Fokienia hodginsii heartwood essential oil as well as extraction process and application thereof in termite prevention and control

Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to fokienia hodginsii heartwood essential oil and an extraction process and application thereof in termite prevention and control. The extraction process of the Fujian cypress heartwood essential oil comprises the following steps: mixing Fujian cypress heartwood powder, NaCl and water, carrying out ultrasonic treatment, carrying out steam distillation extraction treatment on the obtained mixture, and collecting an upper oily substance in an extracting solution, so as to obtain the Fujian cypress heartwood essential oil, the content of NaCl in the mixture is 8 to 10 weight percent. The key point of the invention is that NaCl with a specific concentration is added when the ultrasonic-assisted steam distillation extraction process is adopted to extract the essential oil from the Fujian cypress heartwood, so that on one hand, the extraction rate of the Fujian cypress heartwood essential oil can be improved, and on the other hand, the extraction of some heavy oil components with good biological activity in the Fujian cypress heartwood is facilitated; therefore, the obtained centella asiatica heartwood essential oil shows excellent contact killing, repelling and fumigating effects on termites.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of plant extraction, and particularly relates to a Fokienia hodginsii heartwood essential oil, an extraction process thereof and application thereof in termite control. BACKGROUND

[0002] Fokienia hodginsii belongs to Fokienia of Cupressaceae and is a unique single-species plant in China with rich plant resources. At present, there are few reports on the extraction process of the essential oil of Fokienia hodginsii heartwood, and in the existing research, the essential oil of Fokienia hodginsii is usually extracted from Fokienia hodginsii leaves by using methods such as ultrasonic-assisted and steam distillation.

[0003] Coptotermes formosanus belongs to Blattaria, Zygoptera and Coptotermes, and is a kind of termite widely distributed in provinces south of the Yangtze River in China and is the most serious soil and wood ambulatory termite that damages house buildings, bridges and roadside trees. Pan J Q et al. have identified 33 compounds from the essential oil of Fokienia hodginsii (Research on the Components of Essential Oil from Fokienia hodginsii (Brief Report), Botany Bulletin, 1991, (04): 48-49). It has been found that α-pinene has strong contact killing, repellent and fumigation effects on Tribolium confusum adults. It has been found by Lu Y D et al. that the essential oil of Fokienia hodginsii has good biological activity on mosquitoes (Biological Activity of Essential Oil from Fokienia hodginsii on Mosquitoes, Journal of Pesticides Science, 2014, 16(03): 293-299). Although it has been found that the essential oil of Fokienia hodginsii has high insect resistance biological activity, there is no report on the anti-termite effect of the essential oil of Fokienia hodginsii. SUMMARY

[0004] The inventors of the present application have found through extensive and profound research that different Fokienia hodginsii raw materials (such as Fokienia hodginsii leaves and Fokienia hodginsii heartwood) may contain significantly different substances even if the same method is used, which leads to great differences in the functions of the essential oil of Fokienia hodginsii. The essential oil of Fokienia hodginsii is usually extracted from Fokienia hodginsii leaves, and the anti-termite effect of the essential oil is poor.

[0005] One of the purposes of the present application is to provide an extraction process of Fokienia hodginsii heartwood essential oil in view of the poor anti-termite effect of the essential oil of Fokienia hodginsii in the prior art, and the Fokienia hodginsii heartwood essential oil obtained by the extraction process has significant contact killing, repellent and fumigation effects on termites.

[0006] Specifically, the extraction process of the Fokienia Hodginsii heartwood essential oil comprises the following steps: mixing Fokienia Hodginsii heartwood powder, NaCl and water, and then performing ultrasonic treatment, performing water vapor distillation extraction treatment on the obtained mixture, and collecting upper oil-like substances in the extraction liquid, so as to obtain the Fokienia Hodginsii heartwood essential oil; the concentration of the NaCl solution prepared by the NaCl and water is 8-12 wt%.

[0007] In a preferred embodiment, the use amount ratio of the Fokienia Hodginsii heartwood powder to water is 1g:(2-6)mL.

[0008] In a preferred embodiment, the ultrasonic treatment is performed under the following conditions: the ultrasonic power is 300-500W, and the ultrasonic time is 20-60min.

[0009] In a preferred embodiment, the ultrasonic power is 300-400W, and the ultrasonic time is 20-40min.

[0010] In a preferred embodiment, the water vapor distillation extraction treatment is performed at a temperature of 80-100℃ for 6-10h.

[0011] In a preferred embodiment, the particle size of the Fokienia Hodginsii heartwood is 0.1-0.4mm.

[0012] In a preferred embodiment, the upper oil-like substances are further subjected to drying treatment by using anhydrous sodium sulfate and / or anhydrous magnesium sulfate.

[0013] The second object of the present application is to provide the Fokienia Hodginsii heartwood essential oil prepared by the above-mentioned extraction process.

[0014] The third object of the present application is to provide the application of the above-mentioned Fokienia Hodginsii heartwood essential oil in termite control.

[0015] In a preferred embodiment, the application comprises providing a termite control agent containing the Fokienia Hodginsii heartwood essential oil of claim 8.

[0016] Beneficial effects: The key of the present application is that the ultrasonic-assisted water vapor distillation extraction process is used to extract the essential oil from the Fokienia Hodginsii heartwood, and a specific concentration of NaCl is added, which can not only improve the extraction rate of the Fokienia Hodginsii heartwood essential oil, but also is beneficial to the extraction of some heavy oil components with good biological activity in the Fokienia Hodginsii heartwood, so that the obtained Fokienia Hodginsii heartwood essential oil shows excellent repellent, repellent and fumigation effects on termites, and provides a basis for the development of plant source termite control agents. DETAILED DESCRIPTION

[0017] The embodiments of the present invention are described in detail below. The embodiments described below are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention. Furthermore, unless otherwise specified, all embodiments and optional embodiments of the present invention can be combined with each other to form new technical solutions, and such technical solutions should be considered to be included in the disclosure of the present invention.

[0018] The extraction process for Fujian cypress heartwood essential oil provided by this invention includes the following steps: mixing Fujian cypress heartwood powder, NaCl, and water, followed by ultrasonic treatment; then subjecting the resulting mixture to steam distillation extraction; and collecting the upper oily substance in the extract to obtain Fujian cypress heartwood essential oil. The concentration of the NaCl solution prepared by mixing NaCl and water is 8-12 wt%, such as 8 wt%, 9 wt%, 10 wt%, 11 wt%, 12 wt%, or any value between these values. Controlling the NaCl concentration within the above-mentioned preferred range offers advantages such as high essential oil yield, good stability, and excellent synergy with other extraction conditions. This is because 8-12 wt% NaCl provides suitable ionic strength to optimize the salting-out effect, reduce essential oil dissolution loss, promote separation, and also synergizes with ultrasound to improve the extraction effect.

[0019] In this invention, the preferred ratio of the Fujian cypress heartwood powder to water is 1g:(2~6)mL, such as 1g:2mL, 1g:3mL, 1g:4mL, 1g:5mL, 1g:6mL or any ratio between them.

[0020] In this invention, the preferred conditions for ultrasonic treatment include: ultrasonic power of 300-500W, such as 300W, 350W, 400W, 450W, 500W, or any value between them; and ultrasonic time of 20-60min, such as 20min, 30min, 40min, 50min, 60min, or any value between them. Controlling the ultrasonic treatment conditions within the above-preferred range enables the extraction process to achieve a high essential oil extraction rate.

[0021] In this invention, the ultrasonic power is further preferably 300~400W, and the ultrasonic time is further preferably 20~40min, which is more conducive to further improving the extraction rate of Fujian cypress essential oil.

[0022] In this invention, the temperature of the steam distillation extraction process is preferably 80~100℃, such as 80℃, 85℃, 90℃, 95℃, 100℃ or any value between them; the time is preferably 6~10h, such as 6h, 7h, 7.5h, 8h, 8.5h, 9h, 10h or any value between them.

[0023] In this invention, the particle size of the Fujian cypress heartwood is preferably 0.1~0.4mm, such as 0.1mm, 0.2mm, 0.3mm, 0.4mm or any value between them.

[0024] In this invention, the upper oily substance preferably includes a drying treatment using anhydrous sodium sulfate and / or anhydrous magnesium sulfate.

[0025] The present invention will be described in detail below through specific embodiments. These embodiments are intended to explain the invention and should not be construed as limiting it. Where specific techniques or conditions are not specified in the embodiments, they are performed according to the techniques or conditions described in the literature in the art or according to the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be obtained commercially.

[0026] The heartwood and leaves of *Platycladus orientalis* used in the following examples and comparative examples were collected from the Fengtian State-owned Forest Farm in Anxi County, Quanzhou City, Fujian Province. The termite species tested in the following test examples was *Termitomyces subulata*, provided by Guangdong Maoming Jingfeng Pest Control Service Co., Ltd.

[0027] Example 1 This embodiment illustrates an extraction process for essential oil from the heartwood of *Cypress fusiforme*, the specific process of which is as follows: After being air-dried, the heartwood of *Platycladus orientalis* was pulverized and sieved to obtain powder with a particle size of 0.1–0.4 mm. A 10 wt% NaCl solution was prepared by mixing 2000 mL of ultrapure water with NaCl. 500 g of *Platycladus orientalis* heartwood powder was weighed and mixed with the NaCl solution (2000 mL) in a 5000 mL round-bottom flask. The mixture was then ultrasonically treated for 30 min in an ultrasonic extractor with an ultrasonic power of 350 W. The round-bottom flask was then transferred to a heating mantle and heated to 90 °C for steam distillation extraction for 8 h. After cooling to room temperature, the upper oily substance in the extract was collected by separation and dried with anhydrous sodium sulfate to obtain *Platycladus orientalis* heartwood essential oil, denoted as F-1.

[0028] Example 2 This embodiment illustrates an extraction process for essential oil from the heartwood of *Cypress fusiforme*, the specific process of which is as follows: After being air-dried, the heartwood of *Platycladus orientalis* was pulverized and sieved to obtain powder with a particle size of 0.1–0.4 mm. A 10 wt% NaCl solution was prepared by mixing 2000 mL of ultrapure water with NaCl. 500 g of *Platycladus orientalis* heartwood powder was weighed and mixed with the NaCl solution (2000 mL) in a 5000 mL round-bottom flask. The mixture was then ultrasonically treated in an ultrasonic extractor with an ultrasonic power of 300 W for 40 min. The round-bottom flask was then transferred to a heating mantle and heated to 90 °C for steam distillation extraction for 8 h. After cooling to room temperature, the upper oily substance in the extract was collected by separation and dried with anhydrous sodium sulfate to obtain *Platycladus orientalis* heartwood essential oil, denoted as F-2.

[0029] Example 3 This embodiment illustrates an extraction process for essential oil from the heartwood of *Cypress fusiforme*, the specific process of which is as follows: After being air-dried, the heartwood of *Platycladus orientalis* was pulverized and sieved to obtain powder with a particle size of 0.1–0.4 mm. A 10 wt% NaCl solution was prepared by mixing 2000 mL of ultrapure water with NaCl. 500 g of *Platycladus orientalis* heartwood powder was weighed and mixed with the NaCl solution (2000 mL) in a 5000 mL round-bottom flask. The mixture was then ultrasonically treated for 20 min in an ultrasonic extractor with an ultrasonic power of 400 W. The round-bottom flask was then transferred to a heating mantle and heated to 90 °C for steam distillation extraction for 8 h. After cooling to room temperature, the upper oily substance in the extract was collected by separation and dried with anhydrous sodium sulfate to obtain *Platycladus orientalis* heartwood essential oil, denoted as F-3.

[0030] Example 4 This embodiment illustrates an extraction process for essential oil from the heartwood of *Cypress fusiforme*, the specific process of which is as follows: After being air-dried, the heartwood of *Platycladus orientalis* was pulverized and sieved to obtain powder with a particle size of 0.1–0.4 mm. An 8 wt% NaCl solution was prepared by mixing 2000 mL of ultrapure water with NaCl. 500 g of *Platycladus orientalis* heartwood powder was weighed and mixed with the NaCl solution (2000 mL) in a 5000 mL round-bottom flask. The mixture was then ultrasonically treated for 30 min in an ultrasonic extractor with an ultrasonic power of 400 W. The flask was then transferred to a heating mantle and heated to 90 °C for steam distillation extraction for 8 h. After cooling to room temperature, the upper oily layer of the extract was collected by separation and dried with anhydrous sodium sulfate to obtain *Platycladus orientalis* heartwood essential oil, denoted as F-4.

[0031] Example 5 This embodiment illustrates an extraction process for a reference Fujian cypress heartwood essential oil, the specific process of which is as follows: After being air-dried, the heartwood of *Platycladus orientalis* was pulverized and sieved to obtain powder with a particle size of 0.1–0.4 mm. A 12 wt% NaCl solution was prepared by mixing 2000 mL of ultrapure water with NaCl. 500 g of *Platycladus orientalis* heartwood powder was weighed and mixed with the NaCl solution (2000 mL) in a 5000 mL round-bottom flask. The mixture was then ultrasonically treated for 30 min in an ultrasonic extractor with an ultrasonic power of 400 W. The round-bottom flask was then transferred to a heating mantle and heated to 90 °C for steam distillation extraction for 8 h. After cooling to room temperature, the upper oily substance in the extract was collected by separation and dried with anhydrous sodium sulfate to obtain *Platycladus orientalis* heartwood essential oil, denoted as F-5.

[0032] Comparative Example 1 This comparative example illustrates an extraction process for a reference Fujian cypress heartwood essential oil, the specific process of which is as follows: After being air-dried, the heartwood of *Platycladus orientalis* was pulverized and sieved to obtain powder with a particle size of 0.1–0.4 mm. 500 g of the powder was weighed and mixed with 2000 mL of ultrapure water in a 5000 mL round-bottom flask. The mixture was then ultrasonically treated for 30 min in an ultrasonic extractor with an ultrasonic power of 350 W. The flask was then transferred to a heating mantle and heated to 90 °C for steam distillation extraction for 8 h. After cooling to room temperature, the upper oily layer of the extract was collected by separation and dried with anhydrous sodium sulfate to obtain the reference *Platycladus orientalis* heartwood essential oil, denoted as DF-1.

[0033] Comparative Example 2 This comparative example illustrates an extraction process for a reference Fujian cypress heartwood essential oil, the specific process of which is as follows: After being air-dried, the leaves of *Platycladus orientalis* were pulverized and sieved to obtain powder with a particle size of 0.1–0.4 mm. 500 g of the powder was weighed and mixed with 2000 mL of ultrapure water in a 5000 mL round-bottom flask. The mixture was then ultrasonically treated for 30 min in an ultrasonic extractor with an ultrasonic power of 350 W. The flask was then transferred to a heating mantle and heated to 90 °C for steam distillation extraction for 8 h. After cooling to room temperature, the upper oily layer of the extract was collected by separation and dried with anhydrous sodium sulfate to obtain the reference *Platycladus orientalis* heartwood essential oil, denoted as DF-2.

[0034] Comparative Example 3 This comparative example illustrates an extraction process for a reference Fujian cypress heartwood essential oil, the specific process of which is as follows: After being air-dried, the heartwood of *Platycladus orientalis* was pulverized and sieved to obtain powder with a particle size of 0.1–0.4 mm. A 3 wt% NaCl solution was prepared by mixing 2000 mL of ultrapure water with NaCl. 500 g of *Platycladus orientalis* heartwood powder was weighed and mixed with the NaCl solution (2000 mL) in a 5000 mL round-bottom flask. The mixture was then ultrasonically treated for 30 min in an ultrasonic extractor with an ultrasonic power of 350 W. The round-bottom flask was then transferred to a heating mantle and heated to 90 °C for steam distillation extraction for 8 h. After cooling to room temperature, the upper oily substance in the extract was collected by separation and dried with anhydrous sodium sulfate to obtain *Platycladus orientalis* heartwood essential oil, denoted as DF-3.

[0035] Test Example 1: Essential Oil Yield According to formula (1): essential oil yield (%) = essential oil mass (g) ÷ Fujian cypress raw material mass (g) × 100, the yield of Fujian cypress essential oil prepared in the above examples and comparative examples was calculated. The results are shown in Table 1. Compared with the comparative example, the essential oil yield obtained by the method of the present invention is higher.

[0036] Table 1

[0037] Test Example 2: Termite Resistance Test 1. Test on the contact toxicity of Fujian cypress essential oil against subterranean termites. (1-1) Test Method: In the experimental preparation stage, a 3.5cm diameter qualitative filter paper was laid flat on the bottom of a 3.8cm diameter petri dish. Using acetone as a solvent, Fujian cypress essential oil was diluted to five different concentration gradients: 0.0mg / g, 1.0mg / g, 2.5mg / g, 5.0mg / g, and 10.0mg / g. 200μL of each concentration of essential oil dilution was accurately measured using a pipette and evenly added to the filter paper. After the solvent evaporated and the paper was dried, the petri dish was covered. Thirty healthy and vigorous termite worker ants were introduced into each petri dish. The experimental environment was set at a temperature of (27±2)℃, a relative humidity of 75±5%, and kept in darkness. Subsequently, observations were conducted daily, and any dead worker ants were promptly removed from the petri dishes. The number of deaths was recorded in detail, and observation continued until all termites had died. Each concentration of essential oil dilution was tested in triplicate, with a group using acetone-treated filter paper as a blank control.

[0038] (1-2) Test Results: The corrected mortality rate was calculated according to formulas (2), (3), and (4). The results are shown in Table 2. Table 2 shows that the contact toxicity of Fujian cypress essential oil against subterranean termites significantly increased with prolonged treatment time and concentration. After 24 hours of treatment at a concentration of 10.0 mg / g, the corrected mortality rate reached 95.56%. When the treatment time was 24 hours, the regression equation between the concentration (x) of Fujian cypress essential oil F-1 and the contact toxicity rate (y) of subterranean termites was: y = 2.0664x + 10.021, LC 50 =1.87mg / g (LC) 50 (This represents the concentration at which 50% of termite individuals die). The corrected mortality rate of the Fujian cypress essential oil obtained in the examples after treatment at a concentration of 10.0 mg / g for 24 hours was higher than that of the comparative example.

[0039] Formula (2): Mortality rate of the treatment group = (Number of dead insects in the treatment group / Number of insects in the treatment group) × 100% Formula (3): Mortality rate of blank control = Number of dead insects in blank control / Number of dead insects in treatment × 100% Formula (4): Corrected mortality rate = (treatment group mortality rate - blank control mortality rate) / (1 - blank control mortality rate) × 100% Table 2

[0040] Note: Values ​​in the table are mean ± standard error. If two means in the same column have the same letter, it indicates no significant difference; if the letters are different, it indicates a difference at the P < 0.05 level.

[0041] 2. Fumigation effect test of Fujian cypress essential oil on subterranean termites. (2-1) Test Method: During the fumigation experiment, filter paper with a diameter of 3.5 cm was attached to the cap of a 1L glass bottle. The dosage gradient of Fujian cypress essential oil for fumigating subterranean termites was 0.16 μL, 0.41 μL, 0.81 μL, and 1.63 μL. The essential oil was evenly dripped onto the filter paper, and after the solvent evaporated and dried, the glass bottle cap was closed for later use. Thirty healthy and vigorous subterranean termite worker ants were introduced into each petri dish. The bottle cap was quickly closed, and the sides of the glass bottle cap were wrapped with plastic wrap to isolate it from the outside air. The experimental environment was set at a temperature of (27±2)℃, a relative humidity of 75±5%, and kept in darkness. Subsequently, observations were made once a day at regular intervals, and the number of deaths was recorded in detail until all termites died. Each concentration treatment was repeated three times, and a group with filter paper treated with acetone was set up as a blank control.

[0042] (2-2) Test Results: The corrected mortality rate was calculated according to formulas (2), (3), and (4). The results are shown in Table 3. Table 3 shows that the fumigation effect of Fujian cypress essential oil on termites significantly increased with prolonged treatment time and concentration. After 24 hours of treatment at a dosage of 1.63 μL, the corrected mortality rate reached 98.89%. When the treatment lasted 24 hours, the fumigation regression equation between the concentration (x) of Fujian cypress essential oil F-1 and the fumigation mortality rate (y) of termites was: y = 17.209x + 5.8404, LC 50 =0.334μL (LC) 50 (This represents the concentration at which 50% of termite individuals die). The corrected mortality rate of the Fujian cypress oil obtained in the examples, after treatment at a dosage of 1.63 μL for 24 hours, was higher than that of the comparative example. Table 3

[0043] Note: Values ​​in the table are mean ± standard error. If two means in the same column have the same letter, it indicates no significant difference; if the letters are different, it indicates a difference at the P < 0.05 level.

[0044] 3. Test on the repellent effect of Fujian cypress essential oil on subterranean termites. (3-1) Test Method: Prepare a circular filter paper with a diameter of 9cm and cut it in half along the diameter. One half of the filter paper was immersed in a diluted solution of Hovenia dulcis essential oil (Hovenia dulcis essential oil concentrations were 0.0mg / g, 1.0mg / g, 2.5mg / g, 5.0mg / g, and 10.0mg / g), while the other half was immersed in acetone solution as a control sample. After immersion, the two halves of the filter paper were removed and air-dried in a natural environment. Once completely dry, the two halves were glued together with transparent tape and placed tightly at the bottom of a 9cm diameter petri dish. Thirty worker termites were placed in the center of the petri dish, and then the petri dish was placed in a constant temperature incubator. The incubator environment was set at a temperature of 27±2℃ and a relative humidity of 75±5%. The distribution and number of termites in the two different treatment areas of the filter paper were observed and recorded at 2h, 4h, 6h, 8h, 10h, 12h, and 24h after the start of the experiment.

[0045] (3-2) Test Results: The repellency rate was calculated according to formula (5) based on the observation data, and the results are shown in Table 4. Table 4 shows that Fujian cypress essential oil has a strong repellent effect on termites. Its repellency effect increases with the extension of treatment time and the increase of treatment dosage. Especially at a treatment dosage of 10 mg / g, the repellency activity is above level IV, and after 24 hours of treatment, the repellency rate reaches 98.9%, with repellency activity reaching level V. The Fujian cypress essential oil obtained in the example achieved a repellency rate of level V after 24 hours of treatment at a concentration of 10.0 mg / g, while the comparative example only achieved level IV.

[0046] Formula (5): Repellency rate = (Number of control crickets - Number of treated crickets) / Number of control crickets × 100%. The grading standards for repellency activity are as follows: Grade 0: No repellency activity; Grade I: Repellency rate 0~20%; Grade II: Repellency rate 20.1~40.0%; Grade III: Repellency rate 40.1~60.0%; Grade IV: Repellency rate 60.1~80.0%; Grade V: Repellency rate 80.1~100%.

[0047] Table 4

[0048]

[0049] In summary, the test results show that the Fujian cypress heartwood essential oil prepared by the extraction process of this invention not only has a higher essential oil yield, but also has better contact killing, fumigation and repellency effects on termites than the comparative example, and has a significant killing effect.

[0050] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention without departing from the principles and spirit of the present invention.

Claims

1. A process for extracting essential oil from the heartwood of *Cypress fusiforme*, characterized in that, The extraction process includes the following steps: mixing Fujian cypress heartwood powder, NaCl, and water, and then subjecting the mixture to ultrasonic treatment. The resulting mixture is then subjected to steam distillation extraction, and the upper oily substance in the extract is collected to obtain Fujian cypress heartwood essential oil. The concentration of the NaCl solution prepared by mixing NaCl and water is 8-12 wt%.

2. The extraction process for Fujian cypress heartwood essential oil according to claim 1, characterized in that, The ratio of the Fujian cypress heartwood powder to water is 1g:(2~6)mL.

3. The extraction process for Fujian cypress heartwood essential oil according to claim 1, characterized in that, The conditions for ultrasonic treatment include: ultrasonic power of 300~500W and ultrasonic time of 20~60min.

4. The extraction process for Fujian cypress heartwood essential oil according to claim 3, characterized in that, The ultrasonic power is 300~400W, and the ultrasonic time is 20~40min.

5. The extraction process for Fujian cypress heartwood essential oil according to claim 1, characterized in that, The steam distillation extraction process is carried out at a temperature of 80-100℃ for 6-10 hours.

6. The extraction process for essential oil from the heartwood of *Platycladus orientalis* according to claim 1, characterized in that, The particle size of the heartwood of the Fujian cypress is 0.1~0.4mm.

7. The extraction process for Fujian cypress heartwood essential oil according to claim 1, characterized in that, The upper oily substance also includes drying treatment using anhydrous sodium sulfate and / or anhydrous magnesium sulfate.

8. Fujian cypress heartwood essential oil prepared by the extraction process of Fujian cypress heartwood essential oil according to any one of claims 1 to 7.

9. The application of the Fujian cypress heartwood essential oil according to claim 8 in termite control.

10. The application of Fujian cypress heartwood essential oil according to claim 9 in termite control, characterized in that, The application includes providing a termite control agent containing the Fujian cypress heartwood essential oil as described in claim 8.

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