The invention relates to a Dufulin-containing ultra low volume liquid formulation, which is prepared by using Dufulin or Dufulin and an active component II as active components and adding an auxiliary agent and a solvent. The active component II is any one selected from the group consisting of ningnanmycin, moroxydine hydrochloride, amino-oligosaccharin, chlorobromoisocyanuric acid and lentinan. The invention has advantages of simple processing, high work efficiency, water conservation, long persistent period, targets which are not easy to generate drug resistance, low environmental pollution, synergist effects and the like. Through ultra low volume spray, low volume spray or ultra low volume static electro-spray, a plurality of diseases of crops such as paddy rice, tobacco, corn, cotton, orange, capsicum, tomato, rape and the like can be prevented and cured.