Pesticide composition for preventing and controlling virus diseases of economic crops and application of pesticide composition

Abstract

The invention discloses a pesticide composition for preventing and controlling viral diseases of economic crops and application of the pesticide composition. The pesticide composition comprises streptomyces albidosus CK15, streptomyces roseoflavus NZK-259 and moroxydine hydrochloride cupric acetate. According to the invention, streptomyces albidoflavus CK15, streptomyces roseoflavus NZK-259 and moroxydine hydrochloride cupric acetate are combined for use, so that the composition has the advantages of a microbial pesticide and a chemical pesticide, can promote plant growth, improve crop stress resistance, induce crops to generate disease resistance, has an excellent inhibition effect on viruses, and has a good application prospect. The three components have a synergistic effect, so that the disease resistance and the virus killing capacity of crops are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pesticides, in particular to a pesticide composition for preventing and controlling viral diseases of economic crops. BACKGROUND

[0002] Viral diseases are called "plant cancer", which has caused serious economic losses to agricultural production, especially economic crops, and has become one of the most difficult plant diseases to prevent and control. At present, the officially registered pesticide varieties for preventing and controlling viral diseases in China include toxic fluorophosphorus, amino oligosaccharide, lentinan, salt of xincunamine, morpholinium hydrochloride, and sterol, etc. Biological pesticides and chemical pesticides are combined, and the former is mainly used. After viral infection of plant tissues, the effect of chemical or biological pesticides is limited, but the development of chemical pesticides combined with biological pesticides for prevention and control is less seen in current production practice.

[0003] Morpholinium hydrochloride and copper acetate are a registered product for preventing and controlling viral diseases, which is mainly 20% wettable powder. Morpholinium hydrochloride is a broad-spectrum, low-toxicity viral control agent, which enters the plant body through the water stomata after spraying on the leaves of crops, inhibits or destroys the formation of nucleic acid and lipoprotein, and prevents the replication process of viruses, thereby playing a role in preventing and controlling viral diseases. Copper acetate mainly prevents or controls other diseases caused by fungi through copper ions, thereby playing an auxiliary role. The combination of the two has a synergistic effect and is better than single agent, but because the effect is not outstanding, the current market application is not much.

[0004] Rosea streptomyces (NKZ-259, accession number: CGMCC NO. 13416) belongs to aerobic, gram-positive actinomycetes, and is an excellent biocontrol strain. It has strong secondary metabolism, and can produce a large amount of secondary metabolites during its growth process, which has a good effect on improving crop yield and quality. Streptomyces albus (CK15, accession number: CGMCC NO. 31251) belongs to actinomycetes, and has the characteristics of low toxicity, high efficiency, broad spectrum, and environmental protection. The valin produced by CK-15 and other secondary metabolites in the fermentation process can effectively inhibit or kill a variety of pathogenic bacteria harmful to plants, and is mainly used for preventing and controlling fungal diseases of crops, and has a significant effect on powdery mildew, gray mold, and apple scab.

[0005] CK-15 is rarely reported for preventing and controlling viral diseases. At present, only Huang Kecheng reported that CK-15 combined with NKZ259 has a good effect on preventing and controlling viral diseases of melon (2025, Changjiang Vegetables, Field Evaluation of Two Immune Inducers on Viral Diseases of Melon and Pepper), and can promote the growth of pepper and improve the yield of pepper.

[0006] There is no report on the combination of CK-15, NKZ-259, and morpholinium hydrochloride·copper acetate for preventing and controlling viral diseases. SUMMARY

[0007] The purpose of this invention is to address the shortcomings of the prior art by providing a pesticide composition for the prevention and control of viral diseases in economic crops.

[0008] To achieve the above objectives, the present invention adopts the following technical solution: The pesticide composition includes CK-15, NZK-259, and morpholine guanidine hydrochloride·copper acetate.

[0009] Furthermore, CK-15 is Strepfomyces albulus, with accession number CGMCC NO. 31251, accession date of July 10, 2024, and the strain is deposited at the China General Microbiological Culture Collection Center.

[0010] Furthermore, NKZ-259 is Streptomyces roseaflavus, with accession number CGMCC NO. 13416, accession date December 2, 2016, and the strain is deposited at the China General Microbiological Culture Collection Center.

[0011] Preferably, as a preferred embodiment, the mass fraction of CK-15 and NKZ-259 is 0.5-5%, preferably 1%; Preferably, as a preferred embodiment, the mass fraction of morpholine guanidine hydrochloride·copper acetate is 20%, wherein the mass ratio of morpholine guanidine hydrochloride to copper acetate is 1:1.

[0012] Preferably, as a preferred embodiment, the morpholine guanidine hydrochloride·copper acetate is a 20% morpholine guanidine hydrochloride·copper acetate wettable powder.

[0013] Preferably, as a preferred embodiment, the weight ratio of CK15, NZK-259, and morpholine guanidine hydrochloride·copper acetate is 1.5:1.5:1; Another object of the present invention is to provide the application of the above-mentioned pesticide composition in the prevention and control of viral diseases of economic crops, preferably, the economic crops are chili peppers and tobacco; Preferably, as a preferred embodiment, the viral disease is an RNA virus disease, including but not limited to mosaic virus disease, potato Y virus disease, vein spot virus disease, ring spot virus disease, and tomato wilt virus disease.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention combines CK-15, NZK-259, and morpholine guanidine hydrochloride·copper acetate, which has a significant synergistic effect on viral diseases. It has a significant preventive effect on viral diseases, can induce disease resistance in crops, promote crop growth and development, and increase crop yield, resulting in significant economic benefits in actual production. Detailed Implementation

[0015] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0016] As described in the background section, viral diseases are known as "plant cancers," causing serious economic losses to agricultural production, especially to cash crops, and becoming one of the most difficult plant diseases to control.

[0017] Based on this, the present invention conducts in-depth research on CK-15, NZK-259, and morpholine guanidine hydrochloride·copper acetate. Combining the above products can effectively promote the growth and development of peppers and tobacco, increase crop yield, and at the same time improve the prevention effect against viral diseases of peppers and tobacco, thus having a synergistic effect.

[0018] I. Preparation Examples The pesticide products used in the embodiments of this invention are all commercially available products. CK-15 used in these embodiments was obtained from the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, with accession number CGMCC NO. 31251. CK-15 was inoculated into YEME medium and cultured on a shaker at 28°C for 2 days, then inoculated into fermentation medium and cultured on a shaker at 28°C for another 64 hours. The fermentation broth was then filtered, and the supernatant obtained was the CK-15 fermentation broth. The YEME medium formula was: 10g glucose, 3g malt extract, 3g yeast powder, 5g tryptone, 340g sucrose, and distilled water to a final volume of 1000mL. Sterilization was performed at 121°C for 30 minutes. The fermentation broth culture medium formula was: 2g soybean meal, 0.4g (NH4)2SO4, 2g glucose, 3g corn flour, 0.3g CaCO3, and distilled water to a final volume of 1000mL. Sterilization was performed at 121°C for 30 minutes. The fermentation broth of CK-15 obtained from fermentation was filtered through a 0.22 μm microporous filter to obtain CK-15AS.

[0019] The NZK-259 used in this embodiment of the invention was obtained from the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, with the preservation number CGMCC NO. 13416. NKZ-259 was inoculated onto Gao's No. 1 medium and cultured at 28℃ for 7 days. Then, 1cm pieces of the bacteria were cut with a scalpel and inoculated into fermentation medium, followed by shaking fermentation for 96 h (28℃, 220 rpm). The fermentation broth was then centrifuged (10000 r / min, 4℃), and the supernatant was collected and filtered through a 0.22μm filter membrane to obtain NKZ-259AS. The formula of Gao's No. 1 medium (g / L) is: soluble starch 20.0 g, potassium nitrate 1.0 g, dipotassium hydrogen phosphate 0.5 g, magnesium sulfate heptahydrate 0.5 g, sodium chloride 0.5 g, ferrous sulfate heptahydrate 0.01 g, and agar 17 g. After weighing, the contents were dissolved in distilled water by heating and stirring, dispensed into Erlenmeyer flasks, autoclaved at 121℃, and then plated.

[0020] The 20% morpholine guanidine hydrochloride·copper acetate WP used in this embodiment of the invention was obtained from Dongguan Ruidefeng Biotechnology Co., Ltd.; the 30% fluorophos WP was purchased from Guangxi Tianyuan Biochemical Co., Ltd. through market channels.

[0021] II. Bioassay Examples 1. Example 1: The control and growth-promoting effects of CK-15 + NKZ-259 + 20% morpholine guanidine hydrochloride·copper acetate WP on pepper virus disease. The experiment was conducted in a field in Zengcheng, Guangzhou, with a total of 6 treatments. The treatments and pesticide dosages are shown in Table 1. Each plot was 100 m². 2 Four replicates were set up. Ten days after transplanting, before the onset of viral disease, foliar spraying was initiated using a 3WBD-20 backpack electric sprayer (Xuzhou Lanyi Plant Protection Equipment Co., Ltd.). Spraying was repeated every 7 days for a total of three applications. Viral disease index and control efficacy were assessed 7 days after each application. The results are shown in Table 1. Viral disease began to appear in the field after the third application. During the experiment, production management, including pesticide application and fertilization, remained consistent across all treatments. Crown width, flower number, and plant height were measured 7 days after the third application. Pepper yield was measured in each plot on the day of harvest and converted to a yield equivalent to 667 m². 2 Yields, results are shown in Table 2.

[0022] Table 1. Control efficacy of CK-15 + NKZ-259 + morpholine guanidine hydrochloride·copper acetate combination against pepper viral diseases.

[0023] Note: All products are prepared by double dilution; the same applies below. Table 2. Effects of the CK-15 + NKZ-259 + Morpholine Guanidine Hydrochloride·Copper Acetate Regimen on Pepper Growth Promotion

[0024] As shown in Table 1, after three applications, the combination of CK-15 + NKZ-259 + morpholine guanidine hydrochloride and copper acetate showed the highest efficacy against pepper virus disease, reaching 91.68%, which was significantly better than the control treatments CK-15 + NKZ-259 + cyfluthrin, CK-15 + NKZ-259, and morpholine guanidine hydrochloride and copper acetate alone. This indicates that the combination of CK-15, NKZ-259, and morpholine guanidine hydrochloride and copper acetate has a good synergistic effect against pepper virus disease.

[0025] As shown in Table 2, after three applications, the CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate treatment group showed better results than the control treatments CK-15+NKZ-259+flufenoxam, CK-15+NKZ-259, and morpholine guanidine hydrochloride·copper acetate alone. This indicates that the combined application of CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate can effectively promote chili growth and increase its yield.

[0026] 2. Example 2: Control and growth-promoting effects of CK-15 + NKZ-259 + morpholine guanidine hydrochloride·copper acetate on pepper virus disease. The experiment was conducted in a field in Leshan, Sichuan Province, with a total of 6 treatments. The treatments and pesticide dosages are shown in Table 3. Each plot was 80 m². 2 Four replicates were set up. Foliar spraying was initiated 8 days after transplanting, before viral disease appeared, using a 3WBD-20 backpack electric sprayer (Sichuan Yizhi Intelligent Equipment Co., Ltd.). Spraying was repeated every 7 days for a total of 3 applications. Viral disease index and control efficacy were assessed 7 days after each application. Results are shown in Table 3. Viral disease began to appear in the field after the third application. Production management, including pesticide application and fertilization, remained consistent across all treatments during the experiment. Crown width, flower number, and plant height were measured 7 days after the third application. Pepper yield was measured in each plot on the day of harvest and converted to a yield equivalent to 667m². 2 Yields, results are shown in Table 4.

[0027] Table 3. Efficacy of the CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate regimen in the control of pepper virus disease.

[0028] Table 4. Effects of the CK-15 + NKZ-259 + Morpholine Guanidine Hydrochloride·Copper Acetate Regimen on Pepper Growth Promotion

[0029] As shown in Table 3, after three applications, the combination of CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate showed the highest control efficacy against pepper virus disease, reaching 91.22%, which was significantly better than the control treatments CK-15+NKZ-259+flufenoxam, CK-15+NKZ-259, and morpholine guanidine hydrochloride·copper acetate alone. This indicates that the combination of CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate has a good control effect against pepper virus disease, and the combination of morpholine guanidine hydrochloride·copper acetate and CK-15+NKZ-259 has a good synergistic effect in the control of pepper virus disease.

[0030] As shown in Table 4, after three applications, the CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate treatment group showed better results than the control treatments CK-15+NKZ-259+flufenoxam, CK-15+NKZ-259, and morpholine guanidine hydrochloride·copper acetate alone. This indicates that the combined application of CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate can effectively promote chili growth and increase its yield.

[0031] 3. Example 3: Effect of CK-15 + NKZ-259 + Morpholine Guanidine Hydrochloride·Copper Acetate on the Prevention and Control of Tobacco Virus Diseases The experiment was conducted in a field in Wenshan, Yunnan Province, with a total of 6 treatments. The treatments and pesticide dosages are shown in Table 5. Each plot was 150 m². 2 Four replicates were set up. Foliar spraying was initiated 10 days after transplanting, before viral disease occurred, using a 3WBD-20 backpack electric sprayer (Zhejiang Chaonongli Intelligent Technology Co., Ltd.). Spraying was repeated every 7 days thereafter, for a total of 4 applications. Viral disease index and control efficacy were assessed 7 days after each application. The results are shown in Table 5. Viral disease began to appear in the field after the fourth application. During the experiment, production management practices, including pesticide application and fertilization, remained consistent across all treatments.

[0032] Table 5. Efficacy of the CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate regimen in the prevention and control of tobacco viral diseases.

[0033] As shown in Table 5, after four applications, the combination of CK-15 + NKZ-259 + morpholine guanidine hydrochloride and copper acetate showed the highest efficacy against tobacco viral disease, reaching 80.99%, which was significantly better than the control treatments CK-15 + NKZ-259 + cyfluthrin, CK-15 + NKZ-259, and morpholine guanidine hydrochloride and copper acetate alone. This indicates that the combination of CK-15 + NKZ-259 + morpholine guanidine hydrochloride and copper acetate has a good control effect on tobacco viral disease, and the combination of morpholine guanidine hydrochloride and copper acetate with CK-15 + NKZ-259 has a good synergistic effect on tobacco viral disease.

[0034] 4. Example 4: The effect of CK-15 + NKZ-259 + morpholine guanidine hydrochloride·copper acetate on the prevention and control of tobacco virus diseases and its growth-promoting effect. The experiment was conducted in a field in Chuxiong, Yunnan Province, with a total of 6 treatments. The treatments and pesticide dosages are shown in Table 6. Each plot was 120 m². 2 Four replicates were set up. Ten days after transplanting, before the onset of viral disease, foliar spraying was initiated using a 3WBD-20 backpack electric sprayer (Ningbo Muzhi Tools Co., Ltd.). Spraying was then performed every 7-10 days for a total of four applications. Viral disease index and control efficacy were assessed seven days after each application; results are shown in Table 6. Viral disease began to appear in the field after the fourth application. Production management practices, including pesticide application and fertilization, remained consistent across all treatments during the experiment. Plant height, crown width, and leaf area were measured seven days after the fourth application; results are shown in Table 7.

[0035] Table 6. Efficacy of the CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate regimen in the prevention and control of tobacco viral diseases.

[0036] Table 7. Effects of the CK-15 + NKZ-259 + Morpholine Guanidine Hydrochloride·Copper Acetate Regimen on Tobacco Growth Promotion

[0037] As shown in Table 6, after four applications, the combination of CK-15 + NKZ-259 + morpholine guanidine hydrochloride and copper acetate showed the highest efficacy against tobacco viral diseases, reaching 83.80%, which was significantly better than the control treatments CK-15 + NKZ-259 + cyfluthrin, CK-15 + NKZ-259, and morpholine guanidine hydrochloride and copper acetate alone. This indicates that the combination of CK-15 + NKZ-259 + morpholine guanidine hydrochloride and copper acetate has a good control effect on tobacco viral diseases, and the combination of morpholine guanidine hydrochloride and copper acetate with CK-15 + NKZ-259 has a good synergistic effect in the control of tobacco viral diseases.

[0038] As shown in Table 7, after four applications, the tobacco plant height, crown width, and leaf area of ​​the CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate treatment group were all better than those of the control treatments CK-15+NKZ-259+flufenoxam, CK-15+NKZ-259, and morpholine guanidine hydrochloride·copper acetate alone. This indicates that the combined use of CK-15+NKZ-259+morpholine guanidine hydrochloride·copper acetate can effectively promote tobacco growth.

[0039] The foregoing has provided a detailed description of one embodiment of the present invention, but this description is merely a preferred embodiment and should not be construed as limiting the scope of the invention. All equivalent variations and modifications made within the scope of the claims of this invention should still fall within the patent coverage of this invention.

Claims

1. A pesticide composition for the prevention and control of viral diseases of economic crops, characterized by, The pesticide composition comprises CK-15, NZK-259, morpholinium hydrochloride and copper acetate; wherein, The CK-15 is Streptomyces albus, and the preservation number is CGMCC NO. 31251; The NKZ-259 is Streptomyces roseus, and the preservation number is CGMCC NO. 13416; The mass fraction of the CK-15 and the NKZ-259 is 0.5-5%; The mass fraction of the morpholinium hydrochloride and copper acetate is 20%.

2. The pesticidal composition for preventing and controlling viral diseases of economic crops according to claim 1, characterized by, The mass ratio of the morpholinium hydrochloride and copper acetate is 1:

1.

3. The pesticidal composition for preventing and controlling viral diseases of economic crops according to claim 1, characterized by, The field use dosage of the CK-15, the NZK-259, and the morpholinium hydrochloride and copper acetate is 1.5:1.5:

1.

4. The agricultural pesticide composition for preventing and controlling viral diseases of economic crops according to claim 1, characterized by, The mass fraction of the CK-15 and the NKZ-259 is 1%.

5. The pesticidal composition for preventing and controlling viral diseases of economic crops according to claim 1, characterized by, The morpholinium hydrochloride and copper acetate is 20% morpholinium hydrochloride and copper acetate wettable powder.

6. The pesticide composition for preventing and controlling viral diseases of economic crops according to claim 1 is applied to preventing and controlling viral diseases of economic crops.

7. Use according to claim 6, characterized in that, The economic crops are peppers and tobaccos.

8. Use according to claim 6, characterized in that, The viral diseases are RNA viral diseases, including but not limited to, mosaic viral diseases, potato virus Y viral diseases, vein banding viral diseases, ring spot viral diseases, and tomato spotted wilt viral diseases.

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