Chrysanthemum indicum cutting rooting method

Abstract

The invention relates to the technical field of agriculture and forestry, and discloses a chrysanthemum indicum cutting rooting method. The invention provides a chrysanthemum indicum cutting rooting method. The chrysanthemum indicum cutting rooting method comprises the following steps: S1, soaking cutting slips subjected to primary cutting in a mixed aqueous solution of a rooting agent and a bactericide, and then inserting the cutting slips into a hole tray filled with a cutting medium for cutting seedling raising; s2, spraying water during cutting seedling raising, transplanting 15 days after cutting, or performing topping to obtain terminal buds 25 days after cutting and / or after cutting seedlings are higher than 20cm, and transplanting the topped cutting seedlings; the terminal buds obtained through topping serve as cutting slips for secondary cutting, and the step S1 is repeated for secondary cutting seedling raising. According to the cutting rooting method, rapid propagation of wild chrysanthemum flowers can be achieved, obtained cutting seedlings have the good rooting rate and root length, the quality of new seedlings is guaranteed, secondary cutting can be continued, the propagation coefficient of mother plants of the wild chrysanthemum flowers is increased, the time for obtaining cutting slips is shortened, and the cost for obtaining the cutting slips is saved.

Description

Technical Field

[0001] This invention relates to the field of agricultural and forestry technology, specifically to a method for rooting wild chrysanthemum cuttings. Background Technology

[0002] Wild chrysanthemum is a plant of the Asteraceae family called wild chrysanthemum. Chrysanthemum indicum The dried capitulum of *L.* is harvested in autumn and winter when the flowers first open, then sun-dried or steamed and sun-dried. It has the effects of clearing heat and detoxifying, purging fire and calming the liver, and is used for boils, carbuncles, red and swollen eyes, headaches, and dizziness. Wild chrysanthemum, also known as bitter chrysanthemum or roadside yellow chrysanthemum, is one of the main raw materials for cold and flu granules. Wild chrysanthemum is a perennial herb that grows on grassy slopes, in thickets, wetlands along rivers, coastal saline areas, field edges, and roadsides. With the continuous growth of the cold medicine market, the demand for wild chrysanthemum is also increasing. However, due to the decreasing wild resources of wild chrysanthemum and the fact that production mainly relies on seed propagation, seedlings show significant differences between individuals, making it difficult to guarantee excellent quality. Furthermore, existing planting methods are time-consuming, inefficient, and costly, making it difficult to ensure a sustainable supply of wild chrysanthemum raw materials. Therefore, there is an urgent need to develop a method that can meet production needs while ensuring quality and enabling the industrialized cultivation of wild chrysanthemum. Summary of the Invention

[0003] This invention provides a method for rooting wild chrysanthemum cuttings to solve the problems of long cycle, low efficiency, high cost and inability to guarantee the quality of wild chrysanthemums when propagating them in large quantities using existing technologies.

[0004] In a first aspect, the present invention provides a method for rooting wild chrysanthemum cuttings, comprising the following steps: Step S1: Soak the cuttings for primary propagation in a mixed aqueous solution of rooting agent and fungicide, and then insert them into a seedling tray containing propagation substrate for propagation; the primary cuttings include the terminal buds of healthy plants; the rooting agent includes α-naphthaleneacetic acid and indolebutyric acid; the fungicide includes carbendazim and / or chlorothalonil; Step S2: Spray water during the cutting propagation period. Transplant 15 days after cutting, or 25 days after cutting and / or when the cuttings are taller than 20cm, pinch off the top to obtain the terminal bud, and transplant the topped cuttings. The terminal bud obtained by pinching off the top is used as a cutting for secondary cutting and Step S1 is repeated for secondary cutting propagation.

[0005] Fifteen days after cutting propagation, the roots of the cuttings are covered with substrate and ready for transplanting. Transplanting should be completed within one month of the start of propagation, before the roots mature. After 25 days, or when the cuttings reach a height of 20cm, the tops of the cuttings can be pinched off, retaining the terminal bud, and the same method of propagation can be continued. The seedlings should be transplanted promptly after pinching off the tops.

[0006] The cuttings for the first planting are taken using a cutting board or a fixed-length iron sheet.

[0007] Using suspended seedbed frames prevents the roots of cuttings from growing into the soil, promotes substrate drainage, and ensures uniform and stable substrate moisture. Excessive moisture can lead to mold growth in the substrate.

[0008] The entire cutting process involves shading, proper ventilation, and maintaining a relative balance of humidity and temperature.

[0009] After the cuttings begin, control the moisture in the seedbed by spraying water so that water droplets fall from the bottom of the seedling trays. After one week, when the roots of the cuttings have grown to about 1 cm, start to reduce the moisture in the seedbed and keep the substrate moist.

[0010] In one optional embodiment, the length of the cutting is 5-6 cm; In one optional embodiment, the cuttings are stored at 2-4℃; the cuttings are made by cutting a fixed length of leaves from the terminal bud without any treatment, and can be uniformly packaged in a breathable container and placed in a cold storage at 4℃ for extended storage of 4-5 days.

[0011] In one optional embodiment, the α-naphthaleneacetic acid has a mass concentration of 0.005%-0.05% in the mixed aqueous solution.

[0012] In one optional embodiment, the indolebutyric acid has a mass concentration of 0.005%-0.05% in the mixed aqueous solution.

[0013] In one optional embodiment, the carbendazim concentration in the mixed aqueous solution is 0.03%-0.05% by mass.

[0014] In one optional embodiment, the mass concentration of chlorothalonil in the mixed aqueous solution is 0.1%-0.2%.

[0015] In one optional embodiment, the soaking time is 0.5-1 hour.

[0016] In one optional embodiment, the scion is inserted to a depth of 2-4 cm, just enough to keep the scion fixed and prevent it from falling over.

[0017] In one alternative embodiment, the cutting substrate includes peat moss, perlite, and vermiculite; the peat moss substrate is beneficial for maintaining stable moisture and aeration at the base of the cuttings.

[0018] In one alternative embodiment, the volume ratio of peat, perlite, and vermiculite is 4-6:2-4:1-2.

[0019] In one alternative embodiment, the acupuncture plate is a 72-well acupuncture plate.

[0020] In one alternative embodiment, the size of the acupuncture plate is 540×280mm.

[0021] In one optional embodiment, the seedling trays are placed on a suspended seedbed frame. The suspended seedbed frame prevents the roots of the cuttings from growing into the soil, promotes drainage, and ensures stable substrate moisture and aeration. Excessive moisture will affect the substrate and promote mold growth.

[0022] In one optional embodiment, the spraying time is controlled in segments: when the length of the cutting is less than or equal to 1 cm, spraying is carried out for 60-90 minutes every 24 hours; when the length of the cutting is greater than or equal to 1 cm, spraying is carried out for 30-60 minutes every 24 hours.

[0023] In one alternative implementation, the step of topping is further included 15 days after the cuttings are propagated and before transplanting.

[0024] In one optional implementation, the method of jacking is mechanical jacking.

[0025] In one alternative implementation, the transplanting is completed within 30 days after the start of cutting propagation.

[0026] In one alternative implementation, the topped cuttings are hardened off before transplanting; two days before leaving the nursery, the cuttings are topped and the substrate is thoroughly watered to harden off.

[0027] The technical solution of this invention has the following advantages: 1. The present invention provides a method for rooting wild chrysanthemum cuttings, comprising the following steps: Step S1: Soaking the cuttings from the first cutting in a mixed aqueous solution of a rooting agent and a fungicide, and then inserting them into a seedling tray containing a cutting substrate for propagation; the first cutting includes the terminal bud of a healthy plant; the rooting agent includes α-naphthaleneacetic acid and indolebutyric acid; the fungicide includes carbendazim and / or chlorothalonil; Step S2: Spraying with water during the propagation period, transplanting 15 days after cutting, or, 25 days after cutting and / or when the cuttings are taller than 20cm, pinching off the terminal bud, and transplanting the pinched cuttings; the terminal bud obtained is used as a cutting for the second cutting, and Step S1 is repeated for the second propagation. The cutting rooting method provided by this invention can inherit the excellent properties of the wild chrysanthemum mother plant and quickly propagate one superior plant into many small plants. It boasts high propagation efficiency, strong operability, and enables rapid propagation of wild chrysanthemums. The cuttings obtained using this method exhibit good rooting rates and root lengths, ensuring the quality of the new seedlings. The selection of the cutting site is particularly important; the use of terminal buds in this invention ensures rapid rooting and the formation of new seedlings. After 25 days of cutting or when the cuttings are taller than 20cm, the top is removed, retaining the terminal bud for secondary cuttings. This increases the propagation coefficient of the wild chrysanthemum mother plant, shortens the time for obtaining cuttings, and saves on cutting costs. This method is efficient, controllable, and easily facilitates the industrialized production of wild chrysanthemums.

[0028] 2. This invention provides a method for rooting wild chrysanthemum cuttings. The cuttings are taken using a cutting board or a fixed-length iron sheet to ensure uniform height during propagation. Placing the cuttings in breathable packaging at 2-4℃ extends their storage time by 4-5 days, facilitating mass production. The choice of substrate and the concentration of the rooting solution promote rooting and seedling establishment. Maintaining consistent spraying frequency and timing also benefits mass propagation. Using suspended seedbed trays during seedling cultivation prevents roots from penetrating the soil, promotes substrate drainage, and allows for reuse of the trays, while also reducing the incidence of pests and diseases.

[0029] 3. The present invention provides a method for rooting wild chrysanthemum cuttings, which involves topping the cuttings 15 days after they are taken out of the nursery and before transplanting them, thus reducing the steps in agricultural production and saving agricultural production costs. Attached Figure Description

[0030] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0031] Figure 1 This is a representative image of the cuttings after 15 days of propagation in Embodiment 1 of the present invention; Figure 2 This is a representative image of a 20cm tall cutting seedling in Embodiment 1 of the present invention; Figure 3 These are representative figures showing the rooting of cuttings from Examples 1-4 and Comparative Examples 2-5 in Experiment 1 of this invention. Detailed Implementation

[0032] The following embodiments are provided to better understand the present invention, but the following embodiments do not constitute a limitation on the content and scope of protection of the present invention. Any product that is the same as or similar to the present invention, derived by any person under the guidance of the present invention or by combining the features of the present invention with other prior art, falls within the scope of protection of the present invention.

[0033] Unless otherwise specified, all experimental steps or conditions in the examples were performed according to conventional experimental procedures and conditions in the art. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0034] The materials and reagents used in the various embodiments and comparative examples of this invention are as follows: Wild chrysanthemum mother plants required for cuttings were selected from the wild chrysanthemum germplasm resource nursery jointly established by Huangshi CR Sanjiu Pharmaceutical Co., Ltd. and its partners. The selected plants were strong and free from pests and diseases.

[0035] Cuttings: Use a cutting board to cut 5-6cm of the terminal buds or middle and lower branches of the test mother plant as propagation material. The cuttings do not need to be treated. After being packaged in breathable packaging, they can be stored in a cold storage at 4℃ for 4-5 days.

[0036] The water used in the rooting process of cuttings was all groundwater.

[0037] Rooting agent: Named Genpan, purchased from Sichuan Guoguang Agrochemical Co., Ltd., specification: 200mL / bottle, formulation: soluble concentrate, content of α-naphthaleneacetic acid (NAA) and indolebutyric acid (IBA) is 2.5% each, and the total active ingredient content is 5%.

[0038] Carbendazim: Purchased from Sichuan Runer Technology Co., Ltd., dosage form: wettable powder, specification: 400g / bag, of which carbendazim content is 50%.

[0039] Imported peat substrate: purchased from Sanyi (Shouguang) Agricultural Co., Ltd. The substrate consists of peat soil, perlite, and vermiculite in a volume ratio of 5:3:2. The size is 7-22mm and the specification is 100L / bag.

[0040] Example 1 This embodiment provides a method for rooting wild chrysanthemum cuttings, including the following steps: (1) 100 terminal buds from the mother plant were used as cuttings. All cuttings were soaked in a mixed aqueous solution with mass concentrations of 0.005% NAA, 0.005% IBA and 0.05% carbendazim for 1 hour. (2) Insert the soaked cuttings into a seedling tray filled with imported peat substrate, ensuring that the bottom of the cutting is in the hole and the cutting is secure. The insertion depth is 2-4 cm. (3) Keep the seedbed moist during the seedling period, provide shade throughout the process, and spray with water. Spray for 60 minutes every 12 hours. At this time, water droplets will fall from the bottom of the seedling tray. After one week, when the roots of the cuttings grow to about 1 cm, start to further control the moisture in the seedbed. Spray for 30 minutes every 12 hours to keep the substrate moist. (4) After 15 days of seedling cultivation (e.g.) Figure 1 As shown), the root system of the cuttings has been covered with substrate and can be mechanically topped before being transplanted. Transplanting should be completed within one month of the start of propagation, when the root system is not yet mature. Alternatively, it can be done after 25 days or when the cuttings are taller than 20cm (e.g., ...). Figure 2As shown), top the cuttings and retain the terminal bud to continue cutting from step (1). After topping, the cuttings should be transplanted in time, and hardening off should be done before transplanting.

[0041] Example 2 The method for rooting wild chrysanthemum cuttings provided in this embodiment is basically the same as that in embodiment 1, except for the mass concentration of the effective components in the soaking solution of the cuttings. The soaking solution used in this embodiment is a mixed aqueous solution with mass concentrations of 0.01% NAA, 0.01% IBA and 0.03% carbendazim.

[0042] Example 3 The method for rooting wild chrysanthemum cuttings provided in this embodiment is basically the same as that in embodiment 1, except that the only difference is the mass concentration of the effective ingredients in the soaking solution of the cuttings. The soaking solution used in this embodiment is a mixed aqueous solution with mass concentrations of 0.025% NAA, 0.025% IBA and 0.04% carbendazim.

[0043] Example 4 The method for rooting wild chrysanthemum cuttings provided in this embodiment is basically the same as that in embodiment 1, except for the mass concentration of the effective ingredients in the soaking solution of the cuttings. The soaking solution used in this embodiment is a mixed aqueous solution with mass concentrations of 0.05% NAA, 0.05% IBA and 0.2% chlorothalonil.

[0044] Comparative Example 1 The method for rooting wild chrysanthemum cuttings provided in this comparative example is basically the same as that in Example 1, except that the source part of the cuttings is the terminal bud, and the soaking solution used for the cuttings contains only a medium concentration of carbendazim as in Example 1, without the addition of NAA and IBA.

[0045] Comparative Example 2 The method for rooting wild chrysanthemum cuttings provided in this comparative example is basically the same as that in Example 1, except that the cuttings are sourced from the middle and lower branches.

[0046] Comparative Example 3 The method for rooting wild chrysanthemum cuttings provided in this comparative example is basically the same as that in Example 2, except that the cuttings are sourced from the middle and lower branches.

[0047] Comparative Example 4 The method for rooting wild chrysanthemum cuttings provided in this comparative example is basically the same as that in Example 3, except that the cuttings are sourced from the middle and lower branches.

[0048] Comparative Example 5 The method for rooting wild chrysanthemum cuttings provided in this comparative example is basically the same as that in Example 4, except that the cuttings are sourced from the middle and lower branches.

[0049] Comparative Example 6 The method for rooting wild chrysanthemum cuttings provided in this comparative example is basically the same as that in Comparative Example 1, except that the cuttings are sourced from the middle and lower branches.

[0050] Comparative Example 7 The method for rooting wild chrysanthemum cuttings provided in this comparative example is basically the same as that in Example 1, except that the rooting agent (NAA and IBA compound) in the soaking solution used for the cuttings is replaced with a 0.01% naphthaleneacetic acid aqueous solution.

[0051] Comparative Example 8 The method for rooting wild chrysanthemum cuttings provided in this comparative example is basically the same as that in Example 1, except that the rooting agent (NAA and IBA compound) in the soaking solution used for the cuttings is replaced with an indolebutyric acid aqueous solution with a mass concentration of 0.01%.

[0052] Experimental Example 1 This experimental example provides the seedling cultivation conditions for each embodiment and comparative example, as detailed below: Each example and comparative example used 100 cuttings, which were propagated in seed trays. After 15 days of seedling cultivation, the substrate was gently rinsed off with water without damaging the roots. The number of roots per cutting was counted, and the average number of roots per rooted plant was calculated. The root length of each root was measured, and the average root length of the rooted plant was calculated. Results are shown below. Figure 3 See Table 1.

[0053] Table 1 Rooting Status

[0054] from Figure 3 As shown in Table 1, the method for rooting wild chrysanthemum cuttings provided by this invention can ensure a high rooting rate for cuttings, resulting in a large average number of roots and a long root length in the seedlings. A comparison between Comparative Example 1 and Example 1 shows that the rooting solution used in this invention significantly promotes rooting at the base of the cuttings. Compared to the examples, Comparative Examples 2-6 show that the location of the cuttings affects the rooting rate. Although lower and middle branches can also be used as cuttings, terminal buds are easier to obtain, resulting in faster rooting, upright growth, and easier mass propagation and management. The seedlings are also less prone to mold and disease. Comparative Examples 7 and 8 used single rooting agents. Since naphthaleneacetic acid (NAA) mainly promotes root elongation and reduces lateral roots, while indolebutyric acid (IBA) mainly promotes adventitious and lateral roots, using either alone cannot simultaneously increase the number and length of roots, so the effect is not as good as the examples. This invention, by controlling the selection of cuttings and the seedling cultivation process, can ensure that the new seedlings have a good rooting rate and root length.

[0055] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A method for rooting cuttings of Chrysanthemum indicum, characterized in that, The method comprises the following steps: S1: soaking the first cutting seedling in a mixed water solution of rooting agent and fungicide, and then inserting the seedling into a plug tray filled with cutting substrate; the first cutting seedling comprises the terminal bud of a healthy plant; the rooting agent comprises α-naphthaleneacetic acid and indole butyric acid; the fungicide comprises carbendazim and / or chlorothalonil; S2: spraying water during the cutting cultivation, transplanting after 15 days of cutting, or cutting for 25 days and / or cutting seedling height being higher than 20 cm, and then topping to obtain the terminal bud, and transplanting the seedling after topping; the terminal bud obtained by topping is used as the second cutting seedling to repeat the S1 step for secondary cutting cultivation.

2. The method for rooting of wild chrysanthemum cuttings according to claim 1, characterized by, The length of the first cutting seedling is 5-6 cm; And / or, the seedling is stored at 2-4 ℃.

3. The method for rooting of chrysanthemum morifolium cuttings according to claim 1, wherein, The mass concentration of the α-naphthaleneacetic acid in the mixed water solution is 0.005%-0.05%; And / or, the mass concentration of the indole butyric acid in the mixed water solution is 0.005%-0.05%; And / or, the mass concentration of the carbendazim in the mixed water solution is 0.03%-0.05%; And / or, the mass concentration of the chlorothalonil in the mixed water solution is 0.1%-0.2%.

4. The method for rooting of chrysanthemum morifolium cuttings according to claim 1, wherein, The soaking time is 0.5-1 h.

5. The method for rooting of chrysanthemum morifolium cuttings according to claim 1, wherein, The insertion depth of the seedling is 2-4 cm.

6. The method for rooting of chrysanthemum morifolium cuttings according to claim 1, wherein, The cutting substrate comprises peat soil, perlite and vermiculite; Optionally, the volume ratio of the peat soil, perlite and vermiculite is 4-6:2-4:1-2.

7. The method for rooting of chrysanthemum morifolium cuttings according to claim 1, wherein, The plug tray is a 72-hole plug tray; And / or, the size of the plug tray is 540×280 mm.

8. The method of rooting cuttings of wild chrysanthemum according to claim 1, wherein, The plug tray is placed on a suspended seedling bed frame.

9. The method for rooting of chrysanthemum morifolium cuttings according to claim 1, wherein, The spraying time is controlled in stages, and when the length of the seedling is less than or equal to 1 cm, spraying is performed for 60-90 min every 24 h, and when the length of the seedling is greater than or equal to 1 cm, spraying is performed for 30-60 min every 24 h.

10. The method for rooting of chrysanthemum morifolium cuttings according to claim 1, wherein, The cutting cultivation further comprises the step of topping after 15 days of cutting and before transplanting; And / or, the topping is mechanical topping; And / or, the transplanting is completed within 30 days after the start of the cutting cultivation; And / or, the seedling after topping is hardened before transplanting.

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