Separation equipment for stem cell culture

Abstract

The invention provides separation equipment for stem cell culture, and relates to the technical field of cell culture.The separation equipment comprises a base, a separation cylinder is fixedly mounted on the upper side of the base, a centrifugal assembly for separating stem cells is mounted in the separation cylinder, and a driving assembly is arranged in the middle of the base; an auxiliary assembly used for storing culture dishes is fixedly installed on one side of the separation barrel, a PLC is installed on one side of the base, a supporting frame is fixedly installed on the other side of the base, a sliding rail is arranged in the supporting frame, an overturning seat is movably arranged in the sliding rail, a turning cover is fixedly installed at one end of the overturning seat, and a lifting rod is fixedly installed on the turning cover. A sealing ring is arranged on the outer side of the turning cover, and the turning cover is arranged at the upper end of the separation cylinder. Compared with existing separation equipment needing manual extraction of the stem cells, the stem cells can be automatically and continuously extracted through the stem cell extraction device, the layered stem cells are separated, the separation efficiency can be improved under the condition that a large number of stem cells are separated, and the workload of operators is reduced.

Description

Technical Field

[0001] This invention relates to the field of cell culture technology, and more specifically, to a separation device for stem cell culture. Background Technology

[0002] In the field of stem cell culture, stem cell separation is a crucial operation, and its separation effect directly affects the quality and efficiency of subsequent stem cell culture. For example, a stem cell separation device with patent publication number CN219702274U includes a separation cylinder, a drive motor, and a separation disk. The separation cylinder and the separation disk are equipped with a rotation mechanism, and the separation disk is equipped with several separation tubes. The drive motor is fixedly installed at the bottom axis of the separation cylinder, and the separation disk is fixedly installed at one end of the output shaft of the drive motor. After centrifugation, the aforementioned separation equipment requires operators to rely on their experience and visual judgment to determine the stratified positions of stem cells before manually extracting them. In large-scale stem cell separation, this manual, continuous extraction method presents several problems. Firstly, prolonged manual operation can lead to operator fatigue, reducing the accuracy of determining stem cell stratification and thus affecting the separation effect. Secondly, manual extraction is inefficient and cannot meet the speed requirements of large-scale stem cell culture. Therefore, this application designs a stem cell culture separation device that reduces operator workload through automated separation, thereby improving separation efficiency in situations requiring large-scale separation. Summary of the Invention

[0003] To address the shortcomings of existing technologies, this invention provides a separation device for stem cell culture, which solves the problem that existing manual extraction operations are inefficient and cannot meet the needs of large-scale stem cell separation.

[0004] To achieve the above objectives, the technical solution adopted by the present invention is as follows: A stem cell culture separation device includes a base, a separator cylinder fixedly mounted on the upper side of the base, a centrifugation component for separating stem cells installed inside the separator cylinder, a drive component disposed in the middle of the base, an auxiliary component for storing culture dishes fixedly mounted on one side of the separator cylinder, a PLC controller mounted on one side of the base, a support frame fixedly mounted on the other side of the base, a slide rail disposed inside the support frame, a flip seat movably disposed inside the slide rail, a flip cover fixedly mounted on one end of the flip seat, a sealing ring disposed on the outer side of the flip cover, and the flip cover being disposed on the upper end of the separator cylinder.

[0005] Preferably, a rotating groove is provided on one side of the flip cover, a rotating frame is rotatably installed inside the rotating groove, a transfer groove is provided at the end of the rotating frame, a squeezing wheel is provided inside the transfer groove, an exchange channel is provided at the upper and lower ends of the rotating groove, a sealing seat is provided at the end of each exchange channel, a delivery hose is installed between two sealing seats, a drain pipe is fixedly installed at the upper sealing seat, a liquid collection needle is fixedly installed at the lower sealing seat, and the two ends of the delivery hose are connected to the drain pipe and the liquid collection needle, respectively.

[0006] Preferably, a mounting groove is provided on one side of the rotating groove, and a drive motor is fixedly installed inside the mounting groove. The output end of the drive motor extends to the rotating groove and is fixedly connected to the middle of the rotating frame. A liquid storage box is installed on the upper surface of the flip cover. An extension pipe is provided at the drain end of the liquid storage box. The extension pipe is connected to the body of the drain pipe. A first solenoid valve is installed on the body of the extension pipe.

[0007] Preferably, the drive assembly includes a drive motor, which is fixedly installed inside one side of the base. The partition cylinder has several transmission slots inside, one of which has a drive gear rotatably installed inside, and the other two have mating gears rotatably installed inside. The output end of the drive motor is fixedly connected to the drive gear.

[0008] Preferably, a boss is fixedly installed on one side of the base, an electric actuator and a hydraulic chamber are fixedly installed on the upper end of the boss, a piston rod is fixedly installed at the output end of the electric actuator, the piston rod is located inside the hydraulic chamber, a transfer pipe is installed at one end of the hydraulic chamber, and a second solenoid valve is installed on the body of the transfer pipe.

[0009] Preferably, the centrifugal assembly includes a fixed cylinder, which is fixedly installed at the center of the separator. A rotating sleeve is rotatably mounted inside the fixed cylinder via a rotary bearing. A driven gear is provided on the outer side of the rotating sleeve, and the driven gear meshes with a driving gear and a mating gear. A hydraulic groove is provided inside the rotating sleeve, and a splined shaft is movably and sealed inside the hydraulic groove. A piston is provided at the bottom of the splined shaft, and a mounting plate is provided at the upper end of the splined shaft. Several extension frames are mounted on the outer side of the mounting plate, and a rotating seat is provided at the outer end of the extension frame. A placement seat is rotatably mounted inside each rotating seat.

[0010] Preferably, the upper end of the adapter tube extends to the bottom of the hydraulic tank and is rotatably engaged, a plane bearing is installed between the bottom of the rotating sleeve and the body of the adapter tube, and a sealed bearing is provided between the body of the adapter tube and the hydraulic tank.

[0011] Preferably, the placement base has light-transmitting grooves at both ends, a column is fixedly installed on one side of the bottom of the partition cylinder, an optical sensor receiver is fixedly installed on one side of the column, an optical sensor transmitter is fixedly installed on one side of the inner wall of the partition cylinder, and the optical sensor transmitter and optical sensor receiver are electrically connected to the PLC controller. The storage tube is placed inside the placement seat. The storage tube is covered with a top cover. A sealing gasket is provided on the lower side of the top cover. A perforation is provided in the middle of the top cover.

[0012] Preferably, the column has a movable groove inside, and a movable rod is movably installed inside the movable groove by means of a support spring. A limit frame is installed at one end of the movable rod.

[0013] Preferably, the auxiliary component includes a placement frame, a control motor is fixedly installed on the upper end of the placement frame, a turntable is fixedly installed on the output end of the control motor, a plurality of positioning frames for placing culture dishes are provided on the surface of the turntable, a receiving box is fixedly installed on the lower side of the turntable, and a flow guide groove is opened inside the turntable, the flow guide groove is connected to the receiving box.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. By starting the drive motor, the rotating frame can be rotated, and the positions of several extrusion rollers can be switched. The extrusion rollers can continuously extrude the delivery hose, so that the liquid discharge operation can be carried out in the corresponding direction according to the forward and reverse rotation direction of the drive motor. The liquid collection needle can be inserted into the storage test tube through the perforation and through the sealing gasket. After entering, the stem cells are extracted by the drive motor. Compared with the existing separation equipment that requires manual extraction of stem cells, this application can automatically and continuously extract stem cells, realize the separation of stratified stem cells, and improve the separation efficiency and reduce the workload of operators when separating a large number of stem cells.

[0015] 2. By setting up a storage tank to store cleaning liquid, after a single extraction and separation is completed, the first solenoid valve is opened, and the drive motor is started to rotate in both directions sequentially. This allows the cleaning liquid inside the storage tank to be discharged from the drain tube and the retrieval needle tube respectively, ensuring that no stem cells remain inside the delivery tubing, drain tube, and retrieval needle tube after a single extraction and separation. The cleaning liquid discharged from the drain tube is collected and processed by the auxiliary components, while the cleaning liquid discharged from the retrieval needle tube is stored in the currently separated storage test tube, which is beneficial for centralized waste disposal later. Through the above structure, this application can avoid cross-contamination of stem cells in different storage test tubes during continuous separation. While achieving efficient separation, it improves the integrity of single stem cell extraction. Compared with the prior art where operators use pipettes for mixed extraction, this application can reduce the cross-contamination of stem cells during pipette transfer.

[0016] 3. By activating the electric actuator, the position of the piston rod inside the hydraulic chamber can be adjusted, thereby controlling the delivery of hydraulic fluid. The hydraulic fluid enters the hydraulic tank through the transfer pipe. Under the action of hydraulic pressure, the spline shaft is lifted upward, which in turn drives the outer peripheral placement seat to adjust its height. This ensures that the liquid extraction needle enters different positions inside the storage test tube, enabling precise extraction of different layers of substances inside the storage test tube.

[0017] The above structure allows for centrifugation of stem cells to separate them into layers, and the extraction height can be adjusted to ensure precise extraction of stem cells from the corresponding layers, avoiding the extraction of substances from other layers. Attached Figure Description

[0018] Figure 1 This is a three-dimensional structural schematic diagram of the present invention; Figure 2 This is a three-dimensional structural schematic diagram from another perspective of the present invention; Figure 3 This is a top view of the structure of the present invention; Figure 4 yes Figure 3 Schematic diagram of the cross-sectional structure at point AA; Figure 5 This is a three-dimensional structural diagram of the separator cylinder; Figure 6 This is a three-dimensional structural diagram of the partitioned cylindrical structure from another perspective; Figure 7 This is a top view of the partition cylinder structure. Figure 8 yes Figure 7 Schematic diagram of the cross-sectional structure at point BB; Figure 9 yes Figure 7 Schematic diagram of the cross-sectional structure at the CC section; Figure 10 yes Figure 9 Enlarged structural diagram at point a; Figure 11 This is a side view of the partition cylinder structure. Figure 12 yes Figure 11 Schematic diagram of the cross-sectional structure at point DD; Figure 13 yes Figure 11 Schematic diagram of the cross-sectional structure at the middle EE section; Figure 14 This is a three-dimensional structural diagram of the flip phone; Figure 15 This is a three-dimensional structural diagram of the flip phone from another perspective; Figure 16This is a top view of the flip phone's structure. Figure 17 yes Figure 16 Enlarged structural diagram at point b; Figure 18 yes Figure 16 Schematic diagram of the cross-sectional structure at the FF section.

[0019] In the diagram: 1. Base; 2. Divider cylinder; 3. Support frame; 301. Slide rail; 4. Flip cover; 401. Flip seat; 402. Sealing ring; 403. Liquid storage box; 4031. Extension tube; 4032. First solenoid valve; 404. Installation slot; 4041. Drive motor; 405. Drain pipe; 406. Liquid collection needle tube; 407. Sealing seat; 408. Rotating slot; 409. Rotating frame; 4091. Adapter slot; 4092. Extrusion wheel; 410. Delivery hose; 411. Exchange channel; 5. Drive assembly; 501. Boss; 502. Drive motor; 5021. Drive gear; 5022. Transmission slot; 5023. Matching gear; 503. Electric actuator; 5031. Piston actuator; 5032. Hydraulic chamber; 5033. Second solenoid valve; 5034. Adapter pipe; 6. Auxiliary 601. Auxiliary components; 602. Mounting frame; 603. Control motor; 604. Turntable; 605. Positioning frame; 606. Guide channel; 607. Receiver box; 7. PLC controller; 8. Centrifuge assembly; 801. Fixed cylinder; 8011. Rotary bearing; 8012. Surface bearing; 8013. Driven gear; 802. Rotating sleeve; 8021. Hydraulic groove; 8022. Splined shaft; 803. Mounting plate; 804. Extension frame; 8041. Rotating seat; 8042. Placement seat; 8043. Light transmission groove; 9. Storage test tube; 901. Top cover; 902. Sealing gasket; 903. Perforation; 10. Column; 1001. Movable groove; 1002. Support spring; 1003. Movable rod; 1004. Limiting frame; 11. Optical sensor transmitter; 12. Optical sensor receiver. Detailed Implementation

[0020] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0021] like Figures 1 to 3As shown, a stem cell culture separation device includes a base 1, a separator 2 fixedly mounted on the upper side of the base 1, a centrifugation component 8 for separating stem cells installed inside the separator 2, a drive component 5 disposed in the middle of the base 1, an auxiliary component 6 for storing culture dishes fixedly mounted on one side of the separator 2, a PLC controller 7 mounted on one side of the base 1, and a support frame 3 fixedly mounted on the other side of the base 1. A slide rail 301 is disposed inside the support frame 3, and a flip seat 401 is movably disposed inside the slide rail 301. A flip cover 4 is fixedly mounted at one end of the flip seat 401, and a sealing ring 402 is disposed on the outer side of the flip cover 4. The flip cover 4 is located at the upper end of the separator 2. Through the movable connection between the slide rail 301 and the flip seat 401, the flip cover 4 can be flipped and moved up and down, allowing the operator to open the flip cover 4 when placing the storage test tube 9 and close the flip cover 4 when separation is needed. The PLC controller 7 can control the start and stop of the drive motor 502, electric actuator 503, solenoid valve, drive motor 4041 and control motor 602, and can receive signals from optical sensors to trigger the start and stop adjustment of the control electric actuator 503.

[0022] like Figures 1 to 4 , Figure 9 , Figures 14 to 18 As shown, a rotating groove 408 is provided on one side of the flip cover 4. A rotating frame 409 is rotatably mounted inside the rotating groove 408. A transfer groove 4091 is provided at the end of the rotating frame 409. A squeezing wheel 4092 is provided inside the transfer groove 4091. Exchange channels 411 are provided at the upper and lower ends of the rotating groove 408, respectively. A sealing seat 407 is provided at the end of each exchange channel 411. A delivery hose 410 is installed between two sealing seats 407. A drain pipe 405 is fixedly installed on the upper sealing seat 407, and a liquid-taking needle tube 406 is fixedly installed on the lower sealing seat 407. The two ends of the delivery hose 410 are connected to the drain pipe 405 and the liquid-taking needle tube 406, respectively. The delivery hose 410 can be replaced according to the usage. In this embodiment, a mounting groove 404 is provided on one side of the rotating groove 408, and a drive motor 4041 is fixedly installed inside the mounting groove 404. The output end of the drive motor 4041 extends to the rotating groove 408 and is fixedly connected to the middle of the rotating frame 409. A liquid storage box 403 is installed on the upper surface of the flip cover 4. An extension pipe 4031 is provided at the drain end of the liquid storage box 403. The extension pipe 4031 is connected to the body of the drain pipe 405. A first solenoid valve 4032 is installed on the body of the extension pipe 4031.

[0023] By activating the drive motor 4041, the rotating frame 409 can be rotated, switching the positions of several extrusion rollers 4092. The extrusion rollers 4092 can continuously extrude the delivery hose 410, thus enabling liquid discharge operations in the corresponding direction according to the forward and reverse rotation direction of the drive motor 4041. The liquid collection needle 406 can pass through the perforation 903 and the sealing gasket 902 to enter the interior of the storage test tube 9. After entering, the drive motor 4041 is used to extract stem cells. Compared with existing separation equipment that requires manual extraction of stem cells, this application can automatically and continuously extract stem cells, realizing the separation of stratified stem cells. Under the separation of a large number of stem cells, this application can improve the separation efficiency and reduce the workload of operators.

[0024] The sealing gasket 902 is for single use only and must be replaced after separation when cleaning the storage tube 9. The storage box 403 stores cleaning liquid. After a single extraction and separation is completed, the first solenoid valve 4032 is opened, and the drive motor 4041 is started to rotate in both directions. This allows the cleaning liquid inside the storage box 403 to be discharged from the drain pipe 405 and the retrieval needle 406, respectively. This ensures that the delivery hose 410, drain pipe 405, and retrieval needle 406 do not contain any stem cells after a single extraction and separation. The cleaning liquid discharged from the drain pipe 405 is collected and processed by the auxiliary component 6, while the cleaning liquid discharged from the retrieval needle 406 is stored in the currently separated storage tube 9, which is beneficial for centralized waste disposal later. This structure can prevent cross-contamination of stem cells in different storage tubes 9 during continuous separation. It improves the integrity of a single stem cell extraction while achieving efficient separation. Compared with the prior art where operators use pipettes for mixed extraction, this application can reduce the cross-contamination of stem cells during pipette transfer.

[0025] like Figure 1 , Figures 6 to 17 As shown, the drive assembly 5 includes a drive motor 502, which is fixedly installed inside one side of the base 1. Several transmission slots 5022 are opened inside the partition cylinder 2. A drive gear 5021 is rotatably installed inside one of the transmission slots 5022, and a mating gear 5023 is rotatably installed inside the other two transmission slots 5022. The output end of the drive motor 502 is fixedly connected to the drive gear 5021.

[0026] It should be noted that a boss 501 is fixedly installed on one side of the base 1. An electric actuator 503 and a hydraulic chamber 5032 are fixedly installed on the upper end of the boss 501. A piston rod 5031 is fixedly installed at the output end of the electric actuator 503. The piston rod 5031 is located inside the hydraulic chamber 5032. A transfer pipe 5034 is installed at one end of the hydraulic chamber 5032. A second solenoid valve 5033 is installed on the body of the transfer pipe 5034.

[0027] By starting the drive motor 502, the drive gear 5021 can be driven to rotate. The use of the mating gear 5023 makes the drive gear 5021 more stable when meshing with the driven gear 8013. The meshing of the drive gear 5021 and the driven gear 8013 allows the rotating sleeve 802 to rotate. The limiting fit between the spline shaft 8022 and the rotating sleeve 802 drives the mounting plate 803 to rotate, thereby centrifuging the storage test tube 9 stored inside the placement seat 8042, achieving stratification inside the storage test tube 9, and realizing the separation of stem cells.

[0028] By activating the electric actuator 503, the position of the piston actuator 5031 inside the hydraulic chamber 5032 can be adjusted, thereby controlling the delivery of hydraulic fluid. The hydraulic fluid enters the hydraulic tank 8021 through the adapter pipe 5034. Under the action of hydraulic pressure, the spline shaft 8022 is lifted upward, which in turn drives the outer peripheral placement seat 8042 to adjust its height. This ensures that the liquid extraction needle 406 enters different positions inside the storage test tube 9, enabling precise extraction of different layered substances inside the storage test tube 9.

[0029] The above structure allows for centrifugation of stem cells to separate them into layers, and the extraction height can be adjusted to ensure precise extraction of stem cells from the corresponding layers, avoiding the extraction of substances from other layers.

[0030] In this application, the centrifugal assembly 8 includes a fixed cylinder 801, which is fixedly installed at the center of the separator cylinder 2. Inside the fixed cylinder 801, a rotating sleeve 802 is rotatably installed via a rotating bearing 8011. A driven gear 8013 is provided on the outside of the rotating sleeve 802. The driven gear 8013 meshes with the driving gear 5021 and the mating gear 5023. A hydraulic groove 8021 is opened inside the rotating sleeve 802. A spline shaft 8022 is movably and sealed inside the hydraulic groove 8021. A piston is provided at the bottom of the spline shaft 8022. A mounting plate 803 is provided at the upper end of the spline shaft 8022. Several extension frames 804 are installed on the outside of the mounting plate 803. A rotating seat 8041 is provided at the outer end of the extension frame 804. A placement seat 8042 is rotatably installed inside each rotating seat 8041. The placement seat 8042 is rotatably mounted inside each rotating seat 8041, which allows the placement seat 8042 to swing to a certain extent when the mounting plate 803 rotates centrifugally, which is beneficial to the centrifugation effect.

[0031] The upper end of the adapter pipe 5034 extends to the bottom of the hydraulic tank 8021 and is rotatably engaged. A flat bearing 8012 is installed between the bottom of the rotating sleeve 802 and the body of the adapter pipe 5034, and a sealed bearing is provided between the body of the adapter pipe 5034 and the hydraulic tank 8021. The sealed bearing between the body of the adapter pipe 5034 and the hydraulic tank 8021 ensures that the adapter pipe 5034 does not interfere with the rotation of the rotating sleeve 802, and that the operation between the rotating sleeve 802 and the adapter pipe 5034 is sealed when conveying hydraulic fluid, thus guaranteeing the stability of the hydraulic support.

[0032] In the specific setup, the placement seat 8042 has light-transmitting slots 8043 at both ends. A column 10 is fixedly installed on one side of the bottom of the separator cylinder 2, and an optical sensor receiver 12 is fixedly installed on one side of the column 10. An optical sensor transmitter 11 is fixedly installed on one side of the inner wall of the separator cylinder 2. The optical sensor transmitter 11 and the optical sensor receiver 12 are electrically connected to the PLC controller 7. During the extraction and separation process, one of the placement seats 8042 will be located between the optical sensor transmitter 11 and the optical sensor receiver 12. A light beam is generated through the optical sensor transmitter 11, and the optical sensor receiver 12 receives the light beam. The light beam can pass through the light-transmitting groove 8043 and then through the storage tube 9 to determine the internal layering of the storage tube 9. The upper clear liquid layer has fewer impurities and higher light transmittance, while the lower layer has more impurities and lower light transmittance. When the light transmittance is significantly reduced, the PLC controller 7 controls the electric push rod 503 to stop injecting hydraulic fluid, thus fixing the current height of the storage tube 9. When the optical sensor receiver 12 detects the impurity layer, the current height of the liquid extraction needle 406 is located at the middle layer of stem cells, and then precise stem cell extraction can be performed, realizing automatic extraction and separation operation without the need for personnel to observe and manually extract stem cells, thus improving separation efficiency.

[0033] The storage tube 9 is placed inside the placement base 8042. A top cover 901 is installed on the upper end of the storage tube 9, and a sealing gasket 902 is provided on the lower side of the top cover 901. A perforation 903 is opened in the middle of the top cover 901. The sealing gasket 902 ensures the sealing performance of the storage tube 9 before separation, and the sealing gasket 902 can be passed through by the liquid extraction needle 406, so that the liquid extraction needle 406 can enter the storage tube 9 during separation to achieve extraction and separation. After separation is completed, the storage tube 9 and the top cover 901 can be reused after cleaning and replacing the sealing gasket 902.

[0034] The column 10 has a movable groove 1001 inside, and a movable rod 1003 is movably mounted inside the movable groove 1001 via a support spring 1002. A limit frame 1004 is installed at one end of the movable rod 1003. The movable rod 1003 is supported by the support spring 1002 so that when the placement seat 8042 approaches the limit frame 1004, it will be limited by the limit frame 1004. This ensures that when switching the placement seat 8042 for continuous extraction and separation, the positions of the placement seat 8042 and the light-transmitting groove 8043 can be precisely aligned with the optical sensor transmitter 11 and the optical sensor receiver 12.

[0035] It should be noted that the auxiliary component 6 includes a placement frame 601, a control motor 602 is fixedly installed on the upper end of the placement frame 601, a turntable 603 is fixedly installed on the output end of the control motor 602, a number of positioning racks 6031 for placing petri dishes are provided on the surface of the turntable 603, a receiving box 604 is fixedly installed on the lower side of the turntable 603, and a flow guide groove 6032 is opened inside the turntable 603, which is connected to the receiving box 604.

[0036] The positioning frame 6031 can be used to store culture dishes, and the drain pipe 405 can be used to drain stem cells and transfer them into culture dishes, realizing automatic receiving operation. Subsequently, the position of the positioning frame 6031 can be switched by starting the control motor 602 to realize automatic receiving of multiple culture dishes. The guide channel 6032 can receive cleaning liquid and transfer it into the receiving box 604, so that the cleaning liquid is centrally processed after cleaning, reducing manual operation.

[0037] The working principle of this type of stem cell culture separation device: In use, first open the flip cover 4 and put the storage tube 9 containing the stored stem cells into the corresponding placement seat 8042. Then close the flip cover 4 and start the drive motor 502 to make the mounting plate 803 rotate, thereby centrifuging the storage tube 9 stored in the placement seat 8042, causing the storage tube 9 to separate into layers, thus achieving the separation of stem cells. After separation is completed, the position of the liquid collection needle 406 inside the storage tube 9 is determined by the optical sensor transmitter 11 and the optical sensor receiver 12, and the height of the storage tube 9 is adjusted by the electric push rod 503 so that the liquid collection needle 406 is located at the stem cell layer. Then, the drive motor 4041 is started, which drives the extrusion wheel 4092 to continuously extrude the delivery hose 410, causing negative pressure to be generated inside the liquid extraction needle 406 to extract the stem cells and discharge them from the drain pipe 405. After extraction and separation are completed, the first solenoid valve 4032 is opened and the drive motor 4041 rotates in both directions. Under the action of the squeeze wheel 4092, the delivery hose 410 is squeezed to draw out the cleaning liquid and clean the delivery hose 410, the drain pipe 405 and the liquid collection needle 406. This is beneficial for the extraction of stem cells in the next storage test tube 9 and avoids the stem cells from being contaminated. After the stem cell extraction and separation of a single storage tube 9 is completed, the drive motor 502 is used to switch to other storage tubes 9 to achieve continuous extraction.

[0038] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. For those skilled in the art, other variations or modifications can be made based on the above description. It is impossible to exhaustively list all the implementation methods here. All obvious variations or modifications derived from the technical solutions of the present invention are still within the protection scope of the present invention.

Claims

1. A separation device for stem cell culture, comprising a base (1), wherein a separator (2) is fixedly mounted on the upper side of the base (1), characterized in that: The separator (2) is equipped with a centrifugation assembly (8) for separating stem cells. The base (1) is equipped with a drive assembly (5) in the middle. The separator (2) is equipped with an auxiliary assembly (6) for storing culture dishes on one side. The base (1) is equipped with a PLC controller (7) on one side. The base (1) is equipped with a support frame (3) on the other side. The support frame (3) is equipped with a slide rail (301). The slide rail (301) is equipped with a flip seat (401) inside. The flip seat (401) is equipped with a flip cover (4) at one end. The flip cover (4) is equipped with a sealing ring (402) on the outside. The flip cover (4) is located at the upper end of the separator (2).

2. The separation device for stem cell culture according to claim 1, characterized in that: The flip cover (4) has a rotating groove (408) on one side. A rotating frame (409) is rotatably installed inside the rotating groove (408). A transfer groove (4091) is provided at the end of the rotating frame (409). A squeezing wheel (4092) is provided inside the transfer groove (4091). Exchange channels (411) are provided at the upper and lower ends of the rotating groove (408). A sealing seat (407) is provided at the end of each exchange channel (411). A delivery hose (410) is installed between two sealing seats (407). A drain pipe (405) is fixedly installed at the upper sealing seat (407). A liquid-taking needle (406) is fixedly installed at the lower sealing seat (407). The two ends of the delivery hose (410) are connected to the drain pipe (405) and the liquid-taking needle (406) respectively.

3. The separation device for stem cell culture according to claim 2, characterized in that: A mounting groove (404) is provided on one side of the rotating groove (408). A drive motor (4041) is fixedly installed inside the mounting groove (404). The output end of the drive motor (4041) extends to the rotating groove (408) and is fixedly connected to the middle of the rotating frame (409). The upper surface of the flip cover (4) is equipped with a liquid storage box (403), and the drain end of the liquid storage box (403) is provided with an extension pipe (4031). The extension pipe (4031) is connected to the body of the drain pipe (405), and the body of the extension pipe (4031) is equipped with a first solenoid valve (4032).

4. The separation device for stem cell culture according to claim 1, characterized in that: The drive assembly (5) includes a drive motor (502), which is fixedly installed inside one side of the base (1). The partition cylinder (2) has several transmission slots (5022) inside. One of the transmission slots (5022) has a drive gear (5021) rotatably installed inside, and the other two transmission slots (5022) have mating gears (5023) rotatably installed inside. The output end of the drive motor (502) is fixedly connected to the drive gear (5021).

5. The separation device for stem cell culture according to claim 4, characterized in that: A boss (501) is fixedly installed on one side of the base (1). An electric actuator (503) and a hydraulic chamber (5032) are fixedly installed on the upper end of the boss (501). A piston rod (5031) is fixedly installed at the output end of the electric actuator (503). The piston rod (5031) is located inside the hydraulic chamber (5032). A transfer pipe (5034) is installed at one end of the hydraulic chamber (5032). A second solenoid valve (5033) is installed on the body of the transfer pipe (5034).

6. The separation device for stem cell culture according to claim 5, characterized in that: The centrifugal assembly (8) includes a fixed cylinder (801), which is fixedly installed at the center of the separator cylinder (2). A rotating sleeve (802) is rotatably mounted inside the fixed cylinder (801) via a rotating bearing (8011). A driven gear (8013) is provided on the outside of the rotating sleeve (802), which meshes with the driving gear (5021) and the mating gear (5023). The rotating sleeve (802) has an opening inside. A hydraulic groove (8021) is provided inside which a splined shaft (8022) is movably and sealed. A piston is provided at the bottom of the splined shaft (8022). A mounting plate (803) is provided at the upper end of the splined shaft (8022). Several extension brackets (804) are installed on the outside of the mounting plate (803). A rotating seat (8041) is provided at the outer end of the extension bracket (804). A placement seat (8042) is rotatably installed inside each rotating seat (8041).

7. The separation device for stem cell culture according to claim 6, characterized in that: The upper end of the adapter pipe (5034) extends to the bottom of the hydraulic groove (8021) and rotates to engage with it. A flat bearing (8012) is installed between the bottom of the rotating sleeve (802) and the body of the adapter pipe (5034). A sealed bearing is provided between the body of the adapter pipe (5034) and the hydraulic groove (8021).

8. The separation device for stem cell culture according to claim 6, characterized in that: The placement base (8042) has light-transmitting grooves (8043) at both ends. A column (10) is fixedly installed on one side of the bottom of the partition cylinder (2). An optical sensor receiver (12) is fixedly installed on one side of the column (10). An optical sensor transmitter (11) is fixedly installed on one side of the inner wall of the partition cylinder (2). The optical sensor transmitter (11) and the optical sensor receiver (12) are electrically connected to the PLC controller (7). The storage test tube (9) is placed inside the placement seat (8042). The upper end of the storage test tube (9) is equipped with a top cover (901). A sealing gasket (902) is provided on the lower side of the top cover (901). A perforation (903) is opened in the middle of the top cover (901).

9. The separation device for stem cell culture according to claim 8, characterized in that: The column (10) has an open movable groove (1001) inside. A movable rod (1003) is movably installed inside the movable groove (1001) by a support spring (1002). A limit frame (1004) is installed at one end of the movable rod (1003).

10. The separation device for stem cell culture according to claim 1, characterized in that: The auxiliary component (6) includes a mounting frame (601), a control motor (602) is fixedly installed on the upper end of the mounting frame (601), a turntable (603) is fixedly installed on the output end of the control motor (602), a plurality of positioning racks (6031) for placing petri dishes are provided on the surface of the turntable (603), a receiving box (604) is fixedly installed on the lower side of the turntable (603), and a flow guide groove (6032) is opened inside the turntable (603), which is connected to the receiving box (604).

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