Laser assisted transparent tape perforator

Abstract

The utility model provides a kind of laser-assisted transparent zone punching device, belong to medical instrument technical field.The device includes image acquisition module, analysis processing module, laser emission module, control guide module and laser guide module, image acquisition module is used to carry out image shooting to cell in culture dish, image acquisition module is connected with analysis processing module signal, laser emission module is set on control guide module, control guide module is used to control the emission angle and position of laser emission module, control guide module is connected with analysis processing module signal, laser emission module is irradiated on the cell of culture dish by laser guide module.The utility model embodiment provided a kind of laser-assisted transparent zone punching device, can solve the problem of lower accuracy and safety in prior art.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to a laser-assisted transparent strip perforation device. Background Technology

[0002] With the development of embryo culture technology, existing techniques include using lasers to ablate the zona pellucida, thereby effectively improving the embryo implantation rate and shortening the time for patients to achieve successful conception.

[0003] In existing technologies, embryo perforation or thinning procedures mainly rely on manual operation based on the operator's experience, which presents several problems. Firstly, the manual assessment of embryonic development using parameters such as zona pellucida thickness is highly subjective, with significant differences in judgment between different operators, leading to a lack of accuracy and consistency in the selection of perforation location, size, and timing. Secondly, manual operation makes it difficult to precisely control perforation parameters, easily causing damage to the embryo and affecting its subsequent development.

[0004] Existing embryo drilling devices are usually operated manually, which results in low accuracy and safety of the embryo drilling operation. Utility Model Content

[0005] This utility model provides a laser-assisted transparent strip drilling device, which can solve the problems of low accuracy and safety in the prior art. The technical solution is as follows:

[0006] A laser-assisted zona pellucida perforation device for performing operations such as perforation and thinning of cells in culture dishes includes: an image acquisition module, an analysis and processing module, a laser emission module, a control and guidance module, and a laser guidance module.

[0007] The image acquisition module is used to capture images of the cells in the culture dish. The image acquisition module is signal-connected to the analysis and processing module. The laser emission module is mounted on the control and guidance module. The control and guidance module is used to control the emission angle and position of the laser emission module. The control and guidance module is signal-connected to the analysis and processing module. The laser emission module irradiates the cells in the culture dish with laser light through the laser guidance module.

[0008] Optionally, the laser emitting module includes an infrared ablation laser source, an indicator laser source, and an optical fiber coupler. The optical fiber coupler is disposed at the output end of the infrared ablation laser source and the indicator laser source, and is used to integrate and output the laser emitted by the infrared ablation laser source and the indicator laser source.

[0009] Optionally, the laser emitting module further includes an optical fiber collimator disposed between the optical fiber coupler and the laser guiding module.

[0010] Optionally, the control guidance module includes a pulley and a motor, the laser emission module is mounted on the pulley, the motor is used to control the rotation of the pulley, and the motor is signal-connected to the analysis and processing module.

[0011] Optionally, the laser guiding module includes a concave reflector and a dichroic mirror. The concave reflector is disposed at the output end of the laser emitting module and is angled to the laser. The dichroic mirror is disposed at the output end of the concave reflector and is connected to the culture dish.

[0012] Optionally, the laser guiding module further includes an objective lens, which is disposed at the output end of the dichroic mirror and connected to the culture dish.

[0013] Optionally, the laser guiding module further includes a first compound eye lens and a second compound eye lens, which are arranged in opposite directions between the concave reflector and the dichroic mirror.

[0014] Optionally, the image acquisition module is an industrial camera.

[0015] Optionally, the analysis and processing module is a high-performance image processing unit.

[0016] The beneficial effects of the technical solution provided by this utility model embodiment include at least the following:

[0017] This invention provides a laser-assisted zona pellucida perforation device. An image acquisition module captures images of cells in a culture dish and transmits signals to an analysis and processing module. The analysis and processing module uses its internal algorithm to analyze and process the images, extracting the contour of the zona pellucida, calculating its thickness, determining whether perforation is necessary, and identifying the optimal perforation location and size. Then, it transmits signals to a control and guidance module, controlling the laser emission module to emit laser light at the correct position and angle. The laser light is then directed onto the cells in the culture dish via the laser guidance module. This structure enables precise laser perforation of cells in the culture dish, effectively addressing the issues of low accuracy and safety in existing technologies. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the overall structure provided in an embodiment of the present utility model;

[0020] Figure 2 This is a front view schematic diagram of the structure and operation of the laser emitting module and the control and guidance module provided in this embodiment of the utility model;

[0021] Figure 3 This is a schematic diagram of signal transmission provided in an embodiment of the present utility model;

[0022] Figure 4 This is a diagram showing the changes in optical path transmission provided in an embodiment of this utility model.

[0023] In the diagram: 101-Cultural dish; 1-Image acquisition module; 2-Analysis and processing module; 3-Laser emission module; 31-Infrared ablation laser source; 32-Infrared ablation laser source; 33-Fiber optic coupler; 34-Fiber optic collimator; 4-Control and guidance module; 41-Pulley; 42-Motor; 5-Laser guidance module; 51-Concave mirror; 52-Dichroic mirror; 53-Objective lens; 54-First compound eye lens; 55-Second compound eye lens. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of this utility model clearer, the embodiments of this utility model will be described in further detail below with reference to the accompanying drawings.

[0025] Figure 1 This is a schematic diagram of the overall structure provided in an embodiment of the present utility model; Figure 2 This is a front view schematic diagram of the structure and operation of the laser emitting module and the control and guidance module provided in this embodiment of the utility model; Figure 3 This is a schematic diagram of signal transmission provided in an embodiment of the present utility model; Figure 4 This is a diagram showing the optical path transmission changes provided in an embodiment of this utility model. For example... Figures 1 to 4The device shown is a laser-assisted zona pellucida perforation device used for perforating and thinning cells in a culture dish 101. It includes: an image acquisition module 1, an analysis and processing module 2, a laser emission module 3, a control and guidance module 4, and a laser guidance module 5. The image acquisition module 1 is used to capture images of the cells in the culture dish 101. The image acquisition module 1 is signal-connected to the analysis and processing module 2. The laser emission module 3 is mounted on the control and guidance module 4. The control and guidance module 4 is used to control the emission angle and position of the laser emission module 3. The control and guidance module 4 is signal-connected to the analysis and processing module 2. The laser emission module 3 irradiates the cells in the culture dish 101 with laser light through the laser guidance module 5.

[0026] For example, in this embodiment of the invention, an image acquisition module 1 acquires images of cells in a culture dish and transmits signals to an analysis and processing module 2. The analysis and processing module 2 uses its internal algorithm to analyze and process the images, extracting the contour of the zona pellucida, calculating its thickness, determining whether drilling is necessary, and identifying the optimal drilling position and size. Then, it transmits signals to a control and guidance module 4 to control the laser emission module 3 to emit laser light at its position and angle. The laser light is then directed onto the cells in the culture dish via a laser guidance module 5. This structure enables precise drilling of cells in the culture dish 101, effectively addressing the issues of low accuracy and safety in existing technologies. While existing laser-assisted drilling devices improve drilling accuracy to some extent, they lack intelligent analysis of the embryo's condition and cannot adaptively adjust the drilling strategy based on the specific embryo's situation. With the rapid development of artificial intelligence technology, applying it to embryo drilling equipment to achieve precise embryo analysis and intelligent drilling operations has significant practical implications.

[0027] Optionally, the laser emitting module 3 includes an infrared ablation laser source 31, an indicator laser source 32, and an optical fiber coupler 33. The optical fiber coupler 33 is disposed at the output end of the infrared ablation laser source 31 and the indicator laser source 32, and is used to integrate the laser emitted by the infrared ablation laser source 31 and the indicator laser source 32 and output it.

[0028] For example, in this embodiment of the present invention, the laser wavelength emitted by the indicator laser source 32 is 650nm, and the laser wavelength emitted by the infrared ablation laser source 31 is 1480nm. The infrared ablation laser source 31 is used to punch holes in embryonic cells, but since it is invisible to the naked eye, the indicator laser source 32 and the infrared ablation laser source 31 need to be integrated through the fiber optic coupler 33 so that the integrated laser beam not only has the function of punching holes but also has visibility. The integrated laser beam is then guided by the laser guiding module 5.

[0029] Optionally, the laser emitting module 3 also includes an optical fiber collimator 34, which is disposed between the optical fiber coupler 33 and the laser guiding module 5.

[0030] For example, in this embodiment of the present invention, by setting the fiber collimator 34, the laser beam integrated by the fiber coupler 33 can be collimated to form a parallel beam, which is beneficial for the laser to propagate in space, thereby further improving the accuracy of the device.

[0031] Optionally, the control guidance module 4 includes a pulley 41 and a motor 42. The laser emission module 3 is mounted on the pulley 41, and the motor 42 is used to control the rotation of the pulley 41. The motor 42 is connected to the analysis and processing module 2 via signal.

[0032] Exemplary, in embodiments of this utility model, such as Figure 1 and 2 As shown, Figure 1 This is a top view diagram showing the structural arrangement of the laser emission module and the control and guidance module. Figure 2 This is a front view schematic diagram of the structure and operation of the laser emitting module and the control and guidance module. The laser emitting module 3 is mounted on a pulley 41. By controlling the rotation of the motor 42, the pulley 41 rotates, causing the laser emitting module 3 to move laterally in two dimensions. This allows the laser emitted by the laser emitting module 3 to irradiate the laser guidance module 5 at different positions and angles, thereby adjusting the final position of the laser irradiation on the embryonic cells and improving the flexibility of the device. This embodiment only presents one form of the control and guidance module 4 for moving the laser emitting module 3. The control and guidance module 4 can also be constructed using gear and rack transmission, etc. Various movements that allow the laser emitting module 3 to translate can serve as structural forms for the control and guidance module 4.

[0033] Optionally, the laser guiding module 5 includes a concave reflector 51 and a dichroic mirror 52. The concave reflector 51 is disposed at the output end of the laser emitting module 3 and is set at an angle to the laser. The dichroic mirror 52 is disposed at the output end of the concave reflector 51 and the output end of the dichroic mirror 52 is connected to the culture dish 101.

[0034] For example, in this embodiment of the invention, a basic optical path for laser transmission is formed by setting a concave reflector 51 and a dichroic mirror 52. The concave reflector 51 is arranged at an angle relative to the light emission direction of the fiber optic collimator 34 to guide the light beam emitted by the laser emitting module 3 to irradiate the culture dish 101. The laser guiding module 5 can be a unit integrated into a microscope in the laboratory, and can itself be used as part of the microscope when the zona pellucida of the embryo is not being perforated.

[0035] Optionally, the laser guiding module 5 also includes an objective lens 53, which is located at the output end of the dichroic mirror 52 and is connected to the culture dish 101.

[0036] For example, in this embodiment of the present invention, by setting the objective lens 53, the light beam output from the output end of the dichroic mirror 52 can be focused, thereby making the laser beam irradiating the culture dish 101 more concentrated, thereby further improving the accuracy of the device.

[0037] Optionally, the laser guiding module 5 also includes a first compound eye lens 54 and a second compound eye lens 55, which are arranged in opposite directions between the concave reflector 51 and the dichroic mirror 52.

[0038] For example, in this embodiment of the present invention, after the point light source is emitted from the laser emitting module 3, it becomes a parallel beam after passing through the fiber collimator 34. Then, after the concave reflector 51 changes the direction of the light path, it illuminates the first compound eye lens 54. After passing through the first compound eye lens 54, the parallel light is split into multiple beams. The second compound eye lens 55 is located at the focal plane of the first compound eye lens 54, and the first compound eye lens 54 and the second compound eye lens 55 are arranged back-to-back and opposite to each other. After passing through the second compound eye lens 55, the multiple beams are reflected by the dichroic mirror and reach the objective lens 53. After passing through the objective lens 53, a uniform light spot is formed, thus realizing the shaping of the Gaussian beam into a flat-top beam. Figure 4As shown in the figure, the concave reflector 51 and dichroic mirror 52, which change the direction of the light path, are omitted, thus illustrating the transformation process from the point light source to the objective lens 53. Existing technologies generally use Gaussian beams (the native output beam of a laser, with a bell-shaped intensity distribution that is high in the center and low at the edges) for processing, which has the following significant drawbacks: First, uneven energy distribution leads to poor processing morphology; second, there is a significant risk of thermal damage, with an excessively large heat-affected zone. The extremely high energy density at the center of the Gaussian beam generates a large amount of instantaneous heat, causing heat to diffuse to the surrounding area of ​​the zona pellucida and adjacent oocytes / embryo cytoplasm, forming a large heat-affected zone. Excessive thermal effects may damage the structural integrity of the zona pellucida, induce heat shock in oocytes / embryos, and even affect the subsequent embryonic developmental potential; third, the drilling accuracy and repeatability are poor. The drilling size or the size / depth of the thinning area is extremely sensitive to fluctuations in laser pulse energy, making it difficult to achieve high-precision, high-repeatability processing. In this embodiment, by setting a first compound eye lens 54 and a second compound eye lens 55, the Gaussian beam emitted from the laser emitting module 3 is converted into a flat-top beam. This overcomes the technical defects of existing methods for drilling the zona pellucida using Gaussian beams, providing a laser processing system and method for embryonic zona pellucida with high precision, minimal thermal damage, and superior drilling effect. Its core lies in using the flat-top beam converted by the first compound eye lens 54 and the second compound eye lens 55 as the ablation source. By utilizing the uniform energy distribution and steep edge characteristics of the flat-top beam, the core problems existing in the processing of embryonic zona pellucida with Gaussian beams are fundamentally solved.

[0039] Optionally, the image acquisition module 1 is an industrial camera.

[0040] For example, in this embodiment of the invention, the industrial camera has a compact structure, supports 24-hour continuous operation, and can operate stably in harsh environments such as high temperatures, exhibiting high stability and adaptability. The industrial camera's shutter speed can be as short as microseconds, and its frame rate can reach hundreds or even thousands of frames per second. Employing progressive scan technology, the industrial camera achieves higher image quality.

[0041] Optionally, the analysis and processing module 2 is a high-performance image processing unit.

[0042] Exemplarily, in this embodiment of the invention, the analysis and processing module 2 is the core component of the device, comprising both hardware and software. The hardware employs a high-performance graphics processing unit (GPU) server to meet the AI ​​algorithm's need for rapid processing of large amounts of data. On the software side, deep learning algorithms are used to analyze the acquired embryonic images. Through learning and training on a large amount of embryonic image data, the AI ​​model can accurately identify the zona pellucida thickness, the embryonic developmental stage (such as cleavage-stage embryos, blastocysts, etc.), and the internal structural features of the embryo (such as the inner cell mass, trophoblast cells, etc.). For example, a semantic segmentation algorithm based on convolutional neural networks (CNN) is used to perform pixel-level segmentation of the embryonic images, accurately extracting the contour of the zona pellucida, and then calculating the zona pellucida thickness. Simultaneously, based on the embryo's morphological characteristics and developmental stage, combined with a decision-making model established using medical expert experience and a large amount of experimental data, the AI ​​analysis and processing system can automatically determine whether the embryo needs to be perforated and determine the optimal perforation location and size. Then, by controlling the movement of the guide module 4, the fully automatic laser-assisted perforation operation is completed. Alternatively, an AI analysis and processing system can quickly analyze embryo images and provide perforation suggestions. Operators can then quickly perform the operation based on the suggestions, which greatly improves operational efficiency and shortens operation time compared to traditional manual judgment and operation methods.

[0043] Unless otherwise defined, the technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains. The terms “first,” “second,” and similar terms used in this patent application specification and claims do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Similarly, the terms “an” or “a” and similar terms do not indicate a quantity limitation, but rather indicate the presence of at least one. The terms “comprising” or “including” and similar terms mean that the elements or objects preceding “comprising” or “including” encompass the elements or objects listed following “comprising” or “including” and their equivalents, and do not exclude other elements or objects. The terms “connected” or “linked” and similar terms are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. The terms “upper,” “lower,” “left,” and “right” are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0044] The above description is only an optional embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A laser-assisted zona pellucida perforation device for performing operations such as perforation and thinning of cells in a culture dish (101), characterized in that, include: Image acquisition module (1), analysis and processing module (2), laser emission module (3), control and guidance module (4) and laser guidance module (5). The image acquisition module (1) is used to capture images of the cells in the culture dish (101). The image acquisition module (1) is connected to the analysis and processing module (2). The laser emission module (3) is mounted on the control and guidance module (4). The control and guidance module (4) is used to control the emission angle and position of the laser emission module (3). The control and guidance module (4) is connected to the analysis and processing module (2). The laser emission module (3) irradiates the cells in the culture dish (101) with laser light through the laser guidance module (5).

2. The laser-assisted transparent strip punching device according to claim 1, characterized in that, The laser emitting module (3) includes an infrared ablation laser source (31), an indicator laser source (32), and an optical fiber coupler (33). The optical fiber coupler (33) is disposed at the output end of the infrared ablation laser source (31) and the indicator laser source (32) to integrate the laser emitted by the infrared ablation laser source (31) and the indicator laser source (32) and output it.

3. The laser-assisted transparent strip punching device according to claim 2, characterized in that, The laser emitting module (3) further includes an optical fiber collimator (34), which is disposed between the optical fiber coupler (33) and the laser guiding module (5).

4. The laser-assisted transparent strip punching device according to claim 1, characterized in that, The control and guidance module (4) includes a pulley (41) and a motor (42). The laser emission module (3) is mounted on the pulley (41). The motor (42) is used to control the rotation of the pulley (41). The motor (42) is connected to the analysis and processing module (2) via signal.

5. The laser-assisted transparent strip punching device according to claim 1, characterized in that, The laser guiding module (5) includes a concave reflector (51) and a dichroic mirror (52). The concave reflector (51) is disposed at the output end of the laser emitting module (3) and is set at an angle to the laser. The dichroic mirror (52) is disposed at the output end of the concave reflector (51) and the output end of the dichroic mirror (52) is connected to the culture dish (101).

6. The laser-assisted transparent strip punching device according to claim 5, characterized in that, The laser guiding module (5) also includes an objective lens (53), which is disposed at the output end of the dichroic mirror (52) and the output end of the objective lens (53) is connected to the culture dish (101).

7. The laser-assisted transparent strip punching device according to claim 6, characterized in that, The laser guiding module (5) further includes a first compound eye lens (54) and a second compound eye lens (55), which are arranged in opposite directions between the concave reflector (51) and the dichroic mirror (52).

8. The laser-assisted transparent strip punching device according to claim 1, characterized in that, The image acquisition module (1) is an industrial camera.

9. A laser-assisted transparent strip punching device according to claim 1, characterized in that, The analysis and processing module (2) is a high-performance image processing unit.

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