Cooperative culture device and method for oil reservoir in-situ surfactant-producing flora and oil-displacing agent

Abstract

The invention belongs to the technical field of microbial oil recovery, and relates to an oil reservoir in-situ surfactant-producing flora and oil-displacing agent collaborative culture device and an oil reservoir in-situ surfactant-producing flora and oil-displacing agent collaborative culture method. Comprising a fermentation tank, the fermentation tank is provided with a PLC, the top of the fermentation tank is provided with a monitoring display unit, an oil-displacing agent quantity-controlled adding assembly and a rotary-drive stirring gas supply assembly, and the monitoring display unit, the oil-displacing agent quantity-controlled adding assembly and the rotary-drive stirring gas supply assembly are all communicated with the interior of the fermentation tank and are all electrically connected with the PLC; a temperature control heating assembly electrically connected with the PLC is arranged on the side wall of the fermentation tank body in a surrounding manner. Through cooperation of the fermentation tank, the PLC, the rotary drive stirring gas supply assembly and the oil displacement agent quantity control adding assembly, stirring and uniform mixing can be conducted in the fermentation tank, so that the liquid concentration is consistent, the dissolved oxygen value is monitored in real time, gas injection and oxygenation are automatically conducted in the fermentation tank when the dissolved oxygen value is lower than a preset value, and a favorable environment is provided for fermentation cooperative culture work; the culture effect reduction caused by the low dissolved oxygen value is avoided, and the collaborative culture effect is improved.

Description

Technical Field

[0001] This invention belongs to the field of microbial enhanced oil recovery technology, and relates to a device and method for co-cultivating surfactant-producing bacteria and oil displacement agents in situ in oil reservoirs. Background Technology

[0002] With the continuous advancement of the biological field, an increasing number of microbial strains are being widely applied in industries such as biopharmaceuticals and oil extraction. For example, in oil extraction applications, reservoir microbial resources are utilized to activate and promote the growth and reproduction of oil-producing functional bacteria through activating nutrients, thereby achieving the goal of enhancing oil recovery. Modified nano-oil displacement materials are compounded with microorganisms or microbial metabolites (such as biosurfactants) to form composite bio-based nano-oil displacement materials, thereby achieving the goal of enhancing oil recovery. The synergistic cultivation of microbial inoculants and oil displacement agents aims to improve crude oil recovery through the synergistic effect of microbial fermentation and oil displacement agents (such as biosurfactants and polymers).

[0003] Existing in-situ reservoir surfactant-producing microbial communities and oil displacement agents co-cultivation devices have the following shortcomings: 1. The addition of oil displacement agents relies on manual addition after detecting OD600≥5, making it difficult to automatically control the amount of oil displacement agents added, resulting in unsatisfactory ease of use; 2. Existing methods involve stirring to achieve homogeneous mixing and co-cultivation of microbial solutions and oil displacement agents, but automatic oxygenation is not possible when dissolved oxygen levels are low, inevitably reducing the cultivation effect and the co-cultivation effect. In view of this, this application proposes an in-situ reservoir surfactant-producing microbial communities and oil displacement agents co-cultivation device to solve the above-mentioned problems. Summary of the Invention

[0004] The purpose of this invention is to provide a device and method for co-cultivating surfactant-producing microbial communities and oil displacement agents in situ in oil reservoirs, so as to solve the technical problem that existing oil displacement agents rely on artificial addition and cannot automatically perform oxygenation when the dissolved oxygen value is low, resulting in a reduction in the co-cultivation effect. To achieve the above objectives, the present invention employs the following technical solution: In a first aspect, this application discloses a device for the co-cultivation of surfactant-producing microbial communities and oil displacement agents in situ in oil reservoirs, comprising a fermenter, a PLC controller mounted on the fermenter, a monitoring and display unit, an oil displacement agent dosage control addition component, and a rotary agitator and gas supply component mounted on the top of the fermenter, all of which are connected to the interior of the fermenter and electrically connected to the PLC controller; a temperature control and heating component is arranged around the side wall of the fermenter, and the temperature control and heating component is electrically connected to the PLC controller.

[0005] Preferably, the fermenter has four legs fixedly connected at equal intervals at the bottom, and a cover plate fixedly installed on the top. The monitoring and display unit, the oil displacement agent dosage control and addition component, and the rotary stirring and gas supply component are all fixed on the cover plate. The cover plate is also provided with a feeding pipe and an exhaust pipe that are connected to the inside of the fermenter, and a discharge valve at the end of the fermenter.

[0006] Preferably, the monitoring and display unit includes a display, a surfactant concentration sensor, and a monitoring sensor. The display, surfactant concentration sensor, and monitoring sensor are all electrically connected to the PLC controller. The display is located above the PLC controller. The monitoring sensor is located at the top of the fermenter 1, with its detection end extending into the fermenter. The surfactant concentration sensor is located on the side wall of the fermenter, with its detection end extending into the fermenter.

[0007] Preferably, the monitoring sensors include a pH sensor, a DO sensor, and a biosensor.

[0008] Preferably, the oil displacement agent controlled addition component includes an annular box, which is set at the top of the fermentation tank for storing the oil displacement agent. A liquid addition pipe is set at the bottom of the annular box, and a liquid flow sensor is set on the liquid addition pipe. A solenoid valve is fixedly connected to the bottom of the liquid flow sensor, and the bottom of the solenoid valve extends into the fermentation tank. Both the solenoid valve and the liquid flow sensor are electrically connected to the PLC controller. The rotary agitator gas supply component is fixed at the top of the annular box and extends into the fermentation tank.

[0009] Preferably, the rotary agitator gas supply assembly includes a fixed box, which is fixed to the top of the oil displacement agent control addition assembly. A rotating tube is rotatably fitted into the bottom of the fixed box and extends through the oil displacement agent control addition assembly into the fermentation tank. Several agitator tubes, all located inside the fermentation tank, are fixed to the outside of the rotating tube. Both the agitator tube and the rotating tube are hollow tubular structures, and their interiors are connected. Several air jet holes are opened on the agitator tube, and air inlets are opened on the top of the four sides of the rotating tube, located inside the fixed box. A drive motor with an output shaft fixedly connected to the top of the rotating tube is fixedly installed on the top of the fixed box. An air pump is installed on one side of the fixed box, and the air outlet of the air pump is connected to the fixed box. Both the drive motor and the air pump are electrically connected to the PLC controller.

[0010] Preferably, the bottom of the fixing box has a circular through hole, and a sealed bearing is fixedly fitted inside the circular through hole. The inner ring of the sealed bearing is fixedly fitted to the outer side of the rotating tube.

[0011] Preferably, the temperature control heating assembly includes an electric heating jacket and a temperature sensor. The electric heating jacket is fitted on the outside of the fermentation tank, and the temperature sensor is installed on the side wall of the fermentation tank. The detection end of the temperature sensor extends into the inside of the fermentation tank. Both the temperature sensor and the electric heating jacket are electrically connected to the PLC controller.

[0012] Secondly, this application discloses a method for the co-cultivation of surfactant-producing microorganisms and oil displacement agents in situ of an oil reservoir, implemented based on the co-cultivation device for surfactant-producing microorganisms and oil displacement agents described in any of the above claims, comprising: Surfactant bacteria and nutrient solution are added to the fermenter. The monitoring and display unit monitors and displays the pH value, dissolved oxygen value, biomass and liquid surfactant concentration in the fermenter, and feeds back the pH value, dissolved oxygen value and biomass to the PLC controller. The temperature control and heating component monitors the temperature in the fermenter and feeds back to the PLC controller in real time. The PLC controller compares the temperature, dissolved oxygen, and biomass with preset thresholds. When the dissolved oxygen is lower than the preset threshold, the rotary agitator gas supply component is activated. When the biomass is not less than the preset threshold, the oil displacement agent dosage control component is activated. When the temperature is lower than the preset threshold, the temperature control heating component is activated for heating. When the dissolved oxygen value reaches the preset threshold, the rotary agitator gas supply component is turned off; when the biomass is less than the preset threshold, the oil displacement agent dosage control component is turned off; when the temperature reaches the preset threshold, the temperature control heating component is turned off.

[0013] Preferably, the rotary agitator gas supply assembly includes: a fixed box, a drive motor, a rotary tube, a stirring tube, and an air pump; the oil displacement agent dosage control assembly includes: an annular housing, a liquid flow sensor, a solenoid valve, and a liquid addition tube; the monitoring and display unit includes a display, a surfactant concentration sensor, and a monitoring sensor; thus, it includes: Surfactant bacteria and nutrient solution are added to the fermenter. Surfactant concentration sensors and monitoring sensors monitor the concentration of liquid surfactant, pH value, dissolved oxygen value and biomass in the fermenter. The display shows the concentration of liquid surfactant, pH value, dissolved oxygen value and biomass in the fermenter in real time. The temperature control heating component monitors the temperature in the fermenter and feeds it back to the PLC controller in real time. The PLC controller compares the temperature, dissolved oxygen value, and biomass with preset thresholds. When the dissolved oxygen value is lower than the preset threshold, the drive motor and air pump are turned on. The drive motor drives the rotating tube and stirring tube, and at the same time, the air pump introduces outside air into the fermenter through the rotating tube and stirring tube to increase oxygen. When the dissolved oxygen value reaches the preset threshold, the drive motor and air pump are turned off. When the biomass is not less than the preset threshold, the solenoid valve is opened; the liquid flow sensor monitors the oil displacement agent entering the fermenter through the liquid addition pipe, and when the flow rate reaches the preset flow rate value, the solenoid valve is closed; after 20 minutes, when the real-time biomass is not less than the preset threshold, the solenoid valve is opened again until the real-time biomass is less than the preset threshold. When the temperature is below the preset threshold, the temperature control heating component is turned on to heat the room; when the temperature reaches the preset threshold, the temperature control heating component is turned off.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. By combining the fermentation tank, PLC controller, rotary agitator and gas supply component and the oil displacement agent dosage control and addition component, the internal mixing of the fermentation tank can be uniform to ensure consistent liquid concentration. 2. Through the combination of the set rotary agitator gas supply component, monitoring sensors and PLC controller, the dissolved oxygen value can be monitored in real time and automatically injected into the fermenter to increase oxygen when it is lower than the preset value, providing a favorable environment for fermentation co-culture, avoiding the reduction of culture effect due to low dissolved oxygen value, and improving the co-culture effect; 3. With the set oil displacement agent dosage control addition component, monitoring sensor and PLC controller, the oil displacement agent in the fermenter can be automatically added in controlled amount according to the biomass to carry out synergistic cultivation, without the need for manual addition, thus improving the degree of automation and convenience of use; 4. By using the temperature control and heating components in conjunction with the fermentation tank, the temperature inside the fermentation tank can be controlled and heated to ensure a good fermentation and culture temperature environment, thereby further improving the culture effect.

[0015] This application incorporates a series of structures that enable uniform mixing within the fermenter, ensuring consistent liquid concentration. It facilitates real-time monitoring of dissolved oxygen levels and automatically injects oxygen into the fermenter when levels fall below a preset value, providing a favorable environment for co-cultivation and preventing reduced cultivation efficiency due to low dissolved oxygen levels. Furthermore, it allows for the automatic, controlled addition of oil-dispatch agents based on biomass, eliminating the need for manual addition and enhancing automation and convenience. Additionally, it enables temperature control within the fermenter, ensuring a suitable fermentation environment. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the device structure according to an embodiment of the present invention; Figure 2 This is a bottom view of the structure according to an embodiment of the present invention; Figure 3 This is a schematic diagram of the front cross-sectional structure according to an embodiment of the present invention.

[0018] The components include: 1. Fermentation tank; 101. Feeding pipe; 2. PLC controller; 3. Display; 301. Surfactant concentration sensor; 302. Monitoring sensor; 4. Electric heating jacket; 401. Temperature sensor; 5. Annular box; 501. Liquid flow sensor; 502. Solenoid valve; 6. Fixing box; 601. Drive motor; 602. Rotary pipe; 603. Stirring pipe; 604. Jet nozzle; 605. Air inlet; 606. Air pump. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0020] Therefore, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.

[0021] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0022] In the description of the embodiments of the present invention, it should be noted that if terms such as "upper," "lower," "horizontal," or "inner" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of the invention is in use, they are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. Furthermore, terms such as "first" and "second" are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0023] Furthermore, the use of the term "horizontal" does not imply that the component must be absolutely horizontal, but rather that it can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0024] In the description of the embodiments of the present invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in the present invention according to the specific circumstances.

[0025] The present invention will now be described in further detail with reference to the accompanying drawings: See Figures 1-3 This application discloses a device for the co-cultivation of surfactant-producing microbial community and oil displacement agent in situ in an oil reservoir. The device is characterized by comprising a fermenter 1, on which a PLC controller 2 is installed. The top of the fermenter 1 is equipped with a monitoring and display unit, an oil displacement agent dosage control addition component, and a rotary agitator and gas supply component. All three components are connected to the interior of the fermenter 1 and are electrically connected to the PLC controller 2. A temperature control and heating component is arranged around the side wall of the fermenter 1 and is electrically connected to the PLC controller 2.

[0026] In some embodiments, a reservoir in-situ surfactant-producing microbial community co-cultivation device with an oil displacement agent includes: Fermentation tank 1 has four support legs fixedly connected in a ring at equal intervals at its bottom, and a cover plate fixedly installed on its top. A feeding pipe 101 connected to the inside of fermentation tank 1 is embedded and fixed on the top left side of the cover plate. A discharge valve is fixedly connected to the bottom of fermentation tank 1. PLC controller 2 is fixedly installed on the top right side of the cover plate; The surfactant concentration sensor 301 is fixedly installed on the right side of the fermenter 1 and electrically connected to the PLC controller 2, and its detection end extends into the fermenter 1. The monitoring sensor 302 consists of three sets, namely a pH sensor, a DO sensor and a biosensor. All three sets of monitoring sensors 302 are fixedly installed on the top of the cover plate, and their detection ends extend into the fermenter 1. All three sets of monitoring sensors 302 are electrically connected to the PLC controller 2. The oil displacement agent dosage control and addition component is fixedly installed on the top of the cover plate and electrically connected to the PLC controller 2; The rotary agitator and air supply assembly is fixedly installed on the top of the oil displacement agent control and addition assembly, and the cover plate is rotatably sleeved on the rotary agitator and air supply assembly. The temperature control heating component is fixedly installed on the outside of the fermentation tank 1 and electrically connected to the PLC controller 2.

[0027] More preferably, an exhaust pipe connected to the inside of the fermenter 1 is embedded and fixed on the top of the cover plate, and a display 3 is fixed and electrically connected to the top of the PLC controller 2.

[0028] In some embodiments, the fermenter 1 is fixedly connected to four legs at equal intervals at the bottom, and a cover plate is fixedly installed on the top. The monitoring and display unit, the oil displacement agent control and addition component, and the rotary stirring and gas supply component are all fixed on the cover plate. The cover plate is also provided with a feeding pipe 101 and an exhaust pipe that are connected to the inside of the fermenter 1, and a discharge valve at the end of the fermenter 1.

[0029] In some embodiments, the monitoring and display unit includes a display 3, a surfactant concentration sensor 301, and a monitoring sensor 302. The display 3, the surfactant concentration sensor 301, and the monitoring sensor 302 are all electrically connected to the PLC controller 2. The display 3 is located above the PLC controller 2. The monitoring sensor 302 is located at the top of the fermenter 1 and its detection end extends into the fermenter 1. The surfactant concentration sensor 301 is located on the side wall of the fermenter 1 and its detection end extends into the fermenter 1.

[0030] In some embodiments, the monitoring sensor 302 includes a pH sensor, a DO sensor, and a biosensor.

[0031] In some embodiments, the oil displacement agent controlled addition component includes an annular box 5, which is disposed at the top of the fermentation tank 1 for storing the oil displacement agent. A liquid addition pipe is disposed at the bottom of the annular box 5, and a liquid flow sensor 501 is disposed on the liquid addition pipe. A solenoid valve 502 is fixedly connected to the bottom of the liquid flow sensor 501. The bottom of the solenoid valve 502 extends into the fermentation tank 1. Both the solenoid valve 502 and the liquid flow sensor 501 are electrically connected to the PLC controller 2. A rotary agitator gas supply component is fixed at the top of the annular box 5 and extends into the fermentation tank 1.

[0032] In some embodiments, the oil displacement agent controlled addition assembly includes an annular box 5 fixedly installed on the top of a cover plate and filled with oil displacement agent. A liquid flow sensor 501 is fixedly connected to the bottom right side of the annular box 5, and a solenoid valve 502 is fixedly connected to the bottom end of the liquid flow sensor 501. The bottom end of the solenoid valve 502 extends into the fermenter 1. Both the solenoid valve 502 and the liquid flow sensor 501 are electrically connected to the PLC controller 2. A liquid addition pipe is fixedly connected to the top right side of the annular box 5.

[0033] In some embodiments, the rotary agitator gas supply assembly includes a fixed box 6, which is fixed to the top of the oil displacement agent control addition assembly. A rotating tube 602 is rotatably fitted into the bottom of the fixed box 6. The rotating tube 602 extends through the oil displacement agent control addition assembly into the fermentation tank 1. Several agitator tubes 603, all located inside the fermentation tank 1, are fixed to the outside of the rotating tube 602. Both the agitator tube 603 and the rotating tube 602 are hollow tubular structures, and their interiors are connected. Several jet holes 604 are opened on the agitator tube 603. Air inlets 605 are opened on the top of the four sides of the rotating tube 602 and are located inside the fixed box 6. A drive motor 601 with an output shaft fixedly connected to the top of the rotating tube 602 is fixedly installed on the top of the fixed box 6. An air pump 606 is installed on one side of the fixed box 6, and the air outlet of the air pump 606 is connected to the fixed box 6. Both the drive motor 601 and the air pump 606 are electrically connected to the PLC controller 2.

[0034] In some embodiments, the rotary agitator gas supply assembly includes a fixed box 6 fixedly installed on the top of the annular housing 5. A rotary tube 602 is rotatably fitted into the bottom of the fixed box 6. The bottom end of the rotary tube 602 extends movably through the annular housing 5 into the fermentation tank 1 and is configured as a sealing structure. A cover plate is rotatably fitted onto the rotary tube 602. Multiple agitator tubes 603, all located in the fermentation tank 1, are connected and fixed to the outside of the rotary tube 602. The end of the agitator tube 603 away from the rotary tube 602 is configured as a sealing structure. Multiple air jet holes 604 are opened on the front and rear sides of the agitator tube 603. Air inlets 605 located in the fixed box 6 are opened on the top of the four sides of the rotary tube 602. A drive motor 601 with an output shaft fixedly connected to the top of the rotary tube 602 is fixedly installed on the top of the fixed box 6. An air pump 606 with an air outlet extending into the left side of the fixed box 6 is fixedly installed. Both the drive motor 601 and the air pump 606 are electrically connected to the PLC controller 2. The bottom of the fixed box 6 has a circular through hole, and a sealed bearing is fixedly fitted inside the circular through hole. The inner ring of the sealed bearing is fixedly fitted to the outer side of the rotating tube 602.

[0035] In some embodiments, the bottom of the fixing box 6 is provided with a circular through hole, and a sealed bearing is fixedly sleeved in the circular through hole. The inner ring of the sealed bearing is fixedly sleeved with the outer side of the rotating tube 602.

[0036] In some embodiments, the temperature control heating assembly includes an electric heating sleeve 4 and a temperature sensor 401. The electric heating sleeve 4 is fitted on the outside of the fermentation tank 1, and the temperature sensor 401 is disposed on the side wall of the fermentation tank 1. The detection end of the temperature sensor 401 extends into the inside of the fermentation tank 1. Both the temperature sensor 401 and the electric heating sleeve 4 are electrically connected to the PLC controller 2.

[0037] In some embodiments, the temperature control heating assembly includes an electric heating sleeve 4 fixedly sleeved on the outside of the fermentation tank 1, and a temperature sensor 401 with its detection end extending into the right side of the fermentation tank 1 is fixedly installed therein. Both the temperature sensor 401 and the electric heating sleeve 4 are electrically connected to the PLC controller 2.

[0038] like Figures 1 to 3 As shown in the figure, this embodiment proposes a device for the co-cultivation of surfactant-producing microbial communities and oil displacement agents in situ within an oil reservoir, comprising: Fermentation tank 1 has four support legs fixedly connected in a ring at equal intervals at its bottom, and a cover plate fixedly installed on its top. A feeding pipe 101 connected to the inside of fermentation tank 1 is embedded and fixed on the top left side of the cover plate. A discharge valve is fixedly connected to the bottom of fermentation tank 1, and an exhaust pipe connected to the inside of fermentation tank 1 is embedded and fixed on the top of the cover plate. The feeding pipe 101 is used for personnel to add surfactant bacteria and nutrient solution to fermentation tank 1. The surfactant bacteria include Pseudomonas, and the nutrient solution includes molasses / crude oil and ammonium nitrate. PLC controller 2 is fixedly installed on the top right side of the cover plate; The surfactant concentration sensor 301 is fixedly installed on the right side of the fermenter 1 and electrically connected to the PLC controller 2, with its detection end extending into the fermenter 1; the surfactant concentration sensor 301 is used to monitor the critical micelle concentration (CMC) of the liquid surfactant in the fermenter 1 and transmit the concentration value to the PLC controller 2. The monitoring sensors 302 consist of three sets: a pH sensor, a DO sensor, and a biosensor. All three sets of sensors 302 are fixedly mounted on the top of the cover plate, with their detection ends extending into the fermenter 1. All three sets of sensors 302 are electrically connected to the PLC controller 2. A display 3 is fixedly mounted on the top of the PLC controller 2 and electrically connected to it. The three sets of sensors 302 are used to monitor the pH value, dissolved oxygen value, and biomass inside the fermenter 1, respectively, and transmit the corresponding values ​​to the PLC controller 2. The display 3 is controlled by the PLC controller 2 to display the monitored pH value, dissolved oxygen value, biomass, and liquid surfactant concentration. The biosensor is an OD600 sensor. The oil displacement agent dosage control addition component is fixedly installed on the top of the cover plate and electrically connected to the PLC controller 2; the oil displacement agent dosage control addition component is used to automatically add oil displacement agent in a controlled manner by the PLC controller 2 when the biomass OD600≥5; The rotary stirring and gas supply assembly is fixedly installed on the top of the oil displacement agent control and addition assembly, and the cover plate is rotated and sleeved on the rotary stirring and gas supply assembly. The rotary stirring and gas supply assembly is used to stir and mix the inside of the fermentation tank 1 under the control of the PLC controller 2, and is also used to extract external gas and inject gas into the fermentation tank 1 to increase oxygen when the dissolved oxygen value is lower than the preset value. The temperature control heating component is fixedly installed on the outside of the fermentation tank 1 and electrically connected to the PLC controller 2; the temperature control heating component is used to control the temperature of the liquid inside the fermentation tank 1 under the control of the PLC controller 2.

[0039] Specifically, the oil displacement agent control addition component includes an annular box 5 fixedly installed on the top of the cover plate and filled with oil displacement agent. Four support rods are fixedly connected at equal intervals in a ring between the bottom of the annular box 5 and the top of the cover plate. A liquid addition pipe is fixedly connected to the top right side of the annular box 5, and a liquid flow sensor 501 is fixedly connected to the bottom right side of the annular box 5. A solenoid valve 502 is fixedly connected to the bottom end of the liquid flow sensor 501. The bottom end of the solenoid valve 502 extends into the fermentation tank 1. Both the solenoid valve 502 and the liquid flow sensor 501 are electrically connected to the PLC controller 2. The annular chamber 5, liquid flow sensor 501, and solenoid valve 502 are configured in conjunction. The PLC controller 2 is pre-set to control the opening of the solenoid valve 502 when the biomass OD600 is greater than or equal to 5, and also pre-sets the flow rate value for controlling the closing of the solenoid valve 502. When the biomass OD600 is greater than or equal to 5, the PLC controller 2 controls the solenoid valve 502 to open. At this time, the oil displacement agent in the annular chamber 5 is added to the fermenter 1 through the liquid flow sensor 501 and the solenoid valve 502 for co-cultivation. The liquid flow sensor 501 detects the flow rate of the oil displacement agent and transmits the flow rate value to the PLC controller 2. When the preset flow rate value is reached, the PLC controller 2 controls the solenoid valve 502 to close, thereby realizing the automatic and controlled addition of oil displacement agent in the fermenter 1.

[0040] Furthermore, the rotary agitator gas supply assembly includes a fixed box 6 fixedly installed on the top of the annular housing 5. A rotary tube 602 is rotatably fitted into the bottom of the fixed box 6. A circular through-hole is provided at the bottom of the fixed box 6, and a sealed bearing is fixedly fitted inside the circular through-hole. The inner ring of the sealed bearing is fixedly fitted to the outer side of the rotary tube 602, achieving a sealed rotational installation of the rotary tube 602. The bottom end of the rotary tube 602 extends movably through the annular housing 5 into the fermenter 1 and is configured as a sealing structure. A cover plate is rotatably fitted onto the rotary tube 602. A circular through-hole is provided at the top of the cover plate, and a first bearing is fixedly fitted inside the circular through-hole. The inner ring of the first bearing is fixedly fitted to the outer side of the rotary tube 602. The rotating tube 602 is rotated and installed. Multiple stirring tubes 603, all located inside the fermentation tank 1, are connected and fixed to the outside of the rotating tube 602. The end of the stirring tube 603 away from the rotating tube 602 is set as a sealing structure. Multiple air jet holes 604 are opened on the front and rear sides of the stirring tube 603. Air inlets 605 are opened on the top of the four sides of the rotating tube 602 and are located inside the fixing box 6. The top of the fixing box 6 is fixedly installed with a drive motor 601 whose output shaft is fixedly connected to the top of the rotating tube 602. The left side of the fixing box 6 is fixedly installed with an air pump 606 whose air outlet extends into it. The drive motor 601 and the air pump 606 are both electrically connected to the PLC controller 2. The set-up fixed box 6, rotating pipe 602, stirring pipe 603, jet nozzle 604, air inlet 605, drive motor 601, and air pump 606 work together. The drive motor 601 drives the rotating pipe 602 to rotate, which in turn drives multiple stirring pipes 603 to rotate, stirring and mixing the contents of the fermentation tank 1 to ensure a uniform liquid concentration. The PLC controller 2 is pre-set to control the dissolved oxygen value at which the air pump 606 starts. When the dissolved oxygen value is lower than the preset value, the PLC controller 2 controls the air pump 606 to start. The air pump 606 draws in external gas and supplies it into the fixed box 6. The gas enters the rotating tube 602 through four air inlets 605, and then flows into multiple stirring tubes 603. It is then supplied into the liquid through multiple jet holes 604. The oxygen contained in the external gas is used to inject oxygen into the liquid. When the dissolved oxygen value reaches the preset value, the PLC controller 2 controls the air pump 606 to automatically shut off, so as to automatically inject oxygen into the fermenter 1 when the dissolved oxygen value is lower than the preset value, thus providing a favorable environment for the fermentation co-cultivation work.

[0041] Furthermore, the temperature control and heating assembly includes an electric heating jacket 4 fixedly sleeved on the outside of the fermenter 1. A temperature sensor 401 with its detection end extending into the fermenter 1 is fixedly installed on the right side of the fermenter 1. Both the temperature sensor 401 and the electric heating jacket 4 are electrically connected to the PLC controller 2. The electric heating jacket 4 and the temperature sensor 401 work together to control the opening and closing temperature value of the electric heating jacket 4 by using the PLC controller 2 to preset the temperature value. The temperature sensor 401 monitors the temperature of the liquid inside the fermenter 1 and transmits the temperature value to the PLC controller 2. The PLC controller 2 controls the electric heating jacket 4 to open and close automatically according to the preset temperature value. The electric heating jacket 4 performs temperature control and heating of the liquid inside the fermenter 1 to ensure a good fermentation and cultivation temperature environment.

[0042] This application also discloses a method for the synergistic cultivation of surfactant-producing microbial communities and oil displacement agents in situ within oil reservoirs, including: Surfactant bacteria and nutrient solution are added to fermenter 1. The monitoring and display unit monitors and displays the pH value, dissolved oxygen value, biomass and liquid surfactant concentration in fermenter 1, and feeds back the pH value, dissolved oxygen value and biomass to PLC controller 2. The temperature control and heating component monitors the temperature in fermenter 1 and feeds back to PLC controller 2 in real time. PLC controller 2 compares temperature, dissolved oxygen value, and biomass with preset thresholds. When the dissolved oxygen value is lower than the preset threshold, the rotary agitator gas supply component is activated. When the biomass is not less than the preset threshold, the oil displacement agent dosage control component is activated. When the temperature is lower than the preset threshold, the temperature control heating component is activated for heating. When the dissolved oxygen value reaches the preset threshold, the rotary agitator gas supply component is turned off; when the biomass is less than the preset threshold, the oil displacement agent dosage control component is turned off; when the temperature reaches the preset threshold, the temperature control heating component is turned off.

[0043] In some embodiments, the rotary agitator gas supply assembly includes: a fixed box 6, a drive motor 601, a rotary pipe 602, an agitator pipe 603, and an air pump 606; the oil displacement agent dosage control assembly includes: an annular housing 5, a liquid flow sensor 501, a solenoid valve 502, and a liquid addition pipe; the monitoring and display unit includes a display 3, a surfactant concentration sensor 301, and a monitoring sensor 302; then it includes: Surfactant bacteria and nutrient solution are added to fermenter 1. Surfactant concentration sensor 301 and monitoring sensor 302 monitor the concentration of liquid surfactant, pH value, dissolved oxygen value and biomass in fermenter 1. Display 3 displays the concentration of liquid surfactant, pH value, dissolved oxygen value and biomass in fermenter 1 in real time. Temperature control heating component monitors the temperature in fermenter 1 and feeds it back to PLC controller 2 in real time. PLC controller 2 compares the temperature, dissolved oxygen value and biomass with preset thresholds. When the dissolved oxygen value is lower than the preset threshold, the drive motor 601 and air pump 606 are turned on. The drive motor 601 drives the rotating pipe 602 and stirring pipe 603. At the same time, the air pump 606 introduces outside air into the fermentation tank 1 through the rotating pipe 602 and stirring pipe 603 to increase oxygen. When the dissolved oxygen value reaches the preset threshold, the drive motor 601 and air pump 606 are turned off. When the biomass is not less than the preset threshold, the solenoid valve 502 is opened; the liquid flow sensor 501 monitors the oil displacement agent entering the fermenter 1 through the liquid addition pipe. When the flow rate reaches the preset flow rate value, the solenoid valve 502 is closed; after a 20-minute interval, when the real-time biomass is not less than the preset threshold, the solenoid valve 502 is opened again until the real-time biomass is less than the preset threshold. When the temperature is below the preset threshold, the temperature control heating component is turned on to heat the room; when the temperature reaches the preset threshold, the temperature control heating component is turned off.

[0044] In some embodiments: when the in-situ surfactant-producing bacteria and oil displacement agent co-culture device is used, surfactant bacteria and nutrient solution are added to the fermenter 1 through the feed pipe 101. The PLC controller 2 is preset to control the opening of the solenoid valve 502 when the biomass OD600 is greater than or equal to 5, and the flow rate value for controlling the closing of the solenoid valve 502 is preset. The PLC controller 2 is also preset to control the dissolved oxygen value for starting the air pump 606, and the opening and closing temperature value for controlling the electric heating jacket 4 is preset. The PLC controller 2 is then turned on, and the PLC controller 2 controls the drive motor 601 to start. The drive motor 601 drives the rotating tube 602 to rotate, and the rotating tube 602 drives multiple stirring tubes 603 to rotate, so as to stir and mix the inside of the fermenter 1 to make the liquid concentration consistent. Three sets of monitoring sensors 302 monitor the pH, dissolved oxygen, and biomass inside the fermenter 1, respectively, and transmit the corresponding values ​​to the PLC controller 2. When the dissolved oxygen is lower than the preset value, the PLC controller 2 controls the air pump 606 to start. The air pump 606 draws in external gas and supplies it into the fixed box 6. The gas enters the rotating pipe 602 through four air inlets 605, then flows into multiple stirring pipes 603, and then into the liquid through multiple jet nozzles 604. The oxygen-containing external gas is used to inject oxygen into the liquid. When the dissolved oxygen reaches the preset value, the PLC controller 2 controls the air pump 606 to automatically shut down. This achieves the effect of automatically injecting oxygen into the fermenter 1 when the dissolved oxygen is lower than the preset value, providing a favorable environment for the fermentation co-culture work, avoiding the reduction of culture effect due to low dissolved oxygen, and improving the co-culture effect. When the biomass OD600 is greater than or equal to 5, the PLC controller 2 controls the solenoid valve 502 to open. At this time, the oil displacement agent in the annular box 5 is added to the fermenter 1 through the liquid flow sensor 501 and the solenoid valve 502 for co-cultivation. The liquid flow sensor 501 detects the flow rate of the oil displacement agent and transmits the flow rate value to the PLC controller 2. When the preset flow rate value is reached, the PLC controller 2 controls the solenoid valve 502 to close, realizing the controlled addition of the oil displacement agent. The PLC controller 2 controls the amount of oil displacement agent added every 20 minutes according to the received biomass, until the biomass reaches the preset value range. If the received biomass OD600 does not reach the preset value range after 20 minutes, the controlled addition of the oil displacement agent is performed again until the set value range is finally reached and no more oil displacement agent is added. This achieves the effect of automatically controlling the amount of oil displacement agent added to the fermenter 1 according to the biomass for co-cultivation, without the need for manual addition, improving the degree of automation and convenience of use. Temperature sensor 401 monitors the temperature of the liquid inside fermenter 1 and transmits the temperature value to PLC controller 2. PLC controller 2 controls electric heating jacket 4 to automatically open and close according to the preset temperature value. Electric heating jacket 4 controls the temperature of the liquid inside fermenter 1 to ensure that it is in a good fermentation and cultivation temperature environment, thereby further improving the cultivation effect.

[0045] The PLC controller 2, liquid flow sensor 501, solenoid valve 502, pH sensor, DO sensor, biosensor, air pump 606, drive motor 601, electric heating mantle 4, and temperature sensor 401 mentioned in this application are well-known to those skilled in the art and belong to conventional methods or common knowledge. Those skilled in the art can arbitrarily select and match them according to their needs or convenience. In addition, the PLC controller 2 receives the biomass transmitted by the biosensor and controls the solenoid valve 502 to open according to the preset value, receives the flow value transmitted by the liquid flow sensor 501 and controls the solenoid valve 502 to close according to the preset value, receives the dissolved oxygen value transmitted by the DO sensor and controls the air pump 606 to start and stop according to the preset value, and receives the temperature transmitted by the temperature sensor 401 and controls the electric heating mantle 4 to start and stop according to the preset value. These are all basic programmable numerical control methods well-known to those skilled in the art and belong to conventional methods or common knowledge of programmers for programmable controllers. They will not be described in detail here.

[0046] This application discloses a device for the co-cultivation of surfactant-producing microbial communities and oil displacement agents in situ within an oil reservoir. The device includes a fermenter with four equally spaced, annular legs fixedly connected to its bottom, and a cover plate fixedly installed on its top. A feeding pipe, communicating with the interior of the fermenter, is embedded and fixed to the top left side of the cover plate. This application, through a series of structural features, enables uniform mixing within the fermenter, ensuring consistent liquid concentration. It facilitates real-time monitoring of dissolved oxygen levels and automatically injects oxygen into the fermenter when levels fall below a preset value, providing a favorable environment for co-cultivation and preventing reduced cultivation efficiency due to low dissolved oxygen levels. Furthermore, it allows for the automatic, controlled addition of oil displacement agents based on biomass, eliminating the need for manual addition and improving automation and convenience. Finally, it enables temperature control within the fermenter, ensuring a suitable fermentation environment.

[0047] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A device for the co-cultivation of surfactant-producing microbial communities and oil displacement agents in situ within an oil reservoir, characterized in that, The fermentation tank (1) is equipped with a PLC controller (2). The top of the fermentation tank (1) is equipped with a monitoring and display unit, an oil displacement agent control and addition component and a rotary stirring and gas supply component. The monitoring and display unit, the oil displacement agent control and addition component and the rotary stirring and gas supply component are all connected to the inside of the fermentation tank (1) and are all electrically connected to the PLC controller (2). The side wall of the fermentation tank (1) is surrounded by a temperature control and heating component, which is electrically connected to the PLC controller (2).

2. The in-situ reservoir surfactant-producing microbial community and oil displacement agent co-cultivation device according to claim 1, characterized in that, The fermentation tank (1) has four legs fixedly connected at equal intervals at the bottom and a cover plate fixedly installed on the top. The monitoring and display unit, the oil displacement agent control and addition component and the rotary stirring and gas supply component are all fixed on the cover plate. The cover plate is also equipped with a feeding pipe (101) and an exhaust pipe that are connected to the inside of the fermentation tank (1) and a discharge valve at the end of the fermentation tank (1).

3. The device for co-cultivating surfactant-producing bacteria and oil displacement agents in situ in oil reservoirs according to claim 1, characterized in that, The monitoring and display unit includes a display (3), a surfactant concentration sensor (301), and a monitoring sensor (302). The display (3), surfactant concentration sensor (301), and monitoring sensor (302) are all electrically connected to the PLC controller (2). The display (3) is located above the PLC controller (2). The monitoring sensor (302) is located at the top of the fermenter 1 and its detection end extends into the fermenter (1). The surfactant concentration sensor (301) is located on the side wall of the fermenter (1) and its detection end extends into the fermenter (1).

4. The in-situ surfactant-producing microbial community and oil displacement agent co-cultivation device according to claim 3, characterized in that, The monitoring sensors (302) include a pH sensor, a DO sensor, and a biosensor.

5. The device for co-cultivating surfactant-producing bacteria and oil displacement agents in situ in oil reservoirs according to claim 1, characterized in that, The oil displacement agent control addition component includes an annular box (5), which is set on the top of the fermentation tank (1) for storing the oil displacement agent. A liquid addition pipe is set at the bottom of the annular box (5), and a liquid flow sensor (501) is set on the liquid addition pipe. A solenoid valve (502) is fixedly connected to the bottom of the liquid flow sensor (501). The bottom of the solenoid valve (502) extends into the fermentation tank (1). The solenoid valve (502) and the liquid flow sensor (501) are both electrically connected to the PLC controller (2). The rotary stirring gas supply component is fixed on the top of the annular box (5) and extends into the fermentation tank (1).

6. The in-situ reservoir surfactant-producing microbial community and oil displacement agent co-cultivation device according to claim 1, characterized in that, The rotary agitator gas supply assembly includes a fixed box (6), which is fixed to the top of the oil displacement agent control addition assembly. A rotating tube (602) is rotatably fitted into the bottom of the fixed box (6). The rotating tube (602) extends through the oil displacement agent control addition assembly into the fermentation tank (1). Several agitator tubes (603), all located within the fermentation tank (1), are fixed to the outside of the rotating tube (602). Both the agitator tubes (603) and the rotating tubes (602) are hollow tubular structures, and their interiors are connected. The stirring tube (603) is provided with several jet holes (604), and the top of the four sides of the rotating tube (602) is provided with air inlets (605) located in the fixed box (6). The top of the fixed box (6) is fixedly installed with a drive motor (601) whose output shaft is fixedly connected to the top of the rotating tube (602). An air pump (606) is installed on one side of the fixed box (6), and the air outlet of the air pump (606) is connected to the fixed box (6). The drive motor (601) and the air pump (606) are both electrically connected to the PLC controller (2).

7. The device for co-cultivating surfactant-producing bacteria and oil displacement agents in situ in oil reservoirs according to claim 6, characterized in that, The bottom of the fixed box (6) has a circular through hole, and a sealed bearing is fixedly fitted inside the circular through hole. The inner ring of the sealed bearing is fixedly fitted to the outer side of the rotating tube (602).

8. The device for co-cultivating surfactant-producing bacteria and oil displacement agents in situ in oil reservoirs according to claim 1, characterized in that, The temperature control heating assembly includes an electric heating sleeve (4) and a temperature sensor (401). The electric heating sleeve (4) is fitted on the outside of the fermentation tank (1), and the temperature sensor (401) is set on the side wall of the fermentation tank (1). The detection end of the temperature sensor (401) extends into the inside of the fermentation tank (1). Both the temperature sensor (401) and the electric heating sleeve (4) are electrically connected to the PLC controller (2).

9. A method for synergistic cultivation of surfactant-producing microbial communities and oil displacement agents in situ of an oil reservoir, characterized in that, Based on the reservoir in-situ surfactant-producing microbial community and oil displacement agent co-cultivation device according to any one of claims 1 to 8, comprising: Surfactant bacteria and nutrient solution are added to the fermenter (1). The monitoring and display unit monitors and displays the pH value, dissolved oxygen value, biomass and liquid surfactant concentration in the fermenter (1), and feeds back the pH value, dissolved oxygen value and biomass to the PLC controller (2). The temperature control heating component monitors the temperature in the fermenter (1) and feeds back to the PLC controller (2) in real time. The PLC controller (2) compares the temperature, dissolved oxygen value and biomass with the preset threshold. When the dissolved oxygen value is lower than the preset threshold, the rotary drive stirring and gas supply component is turned on. When the biomass is not less than the preset threshold, the oil displacement agent control addition component is turned on. When the temperature is lower than the preset threshold, the temperature control heating component is turned on for heating. When the dissolved oxygen value reaches the preset threshold, the rotary agitator gas supply component is turned off; when the biomass is less than the preset threshold, the oil displacement agent dosage control component is turned off; when the temperature reaches the preset threshold, the temperature control heating component is turned off.

10. The method for synergistic cultivation of surfactant-producing microbial communities and oil displacement agents in situ of an oil reservoir according to claim 9, characterized in that, The rotary agitator gas supply assembly includes: a fixed box (6), a drive motor (601), a rotary tube (602), a stirring tube (603), and an air pump (606); the oil displacement agent dosage control assembly includes: an annular housing (5), a liquid flow sensor (501), a solenoid valve (502), and a liquid addition tube; the monitoring and display unit includes a display (3), a surfactant concentration sensor (301), and a monitoring sensor (302); and includes: Surfactant bacteria and nutrient solution are added to the fermenter (1). The surfactant concentration sensor (301) and monitoring sensor (302) monitor the concentration of liquid surfactant, pH value, dissolved oxygen value and biomass in the fermenter (1). The display (3) displays the concentration of liquid surfactant, pH value, dissolved oxygen value and biomass in the fermenter (1) in real time. The temperature control heating component monitors the temperature in the fermenter (1) and feeds it back to the PLC controller (2) in real time. The PLC controller (2) compares the temperature, dissolved oxygen value and biomass with the preset threshold. When the dissolved oxygen value is lower than the preset threshold, the drive motor (601) and air pump (606) are turned on. The drive motor (601) drives the rotating tube (602) and stirring tube (603). At the same time, the air pump (606) introduces outside air into the fermenter (1) through the rotating tube (602) and stirring tube (603) to increase oxygen. When the dissolved oxygen value reaches the preset threshold, the drive motor (601) and air pump (606) are turned off. When the biomass is not less than the preset threshold, the solenoid valve (502) is opened; the liquid flow sensor (501) monitors the oil displacement agent entering the fermenter (1) through the liquid addition pipe. When the flow reaches the preset flow value, the solenoid valve (502) is closed; after a 20-minute interval, when the real-time biomass is not less than the preset threshold, the solenoid valve (502) is opened again until the real-time biomass is less than the preset threshold. When the temperature is below the preset threshold, the temperature control heating component is turned on to heat the room; when the temperature reaches the preset threshold, the temperature control heating component is turned off.

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