A method for determining quaternary ammonium salt pesticides in blood by liquid chromatography mass spectrometry

Abstract

The application discloses a method for determining quaternary ammonium salt pesticides in blood by using liquid chromatography mass spectrometry, which draws a standard working curve of whole blood (plasma, serum) matrix by liquid chromatography mass spectrometry analysis, obtains peak areas of target quantitative ion peaks and peak areas of internal standard quantitative ion peaks of different concentrations of paraquat, diquat, wild wheat, chlormequat, and mepiquat, and then draws a standard working curve; and under the same conditions, liquid chromatography mass spectrometry analysis is carried out on whole blood sample liquid, peak areas of target quantitative ion peaks and peak areas of internal standard quantitative ion peaks in the whole blood sample liquid are obtained at the same time, and the content of each quaternary ammonium salt pesticide is calculated according to the standard working curve. The application can simply, quickly and simultaneously detect the content of five kinds of quaternary ammonium salt pesticides in whole blood of poisoned patients, and has the characteristics of simplicity, accuracy, good repeatability and high sensitivity.

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