ oven for 24 hours. After taking it out, observe that there is no layering, discoloration, or odor. Only after all tests are qualified can the finished product be judged as completed.

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