Crocodile oil-containing striae gravidarum tightening and anti-wrinkle composition and application thereof

Abstract

The invention discloses a striae gravidarum tightening and anti-wrinkle composition containing alligator oil and application of the striae gravidarum tightening and anti-wrinkle composition. The composition provided by the invention is prepared from the following components in parts by mass: 3 to 20 parts of alligator oil, 0.3 to 3 parts of ceramide NP, 1 to 5 parts of silanol mannuronate, 1 to 8 parts of hydrolyzed collagen and 1 to 8 parts of herba centellae extract. The composition takes the alligator oil as a core active component, promotes collagen regeneration and repairs broken fibers; by compounding ceramide NP, the skin barrier is repaired, water loss is reduced, bionic liposome is formed, the transdermal absorption rate of active ingredients is increased, and the damaged barrier is supplemented; fibroblasts are directionally activated through silanol mannuronate, and I / III type collagen synthesis is promoted; the centella asiatica extract efficiently inhibits inflammatory factors and blocks scar hyperplasia, the hydrolyzed collagen promotes repair for filling and moisturizing, all the components have a synergistic effect, and the composition can be used for preparing external skin care products for preventing / repairing striae gravidarum and improving skin compactness.

Description

Technical Field

[0001] This invention relates to the field of medical skincare technology, and more specifically, to a firming and anti-wrinkle composition containing crocodile oil for stretch marks and its application. Background Technology

[0002] Stretch marks are essentially linear damage to the dermis, clinically manifesting as dark red or purplish-red streaks on the skin surface, which gradually fade into white or silvery-white atrophic scar-like lines. This skin problem has an incidence rate as high as 60%–90% in pregnant women, mainly distributed on the abdomen, but can also extend to the chest, back, buttocks, and proximal extremities. Stretch marks are atrophic scars caused by the rupture of elastic fibers in the dermis. Traditional repair products (such as ordinary moisturizers and olive oil) have poor penetration and insufficient active ingredients, making deep repair difficult.

[0003] Crocodile oil is rich in ω-3 / 6 / 9 fatty acids, squalene, vitamin E and natural antimicrobial peptides, and has the effects of promoting collagen regeneration, anti-inflammation and deep nourishment. However, since the formation of stretch marks is caused by the breakage of elastic fibers and collagen fibers in the dermis, if the ingredients can only stay on the epidermis, the effect will be very limited. Most of the oil components in crocodile oil mainly act on the epidermis to achieve the effects of sealing, moisturizing and softening. Therefore, the effect of crocodile oil alone is difficult to achieve a significant repair effect. The repair effect of stretch marks can be improved by adding other ingredients that have the ability to assist in penetration and repair. For example, a composition for repairing stretch marks disclosed in existing research contains crocodile oil, tocopherol, witch hazel and purslane. By combining crocodile oil with a variety of natural plant ingredients, the following problems still exist: (1) Low transdermal absorption rate: large molecular components are difficult to reach the dermis and the effect stays on the surface, which is only a temporary solution. (2) Unknown effective ingredients: the ingredients are complex, the concentration of active ingredients is unclear, the effect is unstable, and there are differences between batches. (3) Single mechanism of action: It focuses on moisturizing and anti-oxidation, and its ability to repair existing scars is not obvious. (4) It has little effect on white lines.

[0004] Therefore, existing stretch mark repair products primarily function to moisturize and hydrate the epidermis, failing to fundamentally repair broken dermal fibers. Their penetration is poor, resulting in limited repair effects. While some products that promote collagen production, such as those containing asiaticoside or rosehip oil (rich in retinoic acid precursors), exhibit slow and limited effects, there is a need to develop more products with better penetration and high efficacy for stretch mark repair, aiming to reshape the dermis, improve the appearance of stretch marks, and remove them. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the above-mentioned problems of existing stretch mark products. The present invention provides a stretch mark firming and anti-wrinkle composition containing crocodile oil and its application.

[0006] The first objective of this invention is to provide a firming and anti-wrinkle composition for stretch marks.

[0007] A second objective of this invention is to provide the application of a firming and anti-wrinkle composition for stretch marks.

[0008] A third objective of this invention is to provide a product.

[0009] The fourth objective of this invention is to provide a method for preparing the product.

[0010] The above-mentioned objective of this invention is achieved through the following technical solution: This invention provides a firming and anti-wrinkle composition for stretch marks, comprising the following components in parts by weight: 3-20 parts of crocodile oil, 0.3-3 parts of ceramide NP, 1-5 parts of silanol mannouronate, 1-8 parts of hydrolyzed collagen, and 1-8 parts of Centella asiatica extract.

[0011] The composition provided by this invention uses crocodile oil as the core active ingredient, combined with ceramide NP, silanol mannouronate, hydrolyzed collagen, and centella asiatica extract. This combination synergistically promotes collagen regeneration, repairs broken fibers and the skin barrier, and reduces moisture loss. Specifically, crocodile oil and ceramide form biomimetic liposomes, synergistically enhancing the transdermal absorption rate of the active ingredients and increasing permeability. Silanol mannouronate activates fibroblasts and stimulates collagen synthesis. Centella asiatica extract inhibits matrix metalloproteinase-1 (MMP-1) activity, reducing the rate of collagen breakage. Hydrolyzed collagen helps increase skin hydration and elasticity, and assists in improving fine lines. The components work synergistically to enhance the overall effect. This composition can be used to prepare more topical skincare products that repair / aid in the prevention and treatment of stretch marks and improve skin firmness, especially suitable for areas such as the abdomen and thighs. It can strengthen skin elasticity before pregnancy to achieve a preventative effect, deeply repair damaged skin barriers, firm and reduce wrinkles, promote elastic fiber regeneration, and provide immediate and long-lasting hydration. It has a long-term repair effect and is better suited for the repair of existing scars.

[0012] Preferably, the composition contains 5-15 parts of crocodile oil, 0.5-2 parts of ceramide NP, 1-4 parts of silanol mannouronate, 2-5 parts of hydrolyzed collagen, and 3-8 parts of Centella asiatica extract.

[0013] More preferably, the crocodile oil used in the composition is refined crocodile oil, which is prepared by taking crocodile abdominal fat, cleaning and removing impurities, freeze-drying, pulverizing and then sonicating at 20-50 kHz and 30-50 ℃, followed by supercritical CO2 extraction. After extraction, the initial crocodile oil is collected, stirred in a water bath at 40-55 ℃, and deodorized and decolorized. After vacuum filtration, refined crocodile oil is obtained.

[0014] In particular, the composition of this invention can use commercially available crocodile oil, which also has a good repair effect when combined with other components. Refined crocodile oil extracted through a specific preparation method, using supercritical CO2 low-temperature extraction, avoids the degradation of unsaturated fatty acids such as DHA, EPA, and oleic acid. The main fatty acids are concentrated in C16:0 (palmitic acid), C18:1 (monounsaturated fatty acid), and C18:2 (linoleic acid), with a ratio similar to the physicochemical properties of human fat, improving its absorption efficiency and resulting in better effects than commercially available products with unbalanced ratios. The preparation method provided by this invention can completely remove the unique fishy smell of crocodile oil, leaving only a slight oily odor. In contrast, most commercially available products have a strong fishy smell and require the addition of fragrances to mask it. The GC-MS scan of the refined crocodile oil in this invention identified only 16 distinct compounds within the range of 35–400 m / z, with no unknown chromatographic peaks. Combined with activated carbon filtration, this process effectively removes impurities such as pigments and odor substances. The refined crocodile oil prepared in this invention has a lighter and smoother texture, with a fluidity close to that of toner. It absorbs quickly and is non-sticky. Compared to commercially available products, which are often viscous and slow to absorb due to residual impurities, this method allows for better synergistic effects with other components, further enhancing its efficacy.

[0015] As a specific implementation scheme, the present invention provides a method for preparing the refined crocodile oil as follows: Fresh Nile crocodile abdominal fat (plate fat) is cleaned and impurities are removed, freeze-dried (vacuum degree 0.09 MPa, temperature -20℃), and then pulverized (4-120 mesh). The sample is then sonicated at 40 kHz and 40℃ for later use. The prepared sample is then placed in a reaction vessel, covered and tightened, and loaded into a reactor. The extraction temperature is set at 50℃ and the extraction pressure at 30 MPa. The CO2 flow rate (400 mL / min) is precisely controlled using an automated control system. After extraction for 2 hours, Nile crocodile oil (initial extraction) is collected. The initial extracted crocodile oil is stirred in a water bath at 40-55℃, followed by deodorization and decolorization treatment, and then vacuum filtered to obtain refined crocodile oil.

[0016] Preferably, the deodorization and decolorization treatment method adopts: activated carbon adsorption, steam distillation for deodorization, or ethanol-ethyl acetate azeotropic deodorization.

[0017] This invention provides the use of the above composition in the removal of stretch marks.

[0018] This invention provides the use of the above composition in the preparation of products for repairing / removing stretch marks or repairing damaged skin barriers.

[0019] The present invention also provides a product comprising the above-described composition.

[0020] Preferably, the product is a medical care product, dressing, cosmetic, etc.

[0021] Preferably, the product further contains emulsifiers, thickeners, co-emulsifiers, humectants, antioxidants, oil phase additives, preservatives, and / or pH adjusters.

[0022] Preferably, the glycoside emulsifier is selected from one or two of cetearyl glucoside and cocoyl glucoside; the thickener is selected from xanthan gum; the co-emulsifier is selected from cetearyl alcohol; the humectant is selected from one or two of glycerin and sodium hyaluronate; the antioxidant is selected from one or two of tocopherol and rosemary extract; the oil phase aid is selected from squalane; the preservative is selected from phenoxyethanol; and the pH adjuster is selected from triethanolamine.

[0023] More preferably, the glycoside emulsifier is cetearyl glucoside and cocoyl glucoside in a mass ratio of 1:1.

[0024] More preferably, the product contains the following components in weight percentage: 5-15 parts refined crocodile oil, 0.5-2 parts ceramide NP, 1-4 parts silanol mannouronate, 2-5 parts hydrolyzed collagen, 3-8 parts Centella asiatica extract, 1.5-2.5 parts cetearyl glucoside, 1.5-2.5 parts cocoyl glucoside, 0.5-1.0 parts cetearyl alcohol, 0.5-2 parts sodium hyaluronate, 1-3 parts tocopherol, 3-5 parts glycerin, 5-8 parts squalane, ≤0.8 parts phenoxyethanol, and the balance being deionized water.

[0025] This invention also provides a method for preparing the above-mentioned product, comprising the following steps: (1) Preparation of oil phase: Cetearyl glucoside, cocoyl glucoside, crocodile oil, ceramide, squalane, tocopherol, and cetearyl alcohol are mixed and heated to 75-80°C to obtain a transparent and homogeneous oil phase; (2) Preparation of aqueous phase: Xanthan gum and glycerol are pre-dispersed, then mixed with sodium hyaluronate and deionized water, heated to 75-80℃, and stirred to obtain aqueous phase; (3) Emulsification: Under stirring, the aqueous phase is added to the oil phase and stirred and homogenized to emulsify, resulting in a uniform emulsion; (4) Cooling and adding ingredients: When the emulsion is cooled to 40°C, add silanol mannouronic acid ester, hydrolyzed collagen and centella asiatica extract, mix and let the emulsion cool to below 30°C, adjust the pH, add preservatives, and stir to obtain the product.

[0026] As a specific implementation scheme, the present invention provides a specific method for preparing the above-mentioned product: (1) Preparation of oil phase: Cetearyl glucoside and cocoyl glucoside, refined crocodile oil, ceramide NP, squalane, tocopherol and cetearyl alcohol are mixed in proportion to form an oil phase, heated to 75-80℃, and stirred at 200-400rpm for 10-20min until completely dissolved to obtain a transparent and uniform oil phase; (2) Preparation of aqueous phase: After pre-dispersing xanthan gum and glycerol for 3-5 min, mix with sodium hyaluronate and deionized water to form an aqueous phase. Heat to 75-80℃ and stir at 400-600 rpm for 10-25 min until completely dissolved to obtain a transparent aqueous phase without particles; (3) Emulsification: Under stirring at 100-300 rpm, the aqueous phase is added to the oil phase at a flow rate of 3-5 mL / min. After stirring for 5-10 min, the mixture is homogenized at 1000-2500 rpm for 3-7 min to obtain a uniform emulsion. (4) Cooling and feeding: When the temperature is cooled to 30-50℃ at a rate of 0.5-1.5℃ / min, add silanol mannouronic acid ester, hydrolyzed collagen, centella asiatica extract and antioxidant, and stir at 100-200 rpm for 10-15 min; continue cooling to below 30℃, adjust the pH to 5.5-6.0 with triethanolamine, add phenoxyethanol, and stir for 3-5 min to obtain the product.

[0027] The present invention has the following beneficial effects: The composition provided by this invention uses crocodile oil as the core active ingredient, combined with ceramide NP, silanol mannouronate, hydrolyzed collagen, and centella asiatica extract. The resulting composition synergistically promotes collagen regeneration, repairs broken fibers and the skin barrier, and reduces moisture loss. Specifically, crocodile oil and ceramide form biomimetic liposomes, synergistically enhancing the transdermal absorption rate of the active ingredients and improving permeability. Silanol mannouronate can activate fibroblasts and stimulate collagen synthesis. Centella asiatica extract has anti-inflammatory and repairing effects, inhibiting scar hyperplasia. Hydrolyzed collagen helps increase skin hydration, elasticity, and improve fine lines. The composition provided by this invention addresses the issue that crocodile oil primarily acts on the epidermis, providing occlusive moisturizing and softening effects. The combined composition forms biomimetic liposomes, enhancing permeability, deeply repairing the damaged skin barrier, promoting elastic fiber regeneration and immediate moisturizing, and providing long-term repair effects. The composition provided by this invention can be used to prepare topical skin care products that prevent / repair stretch marks and improve skin firmness, and can be applied to the research and development of cosmetics, as well as to the medical aesthetics industry to prepare more medical consumables or base material raw materials. Attached Figure Description

[0028] Figure 1 The image shows the GC-MS analysis results of supercritical CO2 extraction of crocodile oil.

[0029] Figure 2 Image of HE-stained section.

[0030] Figure 3 Image of a Mosson-stained section. Detailed Implementation

[0031] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0032] Unless otherwise specified, all reagents and materials used in the following examples are commercially available.

[0033] Example 1 1. Preparation of refined crocodile oil (1) Take fresh Nile crocodile abdominal fat (plate fat), clean and remove impurities, freeze dry (vacuum degree 0.09 MPa, temperature -20℃), then pulverize (4~120 mesh), and sonicate at 40 kHz and 40 ℃ for later use; (2) Put the sample prepared above into the reaction vessel, cover and tighten it, put it into the reactor, set the extraction temperature to 50℃ and the extraction pressure to 30 MPa, use the automatic control system to precisely control the CO2 flow rate (400 mL / min) to introduce CO2, collect Nile crocodile oil (initial extraction) after extraction for 2 h, stir the initial extracted crocodile oil in a 40-55℃ water bath, then use activated carbon adsorption for deodorization and decolorization treatment, and then vacuum filter to obtain refined crocodile oil.

[0034] The refined crocodile oil obtained by the above supercritical CO2 extraction was analyzed by GC-MS, and the results are as follows: Figure 1 As shown, the absence of unknown impurity peaks and the low proportion of low-content peaks indicate that the supercritical CO2 extraction (50℃, 30 MPa) combined with activated carbon filtration can effectively remove short-chain impurities, structurally similar isomers, and pigments / odors. There are no solvent peaks, the baseline is stable, and there is no residual risk from traditional solvent extraction (such as n-hexane).

[0035] Further analysis was conducted on each identified compound, calculating its retention index (RI) and relative content (%), while also determining its fatty acid type and content. The results are shown in Tables 1 and 2. Table 1 shows that the core active ingredients (palmitic acid and linoleic acid) accounted for 28.2% (14.16% + 14.04%), while the content of low-value short-chain saturated fatty acids (such as C12 and C14) was <3%—indicating that this extraction process preferentially extracts beneficial medium- and long-chain fatty acids for the skin, avoiding the presence of ineffective impurities (such as low-boiling-point short-chain acids) in the composition. Table 2 shows that refined crocodile oil contains 25.44% monounsaturated fatty acids, including 13.16% oleic acid and 12.14% palmitoleic acid. Figure 1The three main peaks are unsaturated fatty acids (linoleic acid, C18:1), indicating that low-temperature supercritical extraction (≤60 ℃) avoids the degradation of unsaturated fatty acids (which are easily oxidized at high temperatures). The total amount of unsaturated fatty acids reaches 43.05%, of which essential fatty acids (mainly linoleic acid) account for 15.11%, which is 13% higher than that of commercially available ordinary crocodile oil (unsaturated fatty acids on average <30%), and its active efficacy will be superior.

[0036] In addition, refined crocodile oil contains 17.61% polyunsaturated fatty acids, with linoleic acid accounting for as much as 14.04%. As an essential fatty acid, it cannot be synthesized by the skin itself and must be obtained from external sources. At the same time, polyunsaturated fatty acids also have certain anti-inflammatory and antioxidant capabilities. In terms of their effects, on the one hand, supplementing with these essential fatty acids can maintain the integrity of the skin barrier and enhance the skin's water-locking ability, thereby indirectly improving fine lines; on the other hand, its antioxidant properties can reduce the damage of free radicals to skin cells, slow down the skin aging process, and thus help prevent the formation of new wrinkles.

[0037] Table 1. Retention index (RI) and relative content (%) of each compound identified in refined crocodile oil

[0038] Table 2. Fatty acid types and content of refined crocodile oil

[0039] 2. Preparation of the composition The composition provided in this embodiment contains the following components by weight: 5g refined crocodile oil, 0.5g ceramide NP, 1g silanol mannouronate, 2g hydrolyzed collagen, 3g Centella asiatica extract, 3g squalane, 0.5g sodium hyaluronate, 1.0g tocopherol, 2.5g cetearyl glucoside emulsifier, 2.5g cocoyl glucoside, 0.1g xanthan gum thickener, 3g glycerin, 0.8g cetearyl alcohol, 0.8g phenoxyethanol, and water as the balance (wherein cetearyl glucoside, cocoyl glucoside, xanthan gum, and glycerin are emulsion matrices and have no active effect).

[0040] The preparation method of this composition includes the following steps: (1) Preparation of oil phase: Mix the oil-soluble components in the formula: refined crocodile oil, squalane, tocopherol, ceramide, and cetearyl glucoside, heat to 70-75°C and stir until completely dissolved and homogeneous.

[0041] (2) Aqueous phase preparation: Xanthan gum and glycerin are stirred and dispersed evenly at room temperature. The water-soluble components in the formula, hydrolyzed collagen, sodium hyaluronate and Centella asiatica extract, are added to deionized water and heated to 70-75°C and stirred until completely dissolved.

[0042] (3) Emulsification: Use a homogenizer with a speed of 2000-3000 rpm to stir, then slowly add the aqueous phase to the oil phase and continue homogenizing for 3-5 minutes to form a uniform emulsion.

[0043] (4) Cooling: Switch to slow stirring (50 rpm) and begin cooling.

[0044] (5) Cooling and adding: Cool the emulsion to 38-42°C, add silanol mannouronic acid ester, and stir slowly for 15 minutes to mix it evenly.

[0045] (6) Filling: Continue cooling to below 30°C, discharge, fill, and obtain the finished product.

[0046] Example 2 The composition provided in this embodiment contains the following components by weight: 12g refined crocodile oil, 1.5g ceramide NP, 2.5g silanol mannouronate, 3g hydrolyzed collagen, 5g Centella asiatica extract, 6g squalane, 1.5g sodium hyaluronate, 2g tocopherol, 2.5g cetearyl glucoside emulsifier, 2.5g cocoyl glucoside emulsifier, 0.2g xanthan gum thickener, 5g glycerin, 0.8g cetearyl alcohol, 0.8g phenoxyethanol, and water as the balance. The preparation method of this composition is the same as in Example 1.

[0047] Example 3 The composition provided in this embodiment contains the following components by weight: 15g refined crocodile oil, 1.5g ceramide NP, 3g silanol mannouronate, 4g hydrolyzed collagen, 7g Centella asiatica extract, 8g squalane, 1.2g sodium hyaluronate, 2g tocopherol, 2.5g cetearyl glucoside emulsifier, 2.5g cocoyl glucoside emulsifier, 0.3g xanthan gum thickener, 5g glycerin, 0.8g cetearyl alcohol, 0.8g phenoxyethanol, and water as the balance. The preparation method of this composition is the same as in Example 1.

[0048] Example 4 The composition provided in this embodiment contains the following components by weight: 10g refined crocodile oil, 1.5g ceramide NP, 2.5g silanol mannouronate, 3g hydrolyzed collagen, 4g Centella asiatica extract, 6g squalane, 1g sodium hyaluronate, 1.5g tocopherol, 2.5g cetearyl glucoside emulsifier, 2.5g cocoyl glucoside emulsifier, 0.2g xanthan gum thickener, 5g glycerin, 0.8g cetearyl alcohol, 0.8g phenoxyethanol, and water as the balance. The preparation method of this composition is the same as in Example 1.

[0049] Example 5 The composition provided in this embodiment contains the following components by weight: 10g refined crocodile oil, 1.2g ceramide NP, 2.5g silanol mannouronate, 3.5g hydrolyzed collagen, 6g Centella asiatica extract, 5g squalane, 1.5g sodium hyaluronate, 1g tocopherol, 2.5g cetearyl glucoside emulsifier, 2.5g cocoyl glucoside emulsifier, 0.2g xanthan gum thickener, 4g glycerin, 0.8g cetearyl alcohol, 0.8g phenoxyethanol, and water as the balance. The preparation method of this composition is the same as in Example 1.

[0050] Example 6 The composition provided in this embodiment contains the following components by weight: 10g of commercially available crocodile oil, 1.2g of ceramide NP, 2.5g of silanol mannouronate, 3.5g of hydrolyzed collagen, 6g of Centella asiatica extract, 5g of squalane, 1.5g of sodium hyaluronate, 1g of tocopherol, 2.5g of emulsifier cetearyl glucoside, 2.5g of cocoyl glucoside, 0.2g of thickener xanthan gum, 4g of glycerin, 0.8g of cetearyl alcohol, 0.8g of phenoxyethanol, and the balance being water. The preparation method of this composition is the same as in Example 1.

[0051] Commercially available crocodile oil can be used in the composition, and it also exhibits good repairing effects when combined with other components. Refined crocodile oil, extracted through a specific preparation method, completely removes the characteristic fishy smell and pigments of crocodile oil, leaving only a slight oily odor. Its texture is lighter and smoother, with good fluidity and no stickiness, compared to commercially available crocodile oil, which is viscous and slowly absorbed due to residual impurities. The higher content of active ingredients in refined crocodile oil further enhances the active effects of the composition.

[0052] Comparative Example 1: Contains no crocodile oil The composition is the same as in Example 2, except that it does not contain crocodile oil.

[0053] Comparative Example 2: Contains no ceramides The composition is the same as in Example 2, except that it does not contain ceramides.

[0054] Comparative Example 3: Free of silanol mannourate The composition is the same as in Example 2, except that it does not contain silanol mannourate.

[0055] Comparative Example 4: Contains no other excipients. The composition is the same as in Example 2, except that it does not contain excipients such as squalane, sodium hyaluronate, tocopherol, thickener, emulsifier, glycerin, and phenoxyethanol, but only contains the main active ingredients: refined crocodile oil, ceramide NP, silanol mannouronate, hydrolyzed collagen, and centella asiatica extract.

[0056] Comparative Example 5: Excipients only The composition is the same as in Example 2, except that it contains only 6g of squalane, 1.5g of sodium hyaluronate, 2g of tocopherol, 2.5g of cetearyl glucoside, 0.2g of xanthan gum, 5g of glycerin, 0.8g of phenoxyethanol, and the balance being water. The preparation method of this composition is the same as in Example 1.

[0057] Comparative Example 6: Contains no hydrolyzed collagen or centella asiatica extract. The composition is the same as in Example 2, except that it contains only 12g of refined crocodile oil, 1.5g of ceramide NP, 2.5g of silanol mannouronate, 2.5g of cetearyl glucoside, 2.5g of cocoyl glucoside, 0.2g of xanthan gum, 5g of glycerin, 0.8g of cetearyl alcohol, 0.8g of phenoxyethanol, and the balance of water. The preparation method of this composition is the same as in Example 1.

[0058] Comparative Example 7: Free of refined crocodile oil, ceramides, and silanol mannouronate. The composition is the same as in Example 2, except that it contains only 3g of hydrolyzed collagen, 5g of Centella asiatica extract, 2.5g of cetearyl glucoside, 2.5g of cocoyl glucoside, 0.2g of xanthan gum, 5g of glycerin, 0.8g of cetearyl alcohol, 0.8g of phenoxyethanol, and the balance being water. The preparation method of this composition is the same as in Example 1.

[0059] Comparative Example 8: Contains no ceramides or silanol mannouronate. The composition is the same as in Example 2, except that it contains only 12g of refined crocodile oil, 3g of hydrolyzed collagen, 8g of Centella asiatica extract, 2.5g of cetearyl glucoside, 2.5g of cocoyl glucoside, 0.2g of xanthan gum, 5g of glycerin, 0.8g of cetearyl alcohol, 0.8g of phenoxyethanol, and the balance being water. The preparation method of this composition is the same as in Example 1.

[0060] Test Example 1: Cell Proliferation Experiment Collect fibroblasts in the logarithmic growth phase at a concentration of 4 × 10⁻⁶. 3 Cells were seeded at a density of 100 μL per well in 96-well plates and cultured for 24 h at 37°C with 5% CO2. Cells were then divided into a blank control group and a treatment group. The blank control group was treated with 100 μL of DMEM complete medium, while the treatment groups were treated with 100 μL of the compositions prepared in Examples 1-6 and Comparative Examples 1-8, each containing 50 μg / mL. Each group was divided into three replicates. Cells were cultured for another 48 h, and then 10 μL of CCK-8 solution was added to each well. After 2 h of further culture, the absorbance of each well was measured at 450 nm using a microplate reader, and the fibroblast viability was calculated.

[0061] Cell viability (%) = (average absorbance of the drug-treated group / average absorbance of the blank control group) × 100%.

[0062] The results are shown in Table 3. In the cell proliferation experiment, the fibroblast survival rate of the compositions prepared in Examples 1-6 was greater than 104%, which was higher than that of the control group. This indicates that none of the components affected the survival of fibroblasts, and the compositions prepared in Examples 1-6 promoted cell proliferation, significantly improving the fibroblast survival rate compared to the control group. Furthermore, compared to the blank group, the compositions in Examples 1-6 promoted fibroblast proliferation and demonstrated elastic fiber regeneration. Fibroblasts in white stretch marks are in a "low-activity state" and require activation by specific signaling molecules. Silanol mannouronate can bind to receptors on the fibroblast membrane through "glycosylation signals," activating intracellular "collagen synthesis pathways" (such as the PI3K-AKT pathway), directly promoting cell proliferation and collagen secretion. Cell experiment data showed that the formulation with this component (Example 2) achieved a fibroblast survival rate of 112.68%, while the control group (Example 3) without this component only achieved 103.21%, proving that it is key to activating fibroblasts. Hydrolyzed collagen is a small molecule peptide (molecular weight <1000Da), which directly provides fibroblasts with the "basic raw materials" for collagen synthesis; refined crocodile oil and ceramides optimize the environment, and the three work together to enable fibroblasts to "synthesize fibers and remodel the dermis".

[0063] Table 3. Fibroblast survival rate results of different compositions

[0064] Test Example 2: Evaluation of Anti-wrinkle Efficacy of Mouse Skin Animal model: BALB / c mice (female, 18–20 g) with their back hair shaved (2 × 3 cm area).

[0065] Ultraviolet radiation: UVB lamp (wavelength 280–320 nm), dose 180 mJ / cm² 2 Irradiation every other day for 4 weeks.

[0066] Model validation: Skin showed scaling and wrinkling after irradiation (clinical score ≥3 points).

[0067] Group treatment (n=8): Model group: 0.05% saline applied topically; Example 1 group: 0.05% solution applied topically; Comparative example 2 group: 0.05% solution applied topically; Positive control group: 0.05% retinoic acid cream applied topically; Normal control group: no irradiation + 0.05% saline applied topically; Each group was treated twice daily, with an interval of 6 hours (applied once before irradiation and once 6 hours after irradiation).

[0068] Testing indicators: Skin wrinkle score: 0 (smooth) to 4 (deep wrinkles). Specific mouse skin wrinkle scoring criteria (0-4 points) are as follows: 0 points: The skin surface is smooth, without any visible fine lines or wrinkles, consistent with the condition of normal, unexposed healthy skin; 1 point: Very slight fine lines appear on the skin surface, few in number and shallow, only visible at specific angles, without obvious protrusions or depressions, and the overall skin smoothness is good; 2 points: The skin shows obvious fine lines, which are more numerous and widely distributed. The depth of the fine lines is slightly deeper than that of 1 point. They can be clearly identified under normal observation angles, but no obvious wrinkles or depressions have formed. 3 points: The skin shows moderate to deep wrinkles with a certain depth and three-dimensionality. There are many wrinkles, and the unevenness of the skin surface can be observed directly. This score is also the critical standard for successful model verification. 4 points: The skin has deep groove-like wrinkles, which are deep and wide, with obvious depressions. The wrinkles are dense and have a strong three-dimensional effect. The skin has poor elasticity and is severely rough overall.

[0069] Histological analysis: Skin samples were taken from the back, and epidermal thickness was measured using HE staining. The collagen area ratio was calculated using Masson staining. The specific calculation formula is shown below: Average epidermal thickness of a single mouse (μm) = (sum of epidermal thicknesses across all fields of view) ÷ (total number of measurements). Collagen area ratio (%) = (Area of ​​Masson-positive areas in the dermis) ÷ (Total area of ​​the dermis) × 100%.

[0070] The results are shown in Table 4, indicating that the skin wrinkle score, epidermal thickness, and collagen area ratio of the mice in the UV irradiation model group were significantly increased. The skin wrinkle score, epidermal thickness, and collagen area ratio of the mice in Example 1 were all significantly better than those in the model group (…). P <0.01), close to the levels in the retinoic acid positive control group and the normal control group. HE staining and Masson staining results are as follows: Figure 2 and Figure 3As shown, HE staining, through hematoxylin staining of cell nuclei (blue) and eosin staining of cytoplasm / extracellular matrix (red), clearly allows for observation of structural changes such as epidermal thickness, dermal cell infiltration, and tissue integrity. HE staining results for the formulation group showed that the epidermal thickness was only 33.6 μm (close to the level of normal skin) compared to the 65.8 μm of the model group. The stratum corneum cells were arranged regularly without excessive keratinization or clefts, and the inflammatory area was significantly reduced. Masson staining stained collagen fibers blue and myofiber / cytoplasm red, allowing for a direct assessment of collagen content, arrangement regularity, and dermal thickness: the proportion of collagen area increased, and the dermal thickness recovered. Collagen fibers changed from "fragmented" to parallel bundle structures, which is highly consistent with the collagen arrangement pattern of normal skin (orderly distribution along the direction of skin tension).

[0071] Table 4 Anti-wrinkle results in model mice

[0072] Test Example 3: Transdermal Depth Test Four mL of the compositions formulated in Examples 1-6 and Comparative Examples 1-8 at a concentration of 1 mg / mL were mixed with 1 mL of fluorescein isothiocyanate at a concentration of 1 mg / mL and reacted overnight. The mixture was then dialyzed overnight in a dialysis bag with a molecular weight cutoff of 14000 Da to remove unreacted small molecules, yielding the fluorescently labeled composition. Fresh pig skin was washed with PBS, and the cuticle was fixed upwards in a transdermal diffusion apparatus and incubated at 37°C. 0.5 mL of the fluorescently labeled complex solution at a concentration of 1 mg / mL was dropped onto the pig skin tissue. After 24 hours, frozen sections were prepared, and the permeation of the composition was observed under a confocal microscope.

[0073] The penetration depth statistics are shown in Table 5. The penetration depths of Examples 1-6 are concentrated between 81±5 and 108±6 μm, generally falling within the range of "deep epidermis to superficial dermis". In contrast, the comparative group, lacking key penetration-enhancing components, exhibited poorer penetration depth and decreased transdermal stability. Only a composition formulation simultaneously containing "biomimetic liposomes (crocodile oil + ceramide NP + squalane) + small molecule active ingredients (silanol mannouronate) + mild excipients (glycoside emulsifiers)" can achieve the best penetration depth and high stability in the superficial dermis.

[0074] Table 5. Transdermal penetration depth of the composition after 24 hours

[0075] Test Example 4: Stretch Mark Removal Test 1. Experimental Methods: Seventy healthy women aged 25–42 years were selected on a voluntary basis. All participants were more than three months postpartum, had noticeable stretch marks around their abdomen, and had no serious systemic diseases, immunodeficiency, autoimmune diseases, active allergic diseases, a history of severe allergies to skincare cosmetics, or had received any other stretch mark treatments within the past year. Participants were prohibited from applying any medications or cosmetics unrelated to the experiment during the trial.

[0076] Seventy volunteer subjects were randomly divided into 14 groups, one group for each of Examples 1-6 and Comparative Examples 1-8, with 5 subjects in each group. The trial procedure involved applying the corresponding composition sample (prepared to a consistent concentration) to the skin areas with stretch marks after cleansing the abdominal skin in the morning and evening. The dosage was 5-10g per application, followed by 5 minutes of massage after each application, twice a day, for 3 consecutive months.

[0077] 2. Evaluation Criteria: The effectiveness of the stretch mark repair cream will be evaluated based on user experience using a scoring system. The evaluation will consider five aspects: the degree to which stretch marks are lightened, the degree to which the area of ​​stretch marks is reduced, the degree to which the skin's normal color is restored, and its nourishing and moisturizing properties. Specific evaluation details are as follows: (1) Degree of fading of stretch marks, degree of reduction in the area of ​​stretch marks, degree of skin restoration to normal skin color (0-5 points for each item): 5 stars: Completely satisfied, stretch marks have completely disappeared, and skin color has returned to normal. 4.5-5 points: Very satisfied, stretch marks have almost completely disappeared, and skin color is close to normal skin color; 4-4.5 points: Very satisfied, the stretch marks have faded significantly and most of them have disappeared, and the skin color is close to the normal skin color; 3-4 points: Acceptable, stretch marks have faded to some extent, some have disappeared, and skin color has become lighter; 3 points or below: Unacceptable; the color, extent, and skin color of the stretch marks have hardly changed.

[0078] (2) Nourishing and moisturizing properties (0-2.5 points each): 2.5 stars: Completely satisfied; 2.25-2.5 points: Very satisfied; 2.00-2.25 points: Very satisfied; 1.5-2.0 points: Acceptable; Below 1.5 points: Not good, unacceptable.

[0079] (3) The total score for the five indicators is 20 points, and the evaluation criteria are as follows: A total score of 16 or above indicates that the subjects were relatively satisfied, suggesting that the sample repair effect was good.

[0080] Total score 12-16: The subjects found it acceptable, indicating that the sample had a certain repair effect.

[0081] Total score 0-12: Subjects could not accept this, indicating that the sample had no obvious repair effect.

[0082] The results are shown in Table 6. The scoring results in the table show that groups 1-6 of Examples all achieved high scores in five indicators: the degree of fading of stretch marks, the degree of reduction in the area of ​​stretch marks, the degree of skin restoration to normal skin color, and nourishing and moisturizing properties. The average total score for groups 1-5 was between 17 and 18, all exceeding 80% of the full score. This indicates that the subjects were quite satisfied with the effects of these compositions, which demonstrated good repair effects, particularly on existing scars, and also showed good repair effects on stretch marks.

[0083] However, the average total score for Comparative Example 1 was 13.65, and the average total score for Comparative Example 2 was 13.21. This indicates that the subjects found the effects of the compositions used in the comparative examples acceptable and that they had some restorative effect. However, these two values ​​are close to 12 (60% of the full score), suggesting that the restorative effect of the comparative examples was only at a moderate level.

[0084] It is worth noting that, with the same total amount of active ingredients, Comparative Example 1, which did not use crocodile oil, and Comparative Example 2, which did not contain ceramides, were less effective than Examples 1-6. This demonstrates that the synergistic effect of crocodile oil and ceramides cannot be ignored. The appropriate ratio of the two ingredients significantly improves the effect compared to using either one alone. Overall, the compositions of Examples 1-6 effectively improve the overall repair of stretch marks, the area of ​​stretch marks, the color of stretch marks, and the laxity of stretch marks.

[0085] Table 6. Comprehensive Evaluation Scores of Stretch Mark Repair Efficacy Test

[0086] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A firming and anti-wrinkle composition for stretch marks, characterized in that, It contains the following components by weight: 3-20 parts crocodile oil, 0.3-3 parts ceramide NP, 1-5 parts silanol mannouronate, 1-8 parts hydrolyzed collagen, and 1-8 parts centella asiatica extract.

2. The composition according to claim 1, characterized in that, The composition contains 5-15 parts of crocodile oil, 0.5-2 parts of ceramide NP, 1-4 parts of silanol mannouronate, 2-5 parts of hydrolyzed collagen, and 3-8 parts of Centella asiatica extract.

3. The composition according to claim 1 or 2, characterized in that, The crocodile oil used is refined crocodile oil, and its preparation method is as follows: take the abdominal fat of crocodiles, clean and remove impurities, freeze dry, pulverize and then sonicate at 20-50 kHz and 30-50℃, followed by supercritical CO2 extraction. After extraction, collect the initial crocodile oil, stir it in a water bath at 40-55℃, and perform deodorization and decolorization treatment. After vacuum filtration, refined crocodile oil can be obtained.

4. The use of the composition according to any one of claims 1 to 3 in the removal of stretch marks.

5. The use of the composition according to any one of claims 1 to 3 in the preparation of products for repairing / removing stretch marks or repairing damaged skin barriers.

6. A product characterized in that, The composition comprising any one of claims 1 to 3.

7. The product according to claim 6, characterized in that, The product also contains one or more of the following: glycoside emulsifiers, thickeners, co-emulsifiers, humectants, antioxidants, oil phase additives, preservatives, and pH adjusters.

8. The product according to claim 7, characterized in that, The glycoside emulsifier is selected from one or two of cetearyl glucoside and cocoyl glucoside; the thickener is selected from xanthan gum; the co-emulsifier is selected from cetearyl alcohol; the humectant is selected from one or two of glycerin and sodium hyaluronate; the antioxidant is selected from one or two of tocopherol and rosemary extract; the oil phase aid is selected from squalane; the preservative is selected from phenoxyethanol; and the pH adjuster is selected from triethanolamine.

9. The product according to claim 8, characterized in that, The product also contains the following components in parts by weight: 1.5-2.5 parts cetearyl glucoside, 1.5-2.5 parts cocoyl glucoside, 0.5-1.0 parts cetearyl alcohol, 0.5-2 parts sodium hyaluronate, 1-3 parts tocopherol, 3-5 parts glycerin, 0.1-0.3 parts xanthan gum, 5-8 parts squalane, ≤0.8 parts phenoxyethanol, and the balance being deionized water.

10. The method for preparing the product according to claim 9, characterized in that, Includes the following steps: (1) Preparation of oil phase: Cetearyl glucoside, cocoyl glucoside, crocodile oil, ceramide, squalane, tocopherol and cetearyl alcohol are mixed and heated to 75-80℃ to obtain a transparent and homogeneous oil phase; (2) Preparation of aqueous phase: Xanthan gum and glycerol are pre-dispersed, then mixed with sodium hyaluronate and deionized water, heated to 75-80℃, and stirred to obtain aqueous phase; (3) Emulsification: Under stirring, the aqueous phase is added to the oil phase and stirred and homogenized to emulsify, resulting in a uniform emulsion; (4) Cooling and adding ingredients: When the emulsion is cooled to 40°C, add silanol mannouronic acid ester, hydrolyzed collagen and centella asiatica extract, mix and let the emulsion cool to below 30°C, adjust the pH, add preservatives, and stir to obtain the product.

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